SitesBLAST
Comparing WP_043769901.1 NCBI__GCF_000733765.1:WP_043769901.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
7djsD Crystal structure of isopiperitenol dehydrogenase from pseudomonas aeruginosa complexed with NAD
45% identity, 99% coverage: 3:251/252 of query aligns to 3:250/251 of 7djsD
- binding nicotinamide-adenine-dinucleotide: G12 (= G12), G16 (= G16), I17 (≠ L17), D36 (= D36), L37 (≠ I37), C61 (≠ A61), D62 (= D62), V63 (= V63), N89 (= N89), A90 (= A90), T140 (≠ L138), S142 (= S140), Y155 (= Y153), K159 (= K157), A186 (≠ M184), V187 (= V185)
5itvA Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
38% identity, 100% coverage: 1:251/252 of query aligns to 3:254/255 of 5itvA
- active site: G18 (= G16), S141 (= S140), Y154 (= Y153), K158 (= K157)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G12), S17 (≠ A15), G18 (= G16), I19 (≠ L17), D38 (= D36), I39 (= I37), T61 (≠ A61), I63 (≠ V63), N89 (= N89), G91 (= G91), T139 (≠ L138), S141 (= S140), Y154 (= Y153), K158 (= K157), P184 (= P183), G185 (≠ M184), I186 (≠ V185), I187 (= I186)
5itvD Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
39% identity, 100% coverage: 1:251/252 of query aligns to 3:226/227 of 5itvD
- active site: G18 (= G16), S141 (= S140), Y154 (= Y153), K158 (= K157)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G12), S17 (≠ A15), G18 (= G16), I19 (≠ L17), D38 (= D36), I39 (= I37), T61 (≠ A61), D62 (= D62), I63 (≠ V63), N89 (= N89), T139 (≠ L138), S141 (= S140), Y154 (= Y153), K158 (= K157), P184 (= P183), G185 (≠ M184), I187 (= I186)
P40288 Glucose 1-dehydrogenase; EC 1.1.1.47 from Priestia megaterium (Bacillus megaterium) (see 2 papers)
39% identity, 98% coverage: 3:250/252 of query aligns to 5:251/261 of P40288
- 11:35 (vs. 9:33, 52% identical) binding NADP(+)
- E96 (= E93) mutation E->A,G,K: Heat stable.
- D108 (≠ E105) mutation to N: Heat stable.
- V112 (≠ L109) mutation to A: Heat stable.
- E133 (= E130) mutation to K: Heat stable.
- V183 (= V178) mutation to I: Heat stable.
- P194 (≠ D189) mutation to Q: Heat stable.
- E210 (= E201) mutation to K: Heat stable.
- Y217 (≠ R216) mutation to H: Heat stable.
Sites not aligning to the query:
- 252 Q→L: Heat stable.
- 253 Y→C: Heat stable.
- 258 A→G: Heat stable.
H9XP47 Meso-2,3-butanediol dehydrogenase; BDH; meso-2,3-BDH; (R,S)-butane-2,3-diol dehydrogenase; NAD(H)-dependent meso-2,3-BDH; SmBdh; EC 1.1.1.- from Serratia marcescens (see paper)
36% identity, 100% coverage: 1:251/252 of query aligns to 1:243/251 of H9XP47
- N15 (≠ A15) binding NAD(+)
- M17 (≠ L17) binding NAD(+)
- D36 (= D36) binding NAD(+)
- D60 (= D62) binding NAD(+)
- V61 (= V63) binding NAD(+)
- N87 (= N89) binding NAD(+)
- S138 (= S140) binding (R)-acetoin; binding (S)-acetoin
- V139 (≠ A141) mutation to Q: Retains 50% of activity with acetoin as substrate; when associated with A-247.
- S140 (≠ A142) binding (R)-acetoin
- Y151 (= Y153) binding (R)-acetoin; binding (S)-acetoin; binding NAD(+)
- K155 (= K157) binding NAD(+)
- V184 (≠ I186) binding NAD(+)
- T186 (= T188) binding NAD(+)
- RDK 197:199 (≠ KDT 195:197) mutation to SEAAGKPLGYGTET: Mimics longer alpha6 helix. Retains 3% of activity with acetoin as substrate.
Sites not aligning to the query:
- 247 Q→A: Retains 10% of activity with acetoin as substrate. Retains 50% of activity with acetoin as substrate; when associated with Q-139.
3ay6B Crystal structure of bacillus megaterium glucose dehydrogenase 4 a258f mutant in complex with nadh and d-glucose (see paper)
38% identity, 98% coverage: 3:250/252 of query aligns to 11:257/267 of 3ay6B
- active site: G24 (= G16), S151 (= S140), Y164 (= Y153), K168 (= K157)
- binding beta-D-glucopyranose: E102 (= E93), S151 (= S140), H153 (≠ A142), W158 (= W147), Y164 (= Y153), N202 (≠ G191), K205 (≠ R193)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G20 (= G12), T23 (≠ A15), G24 (= G16), L25 (= L17), Y45 (≠ I37), D71 (= D62), V72 (= V63), N98 (= N89), A99 (= A90), G100 (= G91), V101 (≠ I92), M149 (≠ L138), S151 (= S140), Y164 (= Y153), K168 (= K157), P194 (= P183), G195 (≠ M184), M197 (≠ I186), T199 (= T188), P200 (≠ D189), I201 (≠ M190), N202 (≠ G191)
1g6kA Crystal structure of glucose dehydrogenase mutant e96a complexed with NAD+
38% identity, 98% coverage: 3:250/252 of query aligns to 5:251/261 of 1g6kA
- active site: G18 (= G16), S145 (= S140), Y158 (= Y153), K162 (= K157)
- binding nicotinamide-adenine-dinucleotide: T17 (≠ A15), G18 (= G16), L19 (= L17), R39 (≠ D36), D65 (= D62), V66 (= V63), N92 (= N89), A93 (= A90), G94 (= G91), M143 (≠ L138), S145 (= S140), Y158 (= Y153), P188 (= P183), G189 (≠ M184), I191 (= I186), T193 (= T188)
6vspA Structure of serratia marcescens 2,3-butanediol dehydrogenase mutant q247a (see paper)
36% identity, 99% coverage: 2:251/252 of query aligns to 2:243/251 of 6vspA
- active site: G16 (= G16), S138 (= S140), Y151 (= Y153)
- binding nicotinamide-adenine-dinucleotide: G12 (= G12), N15 (≠ A15), G16 (= G16), M17 (≠ L17), D36 (= D36), W37 (≠ I37), W37 (≠ I37), A38 (≠ D38), I59 (≠ A61), D60 (= D62), V61 (= V63), N87 (= N89), A88 (= A90), G89 (= G91), V90 (≠ I92), V110 (≠ I112), T136 (≠ L138), S138 (= S140), Y151 (= Y153), K155 (= K157), P181 (= P183), S182 (≠ M184), L183 (≠ V185), V184 (≠ I186), T186 (= T188), N187 (≠ D189), M188 (= M190), T189 (≠ G191)
6xewA Structure of serratia marcescens 2,3-butanediol dehydrogenase (see paper)
36% identity, 99% coverage: 2:251/252 of query aligns to 2:243/251 of 6xewA
- active site: G16 (= G16), S138 (= S140), Y151 (= Y153)
- binding r,3-hydroxybutan-2-one: S138 (= S140), S140 (≠ A142), Y151 (= Y153)
- binding s,3-hydroxybutan-2-one: S138 (= S140), Y151 (= Y153), S182 (≠ M184)
- binding nicotinamide-adenine-dinucleotide: G12 (= G12), N15 (≠ A15), G16 (= G16), M17 (≠ L17), D36 (= D36), W37 (≠ I37), W37 (≠ I37), A38 (≠ D38), I59 (≠ A61), D60 (= D62), V61 (= V63), N87 (= N89), A88 (= A90), G89 (= G91), V110 (≠ I112), T136 (≠ L138), S138 (= S140), Y151 (= Y153), K155 (= K157), S182 (≠ M184), L183 (≠ V185), V184 (≠ I186), T186 (= T188), N187 (≠ D189), M188 (= M190), T189 (≠ G191)
6vspB Structure of serratia marcescens 2,3-butanediol dehydrogenase mutant q247a (see paper)
36% identity, 99% coverage: 2:251/252 of query aligns to 4:245/252 of 6vspB
8w0oA Gdh-105 crystal structure
38% identity, 98% coverage: 3:250/252 of query aligns to 5:251/259 of 8w0oA
- binding nicotinamide-adenine-dinucleotide: G14 (= G12), S17 (≠ A15), G18 (= G16), L19 (= L17), Y39 (≠ I37), Q43 (≠ S40), D65 (= D62), V66 (= V63), N92 (= N89), A93 (= A90), G94 (= G91), M143 (≠ L138), Y158 (= Y153), K162 (= K157), P188 (= P183), G189 (≠ M184), I191 (= I186), T193 (= T188)
A7IQH5 2-(S)-hydroxypropyl-CoM dehydrogenase 3; S-HPCDH 3; 2-[(S)-2-hydroxypropylthio]ethanesulfonate dehydrogenase 3; Aliphatic epoxide carboxylation component IV; Epoxide carboxylase component IV; SHPCDH3; EC 1.1.1.269 from Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2) (see 2 papers)
40% identity, 91% coverage: 22:251/252 of query aligns to 27:254/255 of A7IQH5
- D38 (= D36) binding NAD(+)
- DV 64:65 (= DV 62:63) binding NAD(+)
- N91 (= N89) binding NAD(+)
- S143 (= S140) binding (S)-2-hydroxypropyl-coenzyme M; mutation to A: Retains very weak activity.
- Y156 (= Y153) binding (S)-2-hydroxypropyl-coenzyme M; mutation to A: Retains some activity but with more than 2200-fold decrease in catalytic efficiency.; mutation to F: Loss of activity.
- K160 (= K157) binding NAD(+); mutation to A: Loss of activity.
- T188 (≠ M184) binding (S)-2-hydroxypropyl-coenzyme M
- VTSTG 189:193 (≠ VIATD 185:189) binding NAD(+)
- R211 (≠ D208) mutation to A: Severely impaired in the oxidation of S-HPC or reduction of 2-KPC but largely unaffected in the oxidation and reduction of aliphatic alcohols and ketones.
- K214 (≠ D211) mutation to A: Severely impaired in the oxidation of S-HPC or reduction of 2-KPC but largely unaffected in the oxidation and reduction of aliphatic alcohols and ketones.
- Y215 (≠ A212) binding (S)-2-hydroxypropyl-coenzyme M
Sites not aligning to the query:
4ituA Crystal structure of s-2-hydroxypropyl coenzyme m dehydrogenase (s- hpcdh) bound to s-hpc and nadh (see paper)
40% identity, 89% coverage: 28:251/252 of query aligns to 28:252/253 of 4ituA
- active site: N113 (= N113), S141 (= S140), Y154 (= Y153), K158 (= K157)
- binding 2-{[(2S)-2-hydroxypropyl]sulfanyl}ethanesulfonic acid: S141 (= S140), Y154 (= Y153), T186 (≠ M184), R209 (≠ D208), Y213 (≠ A212)
- binding 1,4-dihydronicotinamide adenine dinucleotide: D36 (= D36), L37 (≠ I37), D62 (= D62), V63 (= V63), N89 (= N89), V112 (≠ I112), F139 (≠ L138), S141 (= S140), Y154 (= Y153), K158 (= K157), P184 (= P183), T186 (≠ M184), V187 (= V185), T190 (= T188), M192 (= M190)
Sites not aligning to the query:
4gh5A Crystal structure of s-2-hydroxypropyl coenzyme m dehydrogenase (s- hpcdh) (see paper)
39% identity, 89% coverage: 28:251/252 of query aligns to 28:247/248 of 4gh5A
- active site: N113 (= N113), S141 (= S140), Y154 (= Y153), K158 (= K157)
- binding nicotinamide-adenine-dinucleotide: D36 (= D36), L37 (≠ I37), A61 (= A61), D62 (= D62), V63 (= V63), N89 (= N89), A90 (= A90), V112 (≠ I112), F139 (≠ L138), S141 (= S140), Y154 (= Y153), K158 (= K157), P184 (= P183), V187 (= V185), T190 (= T188), G191 (≠ D189), M192 (= M190)
Sites not aligning to the query:
8cxaA Crystal structure of 3-oxoacyl-[acyl-carrier-protein] reductase from mycobacterium smegmatis with bound NAD
37% identity, 98% coverage: 1:248/252 of query aligns to 1:247/251 of 8cxaA
- binding nicotinamide-adenine-dinucleotide: G12 (= G12), Q15 (≠ A15), G16 (= G16), I17 (≠ L17), D36 (= D36), V63 (= V63), N89 (= N89), A91 (≠ G91), S94 (≠ T94), I142 (≠ L138), S143 (≠ A139), S144 (= S140), Y157 (= Y153), K161 (= K157), P187 (= P183), H188 (≠ M184), I190 (= I186), I194 (≠ M190)
6ixmC Crystal structure of the ketone reductase chkred20 from the genome of chryseobacterium sp. Ca49 complexed with NAD (see paper)
36% identity, 99% coverage: 3:251/252 of query aligns to 3:247/248 of 6ixmC
- active site: G16 (= G16), S142 (= S140), Y155 (= Y153), K159 (= K157)
- binding nicotinamide-adenine-dinucleotide: G12 (= G12), S15 (≠ A15), G16 (= G16), I17 (≠ L17), D36 (= D36), I37 (= I37), A61 (= A61), D62 (= D62), T63 (≠ V63), N89 (= N89), A90 (= A90), M140 (≠ L138), S142 (= S140), Y155 (= Y153), K159 (= K157), P185 (= P183), A186 (≠ M184), Y187 (≠ V185), I188 (= I186), L192 (≠ M190)
5t5qC Crystal structure of short-chain dehydrogenase/reductase sdr:glucose/ribitol dehydrogenase from brucella melitensis
36% identity, 98% coverage: 1:248/252 of query aligns to 3:241/245 of 5t5qC
- active site: G18 (= G16), S140 (= S140), N150 (≠ L150), Y153 (= Y153), K157 (= K157)
- binding nicotinamide-adenine-dinucleotide: N16 (≠ A14), G17 (≠ A15), G18 (= G16), I19 (≠ L17), D38 (= D36), L39 (≠ I37), D63 (= D62), A64 (≠ V63), S90 (≠ N89), I113 (= I112), Y153 (= Y153), K157 (= K157), P182 (= P183), I185 (= I186), T187 (= T188), M189 (= M190)
Q9KJF1 (2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA dehydrogenase subunit BbsD; (S,R)-2-(alpha-hydroxybenzyl)succinyl-CoA dehydrogenase subunit BbsD; EC 1.1.1.429 from Thauera aromatica (see 2 papers)
36% identity, 98% coverage: 1:248/252 of query aligns to 1:242/248 of Q9KJF1
- M1 (= M1) modified: Initiator methionine, Removed
- S15 (≠ A15) binding NAD(+)
- D36 (= D36) binding NAD(+)
- D62 (= D62) binding NAD(+)
- I63 (≠ V63) binding NAD(+)
- N89 (= N89) binding NAD(+)
- Y153 (= Y153) binding NAD(+)
- K157 (= K157) binding NAD(+)
Q48436 Diacetyl reductase [(S)-acetoin forming]; Acetoin(diacetyl) reductase; AR; Meso-2,3-butanediol dehydrogenase; EC 1.1.1.304 from Klebsiella pneumoniae (see paper)
35% identity, 97% coverage: 5:248/252 of query aligns to 2:252/256 of Q48436
- 6:33 (vs. 9:36, 39% identical) binding NAD(+)
- D59 (= D62) binding NAD(+)
- K156 (= K157) binding NAD(+)
7pcsB Structure of the heterotetrameric sdr family member bbscd (see paper)
36% identity, 98% coverage: 3:248/252 of query aligns to 2:241/247 of 7pcsB
- binding nicotinamide-adenine-dinucleotide: G11 (= G12), M16 (≠ L17), D35 (= D36), I36 (= I37), I62 (≠ V63), N88 (= N89), G90 (= G91), I138 (≠ L138), S140 (= S140), Y152 (= Y153), K156 (= K157), I185 (= I186)
Query Sequence
>WP_043769901.1 NCBI__GCF_000733765.1:WP_043769901.1
MQLTNKRVLITGAAAGLGRDFALRFATEGAVITVSDIDESGAQAVAAEIKAAGGQAHALR
ADVTVEADVARLVADAVAAMGGLDCLINNAGIETIKPVTDISEAEWDRLMAINVKGVFFG
CKHAFPHLAETHGNIINLASAAGLIGWPLLSLYCASKGAVIQMSKALSQEFREAGVRVNA
LCPMVIATDMGSRFKDTYEKEYGVPAGDMLDARQGRLGRPEEVTAAAVFLASDGASFVNG
VALPIDNGGTAG
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory