SitesBLAST

C38 (= C42) mutation to S: No activity.
T40 (= T44) mutation to A: No activity.; mutation to S: Little decrease in activity.
H43 (= H47) mutation to A: No activity.; mutation to R: Higher level of activity at pH 9.

1rjwA Crystal structure of NAD(+)-dependent alcohol dehydrogenase from bacillus stearothermophilus strain lld-r (see paper)
56% identity, 99% coverage: 5:340/340 of query aligns to 1:336/339 of 1rjwA

query
sites
1rjwA

M

M

K

K

A

A

V

V

T

E

D

Q

F

F

S

K

K

E

P

P

L

L

E

K

I

I

K

K

E

E

V

V

E

E

R

K

P

P

T

T

V

I

S

S

D

Y

G

G

K

E

I

V

L

L

V

V

K

R

I

I

E

K

A

A

C

C

G

G

V

V

C
|
C

H
|
H

T
|
T

D

D

L

L

H
|
H

A

A

R

H

G

G

D

D

W

W

P

P

V

V

K

K

P

P

E

K

P

L

P

P

F

L

I

I

P

P

G

G

H
|
H

E
|
E

G

G

V

V

G

G

I

I

V

V

A

E

E

E

V

V

G

G

R

P

G

G

V

V

A

T

S

H

V

L

K

K

E

V

G

G

D

D

R

R

V

V

G

G

V

I

P

P

W

W

L

L

H

Y

H

S

A

A

C
|
C

G

G

H

H

C
|
C

T

D

A

Y

C
|
C

V

L

T

S

G

G

W

Q

E

E

T

T

L

L

C
|
C

R

E

T

H

E

Q

P

-

Q
x
K

Y

N

T

A

G

G

Y

Y

T

S

V

V

N

D

G

G

F

Y

A

A

E

E

Y

Y

V

C

E

R

A

A

D

A

P

A

A

D

Y

Y

V

V

G

V

H

K

L

I

P

P

G

D

A

N

L

L

D

S

F

F

A

E

P

E

A

A

P

P

I

I

L

F

C
|
C

A

A

G

G

V

V

T
|
T

V

T

Y

Y

K

K

G

A

L

L

K

K

E

V

C

T

D

G

L

A

R

K

P

P

G

G

Q

E

S

W

V

V

V

A

I

I

S

Y

G

G

I

I

G

G

L

L

G

G

H

H

L

V

A

A

V

V

Q

Q

Y

Y

A

A

R

K

A

A

M

M

G

G

L

L

H

N

V

V

I

V

A

A

V

V

D

D

I

I

A

G

E

D

D

E

K

K

L

L

R

E

L

L

A

A

R

K

D

E

L

L

G

G

A

A

E

D

T

L

T

V

I

V

N

N

A

P

G

L

D

K

T

E

D

D

P

A

V

A

D

K

E

F

V

M

T

K

-

E

R

K

L

V

G

G

A

V

D

H

G

A

V

A

L

V

V

V

T

T

A

A

V

V

S

S

N

K

S

P

A

A

F

F

T

Q

Q

S

A

A

V

Y

G

N

M

S

L

I

A

R

P

R

G

G

T

A

M

C

S

V

L

L

V

V

G

G

L

L

P

P

G

E

D

E

F

M

S

P

L

I

N

P

I

I

F

F

D

D

V

T

V

V

L

L

N

N

R

G

K

I

T

K

I

I

R

I

G

G

S

S

I
|
I

V

V

G

G

T

T

R

R

A

K

D

D

L

L

A

Q

E

E

S

A

L

L

S

Q

F

F

A

A

E

E

G

G

T

K

V

V

A

K

S

T

H

I

Y

I

A

E

T

V

D

Q

R

P

L

L

D

E

N

K

I

I

N

N

G

E

I

V

F

F

E

D

A

R

M

M

E

L

Q

K

G

G

K

Q

I

I

D

N

G

G

R
|
R

I

V

V

V

M

L

E

T

M

L

active site: C38 (= C42), H39 (= H43), T40 (= T44), H43 (= H47), H61 (= H65), E62 (= E66), C92 (= C96), C95 (= C99), C98 (= C102), C106 (= C110), K110 (≠ Q115), C148 (= C153), T152 (= T157), R331 (= R335)
binding trifluoroethanol: T40 (= T44), C148 (= C153), I285 (= I289)
binding zinc ion: C38 (= C42), H61 (= H65), C92 (= C96), C95 (= C99), C98 (= C102), C106 (= C110)

