SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_043879478.1 NCBI__GCF_000010525.1:WP_043879478.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6gwiB The crystal structure of halomonas elongata amino-transferase (see paper)
59% identity, 96% coverage: 7:451/465 of query aligns to 2:445/450 of 6gwiB

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6gwiB

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active site: F18 (= F23), Y149 (= Y154), D255 (= D260), K284 (= K289)
binding pyridoxal-5'-phosphate: S115 (≠ G120), G116 (= G121), S117 (= S122), Y149 (= Y154), H150 (= H155), G151 (= G156), E222 (= E227), D255 (= D260), V257 (= V262), I258 (= I263), K284 (= K289)

7q9xAAA Probable aminotransferase
56% identity, 98% coverage: 6:460/465 of query aligns to 1:454/455 of 7q9xAAA

query
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7q9xAAA

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binding (3E)-4-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}-2-oxobut-3-enoic acid: L55 (= L60), W56 (= W61), S115 (≠ G120), G116 (= G121), S117 (= S122), Y149 (= Y154), G151 (= G156), E222 (= E227), D255 (= D260), V257 (= V262), I258 (= I263), K284 (= K289), F316 (≠ Y322), T317 (= T323), R412 (= R418)
binding pyridoxal-5'-phosphate: F316 (≠ Y322), T317 (= T323)

4a6tC Crystal structure of the omega transaminase from chromobacterium violaceum in complex with plp (see paper)
56% identity, 98% coverage: 6:460/465 of query aligns to 1:454/455 of 4a6tC

query
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4a6tC

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D

I

I

G

G

P

P

Y

Y

L

M

Q

Q

E

K

R

R

W

W

L

R

-

E

P

T

L

F

A

S

N

R

H

F

P

E

L

H

V

V

G

D

E

D

A

V

R

R

M

G

V

V

G

G

L

M

I

V

G

Q

A

A

L

F

E

T

L

L

T

V

P

K

H

N

K

K

E

A

T

K

R

R

A

E

K

L

F

F

P

P

G

-

E

D

V

F

G

G

T

E

V

I

G

G

L

T

I

L

A

C

R

R

D

D

I

I

S

F

F

F

R

R

E

N

G

N

L

L

V

I

M

M

R

R

A

A

V

C

R

G

D

D

S

H

L

I

I
x
V

L

S

A

A

P

P

P

P

F

L

T

V

L

M

S

T

E

R

E

A

E

E

A

V

D

D

D

E

L

M

I

L

A

A

T

V

V

A

G

E

R

R

V

C

L

L

D

E

Q

E

T

F

L

E

A

Q

V

T

V

L

R

K

D

A

R

R

G

G

L

L

active site: F18 (= F23), Y149 (= Y154), E222 (= E227), D255 (= D260), I258 (= I263), K284 (= K289), V419 (≠ I425)
binding pyridoxal-5'-phosphate: G116 (= G121), S117 (= S122), Y149 (= Y154), H150 (= H155), G151 (= G156), E222 (= E227), D255 (= D260), V257 (= V262), I258 (= I263), K284 (= K289)

6s4gA Crystal structure of the omega transaminase from chromobacterium violaceum in complex with pmp (see paper)
56% identity, 98% coverage: 7:460/465 of query aligns to 1:453/453 of 6s4gA

query
sites
6s4gA

S

T

T

T

A

S

H

Q

W

W

R

R

H

E

L

L

D

D

A

A

A

A

H

H

H

H

L

L

H

H

P

P

F
|
F

S

T

D

D

T

T

K

A

G

S

L

L

N

N

A

Q

E

A

G

G

V

A

R

R

V

V

I

M

T

T

R

R

A

G

E

E

G

G

V

V

Y

Y

I

L

F

W

D

D

S

S

E

E

G

G

N

N

R

K

I

I

L

I

D

D

G

G

M

M

S

A

G

G

L

L

W

W

C

C

S

V

A

N

V

V

G

G

H

Y

G

G

R

R

H

K

A

D

I

F

V

A

D

E

A

A

I

A

A

R

D

R

Q

Q

L

M

R

E

Q

E

L

L

D

P

Y

F

Y

Y

N

N

T

T

F

F

F

F

K

K

T

T

T

T

H

H

P

P

G

A

A

V

A

V

E

E

L

L

A

S

A

S

A

L

I

L

A

A

Q

E

V

V

A

T

P

P

E

A

G

G

F

F

E

D

R

R

V

V

F

F

F

Y

T

T

S

N

G
x
S

G
|
G

S
|
S

E

E

A

S

V

V

D

D

T

T

V

M

I

I

R

R

M

M

V

V

R

R

H

R

Y

Y

W

W

A

D

A

V

V

Q

G

G

K

K

P

P

G

E

K

K

H

K

I

T

F

L

I

I

A

G

R

R

K

W

N

N

A

G

Y
|
Y

H
|
H

G
|
G

S

S

T

T

I

I

G

G

G

G

A

A

S

S

L

L

G

G

G

G

M

M

A

K

P

Y

M

M

H

H

A

E

Q

Q

G

G

G

D

L

L

P

P

I

I

P

P

G

G

I

M

V

A

H

H

V

I

Q

E

Q

Q

P

P

Y

W

W

W

W

Y

G

K

E

H

G

G

G

K

N

D

M

M

S

T

P

P

E

D

E

E

F

F

G

G

L

V

F

V

A

A

A

A

Q

R

E

W

V

L

A

E

R

E

A

K

I

I

D

L

E

E

A

I

G

G

P

A

E

D

N

K

V

V

A

A

A

A

F

F

I

V

G

G

E
|
E

P

P

I

I

Q

Q

G

G

A

A

G

G

G

G

V

V

I

I

I

V

P

P

P

P

S

A

T

T

Y

Y

W

W

P

P

E

E

V

I

Q

E

K

R

I

I

C

C

R

R

E

K

R

Y

D

D

V

V

L

L

L

L

V

V

S

A

D
|
D

E

E

V
|
V

I
|
I

C

C

G

G

F

F

G

G

R

R

T

T

G

G

E

E

W

W

F

F

G

G

C

H

Q

Q

H

H

M

F

G

G

V

F

Q

Q

P

P

D

D

L

L

I

F

T

T

F

A

A

A

K
|
K

G

G

V

L

T

S

S

S

G

G

Y

Y

F

L

P

P

L

I

G

G

G

A

V

V

I

F

V

V

G

G

E

K

R

R

V

V

A

A

E

E

G

G

L

L

I

I

E

-

Q

A

G

G

G

G

E

D

F

F

H

N

H

H

G

G

Y
x
F

T
|
T

Y

Y

S

S

G

G

H

H

P

P

G

V

G

C

C

A

A

A

A

V

A

A

L

H

A

A

T

N

L

V

K

A

I

A

M

L

H

R

E

D

E

E

D

G

L

I

V

V

A

Q

R

R

V

V

K

K

C

D

E

D

I

I

G

G

P

P

Y

Y

L

M

Q

Q

E

K

R

R

W

W

L

R

-

E

P

T

L

F

A

S

N

R

H

F

P

E

L

H

V

V

G

D

E

D

A

V

R

R

M

G

V

V

G

G

L

M

I

V

G

Q

A

A

L

F

E

T

L

L

T

V

P

K

H

N

K

K

E

A

T

K

R

R

A

E

K

L

F

F

P

P

G

-

E

D

V

F

G

G

T

E

V

I

G

G

L

T

I

L

A

C

R

R

D

D

I

I

S

F

F

F

R

R

E

N

G

N

L

L

V

I

M

M

R

R

A

A

V

C

R

G

D

D

S

H

L

I

I

V

L

S

A

A

P

P

P

P

F

L

T

V

L

M

S

T

E

R

E

A

E

E

A

V

D

D

D

E

L

M

I

L

A

A

T

V

V

A

G

E

R

R

V

C

L

L

D

E

Q

E

T

F

L

E

A

Q

V

T

V

L

R

K

D

A

R

R

G

G

L

L

active site: F17 (= F23), Y148 (= Y154), D254 (= D260), K283 (= K289)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S114 (≠ G120), G115 (= G121), S116 (= S122), Y148 (= Y154), H149 (= H155), G150 (= G156), E221 (= E227), D254 (= D260), V256 (= V262), I257 (= I263), K283 (= K289), F315 (≠ Y322), T316 (= T323)

7qx0B Transaminase structure of plurienzyme (tr2e2) in complex with plp (see paper)
55% identity, 96% coverage: 7:451/465 of query aligns to 1:438/443 of 7qx0B

query
sites
7qx0B

S

S

T

T

A

A

H

D

W

W

R

Q

H

R

L

L

D

D

A

A

A

A

H

H

H

H

L

L

H

H

P

P

F

F

S

T

D

D

T

Y

K

G

G

E

L

L

N

N

A

T

E

K

G

G

V

S

R

R

V

I

I

I

T

T

R

R

A

A

E

E

G

G

V

C

Y

Y

I

L

F

W

D

D

S

S

E

D

G

G

N

N

R

Q

I

I

L

L

D

D

G

G

M

M

S

A

G

G

L

L

W

W

C

C

S

V

A

N

V

I

G

G

H

Y

G

G

R

R

H

K

A

E

I

L

V

A

D

E

A

V

I

A

A

Y

D

R

Q

Q

L

M

R

Q

Q

E

L

L

D

P

Y

Y

Y

Y

N

N

T

N

F

F

F

F

K

Q

T

C

T

S

H

H

P

P

G

P

A

A

A

I

E

E

L

L

A

S

A

R

A

L

I

L

A

S

Q

E

V

V

A

T

P

P

E

K

G

H

F

M

E

N

R

H

V

V

F

F

F

F

T

T

S

G

G
x
S

G
|
G

S
|
S

E

D

A

S

V

N

D

D

T

T

V

I

I

L

R

R

M

M

V

V

R

R

H

Y

Y

Y

W

W

A

K

A

L

V

L

G

G

K

K

P

P

G

Y

K

K

H

K

I

V

F

V

I

I

A

S

R

R

K

E

N

N

A

A

Y
|
Y

H
|
H

G

G

S

S

T

T

I

V

G

A

G

G

A

A

S

S

L

L

G

S

G

G

M

M

A

K

P

A

M

M

H

H

A

S

Q

H

G

G

G

D

L

L

P

P

I

I

P

P

G

G

I

I

V

E

H

H

V

I

Q

E

Q

Q

P

P

Y

Y

W

H

W

F

G

G

E

R

G

A

G

P

N

D

M

M

S

D

P

P

E

A

E

E

F

F

G

G

L

R

F

Q

A

A

A

A

Q

Q

E

A

V

L

A

E

R

R

A

K

I

I

D

D

E

E

A

I

G

G

P

E

E

C

N

N

V

V

A

A

A

A

F

F

I

I

G

A

E
|
E

P

P

I

I

Q

Q

G

G

A

A

G

G

G

G

V

V

I

I

I

I

P

P

P

P

S

D

T

S

Y

Y

W

W

P

P

E

E

V

I

Q

K

K

R

I

I

C

C

R

A

E

E

R

R

D

D

V

I

L

L

L

L

V

I

S

V

D
|
D

E

E

V
|
V

I
|
I

C

T

G

G

F

F

G

G

R

R

T

L

G

G

E

T

W

W

F

F

G

G

C

S

Q

Q

H

Y

M

Y

G

D

V

L

Q

Q

P

P

D

D

L

L

I

M

T

P

F

I

A

A

K
|
K

G

G

V

L

T

S

S

S

G

G

Y

Y

F

M

P

P

L

I

G

G

G

G

V

V

I

M

V

V

G

S

E

D

R

R

V

V

A

A

E

K

G

V

L

V

I

I

E

E

Q

E

G

G

G

G

E

E

F

-

H

-

H

-

G

-

Y

-

T

-

Y

Y

S

S

G

G

H

H

P

P

G

V

G

A

C

A

A

A

A

V

A

A

L

A

A

E

T

N

L

I

K

R

I

I

M

M

H

R

E

D

E

E

D

G

L

I

V

I

A

E

R

R

V

A

K

G

C

A

E

E

I

I

G

A

P

P

Y

Y

L

L

Q

Q

E

A

R

R

W

W

L

R

P

E

L

L

A

G

N

E

H

H

P

P

L

L

V

V

G

G

E

E

A

A

R

R

M

G

V

V

G

G

L

M

I

V

G

A

A

A

L

L

E

E

L

L

T

V

P

K

H

S

K

K

E

Q

T

P

R

L

A

E

K

R

F

F

P

E

G

-

E

E

V

P

G

G

T

K

V

V

G

G

L

S

I

L

A

C

R

R

D

D

I

L

S

S

F

V

R

K

E

N

G

G

L

L

V

V

M

M

R

R

A

A

V

V

R

G

D

G

S

T

L

M

I

I

L

I

A

S

P

P

P

P

F

L

T

V

L

L

S

S

E

R

E

E

E

Q

A

V

D

D

D

E

L

L

I

I

A

D

T

K

V

A

G

R

R

R

V

T

L

L

D

D

Q

E

T

T

binding pyridoxal-5'-phosphate: S114 (≠ G120), G115 (= G121), S116 (= S122), Y148 (= Y154), H149 (= H155), E221 (= E227), D254 (= D260), V256 (= V262), I257 (= I263), K283 (= K289)

7qx3A Structure of the transaminase tr2e2 with eos (see paper)
56% identity, 90% coverage: 35:451/465 of query aligns to 2:417/422 of 7qx3A

query
sites
7qx3A

R

R

V

I

I

I

T

T

R

R

A

A

E

E

G

G

V

C

Y

Y

I

L

F

W

D

D

S

S

E

D

G

G

N

N

R

Q

I

I

L

L

D

D

G

G

M

M

S

A

G

G

L

L

W

W

C

C

S

V

A

N

V

I

G

G

H

Y

G

G

R

R

H

K

A

E

I

L

V

A

D

E

A

V

I

A

A

Y

D

R

Q

Q

L

M

R

Q

Q

E

L

L

D

P

Y

Y

Y

Y

N

N

T

N

F

F

F

F

K

Q

T

C

T

S

H

H

P

P

G

P

A

A

A

I

E

E

L

L

A

S

A

R

A

L

I

L

A

S

Q

E

V

V

A

T

P

P

E

K

G

H

F

M

E

N

R

H

V

V

F

F

F

F

T

T

S

G

G

S

G

G

S

S

E

D

A

S

V

N

D

D

T

T

V

I

I

L

R

R

M

M

V

V

R

R

H

Y

Y

Y

W

W

A

K

A

L

V

L

G

G

K

K

P

P

G

Y

K

K

H

K

I

V

F

V

I

I

A

S

R

R

K

E

N

N

A

A

Y
|
Y

H

H

G

G

S

S

T

T

I

V

G

A

G

G

A

A

S

S

L

L

G

S

G

G

M

M

A

K

P

A

M

M

H

H

A

S

Q

H

G

G

G

D

L

L

P

P

I

I

P

P

G

G

I

I

V

E

H

H

V

I

Q

E

Q

Q

P

P

Y

Y

W

H

W

F

G

G

E

R

G

A

G

P

N

D

M

M

S

D

P

P

E

A

E

E

F

F

G

G

L

R

F

Q

A

A

A

A

Q

Q

E

A

V

L

A

E

R

R

A

K

I

I

D

D

E

E

A

I

G

G

P

E

E

C

N

N

V

V

A

A

A

A

F

F

I

I

G

A

E

E

P

P

I

I

Q

Q

G

G

A
|
A

G

G

G

G

V

V

I

I

I

I

P

P

P

P

S

D

T

S

Y

Y

W

W

P

P

E

E

V

I

Q

K

K

R

I

I

C

C

R

A

E

E

R

R

D

D

V

I

L

L

L

L

V

I

S

V

D

D

E

E

V

V

I

I

C

T

G

G

F

F

G

G

R

R

T

L

G

G

E

T

W

W

F

F

G

G

C

S

Q

Q

H

Y

M

Y

G

D

V

L

Q

Q

P

P

D

D

L

L

I

M

T

P

F

I

A

A

K
|
K

G

G

V

L

T

S

S

S

G

G

Y

Y

F

M

P

P

L

I

G

G

G

G

V

V

I

M

V

V

G

S

E

D

R

R

V

V

A

A

E

K

G

V

L

V

I

I

E

E

Q

E

G

G

G

G

E

E

F

F

H

F

H

H

G

G

Y

Y

T

T

Y

Y

S

S

G

G

H

H

P

P

G

V

G

A

C

A

A

A

A

V

A

A

L

A

A

E

T

N

L

I

K

R

I

I

M

M

H

R

E

D

E

E

D

G

L

I

V

I

A

E

R

R

V

A

K

G

C

A

E

E

I

I

G

A

P

P

Y

Y

L

L

Q

Q

E

A

R

R

W

W

L

R

P

E

L

L

A

G

N

E

H

H

P

P

L

L

V

V

G

G

E

E

A

A

R

R

M

G

V

V

G

G

L

M

I

V

G

A

A

A

L

L

E

E

L

L

T

V

P

K

H

S

K

K

E

Q

T

P

R

L

A

E

K

R

F

F

P

E

G

-

E

E

V

P

G

G

T

K

V

V

G

G

L

S

I

L

A

C

R

R

D

D

I

L

S

S

F

V

R

K

E

N

G

G

L

L

V

V

M

M

R

R

A

A

V

V

R

G

D

G

S

T

L

M

I

I

L

I

A

S

P

P

P

P

F

L

T

V

L

L

S

S

E

R

E

E

E

Q

A

V

D

D

D

E

L

L

I

I

A

D

T

K

V

A

G

R

R

R

V

T

L

L

D

D

Q

E

T

T

binding 2-azanylethyl hydrogen sulfate: Y121 (= Y154), A199 (= A232), K256 (= K289)

4ba5A Crystal structure of omega-transaminase from chromobacterium violaceum (see paper)
56% identity, 92% coverage: 35:460/465 of query aligns to 2:426/427 of 4ba5A

query
sites
4ba5A

R

R

V

V

I

M

T

T

R

R

A

G

E

E

G

G

V

V

Y

Y

I

L

F

W

D

D

S

S

E

E

G

G

N

N

R

K

I

I

L

I

D

D

G

G

M

M

S

A

G

G

L
|
L

W
|
W

C

C

S

V

A

N

V

V

G

G

H

Y

G

G

R

R

H

K

A

D

I

F

V

A

D

E

A

A

I

A

A

R

D

R

Q

Q

L

M

R

E

Q

E

L

L

D

P

Y

F

Y

Y

N

N

T

T

F

F

F

F

K

K

T

T

T

T

H

H

P

P

G

A

A

V

A

V

E

E

L

L

A

S

A

S

A

L

I

L

A

A

Q

E

V

V

A

T

P

P

E

A

G

G

F

F

E

D

R

R

V

V

F

F

F

Y

T

T

S

N

G

S

G
|
G

S
|
S

E

E

A

S

V

V

D

D

T

T

V

M

I

I

R

R

M

M

V

V

R

R

H

R

Y

Y

W

W

A

D

A

V

V

Q

G

G

K

K

P

P

G

E

K

K

H

K

I

T

F

L

I

I

A

G

R

R

K

W

N

N

A

G

Y
|
Y

H
|
H

G
|
G

S

S

T

T

I

I

G

G

G

G

A

A

S

S

L

L

G

G

G

G

M

M

A

K

P

Y

M

M

H

H

A

E

Q

Q

G

G

G

D

L

L

P

P

I

I

P

P

G

G

I

M

V

A

H

H

V

I

Q

E

Q

Q

P

P

Y

W

W

W

W

Y

G

K

E

H

G

G

G

K

N

D

M

M

S

T

P

P

E

D

E

E

F

F

G

G

L

V

F

V

A

A

A

A

Q

R

E

W

V

L

A

E

R

E

A

K

I

I

D

L

E

E

A

I

G

G

P

A

E

D

N

K

V

V

A

A

A

A

F

F

I

V

G

G

E
|
E

P

P

I

I

Q

Q

G

G

A
|
A

G

G

G

G

V

V

I

I

I

V

P

P

P

P

S

A

T

T

Y

Y

W

W

P

P

E

E

V

I

Q

E

K

R

I

I

C

C

R

R

E

K

R

Y

D

D

V

V

L

L

L

L

V

V

S

A

D
|
D

E

E

V
|
V

I
|
I

C

C

G

G

F

F

G

G

R

R

T

T

G

G

E

E

W

W

F

F

G

G

C

H

Q

Q

H

H

M

F

G

G

V

F

Q

Q

P

P

D

D

L

L

I

F

T

T

F

A

A

A

K
|
K

G

G

V

L

T

S

S

S

G

G

Y

Y

F

L

P

P

L

I

G

G

G

A

V

V

I

F

V

V

G

G

E

K

R

R

V

V

A

A

E

E

G

G

L

L

I

I

E

-

Q

A

G

G

G

G

E

D

F

F

H

N

H

H

G

G

Y

F

T

T

Y

Y

S

S

G

G

H

H

P

P

G

V

G

C

C

A

A

A

A

V

A

A

L

H

A

A

T

N

L

V

K

A

I

A

M

L

H

R

E

D

E

E

D

G

L

I

V

V

A

Q

R

R

V

V

K

K

C

D

E

D

I

I

G

G

P

P

Y

Y

L

M

Q

Q

E

K

R

R

W

W

L

R

-

E

P

T

L

F

A

S

N

R

H

F

P

E

L

H

V

V

G

D

E

D

A

V

R

R

M

G

V

V

G

G

L

M

I

V

G

Q

A

A

L

F

E

T

L

L

T

V

P

K

H

N

K

K

E

A

T

K

R

R

A

E

K

L

F

F

P

P

G

-

E

D

V

F

G

G

T

E

V

I

G

G

L

T

I

L

A

C

R

R

D

D

I

I

S

F

F

F

R

R

E

N

G

N

L

L

V

I

M

M

R
|
R

A

A

V

C

R

G

D

D

S

H

L

I

I
x
V

L

S

A

A

P

P

P

P

F

L

T

V

L

M

S

T

E

R

E

A

E

E

A

V

D

D

D

E

L

M

I

L

A

A

T

V

V

A

G

E

R

R

V

C

L

L

D

E

Q

E

T

F

L

E

A

Q

V

T

V

L

R

K

D

A

R

R

G

G

L

L

active site: Y121 (= Y154), E194 (= E227), D227 (= D260), I230 (= I263), K256 (= K289), V391 (≠ I425)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: L27 (= L60), W28 (= W61), G88 (= G121), S89 (= S122), Y121 (= Y154), H122 (= H155), G123 (= G156), E194 (= E227), A199 (= A232), D227 (= D260), V229 (= V262), R384 (= R418)

4a6rA Crystal structure of the omega transaminase from chromobacterium violaceum in the apo form, crystallised from polyacrylic acid (see paper)
55% identity, 92% coverage: 35:460/465 of query aligns to 1:422/423 of 4a6rA

query
sites
4a6rA

R

R

V

V

I

M

T

T

R

R

A

G

E

E

G

G

V

V

Y

Y

I

L

F

W

D

D

S

S

E

E

G

G

N

N

R

K

I

I

L

I

D

D

G

G

M

M

S

A

G

G

L

L

W

W

C

C

S

V

A

N

V

V

G

G

H

Y

G

G

R

R

H

K

A

D

I

F

V

A

D

E

A

A

I

A

A

R

D

R

Q

Q

L

M

R

E

Q

E

L

L

D

P

Y

F

Y

Y

N

N

T

T

F

F

F

F

K

K

T

T

T

T

H

H

P

P

G

A

A

V

A

V

E

E

L

L

A

S

A

S

A

L

I

L

A

A

Q

E

V

V

A

T

P

P

E

A

G

G

F

F

E

D

R

R

V

V

F

F

F

Y

T
|
T

S
x
N

G
x
S

G

G

S
|
S

E
|
E

A

S

V

V

D

D

T
|
T

V

M

I

I

R
|
R

M

M

V

V

R

R

H

R

Y

Y

W

W

A

D

A

V

V

Q

G

G

K

K

P

P

G

E

K

K

H

K

I

T

F

L

I

I

A

G

R

R

K

W

N

N

A

G

Y
|
Y

H

H

G

G

S
|
S

T
|
T

I

I

G

G

G

G

A

A

S

S

L

L

G

G

G

G

M

M

A

K

P

Y

M

M

H

H

A

-

Q

-

G

-

G

D

L

L

P

P

I

I

P

P

G

G

I

M

V

A

H

H

V

I

Q

E

Q

Q

P

P

Y

W

W

W

W

Y

G

K

E

H

G

G

G

K

N

D

M

M

S

T

P

P

E

D

E

E

F

F

G

G

L

V

F

V

A

A

A

A

Q

R

E

W

V

L

A

E

R

E

A

K

I

I

D

L

E

E

A

I

G

G

P

A

E

D

N

K

V

V

A

A

A

A

F

F

I

V

G

G

E
|
E

P

P

I

I

Q

Q

G

G

A

A

G

G

G

G

V

V

I

I

I

V

P

P

P

P

S

A

T

T

Y

Y

W

W

P

P

E

E

V

I

Q

E

K

R

I

I

C

C

R

R

E

K

R

Y

D

D

V

V

L

L

L

L

V

V

S

A

D
|
D

E

E

V

V

I
|
I

C

C

G

G

F

F

G

G

R

R

T

T

G

G

E

E

W

W

F

F

G

G

C

H

Q

Q

H

H

M

F

G

G

V

F

Q

Q

P

P

D

D

L

L

I

F

T

T

F

A

A

A

K
|
K

G

G

V

L

T

S

S

S

G

G

Y

Y

F

L

P

P

L

I

G

G

G

A

V

V

I

F

V

V

G

G

E

K

R

R

V

V

A

A

E

E

G

G

L

L

I

I

E

-

Q

A

G

G

G

G

E

D

F

F

H

N

H

H

G

G

Y

F

T

T

Y

Y

S

S

G

G

H

H

P

P

G

V

G

C

C

A

A

A

A

V

A

A

L

H

A

A

T

N

L

V

K

A

I

A

M

L

H

R

E

D

E

E

D

G

L

I

V

V

A

Q

R

R

V

V

K

K

C

D

E

D

I

I

G

G

P

P

Y

Y

L

M

Q

Q

E

K

R

R

W

W

L

R

-

E

P

T

L

F

A

S

N

R

H

F

P

E

L

H

V

V

G

D

E

D

A

V

R

R

M

G

V

V

G

G

L

M

I

V

G

Q

A

A

L

F

E

T

L

L

T

V

P

K

H

N

K

K

E

A

T

K

R

R

A

E

K

L

F

F

P

P

G

-

E

D

V

F

G

G

T

E

V

I

G

G

L

T

I

L

A

C

R

R

D

D

I

I

S

F

F

F

R

R

E

N

G

N

L

L

V

I

M

M

R

R

A

A

V

C

R

G

D

D

S

H

L

I

I
x
V

L

S

A

A

P

P

P

P

F

L

T

V

L

M

S

T

E

R

E

A

E

E

A

V

D

D

D

E

L

M

I

L

A

A

T

V

V

A

G

E

R

R

V

C

L

L

D

E

Q

E

T

F

L

E

A

Q

V

T

V

L

R

K

D

A

R

R

G

G

L

L

active site: Y120 (= Y154), E190 (= E227), D223 (= D260), I226 (= I263), K252 (= K289), V387 (≠ I425)
binding polyacrylic acid: T84 (= T118), N85 (≠ S119), S86 (≠ G120), S88 (= S122), E89 (= E123), T93 (= T127), R96 (= R130), Y120 (= Y154), S123 (= S157), T124 (= T158)

7ypmA Crystal structure of transaminase cc1012 complexed with plp and l- alanine (see paper)
52% identity, 97% coverage: 6:456/465 of query aligns to 1:452/454 of 7ypmA

query
sites
7ypmA

N

H

S

D

T

I

A

A

H

E

W

L

R

K

H

R

L

L

D

D

A

L

A

A

H

H

H

H

L

L

H

P

P

A

F

Q

S

A

D

D

T

H

K

K

G

V

L

I

N

A

A

E

E

Q

G

G

-

G

V

S

R

R

V

I

I

I

T

T

R

R

A

A

E

E

G

G

V

V

Y

Y

I

I

F

H

D

D

S

G

E

E

G

G

N

H

R

Q

I

I

L

L

D

D

G

G

M

M

S

A

G

G

L

L

W
|
W

C

C

S

V

A

N

V

V

G

G

H

Y

G

G

R

R

H

E

A

E

I

L

V

A

D

K

A

A

I

A

A

Y

D

D

Q

Q

L

M

R

L

Q

E

L

L

D

P

Y

Y

Y

Y

N

N

T

T

F

F

F

F

K

K

T

T

T

A

H

T

P

P

G

P

A

P

A

I

E

E

L

L

A

A

A

A

A

K

I

I

A

A

Q

Q

V

K

A

M

P

G

E

G

G

H

F

L

E

S

R

H

V

V

F

F

F

Y

T

N

S

S

G
x
S

G
|
G

S
|
S

E

E

A

A

V

N

D

D

T

T

V

V

I

F

R

R

M

L

V

V

R

R

H

H

Y

F

W

W

A

K

A

L

V

K

G

G

K

E

P

P

G

S

K

R

H

T

I

V

F

F

I

I

A

S

R

R

K

W

N

N

A

A

Y
|
Y

H

H

G
|
G

S

S

T

T

I

V

G

A

G

G

A

V

S

S

L

L

G

G

G

G

M

M

A

K

P

H

M

M

H

H

A

K

Q

Q

G

G

G

D

L

L

P

P

I

I

P

A

G

G

I

V

V

E

H

H

V

V

Q

M

Q

Q

P

P

Y

Y

W

Q

W

F

G

G

E

D

G

G

G

F

N

G

M

E

S

D

P

P

E

A

E

A

F

F

G

R

L

D

F

R

A

A

A

V

Q

Q

E

A

V

I

A

E

R

D

A

K

I

I

D

L

E

E

A

V

G

G

P

P

E

E

N

N

V

V

A

A

A

A

F

F

I

I

G

G

E
|
E

P

P

I

V

Q

Q

G

G

A

A

G

G

G

G

V

V

I

I

I

I

P

P

P

P

S

D

T

G

Y

Y

W

W

P

P

E

A

V

V

Q

E

K

A

I

L

C

C

R

R

E

K

R

Y

D

G

V

I

L

L

L

L

V

V

S

C

D
|
D

E

E

V
|
V

I
|
I

C

C

G

G

F

F

G

G

R

R

T

L

G

G

E

Q

W

W

F

F

G

G

C

H

Q

Q

H

H

M

Y

G

G

V

I

Q

K

P

P

D

D

L

L

I

I

T

A

F

M

A

A

K
|
K

G

G

V

L

T

S

S

S

G

G

Y

Y

F

L

P

P

L

I

G

S

G

A

V

V

I

G

V

V

G

A

E

D

R

H

V

I

A

V

E

A

G

E

L

L

I

R

E

E

Q

K

G

G

G

G

E

D

F

F

H

I

H

H

G

G

Y

F

T

T

Y

Y

S

S

G

G

H

H

P

P

G

T

G

A

C

A

A

A

A

V

A

A

L

L

A

K

T

N

L

I

K

E

I

I

M

M

H

E

E

R

E

E

D

G

L

L

V

V

A

E

R

R

V

T

K

R

C

D

E

E

I

T

G

G

P

P

Y

Y

L

L

Q

A

E

Q

R

A

W

L

L

A

P

S

L

L

A

N

N

D

H

H

P

P

L

L

V

V

G

G

E

E

A

V

R

R

M

S

V

L

G

G

L

L

I

I

G

G

A

A

L

V

E

E

L

I

T

V

P

R

H

E

K

K

E

G

T

T

R

N

A

H

K

R

F

F

P

L

G

D

E

K

V

E

G

G

T

E

V

A

G

G

L

P

I

I

A

V

R

R

D

D

I

L

S

C

F

I

R

K

E

N

G

G

L

L

V

M

M

V

R

R

A

A

V

I

R

R

D

D

S

S

L

I

I

V

L

C

A

C

P

P

P

P

F

L

T

I

L

I

S

T

E

K

E

A

E

Q

A

I

D

D

D

E

L

L

I

V

A

G

T

I

V

I

G

R

R

K

V

S

L

L

D

D

Q

E

T

A

L

E

A

P

V

V

V

L

R

R

binding alanine: W57 (= W61), Y150 (= Y154)
binding pyridoxal-5'-phosphate: S116 (≠ G120), G117 (= G121), S118 (= S122), Y150 (= Y154), G152 (= G156), E223 (= E227), D256 (= D260), V258 (= V262), I259 (= I263), K285 (= K289)

7ypnD Crystal structure of transaminase cc1012 mutant m9 complexed with plp (see paper)
51% identity, 97% coverage: 6:456/465 of query aligns to 1:452/455 of 7ypnD

query
sites
7ypnD

N

H

S

D

T

I

A

A

H

E

W

L

R

K

H

R

L

L

D

D

A

L

A

A

H

H

H

H

L

L

H

P

P

A

F

F

S

A

D

D

T

H

K

K

G

V

L

I

N

A

A

E

E

Q

G

G

-

G

V

S

R

R

V

I

I

I

T

T

R

R

A

A

E

E

G

G

V

V

Y

Y

I

I

F

H

D

D

S

G

E

E

G

G

N

H

R

Q

I

I

L

L

D

D

G

G

M

W

S

A

G

G

L

L

W

F

C

C

S

V

A

N

V

V

G

G

H

Y

G

G

R

R

H

E

A

E

I

L

V

A

D

K

A

A

I

A

A

Y

D

D

Q

Q

L

M

R

L

Q

E

L

L

D

P

Y

Y

Y

Y

N

N

T

T

F

F

F

F

K

K

T

T

T

A

H

T

P

P

G

P

A

P

A

I

E

E

L

L

A

A

A

A

A

K

I

I

A

A

Q

Q

V

K

A

M

P

G

E

G

G

H

F

L

E

S

R

H

V

V

F

F

F

Y

T

N

S

S

G
x
S

G
|
G

S
|
S

E

E

A

A

V

N

D

D

T

T

V

V

I

F

R

R

M

L

V

V

R

R

H

H

Y

F

W

W

A

K

A

L

V

K

G

G

K

E

P

P

G

S

K

R

H

T

I

V

F

F

I

I

A

S

R

R

K

W

N

N

A

A

Y
|
Y

H
|
H

G
|
G

S

S

T

T

I

V

G

A

G

G

A

V

S

S

L

L

G

G

G

G

M

M

A

K

P

H

M

M

H

H

A

K

Q

Q

G

G

G

D

L

L

P

P

I

I

P

A

G

G

I

V

V

E

H

H

V

V

Q

M

Q

Q

P

P

Y

Y

W

Q

W

F

G

G

E

D

G

G

G

F

N

G

M

E

S

D

P

P

E

A

E

A

F

F

G

R

L

D

F

R

A

A

A

V

Q

Q

E

A

V

I

A

E

R

D

A

K

I

I

D

L

E

E

A

V

G

G

P

P

E

E

N

N

V

V

A

A

A

A

F

F

I

I

G

G

E
|
E

P

P

I

V

Q

Q

G

G

A

A

G

G

G

G

V

V

I

I

I

I

P

P

P

P

S

D

T

G

Y

Y

W

W

P

P

E

A

V

V

Q

E

K

A

I

L

C

C

R

R

E

K

R

Y

D

G

V

I

L

L

L

L

V

V

S

C

D
|
D

E

E

V
|
V

I

A

C

C

G

G

F

F

G

G

R

R

T

L

G

G

E

Q

W

W

F

F

G

G

C

H

Q

Q

H

H

M

Y

G

G

V

I

Q

K

P

P

D

D

L

L

I

I

T

A

F

M

A

A

K
|
K

G

G

V

L

T

S

S

S

G

G

Y

Y

F

L

P

P

L

I

G

S

G

A

V

V

I

G

V

V

G

A

E

D

R

H

V

I

A

V

E

A

G

E

L

L

I

R

E

E

Q

K

G

G

G

G

E

D

F

F

H

I

H

H

G

G

Y
x
F

T
|
T

Y

Y

S

S

G

G

H

H

P

P

G

T

G

A

C

A

A

A

A

V

A

A

L

L

A

K

T

N

L

I

K

E

I

I

M

M

H

E

E

R

E

E

D

G

L

L

V

V

A

E

R

R

V

T

K

R

C

D

E

E

I

T

G

G

P

P

Y

Y

L

L

Q

A

E

Q

R

A

W

L

L

A

P

S

L

L

A

N

N

D

H

H

P

P

L

L

V

V

G

G

E

E

A

V

R

R

M

S

V

L

G

G

L

L

I

I

G

G

A

A

L

V

E

E

L

I

T

V

P

R

H

E

K

K

E

G

T

T

R

N

A

H

K

R

F

F

P

L

G

D

E

K

V

E

G

G

T

E

V

A

G

G

L

P

I

I

A

V

R

R

D

D

I

L

S

C

F

I

R

K

E

N

G

G

L

L

V

M

M

V

R

R

A

A

V

I

R

R

D

D

S

S

L

I

I

V

L

C

A

C

P

P

P

P

F

L

T

I

L

I

S

T

E

K

E

A

E

Q

A

I

D

D

D

E

L

L

I

V

A

G

T

I

V

I

G

R

R

K

V

S

L

L

D

D

Q

E

T

A

L

E

A

P

V

V

V

L

R

R

binding pyridoxal-5'-phosphate: S116 (≠ G120), G117 (= G121), S118 (= S122), Y150 (= Y154), H151 (= H155), G152 (= G156), E223 (= E227), D256 (= D260), V258 (= V262), K285 (= K289), F318 (≠ Y322), T319 (= T323)

5ti8B Crystal structure of an aspartate aminotransferase from pseudomonas (see paper)
53% identity, 90% coverage: 35:454/465 of query aligns to 1:382/384 of 5ti8B

query
sites
5ti8B

R

R

V

I

I

I

T

T

R

K

A

A

E

E

G

G

V

V

Y

Y

I

L

F

W

D

D

S

S

E

E

G

G

N

N

R

K

I

I

L

L

D

D

G

G

M

M

S

A

G

G

L

L

W

W

C

C

S

M

A

N

V

V

G

G

H

Y

G

G

R

R

H

K

A

E

I

L

V

A

D

E

A

V

I

A

A

Y

D

K

Q

Q

L

M

R

L

Q

E

L

L

D

P

Y

Y

Y

Y

N

N

T

L

F

F

F

F

K

Q

T

T

T

A

H

H

P

P

G

P

A

A

A

L

E

E

L

L

A

A

A

K

A

A

I

I

A
|
A

Q

D

V

I

A

A

P

P

E