SitesBLAST
Comparing WP_043917276.1 NCBI__GCF_000877395.1:WP_043917276.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P02910 Histidine-binding periplasmic protein; HBP from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
39% identity, 92% coverage: 22:252/252 of query aligns to 28:260/260 of P02910
- E40 (≠ A35) mutation to K: Decrease in ATPase-inducing activity and histidine transport; when associated with K-47.
- K42 (= K37) mutation to E: Increases ATPase-inducing activity and histidine transport; when associated with K-47.
- E47 (≠ N42) mutation to K: Decrease in ATPase-inducing activity and histidine transport; when associated with K-40. Increases ATPase-inducing activity and histidine transport; when associated with E-42.
- D171 (= D165) mutation D->A,N: Strong decrease in ATPase-inducing activity and histidine transport.
- R176 (= R170) mutation R->D,S: Strong decrease in ATPase-inducing activity and histidine transport.
- D178 (= D172) mutation to A: Slight decrease in ATPase-inducing activity and histidine transport.
Sites not aligning to the query:
1hslA Refined 1.89 angstroms structure of the histidine-binding protein complexed with histidine and its relationship with many other active transport(slash)chemosensory receptors (see paper)
39% identity, 92% coverage: 22:252/252 of query aligns to 6:238/238 of 1hslA
P0AEU0 Histidine-binding periplasmic protein; HBP from Escherichia coli (strain K12) (see 3 papers)
38% identity, 92% coverage: 21:252/252 of query aligns to 27:260/260 of P0AEU0
- C60 (= C55) modified: Disulfide link with 67
- C67 (= C62) modified: Disulfide link with 60
- S91 (≠ A86) binding L-histidine
- S92 (= S87) binding L-histidine
- S94 (= S89) binding L-histidine
- R99 (= R94) binding L-histidine
- T143 (≠ I138) binding L-histidine
- D183 (= D177) binding L-histidine
Sites not aligning to the query:
P02911 Lysine/arginine/ornithine-binding periplasmic protein; LAO-binding protein from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
37% identity, 99% coverage: 2:251/252 of query aligns to 3:259/260 of P02911
- D33 (≠ S27) binding L-arginine; binding L-lysine; binding L-ornithine; mutation to A: Decreases arginine and histidine binding affinity.
- Y36 (= Y31) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
- D52 (≠ E47) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
- C60 (= C55) modified: Disulfide link with 67
- C67 (= C62) modified: Disulfide link with 60
- F74 (≠ W69) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
- S91 (≠ A86) binding L-arginine; binding L-ornithine; mutation to A: Has no effect on the arginine and histidine binding affinity.
- S92 (= S87) binding L-arginine; binding L-lysine; binding L-ornithine; mutation to A: Decreases arginine and histidine binding affinity.
- S94 (= S89) binding L-arginine; binding L-lysine; binding L-ornithine; mutation to A: Decreases arginine and histidine binding affinity.
- R99 (= R94) binding L-arginine; binding L-lysine; binding L-ornithine; mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
- L139 (≠ Q134) mutation to K: Changes ligand specificity. Can bind glutamine. Still able to bind basic amino acids, however, with 1000-fold less affinity for arginine.; mutation to Q: Does not affect ligand preference.
- T143 (≠ I138) binding L-arginine; binding L-lysine; binding L-ornithine
- D183 (= D177) binding L-arginine; binding L-ornithine; mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
5owfA Structure of a lao-binding protein mutant with glutamine (see paper)
38% identity, 91% coverage: 22:251/252 of query aligns to 3:234/235 of 5owfA
1lstA Three-dimensional structures of the periplasmic lysine-, arginine-, ornithine-binding protein with and without a ligand (see paper)
38% identity, 91% coverage: 22:251/252 of query aligns to 6:237/238 of 1lstA
1lahE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
38% identity, 91% coverage: 22:251/252 of query aligns to 6:237/238 of 1lahE
1lagE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
38% identity, 91% coverage: 22:251/252 of query aligns to 6:237/238 of 1lagE
- binding histidine: Y14 (= Y31), F52 (≠ W69), S69 (≠ A86), S70 (= S87), L71 (≠ M88), S72 (= S89), R77 (= R94), L117 (≠ Q134), S120 (≠ T137), Q122 (≠ H139), D161 (= D177)
1lafE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
38% identity, 91% coverage: 22:251/252 of query aligns to 6:237/238 of 1lafE
- binding arginine: D11 (≠ S27), Y14 (= Y31), F52 (≠ W69), S69 (≠ A86), S70 (= S87), S72 (= S89), R77 (= R94), L117 (≠ Q134), S120 (≠ T137), T121 (≠ I138), Q122 (≠ H139), D161 (= D177)
8hqrA Crystal structure of the arginine-/lysine-binding protein sar11_1210 from 'candidatus pelagibacter ubique' htcc1062 bound to arginine
37% identity, 90% coverage: 22:248/252 of query aligns to 10:264/267 of 8hqrA
- binding arginine: E15 (≠ S27), Y18 (= Y31), W56 (= W69), A73 (= A86), G74 (≠ S87), S76 (= S89), R81 (= R94), E94 (≠ T107), Q143 (= Q134), T146 (= T137), I147 (= I138)
4zv1A An ancestral arginine-binding protein bound to arginine (see paper)
35% identity, 89% coverage: 22:245/252 of query aligns to 6:225/226 of 4zv1A
- binding arginine: E11 (≠ S27), F14 (≠ Y31), F52 (≠ W69), A69 (= A86), G70 (≠ S87), M71 (= M88), T72 (≠ S89), R77 (= R94), Q117 (= Q134), S120 (≠ T137), T121 (≠ I138), D161 (= D177)
4zv2A An ancestral arginine-binding protein bound to glutamine (see paper)
35% identity, 89% coverage: 22:245/252 of query aligns to 6:223/225 of 4zv2A
3tqlA Structure of the amino acid abc transporter, periplasmic amino acid- binding protein from coxiella burnetii. (see paper)
33% identity, 86% coverage: 30:245/252 of query aligns to 11:225/225 of 3tqlA
Sites not aligning to the query:
5orgA Structure of the periplasmic binding protein (pbp) occj from a. Tumefaciens b6 in complex with octopine. (see paper)
33% identity, 87% coverage: 31:249/252 of query aligns to 14:254/257 of 5orgA
- binding octopine: Y14 (= Y31), W52 (= W69), A69 (= A86), A70 (≠ S87), M71 (= M88), S72 (= S89), R77 (= R94), N92 (= N106), Q140 (= Q134), T143 (= T137), T144 (≠ I138), N183 (≠ D177)
Sites not aligning to the query:
5otcA Structure of the periplasmic binding protein (pbp) noct from agrobacterium tumefaciens c58 in complex with noroctopinic acid. (see paper)
35% identity, 89% coverage: 24:248/252 of query aligns to 4:251/256 of 5otcA
- binding (2~{S})-5-azanyl-2-(2-hydroxy-2-oxoethylamino)pentanoic acid: E9 (= E29), Y12 (= Y31), W50 (= W69), A68 (≠ S87), G70 (≠ S89), R75 (= R94), Q138 (= Q134), T141 (= T137), S142 (≠ I138), H143 (= H139), S180 (≠ F181)
5otaA Structure of the periplasmic binding protein (pbp) noct from agrobacterium tumefaciens c58 in complex with octopinic acid (see paper)
35% identity, 89% coverage: 24:248/252 of query aligns to 4:251/254 of 5otaA
- binding (2~{S})-5-azanyl-2-[[(2~{R})-1-oxidanyl-1-oxidanylidene-propan-2-yl]amino]pentanoic acid: E9 (= E29), Y12 (= Y31), W50 (= W69), A68 (≠ S87), G70 (≠ S89), R75 (= R94), M90 (≠ A109), Q138 (= Q134), T141 (= T137), S142 (≠ I138), H143 (= H139), S180 (≠ F181)
5ot9A Structure of the periplasmic binding protein (pbp) noct from a.Tumefaciens c58 in complex with histopine. (see paper)
35% identity, 89% coverage: 24:248/252 of query aligns to 4:251/254 of 5ot9A
- binding Histopine: E9 (= E29), Y12 (= Y31), W50 (= W69), A67 (= A86), A68 (≠ S87), R75 (= R94), M90 (≠ A109), Q138 (= Q134), T141 (= T137), S142 (≠ I138), H143 (= H139), S180 (≠ F181)
4powA Structure of the pbp noct in complex with pyronopaline (see paper)
35% identity, 89% coverage: 24:248/252 of query aligns to 4:251/254 of 4powA
- binding 1-[(1S)-4-carbamimidamido-1-carboxybutyl]-5-oxo-D-proline: E9 (= E29), Y12 (= Y31), W50 (= W69), A67 (= A86), A68 (≠ S87), G70 (≠ S89), R75 (= R94), M90 (≠ A109), Q138 (= Q134), T141 (= T137), S142 (≠ I138), H143 (= H139), S180 (≠ F181)
5ovzA High resolution structure of the pbp noct in complex with nopaline (see paper)
33% identity, 89% coverage: 24:248/252 of query aligns to 5:252/259 of 5ovzA