SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_043919947.1 NCBI__GCF_000877395.1:WP_043919947.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 19 hits to proteins with known functional sites (download)

3e5zA X-ray structure of the putative gluconolactonase in protein family pf08450. Northeast structural genomics consortium target drr130.
41% identity, 97% coverage: 9:304/306 of query aligns to 7:289/290 of 3e5zA

query
sites
3e5zA

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binding magnesium ion: E30 (= E32), N117 (= N126), N174 (= N183), D220 (= D235)

3dr2A Structural and functional analyses of xc5397 from xanthomonas campestris: a gluconolactonase important in glucose secondary metabolic pathways (see paper)
41% identity, 92% coverage: 16:298/306 of query aligns to 29:298/299 of 3dr2A

query
sites
3dr2A

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binding calcium ion: E45 (= E32), N131 (= N126), N188 (= N183), D236 (= D235)

Q9A9Z1 D-xylonolactone lactonase; Xylono-1,5-lactonase; EC 3.1.1.110 from Caulobacter vibrioides (strain ATCC 19089 / CIP 103742 / CB 15) (Caulobacter crescentus) (see paper)
31% identity, 84% coverage: 32:288/306 of query aligns to 17:251/289 of Q9A9Z1

query
sites
Q9A9Z1

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E17 (= E32) binding Fe(2+)
N145 (= N183) binding Fe(2+)
D195 (= D235) binding Fe(2+)

7pldB Caulobacter crescentus xylonolactonase with (r)-4-hydroxy-2- pyrrolidone (see paper)
31% identity, 84% coverage: 32:288/306 of query aligns to 17:251/289 of 7pldB

query
sites
7pldB

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binding (R)-4-hydroxy-2-pyrrolidone: E17 (= E32), I31 (= I48), A64 (= A80), G66 (= G82), F67 (≠ H83), P80 (≠ D107), R98 (≠ S124), N100 (= N126), E119 (= E155), D138 (≠ V176), N145 (= N183), K180 (≠ G223), D195 (= D235), W210 (vs. gap), W210 (vs. gap)
binding fe (ii) ion: E17 (= E32), N145 (= N183), D195 (= D235)

Sites not aligning to the query:

binding (R)-4-hydroxy-2-pyrrolidone: 15

7plbB Caulobacter crescentus xylonolactonase with d-xylose (see paper)
31% identity, 84% coverage: 32:288/306 of query aligns to 17:251/289 of 7plbB

query
sites
7plbB

E
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T

V

V

A

D

S

A

D

Q

G

G

A
x
R

V

L

W

W

F

F

S

G

D

T

P

M

H

H

Y

D

G

G

I

-

M

-

D

-

Y

-

E

-

G

-

A

-

R

-

A

-

E

E

Q

E

E

N

N

N

P

S

C

G

V

S

V

L

Y

Y

R

R

I

M

P
x
D

P

L

E

T

G

G

G

-

V

-

P

V

E

A

P

R

M

M

V

D

T

R

D

D

M

I

N

C

C

I

P

T

N
|
N

G

G

L

P

A

C

F

V

S

S

P

P

D

D

E

G

T

K

L

T

L

F

Y

Y

V

H

A

T

D

D

T

T

G

L

R

E

M

-

W

-

S

-

N

-

D

-

P

-

Q

K

H

T

I

I

R

Y

V

A

Y

F

D

D

M

L

-

A

E

E

D
|
D

G

G

R

L

P

L

V

S

N

N

G

K

R

R

L

V

F

F

A

V

K

Q

P

F

D

A

A

L

G

G

-

D

-

V

I

Y

A

P

D
|
D

G

G

F

S

R

V

L

V

D

D

E

S

D

E

G

G

N

Y

L

L

W

W

T

T

S

A

-

L

-

W

A

G

G

G

D

F

G

G

V

A

H

V

C

R

F

F

A

S

P

P

D

Q

G

G

R

D

L

A

L

V

G

T

K

R

I

I

R

E

V

L

P

P

E

A

-

P

T

N

V

V

S

T

N

K

V

P

C

C

F

F

G

G

R

P

A

D

K

L

H

K

V

T

L

L

Y

Y

I

F

T

T

binding fe (ii) ion: E17 (= E32), N145 (= N183), D195 (= D235)
binding beta-D-xylopyranose: E17 (= E32), E89 (≠ D115), V90 (≠ R116), D92 (= D118), R98 (≠ S124), N100 (= N126), R109 (≠ A135), D130 (≠ P166), N145 (= N183), D174 (= D217), D195 (= D235)
binding alpha-D-xylopyranose: A64 (= A80), G66 (= G82), F67 (≠ H83), P80 (≠ D107)

Sites not aligning to the query:

binding beta-D-xylopyranose: 15

7risA Crystal structure of rpa3624, a beta-propeller lactonase from rhodopseudomonas palustris, with active-site bound phosphate (see paper)
26% identity, 91% coverage: 21:298/306 of query aligns to 1:279/293 of 7risA

query
sites
7risA

L

V

K

R

R

V

L

L

A

A

T

T

G

D

F

L

D

A

W

F

V

P

E
|
E

G

G

P

P

V

V

W

V

F

M

G

P

D

D

A

-

G

G

C

S

L

V

F

L

S

V

D

E

I

I

P

R

N

A

N

Q

R

Q

I

L

L

T

R

R

W

V

T

W

P

P

D

D

R

-

G

-

T

-

G

G

P

R

D

K

E

E

L

V

G

-

V

-

T

-

T

V

H

A

R

K

A

V

P

P

S

G

N

G

Y

-

A

P

N

N

G

G

H

A

T

A

R

L

D

G

R

P

Q

D

G

G

R

K

L

M

V

Y

S

I

C

C

E

N

H

N

G

G

A

G

-

F

-

G

-

P

-

G

-

A

-

P

-

A

-

P

-

H

-

E

-

Y

-

I

-

G

R

G

R

S

V

I

T

Q

R

R

T

V

E

D

-

L

H

Q

D

S

G

G

T

E

I

V

T

E

V

T

L

L

A

F

D

D

R

K

F

C

D

G

G

E

K

H

P

P

L

L

N

K

S

G

P

P

N
|
N

D

D

V

L

I

V

V

F

A

D

S

K

D

H

G

G

A

G

V

L

W

W

F

F

S

T

D

-

P

-

H

-

Y

-

G

-

I

-

M

-

D

D

Y

L

E

G

G

K

A

R

R

R

A

A

E

R

Q

D

E

M

N

D

P

V

C

G

V

A

Y

Y

R

Y

I

I

P

K

P

P

E

G

G

M

G

T

V

E

P

I

E

T

P

E

M

Q

V

V

T

F

D

G

M

T

N

L

C

P

P

L

N
|
N

G

G

L

I

A

G

F

L

S

S

P

P

D

D

E

E

T

A

L

T

L

M

Y

Y

V

A

A

A

D

E

-

T

-

P

T

T

G

G

R

R

M

L

W

W

S

A

N

F

D

D

-

L

-

S

-

G

P

P

Q

G

H

E

I

V

R

K

V

P

Y

R

D

D

M

G

E

E

D

K

G

G

R

K

P

P

V

I

N

C

G

G

-

L

-

G

-

Y

R

Q

L

M

F

F

A

-

K

-

P

-

D

-

A

-

G

-

I

-

A

-

D
|
D

G
x
S

F

L

R

A

L

V

D

E

E

A

D

S

G

G

N

N

L

V

W

C

-

V

-

A

T

T

S

L

A

V

G

S

D

G

G

C

V

I

H

S

C

V

F

I

A

A

P

P

D

D

G

G

R

T

L

L

L

V

G

E

K

Q

I

V

R

P

V

T

P

G

E

D

T

R

V

V

-

T

S
x
T

N

N

V

I

C

A

F

F

G

G

R

P

A

D

K

L

H

K

V

T

L

A

Y

Y

I

I

T

T

A

L

T

S

T

G

S

K

L

G

Y

E

S

L

V

I

A

A

L

M

binding calcium ion: E12 (= E32), N111 (= N126), N160 (= N183), D213 (= D235), S214 (≠ G236)
binding phosphate ion: E12 (= E32), N111 (= N126), N160 (= N183), D213 (= D235), T254 (≠ S273)

7rizA Crystal structure of rpa3624, a beta-propeller lactonase from rhodopseudomonas palustris, with active-site bound 2-hydroxyquinoline (see paper)
25% identity, 91% coverage: 21:298/306 of query aligns to 1:292/306 of 7rizA

query
sites
7rizA

L

V

K

R

R

V

L

L

A

A

T

T

G

D

F

L

D

A

W

F

V

P

E
|
E

G

G

P

P

V

V

W

V

F

M

G

P

D

D

A

-

G

G

C

S

L

V

F

L

S

V

D

E

I

I

P

R

N

A

N

Q

R

Q

I

L

L

T

R

R

W

V

T

W

P

P

D

D

R

-

G

-

T

-

G

G

P

R

D

K

E

E

L

V

G

-

V

-

T

-

T

V

H

A

R

K

A

V

P

P

S

G

N

G

Y

-

A

P

N
|
N

G

G

H

A

T

A

R

L

D

G

R

P

Q

D

G

G

R

K

L

M

V

Y

S

I

C

C

E

N

H

N

G

G

A

G

-
x
F

-

G

-

W

-

F

-

P

-

S

-

R

-

G

-

T

-

M

-
x
P

-

G

-

A

-

P

-

A

-

P

-

H

-

E

-

Y

-

I

-

G

R

G

R

S

V

I

T

Q

R

R

T

V

E

D

-

L

H

Q

D

S

G

G

T

E

I

V

T

E

V

T

L

L

A

F

D

D

R

K

F

C

D

G

G

E

K

H

P

P

L

L

N

K

S

G

P

P

N
|
N

D

D

V

L

I

V

V

F

A

D

S

K

D

H

G

G

A

G

V

L

W

W

F

F

S

T

D

-

P

-

H

-

Y

-

G

-

I

-

M

-

D

D

Y

L

E

G

G

K

A

R

R

R

A

A

E

R

Q

D

E

M

N

D

P

V

C

G

V

A

Y

Y

R

Y

I

I

P

K

P

P

E

G

G

M

G

T

V

E

P

I

E

T

P

E

M

Q

V

V

T

F

D

G

M

T

N

L

C

P

P

L

N
|
N

G

G

L

I

A

G

F

L

S

S

P

P

D

D

E

E

T

A

L

T

L

M

Y

Y

V

A

A

A

D

E

-

T

-

P

T

T

G

G

R

R

M

L

W

W

S

A

N

F

D

D

-

L

-

S

-

G

P

P

Q

G

H

E

I

V

R

K

-

P

-

R

-

D

-

V

V

I

Y

Y

D

R

M

G

E

E

D

K

G

G

R

K

P

P

V

I

N

C

G

G

R

L

L

G

F

G

A

Y

K

Q

P

-

D

-

A

-

G

-

I

M

A

F

D
|
D

G
x
S

F

L

R

A

L

V

D

E

E

A

D

S

G

G

N

N

L

V

W

C

-

V

-

A

T

T

S

L

A

V

G

S

D

G

G

C

V

I

H

S

C

V

F

I

A

A

P

P

D

D

G

G

R

T

L

L

L

V

G

E

K

Q

I

V

R

P

V

T

P

G

E

D

T

R

V

V

-

T

S

T

N

N

V

I

C

A

F

F

G

G

R

P

A

D

K

L

H

K

V

T

L

A

Y

Y

I

I

T

T

A

L

T

S

T

G

S

K

L

G

Y

E

S

L

V

I

A

A

L

M

binding calcium ion: E12 (= E32), N120 (= N126), N169 (= N183), D226 (= D235), S227 (≠ G236)
binding quinolin-2(1h)-one: E12 (= E32), N52 (= N81), F70 (vs. gap), P81 (vs. gap), N120 (= N126), D226 (= D235)

8dk0A Crystal structure of rpa3624, a beta-propeller lactonase from rhodopseudomonas palustris, with active-site bound (s)gamma- valerolactone (see paper)
25% identity, 91% coverage: 21:298/306 of query aligns to 3:279/293 of 8dk0A

query
sites
8dk0A

L

V

K

R

R

V

L

L

A

A

T

T

G

D

F

L

D

A

W
x
F

V

P

E
|
E

G

G

P

P

V

V

W

V

F

M

G

P

D

D

A

-

G

G

C

S

L

V

F

L

S

V

D

E

I

I

P

R

N

A

N

Q

R

Q

I

L

L

T

R

R

W

V

T

W

P

P

D

D

R

-

G

-

T

-

G

G

P

R

D

K

E

E

L

V

G

-

V

-

T

-

T

V

H

A

R

K

A

V

P

P

S

G

N

G

Y

-

A

P

N

N

G

G

H

A

T

A

R

L

D

G

R

P

Q

D

G

G

R

K

L

M

V

Y

S

I

C

C

E

N

H

N

G

G

A

G

-
x
F

-

G

-

W

-

M

-

P

-

G

-

A

-

P

-

A

-

P

-

H

-

E

-

Y

-

I

-

G

R

G

R

S

V

I

T

Q

R

R

T

V

E

D

-

L

H

Q

D

S

G

G

T

E

I

V

T

E

V

T

L

L

A

F

D

D

R

K

F

C

D

G

G

E

K

H

P

P

L

L

N

K

S

G

P

P

N
|
N

D

D

V

L

I

V

V

F

A

D

S

K

D

H

G

G

A

G

V

L

W

W

F

F

S

T

D

-

P

-

H

-

Y

-

G

-

I

-

M

-

D

D

Y

L

E

G

G

K

A

R

R

R

A

A

E

R

Q

D

E

M

N

D

P

V

C

G

V

A

Y

Y

R

Y

I

I

P

K

P

P

E

G

G

M

G

T

V

E

P

I

E

T

P

E

M

Q

V

V

T

F

D

G

M

T

N

L

C

P

P

L

N
|
N

G

G

L

I

A

G

F

L

S

S

P

P

D

D

E

E

T

A

L

T

L

M

Y

Y

V

A

A

A

D

E

-

T

-

P

T

T

G

G

R

R

M

L

W

W

S

A

N

F

D

D

-

L

-

S

-

G

P

P

Q

G

H

E

I

V

R

K

V

-

Y

-

D

-

M

-

E

P

D

K

G

G

R

K

P

P

V

I

N

C

G

G

-

L

-

G

-

Y

R

Q

L

M

F

F

A

-

K

-

P

-

D

-

A

-

G

-

I

-

A

-

D
|
D

G
x
S

F

L

R

A

L

V

D

E

E

A

D

S

G

G

N

N

L

V

W

C

-

V

-

A

T

T

S

L

A

V

G

S

D

G

G

C

V

I

H

S

C

V

F

I

A

A

P

P

D

D

G

G

R

T

L

L

L

V

G

E

K

Q

I

V

R

P

V

T

P

G

E

D

T

R

V

V

-

T

S
x
T

N

N

V

I

C

A

F

F

G

G

R

P

A

D

K

L

H

K

V

T

L

A

Y

Y

I

I

T

T

A

L

T

S

T

G

S

K

L

G

Y

E

S

L

V

I

A

A

L

M

binding calcium ion: E14 (= E32), N115 (= N126), N164 (= N183), D213 (= D235), S214 (≠ G236)
binding gamma-Valerolactone: F12 (≠ W30), E14 (= E32), F72 (vs. gap), D213 (= D235), T254 (≠ S273)

8djzA Crystal structure of rpa3624, a beta-propeller lactonase from rhodopseudomonas palustris, with active-site bound product (see paper)
25% identity, 91% coverage: 21:298/306 of query aligns to 3:279/293 of 8djzA

query
sites
8djzA

L

V

K

R

R

V

L

L

A

A

T

T

G

D

F

L

D

A

W

F

V

P

E
|
E

G

G

P

P

V

V

W

V

F

M

G

P

D

D

A

-

G

G

C

S

L

V

F

L

S

V

D

E

I

I

P

R

N

A

N

Q

R

Q

I

L

L

T

R

R

W

V

T

W

P

P

D

D

R

-

G

-

T

-

G

G

P

R

D

K

E

E

L

V

G

-

V

-

T

-

T

V

H

A

R

K

A

V

P

P

S

G

N

G

Y

-

A

P

N

N

G

G

H

A

T

A

R

L

D

G

R

P

Q

D

G

G

R

K

L

M

V

Y

S

I

C

C

E

N

H

N

G

G

A

G

-

F

-

G

-

W

-

M

-

P

-

G

-

A

-

P

-

A

-

P

-

H

-

E

-

Y

-

I

-

G

R

G

R

S

V

I

T

Q

R

R

T

V

E

D

-

L

H

Q

D

S

G

G

T

E

I

V

T

E

V

T

L

L

A

F

D

D

R

K

F

C

D

G

G

E

K

H

P

P

L

L

N

K

S

G

P

P

N
|
N

D

D

V

L

I

V

V

F

A

D

S

K

D

H

G

G

A

G

V

L

W

W

F

F

S

T

D

-

P

-

H

-

Y

-

G

-

I

-

M

-

D

D

Y

L

E

G

G

K

A

R

R

R

A

A

E

R

Q

D

E

M

N

D

P

V

C

G

V

A

Y

Y

R

Y

I

I

P

K

P

P

E

G

G

M

G

T

V

E

P

I

E

T

P

E

M

Q

V

V

T

F

D

G

M

T

N

L

C

P

P

L

N
|
N

G

G

L

I

A

G

F

L

S

S

P

P

D

D

E

E

T

A

L

T

L

M

Y

Y

V

A

A

A

D

E

-

T

-

P

T

T

G

G

R

R

M

L

W

W

S

A

N

F

D

D

-

L

-

S

-

G

P

P

Q

G

H

E

I

V

R

K

V

-

Y

-

D

-

M

-

E

P

D

K

G

G

R

K

P

P

V

I

N

C

G

G

-

L

-

G

-

Y

R

Q

L

M

F

F

A

-

K

-

P

-

D

-

A

-

G

-

I

-

A

-

D
|
D

G
x
S

F

L

R

A

L

V

D

E

E

A

D

S

G

G

N

N

L

V

W

C

-

V

-

A

T

T

S

L

A

V

G

S

D

G

G

C

V

I

H

S

C

V

F

I

A

A

P

P

D

D

G

G

R

T

L

L

L

V

G

E

K

Q

I

V

R

P

V

T

P

G

E

D

T

R

V

V

-

T

S

T

N

N

V

I

C

A

F

F

G

G

R

P

A

D

K

L

H

K

V

T

L

A

Y

Y

I

I

T

T

A

L

T

S

T

G

S

K

L

G

Y

E

S

L

V

I

A

A

L

M

binding calcium ion: E14 (= E32), N115 (= N126), N164 (= N183), D213 (= D235), S214 (≠ G236)
binding (4R)-4-hydroxypentanoic acid: E14 (= E32), N115 (= N126), D213 (= D235)

8djfA Crystal structure of rpa3624, a beta-propeller lactonase from rhodopseudomonas palustris, with active-site bound tetrahedral intermediate (see paper)
25% identity, 91% coverage: 21:298/306 of query aligns to 3:279/293 of 8djfA

query
sites
8djfA

L

V

K

R

R

V

L

L

A

A

T

T

G

D

F

L

D

A

W

F

V

P

E
|
E

G

G

P

P

V

V

W

V

F

M

G

P

D

D

A

-

G

G

C

S

L

V

F

L

S

V

D

E

I

I

P

R

N

A

N

Q

R

Q

I

L

L

T

R

R

W

V

T

W

P

P

D

D

R

-

G

-

T

-

G

G

P

R

D

K

E

E

L

V

G

-

V

-

T

-

T

V

H

A

R

K

A

V

P

P

S

G

N

G

Y

-

A

P

N
|
N

G

G

H

A

T

A

R

L

D

G

R

P

Q

D

G

G

R

K

L

M

V

Y

S

I

C

C

E

N

H

N

G

G

A

G

-
x
F

-

G

-

W

-

M

-

P

-

G

-

A

-

P

-

A

-

P

-

H

-

E

-

Y

-

I

-

G

R

G

R

S

V

I

T

Q

R

R

T

V

E

D

-

L

H

Q

D

S

G

G

T

E

I

V

T

E

V

T

L

L

A

F

D

D

R

K

F

C

D

G

G

E

K

H

P

P

L

L

N

K

S

G

P

P

N
|
N

D

D

V

L

I

V

V

F

A

D

S

K

D

H

G

G

A

G

V

L

W

W

F

F

S

T

D

-

P

-

H

-

Y

-

G

-

I

-

M

-

D

D

Y

L

E

G

G

K

A

R

R

R

A

A

E

R

Q

D

E

M

N

D

P

V

C

G

V

A

Y

Y

R

Y

I

I

P

K

P

P

E

G

G

M

G

T

V

E

P

I

E

T

P

E

M

Q

V

V

T

F

D

G

M

T

N

L

C

P

P

L

N
|
N

G

G

L

I

A

G

F

L

S

S

P

P

D

D

E

E

T

A

L

T

L

M

Y

Y

V

A

A

A

D

E

-

T

-

P

T

T

G

G

R

R

M

L

W

W

S

A

N

F

D

D

-

L

-

S

-

G

P

P

Q

G

H

E

I

V

R

K

V

-

Y

-

D

-

M

-

E

P

D

K

G

G

R

K

P

P

V

I

N

C

G

G

-

L

-

G

-

Y

R

Q

L

M

F

F

A

-

K

-

P

-

D

-

A

-

G

-

I

-

A

-

D
|
D

G
x
S

F

L

R

A

L

V

D

E

E

A

D

S

G

G

N

N

L

V

W

C

-

V

-

A

T

T

S

L

A

V

G

S

D

G

G

C

V

I

H

S

C

V

F

I

A

A

P

P

D

D

G

G

R

T

L

L

L

V

G

E

K

Q

I

V

R

P

V

T

P

G

E

D

T

R

V

V

-

T

S

T

N

N

V

I

C

A

F

F

G

G

R

P

A

D

K

L

H

K

V

T

L

A

Y

Y

I

I

T

T

A

L

T

S

T

G

S

K

L

G

Y

E

S

L

V

I

A

A

L

M

binding calcium ion: E14 (= E32), N115 (= N126), N164 (= N183), D213 (= D235), S214 (≠ G236)
binding (5S)-5-methyloxolane-2,2-diol: E14 (= E32), N54 (= N81), F72 (vs. gap), N115 (= N126), D213 (= D235)

4gnaA Mouse smp30/gnl-xylitol complex (see paper)
26% identity, 84% coverage: 32:289/306 of query aligns to 16:260/297 of 4gnaA

query
sites
4gnaA

E
|
E

G

S

P

P

V

V

W

W

F

E

G

E

D

A

A

S

G

Q

C

S

L

L

F

F

S

V

D

D

I

I

P

P

N

S

N

K

R

I

I

I

L

C

R

R

W

W

T

D

P

-

D

-

R

-

G

-

T

T

G

V

P

S

D

N

E

Q

L

V

G

Q

V

R

V

V

T

A

T

V

H

D

R

A

A

P

P

V

S

S

N

S

Y

V

A

A

N

-

G

-

H

-

T

L

R

R

D

Q

R

L

Q

G

G

G

R

Y

L

V

V

A

S

T

C

I

E

-

H

-

G

G

A

T

R

K

R

F

V

C

T

A

R

L

T

N

E

W

H

E

D

N

G

Q

T

S

I

V

T

F

V

V

L

L

A

A

D

M

R

V

F

D

D

E

G

D

K

K

P

K

L

N

N

N

S
x
R

P

F

N
|
N

D

D

V

G

I

K

V

V

A

D

S

P

D

A

G

G

A

R

V

Y

W

-

F

-

S

-

D

-

P

-

H

-

Y

-

G

-

I

-

M

-

D

-

Y

F

E

A

G

G

A

T

R

M

A

A

E

E

Q
x
E

E

T

N

A

P

P

C

A

V

V

-

L

-

E

-

R

-

H

-

Q

-

G

-

S

V

L

Y

Y

R

S

I

L

P

F

P

P

E

D

G

H

G

S

V

V

P

-

E

K

P

K

M

Y

V

F

T

D

D

Q

M

V

N

D

C

I

P

S

N
|
N

G

G

L

L

A

D

F

W

S

S

P

L

D

D

E

H

T

K

L

I

L

F

Y

Y

V

Y

A

I

D

D

T

S

G

-

R

L

M

S

W

Y

S

T

N

V

D

D

P

A

Q

F

H

D

I

-

R

-

V

-

Y

Y

D

D

M

L

E

Q

D

T

G

G

R

Q

P

I

V

S

N

N

G

R

R

R

L

I

F

V

A

Y

K

K

-

M

-

E

P

K

D

D

A

E

G

Q

I

I

A

P

D
|
D

G

G

F

M

R

C

L

I

D

D

E

A

D

E

G

G

N

K

L

L

W

W

T

V

S

A

A

C

G
x
Y

D

N

G

G

V

G

H

R

C

V

F

I

A

R

P

L

D

D

-

P

-

E

-

T

G

G

R

K

L

R

L

L

G

Q

K

T

I

V

R

K

V

L

P

P

-

V

E

D

T

K

V

T

S

T

N

S

V

C

C

C

F

F

G

G

R

K

A

D

K

Y

H

S

V

E

L

M

Y

Y

I

V

T

T

A

C

binding calcium ion: E16 (= E32), N152 (= N183), D202 (= D235)
binding Xylitol: E16 (= E32), R99 (≠ S124), N101 (= N126), E119 (≠ Q156), N152 (= N183), D202 (= D235), Y217 (≠ G250)

4gn9A Mouse smp30/gnl-glucose complex (see paper)
26% identity, 84% coverage: 32:289/306 of query aligns to 16:260/297 of 4gn9A

query
sites
4gn9A

E
|
E

G

S

P

P

V

V

W

W

F

E

G

E

D

A

A

S

G

Q

C

S

L

L

F

F

S

V

D

D

I

I

P

P

N

S

N

K

R

I

I

I

L

C

R

R

W

W

T

D

P

-

D

-

R

-

G

-

T

T

G

V

P

S

D

N

E

Q

L

V

G

Q

V

R

V

V

T

A

T

V

H

D

R

A

A

P

P

V

S

S

N

S

Y

V

A

A

N

-

G

-

H

-

T

L

R

R

D

Q

R

L

Q

G

G
|
G

R

Y

L

V

V

A

S

T

C

I

E

-

H

-

G

G

A

T

R

K

R

F

V

C

T

A

R

L

T

N

E
x
W

H
x
E

D

N

G

Q

T

S

I

V

T

F

V

V

L

L

A

A

D

M

R

V

F
x
D

D
x
E

G

D

K

K

P

K

L

N

N

N

S
x
R

P

F

N
|
N

D

D

V

G

I

K

V

V

A

D

S

P

D

A

G

G

A

R

V

Y

W

-

F

-

S

-

D

-

P

-

H

-

Y

-

G

-

I

-

M

-

D

-

Y

F

E

A

G

G

A

T

R

M

A

A

E

E

Q
x
E

E

T

N

A

P

P

C

A

V

V

-

L

-

E

-

R

-

H

-

Q

-

G

-

S

V

L

Y
|
Y

R

S

I

L

P

F

P

P

E

D

G

H

G

S

V

V

P

-

E

K

P
x
K

M

Y

V

F

T

D

D

Q

M

V

N

D

C

I

P

S

N
|
N

G

G

L

L

A

D

F

W

S

S

P

L

D

D

E

H

T

K

L

I

L

F

Y

Y

V

Y

A

I

D

D

T
x
S

G

-

R

L

M

S

W

Y

S

T

N

V

D

D

P

A

Q

F

H

D

I

-

R

-

V

-

Y

Y

D

D

M

L

E

Q

D

T

G

G

R

Q

P

I

V

S

N

N

G

R

R

R

L

I

F

V

A

Y

K

K

-

M

-

E

P

K

D

D

A

E

G

Q

I

I

A

P

D
|
D

G

G

F

M

R

C

L

I

D

D

E

A

D

E

G

G

N

K

L

L

W

W

T

V

S

A

A

C

G
x
Y

D

N

G

G

V

G

H

R

C

V

F

I

A

R

P

L

D

D

-

P

-

E

-

T

G

G

R

K

L

R

L

L

G

Q

K

T

I

V

R

K

V

L

P

P

-

V

E

D

T

K

V

T

S

T

N

S

V

C

C

C

F

F

G

G

R

K

A

D

K

Y

H

S

V

E

L

M

Y

Y

I

V

T

T

A

C

binding beta-D-glucopyranose: E16 (= E32), G66 (= G89), W80 (≠ E105), E81 (≠ H106), D92 (≠ F117), E93 (≠ D118), R99 (≠ S124), N101 (= N126), E119 (≠ Q156), Y133 (= Y163), K143 (≠ P174), N152 (= N183), S168 (≠ T199), D202 (= D235), Y217 (≠ G250)
binding calcium ion: E16 (= E32), N152 (= N183), D202 (= D235)

4gn8A Mouse smp30/gnl-1,5-ag complex (see paper)
26% identity, 84% coverage: 32:289/306 of query aligns to 16:260/297 of 4gn8A

query
sites
4gn8A

E
|
E

G

S

P

P

V

V

W

W

F

E

G

E

D

A

A

S

G

Q

C

S

L

L

F

F

S

V

D

D

I

I

P

P

N

S

N

K

R

I

I

I

L

C

R

R

W

W

T

D

P

-

D

-

R

-

G

-

T

T

G

V

P

S

D

N

E

Q

L

V

G

Q

V

R

V

V

T

A

T

V

H

D

R

A

A

P

P

V

S

S

N

S

Y

V

A

A

N

-

G

-

H

-

T

L

R

R

D
x
Q

R

L

Q

G

G

G

R

Y

L

V

V

A

S

T

C

I

E

-

H

-

G

G

A

T

R

K

R

F

V

C

T

A

R

L

T

N

E

W

H

E

D

N

G

Q

T

S

I

V

T

F

V

V

L

L

A

A

D

M

R

V

F

D

D

E

G

D

K

K

P

K

L

N

N

N

S
x
R

P

F

N
|
N

D

D

V

G

I

K

V

V

A

D

S

P

D
x
A

G
|
G

A

R

V

Y

W

-

F

-

S

-

D

-

P

-

H

-

Y

-

G

-

I

-

M

-

D

-

Y

F

E

A

G

G

A

T

R

M

A

A

E

E

Q
x
E

E

T

N

A

P
|
P

C

A

V

V

-

L

-

E

-

R

-

H

-

Q

-

G

-

S

V

L

Y

Y

R

S

I

L

P

F

P

P

E

D

G

H

G

S

V

V

P

-

E

K

P

K

M

Y

V

F

T

D

D

Q

M

V

N

D

C

I

P

S

N
|
N

G

G

L

L

A

D

F

W

S

S

P

L

D

D

E

H

T

K

L

I

L

F

Y
|
Y

V

Y

A

I

D

D

T

S

G

-

R

L

M

S

W

Y

S

T

N

V

D

D

P
x
A

Q

F

H

D

I

-

R

-

V

-

Y

Y

D
|
D

M

L

E
x
Q

D

T

G

G

R

Q

P

I

V

S

N

N

G

R

R
|
R

L

I

F
x
V

A

Y

K

K

-

M

-

E

P

K

D

D

A

E

G

Q

I

I

A

P

D
|
D

G

G

F

M

R

C

L

I

D

D

E

A

D

E

G

G

N

K

L

L

W

W

T

V

S

A

A

C

G
x
Y

D

N

G

G

V

G

H

R

C

V

F

I

A

R

P

L

D

D

-
x
P

-

E

-

T

G

G

R

K

L

R

L

L

G

Q

K

T

I

V

R

K

V

L

P

P

-

V

E

D

T

K

V

T

S

T

N

S

V

C

C

C

F

F

G

G

R

K

A

D

K

Y

H

S

V

E

L

M

Y

Y

I

V

T

T

A

C

binding 1,5-anhydro-D-glucitol: Q63 (≠ D86), R99 (≠ S124), N101 (= N126), A108 (≠ D133), G109 (= G134), E119 (≠ Q156), P122 (= P159), N152 (= N183), Y164 (= Y195), A175 (≠ P207), D179 (= D214), Q181 (≠ E216), R189 (= R224), V191 (≠ F226), D202 (= D235), Y217 (≠ G250), P227 (vs. gap)
binding calcium ion: E16 (= E32), N152 (= N183), D202 (= D235)

Sites not aligning to the query:

binding 1,5-anhydro-D-glucitol: 293, 294

4gn7A Mouse smp30/gnl (see paper)
26% identity, 84% coverage: 32:289/306 of query aligns to 16:260/297 of 4gn7A

query
sites
4gn7A

E
|
E

G

S

P

P

V

V

W

W

F

E

G

E

D

A

A

S

G

Q

C

S

L

L

F

F

S

V

D

D

I

I

P

P

N

S

N

K

R

I

I

I

L

C

R

R

W

W

T

D

P

-

D

-

R

-

G

-

T

T

G

V

P

S

D

N

E

Q

L

V

G

Q

V

R

V

V

T

A

T

V

H

D

R

A

A

P

P

V

S

S

N

S

Y

V

A

A

N

-

G

-

H

-

T

L

R

R

D

Q

R

L

Q

G

G

G

R

Y

L

V

V

A

S

T

C

I

E

-

H

-

G

G

A

T

R

K

R

F

V

C

T

A

R

L

T

N

E

W

H

E

D

N

G

Q

T

S

I

V

T

F

V

V

L

L

A

A

D

M

R

V

F

D

D

E

G

D

K

K

P

K

L

N

N

N

S

R

P

F

N

N

D

D

V

G

I

K

V

V

A

D

S

P

D

A

G

G

A

R

V

Y

W

-

F

-

S

-

D

-

P

-

H

-

Y

-

G

-

I

-

M

-

D

-

Y

F

E

A

G

G

A

T

R

M

A

A

E

E

Q

E

E

T

N

A

P

P

C

A

V

V

-

L

-

E

-

R

-

H

-

Q

-

G

-

S

V

L

Y

Y

R

S

I

L

P

F

P

P

E

D

G

H

G

S

V

V

P

-

E

K

P

K

M

Y

V

F

T

D

D

Q

M

V

N

D

C

I

P

S

N
|
N

G

G

L

L

A

D

F

W

S

S

P

L

D

D

E

H

T

K

L

I

L

F

Y

Y

V

Y

A

I

D

D

T

S

G

-

R

L

M

S

W

Y

S

T

N

V

D

D

P

A

Q

F

H

D

I

-

R

-

V

-

Y

Y

D

D

M

L

E

Q

D

T

G

G

R

Q

P

I

V

S

N

N

G

R

R

R

L

I

F

V

A

Y

K

K

-

M

-

E

P

K

D

D

A

E

G

Q

I

I

A

P

D
|
D

G

G

F

M

R

C

L

I

D

D

E

A

D

E

G

G

N

K

L

L

W

W

T

V

S

A

A

C

G

Y

D

N

G

G

V

G

H

R

C

V

F

I

A

R

P

L

D

D

-

P

-

E

-

T

G

G

R

K

L

R

L

L

G

Q

K

T

I

V

R

K

V

L

P

P

-

V

E

D

T

K

V

T

S

T

N

S

V

C

C

C

F

F

G

G

R

K

A

D

K

Y

H

S

V

E

L

M

Y

Y

I

V

T

T

A

C

binding calcium ion: E16 (= E32), N152 (= N183), D202 (= D235)

3g4hA Crystal structure of human senescence marker protein-30 (zinc bound) (see paper)
25% identity, 84% coverage: 32:289/306 of query aligns to 16:260/297 of 3g4hA

query
sites
3g4hA

E
|
E

G

S

P

P

V

V

W

W

F

E

G

E

D

V

A

S

G

N

C

S

L

L

F

F

S

V

D

D

I

I

P

P

N

A

N

K

R

K

I

V

L

C

R

R

W

W

T

D

P

-

D

-

R

-

G

-

T

S

G

F

P

T

D

K

E

Q

L

V

G

Q

V

R

V

V

T

T

T

M

H

D

R

A

A

P

P

V

S

S

N

S

Y

V

A

A

N

-

G

-

H

-

T

L

R

R

D

Q

R

S

Q

G

G

G

R

Y

L

V

V

A

S

T

C

I

E

-

H

-

G

G

A

T

R

K

R

F

V

C

T

A

R

L

T

N

E

W

H

K

D

E

G

Q

T

S

I

A

T

V

V

V

L

L

A

A

D

T

R

V

F

D

D

N

G

D

K

K

P

K

L

N

N

N

S

R

P

F

N

N

D

D

V

G

I

K

V

V

A

D

S

P

D

A

G

G

A

R

V

-

W

Y

F

F

S

A

D

-

P

-

H

-

Y

-

G

G

I

T

M

M

M

A

D

E

Y

E

E

T

G

A

A

P

R

A

A

V

E

L

Q

E

E

R

N

H

P

Q

C

G

V

A

V

L

Y

Y

R

S

I

L

P

F

P

P

E

D

G

H

V

V

P

-

E

K

P

K

M

Y

V

F

T

D

D

Q

M

V

N

D

C

I

P

S

N
|
N

G

G

L

L

A

D

F

W

S

S

P

L

D

D

E

H

T

K

L

I

L

F

Y

Y

V

Y

A

I

D

D

T

S

G

-

R

L

M

S

W

Y

S

S

N

V

D

D

P

A

Q

F

H

D

I

-

R

-

V

-

Y

Y

D

D

M

L

E

Q

D

T

G

G

R

Q

P

I

V

S

N

N

G

R

R

R

L

S

F

V

A

Y

K

K

-

L

-

E

P

K

D

E

A

E

G

Q

I

I

A

P

D
|
D

G

G

F

M

R

C

L

I

D

D

E

A

D

E

G

G

N

K

L

L

W

W

T

V

S

A

A

C

G

Y

D

N

G

G

V

G

H

R

C

V

F

I

A

R

P

L

D

D

-

P

-

V

-

T

G

G

R

K

L

R

L

L

G

Q

K

T

I

V

R

K

V

L

P

P

-

V

E

D

T

K

V

T

S

T

N

S

V

C

C

C

F

F

G

G

R

K

A

N

K

Y

H

S

V

E

L

M

Y

Y

I

V

T

T

A

C

binding zinc ion: E16 (= E32), N152 (= N183), D202 (= D235)

4gncA Human smp30/gnl-1,5-ag complex (see paper)
25% identity, 84% coverage: 32:289/306 of query aligns to 17:261/298 of 4gncA

query
sites
4gncA

E
|
E

G

S

P

P

V

V

W

W

F

E

G

E

D

V

A

S

G

N

C

S

L

L

F

F

S

V

D

D

I

I

P

P

N

A

N

K

R

K

I

V

L

C

R

R

W

W

T

D

P

-

D

-

R

-

G

-

T

S

G

F

P

T

D

K

E

Q

L

V

G

Q

V

R

V

V

T

T

T

M

H

D

R

A

A

P

P

V

S

S

N

S

Y

V

A

A

N

-

G

-

H

-

T

L

R

R

D

Q

R

S

Q

G

G

G

R

Y

L

V

V

A

S

T

C

I

E

-

H

-

G

G

A

T

R

K

R

F

V

C

T

A

R

L

T

N

E

W

H

K

D

E

G

Q

T

S

I

A

T

V

V

V

L

L

A

A

D

T

R

V

F

D

D

N

G

D

K

K

P

K

L

N

N

N

S
x
R

P

F

N
|
N

D
|
D

V

G

I

K

V

V

A

D

S

P

D

A

G

G

A

R

V

-

W

Y

F

F

S

A

D

-

P

-

H

-

Y

-

G

G

I

T

M

M

M

A

D

E

Y

E

E

T

G

A

A

P

R

A

A

V

E

L

Q

E

E

R

N

H

P

Q

C

G

V

A

V

L

Y

Y

R

S

I

L

P

F

P

P

E

D

G

H

V

V

P

-

E

K

P

K

M

Y

V

F

T

D

D

Q

M

V

N

D

C

I

P

S

N
|
N

G

G

L

L

A

D

F

W

S

S

P

L

D

D

E

H

T

K

L

I

L

F

Y

Y

V

Y

A

I

D

D

T

S

G

-

R

L

M

S

W

Y

S

S

N

V

D

D

P

A

Q

F

H

D

I

-

R

-

V

-

Y

Y

D

D

M

L

E

Q

D

T

G

G

R

Q

P

I

V

S

N

N

G

R

R

R

L

S

F

V

A

Y

K

K

-

L

-

E

P

K

D

E

A

E

G

Q

I

I

A

P

D
|
D

G

G

F

M

R

C

L

I

D

D

E

A

D

E

G

G

N

K

L

L

W

W

T

V

S

A

A

C

G

Y

D

N

G

G

V

G

H

R

C

V

F

I

A

R

P

L

D

D

-

P

-

V

-

T

G

G

R

K

L

R

L

L

G

Q

K

T

I

V

R

K

V

L

P

P

-

V

E

D

T

K

V

T

S
x
T

N

S

V

C

C

C

F

F

G

G

R

K

A
x
N

K
x
Y

H

S

V

E

L

M

Y

Y

I

V

T

T

A

C

binding 1,5-anhydro-D-glucitol: E17 (= E32), R100 (≠ S124), N102 (= N126), D203 (= D235), T245 (≠ S273), N253 (≠ A281), Y254 (≠ K282)
binding calcium ion: E17 (= E32), D103 (= D127), N153 (= N183), D203 (= D235)

Sites not aligning to the query:

binding 1,5-anhydro-D-glucitol: 290, 291

Q15493 Regucalcin; RC; Gluconolactonase; GNL; Senescence marker protein 30; SMP-30; EC 3.1.1.17 from Homo sapiens (Human) (see 2 papers)
25% identity, 84% coverage: 32:289/306 of query aligns to 18:262/299 of Q15493

query
sites
Q15493

E
|
E

G

S

P

P

V

V

W

W

F

E

G

E

D

V

A

S

G

N

C

S

L

L

F

F

S

V

D

D

I

I

P

P

N

A

N

K

R

K

I

V

L

C

R

R

W

W

T

D

P

-

D

-

R

-

G

-

T

S

G

F

P

T

D

K

E

Q

L

V

G

Q

V

R

V

V

T

T

T

M

H

D

R

A

A

P

P

V

S

S

N

S

Y

V

A

A

N

-

G

-

H

-

T

L

R

R

D

Q

R

S

Q

G

G

G

R

Y

L

V

V

A

S

T

C

I

E

-

H

-

G

G

A

T

R

K

R

F

V

C

T

A

R

L

T

N

E

W

H

K

D

E

G

Q

T

S

I

A

T

V

V

V

L

L

A

A

D

T

R

V

F

D

D

N

G

D

K

K

P

K

L

N

N

N

S

R

P

F

N
|
N

D

D

V

G

I

K

V

V

A

D

S

P

D

A

G

G

A

R

V

-

W

Y

F

F

S

A

D

-

P

-

H

-

Y

-

G

G

I

T

M

M

M

A

D

E

Y

E

E

T

G

A

A

P

R

A

A

V

E

L

Q

E

E

R

N

H

P

Q

C

G

V

A

V

L

Y

Y

R

S

I

L

P

F

P

P

E

D

G

H

V

V

P

-

E

K

P

K

M

Y

V

F

T

D

D

Q

M

V

N

D

C

I

P

S

N
|
N

G

G

L

L

A

D

F

W

S

S

P

L

D

D

E

H

T

K

L

I

L

F

Y

Y

V

Y

A

I

D

D

T

S

G

-

R

L

M

S

W

Y

S

S

N

V

D

D

P

A

Q

F

H

D

I

-

R

-

V

-

Y

Y

D

D

M

L

E

Q

D

T

G

G

R

Q

P

I

V

S

N

N

G

R

R

R

L

S

F

V

A

Y

K

K

-

L

-

E

P

K

D

E

A

E

G

Q

I

I

A

P

D
|
D

G

G

F

M

R

C

L

I

D

D

E

A

D

E

G

G

N

K

L

L

W

W

T

V

S

A

A

C

G

Y

D

N

G

G

V

G

H

R

C

V

F

I

A

R

P

L

D

D

-

P

-

V

-

T

G

G

R

K

L

R

L

L

G

Q

K

T

I

V

R

K

V

L

P

P

-

V

E

D

T

K

V

T

S

T

N

S

V

C

C

C

F

F

G

G

R

K

A

N

K

Y

H

S

V

E

L

M

Y

Y

I

V

T

T

A

C

E18 (= E32) binding a divalent metal cation; mutation to A: Reduces enzyme activity by about 90%.
N103 (= N126) mutation to A: Reduces enzyme activity by about 95%.
N154 (= N183) binding a divalent metal cation; mutation to A: Reduces enzyme activity by about 95%.
D204 (= D235) binding a divalent metal cation; mutation to A: Reduces enzyme activity by over 98%.

5gx1A Luciferin-regenerating enzyme collected with serial synchrotron rotational crystallography with accumulated dose of 1.1 mgy (1st measurement) (see paper)
28% identity, 37% coverage: 177:289/306 of query aligns to 153:270/307 of 5gx1A

query
sites
5gx1A

T

S

D

N

M

I

N

A

C

I

P

A

N
|
N

G

G

L

L

A

A

F

W

S

S

P

N

D

D

E

L

T

K

L

K

L

M

Y

Y

V

Y

A

I

D

D

T

S

G

G

R

K

M

R

W

R

S

V

N

D

D

E

P

Y

Q

D

H

Y

-

D

I

A

R

S

V

T

Y

L

D

S

M

I

E

S

D

N

G

Q

R

R

P

P

V

L

N

-

G

-

R

F

L

T

F

F

A

E

K

K

P

H

D

E

A

V

-

P

G

G

I

Y

A

P

D
|
D

G

G

F

Q

R

T

L

I

D

D

E

E

D

E

G

G

N

N

L

L

W

W

T

V

S

A

A

V

G

F

D

Q

G

G

V

Q

H

R

C

I

F

I

A

K

P

I

D

S

G

T

R

Q

-

Q

-

P

-

E

-

V

L

L

G

D

K

T

I

V

R

K

V

I

P

P

E

D

-

P

T

Q

V

V

S

T

N

S

V

V

C

A

F

F

G

G

R

P

A

N

K

L

H

D

V

E

L

L

Y

Y

I

V

T

T

A

S

binding magnesium ion: N159 (= N183), D211 (= D235)

Sites not aligning to the query:

5d9bA Luciferin-regenerating enzyme solved by siras using xfel (refined against native data) (see paper)
28% identity, 37% coverage: 177:289/306 of query aligns to 153:270/307 of 5d9bA

query
sites
5d9bA

T

S

D

N

M

I

N

A

C

I

P

A

N
|
N

G

G

L

L

A

A

F

W

S

S

P

N

D

D

E

L

T

K

L

K

L

M

Y

Y

V

Y

A

I

D

D

T

S

G

G

R

K

M

R

W

R

S

V

N

D

D

E

P

Y

Q

D

H

Y

-

D

I

A

R

S

V

T

Y

L

D

S

M

I

E

S

D

N

G

Q

R

R

P

P

V

L

N

-

G

-

R

F

L

T

F

F

A

E

K

K

P

H

D

E

A

V

-

P

G

G

I

Y

A

P

D
|
D

G

G

F

Q

R

T

L

I

D

D

E

E

D

E

G

G

N

N

L

L

W

W

T

V

S

A

A

V

G

F

D

Q

G

G

V

Q

H

R

C

I

F

I

A

K

P

I

D

S

G

T

R

Q

-

Q

-

P

-

E

-

V

L

L

G

D

K

T

I

V

R

K

V

I

P

P

E

D

-

P

T

Q

V

V

S

T

N

S

V

V

C

A

F

F

G

G

R

P

A

N

K

L

H

D

V

E

L

L

Y

Y

I

V

T

T

A

S

binding magnesium ion: N159 (= N183), D211 (= D235)

Sites not aligning to the query:

binding magnesium ion: 17

Query Sequence

>WP_043919947.1 NCBI__GCF_000877395.1:WP_043919947.1
MSDFEIHDPAFARYVLGNAPLKRLATGFDWVEGPVWFGDAGCLLFSDIPNNRILRWTPDR
GTGPDELGVVTTHRAPSNYANGHTRDRQGRLVSCEHGARRVTRTEHDGTITVLADRFDGK
PLNSPNDVIVASDGAVWFSDPHYGIMMDYEGARAEQENPCVVYRIPPEGGVPEPMVTDMN
CPNGLAFSPDETLLYVADTGRMWSNDPQHIRVYDMEDGRPVNGRLFAKPDAGIADGFRLD
EDGNLWTSAGDGVHCFAPDGRLLGKIRVPETVSNVCFGGRAKHVLYITATTSLYSVALAR
RGVQRP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory