SitesBLAST

Comparing WP_043769276.1 NCBI__GCF_000733765.1:WP_043769276.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Found 1 hits to proteins with known functional sites (download)

O53289 Phosphoserine phosphatase SerB2; PSP; PSPase; O-phosphoserine phosphohydrolase; Protein-serine/threonine phosphatase; EC 3.1.3.3; EC 3.1.3.16 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
27% identity, 90% coverage: 3:202/222 of query aligns to 181:364/409 of O53289

query
sites
O53289

L

L

A

I

V

V

F

F

D
|
D

L
x
V

D
|
D

H
x
S

T

T

L

L

L

V

R

Q

G

G

D

E

S

-

D

-

Y

-

L

-

W

-

G

-

E

-

F

-

L

-

I

-

A

-

G

-

L

V

V

I

E

E

A

M

E

L

H

A

Y

A

R

R

A

A

Q

G

N

A

Q

Q

A

G

F

Q

M

V

A

A

Y

I

N

T

A

E

G

A

T

A

L

M

D

R

I

G

H

E

A

L

F

D

C

F

A

A

F

E

S

S

F

L

Q

Q

P

R

L

R

V

V

R

A

W

T

G

L

R

A

G

G

H

L

L

P

E

A

P

T

M

V

R

I

R

D

F

V

L

A

E

E

E

Q

E

L

I

-

A

-

P

-

R

E

V

L

A

M

P

P

G

G

A

A

R

R

P

T

L

T

L

I

D

R

R

T

H

L

R

R

Q

R

A

L

G

G

D

F

R

R

L

C

L

G

I

V

T

V

T
x
S

A

G

T

G

N

F

R

R

F

R

V

I

T

I

E

E

P

P

I

L

A

A

D

R

L

E

L

L

G

M

V

L

A

D

D

F

L

V

I

A

A

S

T

N

D

E

P

L

E

E

E

I

R

V

D

D

G

G

A

I

F

L

T

T

G

G

A

R

I

V

A

V

G

G

T

P

P

I

N

V

F

D

R

R

E

P

G

G

K
|
K

P

A

Q

K

R

A

L

L

R

R

Q

D

W

F

L

A

R

S

D

Q

N

Y

D

G

A

V

E

P

G

M

E

E

T

Q

L

T

V

V

C

A

Y

V

S

G

D
|
D

S

G

R

A

N

N

D
|
D

V

I

P

D

L

M

L

L

E

G

M

A

A

A

D

G

R

L

P

G

V

I

A

A

V

F

D

N

P

A

D

K

D

P

A

A

L

L

D185 (= D7) mutation to G: Completely abolishes enzymatic activity.; mutation to N: Completely abolishes enzymatic activity.

V186 (≠ L8) mutation to Q: Decreases enzymatic activity by 50%.

D187 (= D9) mutation to N: Decreases enzymatic activity by 15%.

S188 (≠ H10) mutation to A: No effect on enzymatic activity.

S273 (≠ T111) mutation to A: Completely abolishes enzymatic activity (PubMed:25521849). Decreases enzymatic activity by 60% (PubMed:25037224).

K318 (= K156) mutation to A: Decreases enzymatic activity by 50%.; mutation to E: Completely abolishes enzymatic activity.

D341 (= D179) mutation to G: Decreases enzymatic activity by 80%.; mutation to N: Decreases enzymatic activity by 85%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-345.

D345 (= D183) mutation to N: Decreases enzymatic activity by 55%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-341.

Sites not aligning to the query:

18 G→A: Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.

108 G→A: Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.

Query Sequence

>WP_043769276.1 NCBI__GCF_000733765.1:WP_043769276.1
MALAVFDLDHTLLRGDSDYLWGEFLIAAGLVEAEHYRAQNQAFMAAYNAGTLDIHAFCAF
SFQPLVRWGRGHLEPMRRRFLEEEIAPRVAPGARPLLDRHRQAGDRLLITTATNRFVTEP
IADLLGVADLIATDPEERDGAFTGAIAGTPNFREGKPQRLRQWLRDNDAEGETLVCYSDS
RNDVPLLEMADRPVAVDPDDALAAHARDRGWPIISLAEAGEN

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory

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