3piiA Crystal structure of mutant of ht- alcohol dehydrogenase with substrate analogue butyramide
56% identity, 99% coverage: 5:340/340 of query aligns to 1:336/337 of 3piiA

query
sites
3piiA

M

M

K

K

A

A

V

V

T

E

D

Q

F

F

S

K

K

E

P

P

L

L

E

K

I

I

K

K

E

E

V

V

E

E

R

K

P

P

T

T

V

I

S

S

D

Y

G

G

K

E

I

V

L

L

V

V

K

R

I

I

E

K

A

A

C

C

G

G

V

V

C
|
C

H
|
H

T
|
T

D

D

L

L

H
|
H

A

A

R

H

G

G

D

D

W

W

P

P

V

V

K

K

P

P

E

K

P

L

P

P

F

L

I

I

P

P

G

G

H
|
H

E
|
E

G

G

V

V

G

G

I

I

V

V

A

E

E

E

V

V

G

G

R

P

G

G

V

V

A

T

S

H

V

L

K

K

E

V

G

G

D

D

R

R

V

V

G

G

V

I

P

P

W
|
W

L

L

H

Y

H

S

A

A

C
|
C

G

G

H

H

C
|
C

T

D

A

Y

C
|
C

V

L

T

S

G

G

W

Q

E

E

T

T

L

L

C
|
C

R

E

T

H

E

Q

P

-

Q
x
K

Y

N

T

A

G

G

Y

Y

T

S

V

V

N

D

G

G

F

Y

A

A

E

E

Y

Y

V

C

E

R

A

A

D

A

P

A

A

D

Y

Y

V

V

G

V

H

K

L

I

P

P

G

D

A

N

L

L

D

S

F

F

A

E

P

E

A

A

P

P

I

I

L

F

C
|
C

A

A

G

G

V

V

T
|
T

V

T

Y

Y

K

K

G

A

L

L

K

K

E

V

C

T

D

G

L

A

R

K

P

P

G

G

Q

E

S

W

V

V

V

A

I

I

S

Y

G

G

I

I

G

G

L

F

G

G

H

H

L

V

A

A

V

V

Q

Q

Y

Y

A

A

R

K

A

A

M

M

G

G

L

L

H

N

V

V

I

V

A

A

V

V

D

D

I

I

A

G

E

D

D

E

K

K

L

L

R

E

L

L

A

A

R

K

D

E

L

L

G

G

A

A

E

D

T

L

T

V

I

V

N

N

A

P

G

L

D

K

T

E

D

D

P

A

V

A

D

K

E

F

V

M

T

K

-

E

R

K

L

V

G

G

A

V

D

H

G

A

V

A

L

V

V

V

T

T

A

A

V

V

S

S

N

K

S

P

A

A

F

F

T

Q

Q

S

A

A

V

Y

G

N

M

S

L

I

A

R

P

R

G

G

T

A

M

C

S

V

L

L

V

V

G

G

L

L

P

P

G

E

D

E

F

M

S

P

L

I

N

P

I

I

F

F

D

D

V

T

V

V

L

L

N

N

R

G

K

I

T

K

I

I

R

I

G

G

S

S

I

I

V

V

G

G

T

T

R

R

A

K

D

D

L

L

A

Q

E

E

S

A

L

L

S

Q

F

F

A

A

E

E

G

G

T

K

V

V

A

K

S

T

H

I

Y

I

A

E

T

V

D

Q

R

P

L

L

D

E

N

K

I

I

N

N

G

E

I

V

F

F

E

D

A

R

M

M

E

L

Q

K

G

G

K

Q

I

I

D

N

G

G

R
|
R

I

V

V

V

M

L

E

T

M

L

active site: C38 (= C42), H39 (= H43), T40 (= T44), H43 (= H47), H61 (= H65), E62 (= E66), C92 (= C96), C95 (= C99), C98 (= C102), C106 (= C110), K110 (≠ Q115), C148 (= C153), T152 (= T157), R331 (= R335)
binding butyramide: T40 (= T44), H61 (= H65), W87 (= W91), C148 (= C153)
binding zinc ion: C38 (= C42), H61 (= H65), E62 (= E66), C92 (= C96), C95 (= C99), C98 (= C102), C106 (= C110), C148 (= C153)

4eezB Crystal structure of lactococcus lactis alcohol dehydrogenase variant re1 (see paper)
46% identity, 99% coverage: 5:340/340 of query aligns to 1:338/342 of 4eezB

query
sites
4eezB

M

M

K

K

A

A

V

V

T

R

-

H

D

N

F

P

S

D

K

G

P

Y

L

A

E

D

I

L

K

V

E

E

V

K

E

E

R

L

P

R

T

A

V

I

S

K

D

P

G

N

K

E

I

A

L

L

V

L

K

D

I

M

E

E

A

Y

C

C

G

G

V

V

C
|
C

H
|
H

T
|
T

D

D

L

L

H
|
H

A

V

A

A

R

A

G

G

D

D

W

F

P

G

V

N

K

K

P

A

E

G

P

T

P

-

F

-

I

V

P

L

G

G

H
|
H

E
|
E

G

G

V

I

G

G

I

I

V

V

A

K

E

E

V

I

G

G

R

A

G

D

V

V

A

S

S

S

V

L

K

Q

E

V

G

G

D

D

R

R

V

V

G

S

V

V

P

A

W

W

L

F

H

F

H

E

A

G

C
|
C

G

G

H

H

C
|
C

T

E

A

Y

C
|
C

V

V

T

S

G

G

W

N

E

E

T

T

L

F

C
|
C

R

R

T

-

E

E

P

V

Q
x
K

Y

N

T

A

G

G

Y

Y

T

S

V

V

N

D

G

G

F

M

A

A

E

E

Y

E

V

A

E

I

A

V

D

V

P

A

A

D

Y

Y

V

A

G

V

H

K

L

V

P

P

G

D

A

G

L

L

D

D

F

P

A

I

P

E

A

A

A

S

P

S

I

I

L

T

C
|
C

A

A

G

G

V

V

T
|
T

V

T

Y

Y

K

K

G

A

L

I

K

K

E

V

C

S

D

G

L

V

R

K

P

P

G

G

Q

D

S

W

V

Q

V

V

I

I

S

F

G

G

I

A

G

G

L

L

G

G

H

N

L

L

A

A

V

I

Q

Q

Y

Y

A

A

R

K

-

N

A

V

M

F

G

G

L

A

H

K

V

V

I

I

A

A

V

V

D

D

I

I

A

N

E

Q

D

D

K

K

L

L

R

N

L

L

A

A

R

K

D

K

L

I

G

G

A

A

E

D

T

V

T

T

I

I

N

N

A

S

G

G

D

D

T

V

D

N

P

P

V

V

D

D

E

E

V

I

T

K

R

K

L

I

G

T

G

G

A

G

D

L

G

G

V

V

-

Q

-

S

-

A

L

I

V

V

T

C

A

A

V

V

S

A

N

R

S

I

A

A

F

F

T

E

Q

Q

A

A

V

V

G

A

M

S

L

L

A

K

P

P

G

M

G

G

T

K

M

M

S

V

L

A

V

V

G

A

L

V

P

P

P

N

G

T

D

E

F

M

S

T

L

L

N

S

I

V

F

P

D

T

V

V

L

F

N

D

R

G

K

V

T

E

I

V

R

A

G

G

S

S

I

L

V

V

G

G

T

T

R

R

A

L

D

D

L

L

A

A

E

E

S

A

L

F

S

Q

F

F

A

G

A

A

E

E

G

G

T

K

V

V

A

K

S

P

H

I

Y

V

A

A

T

T

D

R

R

K

L

L

D

E

N

E

I

I

N

N

G

D

I

I

F

I

E

D

A

E

M

M

E

K

Q

A

G

G

K

K

I

I

D

E

G

G

R
|
R

I

M

V

V

M

I

E

D

M

F

active site: C39 (= C42), H40 (= H43), T41 (= T44), H44 (= H47), H60 (= H65), E61 (= E66), C91 (= C96), C94 (= C99), C97 (= C102), C105 (= C110), K109 (≠ Q115), C147 (= C153), T151 (= T157), R333 (= R335)
binding zinc ion: C39 (= C42), H60 (= H65), E61 (= E66), C91 (= C96), C94 (= C99), C97 (= C102), C105 (= C110), C147 (= C153)

P00331 Alcohol dehydrogenase 2; Alcohol dehydrogenase II; ADHII; YADH-2; EC 1.1.1.1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
41% identity, 100% coverage: 1:339/340 of query aligns to 3:345/348 of P00331

query
sites
P00331

M

I

P

P

S

E

T

T

M

Q

K

K

A

A

A

I

V

I

V

F

T

Y

D

E

F

S

S

N

K

G

P

K

L

L

E

E

I

H

K

K

E

D

V

I

E

P

R

V

P

P

T

K

V

P

S

K

D

P

G

N

K

E

I

L

L

L

V

I

K

N

I

V

E

K

A

Y

C

S

G

G

V

V

C

C

H

H

T

T

D

D

L

L

H

H

A

A

A

W

R

H

G

G

D

D

W

W

P

P

V

L

K

P

P

T

E

K

P

L

P

P

F

L

I

V

P

G

G

G

H

H

E

E

G

G

V

A

G

G

I

V

V

V

A

V

E

G

V

M

G

G

R

E

G

N

V

V

A

K

S

G

V

W

K

K

E

I

G

G

D

D

R

Y

V

A

G

G

V

I

P

K

W

W

L

L

H

N

H

G

A

S

C

C

G

M

H

A

C

C

T

E

A

Y

C

C

V

E

T

L

G

G

W

N

E

E

T

S

L

N

C

C

R

-

T

P

E

H

P

A

Q

D

Y

L

T

S

G

G

Y

Y

T

T

V

H

N

D

G

G

G

S

F

F

A

Q

E

E

Y

Y

V

A

E

T

A

A

D

D

P

A

A

V

Y

Q

V

A

G

A

H

H

L

I

P

P

G

Q

A

G

L

T

D

D

F

L

A

A

P

E

A

V

A

A

P

P

I

I

L

L

C

C

A

A

G

G

V

I

T

T

V

V

Y

Y

K

K

G

A

L

L

K

K

E

S

C

A

D

N

L

L

R

R

P

A

G

G

Q

H

S

W

V

A

I

I

S

S

G

G

-

A

I

A

G

G

L

L

G

G

H

S

L

L

A

A

V

V

Q

Q

Y

Y

A

A

R

K

A

A

M

M

G

G

L

Y

H

R

V

V

I

L

A

G

V

I

D

D

I

G

A

G

E

P

D

G

K

K

L

E

R

E

L

L

A

F

R

T

D

S

L

L

G

G

A

G

E

E

T

V

T

F

I

I

N

D

-

F

A

T

G

K

D

E

T

K

D

D

P

I

V

V

D

S

E

A

V

V

T

V

R

K

L

A

-

T

-

N

G

G

A

A

D

H

G

G

V

I

L

I

V

N

T

V

A

S

V

V

S

S

N

E

S

A

A

A

F

I

T

E

Q

A

A

S

V

T

G

R

M

Y

L

C

A

R

P

A

G

N

G

G

T

T

M

V

S

V

L

L

V

V

G

G

L

L

P

P

P

A

G

G

-

A

D

K

F

C

S

S

L

S

N

D

I

V

F

F

D

N

V

H

V

V

L

V

N

K

R

S

K

I

T

S

I

I

R

V

G

G

S

S

I

Y

V

V

G

G

T

N

R

R

A

A

D

D

L

T

A

R

E

E

S

A

L

L

S

D

F

F

A

F

A

A

E

R

G

G

T

L

V

V

A

K

S

S

H

P

Y

I

A

K

T

V

D

V

R

G

L

L

D

S

N

S

I

L

N

P

G

E

I

I

F

Y

E

E

A

K

M

M

E

E

Q

K

G

G

K

Q

I

I

D

A

G

G

R

R

I

Y

V

V

M

V

E

D

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylserine

4gkvB Structure of escherichia coli adhp (ethanol-inducible dehydrogenase) with bound NAD (see paper)
46% identity, 99% coverage: 5:340/340 of query aligns to 1:334/336 of 4gkvB

query
sites
4gkvB

M

M

K

K

A

A

V

V

T

T

D

K

F

-

S

D

K

H

P

H

L

V

E

D

I

V

K

T

E

Y

V

K

E

T

R

L

P

R

T

S

V

L

S

K

D

H

G

G

K

E

I

A

L

L

V

L

K

K

I

M

E

E

A

C

C

C

G

G

V

V

C
|
C

H
|
H

T
|
T

D

D

L

L

H
|
H

A

V

A

K

R

N

G

G

D

D

W

F

P

G

V

D

K

K

P

T

E

-

P

-

P

G

F

V

I

I

P

L

G

G

H
|
H

E
|
E

G

G

V

I

G

G

I

V

V

V

A

A

E

E

V

V

G

G

R

P

G

G

V

V

A

T

S

S

V

L

K

K

E

P

G

G

D

D

R

R

V

A

G

S

V

V

P

A

W

W

L

F

H

Y

H

E

A

G

C
|
C

G

G

H

H

C
|
C

T

E

A

Y

C
|
C

V

N

T

S

G

G

W

N

E

E

T

T

L

L

C
|
C

R

R

T

S

E

-

P

V

Q
x
K

Y

N

T

A

G

G

Y

Y

T

S

V

V

N

D

G

G

F

M

A

A

E

E

Y

E

V

C

E

I

A

V

D

V

P

A

A

D

Y

Y

V

A

G

V

H

K

L

V

P

P

G

D

A

G

L

L

D

D

F

S

A

A

P

A

A

A

A

S

P

S

I

I

L

T

C
|
C

A

A

G

G

V

V

T
|
T

V

T

Y

Y

K

K

G

A

L

V

K

K

E

L

C

S

D

K

L

I

R

R

P

P

G

G

Q

Q

S

W

V

I

V

A

I

I

S

Y

G
|
G

I

L

G
|
G

L
|
L

G

G

H

N

L

L

A

A

V

L

Q

Q

Y

Y

A

A

R

K

-

N

A

V

M

F

G

N

L

A

H

K

V

V

I

I

A

A

V

I

D
|
D

I
x
V

A

N

E

D

D

E

K
x
Q

L

L

R

K

L

L

A

A

R

T

D

E

L

M

G

G

A

A

E

D

T

L

T

A

I

I

N

N

A

S

G

H

D

T

T

E

D

D

P

A

V

A

D

K

E

I

V

V

T
x
Q

-

E

R

K

L

T

G

G

A

A

D

H

G

A

V

A

L

V

V

V

T
|
T

A
|
A

V
|
V

S

A

N

K

S

A

A

A

F

F

T

N

Q

S

A

A

V

V

G
x
D

M

A

L

V

A

R

P

A

G

G

T

R

M

V

S

V

L

A

V
|
V

G
|
G

L
|
L

P

P

G

E

D

S

F

M

S

S

L

L

N

D

I

I

F

P

D
x
R

V

L

V

V

L
|
L

N

D

R

G

K

I

T

E

I

V

R

V

G

G

S

S

I
x
L

V
|
V

G

G

T

T

R

R

A

Q

D

D

L

L

A

T

E

E

S

A

L

F

S

Q

F

F

A

A

E

E

G

G

T

K

V

V

A

V

S

P

H

K

Y

V

A

A

T

L

D

R

R

P

L

L

D

A

N

D

I

I

N

N

G

T

I

I

F

F

E

T

A

E

M

M

E

E

Q

E

G

G

K

K

I

I

D

R

G

G

R
|
R

I

M

V

V

M

I

E

D

M

F

active site: C37 (= C42), H38 (= H43), T39 (= T44), H42 (= H47), H58 (= H65), E59 (= E66), C89 (= C96), C92 (= C99), C95 (= C102), C103 (= C110), K107 (≠ Q115), C145 (= C153), T149 (= T157), R329 (= R335)
binding nicotinamide-adenine-dinucleotide: C37 (= C42), H38 (= H43), T39 (= T44), H42 (= H47), C145 (= C153), T149 (= T157), G169 (= G177), G171 (= G179), G172 (= G180), L173 (= L181), D193 (= D200), V194 (≠ I201), Q198 (≠ K205), T235 (= T241), A236 (= A242), V237 (= V243), V258 (= V264), G259 (= G265), L260 (= L266), L283 (≠ I289), V284 (= V290), R329 (= R335)
binding zinc ion: C37 (= C42), H58 (= H65), C89 (= C96), C92 (= C99), C95 (= C102), C103 (= C110), C145 (= C153)
binding : Q223 (≠ T230), D247 (≠ G253), R271 (≠ D277), L274 (= L280)

5envA Yeast alcohol dehydrogenase with bound coenzyme (see paper)
41% identity, 100% coverage: 1:339/340 of query aligns to 2:344/347 of 5envA

query
sites
5envA

M

I

P

P

S

E

T

T

M

Q

K

K

A

G

A

V

V

I

V

F

T

Y

D

E

F

S

S

H

K

G

P

K

L

L

E

E

I

Y

K

K

E

D

V

I

E

P

R

V

P

P

T

K

V

P

S

K

D

A

G

N

K

E

I

L

L

L

V

I

K

N

I

V

E

K

A

Y

C

S

G

G

V

V

C
|
C

H
|
H

T
|
T

D

D

L

L

H
|
H

A

A

A

W

R

H

G

G

D

D

W
|
W

P

P

V

L

K

P

P

V

E

K

P

L

P

P

F

L

I

V

P

G

G

G

H
|
H

E
|
E

G

G

V

A

G

G

I

V

V

V

A

V

E

G

V

M

G

G

R

E

G

N

V

V

A

K

S

G

V

W

K

K

E

I

G

G

D

D

R

Y

V

A

G

G

V

I

P

K

W
|
W

L

L

H

N

H

G

A

S

C
|
C

G

M

H

A

C
|
C

T

E

A

Y

C
|
C

V

E

T

L

G

G

W

N

E

E

T

S

L

N

C
|
C

R

-

T

P

E

H

P

A

Q
x
D

Y

L

T

S

G

G

Y

Y

T

T

V

H

N

D

G

G

G

S

F

F

A

Q

E

Q

Y

Y

V

A

E

T

A

A

D

D

P

A

A

V

Y

Q

V

A

G

A

H

H

L

I

P

P

G

Q

A

G

L

T

D

D

F

L

A

A

P

Q

A

V

A

A

P

P

I

I

L

L

C
|
C

A

A

G

G

V

I

T
|
T

V

V

Y

Y

K

K

G

A

L

L

K

K

E

S

C

A

D

N

L

L

R

M

P

A

G

G

Q

H

S

W

V

V

V

A

I

I

S

S

G
|
G

-

A

I

A

G
|
G

L
|
L

G

G

H

S

L

L

A

A

V

V

Q

Q

Y

Y

A

A

R

K

A

A

M

M

G

G

L

Y

H

R

V

V

I

L

A

G

V

I

D
|
D

I

G

A

G

E

E

D

G

K
|
K

L

E

R

E

L

L

A

F

R

R

D

S

L

I

G

G

A

G

E

E

T

V

T

F

I

I

N

D

-
x
F

A

T

G

K

D

E

T

K

D

D

P

I

V

V

D

G

E

A

V

V

T

L

R

K

L

A

-

T

-

D

G

G

A

A

D

H

G

G

V

V

L

I

V

N

T

V

A
x
S

V

V

S

S

N

E

S

A

A

A

F

I

T

E

Q

A

A

S

V

T

G

R

M

Y

L

V

A

R

P

A

G

N

G

G

T

T

M

T

S

V

L

L

V
|
V

G
|
G

L
x
M

P

P

P

A

G

G

-

A

D

K

F

C

S

C

L

S

N

D

I

V

F

F

D

N

V

Q

V

V

L

V

N

K

R

S

K

I

T

S

I

I

R

V

G

G

S

S

I
x
Y

V
|
V

G

G

T

N

R

R

A

A

D

D

L

T

A

R

E

E

S

A

L

L

S

D

F

F

A

F

A

A

E

R

G

G

T

L

V

V

A

K

S

S

H

P

Y

I

A

K

T

V

D

V

R

G

L

L

D

S

N

T

I

L

N

P

G

E

I

I

F

Y

E

E

A

K

M

M

E

E

Q

K

G

G

K

Q

I

I

D

V

G

G

R
|
R

I

Y

V

V

M

V

E

D

active site: C43 (= C42), H44 (= H43), T45 (= T44), H48 (= H47), H66 (= H65), E67 (= E66), C97 (= C96), C100 (= C99), C103 (= C102), C111 (= C110), D115 (≠ Q115), C153 (= C153), R340 (= R335)
binding trifluoroethanol: T45 (= T44), W54 (= W53), H66 (= H65), W92 (= W91), C153 (= C153), M270 (≠ L266), Y294 (≠ I289)
binding nicotinamide-adenine-dinucleotide: H44 (= H43), T45 (= T44), H48 (= H47), T157 (= T157), G177 (= G177), G180 (= G179), G181 (= G180), L182 (= L181), D201 (= D200), K206 (= K205), F221 (vs. gap), S246 (≠ A242), V268 (= V264), G269 (= G265), V295 (= V290)
binding zinc ion: C43 (= C42), H66 (= H65), C97 (= C96), C100 (= C99), C103 (= C102), C111 (= C110), C153 (= C153)

4w6zA Yeast alcohol dehydrogenase i, saccharomyces cerevisiae fermentative enzyme (see paper)
41% identity, 100% coverage: 1:339/340 of query aligns to 2:344/347 of 4w6zA

query
sites
4w6zA

M

I

P

P

S

E

T

T

M

Q

K

K

A

G

A

V

V

I

V

F

T

Y

D

E

F

S

S

H

K

G

P

K

L

L

E

E

I

Y

K

K

E

D

V

I

E

P

R

V

P

P

T

K

V

P

S

K

D

A

G

N

K

E

I

L

L

L

V

I

K

N

I

V

E

K

A

Y

C

S

G

G

V

V

C
|
C

H
|
H

T
|
T

D

D

L

L

H
|
H

A

A

A

W

R

H

G

G

D

D

W
|
W

P

P

V

L

K

P

P

V

E

K

P

L

P

P

F

L

I

V

P

G

G

G

H
|
H

E
|
E

G

G

V

A

G

G

I

V

V

V

A

V

E

G

V

M

G

G

R

E

G

N

V

V

A

K

S

G

V

W

K

K

E

I

G

G

D

D

R

Y

V

A

G

G

V

I

P

K

W
|
W

L

L

H

N

H

G

A

S

C
|
C

G

M

H

A

C
|
C

T

E

A

Y

C
|
C

V

E

T

L

G

G

W

N

E

E

T

S

L

N

C
|
C

R

-

T

P

E

H

P

A

Q
x
D

Y

L

T

S

G

G

Y

Y

T

T

V

H

N

D

G

G

G

S

F

F

A

Q

E

Q

Y

Y

V

A

E

T

A

A

D

D

P

A

A

V

Y

Q

V

A

G

A

H

H

L

I

P

P

G

Q

A

G

L

T

D

D

F

L

A

A

P

Q

A

V

A

A

P

P

I

I

L

L

C
|
C

A

A

G

G

V

I

T
|
T

V

V

Y

Y

K

K

G

A

L

L

K

K

E

S

C

A

D

N

L

L

R

M

P

A

G

G

Q

H

S

W

V

V

V

A

I

I

S

S

G
|
G

-

A

I

A

G
|
G

L
|
L

G

G

H

S

L

L

A

A

V

V

Q

Q

Y

Y

A

A

R

K

A

A

M

M

G

G

L

Y

H

R

V

V

I

L

A

G

V
x
I

D
|
D

I

G

A

G

E

E

D

G

K
|
K

L

E

R

E

L

L

A

F

R

R

D

S

L

I

G

G

A

G

E

E

T

V

T

F

I

I

N

D

-
x
F

A

T

G

K

D

E

T

K

D

D

P

I

V

V

D

G

E

A

V

V

T

L

R

K

L

A

-

T

-

D

G

G

A

A

D

H

G

G

V

V

L

I

V

N

T

V

A
x
S

V

V

S
|
S

N

E

S

A

A
|
A

F

I

T

E

Q

A

A

S

V

T

G

R

M

Y

L

V

A

R

P

A

G

N

G

G

T

T

M

T

S

V

L

L

V
|
V

G
|
G

L
x
M

P

P

P

A

G

G

-

A

D

K

F

C

S

C

L

S

N

D

I

V

F

F

D

N

V

Q

V

V

L

V

N

K

R

S

K

I

T

S

I

I

R

V

G

G

S
|
S

I
x
Y

V
|
V

G

G

T

N

R

R

A

A

D

D

L

T

A

R

E

E

S

A

L

L

S

D

F

F

A

F

A

A

E

R

G

G

T

L

V

V

A

K

S

S

H

P

Y

I

A

K

T

V

D

V

R

G

L

L

D

S

N

T

I

L

N

P

G

E

I

I

F

Y

E

E

A

K

M

M

E

E

Q

K

G

G

K

Q

I

I

D

V

G

G

R
|
R

I

Y

V

V

M

V

E

D

active site: C43 (= C42), H44 (= H43), T45 (= T44), H48 (= H47), H66 (= H65), E67 (= E66), C97 (= C96), C100 (= C99), C103 (= C102), C111 (= C110), D115 (≠ Q115), C153 (= C153), R340 (= R335)
binding nicotinamide-8-iodo-adenine-dinucleotide: C43 (= C42), H44 (= H43), T45 (= T44), H48 (= H47), W54 (= W53), C153 (= C153), T157 (= T157), G177 (= G177), G180 (= G179), G181 (= G180), L182 (= L181), I200 (≠ V199), D201 (= D200), K206 (= K205), F221 (vs. gap), S246 (≠ A242), S248 (= S244), A251 (= A247), V268 (= V264), G269 (= G265), M270 (≠ L266), S293 (= S288), Y294 (≠ I289), V295 (= V290), R340 (= R335)
binding trifluoroethanol: T45 (= T44), W54 (= W53), H66 (= H65), W92 (= W91), C153 (= C153)
binding zinc ion: C43 (= C42), H66 (= H65), C97 (= C96), C100 (= C99), C103 (= C102), C111 (= C110), C153 (= C153)

P00330 Alcohol dehydrogenase 1; Alcohol dehydrogenase I; ADHI; NADH-dependent methylglyoxal reductase; YADH-1; EC 1.1.1.1; EC 1.1.1.54; EC 1.1.1.78 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 6 papers)
41% identity, 100% coverage: 1:339/340 of query aligns to 3:345/348 of P00330

query
sites
P00330

M

I

P

P

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E

T

T

M

Q

K

K

A

G

A

V

V

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L

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K

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K

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K

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A

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C

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C

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H

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H
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H

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W

R

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W
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W

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L

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M

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M

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F

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G

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M

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R

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L

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S

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F

F

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V

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K

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S

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C44 (= C42) binding Zn(2+)
H45 (= H43) binding NAD(+); mutation to R: Decreases dissociation constants by 4-fold for NAD(+) and 2-fold for NADH, while turnover numbers were decreased by 4-fold for ethanol oxidation and 6-fold for acetaldehyde reduction.
T46 (= T44) binding NAD(+); mutation to S: Has the same pattern of activity as the wild-type enzyme for linear primary alcohols.
H49 (= H47) binding NAD(+)
W55 (= W53) mutation to M: Has lowered reactivity with primary and secondary alcohols.
H67 (= H65) binding Zn(2+)
E68 (= E66) binding in the open conformation
W93 (= W91) mutation to A: Has an inverted specificity pattern for primary alcohols, being 3- and 10-fold more active on hexanol and 350- and 540-fold less active on ethanol. Also acquires weak activity on branched chain alcohols and cyclohexanol.
C98 (= C96) binding Zn(2+)
C101 (= C99) binding Zn(2+)
C104 (= C102) binding Zn(2+)
C112 (= C110) binding Zn(2+)
C154 (= C153) binding Zn(2+)
G181 (= G179) binding NAD(+)
G182 (= G180) binding NAD(+)
L183 (= L181) binding NAD(+)
D202 (= D200) binding NAD(+)
K207 (= K205) binding NAD(+)
F222 (vs. gap) binding NAD(+)
T236 (vs. gap) natural variant: T -> I
V269 (= V264) binding NAD(+)
M271 (≠ L266) binding NAD(+); mutation to L: Produces a 7 to 10-fold increase in reactivity with butanol, pentanol, and hexanol.
S294 (= S288) binding NAD(+)
V296 (= V290) binding NAD(+)
R341 (= R335) binding NAD(+)

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylserine

Query Sequence

>WP_043917880.1 NCBI__GCF_000877395.1:WP_043917880.1
MPSTMKAAVVTDFSKPLEIKEVERPTVSDGKILVKIEACGVCHTDLHAARGDWPVKPEPP
FIPGHEGVGIVAEVGRGVASVKEGDRVGVPWLHHACGHCTACVTGWETLCRTEPQYTGYT
VNGGFAEYVEADPAYVGHLPGALDFAPAAPILCAGVTVYKGLKECDLRPGQSVVISGIGG
LGHLAVQYARAMGLHVIAVDIAEDKLRLARDLGAETTINAGDTDPVDEVTRLGGADGVLV
TAVSNSAFTQAVGMLAPGGTMSLVGLPPGDFSLNIFDVVLNRKTIRGSIVGTRADLAESL
SFAAEGTVASHYATDRLDNINGIFEAMEQGKIDGRIVMEM

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory