SitesBLAST
Comparing WP_043745229.1 NCBI__GCF_000009985.1:WP_043745229.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3pm9A Crystal structure of a putative dehydrogenase (rpa1076) from rhodopseudomonas palustris cga009 at 2.57 a resolution
53% identity, 98% coverage: 7:466/469 of query aligns to 10:465/465 of 3pm9A
- active site: A149 (= A148), L159 (≠ N158)
- binding flavin-adenine dinucleotide: P69 (= P66), Q70 (= Q67), G71 (= G68), G72 (= G69), N73 (= N70), T74 (= T71), G75 (= G72), L76 (= L73), G79 (= G76), Q80 (≠ G77), L91 (≠ T90), L133 (= L132), G134 (= G133), A135 (= A134), C139 (= C138), T140 (≠ R139), G142 (= G141), G143 (= G142), S146 (= S145), T147 (= T146), A149 (= A148), G150 (= G149), E200 (= E199), G201 (= G200), I205 (= I204), I206 (≠ V205), E423 (= E424)
6lpxA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with 2-oxoglutarate (2-og) (see paper)
38% identity, 99% coverage: 4:466/469 of query aligns to 14:464/466 of 6lpxA
- binding 2-oxoglutaric acid: R333 (= R328), T337 (≠ P332), K348 (= K343), Y379 (≠ F376), H381 (= H378), H388 (= H385), H423 (= H425)
- binding flavin-adenine dinucleotide: W39 (≠ E30), P75 (= P66), Q76 (= Q67), G77 (= G68), G78 (= G69), N79 (= N70), T80 (= T71), G81 (= G72), M82 (≠ L73), G85 (= G76), S86 (≠ G77), L139 (= L132), G140 (= G133), A141 (= A134), C145 (= C138), G149 (= G142), N150 (= N143), A152 (≠ S145), T153 (= T146), G157 (= G150), G207 (= G200), I212 (≠ V205), E422 (= E424), N459 (= N461)
- binding zinc ion: H381 (= H378), H388 (= H385), E422 (= E424)
6lpuA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with l-2-hydroxyglutarate (l-2-hg) (see paper)
38% identity, 99% coverage: 4:466/469 of query aligns to 14:464/466 of 6lpuA
- binding flavin-adenine dinucleotide: W39 (≠ E30), P75 (= P66), G77 (= G68), G78 (= G69), N79 (= N70), T80 (= T71), G81 (= G72), G85 (= G76), S86 (≠ G77), L139 (= L132), G140 (= G133), A141 (= A134), C145 (= C138), H146 (≠ R139), G148 (= G141), G149 (= G142), N150 (= N143), A152 (≠ S145), T153 (= T146), A155 (= A148), E206 (= E199), G207 (= G200), I211 (= I204), I212 (≠ V205), E422 (= E424), N459 (= N461)
- binding (2s)-2-hydroxypentanedioic acid: R333 (= R328), T337 (≠ P332), K348 (= K343), Y379 (≠ F376), H381 (= H378), H388 (= H385), H423 (= H425)
- binding zinc ion: H381 (= H378), H388 (= H385), E422 (= E424)
6lpqA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-malate (d-mal) (see paper)
38% identity, 99% coverage: 4:466/469 of query aligns to 14:464/466 of 6lpqA
- binding flavin-adenine dinucleotide: W39 (≠ E30), P75 (= P66), G77 (= G68), G78 (= G69), N79 (= N70), T80 (= T71), G81 (= G72), G85 (= G76), S86 (≠ G77), L139 (= L132), G140 (= G133), A141 (= A134), C145 (= C138), H146 (≠ R139), G149 (= G142), N150 (= N143), A152 (≠ S145), T153 (= T146), A155 (= A148), G157 (= G150), E206 (= E199), G207 (= G200), I211 (= I204), I212 (≠ V205), E422 (= E424), N459 (= N461)
- binding d-malate: M82 (≠ L73), R333 (= R328), T337 (≠ P332), K348 (= K343), Y379 (≠ F376), H381 (= H378), H388 (= H385), E422 (= E424), H423 (= H425)
- binding zinc ion: H381 (= H378), H388 (= H385), E422 (= E424)
6lppA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-2-hydroxyglutarate (d-2-hg) (see paper)
38% identity, 99% coverage: 4:466/469 of query aligns to 14:464/466 of 6lppA
- binding (2R)-2-hydroxypentanedioic acid: R333 (= R328), T337 (≠ P332), K348 (= K343), Y379 (≠ F376), H381 (= H378), H388 (= H385), N390 (= N387), H423 (= H425)
- binding flavin-adenine dinucleotide: W39 (≠ E30), P75 (= P66), G77 (= G68), G78 (= G69), N79 (= N70), T80 (= T71), G81 (= G72), M82 (≠ L73), G85 (= G76), S86 (≠ G77), L139 (= L132), G140 (= G133), A141 (= A134), C145 (= C138), G149 (= G142), N150 (= N143), A152 (≠ S145), T153 (= T146), A155 (= A148), G157 (= G150), G207 (= G200), I212 (≠ V205), E422 (= E424), H423 (= H425)
- binding zinc ion: H381 (= H378), H388 (= H385), E422 (= E424)
6lpnB Crystal structure of human d-2-hydroxyglutarate dehydrogenase in apo form (see paper)
38% identity, 99% coverage: 4:466/469 of query aligns to 15:465/467 of 6lpnB
- binding flavin-adenine dinucleotide: W40 (≠ E30), P76 (= P66), G78 (= G68), G79 (= G69), N80 (= N70), T81 (= T71), G82 (= G72), M83 (≠ L73), G86 (= G76), S87 (≠ G77), L140 (= L132), A142 (= A134), C146 (= C138), H147 (≠ R139), G150 (= G142), N151 (= N143), A153 (≠ S145), T154 (= T146), G208 (= G200), I212 (= I204), I213 (≠ V205), E423 (= E424), N460 (= N461)
Q8N465 D-2-hydroxyglutarate dehydrogenase, mitochondrial; EC 1.1.99.39 from Homo sapiens (Human) (see 7 papers)
38% identity, 99% coverage: 4:466/469 of query aligns to 67:517/521 of Q8N465
- S109 (≠ T47) to W: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs142050154
- N127 (≠ V65) to K: in D2HGA1; uncertain significance; complete loss of catalytic activity; dbSNP:rs762857195
- G131 (= G69) to V: in D2HGA1; uncertain significance; complete loss of catalytic activity
- I147 (≠ V87) to S: in D2HGA1; uncertain significance; severe phenotype; almost complete loss of catalytic activity; dbSNP:rs121434361
- M153 (≠ L93) to T: in D2HGA1; uncertain significance; significant loss of catalytic activity; to V: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs1432270139
- C172 (= C112) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs773735172
- P189 (= P129) to L: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs587783517
- A205 (≠ S145) to V: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs750889931
- A231 (≠ P171) to V: in D2HGA1; uncertain significance; significant loss of catalytic activity
- G233 (= G173) to S: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs374535734
- D375 (≠ S317) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs267606759
- R386 (= R328) binding ; binding ; binding ; mutation to A: Loss of catalytic activity.
- T390 (≠ P332) binding ; binding ; mutation to A: Significantly reduced catalytic activity.
- V399 (≠ S341) to M: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746519212
- K401 (= K343) binding ; binding ; mutation to A: Loss of catalytic activity.
- R419 (= R359) to H: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs199908032
- A426 (= A366) to T: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs146578303
- H434 (= H378) binding ; mutation to A: Loss of catalytic activity.
- G436 (= G380) to V: slight reduction in catalytic activity
- N439 (= N383) to D: in D2HGA1; uncertain significance; mild phenotype; moderate reduction in catalytic activity; dbSNP:rs121434362
- H441 (= H385) binding ; mutation to A: Loss of catalytic activity.
- N443 (= N387) binding ; mutation to A: Significantly reduced catalytic activity.
- V444 (≠ L388) to A: in D2HGA1; uncertain significance; severe phenotype; significant reduction in catalytic activity; dbSNP:rs121434360
- A446 (≠ Q390) to V: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746956176
- E475 (= E424) binding ; mutation to A: Loss of catalytic activity.
- H476 (= H425) binding ; binding ; binding ; mutation to A: Loss of catalytic activity.
- G477 (= G426) to R: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs1453924640
Sites not aligning to the query:
- 15 R → G: in dbSNP:rs4675887
- 169:521 natural variant: Missing (in D2HGA1; uncertain significance)
- 400:521 natural variant: Missing (in D2HGA1; uncertain significance; complete loss of catalytic activity)
P39976 D-2-hydroxyglutarate--pyruvate transhydrogenase DLD3; D-2HG--pyruvate transhydrogenase DLD3; (R)-2-hydroxyglutarate--pyruvate transhydrogenase; D-lactate dehydrogenase [cytochrome] 3; D-lactate ferricytochrome C oxidoreductase; D-LCR; EC 1.1.99.40; EC 1.1.2.4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
32% identity, 95% coverage: 22:466/469 of query aligns to 52:496/496 of P39976
Sites not aligning to the query:
- 17 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
7qh2C Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
32% identity, 91% coverage: 41:467/469 of query aligns to 48:465/467 of 7qh2C
- binding flavin-adenine dinucleotide: V73 (≠ P66), G75 (= G68), S76 (≠ G69), G77 (≠ N70), T78 (= T71), G79 (= G72), L80 (= L73), A83 (≠ G76), C84 (≠ G77), P137 (≠ L132), G138 (= G133), E139 (= E135), A142 (≠ C138), T143 (≠ R139), G146 (= G142), N147 (= N143), S149 (= S145), T150 (= T146), A152 (= A148), G153 (= G149), E203 (= E199), G204 (= G200), I209 (≠ V205), E422 (= E424), H423 (= H425)
- binding fe (iii) ion: H377 (= H378), H384 (= H385), E422 (= E424)
P9WIT1 Uncharacterized FAD-linked oxidoreductase Rv2280; EC 1.-.-.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
31% identity, 98% coverage: 7:466/469 of query aligns to 8:456/459 of P9WIT1
- K354 (≠ E355) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
8jdzA Crystal structure of mldhd in complex with 2-keto-3-methylvaleric acid (see paper)
32% identity, 99% coverage: 3:466/469 of query aligns to 5:454/454 of 8jdzA
- binding (3S)-3-methyl-2-oxopentanoic acid: R318 (= R328), W322 (≠ I331), H369 (= H378), H376 (= H385), H413 (= H425)
- binding flavin-adenine dinucleotide: E32 (= E30), P68 (= P66), G70 (= G68), T71 (≠ G69), G72 (≠ N70), T73 (= T71), G74 (= G72), G78 (= G76), V79 (≠ G77), L90 (≠ T90), P132 (≠ L132), G133 (= G133), A134 (= A134), G140 (= G142), M141 (≠ N143), A143 (≠ S145), T144 (= T146), A146 (= A148), S147 (≠ G149), E200 (= E199), G201 (= G200), I206 (≠ V205), W322 (≠ I331), E412 (= E424), H413 (= H425), N449 (= N461)
- binding manganese (ii) ion: H369 (= H378), H376 (= H385), E412 (= E424)
8jdyA Crystal structure of mldhd in complex with 2-ketoisocaproic acid (see paper)
32% identity, 99% coverage: 3:466/469 of query aligns to 5:454/454 of 8jdyA
- binding 2-oxo-4-methylpentanoic acid: R318 (= R328), W322 (≠ I331), S336 (vs. gap), H369 (= H378), H376 (= H385), H413 (= H425)
- binding flavin-adenine dinucleotide: P68 (= P66), G70 (= G68), T71 (≠ G69), G72 (≠ N70), T73 (= T71), G74 (= G72), G78 (= G76), V79 (≠ G77), L90 (≠ T90), P132 (≠ L132), G133 (= G133), A134 (= A134), G140 (= G142), M141 (≠ N143), A143 (≠ S145), T144 (= T146), A146 (= A148), S147 (≠ G149), E200 (= E199), G201 (= G200), I206 (≠ V205), E412 (= E424), N449 (= N461)
- binding manganese (ii) ion: H369 (= H378), H376 (= H385), E412 (= E424)
8jdeA Crystal structure of mldhd in complex with d-lactate (see paper)
31% identity, 99% coverage: 3:466/469 of query aligns to 5:455/455 of 8jdeA
- binding flavin-adenine dinucleotide: P68 (= P66), G70 (= G68), T71 (≠ G69), G72 (≠ N70), T73 (= T71), G74 (= G72), G78 (= G76), V79 (≠ G77), L90 (≠ T90), P132 (≠ L132), G133 (= G133), A134 (= A134), G140 (= G142), M141 (≠ N143), A143 (≠ S145), T144 (= T146), A146 (= A148), S147 (≠ G149), E200 (= E199), G201 (= G200), I206 (≠ V205), W322 (= W325), E413 (= E424), H414 (= H425), N450 (= N461)
- binding lactic acid: R318 (= R321), H369 (= H378), H376 (= H385), H414 (= H425)
- binding manganese (ii) ion: H369 (= H378), H376 (= H385), E413 (= E424)
8jdxA Crystal structure of mldhd in complex with 2-ketoisovaleric acid (see paper)
32% identity, 99% coverage: 3:466/469 of query aligns to 5:455/455 of 8jdxA
- binding flavin-adenine dinucleotide: E32 (= E30), P68 (= P66), G70 (= G68), T71 (≠ G69), G72 (≠ N70), T73 (= T71), G74 (= G72), G78 (= G76), V79 (≠ G77), L90 (≠ T90), P132 (≠ L132), G133 (= G133), A134 (= A134), G140 (= G142), M141 (≠ N143), A143 (≠ S145), T144 (= T146), A146 (= A148), S147 (≠ G149), E200 (= E199), G201 (= G200), I206 (≠ V205), W322 (≠ I331), E413 (= E424), H414 (= H425), N450 (= N461)
- binding 3-methyl-2-oxobutanoic acid: R318 (= R328), H369 (= H378), H376 (= H385), H414 (= H425)
- binding manganese (ii) ion: H369 (= H378), H376 (= H385), E413 (= E424)
8jdsA Crystal structure of mldhd in complex with pyruvate (see paper)
32% identity, 99% coverage: 3:466/469 of query aligns to 5:456/456 of 8jdsA
- binding flavin-adenine dinucleotide: E32 (= E30), P68 (= P66), G70 (= G68), T71 (≠ G69), G72 (≠ N70), T73 (= T71), G74 (= G72), G78 (= G76), V79 (≠ G77), L90 (≠ T90), P132 (≠ L132), G133 (= G133), A134 (= A134), G140 (= G142), M141 (≠ N143), A143 (≠ S145), T144 (= T146), A146 (= A148), S147 (≠ G149), E200 (= E199), G201 (= G200), I206 (≠ V205), W323 (≠ I331), E414 (= E424), H415 (= H425), N451 (= N461)
- binding manganese (ii) ion: H370 (= H378), H377 (= H385), E414 (= E424)
- binding pyruvic acid: R319 (= R328), H370 (= H378), H377 (= H385), H415 (= H425)
8jdvA Crystal structure of mldhd in complex with 2-ketohexanoic acid (see paper)
31% identity, 99% coverage: 3:466/469 of query aligns to 5:454/454 of 8jdvA
- binding 2-Ketohexanoic acid: V75 (≠ L73), R317 (= R321), W321 (= W325), H368 (= H378), H375 (= H385), H413 (= H425)
- binding flavin-adenine dinucleotide: P68 (= P66), G70 (= G68), T71 (≠ G69), G72 (≠ N70), T73 (= T71), G74 (= G72), G78 (= G76), V79 (≠ G77), L90 (≠ T90), P132 (≠ L132), G133 (= G133), A134 (= A134), G140 (= G142), M141 (≠ N143), A143 (≠ S145), T144 (= T146), A146 (= A148), S147 (≠ G149), E200 (= E199), G201 (= G200), I206 (≠ V205), W321 (= W325), Y322 (≠ R326), E412 (= E424), H413 (= H425), N449 (= N461)
- binding manganese (ii) ion: H368 (= H378), H375 (= H385), E412 (= E424)
8jdtA Crystal structure of mldhd in complex with 2-ketobutanoic acid (see paper)
31% identity, 99% coverage: 3:466/469 of query aligns to 5:455/455 of 8jdtA
- binding 2-ketobutyric acid: R318 (= R321), H369 (= H378), H376 (= H385), H414 (= H425)
- binding flavin-adenine dinucleotide: P68 (= P66), G70 (= G68), T71 (≠ G69), G72 (≠ N70), T73 (= T71), G74 (= G72), G78 (= G76), V79 (≠ G77), L90 (≠ T90), P132 (≠ L132), G133 (= G133), A134 (= A134), G140 (= G142), M141 (≠ N143), A143 (≠ S145), T144 (= T146), A146 (= A148), S147 (≠ G149), E200 (= E199), G201 (= G200), I206 (≠ V205), W322 (= W325), E413 (= E424), H414 (= H425), N450 (= N461)
- binding manganese (ii) ion: H369 (= H378), H376 (= H385), E413 (= E424)
8jduA Crystal structure of mldhd in complex with 2-ketovaleric acid (see paper)
31% identity, 99% coverage: 3:466/469 of query aligns to 5:455/455 of 8jduA
- binding 2-oxopentanoic acid: R318 (= R328), W322 (≠ I331), H369 (= H378), H376 (= H385), H414 (= H425)
- binding flavin-adenine dinucleotide: P68 (= P66), G70 (= G68), T71 (≠ G69), G72 (≠ N70), T73 (= T71), G74 (= G72), G78 (= G76), V79 (≠ G77), L90 (≠ T90), P132 (≠ L132), G133 (= G133), A134 (= A134), G140 (= G142), M141 (≠ N143), A143 (≠ S145), T144 (= T146), A146 (= A148), S147 (≠ G149), E200 (= E199), G201 (= G200), I206 (≠ V205), W322 (≠ I331), E413 (= E424), N450 (= N461)
- binding manganese (ii) ion: H369 (= H378), H376 (= H385), E413 (= E424)
8jdrA Crystal structure of h405a mldhd in complex with d-2-hydroxy-3-methyl- valeric acid (see paper)
31% identity, 99% coverage: 3:466/469 of query aligns to 5:456/456 of 8jdrA
- binding flavin-adenine dinucleotide: P68 (= P66), G70 (= G68), T71 (≠ G69), G72 (≠ N70), T73 (= T71), G74 (= G72), G78 (= G76), V79 (≠ G77), L90 (≠ T90), P132 (≠ L132), G133 (= G133), A134 (= A134), G140 (= G142), M141 (≠ N143), A143 (≠ S145), T144 (= T146), A146 (= A148), S147 (≠ G149), E200 (= E199), G201 (= G200), I206 (≠ V205), Y324 (≠ P332), H370 (= H378), E414 (= E424), N451 (= N461)
- binding (2R,3S)-3-methyl-2-oxidanyl-pentanoic acid: R319 (= R328), W323 (≠ I331), H415 (= H425)
8jdqA Crystal structure of h405a mldhd in complex with d-2-hydroxyisocaproic acid (see paper)
31% identity, 99% coverage: 3:466/469 of query aligns to 5:456/456 of 8jdqA
- binding (2R)-2-hydroxy-4-methylpentanoic acid: R319 (= R328), W323 (≠ I331), H370 (= H378), H415 (= H425)
- binding flavin-adenine dinucleotide: P68 (= P66), G70 (= G68), T71 (≠ G69), G72 (≠ N70), T73 (= T71), G74 (= G72), G78 (= G76), V79 (≠ G77), L90 (≠ T90), P132 (≠ L132), G133 (= G133), A134 (= A134), G140 (= G142), M141 (≠ N143), A143 (≠ S145), T144 (= T146), A146 (= A148), S147 (≠ G149), E200 (= E199), G201 (= G200), I206 (≠ V205), H370 (= H378), E414 (= E424), N451 (= N461)
Query Sequence
>WP_043745229.1 NCBI__GCF_000009985.1:WP_043745229.1
MSILHALSAIVGPGNVITEASDLAPYLVEERGLYQGAALGVVRPGSTAEVAEVVRLCAAA
GIPMVPQGGNTGLCGGGVPSEDGQSVVISTERLTRIRAVDPVDFTLTAEAGCVLANLQQA
AEEAGCLFPLSLGAEGSCRIGGNISTNAGGTNVLRYGNTRDLVLGLEVVLPDGRVWNGLK
RLRKDNTGYALQHLFIGAEGTLGIVTACVLKLFPRPREIATAFVALADLEAALPLFSRAR
TASGDSVTACELVPRRGLELGMRHVPGVRAPFAAFHDWMLLLELSSSRPGGLREALEEML
GQAFEDGLAVDAVIAESDAQRADFWRIREAIPEAQKKEGGSIKHDVAVATSRVPEMIRRC
TRAVEAAMPGVRVVPFGHLGDGNTHFNLTQPEGADKAAFLARWEEMNRIVHDIVVEMEGS
ISAEHGIGRLKVDELAHYKPGVDLDLMARIKGALDPLGQMNPGKILRRP
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory