SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_043767718.1 NCBI__GCF_000733765.1:WP_043767718.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P00325 All-trans-retinol dehydrogenase [NAD(+)] ADH1B; Alcohol dehydrogenase 1B; Alcohol dehydrogenase subunit beta; EC 1.1.1.105 from Homo sapiens (Human) (see 9 papers)
37% identity, 98% coverage: 5:377/379 of query aligns to 3:375/375 of P00325

query
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P00325

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H48 (= H51) to R: in dbSNP:rs1229984
N57 (≠ S60) to K: in dbSNP:rs1041969
T60 (≠ R63) to S: in dbSNP:rs6413413
R370 (= R372) to C: in beta-3/Indianapolis; allele ADH1B*3; decreased NAD(H) binding; dbSNP:rs2066702

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylserine

P25405 Alcohol dehydrogenase 1A; Alcohol dehydrogenase I-A; ADH IA; EC 1.1.1.1 from Saara hardwickii (Indian spiny-tailed lizard) (Uromastyx hardwickii) (see paper)
38% identity, 98% coverage: 5:377/379 of query aligns to 2:375/375 of P25405

query
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P25405

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Sites not aligning to the query:

1 modified: N-acetylglycine

1u3wA Crystal structure of human alcohol dehydrogenase gamma-2-gamma-2 isoform complexed with n-1-methylheptylformamide determined to 1.45 angstrom resolution (see paper)
37% identity, 98% coverage: 5:377/379 of query aligns to 2:374/374 of 1u3wA

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active site: C46 (= C50), R47 (≠ H51), S48 (= S52), H51 (≠ S55), H67 (= H71), E68 (= E72), C97 (= C101), C100 (= C104), C103 (= C107), C111 (= C115), D115 (≠ A119), C174 (= C179), T178 (= T183), R369 (= R372)
binding 1-methylheptylformamide: S48 (= S52), H67 (= H71), F93 (= F97), C174 (= C179)
binding nicotinamide-adenine-dinucleotide: C46 (= C50), R47 (≠ H51), S48 (= S52), H51 (≠ S55), C174 (= C179), T178 (= T183), G199 (= G204), G201 (= G206), V203 (= V208), D223 (= D228), I224 (≠ P229), K228 (= K233), V268 (≠ T271), I269 (≠ V272), T274 (≠ V277), V292 (= V294), G293 (= G295), V294 (≠ L296), I318 (≠ Y321)
binding zinc ion: C46 (= C50), H67 (= H71), C97 (= C101), C100 (= C104), C103 (= C107), C111 (= C115), C174 (= C179)

1u3vA Crystal structure of human alcohol dehydrogenase beta-1-beta-1 isoform complexed with n-heptylformamide determined to 1.65 angstrom resolution (see paper)
37% identity, 98% coverage: 5:377/379 of query aligns to 2:374/374 of 1u3vA

query
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1u3vA

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F

V

T

P

P

A

Q

C
|
C

G

G

T

K

C
|
C

S

R

Y

V

C
|
C

A

K

E

N

G

P

R

E

P

S

V

N

L

Y

C
|
C

V

L

P

K

G

N

A
x
D

A
x
L

A

G

N

N

S

P

A

R

G

G

T

T

L

L

L

Q

S

D

G

G

A

T

R

R

W

F

R

T

D

-

S

C

D

R

G

G

A

K

E

P

C

I

H

H

H

F

L

L

G

G

V

T

S

S

A

T

F

F

A

S

E

Q

R

Y

T

T

V

V

S

D

T

E

R

N

S

A

A

V

V

A

R

K

I

I

D

D

P

A

D

A

L

S

P

P

V

L

E

E

I

K

A

V

A

C

L

L

F

I

G

G

C
|
C

A

G

V

F

M

S

T
|
T

G

G

V

Y

G

G

A

S

A

A

V

V

N

N

T

V

A

A

A

K

V

V

R

T

P

P

G

G

E

S

S

T

V

C

A

A

V

V

F

F

G
|
G

M
x
L

G
|
G

G

G

V
|
V

G

G

L

L

A

S

A

A

L

V

L

M

G

G

A

C

I

K

A

A

G

G

A

A

W

A

P

R

V

I

A

A

V

V

D
|
D

P
x
I

V

N

A

K

A

D

K

K

R

F

E

A

L

K

A

A

L

K

S

E

L

L

G

G

A

A

T

T

H

E

V

C

V

I

D

N

P

P

T

Q

-

D

-

Y

A

K

E

K

D

P

A

I

V

Q

E

E

A

V

V

L

R

K

A

E

H

M

T

T

G

D

G

G

A

V

A

D

H

F

A

S

V

F

E

E

T
x
V

V
x
I

G

G

S
x
R

E

L

K

D

V

T

L

M

E

M

Q

A

A

S

Y

L

F

L

A

C

T

C

V

H

R

E

G

A

-

C

G

G

Q

T

T

S

V

V

T

I

V
|
V

G
|
G

L
x
V

P

P

H

P

P

A

E

S

R

Q

M

N

L

L

S

S

I

I

P

N

A

P

V

M

S

-

L

L

V

L

A

L

E

T

E

G

R

R

T

T

L

W

R

K

G

G

S

A

Y
x
V

M

Y

G

G

S

G

A

F

V

K

V

S

S

K

R

E

D

G

V

I

P

P

R

K

F

L

L

V

S

A

L

D

Y

F

R

M

A

A

G

K

R

K

L

F

P

S

V

L

D

D

K

A

L

L

L

I

T

T

H

H

E

V

L

L

A

P

L

F

D

E

G

K

I

I

N

N

A

E

A

G

F

F

D

D

R

L

L

L

A

H

R

S

A

G

E

K

A

S

V

I

R
|
R

Q

T

V

V

I

L

R

T

F

F

active site: C46 (= C50), R47 (≠ H51), T48 (≠ S52), H51 (≠ S55), H67 (= H71), E68 (= E72), C97 (= C101), C100 (= C104), C103 (= C107), C111 (= C115), D115 (≠ A119), C174 (= C179), T178 (= T183), R369 (= R372)
binding heptylformamide: T48 (≠ S52), H67 (= H71), F93 (= F97), L116 (≠ A120), C174 (= C179)
binding nicotinamide-adenine-dinucleotide: R47 (≠ H51), T48 (≠ S52), H51 (≠ S55), C174 (= C179), T178 (= T183), G199 (= G204), L200 (≠ M205), G201 (= G206), V203 (= V208), D223 (= D228), I224 (≠ P229), V268 (≠ T271), I269 (≠ V272), R271 (≠ S274), V292 (= V294), G293 (= G295), V294 (≠ L296), V318 (≠ Y321)
binding zinc ion: C46 (= C50), H67 (= H71), C97 (= C101), C100 (= C104), C103 (= C107), C111 (= C115), C174 (= C179)

1u3uA Crystal structure of human alcohol dehydrogenase beta-1-beta-1 isoform complexed with n-benzylformamide determined to 1.6 angstrom resolution (see paper)
37% identity, 98% coverage: 5:377/379 of query aligns to 2:374/374 of 1u3uA

query
sites
1u3uA

T

T

R

A

G

G

A

K

V

V

L

I

R

K

E

-

M

C

G

K

A

A

Q

V

P

L

Y

W

A

E

E

V

T

K

R

K

P

P

L

F

K

S

I

I

E

E

T

D

L

V

E

E

L

V

A

A

P

P

G

K

P

A

G

Y

E

E

I

V

Q

R

V

I

R

K

V

M

R

V

A

A

A

V

G

G

I

I

C
|
C

H
x
R

S
x
T

D

D

L

D

S
x
H

V

V

N

S

G

G

S

N

R

L

P

V

R

T

P

P

M

L

P

P

M

V

V

I

L

L

G

G

H
|
H

E
|
E

A

A

G

G

D

I

V

V

E

E

A

S

V

V

G

G

P

E

D

G

V

V

A

T

D

T

F

V

A

K

P

P

G

G

D

D

R

K

V

V

V

I

F

P

S

L

F
|
F

V

T

P

P

A

Q

C
|
C

G

G

T

K

C
|
C

S

R

Y

V

C
|
C

A

K

E

N

G

P

R

E

P

S

V

N

L

Y

C
|
C

V

L

P

K

G

N

A
x
D

A

L

A

G

N

N

S

P

A

R

G

G

T

T

L

L

L

Q

S

D

G

G

A

T

R

R

W

F

R

T

D

-

S

C

D

R

G

G

A

K

E

P

C

I

H

H

H

F

L

L

G

G

V

T

S

S

A

T

F

F

A

S

E

Q

R

Y

T

T

V

V

S

D

T

E

R

N

S

A

A

V

V

A

R

K

I

I

D

D

P

A

D

A

L

S

P

P

V

L

E

E

I

K

A

V

A

C

L

L

F

I

G

G

C
|
C

A

G

V

F

M

S

T
|
T

G

G

V

Y

G

G

A

S

A

A

V

V

N

N

T

V

A

A

A

K

V

V

R

T

P

P

G

G

E

S

S

T

V

C

A

A

V

V

F

F

G
|
G

M
x
L

G
|
G

G

G

V
|
V

G

G

L

L

A

S

A

A

L

V

L

M

G

G

A

C

I

K

A

A

G

G

A

A

W

A

P

R

V

I

A

A

V

V

D
|
D

P
x
I

V

N

A

K

A

D

K
|
K

R

F

E

A

L

K

A

A

L

K

S

E

L

L

G

G

A

A

T

T

H

E

V

C

V

I

D

N

P

P

T

Q

-

D

-

Y

A

K

E

K

D

P

A

I

V

Q

E

E

A

V

V

L

R

K

A

E

H

M

T

T

G

D

G

G

A

V

A

D

H

F

A

S

V

F

E

E

T
x
V

V
x
I

G

G

S
x
R

E

L

K

D

V

T

L

M

E

M

Q

A

A

S

Y

L

F

L

A

C

T

C

V

H

R

E

G

A

-

C

G

G

Q

T

T

S

V

V

T

I

V
|
V

G
|
G

L
x
V

P

P

H

P

P

A

E

S

R

Q

M

N

L

L

S

S

I

I

P

N

A

P

V

M

S

-

L

L

V

L

A

L

E

T

E

G

R

R

T

T

L

W

R

K

G

G

S

A

Y
x
V

M

Y

G

G

S

G

A

F

V

K

V

S

S

K

R

E

D

G

V

I

P

P

R

K

F

L

L

V

S

A

L

D

Y

F

R

M

A

A

G

K

R

K

L

F

P

S

V

L

D

D

K

A

L

L

L

I

T

T

H

H

E

V

L

L

A

P

L

F

D

E

G

K

I

I

N

N

A

E

A

G

F

F

D

D

R

L

L

L

A

H

R

S

A

G

E

K

A

S

V

I

R
|
R

Q

T

V

V

I

L

R

T

F

F

active site: C46 (= C50), R47 (≠ H51), T48 (≠ S52), H51 (≠ S55), H67 (= H71), E68 (= E72), C97 (= C101), C100 (= C104), C103 (= C107), C111 (= C115), D115 (≠ A119), C174 (= C179), T178 (= T183), R369 (= R372)
binding n-benzylformamide: T48 (≠ S52), H67 (= H71), F93 (= F97), C174 (= C179), V294 (≠ L296)
binding nicotinamide-adenine-dinucleotide: R47 (≠ H51), T48 (≠ S52), H51 (≠ S55), C174 (= C179), T178 (= T183), G199 (= G204), L200 (≠ M205), G201 (= G206), V203 (= V208), D223 (= D228), I224 (≠ P229), K228 (= K233), V268 (≠ T271), I269 (≠ V272), R271 (≠ S274), V292 (= V294), G293 (= G295), V294 (≠ L296), V318 (≠ Y321), R369 (= R372)
binding zinc ion: C46 (= C50), H67 (= H71), C97 (= C101), C100 (= C104), C103 (= C107), C111 (= C115), C174 (= C179)

1hdxB Three-dimensional structures of three human alcohol dehydrogenase variants: correlations with their functional differences (see paper)
37% identity, 98% coverage: 5:377/379 of query aligns to 2:374/374 of 1hdxB

query
sites
1hdxB

T

T

R

A

G

G

A

K

V

V

L

I

R

K

E

-

M

C

G

K

A

A

Q

V

P

L

Y

W

A

E

E

V

T

K

R

K

P

P

L

F

K

S

I

I

E

E

T

D

L

V

E

E

L

V

A

A

P

P

G

K

P

A

G

Y

E

E

I

V

Q

R

V

I

R

K

V

M

R

V

A

A

A

V

G

G

I

I

C
|
C

H
x
R

S
x
T

D

D

L

D

S
x
H

V

V

N

S

G

G

S

N

R

L

P

V

R

T

P

P

M

L

P

P

M

V

V

I

L

L

G

G

H
|
H

E
|
E

A

A

G

G

D

I

V

V

E

E

A

S

V

V

G

G

P

E

D

G

V

V

A

T

D

T

F

V

A

K

P

P

G

G

D

D

R

K

V

V

V

I

F

P

S

L

F

F

V

T

P

P

A

Q

C
|
C

G

G

T

K

C
|
C

S

R

Y

V

C
|
C

A

K

E

N

G

P

R

E

P

S

V

N

L

Y

C
|
C

V

L

P

K

G

N

A
x
D

A
x
L

A

G

N

N

S

P

A

R

G

G

T

T

L

L

L

Q

S

D

G

G

A

T

R

R

W

F

R

T

D

-

S

C

D

R

G

G

A

K

E

P

C

I

H

H

H

F

L

L

G

G

V

T

S

S

A

T

F

F

A

S

E

Q

R

Y

T

T

V

V

S

D

T

E

R

N

S

A

A

V

V

A

R

K

I

I

D

D

P

A

D

A

L

S

P

P

V

L

E

E

I

K

A

V

A

C

L

L

F

I

G

G

C
|
C

A

G

V

F

M

S

T
|
T

G

G

V

Y

G

G

A

S

A

A

V

V

N

N

T

V

A

A

A

K

V

V

R

T

P

P

G

G

E

S

S

T

V

C

A

A

V

V

F

F

G

G

M
x
L

G
|
G

V
|
V

G

G

L

L

A

S

A

A

L

V

L

M

G

G

A

C

I

K

A

A

G

G

A

A

W

A

P

R

V

I

A

A

V

V

D
|
D

P

I

V

N

A

K

A

D

K

K

R

F

E

A

L

K

A

A

L

K

S

E

L

L

G

G

A

A

T

T

H

E

V

C

V

I

D

N

P

P

T

Q

-

D

-

Y

A

K

E

K

D

P

A

I

V

Q

E

E

A

V

V

L

R

K

A

E

H

M

T

T

G

D

G

G

A

V

A

D

H

F

A

S

V

F

E

E

T

V

V
x
I

G

G

S
x
R

E

L

K

D

V

T

L

M

E

M

Q

A

A

S

Y

L

F

L

A

C

T

C

V

H

R

E

G

A

-

C

G

G

Q

T

T

S

V

V

T

I

V
|
V

G
|
G

L
x
V

P

P

H

P

P

A

E

S

R

Q

M

N

L

L

S

S

I

I

P

N

A

P

V

M

S

-

L

L

V

L

A

L

E

T

E

G

R

R

T

T

L

W

R

K

G

G

S

A

Y
x
V

M

Y

G

G

S

G

A

F

V

K

V

S

S

K

R

E

D

G

V

I

P

P

R

K

F

L

L

V

S

A

L

D

Y

F

R

M

A

A

G

K

R

K

L

F

P

S

V

L

D

D

K

A

L

L

L

I

T

T

H

H

E

V

L

L

A

P

L

F

D

E

G

K

I

I

N

N

A

E

A

G

F

F

D

D

R

L

L

L

A

H

R

S

A

G

E

K

A

S

V

I

R
|
R

Q

T

V

V

I

L

R

T

F

F

active site: C46 (= C50), R47 (≠ H51), T48 (≠ S52), H51 (≠ S55), H67 (= H71), E68 (= E72), C97 (= C101), C100 (= C104), C103 (= C107), C111 (= C115), D115 (≠ A119), C174 (= C179), T178 (= T183), R369 (= R372)
binding cyclohexanol: L116 (≠ A120), V318 (≠ Y321)
binding nicotinamide-adenine-dinucleotide: C46 (= C50), R47 (≠ H51), H51 (≠ S55), T178 (= T183), L200 (≠ M205), G201 (= G206), G202 (= G207), V203 (= V208), D223 (= D228), I269 (≠ V272), R271 (≠ S274), V292 (= V294), G293 (= G295), V294 (≠ L296), R369 (= R372)
binding zinc ion: C46 (= C50), H67 (= H71), C97 (= C101), C100 (= C104), C103 (= C107), C111 (= C115), C174 (= C179)

1dehA Crystallization of human beta1 alcohol dehydrogenase (15 mg/ml) in 50 mm sodium phosphate (ph 7.5), 2.0 mm NAD+ and 1 mm 4-iodopyrazole at 25 oc, 13% (w/v) peg 8000 (see paper)
37% identity, 98% coverage: 5:377/379 of query aligns to 2:374/374 of 1dehA

query
sites
1dehA

T

T

R

A

G

G

A

K

V

V

L

I

R

K

E

-

M

C

G

K

A

A

Q

V

P

L

Y

W

A

E

E

V

T

K

R

K

P

P

L

F

K

S

I

I

E

E

T

D

L

V

E

E

L

V

A

A

P

P

G

K

P

A

G

Y

E

E

I

V

Q

R

V

I

R

K

V

M

R

V

A

A

A

V

G

G

I

I

C
|
C

H
x
R

S
x
T

D

D

L

D

S
x
H

V

V

N

S

G

G

S

N

R

L

P

V

R

T

P

P

M

L

P

P

M

V

V

I

L

L

G

G

H
|
H

E
|
E

A

A

G

G

D

I

V

V

E

E

A

S

V

V

G

G

P

E

D

G

V

V

A

T

D

T

F

V

A

K

P

P

G

G

D

D

R

K

V

V

V

I

F

P

S

L

F
|
F

V

T

P

P

A

Q

C
|
C

G

G

T

K

C
|
C

S

R

Y

V

C
|
C

A

K

E

N

G

P

R

E

P

S

V

N

L

Y

C
|
C

V

L

P

K

G

N

A
x
D

A
x
L

A

G

N

N

S

P

A

R

G

G

T

T

L

L

L

Q

S

D

G

G

A

T

R

R

W

F

R

T

D

-

S

C

D

R

G

G

A

K

E

P

C

I

H

H

H

F

L

L

G

G

V

T

S

S

A

T

F

F

A

S

E

Q

R

Y

T

T

V

V

S

D

T

E

R

N

S

A

A

V

V

A

R

K

I

I

D

D

P

A

D

A

L

S

P

P

V

L

E

E

I

K

A

V

A

C

L

L

F

I

G

G

C
|
C

A

G

V

F

M

S

T
|
T

G

G

V

Y

G

G

A

S

A

A

V

V

N

N

T

V

A

A

A

K

V

V

R

T

P

P

G

G

E

S

S

T

V

C

A

A

V

V

F

F

G

G

M

L

G
|
G

G

G

V
|
V

G

G

L

L

A

S

A

A

L

V

L

M

G

G

A

C

I

K

A

A

G

G

A

A

W

A

P

R

V

I

A

A

V

V

D
|
D

P
x
I

V

N

A

K

A

D

K

K

R

F

E

A

L

K

A

A

L

K

S

E

L

L

G

G

A

A

T

T

H

E

V

C

V

I

D

N

P

P

T

Q

-

D

-

Y

A

K

E

K

D

P

A

I

V

Q

E

E

A

V

V

L

R

K

A

E

H

M

T

T

G

D

G

G

A

V

A

D

H

F

A

S

V

F

E

E

T
x
V

V
x
I

G

G

S

R

E

L

K

D

V

T

L

M

E

M

Q

A

A

S

Y

L

F

L

A

C

T

C

V

H

R

E

G

A

-

C

G

G

Q

T

T

S

V

V

T

I

V
|
V

G

G

L
x
V

P

P

H

P

P

A

E

S

R

Q

M

N

L

L

S

S

I

I

P

N

A

P

V

M

S

-

L

L

V

L

A

L

E

T

E

G

R

R

T

T

L

W

R

K

G

G

S

A

Y

V

M

Y

G

G

S

G

A

F

V

K

V

S

S

K

R

E

D

G

V

I

P

P

R

K

F

L

L

V

S

A

L

D

Y

F

R

M

A

A

G

K

R

K

L

F

P

S

V

L

D

D

K

A

L

L

L

I

T

T

H

H

E

V

L

L

A

P

L

F

D

E

G

K

I

I

N

N

A

E

A

G

F

F

D

D

R

L

L

L

A

H

R

S

A

G

E

K

A

S

V

I

R
|
R

Q

T

V

V

I

L

R

T

F

F

active site: C46 (= C50), R47 (≠ H51), T48 (≠ S52), H51 (≠ S55), H67 (= H71), E68 (= E72), C97 (= C101), C100 (= C104), C103 (= C107), C111 (= C115), D115 (≠ A119), C174 (= C179), T178 (= T183), R369 (= R372)
binding nicotinamide-adenine-dinucleotide: C46 (= C50), R47 (≠ H51), T48 (≠ S52), H51 (≠ S55), C174 (= C179), T178 (= T183), G201 (= G206), V203 (= V208), D223 (= D228), I224 (≠ P229), V268 (≠ T271), I269 (≠ V272), V292 (= V294), V294 (≠ L296)
binding 4-iodopyrazole: T48 (≠ S52), H67 (= H71), F93 (= F97), L116 (≠ A120), C174 (= C179)
binding zinc ion: C46 (= C50), H67 (= H71), C97 (= C101), C100 (= C104), C103 (= C107), C111 (= C115)

P00326 Alcohol dehydrogenase 1C; Alcohol dehydrogenase subunit gamma; EC 1.1.1.1 from Homo sapiens (Human) (see 5 papers)
37% identity, 98% coverage: 5:377/379 of query aligns to 3:375/375 of P00326

query
sites
P00326

T

T

R

A

G

G

A

K

V

V

L

I

R

K

E

-

M

C

G

K

A

A

Q

V

P

L

Y

W

A

E

E

L

T

K

R

K

P

P

L

F

K

S

I

I

E

E

T

E

L

V

E

E

L

V

A

A

P

P

G

K

P

A

G

H

E

E

I

V

Q

R

V

I

R

K

V

M

R

V

A

A

G

G

I

I

C
|
C

H
x
R

S

S

D

D

L

E

S

H

V

V

N

S

G

G

S

N

R

L

P

V

R

T

P

P

M

L

P

P

M

V

V

I

L

L

G

G

H
|
H

E

E

A

A

G

G

D

I

V

V

E

E

A

S

V

V

G

G

P

E

D

G

V

V

A

T

D

T

F

V

A

K

P

P

G

G

D

D

R

K

V

V

V

I

F

P

S

L

F

F

V

T

P

P

A

Q

C
|
C

G

G

T

K

C
|
C

S

R

Y

I

C
|
C

A

K

E

N

G

P

R

E

P

S

V

N

L

Y

C
|
C

V

L

P

K

G

N

A

D

A

L

A

G

N

N

S

P

A

R

G

G

T

T

L

L

L

Q

S

D

G

G

A

T

R

R

W

F

R

T

D

C

S

S

D

-

G

G

A

K

E

P

C

I

H

H

H

F

L

V

G

G

V

V

S

S

A

T

F

F

A

S

E

Q

R

Y

T

T

V

V

S

D

T

E

R

N

S

A

A

V

V

A

R

K

I

I

D

D

P

A

D

A

L

S

P
|
P

V

L

E

E

I

K

A

V

A

C

L

L

F

I

G

G

C
|
C

A

G

V

F

M

S

T

T

G

G

V

Y

G

G

A

S

A

A

V

V

N

K

T

V

A

A

A

K

V

V

R

T

P

P

G

G

E

S

S

T

V

C

A

A

V

V

F

F

G
|
G

M
x
L

G
|
G

V
|
V

G
|
G

L

L

A

S

A

V

L

V

L

M

G

G

A

C

I

K

A

A

G

G

A

A

W

A

P

R

V

I

A

A

V

V

D
|
D

P

I

V

N

A

K

A

D

K
|
K

R

F

E

A

L

K

A

A

L

K

S

E

L

L

G

G

A

A

T

T

H

E

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C

V

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Q

-

D

-

Y

A

K

E

K

D

P

A

I

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Q

E

E

A

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V

L

R

K

A

E

H

M

T

T

G

D

G

G

A

V

A

D

H

F

A

S

V

F

E

E

T

V

V

I

G

G

S
x
R

E

L

K

D

V

T

L

M

E

M

Q

A

A

S

Y

L

F

L

A

C

T

C

V

H

R

E

G

A

-

C

G

G

Q

T

T

S

V

V

T

I

V
|
V

G
|
G

L
x
V

P

P

H

P

P

D

E

S

R

Q

M

N

L

L

S

S

I

I

P

N

A

P

V

M

S

-

L

L

V

L

A

L

E

T

E

G

R

R

T

T

L

W

R

K

G

G

S
x
A

Y
x
I

M
x
F

G

G

S

G

A

F

V

K

V

S

S

K

R

E

D

S

V

V

P

P

R

K

F

L

L

V

S

A

L

D

Y

F

R

M

A

A

G

K

R

K

L

F

P

S

V

L

D

D

K

A

L

L

L

I

T

T

H

N

E
x
I

L

L

A
x
P

L

F

D

E

G

K

I

I

N

N

A

E

A

G

F

F

D

D

R

L

L

L

A

R

R

S

A

G

E

K

A

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|
R

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T

V

V

I

L

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T

F

F

C47 (= C50) binding Zn(2+)
R48 (≠ H51) to H: in dbSNP:rs35385902
H68 (= H71) binding Zn(2+)
C98 (= C101) binding Zn(2+)
C101 (= C104) binding Zn(2+)
C104 (= C107) binding Zn(2+)
C112 (= C115) binding Zn(2+)
P166 (= P170) to S: in dbSNP:rs34195308
C175 (= C179) binding Zn(2+)
GLGGVG 200:205 (≠ GMGGVG 204:209) binding NAD(+)
D224 (= D228) binding NAD(+)
K229 (= K233) binding NAD(+)
R272 (≠ S274) to Q: in allele ADH3*2/gamma-2; dbSNP:rs1693482
VGV 293:295 (≠ VGL 294:296) binding NAD(+)
AIF 318:320 (≠ SYM 320:322) binding NAD(+)
I350 (≠ E352) to V: in allele ADH3*2/gamma-2; dbSNP:rs698
P352 (≠ A354) to T: in dbSNP:rs35719513
R370 (= R372) binding NAD(+)

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylserine

P80512 Alcohol dehydrogenase 1; EC 1.1.1.1 from Naja naja (Indian cobra) (see paper)
37% identity, 98% coverage: 5:377/379 of query aligns to 2:375/375 of P80512

query
sites
P80512

T

T

R

A

G

G

A

K

V

V

L

I

R

K

E

-

M

C

G

K

A

A

Q

I

P

A

Y

W

A

E

E

L

T

N

R

K

P

P

L

L

K

S

I

I

E

E

T

Q

L

I

E

E

L

V

A

A

P

P

G

K

P

A

G

H

E

E

I

V

Q

R

V

I

R

K

V

I

R

L

A

A

A

T

G

G

I

V

C

C

H

R

S

S

D

D

L

E

S

H

V

V

V

I

N

S

G

G

S

A

R

F

P

R

R

M

P

P

M

L

P

P

M

M

V

V

L

L

G

G

H

H

E

E

A

A

G

G

D

V

V

V

E

E

A

S

V

V

G

G

P

E

D

G

V

V

A

T

D

C

F

V

A

K

P

P

G

G

D

D

R

K

V

V

V

I

F

P

S

L

F

F

V

A

P

P

A

Q

C

C

G

G

T

K

C

C

S

R

Y

A

C

C

A

Q

E

S

G

P

R

K

P

G

V

N

L

L

C

C

V

T

P

S

G

N

A

D

A

L

A

N

N

S

S

G

A

S

G

G

T

L

L

M

L

P

S

D

G

G

A

T

R

S

R

R

W

F

R

T

D

-

S

C

D

K

G

G

A

K

E

S

C

I

H

H

H

F

L

I

G

S

V

T

S

S

A

T

F

F

A

T

E

E

R

Y

T

T

V

V

S

H

T

E

R

N

S

S

A

V

V

V

R

K

I

I

D

D

P

P

D

S

L

A

P

P

V

L

E

E

I

K

A

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A

C

L

L

F

I

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G

C

C

A

G

V

F

M

S

T

T

G

G

V

Y

G

G

A

A

V

M

N

Q

T

T

A

A

A

K

V

V

R

E

P

P

G

G

E

S

S

I

V

C

A

A

V

V

F

F

G

G

M

L

G

G

V

V

G

G

L

L

A

S

A

V

L

V

L

M

G

G

A

C

I

K

A

A

G

G

A

A

W

S

P

R

V

I

A

G

V

V

D

D

P

I

V

N

A

K

A

D

K

K

R

F

E

A

L

K

A

A

L

K

S

E

L

M

G

G

A

A

T

T

H

E

V

C

V

I

D

N

P

P

T

L

-

D

-

F

A

K

E

K

D

P

A

I

V

N

E

E

A

V

V

L

R

F

A

D

H

L

T

T

G

G

-

E

G

G

A

V

A

D

H

Y

A

S

V

F

E

E

T

V

V

I

G

G

S

R

E

T

K

E

V

T

L

M

E

I

Q

S

A

A

Y

F

F

T

A

S

T

C

V

H

R

Q

G

N

-

L

G

G

Q

T

T

S

V

V

T

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V

V

G

G

L

V

P

P

H

P

P

N

E

A

R

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I

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I

Y

P

N

A

P

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L

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M

L

L

V

F

A

T

E

-

E

G

R

R

T

T

L

W

R

K

G

G

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C

Y

V

M

F

G

G

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G

A

W

V

K

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S

S

K

R

D

D

S

V

V

P

P

R

K

F

L

L

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S

S

L

D

Y

F

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A

Q

G

K

R

K

L

F

P

V

V

L

D

D

K

P

L

L

L

I

T

T

H

H

E

T

L

L

A

P

L

F

D

E

G

K

I

I

N

N

A

E

A

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F

F

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D

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L

L

L

A

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A

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E

K

A

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I

R

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Q

T

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V

I

L

R

I

F

F

Sites not aligning to the query:

1 modified: N-acetylserine

P80222 Alcohol dehydrogenase 1; Alcohol dehydrogenase, major; EC 1.1.1.1 from Alligator mississippiensis (American alligator) (see paper)
36% identity, 98% coverage: 5:377/379 of query aligns to 2:374/374 of P80222

query
sites
P80222

T

T

R

A

G

G

A

K

V

V

L

I

R

K

E

-

M

C

G

K

A

A

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A

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V

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A

A

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G

I

I

C

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D

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D

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H

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V

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N

A

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T

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P

P

M

L

P

P

M

M

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I

L

L

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H

E

E

A

A

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V

V

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E

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E

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A

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P

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D

R

K

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I

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F

F

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V

P

P

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Q

C

C

G

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E

C

C

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M

Y

P

C

C

A

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E

K

G

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R

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P

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N

L

L

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C

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A

D

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A

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A

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L

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F

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I

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F

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A

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A

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A

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C

C

A

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F

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T

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Y

G

G

A

A

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N

K

T

D

A

A

A

K

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P

G

G

E

S

S

T

V

C

A

A

V

V

F

F

G

G

M

L

G

G

V

V

G

G

L

L

A

S

A

T

L

I

L

M

G

G

A

C

I

K

A

A

G

G

A

A

W

S

P

R

V

I

A

G

V

I

D

D

P

I

V

N

A

K

A

D

K

K

R

F

E

A

L

K

A

A

L

K

S

E

L

L

G

G

A

A

T

T

H

E

V

C

V

I

D

N

P

P

-

L

-

D

T

C

A

K

E

K

D

P

A

I

V

Q

E

E

A

V

V

L

R

S

A

E

H

M

T

T

G

G

A

V

A

D

H

Y

A

S

V

F

E

E

T

V

V

I

G

G

S

R

E

I

K

D

V

T

L

M

E

T

Q

A

A

A

Y

L

F

A

A

C

T

C

V

Q

R

D

G

N

-

Y

G

G

Q

T

T

S

V

V

T

I

V

V

G

G

L

V

P

P

H

P

P

A

E

S

R

E

M

K

L

I

S

T

I

F

P

N

A

P

V

M

S

M

L

L

V

F

A

T

E

-

E

G

R

R

T

T

L

W

R

K

G

G

S

S

Y

V

M

F

G

G

S

G

A

W

V

K

V

S

S

K

R

E

D

S

V

V

P

P

R

K

F

L

L

V

S

A

L

D

Y

Y

R

M

A

E

G

K

R

K

L

I

P

N

V

L

D

D

K

G

L

L

L

I

T

T

H

H

E

T

L

L

A

P

L

F

D

D

G

K

I

I

N

N

A

E

A

G

F

F

D

E

R

L

L

L

A

R

R

T

A

G

E

K

A

S

V

I

R

R

Q

S

V

V

I

L

R

T

F

F

Sites not aligning to the query:

1 modified: N-acetylserine

Q17335 Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Alcohol dehydrogenase homolog 5; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Caenorhabditis elegans (see paper)
35% identity, 96% coverage: 15:378/379 of query aligns to 13:382/384 of Q17335

query
sites
Q17335

A

A

Q

V

P

A

Y

W

A

S

E

A

T

K

R

A

P

P

L

L

K

S

I

I

E

E

T

T

L

I

E

Q

L

V

A

A

P

P

G

K

P

A

G

H

E

E

I

V

Q

R

V

V

R

K

V

I

R

L

A

Y

A

T

G

A

I

V

C

C

H

H

S

T

D

D

L

A

S

Y

V

T

V

L

N

D

G

G

S

H

R

D

P

P

R

E

P

G

M

L

-

F

P

P

M

V

V

V

L

L

G

G

H

H

E

E

A

G

A

S

G

G

D

I

V

V

E

E

A

S

V

V

G

G

P

E

D

G

V

V

A

T

D

G

F

F

A

A

P

P

G

G

D

D

R

H

V

V

F

P

S

L

F

Y

V

V

P

P

A

Q

C

C

G

K

T

E

C

C

S

E

Y

Y

C

C

A

K

E

N

G

P

R

K

P

T

V

N

L

L

C

C

V

Q

P

K

G

I

A

R

A

I

A
x
S

N
x
Q

S
x
G

A
x
N

G
|
G

T
x
F

L
x
M

L
x
P

S
x
D

G
|
G

A
x
S

R
x
S

R
|
R

W
x
F

R
x
T

D

-

S
x
C

D
x
N

G
|
G

A
x
K

E
x
Q

C
x
L

H
x
F

H
|
H

H
x
F

L
x
M

G
|
G

V
x
C

S
|
S

A
x
T

F
|
F

A
x
S

E
|
E

R
x
Y

T
|
T

V
|
V

S
x
A

T
x
D

R
x
I

S
|
S

A
x
L

V
x
C

R
x
K

I
x
V

D
x
N

P
|
P

D
x
E

L
x
A

P
|
P

V
x
L

E
|
E

I
x
K

A
x
V

A
x
S

L
|
L

F
x
L

G
|
G

C
|
C

A
x
G

V
x
I

M
x
S

T
|
T

G
|
G

V
x
Y

G
|
G

A
|
A

A
x
V

V
x
L

N
|
N

T
|
T

A
x
C

A
x
K

V
|
V

R
x
E

P
x
E

G
|
G

E
x
S

S
x
T

V
|
V

A
|
A

V
|
V

F
x
W

G
|
G

M
x
L

G
|
G

G
x
A

V
|
V

G
|
G

L
|
L

A
|
A

A
x
V

L
x
I

L
x
M

G
|
G

A
|
A

I
x
K

A
|
A

G
|
G

A
|
A

W
x
K

P
x
K

V
x
I

V
|
V

A
x
G

V
x
I

D
|
D

P
x
L

V
x
I

A
x
E

A
x
S

K
|
K

R
x
F

E
|
E

L
x
S

A
|
A

L
x
K

S
x
F

L
x
F

G
|
G

A
|
A

T
|
T

H
x
E

V
x
C

V
x
I

D
x
N

P
|
P

T
x
K

A
x
S

E
x
V

D
x
E

A
x
L

V
x
P

E
|
E

A
x
G

-
x
K

-
x
S

-
x
F

-
x
Q

-
x
A

-
x
W

V
x
L

R
x
V

A
x
E

H
x
Q

T
x
F

G
x
D

G
|
G

A
x
F

A
x
D

H
x
Y

A
x
T

V
x
F

E
|
E

T
x
C

V
x
I

G
|
G

S
x
N

E
x
V

K
x
H

V
x
T

L
x
M

E
x
R

Q
|
Q

A
|
A

Y
x
L

F
x
E

A
|
A

T
x
A

V
x
H

R
x
K

G
|
G

-
x
W

G
|
G

Q
x
V

T
x
S

V
x
C

T
x
I

V
x
I

G
|
G

L
x
V

P
x
A

H
x
G

P
x
A

E
x
G

R
x
Q

M
x
E

L
x
I

S
x
A

I
x
T

P
x
R

A
x
P

V
x
F

S
x
Q

L
|
L

V
|
V

A
x
T

E

-

E
x
G

R
|
R

T
|
T

L
x
W

R
x
K

G
|
G

S
x
T

Y
x
A

M
x
F

G
|
G

S
x
G

A
x
W

V
x
K

V
x
S

S
x
V

R
x
E

D
x
S

V
|
V

P
|
P

R
|
R

F
x
L

L
x
V

S
x
D

L
x
D

Y
|
Y

R
x
M

A
x
N

G
x
K

R
x
K

L
|
L

P
x
L

V
x
I

D
|
D

K
x
E

L
x
F

L
x
I

T
|
T

H
|
H

E
x
R

L
x
W

A
x
N

L
x
I

D
|
D

G
x
D

I
|
I

N
|
N

A
x
T

A
|
A

F
|
F

D
|
D

R
x
V

L
|
L

A
x
H

R
x
K

A
x
G

E
|
E

A
x
S

V
x
L

R
|
R

Q
x
S

V
|
V

I
x
L

R
x
A

F
|
F

D
x
E

Sites not aligning to the query:

120:384 mutation Missing: In nu518; diminishes the calcium flux to the cytoplasm in the ASJ sensory neurons upon removal of a nitric oxide stimulus.

P07327 Alcohol dehydrogenase 1A; Alcohol dehydrogenase subunit alpha; EC 1.1.1.1 from Homo sapiens (Human) (see 3 papers)
36% identity, 98% coverage: 5:377/379 of query aligns to 3:375/375 of P07327

query
sites
P07327

T

T

R

A

G

G

A

K

V

V

L

I

R

K

E

-

M

C

G

K

A

A

Q

V

P

L

Y

W

A

E

E

L

T

K

R

K

P

P

L

F

K

S

I

I

E

E

T

E

L

V

E

E

L

V

A

A

P

P

G

K

P

A

G

H

E

E

I

V

Q

R

V

I

R

K

V

M

R

V

A

A

A

V

G

G

I

I

C
|
C

H
x
G

S
x
T

D
|
D

L
x
D

S
x
H

V

V

N

S

G

G

S

T

R

M

P

V

R

T

P

P

M

L

P

P

M

V

V

I

L

L

G

G

H
|
H

E

E

A

A

G

G

D

I

V

V

E

E

A

S

V

V

G

G

P

E

D

G

V

V

A

T

D

T

F

V

A

K

P

P

G

G

D

D

R

K

V

V

V

I

F

P

S

L

F

A

V

I

P

P

A

Q

C
|
C

G

G

T

K

C
|
C

S

R

Y

I

C
|
C

A

K

E

N

G

P

R

E

P

S

V

N

L

Y

C
|
C

V

L

P

K

G

N

A

D

A

V

A

S

N

N

S

P

A

Q

G

G

T

T

L

L

L

Q

S

D

G

G

A

T

R

S

R

R

W

F

R

T

D

-

S

C

D

R

G

R

A

K

E

P

C

I

H

H

H

F

L

L

G

G

V

I

S

S

A

T

F

F

A

S

E

Q

R

Y

T

T

V

V

S

D

T

E

R

N

S

A

A

V

V

A

R

K

I

I

D

D

P

A

D

A

L

S

P

P

V

L

E

E

I

K

A

V

A

C

L

L

F

I

G

G

C
|
C

A

G

V

F

M

S

T

T

G

G

V

Y

G

G

A

S

A

A

V

V

N

N

T

V

A

A

A

K

V

V

R

T

P

P

G

G

E

S

S

T

V

C

A

A

V

V

F

F

G
|
G

M
x
L

G
|
G

V
|
V

G
|
G

L

L

A

S

A

A

L

I

L

M

G

G

A

C

I

K

A

A

G

G

A

A

W

A

P

R

V

I

A

A

V

V

D
|
D

P

I

V

N

A

K

A

D

K
|
K

R

F

E

A

L

K

A

A

L

K

S

E

L

L

G

G

A

A

T

T

H

E

V

C

V

I

D

N

P

P

T

Q

-

D

-

Y

A

K

E

K

D

P

A

I

V

Q

E

E

A

V

V

L

R

K

A

E

H

M

T

T

G

D

G

G

A

V

A

D

H

F

A

S

V

F

E

E

T

V

V
x
I

G

G

S

R

E

L

K

D

V

T

L

M

E

M

Q

A

A

S

Y

L

F

L

A

C

T

C

V

H

R

E

G

A

-

C

G

G

Q

T

T

S

V

V

T

I

V
|
V

G
|
G

L
x
V

P

P

H

P

P

D

E

S

R

Q

M

N

L

L

S

S

I

M

P

N

A

P

V

M

S

-

L

L

V

L

A

L

E

T

E

G

R

R

T

T

L

W

R

K

G

G

S
x
A

Y
x
I

M
x
L

G

G

S

G

A

F

V

K

V

S

S

K

R

E

D

C

V

V

P

P

R

K

F

L

L

V

S

A

L

D

Y

F

R

M

A

A

G

K

R

K

L

F

P

S

V

L

D

D

K

A

L

L

L

I

T

T

H

H

E

V

L

L

A

P

L

F

D

E

G

K

I

I

N

N

A

E

A

G

F

F

D

D

R

L

L

L

A

H

R

S

A

G

E

K

A

S

V

I

R
|
R

Q

T

V

I

I

L

R

M

F

F

C47 (= C50) binding Zn(2+)
GTDDH 48:52 (≠ HSDLS 51:55) binding NAD(+)
H68 (= H71) binding Zn(2+)
C98 (= C101) binding Zn(2+)
C101 (= C104) binding Zn(2+)
C104 (= C107) binding Zn(2+)
C112 (= C115) binding Zn(2+)
C175 (= C179) binding Zn(2+)
GLGGVG 200:205 (≠ GMGGVG 204:209) binding NAD(+)
D224 (= D228) binding NAD(+)
K229 (= K233) binding NAD(+)
I270 (≠ V272) binding NAD(+)
VGV 293:295 (≠ VGL 294:296) binding NAD(+)
AIL 318:320 (≠ SYM 320:322) binding NAD(+)
R370 (= R372) binding NAD(+)

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylserine

1u3tA Crystal structure of human alcohol dehydrogenase alpha-alpha isoform complexed with n-cyclopentyl-n-cyclobutylformamide determined to 2.5 angstrom resolution (see paper)
36% identity, 98% coverage: 5:377/379 of query aligns to 2:374/374 of 1u3tA

query
sites
1u3tA

T

T

R

A

G

G

A

K

V

V

L

I

R

K

E

-

M

C

G

K

A

A

Q

V

P

L

Y

W

A

E

E

L

T

K

R

K

P

P

L

F

K

S

I

I

E

E

T

E

L

V

E

E

L

V

A

A

P

P

G

K

P

A

G

H

E

E

I

V

Q

R

V

I

R

K

V

M

R

V

A

A

A

V

G

G

I

I

C
|
C

H
x
G

S
x
T

D

D

L

D

S
x
H

V

V

N

S

G

G

S

T

R

M

P

V

R

T

P

P

M

L

P

P

M

V

V

I

L

L

G

G

H
|
H

E
|
E

A

A

G

G

D

I

V

V

E

E

A

S

V

V

G

G

P

E

D

G

V

V

A

T

D

T

F

V

A

K

P

P

G

G

D

D

R

K

V

V

V

I

F

P

S

L

F
x
A

V
x
I

P

P

A

Q

C
|
C

G

G

T

K

C
|
C

S

R

Y

I

C
|
C

A

K

E

N

G

P

R

E

P

S

V

N

L

Y

C
|
C

V

L

P

K

G

N

A
x
D

A

V

A

S

N

N

S

P

A

Q

G

G

T

T

L

L

L

Q

S

D

G

G

A

T

R

S

R

R

W

F

R

T

D

-

S

C

D

R

G

R

A

K

E

P

C

I

H

H

H

F

L

L

G

G

V

I

S

S

A

T

F

F

A

S

E

Q

R

Y

T

T

V

V

S

D

T

E

R

N

S

A

A

V

V

A

R

K

I

I

D

D

P

A

D

A

L

S

P

P

V

L

E

E

I

K

A

V

A

C

L

L

F

I

G

G

C
|
C

A

G

V

F

M

S

T
|
T

G

G

V

Y

G

G

A

S

A

A

V

V

N

N

T

V

A

A

A

K

V

V

R

T

P

P

G

G

E

S

S

T

V

C

A

A

V

V

F

F

G

G

M
x
L

G
|
G

G

G

V
|
V

G

G

L

L

A

S

A

A

L

I

L

M

G

G

A

C

I

K

A

A

G

G

A

A

W

A

P

R

V

I

A

A

V

V

D
|
D

P
x
I

V

N

A

K

A

D

K

K

R

F

E

A

L

K

A

A

L

K

S

E

L

L

G

G

A

A

T

T

H

E

V

C

V

I

D

N

P

P

T

Q

-

D

-

Y

A

K

E

K

D

P

A

I

V

Q

E

E

A

V

V

L

R

K

A

E

H

M

T

T

G

D

G

G

A

V

A

D

H

F

A

S

V

F

E

E

T

V

V
x
I

G

G

S

R

E

L

K

D

V

T

L

M

E

M

Q

A

A

S

Y

L

F

L

A

C

T

C

V

H

R

E

G

A

-

C

G

G

Q

T

T

S

V

V

T

I

V
|
V

G
|
G

L
x
V

P

P

H

P

P

D

E

S

R

Q

M

N

L

L

S

S

I

M

P

N

A

P

V

M

S

-

L

L

V

L

A

L

E

T

E

G

R

R

T

T

L

W

R

K

G

G

S

A

Y
x
I

M

L

G

G

S

G

A

F

V

K

V

S

S

K

R

E

D

C

V

V

P

P

R

K

F

L

L

V

S

A

L

D

Y

F

R

M

A

A

G

K

R

K

L

F

P

S

V

L

D

D

K

A

L

L

L

I

T

T

H

H

E

V

L

L

A

P

L

F

D

E

G

K

I

I

N

N

A

E

A

G

F

F

D

D

R

L

L

L

A

H

R

S

A

G

E

K

A

S

V

I

R
|
R

Q

T

V

I

I

L

R

M

F

F

active site: C46 (= C50), G47 (≠ H51), T48 (≠ S52), H51 (≠ S55), H67 (= H71), E68 (= E72), C97 (= C101), C100 (= C104), C103 (= C107), C111 (= C115), D115 (≠ A119), C174 (= C179), T178 (= T183), R369 (= R372)
binding cyclobutyl(cyclopentyl)formamide: T48 (≠ S52), H67 (= H71), A93 (≠ F97), I94 (≠ V98), I318 (≠ Y321)
binding nicotinamide-adenine-dinucleotide: C46 (= C50), T48 (≠ S52), H51 (≠ S55), C174 (= C179), T178 (= T183), L200 (≠ M205), G201 (= G206), V203 (= V208), D223 (= D228), I224 (≠ P229), I269 (≠ V272), V292 (= V294), G293 (= G295), V294 (≠ L296)
binding zinc ion: C46 (= C50), H67 (= H71), C97 (= C101), C100 (= C104), C103 (= C107), C111 (= C115), C174 (= C179)

P80468 All-trans-retinol dehydrogenase [NAD(+)] ADH4; Alcohol dehydrogenase 4; Alcohol dehydrogenase class II; EC 1.1.1.105 from Struthio camelus (Common ostrich) (see 2 papers)
35% identity, 98% coverage: 5:377/379 of query aligns to 2:379/379 of P80468

query
sites
P80468

T

T

R

E

G

G

A

K

V

V

L

I

R

K

E

-

M

C

G

K

A

A

Q

I

P

A

Y

W

A

E

E

A

T

G

R

K

P

P

L

L

K

S

I

V

E

E

T

E

L

I

E

E

L

V

A

S

P

P

G

K

P

D

G

H

E

E

I

V

Q

R

V

V

R

K

V

I

R

V

A

A

A

T

G

G

I

V

C

C

H

R

S

T

D

D

L

E

S

H

V

A

V

I

N

N

G

P

S

S

-

F

R

K

P

E

R

G

P

V

M

F

P

P

M

V

V

I

L

L

G

G

H

H

E

E

A

G

A

A

G

G

D

I

V

V

E

E

A

S

V

I

G

G

P

Q

D

G

V

V

A

S

D

K

F

F

A

K

P

P

G

G

D

D

R

K

V

V

V

I

F

P

S

L

F

Y

V

M

P

P

A

Q

C

C

G

G

T

H

C

C

S

K

Y

F

C

C

A

L

E

N

G

P

R

K

P

T

V

N

L

L

C

C

V

E

P

K

G

I

A

S

A

K

A

I

N

K

-

T

-

P

-

I

S

S

A

D

G

Q

T

E

L

V

L

M

S

S

G

D

A

G

R

T

R

S

W

R

R

F

D

T

S

C

D

K

G

G

A

K

E

P

C

I

H

Y

H

H

H

F

L

M

G

G

V

T

S

S

A

T

F

F

A

S

E

E

R

Y

T

T

V

V

S

S

T

E

R

S

S

S

A

L

V

A

R

K

I

I

D

D

P

A

D

A

L

A

P

P

V

L

E

D

I

K

A

V

A

C

L

L

F

I

G

G

C

C

A

G

V

F

M

S

T

T

G

G

V

Y

G

G

A

A

V

I

N

N

T

T

A

A

A

Q

V

V

R

E

P

P

G

G

E

S

S

T

V

C

A

A

V

V

F

F

G

G

M

L

G

G

V

V

G

G

L

L

A

S

A

A

L

V

L

M

G

G

A

C

I

K

A

A

G

G

A

A

W

S

P

K

V

I

V

F

A

G

V

I

D

D

P

I

V

N

A

K

A

D

K

K

R

F

E

P

L

L

A

A

L

K

S

K

L

L

G

G

A

A

T

T

H

D

V

C

V

L

D

N

P

P

T

Q

A

D

-

I

-

R

E

K

D

P

A

V

V

Q

E

E

A

I

V

I

R

A

A

E

H

M

T

T

G

N

G

G

A

V

A

D

H

F

A

A

V

I

E

E

T

C

V

I

G

G

S

N

E

P

K

D

V

V

L

M

E

K

Q

A

A

A

Y

F

F

E

A

S

T

T

V

T

R

V

G

G

-

W

G

G

Q

T

T

C

V

V

T

I

V

V

G

G

L

V

P

A

H

V

P

G

E

E

R

Q

M

S

L

I

S

P

I

F

P

S

A

P

V

M

S

Q

L

L

V

I

A

M

E

-

E

G

R

R

T

K

L

I

R

K

G

A

S

T

Y

F

M

F

G

G

S

G

A

W

V

K

V

S

S

V

R

K

D

S

V

V

P

P

R

K

F

L

L

V

S

S

L

D

Y

Y

R

M

A

A

G

K

R

K

L

F

P

D

V

L

D

D

K

A

L

L

L

V

T

S

H

H

E

T

L

L

A

P

L

L

D

D

G

K

I

I

N

N

A

D

A

A

F

F

D

D

R

L

L

M

A

N

R

A

A

G

E

K

A

S

V

N

R

R

Q

T

V

I

I

L

R

V

F

F

Sites not aligning to the query:

1 modified: N-acetylthreonine

P26325 Alcohol dehydrogenase 1; EC 1.1.1.1 from Gadus morhua subsp. callarias (Baltic cod) (Gadus callarias) (see paper)
36% identity, 99% coverage: 5:378/379 of query aligns to 2:375/375 of P26325

query
sites
P26325

T

T

R

V

G

G

A

K

V

V

L

I

R

K

E

-

M

C

G

K

A

A

Q

V

P

A

Y

W

A

E

E

A

T

N

R

K

P

P

L

L

K

V

I

I

E

E

T

E

L

I

E

E

L

V

A

D

P

V

P

P

G

H

P

A

G

N

E

E

I

I

Q

R

V

I

R

K

V

I

R

I

A

A

A

T

G

G

I

V

C

C

H

H

S

T

D

D

L

L

-

Y

S

H

V

L

V

F

N

E

G

G

S

K

R

H

P

K

R

D

P

G

M

F

P

P

M

V

V

V

L

L

G

G

H

H

E

E

A

G

A

A

G

G

D

I

V

V

E

E

A

S

V

V

G

G

P

P

D

G

V

V

A

T

D

E

F

F

A

Q

P

P

G

G

D

E

R

K

V

V

V

I

F

P

S

L

F

F

V

I

P

S

A

Q

C

C

G

G

T

E

C

C

S

R

Y

F

C

C

A

Q

E

S

G

P

R

K

P

T

V

N

L

Q

C

C

V

V

P

K

G

G

A

W

A

A

A

N

N

E

S

S

A

P

G

D

T

V

L

M

L

S

S

P

G

K

A

E

R

T

R

R

W

F

R

T

D

-

S

C

D

K

G

G

A

R

E

K

C

V

H

L

H

Q

H

F

L

L

G

G

V

T

S

S

A

T

F

F

A

S

E

Q

R

Y

T

T

V

V

S

N

T

Q

R

I

S

A

A

V

V

A

R

K

I

I

D

D

P

P

D

S

L

A

P

P

V

L

E

D

I

T

A

V

A

C

L

L

F

L

G

G

C

C

A

G

V

V

M

S

T

T

G

G

V

F

G

G

A

A

V

V

N

N

T

T

A

A

A

K

V

V

R

E

P

P

G

G

E

S

S

T

V

C

A

A

V

V

F

F

G

G

M

L

G

G

G

A

V

V

G

G

L

L

A

A

L

V

L

M

G

G

A

C

I

H

A

S

A

A

G

G

A

A

W

K

P

R

V

I

A

A

V

V

D

D

P

L

V

N

A

P

A

D

K

K

R

F

E

E

L

K

A

A

L

K

S

V

L

F

G

G

A

A

T

T

H

D

V

F

V

V

D

N

P

P

T

N

-

D

-

H

A

S

E

E

D

P

A

I

V

S

E

Q

A

V

V

L

R

S

A

K

H

M

T

T

G

N

G

G

A

V

A

D

H

F

A

S

V

L

E

E

T

C

V

V

G

G

S

N

E

V

K

G

V

V

L

M

E

R

Q

N

A

A

Y

L

F

E

A

S

T

C

V

L

R

K

G

G

-

W

G

G

Q

V

T

S

V

V

T

L

V

V

G

G

L

W

P

T

H

D

P

L

E

H

R

D

M

V

L

A

S

T

I

R

P

P

A

-

V

I

S

Q

L

L

V

I

A

A

E

-

E

G

R

R

T

T

L

W

R

K

G

G

S

S

Y

M

M

F

G

G

S

G

A

F

V

K

V

G

S

K

R

D

D

G

V

V

P

P

R

K

F

M

L

V

S

K

L

A

Y

Y

R

L

A

D

G

K

R

K

L

V

P

K

V

L

D

D

K

E

L

F

L

I

T

T

H

H

E

R

L

M

A

P

L

L

D

E

G

S

I

V

N

N

A

D

A

A

F

I

D

D

R

L

L

M

A

K

R

H

A

G

E

K

A

C

V

I

R

R

Q

T

V

V

I

L

R

S

F

L

D

E

Sites not aligning to the query:

1 modified: N-acetylalanine

P19631 Alcohol dehydrogenase 1; ADH3; Alcohol dehydrogenase subunit alpha; EC 1.1.1.1 from Coturnix japonica (Japanese quail) (Coturnix coturnix japonica) (see paper)
36% identity, 98% coverage: 5:375/379 of query aligns to 2:373/375 of P19631

query
sites
P19631

T

T

R

A

G

G

A

K

V

V

L

I

R

K

E

-

M

C

G

K

A

A

Q

V

P

L

Y

W

A

E

E

A

T

N

R

K

P

P

L

F

K

S

I

L

E

E

T

E

L

V

E

E

L

V

A

A

P

P

G

K

P

A

G

H

E

E

I

V

Q

R

V

I

R

K

V

I

R

V

A

A

A

T

G

G

I

I

C

C

H

R

S

S

D

D

L

D

S

H

V

V

N

T

G

G

S

A

R

L

P

A

R

M

P

P

M

F

P

P

M

V

V

I

L

L

G

G

H

H

E

E

A

A

G

G

D

V

V

V

E

E

A

S

V

V

G

G

P

E

D

K

V

V

A

T

D

L

F

L

A

K

P

P

G

G

D

D

R

A

V

V

V

I

F

P

S

L

F

F

V

V

P

P

A

Q

C

C

G

G

T

E

C

C

S

R

Y

S

C

C

A

L

E

S

G

T

R

K

P

G

V

N

L

L

C

C

V

I

P

K

G

N

A

D

A

L

A

S

N

S

S

S

A

P

G

T

T

G

L

L

L

M

S

A

G

D

A

G

R

T

W

R

R

F

D

T

S

C

D

K

G

G

A

K

E

A

C

I

H

H

H

F

L

I

G

G

V

T

S

S

A

T

F

F

A

T

E

E

R

Y

T

T

V

V

S

H

T

E

R

T

S

A

A

A

V

A

R

K

I

I

D

D

P

S

D

A

L

A

P

P

V

L

E

E

I

K

A

V

A

C

L

L

F

I

G

G

C

C

A

G

V

F

M

S

T

T

G

G

V

Y

G

G

A

A

A

V

V

L

N

Q

T

T

A

A

A

K

V

V

R

E

P

P

G

G

E

S

S

T

V

C

A

A

V

V

F

F

G

G

M

L

G

G

V

V

G

G

L

L

A

S

A

V

L

V

L

M

G

G

A

C

I

K

A

A

G

G

A

A

W

S

P

R

V

I

A

A

V

I

D

D

P

I

V

N

A

K

A

D

K

K

R

F

E

A

L

K

A

A

L

K

S

E

L

L

G

G

A

A

T

T

H

E

V

C

V

V

D

N

P

P

T

K

-

D

-

F

A

K

E

K

D

P

A

I

V

H

E

E

A

V

V

L

R

T

A

E

H

M

T

T

G

G

G

K

G

G

A

V

A

D

H

Y

A

S

V

F

E

E

T

V

V

I

G

G

S

R

E

I

K

E

V

T

L

M

E

T

Q

E

A

A

Y

L

F

A

A

S

T

C

-

H

V

Y

R

N

G

Y

G

G

Q

V

T

S

V

V

T

I

V

V

G

G

L

V

P

P

H

P

P

A

E

A

R

Q

M

K

L

I

S

S

I

F

P

D

A

P

V

M

S

-

L

L

V

I

A

F

E

S

E

G

R

R

T

T

L

W

R

K

G

G

S

S

Y

V

M

F

G

G

S

G

A

W

V

K

V

S

S

K

R

D

D

A

V

V

P

P

R

K

F

L

L

V

S

A

L

D

Y

Y

R

M

A

K

G

K

R

K

L

F

P

V

V

L

D

D

K

P

L

L

L

I

T

T

H

H

E

T

L

L

A

P

L

F

D

T

G

K

I

I

N

N

A

E

A

G

F

F

D

D

R

L

L

L

A

R

R

T

A

G

E

K

A

S

V

I

R

R

Q

T

V

V

I

L

Sites not aligning to the query:

1 modified: N-acetylserine

1cdoA Alcohol dehydrogenase (E.C.1.1.1.1) (ee isozyme) complexed with nicotinamide adenine dinucleotide (NAD), and zinc (see paper)
36% identity, 98% coverage: 5:375/379 of query aligns to 2:372/374 of 1cdoA

query
sites
1cdoA

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C

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C

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C

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x
W

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C

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T

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G

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A

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V

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D

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L

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G

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W

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V

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x
W

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A

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E

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A

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N

A

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I

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I

L

active site: C46 (= C50), H47 (= H51), T48 (≠ S52), Y51 (vs. gap), H68 (= H71), E69 (= E72), C98 (= C101), C101 (= C104), C104 (= C107), C112 (= C115), W116 (≠ A119), C175 (= C179), T179 (= T183), R369 (= R372)
binding nicotinamide-adenine-dinucleotide: C46 (= C50), H47 (= H51), T48 (≠ S52), C175 (= C179), T179 (= T183), G202 (= G206), V204 (= V208), D224 (= D228), L225 (≠ P229), V270 (= V272), G271 (= G273), V293 (= V294), W295 (≠ L296)
binding zinc ion: C46 (= C50), H68 (= H71), C98 (= C101), C104 (= C107), C112 (= C115)

P81600 Alcohol dehydrogenase class-3 chain H; Alcohol dehydrogenase class-III chain H; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Gadus morhua (Atlantic cod) (see 2 papers)
35% identity, 98% coverage: 5:377/379 of query aligns to 2:375/375 of P81600

query
sites
P81600

T

T

R

A

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G

A

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E

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M

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A

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-

G

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A

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F

Sites not aligning to the query:

1 modified: N-acetylalanine

P49645 Alcohol dehydrogenase 1; Alcohol dehydrogenase I; EC 1.1.1.1 from Apteryx australis (Southern brown kiwi) (see paper)
36% identity, 98% coverage: 5:377/379 of query aligns to 3:375/375 of P49645

query
sites
P49645

T

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C

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A

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A

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K

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A

A

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C

A

A

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L

A

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A

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G

A

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A

A

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G

A

A

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S

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R

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I

A

A

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I

D

D

P

I

V

N

A

K

A

D

K

K

R

F

E

A

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A

A

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K

S

E

L

L

G

G

A

A

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T

H

D

V

C

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V

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N

P

P

T

K

-

D

-

F

A

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K

D

P

A

I

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E

E

A

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A

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A

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C

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N

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A

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D

A

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M

S

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L

L

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T

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G

A

W

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S

S

K

R

D

D

A

V

V

P

P

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K

F

L

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S

A

L

D

Y

Y

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M

A

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K

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H

H

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P

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I

I

N

N

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F

F

Sites not aligning to the query:

2 modified: N-acetylserine

P80467 Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Saara hardwickii (Indian spiny-tailed lizard) (Uromastyx hardwickii) (see paper)
35% identity, 96% coverage: 15:377/379 of query aligns to 9:373/373 of P80467

query
sites
P80467

A

A

Q

V

P

A

Y

W

A

E

E

A

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P

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I

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I

A

A

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G

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H

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E

A

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A

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D

I

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E

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E

D

G

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K

F

F

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K

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P

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D

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I

F

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F

Y

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I

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P

A

Q

C

C

G

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E

C

C

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Y

F

C

C

A

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G

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N

L

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S

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S

D

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-

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Q

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Sites not aligning to the query:

1 modified: N-acetylalanine

Query Sequence

>WP_043767718.1 NCBI__GCF_000733765.1:WP_043767718.1
MTTITRGAVLREMGAAQPYAETRPLKIETLELAPPGPGEIQVRVRAAGICHSDLSVVNGS
RPRPMPMVLGHEAAGDVEAVGPDVADFAPGDRVVFSFVPACGTCSYCAEGRPVLCVPGAA
ANSAGTLLSGARRWRDSDGAECHHHLGVSAFAERTVVSTRSAVRIDPDLPVEIAALFGCA
VMTGVGAAVNTAAVRPGESVAVFGMGGVGLAALLGAIAAGAWPVVAVDPVAAKRELALSL
GATHVVDPTAEDAVEAVRAHTGGGAAHAVETVGSEKVLEQAYFATVRGGQTVTVGLPHPE
RMLSIPAVSLVAEERTLRGSYMGSAVVSRDVPRFLSLYRAGRLPVDKLLTHELALDGINA
AFDRLARAEAVRQVIRFDD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory