SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_044050603.1 NCBI__GCF_000738435.1:WP_044050603.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0DX97 Deoxyribokinase; dRK; ATP:2-deoxy-D-ribose 5-phosphotransferase; EC 2.7.1.229 from Salmonella typhi (see paper)
37% identity, 99% coverage: 3:304/306 of query aligns to 1:302/306 of P0DX97

query
sites
P0DX97

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M10 (≠ V12) Important for substrate specificity; mutation to N: 2.5-fold decrease in Vmax for deoxyribokinase activity and 70-fold increase in KM for deoxyribose. 2-fold increase in KM for ribose.

8cqxA Ribokinase from t.Sp mutant a92g
37% identity, 98% coverage: 5:304/306 of query aligns to 2:298/300 of 8cqxA

query
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8cqxA

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binding adenosine-5'-diphosphate: N179 (= N185), T217 (= T221), G219 (= G223), A220 (≠ E224), G222 (= G226), F250 (= F256), N272 (≠ T278), G275 (≠ A281), A276 (= A282), T279 (≠ V285)
binding magnesium ion: D242 (≠ E248), T244 (= T250), A278 (≠ S284), S287 (= S293)

4xckA Vibrio cholerae o395 ribokinase complexed with adp, ribose and cesium ion. (see paper)
39% identity, 98% coverage: 5:304/306 of query aligns to 4:302/306 of 4xckA

query
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4xckA

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active site: A249 (≠ G251), A250 (= A252), G251 (= G253), D252 (= D254)
binding adenosine-5'-diphosphate: T220 (= T221), G222 (= G223), S223 (≠ E224), V242 (≠ A243), T247 (= T249), A250 (= A252), F254 (= F256), H276 (≠ T278), A279 (= A281), V283 (= V285)
binding alpha-D-ribofuranose: N11 (≠ V12), D13 (= D14), G39 (= G40), K40 (= K41), N43 (= N44), A95 (= A95), I107 (= I108), I109 (≠ V110), E140 (= E141), T248 (= T250), D252 (= D254)

1rk2A E. Coli ribokinase complexed with ribose and adp, solved in space group p212121 (see paper)
39% identity, 98% coverage: 5:304/306 of query aligns to 4:302/305 of 1rk2A

query
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1rk2A

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W

R

R

K

E

E

E

I

I

E

D

S

A

L

F

L

L

active site: A249 (≠ G251), A250 (= A252), G251 (= G253), D252 (= D254)
binding adenosine-5'-diphosphate: T220 (= T221), G222 (= G223), S223 (≠ E224), A250 (= A252), G251 (= G253), H276 (≠ T278), A279 (= A281)
binding tetrafluoroaluminate ion: G213 (= G214), R215 (≠ G216)
binding magnesium ion: D246 (≠ E248), A282 (≠ S284), R285 (= R287), S291 (= S293)
binding alpha-D-ribofuranose: N11 (≠ V12), D13 (= D14), G38 (= G39), G39 (= G40), K40 (= K41), N43 (= N44), E140 (= E141), D252 (= D254)

P0A9J6 Ribokinase; RK; EC 2.7.1.15 from Escherichia coli (strain K12) (see 3 papers)
39% identity, 98% coverage: 5:304/306 of query aligns to 7:305/309 of P0A9J6

query
sites
P0A9J6

I

L

V

V

I

V

L

L

G

G

V

S

F

I

V
x
N

A
|
A

D
|
D

T

H

A

I

Y

L

R

N

A

L

Q

Q

R

S

Q

F

P

P

R

T

L

P

G

G

E

E

T

T

I

V

L

T

G

G

Q

N

S

H

F

Y

A

Q

L

V

G

A

P

F

G

G

G
|
G

K
|
K

G
|
G

S
x
A

N
|
N

Q

Q

S

A

V

V

A

A

A

A

G

G

M

R

A

S

G

G

A

A

S

N

V

I

G

A

F

F

I

I

T

A

K

C

L

T

G

G

K

D

D

D

P

S

F

I

A

G

D

E

-

S

-

V

-

R

M

Q

A

Q

L

L

A

A

T

T

W

-

K

D

K

N

A

I

G

D

V

I

T

T

S

P

L

V

A

S

T

V

R

I

H

K

A

G

D

E

D

S

Y

-

T

T

G

G

A

V

A

A

Y

L

I

I

F

F

V

V

D

N

E

-

N

G

T

E

G

G

D

E

N

N

A

V

I

I

I

G

V

I

C

H

P

A

G

G

A

A

A

N

A

A

T

A

I

L

N

S

R

P

S

A

D

L

I

V

D

E

S

A

H

Q

A

R

E

E

V

R

I

I

K

A

Q

N

C

A

K

S

V

A

L

L

M

L

T

M

Q

Q

L

L

E
|
E

Q

S

P

P

I

L

D

E

A

S

A

V

E

M

Y

A

A

A

L

A

A

K

C

I

A

A

R

H

K

Q

A

N

G

K

A

T

T

I

T

V

I

A

L

L

N

N

P

P

A

A

P

P

A

A

V

R

E

E

L

L

P

P

T

D

S

E

M

L

I

L

A

A

L

L

C

V

D

D

F

I

V

I

T

T

P

P

N
|
N

E

E

I

T

E

E

A

A

E

E

T

K

I

L

T

T

G

G

I

I

T

R

V

V

N

E

S

N

P

D

K

E

D

D

A

A

V

A

A

K

A

A

A

A

E

Q

A

V

L

L

L

H

A

E

K

K

G

G

A

I

G

R

G

T

A

V

I

L

I

I

T
|
T

L
|
L

G
|
G

E
x
S

N
x
R

G
|
G

C

V

L

W

Y

A

H

S

D

V

G

N

S

G

N

E

S

G

V

Q

H

R

V

V

P

P

A

G

I

F

N

R

A

V

G

-

P

Q

V

A

V

V

E

D

T

T

T

I

G

A

A

A

G
|
G

D
|
D

A

T

F

F

N

N

G

G

G

A

F

L

A

I

V

T

A

A

I

L

A

L

E

E

G

E

H

K

S

P

P

L

L

P

E

E

A

A

I

I

R

R

F

F

G

A

T
x
H

A

A

T

A

A

A

A

A

I

I

S

A

V

V

T

T

R

R

P

K

G

G

T

A

A

Q

P

P

S

S

M

V

P

P

T

W

R

R

K

E

E

E

I

I

E

D

S

A

L

F

L

L

NAD 14:16 (≠ VAD 12:14) binding substrate
GKGAN 42:46 (≠ GKGSN 40:44) binding substrate
E143 (= E141) binding substrate
N187 (= N185) binding ATP
TLGSRG 223:228 (≠ TLGENG 221:226) binding ATP
GD 254:255 (= GD 253:254) binding ATP
D255 (= D254) binding substrate
H279 (≠ T278) binding ATP

1gqtB Activation of ribokinase by monovalent cations (see paper)
39% identity, 98% coverage: 5:304/306 of query aligns to 6:304/307 of 1gqtB

query
sites
1gqtB

I

L

V

V

I

V

L

L

G

G

V

S

F

I

V
x
N

A

A

D
|
D

T

H

A

I

Y

L

R

N

A

L

Q

Q

R

S

Q

F

P

P

R

T

L

P

G

G

E

E

T

T

I

V

L

T

G

G

Q

N

S

H

F

Y

A

Q

L

V

G

A

P

F

G

G

G
|
G

K

K

G

G

S

A

N
|
N

Q

Q

S

A

V

V

A

A

A

A

G

G

M

R

A

S

G

G

A

A

S

N

V

I

G

A

F

F

I

I

T

A

K

C

L

T

G

G

K

D

D

D

P

S

F

I

A

G

D

E

-

S

-

V

-

R

M

Q

A

Q

L

L

A

A

T

T

W

-

K

D

K

N

A

I

G

D

V

I

T

T

S

P

L

V

A

S

T

V

R

I

H

K

A

G

D

E

D

S

Y

-

T

T

G

G

A

V

A

A

Y

L

I

I

F

F

V

V

D

N

E

-

N

G

T

E

G

G

D

E

N

N

A

V

I

I

I

G

V

I

C

H

P

A

G

G

A

A

A

N

A

A

T

A

I

L

N

S

R

P

S

A

D

L

I

V

D

E

S

A

H

Q

A

R

E

E

V

R

I

I

K

A

Q

N

C

A

K

S

V

A

L

L

M

L

T

M

Q

Q

L

L

E
|
E

Q

S

P

P

I

L

D

E

A

S

A

V

E

M

Y

A

A

A

L

A

A

K

C

I

A

A

R

H

K

Q

A

N

G

K

A

T

T

I

T

V

I

A

L

L

N

N

P

P

A

A

P

P

A

A

V

R

E

E

L

L

P

P

T

D

S

E

M

L

I

L

A

A

L

L

C

V

D

D

F

I

V

I

T

T

P

P

N
|
N

E

E

I

T

E

E

A

A

E

E

T

K

I

L

T

T

G

G

I

I

T

R

V

V

N

E

S

N

P

D

K

E

D

D

A

A

V

A

A

K

A

A

A

A

E

Q

A

V

L

L

L

H

A

E

K

K

G

G

A

I

G

R

G

T

A

V

I

L

I

I

T
|
T

L

L

G
|
G

E
x
S

N

R

G

G

C

V

L

W

Y

A

H

S

D

V

G

N

S

G

N

E

S

G

V

Q

H

R

V

V

P

P

A

G

I

F

N

R

A

V

G

-

P

Q

V

A

V

V

E
x
D

T

T

T
x
I

G
x
A

A
|
A

G
|
G

D
|
D

A

T

F

F

N

N

G

G

G

A

F

L

A

I

V

T

A

A

I

L

A

L

E

E

G

E

H

K

S

P

P

L

L

P

E

E

A

A

I

I

R

R

F

F

G

A

T
x
H

A

A

T

A

A
|
A

A

A

I

I

S
x
A

V

V

T

T

R
|
R

P

K

G

G

T

A

A

Q

P

P

S
|
S

M

V

P

P

T

W

R

R

K

E

E

E

I

I

E

D

S

A

L

F

L

L

active site: A251 (≠ G251), A252 (= A252), G253 (= G253), D254 (= D254)
binding phosphomethylphosphonic acid adenylate ester: N186 (= N185), T222 (= T221), G224 (= G223), S225 (≠ E224), A252 (= A252), G253 (= G253), H278 (≠ T278), A281 (= A281)
binding cesium ion: D248 (≠ E248), I250 (≠ T250), A284 (≠ S284), R287 (= R287), S293 (= S293)
binding alpha-D-ribofuranose: N13 (≠ V12), D15 (= D14), G41 (= G40), N45 (= N44), E142 (= E141), D254 (= D254)

2fv7A Crystal structure of human ribokinase
37% identity, 97% coverage: 5:300/306 of query aligns to 4:303/308 of 2fv7A

query
sites
2fv7A

I

V

V

V

I

V

L

V

G

G

V

S

F

C

V

M

A

T

D

D

T

L

A

V

Y

S

R

L

A

T

Q

S

R

R

Q

L

P

P

R

K

L

T

G

G

E

E

T

T

I

I

L

H

G

G

Q

H

S

K

F

F

A

F

L

I

G

G

P

F

G

G

G

G

K

K

G

G

S

A

N

N

Q

Q

S

C

V

V

A

Q

A

A

G

A

M

R

A

L

G

G

A

A

S

M

V

T

G

S

F

M

I

V

T

C

K

K

L

V

G

G

K

K

D

D

P

S

F

F

A

G

D

N

M

D

A

Y

L

I

A

E

T

N

W

L

K

K

K

Q

A

N

G

D

V

I

T

S

S

T

L

E

A

F

T

T

R

Y

H

Q

A

T

D

K

D

D

-

A

Y

A

T

T

G

G

A

T

A

A

Y

S

I

I

F

I

V

V

D

N

E

-

N

N

T

E

G

G

D

Q

N

N

A

I

I

I

I

V

V

I

C

V

P

A

G

G

A

A

A

N

A

L

T

L

I

L

N

N

R

T

S

E

D

D

I

L

D

R

S

A

H

A

A

A

E

N

V

V

I

I

K

S

Q

R

C

A

K

K

V

V

L

M

M

V

T

C

Q

Q

L

L

E

E

Q

I

P

T

I

P

D

A

A

T

A

S

E

L

Y

E

A

A

L

L

A

T

C

M

A

A

R

R

K

R

A

S

G

G

A

V

T

K

T

T

I

L

L

F

N

N

P

P

A

A

P

P

A

A

V

I

-

A

E

D

L

L

P

D

T

P

S

Q

M

F

I

Y

A

T

L

L

C

S

D

D

F

V

V

F

T

C

P

C

N
|
N

E

E

I

S

E

E

A

A

E

E

T

I

I

L

T

T

G

G

I

L

T

T

V

V

N

G

S

S

P

A

K

A

D

D

A

A

V

G

A

E

A

A

A

A

E

L

A

V

L

L

L

L

A

K

K

R

G

G

A

C

G

Q

G

V

A

V

I

I

I

I

T
|
T

L

L

G
|
G

E

A

N

E

G
|
G

C

C

-

V

-

V

L

L

Y

S

H

Q

D

T

G

E

S

P

N

E

S

P

V

K

H

H

V

I

P

P

A
x
T

I

E

N

K

A
x
V

G

-

P

K

V

A

V

V

E

D

T

T

T

T

G
|
G

A
|
A

G
|
G

D
|
D

A

S

F

F

N

V

G

G

G

A

F

L

A

A

V

F

A

Y

I

L

A

A

E

Y

-

Y

-

P

G

N

H

L

S

S

P

L

L

E

E

D

A

M

I

L

R

N

F

R

G

S

T
x
N

A

F

T

I

A
|
A

A
|
A

I

V

S

S

V
|
V

T

Q

R

A

P

A

G

G

T

T

A

Q

P

S

S

S

M

Y

P

P

T

Y

R

K

K

K

E

D

I

L

active site: G252 (= G251), A253 (= A252), G254 (= G253), D255 (= D254)
binding adenosine-5'-diphosphate: N185 (= N185), T221 (= T221), G223 (= G223), G226 (= G226), T242 (≠ A240), V245 (≠ A243), A253 (= A252), G254 (= G253), N281 (≠ T278), A284 (= A281), A285 (= A282), V288 (= V285)

6wjzA Crystal structure of human ribokinase in complex with ampcp
37% identity, 97% coverage: 5:300/306 of query aligns to 5:304/315 of 6wjzA

query
sites
6wjzA

I

V

V

V

I

V

L

V

G

G

V

S

F

C

V

M

A

T

D

D

T

L

A

V

Y

S

R

L

A

T

Q

S

R

R

Q

L

P

P

R

K

L

T

G

G

E

E

T

T

I

I

L

H

G

G

Q

H

S

K

F

F

A

F

L

I

G

G

P

F

G

G

G

G

K

K

G

G

S

A

N

N

Q

Q

S

C

V

V

A

Q

A

A

G

A

M

R

A

L

G

G

A

A

S

M

V

T

G

S

F

M

I

V

T

C

K

K

L

V

G

G

K

K

D

D

P

S

F

F

A

G

D

N

M

D

A

Y

L

I

A

E

T

N

W

L

K

K

K

Q

A

N

G

D

V

I

T

S

S

T

L

E

A

F

T

T

R

Y

H

Q

A

T

D

K

D

D

-

A

Y

A

T

T

G

G

A

T

A

A

Y

S

I

I

F

I

V

V

D

N

E

-

N

N

T

E

G

G

D

Q

N

N

A

I

I

I

I

V

V

I

C

V

P

A

G

G

A

A

A

N

A

L

T

L

I

L

N

N

R

T

S

E

D

D

I

L

D

R

S

A

H

A

A

A

E

N

V

V

I

I

K

S

Q

R

C

A

K

K

V

V

L

M

M

V

T

C

Q

Q

L

L

E

E

Q

I

P

T

I

P

D

A

A

T

A

S

E

L

Y

E

A

A

L

L

A

T

C

M

A

A

R

R

K

R

A

S

G

G

A

V

T

K

T

T

I

L

L

F

N

N

P

P

A

A

P

P

A

A

V

I

-

A

E

D

L

L

P

D

T

P

S

Q

M

F

I

Y

A

T

L

L

C

S

D

D

F

V

V

F

T

C

P

C

N
|
N

E

E

I

S

E

E

A

A

E

E

T

I

I

L

T

T

G

G

I

L

T

T

V

V

N

G

S

S

P

A

K

A

D

D

A

A

V

G

A

E

A

A

A

A

E

L

A

V

L

L

L

L

A

K

K

R

G

G

A

C

G

Q

G

V

A

V

I

I

I

I

T
|
T

L

L

G
|
G

E
x
A

N

E

G
|
G

C

C

-

V

-

V

L

L

Y

S

H

Q

D

T

G

E

S

P

N

E

S

P

V

K

H

H

V

I

P

P

A
x
T

I

E

N

K

A
x
V

G

-

P

K

V

A

V

V

E

D

T

T

T

T

G

G

A
|
A

G
|
G

D

D

A

S

F

F

N

V

G

G

G

A

F

L

A

A

V

F

A

Y

I

L

A

A

E

Y

-

Y

-

P

G

N

H

L

S

S

P

L

L

E

E

D

A

M

I

L

R

N

F

R

G

S

T
x
N

A

F

T

I

A
|
A

A
|
A

I

V

S

S

V
|
V

T

Q

R

A

P

A

G

G

T

T

A

Q

P

S

S

S

M

Y

P

P

T

Y

R

K

K

K

E

D

I

L

binding phosphomethylphosphonic acid adenosyl ester: N186 (= N185), T222 (= T221), G224 (= G223), A225 (≠ E224), G227 (= G226), T243 (≠ A240), V246 (≠ A243), A254 (= A252), G255 (= G253), N282 (≠ T278), A285 (= A281), A286 (= A282), V289 (= V285)

6wk0B Crystal structure of human ribokinase in complex with amppcp and ribose
37% identity, 97% coverage: 5:300/306 of query aligns to 5:304/311 of 6wk0B

query
sites
6wk0B

I

V

V

V

I

V

L

V

G

G

V

S

F

C

V

M

A

T

D
|
D

T

L

A

V

Y

S

R

L

A

T

Q

S

R

R

Q

L

P

P

R

K

L

T

G

G

E

E

T

T

I

I

L

H

G

G

Q

H

S

K

F

F

A

F

L

I

G

G

P

F

G

G

G
|
G

K
|
K

G

G

S

A

N
|
N

Q

Q

S

C

V

V

A

Q

A

A

G

A

M

R

A

L

G

G

A

A

S

M

V

T

G

S

F

M

I

V

T

C

K

K

L

V

G

G

K

K

D

D

P

S

F

F

A

G

D

N

M

D

A

Y

L

I

A

E

T

N

W

L

K

K

K

Q

A

N

G

D

V

I

T

S

S

T

L

E

A

F

T

T

R

Y

H

Q

A

T

D

K

D

D

-

A

Y

A

T

T

G

G

A

T

A
|
A

Y

S

I

I

F

I

V

V

D

N

E

-

N

N

T

E

G

G

D

Q

N

N

A

I

I

I

I

V

V

I

C

V

P

A

G

G

A

A

A

N

A

L

T

L

I

L

N

N

R

T

S

E

D

D

I

L

D

R

S

A

H

A

A

A

E

N

V

V

I

I

K

S

Q

R

C

A

K

K

V

V

L

M

M

V

T

C

Q

Q

L

L

E
|
E

Q

I

P

T

I

P

D

A

A

T

A

S

E

L

Y

E

A

A

L

L

A

T

C

M

A

A

R

R

K

R

A

S

G

G

A

V

T

K

T

T

I

L

L

F

N

N

P

P

A

A

P

P

A

A

V

I

-

A

E

D

L

L

P

D

T

P

S

Q

M

F

I

Y

A

T

L

L

C

S

D

D

F

V

V

F

T

C

P

C

N
|
N

E

E

I

S

E

E

A

A

E

E

T

I

I

L

T

T

G

G

I

L

T

T

V

V

N

G

S

S

P

A

K

A

D

D

A

A

V

G

A

E

A

A

A

A

E

L

A

V

L

L

L

L

A

K

K

R

G

G

A

C

G

Q

G

V

A

V

I

I

I

I

T
|
T

L

L

G
|
G

E
x
A

N

E

G
|
G

C

C

-

V

-

V

L

L

Y

S

H

Q

D

T

G

E

S

P

N

E

S

P

V

K

H

H

V

I

P

P

A
x
T

I

E

N

K

A
x
V

G

-

P

K

V

A

V

V

E

D

T

T

T

T

G

G

A
|
A

G
|
G

D
|
D

A

S

F

F

N

V

G

G

G

A

F

L

A

A

V

F

A

Y

I

L

A

A

E

Y

-

Y

-

P

G

N

H

L

S

S

P

L

L

E

E

D

A

M

I

L

R

N

F

R

G

S

T
x
N

A

F

T

I

A
|
A

A
|
A

I

V

S

S

V
|
V

T

Q

R

A

P

A

G

G

T

T

A

Q

P

S

S

S

M

Y

P

P

T

Y

R

K

K

K

E

D

I

L

binding phosphomethylphosphonic acid adenylate ester: N186 (= N185), T222 (= T221), G224 (= G223), A225 (≠ E224), G227 (= G226), T243 (≠ A240), V246 (≠ A243), A254 (= A252), G255 (= G253), N282 (≠ T278), A285 (= A281), A286 (= A282), V289 (= V285)
binding alpha-D-ribofuranose: D14 (= D14), G40 (= G40), K41 (= K41), N44 (= N44), A96 (= A95), E141 (= E141), D256 (= D254)

5c41A Crystal structure of human ribokinase in complex with amppcp in p21 spacegroup and with 4 protomers
37% identity, 97% coverage: 5:300/306 of query aligns to 5:304/317 of 5c41A

query
sites
5c41A

I

V

V

V

I

V

L

V

G

G

V

S

F

C

V

M

A

T

D

D

T

L

A

V

Y

S

R

L

A

T

Q

S

R

R

Q

L

P

P

R

K

L

T

G

G

E

E

T

T

I

I

L

H

G

G

Q

H

S

K

F

F

A

F

L

I

G

G

P

F

G

G

G

G

K

K

G

G

S

A

N

N

Q

Q

S

C

V

V

A

Q

A

A

G

A

M

R

A

L

G

G

A

A

S

M

V

T

G

S

F

M

I

V

T

C

K

K

L

V

G

G

K

K

D

D

P

S

F

F

A

G

D

N

M

D

A

Y

L

I

A

E

T

N

W

L

K

K

K

Q

A

N

G

D

V

I

T

S

S

T

L

E

A

F

T

T

R

Y

H

Q

A

T

D

K

D

D

-

A

Y

A

T

T

G

G

A

T

A

A

Y

S

I

I

F

I

V

V

D

N

E

-

N

N

T

E

G

G

D

Q

N

N

A

I

I

I

I

V

V

I

C

V

P

A

G

G

A

A

A

N

A

L

T

L

I

L

N

N

R

T

S

E

D

D

I

L

D

R

S

A

H

A

A

A

E

N

V

V

I

I

K

S

Q

R

C

A

K

K

V

V

L

M

M

V

T

C

Q

Q

L

L

E

E

Q

I

P

T

I

P

D

A

A

T

A

S

E

L

Y

E

A

A

L

L

A

T

C

M

A

A

R

R

K

R

A

S

G

G

A

V

T

K

T

T

I

L

L

F

N

N

P

P

A

A

P

P

A

A

V

I

-

A

E

D

L

L

P

D

T

P

S

Q

M

F

I

Y

A

T

L

L

C

S

D

D

F

V

V

F

T

C

P

C

N
|
N

E

E

I

S

E

E

A

A

E

E

T

I

I

L

T

T

G

G

I

L

T

T

V

V

N

G

S

S

P

A

K

A

D

D

A

A

V

G

A

E

A

A

A

A

E

L

A

V

L

L

L

L

A

K

K

R

G

G

A

C

G

Q

G

V

A

V

I

I

I

I

T
|
T

L

L

G
|
G

E
x
A

N

E

G
|
G

C

C

-

V

-

V

L

L

Y

S

H

Q

D

T

G

E

S

P

N

E

S

P

V

K

H

H

V

I

P

P

A

T

I

E

N

K

A
x
V

G

-

P

K

V

A

V

V

E

D

T

T

T

T

G
|
G

A
|
A

G
|
G

D
|
D

A

S

F

F

N

V

G

G

G

A

F

L

A

A

V

F

A

Y

I

L

A

A

E

Y

-

Y

-

P

G

N

H

L

S

S

P

L

L

E

E

D

A

M

I

L

R

N

F

R

G

S

T
x
N

A

F

T

I

A
|
A

A
|
A

I

V

S

S

V

V

T

Q

R

A

P

A

G

G

T

T

A

Q

P

S

S

S

M

Y

P

P

T

Y

R

K

K

K

E

D

I

L

active site: G253 (= G251), A254 (= A252), G255 (= G253), D256 (= D254)
binding phosphomethylphosphonic acid adenylate ester: N186 (= N185), T222 (= T221), G224 (= G223), A225 (≠ E224), G227 (= G226), V246 (≠ A243), G255 (= G253), N282 (≠ T278), A285 (= A281), A286 (= A282)

5c3yA Structure of human ribokinase crystallized with amppnp
37% identity, 97% coverage: 5:300/306 of query aligns to 4:303/306 of 5c3yA

query
sites
5c3yA

I

V

V

V

I

V

L

V

G

G

V

S

F

C

V

M

A

T

D

D

T

L

A

V

Y

S

R

L

A

T

Q

S

R

R

Q

L

P

P

R

K

L

T

G

G

E

E

T

T

I

I

L

H

G

G

Q

H

S

K

F

F

A

F

L

I

G

G

P

F

G

G

G

G

K

K

G

G

S

A

N

N

Q

Q

S

C

V

V

A

Q

A

A

G

A

M

R

A

L

G

G

A

A

S

M

V

T

G

S

F

M

I

V

T

C

K

K

L

V

G

G

K

K

D

D

P

S

F

F

A

G

D

N

M

D

A

Y

L

I

A

E

T

N

W

L

K

K

K

Q

A

N

G

D

V

I

T

S

S

T

L

E

A

F

T

T

R

Y

H

Q

A

T

D

K

D

D

-

A

Y

A

T

T

G

G

A

T

A

A

Y

S

I

I

F

I

V

V

D

N

E

-

N

N

T

E

G

G

D

Q

N

N

A

I

I

I

I

V

V

I

C

V

P

A

G

G

A

A

A

N

A

L

T

L

I

L

N

N

R

T

S

E

D

D

I

L

D

R

S

A

H

A

A

A

E

N

V

V

I

I

K

S

Q

R

C

A

K

K

V

V

L

M

M

V

T

C

Q

Q

L

L

E

E

Q

I

P

T

I

P

D

A

A

T

A

S

E

L

Y

E

A

A

L

L

A

T

C

M

A

A

R

R

K

R

A

S

G

G

A

V

T

K

T

T

I

L

L

F

N

N

P

P

A

A

P

P

A

A

V

I

-

A

E

D

L

L

P

D

T

P

S

Q

M

F

I

Y

A

T

L

L

C

S

D

D

F

V

V

F

T

C

P

C

N

N

E

E

I

S

E

E

A

A

E

E

T

I

I

L

T

T

G

G

I

L

T

T

V

V

N

G

S

S

P

A

K

A

D

D

A

A

V

G

A

E

A

A

A

A

E

L

A

V

L

L

L

L

A

K

K

R

G

G

A

C

G

Q

G

V

A

V

I

I

I

I

T
|
T

L

L

G
|
G

E

A

N

E

G

G

C

C

-

V

-

V

L

L

Y

S

H

Q

D

T

G

E

S

P

N

E

S

P

V

K

H

H

V

I

P

P

A

T

I

E

N

K

A
x
V

G

-

P

K

V

A

V

V

E

D

T
|
T

T

T

G
|
G

A
|
A

G
|
G

D
|
D

A

S

F

F

N

V

G

G

G

A

F

L

A

A

V

F

A

Y

I

L

A

A

E

Y

-

Y

-

P

G

N

H

L

S

S

P

L

L

E

E

D

A

M

I

L

R

N

F

R

G

S

T
x
N

A

F

T

I

A
|
A

A
|
A

I

V

S

S

V

V

T

Q

R

A

P

A

G

G

T

T

A

Q

P

S

S

S

M

Y

P

P

T

Y

R

K

K

K

E

D

I

L

active site: G252 (= G251), A253 (= A252), G254 (= G253), D255 (= D254)
binding amp phosphoramidate: T221 (= T221), G223 (= G223), V245 (≠ A243), T250 (= T249), G254 (= G253), N281 (≠ T278), A284 (= A281), A285 (= A282)

5byfA Crystal structure of human ribokinase in complex with amp
38% identity, 95% coverage: 9:300/306 of query aligns to 10:305/313 of 5byfA

query
sites
5byfA

G

G

V

S

F

C

V

M

A

T

D

D

T

L

A

V

Y

S

R

L

A

T

Q

S

R

R

Q

L

P

P

R

K

L

T

G

G

E

E

T

T

I

I

L

H

G

G

Q

H

S

K

F

F

A

F

L

I

G

G

P

F

G

G

G

G

K

K

G

G

S

A

N

N

Q

Q

S

C

V

V

A

Q

A

A

G

A

M

R

A

L

G

G

A

A

S

M

V

T

G

S

F

M

I

V

T

C

K

K

L

V

G

G

K

K

D

D

P

S

F

F

A

G

D

N

M

D

A

Y

L

I

A

E

T

N

W

L

K

K

K

Q

A

N

G

D

V

I

T

S

S

T

L

E

A

F

T

T

R

Y

H

Q

A

T

D

K

D

D

-

A

Y

A

T

T

G

G

A

T

A

A

Y

S

I

I

F

I

V

V

D

N

E

-

N

N

T

E

G

G

D

Q

N

N

A

I

I

I

I

V

V

I

C

V

P

A

G

G

A

A

A

N

A

L

T

L

I

L

N

N

R

T

S

E

D

D

I

L

D

R

S

A

H

A

A

A

E

N

V

V

I

I

K

S

Q

R

C

A

K

K

V

V

L

M

M

V

T

C

Q

Q

L

L

E

E

Q

I

P

T

I

P

D

A

A

T

A

S

E

L

Y

E

A

A

L

L

A

T

C

M

A

A

R

R

K

R

A

S

G

G

A

V

T

K

T

T

I

L

L

F

N

N

P

P

A

A

P

P

A

A

V

I

-

A

E

D

L

L

P

D

T

P

S

Q

M

F

I

Y

A

T

L

L

C

S

D

D

F

V

V

F

T

C

P

C

N

N

E

E

I

S

E

E

A

A

E

E

T

I

I

L

T

T

G

G

I

L

T

T

V

V

N

G

S

S

P

A

K

A

D

D

A

A

V

G

A

E

A

A

A

A

E

L

A

V

L

L

L

L

A

K

K

R

G

G

A

C

G

Q

G

V

A

V

I

I

I

I

T
|
T

L

L

G
|
G

E
x
A

N

E

G
|
G

C

C

-

V

-

V

L

L

Y

S

H

Q

D

T

G

E

S

P

N

E

S

P

V

K

H

H

V

I

P

P

A

T

I

E

N

K

A

V

G

-

P

K

V

A

V

V

E

D

T

T

T

T

G
|
G

A
|
A

G
|
G

D
|
D

A

S

F

F

N

V

G

G

G

A

F

L

A

A

V

F

A

Y

I

L

A

A

E

Y

-

Y

-

P

G

N

H

L

S

S

P

L

L

E

E

D

A

M

I

L

R

N

F

R

G

S

T
x
N

A

F

T

I

A
|
A

A

A

I

V

S

S

V

V

T

Q

R

A

P

A

G

G

T

T

A

Q

P

S

S

S

M

Y

P

P

T

Y

R

K

K

K

E

D

I

L

active site: G254 (= G251), A255 (= A252), G256 (= G253), D257 (= D254)
binding adenosine monophosphate: T223 (= T221), G225 (= G223), A226 (≠ E224), G228 (= G226), N283 (≠ T278), A286 (= A281)

Q9H477 Ribokinase; RK; EC 2.7.1.15 from Homo sapiens (Human)
38% identity, 95% coverage: 9:300/306 of query aligns to 22:317/322 of Q9H477

query
sites
Q9H477

G

G

V

S

F

C

V

M

A

T

D

D

T

L

A

V

Y

S

R

L

A

T

Q

S

R

R

Q

L

P

P

R

K

L

T

G

G

E

E

T

T

I

I

L

H

G

G

Q

H

S

K

F

F

A

F

L

I

G

G

P

F

G

G

G

G

K

K

G

G

S

A

N

N

Q

Q

S

C

V

V

A

Q

A

A

G

A

M

R

A

L

G

G

A

A

S

M

V

T

G

S

F

M

I

V

T

C

K

K

L

V

G

G

K

K

D

D

P

S

F

F

A

G

D

N

M

D

A

Y

L

I

A

E

T

N

W

L

K

K

K

Q

A

N

G

D

V

I

T

S

S

T

L

E

A

F

T

T

R

Y

H

Q

A

T

D

K

D

D

-

A

Y

A

T

T

G

G

A

T

A

A

Y

S

I

I

F

I

V

V

D

N

E

-

N

N

T

E

G

G

D

Q

N

N

A

I

I

I

I

V

V

I

C

V

P

A

G

G

A

A

A

N

A

L

T

L

I

L

N

N

R

T

S

E

D

D

I

L

D

R

S

A

H

A

A

A

E

N

V

V

I

I

K

S

Q

R

C

A

K

K

V

V

L

M

M

V

T

C

Q

Q

L

L

E

E

Q

I

P

T

I

P

D

A

A

T

A

S

E

L

Y

E

A

A

L

L

A

T

C

M

A

A

R

R

K

R

A

S

G

G

A

V

T

K

T

T

I

L

L

F

N

N

P

P

A

A

P

P

A

A

V

I

-

A

E

D

L

L

P

D

T

P

S

Q

M

F

I

Y

A

T

L

L

C

S

D

D

F

V

V

F

T

C

P

C

N
|
N

E

E

I

S

E

E

A

A

E

E

T

I

I

L

T

T

G

G

I

L

T

T

V

V

N

G

S

S

P

A

K

A

D

D

A

A

V

G

A

E

A

A

A

A

E

L

A

V

L

L

L

L

A

K

K

R

G

G

A

C

G

Q

G

V

A

V

I

I

I

I

T
|
T

L
|
L

G
|
G

E
x
A

N
x
E

G
|
G

C

C

-

V

-

V

L

L

Y

S

H

Q

D

T

G

E

S

P

N

E

S

P

V

K

H

H

V

I

P

P

A
x
T

I

E

N

K

A

V

G

-

P

K

V

A

V

V

E

D

T

T

T

T

G

G

A

A

G
|
G

D
|
D

A

S

F

F

N

V

G

G

-

A

-

L

G

A

F

F

A

Y

V

L

A

A

I

Y

A

Y

E

P

G

N

H

L

S

S

P

L

L

E

E

D

A

M

I

L

R

N

F

R

G

S

T
x
N

A

F

T

I

A

A

A

A

I

V

S

S

V

V

T

Q

R

A

P

A

G

G

T

T

A

Q

P

S

S

S

M

Y

P

P

T

Y

R

K

K

K

E

D

I

L

N199 (= N185) binding ATP
TLGAEG 235:240 (≠ TLGENG 221:226) binding ATP
T256 (≠ A240) binding ATP
GD 268:269 (= GD 253:254) binding ATP
N295 (≠ T278) binding ATP

6a8cA Ribokinase from leishmania donovani with adp (see paper)
34% identity, 97% coverage: 4:300/306 of query aligns to 15:322/327 of 6a8cA

query
sites
6a8cA

D

D

I

I

V

L

I

V

L

V

G

G

V

S

F

C

V

F

A

L

D
|
D

T

Y

A

V

Y

G

R

Y

A

V

Q

D

R

H

Q

M

P

P

R

Q

L

V

G

G

E

E

T

T

I

M

L

H

G
x
S

Q

V

S
|
S

F

F

A

H

L

K

G

G

P

F

G
|
G

G
|
G

K

K

G

G

S

A

N
|
N

Q

Q

S

A

V

V

A

A

A

A

G

G

M

R

A

L

G

G

A

A

S

K

V

V

G

A

F

M

I

V

T

S

K

M

L

V

G

G

K

T

D

D

P

G

F

D

A

G

D

S

M

D

A

Y

L

I

A

K

T

E

W

L

K

E

K

R

A

N

G

G

V

V

-

D

T

T

S

A

L

Y

A

M

T

F

R

R

H

T

A

G

D

K

D

S

Y

S

T

T

G

G

A

L

A

A

Y

M

I

I

F

L

V

V

D

D

E

T

N

K

T

S

G

S

D

N

N

N

A

E

I

I

I

V

V

I

C

C

P

P

G

N

A

A

A

T

A

N

T

H

I

F

N

T

-

P

-

E

-

L

-

L

R

R

S

A

D

Q

I

T

D

N

S

N

H

Y

A

E

E

R

V

I

I

L

K

H

Q

T

-

G

C

L

K

K

V

Y

L

L

M

I

T

C

Q

Q

L

N

E

E

Q

I

P

P

I

L

D

P

A

T

A

T

E

L

Y

D

A

T

L

I

A

K

C

E

A

A

R

H

K

S

A

R

G

G

A

V

T

Y

T

T

I

V

L

F

N

N

P

S

A

A

P

P

A

A

-

P

-

K

-

P

-

A

-

E

V

V

E

E

L

Q

P

I

T

K

S

P

M

F

I

L

A

P

L

Y

C

V

D

S

F

L

V

F

T

C

P

P

N

N

E

E

I

V

E

E

A

A

E

T

T

L

I

I

T

T

G

G

I

V

T

K

V

V

N

T

S

D

P

T

K

E

D

S

A

A

V

F

A

S

A

A

A

I

E

K

A

A

L

L

L

Q

A

Q

K

L

G

G

A

V

G

R

G

D

A

V

I

V

I

I

T

T

L

L

G
|
G

E
x
A

N

A

G

G

-

F

C

V

L

L

Y

S

H

E

D

N

G

G

S

A

N

E

S

P

V

V

H

H

V

V

P

T

A

G

I

K

N

H

A

V

G

-

P

K

V

A

V

V

E

D

T
|
T

T

T

G

G

A
|
A

G
|
G

D

D

A

C

F

F

N

V

G

G

G

S

F

M

A

V

V

Y

A

F

I

M

A

S

E

R

G

G

H

R

S

N

P

L

L

L

E

E

A

A

I

C

R

K

F

R

G

A

T
x
N

A

E

T

C

A
|
A

A

A

I

I

S

S

V

V

T

T

R

R

P

K

G

G

T

T

A

Q

P

L

S

S

M

Y

P

P

T

H

R

P

K

S

E

E

I

L

binding adenosine-5'-diphosphate: G245 (= G223), A246 (≠ E224), T271 (= T249), A274 (= A252), G275 (= G253), N300 (≠ T278), A303 (= A281)
binding glycerol: D25 (= D14), S42 (≠ G31), S44 (= S33), G50 (= G39), G51 (= G40), N55 (= N44)

6a8bA Ribokinase from leishmania donovani with amppcp (see paper)
34% identity, 97% coverage: 4:300/306 of query aligns to 15:322/327 of 6a8bA

query
sites
6a8bA

D

D

I

I

V

L

I

V

L

V

G

G

V

S

F

C

V

F

A

L

D
|
D

T

Y

A

V

Y

G

R

Y

A

V

Q

D

R

H

Q

M

P

P

R

Q

L

V

G

G

E

E

T

T

I

M

L

H

G

S

Q

V

S

S

F

F

A

H

L

K

G

G

P

F

G
|
G

G
|
G

K

K

G

G

S

A

N
|
N

Q

Q

S

A

V

V

A

A

A

A

G

G

M

R

A

L

G

G

A

A

S

K

V

V

G

A

F

M

I

V

T

S

K

M

L

V

G

G

K

T

D

D

P

G

F

D

A

G

D

S

M

D

A

Y

L

I

A

K

T

E

W

L

K

E

K

R

A

N

G

G

V

V

-

D

T

T

S

A

L

Y

A

M

T

F

R

R

H

T

A

G

D

K

D

S

Y

S

T

T

G

G

A

L

A

A

Y

M

I

I

F

L

V

V

D

D

E

T

N

K

T

S

G

S

D

N

N

N

A

E

I

I

I

V

V

I

C

C

P

P

G

N

A

A

A

T

A

N

T

H

I

F

N

T

-

P

-

E

-

L

-

L

R</