SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_044138357.1 NCBI__GCF_000220155.1:WP_044138357.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P09114 Acetolactate synthase 2, chloroplastic; ALS II; Acetohydroxy-acid synthase II; Acetolactate synthase II; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see paper)
45% identity, 98% coverage: 13:569/569 of query aligns to 93:657/664 of P09114

query
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P09114

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P191 (≠ V112) mutation to A: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with L-568.
W568 (= W488) mutation to L: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with A-191.

P09342 Acetolactate synthase 1, chloroplastic; ALS I; Acetohydroxy-acid synthase I; Acetolactate synthase I; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see 2 papers)
45% identity, 98% coverage: 13:569/569 of query aligns to 96:660/667 of P09342

query
sites
P09342

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C161 (= C79) modified: Disulfide link with 307
P194 (≠ V112) mutation to Q: In C3; highly resistant to sulfonylurea herbicides.
C307 (≠ V223) modified: Disulfide link with 161

7tzzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase p197t mutant in complex with bispyribac-sodium (see paper)
44% identity, 98% coverage: 13:569/569 of query aligns to 14:578/582 of 7tzzA

query
sites
7tzzA

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binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: M266 (= M266), R292 (= R292), W489 (= W488), S568 (≠ A559)
binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V399), G401 (= G400), Q402 (= Q401), H403 (= H402), G426 (= G425), M428 (= M427), G452 (= G451), D453 (= D452), G454 (= G453), S455 (≠ G454), L483 (= L482), G484 (= G483), M485 (= M484), V486 (= V485)
binding flavin-adenine dinucleotide: R161 (= R161), G222 (= G220), G223 (≠ Q221), G224 (= G222), T246 (= T246), L247 (= L247), M248 (≠ L248), M263 (= M263), L264 (= L264), M266 (= M266), H267 (= H267), G286 (= G286), R288 (= R288), V293 (= V293), D310 (≠ E310), I311 (= I311), D329 (≠ N329), V330 (= V330), M405 (= M404), G423 (= G422)
binding magnesium ion: A37 (= A36), T82 (= T82), S83 (= S83), Q122 (= Q122), Y381 (vs. gap), D453 (= D452), M458 (= M457), Q461 (= Q460), N480 (= N479), H482 (≠ F481), K533 (≠ R524)

P17597 Acetolactate synthase, chloroplastic; AtALS; Acetohydroxy-acid synthase; Protein CHLORSULFURON RESISTANT 1; EC 2.2.1.6 from Arabidopsis thaliana (Mouse-ear cress) (see 8 papers)
44% identity, 98% coverage: 13:569/569 of query aligns to 99:663/670 of P17597

query
sites
P17597

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A122 (= A36) mutation to V: Reduced catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
M124 (= M38) mutation to E: Reduced catalytic activity. Resistant to imidazolinone herbicides and reduced sensitivity to sulfonylurea herbicides.; mutation to I: No effect on catalytic activity. Increased resistance to imidazolinone herbicides.
E144 (= E59) binding thiamine diphosphate
S186 (= S101) binding FAD
P197 (≠ V112) mutation to S: In csr1-1/GH50; resistant to sulfonylurea but not to imidazolinone herbicides.
R199 (≠ S114) mutation R->A,E: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
Q207 (= Q122) binding thiamine diphosphate
K220 (= K135) binding (R)-imazaquin
R246 (= R161) binding (R)-imazaquin; binding FAD
K256 (= K171) binding chlorimuron-ethyl
G308 (≠ Q221) binding FAD
TL 331:332 (= TL 246:247) binding FAD
C340 (≠ S255) modified: Cysteine sulfinic acid (-SO2H)
LGMH 349:352 (= LGMH 264:267) binding FAD
GVRFD 371:375 (≠ GMRFD 286:290) binding FAD
DR 376:377 (= DR 291:292) binding chlorimuron-ethyl
DI 395:396 (≠ EI 310:311) binding FAD
DV 414:415 (≠ NV 329:330) binding FAD
QH 487:488 (= QH 401:402) binding thiamine diphosphate
GG 508:509 (= GG 422:423) binding FAD
GAM 511:513 (≠ GTM 425:427) binding thiamine diphosphate
D538 (= D452) binding Mg(2+)
DGS 538:540 (≠ DGG 452:454) binding thiamine diphosphate
N565 (= N479) binding Mg(2+)
NQHLGM 565:570 (≠ NNFLGM 479:484) binding thiamine diphosphate
H567 (≠ F481) binding Mg(2+)
W574 (= W488) binding chlorimuron-ethyl; mutation to L: Increased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.; mutation to S: Slightly decreased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.
S653 (≠ A559) binding chlorimuron-ethyl; mutation to A: No effect on catalytic activity or sensitivity to herbicides.; mutation to F: No effect on catalytic activity. Resistant to imidazolinone herbicides and also slightly sulfonylurea-resistant.; mutation to N: In csr1-2/GH90; no effect on catalytic activity. Resistant to imidazolinone but not to sulfonylurea herbicides.; mutation to T: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.

3ea4A Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron-ester (see paper)
44% identity, 98% coverage: 13:569/569 of query aligns to 13:577/582 of 3ea4A

query
sites
3ea4A

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I

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E

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L

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V

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-

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I

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D

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K

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R

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L

-

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-

D

-

P

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A

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-

E

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I

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P

P

D

N

F

M

Q

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T

L

I

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A

A

K

A

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A

Y

C

H

G

I

I

P

P

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A

L

A

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V

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K

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K

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A

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D

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L

D

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E

A

A

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M

V

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A

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G

G

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Y

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L

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D

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I

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H

E

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H

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L

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M

V

I

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P

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x
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G

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E

active site: Y32 (= Y32), G34 (= G34), G35 (= G35), A36 (= A36), S37 (≠ I37), E58 (= E59), T81 (= T82), F120 (= F121), Q121 (= Q122), E122 (= E123), K170 (= K171), M265 (= M266), V292 (= V293), V399 (= V399), G425 (= G425), M427 (= M427), D452 (= D452), N479 (= N479), H481 (≠ F481), L482 (= L482), M484 (= M484), V485 (= V485), W488 (= W488), H557 (≠ K549)
binding methyl 2-{[(4-methylpyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: D290 (= D291), R291 (= R292), W488 (= W488), S567 (≠ A559)
binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R161), G221 (= G220), G222 (≠ Q221), G223 (= G222), T245 (= T246), L246 (= L247), M247 (≠ L248), L263 (= L264), G264 (= G265), M265 (= M266), H266 (= H267), G285 (= G286), R287 (= R288), D289 (= D290), R291 (= R292), D309 (≠ E310), I310 (= I311), G327 (= G328), D328 (≠ N329), V329 (= V330), M404 (= M404), G422 (= G422)
binding magnesium ion: D452 (= D452), N479 (= N479), H481 (≠ F481)
binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (= V399), G400 (= G400), Q401 (= Q401), H402 (= H402), M427 (= M427), G451 (= G451), D452 (= D452), G453 (= G453), S454 (≠ G454), N479 (= N479), H481 (≠ F481), L482 (= L482), G483 (= G483), M484 (= M484), V485 (= V485)

3e9yA Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron (see paper)
44% identity, 98% coverage: 13:569/569 of query aligns to 13:577/582 of 3e9yA

query
sites
3e9yA

G

G

S

A

E

D

A

I

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A

L

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Y

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A

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-

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A

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L

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L

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A

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A

A

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Q

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M

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T

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P

G

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L

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E

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H

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H

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L

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M

V

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A
x
S

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G

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G

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D

D

I

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I

L

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E

active site: Y32 (= Y32), G34 (= G34), G35 (= G35), A36 (= A36), S37 (≠ I37), E58 (= E59), T81 (= T82), F120 (= F121), Q121 (= Q122), E122 (= E123), K170 (= K171), M265 (= M266), V292 (= V293), V399 (= V399), G425 (= G425), M427 (= M427), D452 (= D452), N479 (= N479), H481 (≠ F481), L482 (= L482), M484 (= M484), V485 (= V485), W488 (= W488), H557 (≠ K549)
binding N-[(4-methylpyrimidin-2-yl)carbamoyl]-2-nitrobenzenesulfonamide: D290 (= D291), R291 (= R292), W488 (= W488), S567 (≠ A559)
binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R161), G221 (= G220), G222 (≠ Q221), G223 (= G222), T245 (= T246), L246 (= L247), M247 (≠ L248), L263 (= L264), G285 (= G286), R287 (= R288), D289 (= D290), R291 (= R292), D309 (≠ E310), I310 (= I311), G327 (= G328), D328 (≠ N329), V329 (= V330), M404 (= M404), G422 (= G422)
binding magnesium ion: D452 (= D452), N479 (= N479), H481 (≠ F481)
binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (= V399), G400 (= G400), Q401 (= Q401), H402 (= H402), M427 (= M427), G451 (= G451), G453 (= G453), S454 (≠ G454), N479 (= N479), H481 (≠ F481), L482 (= L482), G483 (= G483), M484 (= M484), V485 (= V485)

5k3sA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, bispyribac-sodium (see paper)
44% identity, 98% coverage: 13:569/569 of query aligns to 14:578/583 of 5k3sA

query
sites
5k3sA

G

G

S

A

E

D

A

I

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L

I

V

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E

S

A

L

L

L

E

R

R

E

Q

G

G

T

V

S

E

I

T

I

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F

F

G

A

Y
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Y

P

P

G
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G

A
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A

I
x
S

M

M

P

E

I

I

Y

H

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Q

A

A

L

L

H

-

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L

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H

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H

N

V

V

L

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R

H

H

E
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E

Q

Q

G

G

A

G

L

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H

F

A

A

E

E

G

G

Y

Y

A

A

K

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S

G

G

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K

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P

G

G

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I

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C

F

I

A

A

T
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T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

T

L

V

V

T

S

G

G

L

L

A

A

N

D

A

A

M

L

D

D

S

S

T

V

P

P

V

L

V

V

L

A

I

I

T

T

G

G

Q

Q

V

V

V

P

S

R

L

M

L

I

G

G

T

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

T

T

D

P

V

I

V

V

G

E

V

V

T

T

Q

R

P

S

V

I

T

T

K

K

W

H

N

N

Y

Y

Q

L

V

V

R

M

R

D

A

V

E

E

D

D

I

I

P

P

A

R

A

I

I

I

A

E

R

E

A

A

F

F

Y

F

I

L

A

A

R

T

S

S

G

G

R
|
R

P

P

G

G

P

P

V

V

V

L

I

V

D

D

I

V

T

P

K
|
K

D

D

A

I

Q

Q

I

Q

N

Q

Q

L

L

A

D

I

F

P

Y

N

Y

W

E

E

P

Q

V

A

K

M

S

R

I

L

R

P

S

G

Y

Y

-

M

-

S

-

R

T

M

P

P

E

K

Y

P

P

P

A

E

D

D

L

-

A

S

Q

H

I

L

E

E

E

Q

A

I

A

V

R

R

L

L

I

I

N

S

L

E

S

S

K

K

P

P

L

V

A

L

L

Y

V

V

G
|
G

Q
x
G

G
|
G

V

C

I

L

I

-

G

-

N

N

A

S

E

S

K

D

E

E

L

L

L

G

D

R

F

F

L

V

E

E

K

L

S

T

G

G

I

I

P

P

A

V

A

A

W

S

T
|
T

L
|
L

L
x
M

G

G

L

L

S

G

A

S

V

Y

P

P

S

C

D

D

H

D

P

E

L

L

N

S

V

L

G

H

M

M

L
|
L

G

G

M
|
M

H

H

G

G

N

T

Y

V

G

Y

P

A

N

N

I

Y

K

A

T

V

N

E

E

H

A

S

D

D

L

L

I

L

V

L

A

A

I

F

G
|
G

M

V

R
|
R

F

F

D
|
D

D

D

R
|
R

V
|
V

T

T

G

G

D

K

L

L

S

E

R

A

Y

F

A

A

T

S

N

R

A

A

K

K

I

I

I

V

H

H

M

I

E
x
D

I
|
I

D

D

P

S

A

A

E

E

I

I

N

G

K

K

N

N

V

K

H

T

A

P

D

H

V

V

P

S

I

V

L

C

G

G

N
x
D

V
|
V

K

K

K

L

T

A

L

L

P

Q

L

G

L

M

T

N

E

-

K

K

I

V

V

L

K

E

N

N

D

R

H

A

S

E

D

E

W

L

L

K

K

L

E

D

F

F

E

-

A

G

C

V

Q

W

R

R

V

N

E

E

Y

L

N

N

R

-

I

-

I

V

E

Q

K

K

Q

Q

L

K

N

F

P

P

I

L

E

S

K

F

G

K

I

-

T

T

M

F

G

G

E

E

V

A

V

I

A

P

K

P

-

Q

-

Y

-

A

-

I

-

K

-

V

V

L

S

D

Q

E

A

L

F

T

D

D

N

G

D

K

A

A

I

I

M

I

V

S

T

T

D

G

V
|
V

G
|
G

Q
|
Q

H
|
H

Q

Q

M
|
M

M

W

G

A

A

A

R

Q

Y

F

F

Y

K

N

F

Y

K

K

Q

K

S

P

R

R

S

Q

V

W

V

L

T

S

S

S

G
|
G

G

G

L

L

G
|
G

T

A

M
|
M

G

G

F

F

G

G

L

L

P

P

A

A

M

I

G

G

A

A

K

S

L

V

G

A

A

N

P

P

D

D

R

A

Q

I

V

V

C

V

V

D

F

I

V

D

G

G

D
|
D

G
|
G

G
x
S

L

F

Q

I

M

M

T

N

I

V

Q

Q

E

E

L

L

G

A

T

T

I

I

Y

R

Q

V

T

E

Q

N

I

L

P

P

V

V

K

K

I

V

V

L

L

L

N

N

N
|
N

N

Q

F
x
H

L
|
L

G
|
G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

E

D

L

R

F

F

F

Y

D

K

K

A

R

N

Y

R

A

A

S

H

T

T

E

F

L

L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

-

E

I

I

N

F

P

P

D

N

F

M

Q

L

T

L

I

F

A

A

K

A

G

A

Y

C

H

G

I

I

P

P

S

A

L

A

K

R

V

V

S

T

E

K

R

K

Q

A

D

D

L

L

D

R

E

E

A

A

I

I

Q

Q

K

T

M

M

V

L

E

D

A

T

D

P

G

G

P

P

F

Y

L

L

E

D

V

V

I

I

V

C

E

P

K
x
H

E

Q

G

E

N

H

V

V

F

L

P

P

M

M

V

I

P

P

A

S

G
|
G

A

G

S

T

V

F

S

N

D

D

I

V

R

I

L

T

E

E

active site: Y33 (= Y32), G35 (= G34), G36 (= G35), A37 (= A36), S38 (≠ I37), E59 (= E59), T82 (= T82), F121 (= F121), Q122 (= Q122), E123 (= E123), K171 (= K171), M266 (= M266), V293 (= V293), V400 (= V399), G426 (= G425), M428 (= M427), D453 (= D452), N480 (= N479), H482 (≠ F481), L483 (= L482), M485 (= M484), V486 (= V485), W489 (= W488), H558 (≠ K549)
binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: R292 (= R292), M485 (= M484), W489 (= W488), G569 (= G560)
binding flavin-adenine dinucleotide: R161 (= R161), G222 (= G220), G223 (≠ Q221), G224 (= G222), T246 (= T246), L247 (= L247), M248 (≠ L248), L264 (= L264), M266 (= M266), G286 (= G286), R288 (= R288), D290 (= D290), V293 (= V293), D310 (≠ E310), I311 (= I311), D329 (≠ N329), V330 (= V330), M405 (= M404), G423 (= G422)
binding magnesium ion: D453 (= D452), N480 (= N479), H482 (≠ F481)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V399), G401 (= G400), Q402 (= Q401), H403 (= H402), G426 (= G425), M428 (= M427), D453 (= D452), G454 (= G453), S455 (≠ G454), N480 (= N479), H482 (≠ F481), L483 (= L482), G484 (= G483), M485 (= M484), V486 (= V485)

9c4rA Acetolactate synthase, chloroplastic (see paper)
44% identity, 98% coverage: 13:569/569 of query aligns to 14:578/582 of 9c4rA

query
sites
9c4rA

G

G

S

A

E

D

A

I

V

L

I

V

Q

E

S

A

L

L

L

E

R

R

E

Q

G

G

T

V

S

E

I

T

I

V

F

F

G

A

Y

Y

P

P

G

G

A

A

I

S

M

M

P

E

I

I

Y

H

D

Q

A

A

L

L

H

-

D

T

H

R

Q

S

K

S

E

S

L

I

H

R

H

N

V

V

L

L

T

P

R

R

H

H

E

E

Q

Q

G

G

A

G

L

V

H

F

A

A

E

E

G

G

Y

Y

A

A

K

R

V

S

T

S

G

G

K

K

V

P

G

G

V

I

C

C

F

I

A

A

T

T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

T

L

V

V

T

S

G

G

L

L

A

A

N

D

A

A

M

L

D

D

S

S

T

V

P

P

V

L

V

V

L

A

I

I

T

T

G

G

Q

Q

V

V

V

P

S

R

L

M

L

I

G

G

T

T

D

D

A

A

F

F

Q

Q

E

E

T

T

D

P

V

I

V

V

G

E

V

V

T

T

Q

R

P

S

V

I

T

T

K

K

W

H

N

N

Y

Y

Q

L

V

V

R

M

R

D

A

V

E

E

D

D

I

I

P

P

A

R

A

I

I

I

A

E

R

E

A

A

F

F

Y

F

I

L

A

A

R

T

S

S

G

G

R
|
R

P

P

G

G

P

P

V

V

V

L

I

V

D

D

I

V

T

P

K

K

D

D

A

I

Q

Q

I

Q

N

Q

Q

L

L

A

D

I

F

P

Y

N

Y

W

E

E

P

Q

V

A

K

M

S

R

I

L

R

P

S

G

Y

Y

-

M

-

S

-

R

T

M

P

P

E

K

Y

P

P

P

A

E

D

D

L

-

A

S

Q

H

I

L

E

E

E

Q

A

I

A

V

R

R

L

L

I

I

N

S

L

E

S

S

K

K

P

P

L

V

A

L

L

Y

V

V

G
|
G

Q
x
G

G
|
G

V

C

I

L

I

-

G

-

N

N

A

S

E

S

K

D

E

E

L

L

L

G

D

R

F

F

L

V

E

E

K

L

S

T

G

G

I

I

P

P

A

V

A

A

W

S

T
|
T

L
|
L

L
x
M

G

G

L

L

S

G

A

S

V

Y

P

P

S

C

D

D

H

D

P

E

L

L

N

S

V

L

G

H

M

M

L
|
L

G

G

M
|
M

H
|
H

G

G

N

T

Y

V

G

Y

P

A

N

N

I

Y

K

A

T

V

N

E

E

H

A

S

D

D

L

L

I

L

V

L

A

A

I

F

G
|
G

M
x
V

R
|
R

F

F

D
|
D

D

D

R
|
R

V
|
V

T

T

G

G

D

K

L

L

S

E

R

A

Y

F

A

A

T

S

N

R

A

A

K

K

I

I

I

V

H

H

M

I

E
x
D

I
|
I

D

D

P

S

A

A

E

E

I

I

N

G

K

K

N

N

V

K

H

T

A

P

D

H

V

V

P

S

I

V

L

C

G

G

N
x
D

V
|
V

K

K

K

L

T

A

L

L

P

Q

L

G

L

M

T

N

E

-

K

K

I

V

V

L

K

E

N

N

D

R

H

A

S

E

D

E

W

L

L

K

K

L

E

D

F

F

E

-

A

G

C

V

Q

W

R

R

V

N

E

E

Y

L

N

N

R

-

I

-

I

V

E

Q

K

K

Q

Q

L

K

N

F

P

P

I

L

E

S

K

F

G

K

I

-

T

T

M

F

G

G

E

E

V

A

V

I

A

P

K

P

-

Q

-

Y

-

A

-

I

-

K

-

V

V

L

S

D

Q

E

A

L

F

T

D

D

N

G

D

K

A

A

I

I

M

I

V

S

T

T

D

G

V
|
V

G
|
G

Q
|
Q

H
|
H

Q

Q

M
|
M

M

W

G

A

A

A

R

Q

Y

F

F

Y

K

N

F

Y

K

K

Q

K

S

P

R

R

S

Q

V

W

V

L

T

S

S

S

G
|
G

G

G

L

L

G
|
G

T

A

M
|
M

G

G

F

F

G

G

L

L

P

P

A

A

M

I

G

G

A

A

K

S

L

V

G

A

A

N

P

P

D

D

R

A

Q

I

V

V

C

V

V

D

F

I

V

D

G
|
G

D
|
D

G
|
G

G
x
S

L

F

Q

I

M
|
M

T

N

I

V

Q

Q

E

E

L

L

G

A

T

T

I

I

Y

R

Q

V

T

E

Q

N

I

L

P

P

V

V

K

K

I

V

V

L

L

L

N

N

N
|
N

N

Q

F
x
H

L
|
L

G
|
G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

E

D

L

R

F

F

F

Y

D

K

K

A

R

N

Y

R

A

A

S

H

T

T

E

F

L

L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

-

E

I

I

N

F

P

P

D

N

F

M

Q

L

T

L

I

F

A

A

K

A

G

A

Y

C

H

G

I

I

P

P

S

A

L

A

K

R

V

V

S

T

E

K

R

K

Q

A

D

D

L

L

D

R

E

E

A

A

I

I

Q

Q

K

T

M

M

V

L

E

D

A

T

D

P

G

G

P

P

F

Y

L

L

E

D

V

V

I

I

V

C

E

P

K

H

E

Q

G

E

N

H

V

V

F

L

P

P

M

M

V

I

P

P

A
x
S

G

G

A

G

S

T

V

F

S

N

D

D

I

V

R

I

L

T

E

E

binding 2-(2-chloroethoxy)-N-[(4-methoxy-6-methylpyrimidin-2-yl)carbamoyl]benzene-1-sulfonamide: M266 (= M266), R292 (= R292), W489 (= W488), S568 (≠ A559)
binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V399), G401 (= G400), Q402 (= Q401), H403 (= H402), G426 (= G425), M428 (= M427), G452 (= G451), G454 (= G453), S455 (≠ G454), M458 (= M457), N480 (= N479), H482 (≠ F481), L483 (= L482), G484 (= G483), M485 (= M484), V486 (= V485)
binding flavin-adenine dinucleotide: R161 (= R161), G222 (= G220), G223 (≠ Q221), G224 (= G222), T246 (= T246), L247 (= L247), M248 (≠ L248), L264 (= L264), M266 (= M266), H267 (= H267), G286 (= G286), V287 (≠ M287), R288 (= R288), D290 (= D290), V293 (= V293), D310 (≠ E310), I311 (= I311), D329 (≠ N329), V330 (= V330), M405 (= M404), G423 (= G422)
binding magnesium ion: D453 (= D452), N480 (= N479), H482 (≠ F481)

9c4qA Acetolactate synthase, chloroplastic (see paper)
44% identity, 98% coverage: 13:569/569 of query aligns to 14:578/582 of 9c4qA

query
sites
9c4qA

G

G

S

A

E

D

A

I

V

L

I

V

Q

E

S

A

L

L

L

E

R

R

E

Q

G

G

T

V

S

E

I

T

I

V

F

F

G

A

Y

Y

P

P

G

G

A

A

I

S

M

M

P

E

I

I

Y

H

D

Q

A

A

L

L

H

-

D

T

H

R

Q

S

K

S

E

S

L

I

H

R

H

N

V

V

L

L

T

P

R

R

H

H

E

E

Q

Q

G

G

A

G

L

V

H

F

A

A

E

E

G

G

Y

Y

A

A

K

R

V

S

T

S

G

G

K

K

V

P

G

G

V

I

C

C

F

I

A

A

T

T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

T

L

V

V

T

S

G

G

L

L

A

A

N

D

A

A

M

L

D

D

S

S

T

V

P

P

V

L

V

V

L

A

I

I

T

T

G

G

Q

Q

V

V

V

P

S

R

L

M

L

I

G

G

T

T

D

D

A

A

F

F

Q

Q

E

E

T

T

D

P

V

I

V

V

G

E

V

V

T

T

Q

R

P

S

V

I

T

T

K

K

W

H

N

N

Y

Y

Q

L

V

V

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M

R

D

A

V

E

E

D

D

I

I

P

P

A

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A

I

I

I

A

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R

E

A

A

F

F

Y

F

I

L

A

A

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T

S

S

G

G

R
|
R

P

P

G

G

P

P

V

V

V

L

I

V

D

D

I

V

T

P

K

K

D

D

A

I

Q

Q

I

Q

N

Q

Q

L

L

A

D

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F

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Y

N

Y

W

E

E

P

Q

V

A

K

M

S

R

I

L

R

P

S

G

Y

Y

-

M

-

S

-

R

T

M

P

P

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K

Y

P

P

P

A

E

D

D

L

-

A

S

Q

H

I

L

E

E

E

Q

A

I

A

V

R

R

L

L

I

I

N

S

L

E

S

S

K

K

P

P

L

V

A

L

L

Y

V

V

G
|
G

Q
x
G

G
|
G

V

C

I

L

I

-

G

-

N

N

A

S

E

S

K

D

E

E

L

L

L

G

D

R

F

F

L

V

E

E

K

L

S

T

G

G

I

I

P

P

A

V

A

A

W

S

T
|
T

L
|
L

L
x
M

G

G

L

L

S

G

A

S

V

Y

P

P

S

C

D

D

H

D

P

E

L

L

N

S

V

L

G

H

M
|
M

L
|
L

G

G

M
|
M

H
|
H

G

G

N

T

Y

V

G

Y

P

A

N

N

I

Y

K

A

T

V

N

E

E

H

A

S

D

D

L

L

I

L

V

L

A

A

I

F

G
|
G

M
x
V

R
|
R

F

F

D
|
D

R
|
R

V
|
V

T

T

G

G

D

K

L

L

S

E

R

A

Y

F

A

A

T

S

N

R

A

A

K

K

I

I

I

V

H

H

M

I

E
x
D

I
|
I

D

D

P

S

A

A

E

E

I

I

N

G

K

K

N

N

V

K

H

T

A

P

D

H

V

V

P

S

I

V

L

C

G

G

N
x
D

V
|
V

K

K

K

L

T

A

L

L

P

Q

L

G

L

M

T

N

E

-

K

K

I

V

V

L

K

E

N

N

D

R

H

A

S

E

D

E

W

L

L

K

K

L

E

D

F

F

E

-

A

G

C

V

Q

W

R

R

V

N

E

E

Y

L

N

N

R

-

I

-

I

V

E

Q

K

K

Q

Q

L

K

N

F

P

P

I

L

E

S

K

F

G

K

I

-

T

T

M

F

G

G

E

E

V

A

V

I

A

P

K

P

-

Q

-
x
Y

-

A

-

I

-

K

-

V

V

L

S

D

Q

E

A

L

F

T

D

D

N

G

D

K

A

A

I

I

M

I

V

S

T

T

D

G

V
|
V

G
|
G

Q
|
Q

H
|
H

Q

Q

M
|
M

M

W

G

A

A

A

R

Q

Y

F

F

Y

K

N

F

Y

K

K

Q

K

S

P

R

R

S

Q

V

W

V

L

T

S

S

S

G
|
G

G

G

L

L

G
|
G

T

A

M
|
M

G

G

F

F

G

G

L

L

P

P

A

A

M

I

G

G

A

A

K

S

L

V

G

A

A

N

P

P

D

D

R

A

Q

I

V

V

C

V

V

D

F

I

V

D

G
|
G

D
|
D

G
|
G

G
x
S

L

F

Q

I

M
|
M

T

N

I

V

Q
|
Q

E

E

L

L

G

A

T

T

I

I

Y

R

Q

V

T

E

Q

N

I

L

P

P

V

V

K

K

I

V

V

L

L

L

N

N

N
|
N

N

Q

F
x
H

L
|
L

G
|
G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

E

D

L

R

F

F

F

Y

D

K

K

A

R

N

Y

R

A

A

S

H

T

T

E

F

L

L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

-

E

I

I

N

F

P

P

D

N

F

M

Q

L

T

L

I

F

A

A

K

A

G

A

Y

C

H

G

I

I

P

P

S

A

L

A

K

R

V

V

S

T

E

K

R
x
K

Q

A

D

D

L

L

D

R

E

E

A

A

I

I

Q

Q

K

T

M

M

V

L

E

D

A

T

D

P

G

G

P

P

F

Y

L

L

E

D

V

V

I

I

V

C

E

P

K

H

E

Q

G

E

N

H

V

V

F

L

P

P

M

M

V

I

P

P

A
x
S

G

G

A

G

S

T

V

F

S

N

D

D

I

V

R

I

L

T

E

E

binding 2-(2-fluoroethoxy)-N-[(4-methoxy-6-methylpyrimidin-2-yl)carbamoyl]benzene-1-sulfonamide: M266 (= M266), D291 (= D291), R292 (= R292), W489 (= W488), S568 (≠ A559)
binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V399), G401 (= G400), Q402 (= Q401), H403 (= H402), G426 (= G425), M428 (= M427), G452 (= G451), D453 (= D452), G454 (= G453), S455 (≠ G454), N480 (= N479), H482 (≠ F481), L483 (= L482), G484 (= G483), M485 (= M484), V486 (= V485)
binding flavin-adenine dinucleotide: R161 (= R161), G222 (= G220), G223 (≠ Q221), G224 (= G222), T246 (= T246), L247 (= L247), M248 (≠ L248), M263 (= M263), L264 (= L264), M266 (= M266), H267 (= H267), G286 (= G286), V287 (≠ M287), R288 (= R288), D290 (= D290), R292 (= R292), V293 (= V293), D310 (≠ E310), I311 (= I311), D329 (≠ N329), V330 (= V330), M405 (= M404), G423 (= G422)
binding magnesium ion: Y381 (vs. gap), D453 (= D452), N480 (= N479), H482 (≠ F481), K533 (≠ R524)
binding oxygen molecule: M458 (= M457), Q461 (= Q460)

9c4pA Acetolactate synthase, chloroplastic (see paper)
44% identity, 98% coverage: 13:569/569 of query aligns to 14:578/582 of 9c4pA

query
sites
9c4pA

G

G

S

A

E

D

A

I

V

L

I

V

Q

E

S

A

L

L

L

E

R

R

E

Q

G

G

T

V

S

E

I

T

I

V

F

F

G

A

Y

Y

P

P

G

G

A

A

I

S

M

M

P

E

I

I

Y

H

D

Q

A

A

L

L

H

-

D

T

H

R

Q

S

K

S

E

S

L

I

H

R

H

N

V

V

L

L

T

P

R

R

H

H

E

E

Q

Q

G

G

A

G

L

V

H

F

A

A

E

E

G

G

Y

Y

A

A

K

R

V

S

T

S

G

G

K

K

V

P

G

G

V

I

C

C

F

I

A

A

T

T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

T

L

V

V

T

S

G

G

L

L

A

A

N

D

A

A

M

L

D

D

S

S

T

V

P

P

V

L

V

V

L

A

I

I

T

T

G

G

Q

Q

V

V

V

P

S

R

L

M

L

I

G

G

T

T

D

D

A

A

F

F

Q

Q

E

E

T

T

D

P

V

I

V

V

G

E

V

V

T

T

Q

R

P

S

V

I

T

T

K

K

W

H

N

N

Y

Y

Q

L

V

V

R

M

R

D

A

V

E

E

D

D

I

I

P

P

A

R

A

I

I

I

A

E

R

E

A

A

F

F

Y

F

I

L

A

A

R

T

S

S

G

G

R
|
R

P

P

G

G

P

P

V

V

V

L

I

V

D

D

I

V

T

P

K

K

D

D

A

I

Q

Q

I

Q

N

Q

Q

L

L

A

D

I

F

P

Y

N

Y

W

E

E

P

Q

V

A

K

M

S

R

I

L

R

P

S

G

Y

Y

-

M

-

S

-

R

T

M

P

P

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K

Y

P

P

P

A

E

D

D

L

-

A

S

Q

H

I

L

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E

E

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A

I

A

V

R

R

L

L

I

I

N

S

L

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S

S

K

K

P

P

L

V

A

L

L

Y

V

V

G
|
G

Q
x
G

G
|
G

V

C

I

L

I

-

G

-

N

N

A

S

E

S

K

D

E

E

L

L

L

G

D

R

F

F

L

V

E

E

K

L

S

T

G

G

I

I

P

P

A

V

A

A

W

S

T
|
T

L
|
L

L
x
M

G

G

L

L

S

G

A

S

V

Y

P

P

S

C

D

D

H

D

P

E

L

L

N

S

V

L

G

H

M
|
M

L
|
L

G

G

M
|
M

H
|
H

G

G

N

T

Y

V

G

Y

P

A

N

N

I

Y

K

A

T

V

N

E

E

H

A

S

D

D

L

L

I

L

V

L

A

A

I

F

G
|
G

M
x
V

R
|
R

F

F

D
|
D

R
|
R

V
|
V

T

T

G

G

D

K

L

L

S

E

R

A

Y

F

A

A

T

S

N

R

A

A

K

K

I

I

I

V

H

H

M

I

E
x
D

I
|
I

D

D

P

S

A

A

E

E

I

I

N

G

K

K

N

N

V

K

H

T

A

P

D

H

V

V

P

S

I

V

L

C

G

G

N
x
D

V
|
V

K

K

K

L

T

A

L

L

P

Q

L

G

L

M

T

N

E

-

K

K

I

V

V

L

K

E

N

N

D

R

H

A

S

E

D

E

W

L

L

K

K

L

E

D

F

F

E

-

A

G

C

V

Q

W

R

R

V

N

E

E

Y

L

N

N

R

-

I

-

I

V

E

Q

K

K

Q

Q

L

K

N

F

P

P

I

L

E

S

K

F

G

K

I

-

T

T

M

F

G

G

E

E

V

A

V

I

A

P

K

P

-

Q

-

Y

-

A

-

I

-

K

-

V

V

L

S

D

Q

E

A

L

F

T

D

D

N

G

D

K

A

A

I

I

M

I

V

S

T

T

D

G

V
|
V

G
|
G

Q
|
Q

H
|
H

Q

Q

M
|
M

M

W

G

A

A

A

R

Q

Y

F

F

Y

K

N

F

Y

K

K

Q

K

S

P

R

R

S

Q

V

W

V

L

T

S

S

S

G
|
G

L

L

G
|
G

T

A

M
|
M

G

G

F

F

G

G

L

L

P

P

A

A

M

I

G

G

A

A

K

S

L

V

G

A

A

N

P

P

D

D

R

A

Q

I

V

V

C

V

V

D

F

I

V

D

G
|
G

D
|
D

G
|
G

G
x
S

L

F

Q

I

M
|
M

T

N

I

V

Q

Q

E

E

L

L

G

A

T

T

I

I

Y

R

Q

V

T

E

Q

N

I

L

P

P

V

V

K

K

I

V

V

L

L

L

N

N

N
|
N

N

Q

F
x
H

L
|
L

G
|
G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

E

D

L

R

F

F

F

Y

D

K

K

A

R

N

Y

R

A

A

S

H

T

T

E

F

L

L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

-

E

I

I

N

F

P

P

D

N

F

M

Q

L

T

L

I

F

A

A

K

A

G

A

Y

C

H

G

I

I

P

P

S

A

L

A

K

R

V

V

S

T

E

K

R

K

Q

A

D

D

L

L

D

R

E

E

A

A

I

I

Q

Q

K

T

M

M

V

L

E

D

A

T

D

P

G

G

P

P

F

Y

L

L

E

D

V

V

I

I

V

C

E

P

K

H

E

Q

G

E

N

H

V

V

F

L

P

P

M

M

V

I

P

P

A
x
S

G

G

A

G

S

T

V

F

S

N

D

D

I

V

R

I

L

T

E

E

binding 2-(2-chloroethoxy)-N-[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)carbamoyl]benzene-1-sulfonamide: M266 (= M266), D291 (= D291), R292 (= R292), M485 (= M484), W489 (= W488), S568 (≠ A559)
binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V399), G401 (= G400), Q402 (= Q401), H403 (= H402), G426 (= G425), M428 (= M427), G452 (= G451), D453 (= D452), G454 (= G453), S455 (≠ G454), M458 (= M457), N480 (= N479), H482 (≠ F481), L483 (= L482), G484 (= G483), M485 (= M484), V486 (= V485)
binding flavin-adenine dinucleotide: R161 (= R161), G222 (= G220), G223 (≠ Q221), G224 (= G222), T246 (= T246), L247 (= L247), M248 (≠ L248), M263 (= M263), L264 (= L264), M266 (= M266), H267 (= H267), G286 (= G286), V287 (≠ M287), R288 (= R288), D290 (= D290), R292 (= R292), V293 (= V293), D310 (≠ E310), I311 (= I311), D329 (≠ N329), V330 (= V330), M405 (= M404), G423 (= G422), G424 (= G423)
binding magnesium ion: D453 (= D452), N480 (= N479), H482 (≠ F481)

8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
44% identity, 98% coverage: 13:569/569 of query aligns to 14:578/582 of 8et4A

query
sites
8et4A

G

G

S

A

E

D

A

I

V

L

I

V

Q

E

S

A

L

L

L

E

R

R

E

Q

G

G

T

V

S

E

I

T

I

V

F

F

G

A

Y

Y

P

P

G

G

A

A

I

S

M

M

P

E

I

I

Y

H

D

Q

A

A

L

L

H

-

D

T

H

R

Q

S

K

S

E

S

L

I

H

R

H

N

V

V

L

L

T

P

R

R

H

H

E

E

Q

Q

G

G

A

G

L

V

H

F

A

A

E

E

G

G

Y

Y

A

A

K

R

V

S

T

S

G

G

K

K

V

P

G

G

V

I

C

C

F

I

A

A

T

T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

T

L

V

V

T

S

G

G

L

L

A

A

N

D

A

A

M

L

D

D

S

S

T

V

P

P

V

L

V

V

L

A

I

I

T

T

G

G

Q

Q

V

V

V

P

S

R

L

M

L

I

G

G

T

T

D

D

A

A

F

F

Q

Q

E

E

T

T

D

P

V

I

V

V

G

E

V

V

T

T

Q

R

P

S

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I

T

T

K

K

W

H

N

N

Y

Y

Q

L

V

V

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A

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E

D

D

I

I

P

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A

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A

I

I

I

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A

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A

A

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R

P

P

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G

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P

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V

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L

I

V

D

D

I

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K

K

D

D

A

I

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I

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N

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Q

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L

A

D

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Y

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Y

W

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A

K

M

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I

L

R

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G

Y

Y

-

M

-

S

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R

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M

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K

Y

P

P

P

A

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D

D

L

-

A

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Q

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I

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E

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A

I

A

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G
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G

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x
G

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G

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C

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L

I

-

G

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N

N

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T

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L

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x
M

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A

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Y

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L

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M

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H

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Y

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A

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x
V

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x
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I

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V

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G

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N

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-

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N

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-

A

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C

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E

E

Y

L

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N

R

-

I

-

I

V

E

Q

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K

Q

Q

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P

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F

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K

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M

F

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G

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A

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P

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Y

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A

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I

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K

-

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V

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A

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H

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M

M

W

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A

A

A

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F

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Y

K

N

F

Y

K

K

Q

K

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R

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Q

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G

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M

G

G

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F

G

G

L

L

P

P

A

A

M

I

G

G

A

A

K

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L

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G

A

A

N

P

P

D

D

R

A

Q

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V

C

V

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F

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G

D
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D

G
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G

G
x
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M

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Q

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E

L

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A

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R

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V

T

E

Q

N

I

L

P

P

V

V

K

K

I

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V

L

L

L

N

N

N
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N

N

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x
H

L
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L

G
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G

M
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M

V
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V

R

M

Q

Q

W
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W

Q

E

E

D

L

R

F

F

F

Y

D

K

K

A

R

N

Y

R

A

A

S

H

T

T

E

F

L

L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

-

E

I

I

N

F

P

P

D

N

F

M

Q

L

T

L

I

F

A

A

K

A

G

A

Y

C

H

G

I

I

P

P

S

A

L

A

K

R

V

V

S

T

E

K

R
x
K

Q

A

D

D

L

L

D

R

E

E

A

A

I

I

Q

Q

K

T

M

M

V

L

E

D

A

T

D

P

G

G

P

P

F

Y

L

L

E

D

V

V

I

I

V

C

E

P

K

H

E

Q

G

E

N

H

V

V

F

L

P

P

M

M

V

I

P

P

A
x
S

G

G

A

G

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F

S

N

D

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I

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E

binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V399), G401 (= G400), Q402 (= Q401), H403 (= H402), G426 (= G425), M428 (= M427), G452 (= G451), D453 (= D452), G454 (= G453), S455 (≠ G454), M458 (= M457), N480 (= N479), H482 (≠ F481), L483 (= L482), G484 (= G483), M485 (= M484), V486 (= V485)
binding flavin-adenine dinucleotide: R161 (= R161), G222 (= G220), G223 (≠ Q221), G224 (= G222), T246 (= T246), L247 (= L247), M248 (≠ L248), L264 (= L264), M266 (= M266), H267 (= H267), G286 (= G286), V287 (≠ M287), R288 (= R288), D290 (= D290), R292 (= R292), V293 (= V293), D310 (≠ E310), I311 (= I311), D329 (≠ N329), V330 (= V330), M405 (= M404), G423 (= G422)
binding magnesium ion: F370 (≠ K374), D453 (= D452), M458 (= M457), Q461 (= Q460), N480 (= N479), H482 (≠ F481), K533 (≠ R524)
binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: M266 (= M266), R292 (= R292), M485 (= M484), W489 (= W488), S568 (≠ A559)

5wj1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a triazolopyrimidine herbicide, penoxsulam (see paper)
44% identity, 98% coverage: 13:569/569 of query aligns to 14:578/582 of 5wj1A

query
sites
5wj1A

G

G

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A

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N

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-

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A

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R

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E

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N

I

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K

I

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L

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N
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x
H

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|
L

G
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G

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M

V
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V

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M

Q

Q

W
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W

Q

E

E

D

L

R

F

F

F

Y

D

K

K

A

R

N

Y

R

A

A

S

H

T

T

E

F

L

L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

-

E

I

I

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F

P

P

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N

F

M

Q

L

T

L

I

F

A

A

K

A

G

A

Y

C

H

G

I

I

P

P

S

A

L

A

K

R

V

V

S

T

E

K

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K

Q

A

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D

L

L

D

R

E

E

A

A

I

I

Q

Q

K

T

M

M

V

L

E

D

A

T

D

P

G

G

P

P

F

Y

L

L

E

D

V

V

I

I

V

C

E

P

K
x
H

E

Q

G

E

N

H

V

V

F

L

P

P

M

M

V

I

P

P

A
x
S

G

G

A

G

S

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V

F

S

N

D

D

I

V

R

I

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T

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E

active site: Y33 (= Y32), G35 (= G34), G36 (= G35), A37 (= A36), S38 (≠ I37), E59 (= E59), T82 (= T82), F121 (= F121), Q122 (= Q122), E123 (= E123), K171 (= K171), M266 (= M266), V293 (= V293), V400 (= V399), G426 (= G425), M428 (= M427), D453 (= D452), N480 (= N479), H482 (≠ F481), L483 (= L482), M485 (= M484), V486 (= V485), W489 (= W488), H558 (≠ K549)
binding flavin-adenine dinucleotide: R161 (= R161), G222 (= G220), G223 (≠ Q221), G224 (= G222), T246 (= T246), L247 (= L247), M248 (≠ L248), M263 (= M263), L264 (= L264), G286 (= G286), R288 (= R288), V293 (= V293), D310 (≠ E310), I311 (= I311), D329 (≠ N329), V330 (= V330), M405 (= M404), G423 (= G422), G424 (= G423)
binding magnesium ion: D453 (= D452), N480 (= N479), H482 (≠ F481)
binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M266 (= M266), D291 (= D291), R292 (= R292), M485 (= M484), W489 (= W488), S568 (≠ A559)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V399), G401 (= G400), Q402 (= Q401), H403 (= H402), M428 (= M427), D453 (= D452), G454 (= G453), S455 (≠ G454), M458 (= M457), N480 (= N479), H482 (≠ F481), L483 (= L482), G484 (= G483), M485 (= M484), V486 (= V485)

5k6tA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, propoxycarbazone-sodium (see paper)
44% identity, 98% coverage: 13:569/569 of query aligns to 14:578/582 of 5k6tA

query
sites
5k6tA

G

G

S

A

E

D

A

I

V

L

I

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Q

E

S

A

L

L

L

E

R

R

E

Q

G

G

T

V

S

E

I

T

I

V

F

F

G

A

Y
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Y

P

P

G
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G

A
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A

I
x
S

M

M

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E

I

I

Y

H

D

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A

A

L

L

H

-

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E

S

L

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H

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H

N

V

V

L

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R

R

H

H

E
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E

Q

Q

G

G

A

G

L

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H

F

A

A

E

E

G

G

Y

Y

A

A

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S

G

G

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K

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G

G

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C

F

I

A

A

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T

S

S

G

G

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P

G

G

A

A

T

T

N

N

T

L

V

V

T

S

G

G

L

L

A

A

N

D

A

A

M

L

D

D

S

S

T

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P

P

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L

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A

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I

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T

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G

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I

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E

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D

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K

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x
G

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L

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H

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V

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N

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-

K

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N

N

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Q

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Y

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A

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I

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M

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Y

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V

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G

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G

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L

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P

A

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G

A

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A

A

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C

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G

D
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D

G
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x
S

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M

T

N

I

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Q

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E

L

L

G

A

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I

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N

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P

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K

I

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L

L

N

N

N
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x
H

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L

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G

M
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M

V
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V

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W

Q

E

E

D

L

R

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F

F

Y

D

K

K

A

R

N

Y

R

A

A

S

H

T

T

E

F

L

L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

-

E

I

I

N

F

P

P

D

N

F

M

Q

L

T

L

I

F

A

A

K

A

G

A

Y

C

H

G

I

I

P

P

S

A

L

A

K

R

V

V

S

T

E

K

R

K

Q

A

D

D

L

L

D

R

E

E

A

A

I

I

Q

Q

K

T

M

M

V

L

E

D

A

T

D

P

G

G

P

P

F

Y

L

L

E

D

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V

I

I

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C

E

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K
x
H

E

Q

G

E

N

H

V

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L

P

P

M

M

V

I

P

P

A
x
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G

A

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D

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I

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E

E

active site: Y33 (= Y32), G35 (= G34), G36 (= G35), A37 (= A36), S38 (≠ I37), E59 (= E59), T82 (= T82), F121 (= F121), Q122 (= Q122), E123 (= E123), K171 (= K171), M266 (= M266), V293 (= V293), V400 (= V399), G426 (= G425), M428 (= M427), D453 (= D452), N480 (= N479), H482 (≠ F481), L483 (= L482), M485 (= M484), V486 (= V485), W489 (= W488), H558 (≠ K549)
binding methyl 2-[(4-methyl-5-oxidanylidene-3-propoxy-1,2,4-triazol-1-yl)carbonylsulfamoyl]benzoate: H267 (= H267), R292 (= R292), M485 (= M484), W489 (= W488), S568 (≠ A559)
binding flavin-adenine dinucleotide: R161 (= R161), G222 (= G220), G223 (≠ Q221), G224 (= G222), T246 (= T246), L247 (= L247), M248 (≠ L248), L264 (= L264), G286 (= G286), R288 (= R288), D290 (= D290), R292 (= R292), V293 (= V293), D310 (≠ E310), I311 (= I311), D329 (≠ N329), V330 (= V330), Q404 (= Q403), M405 (= M404), G423 (= G422)
binding magnesium ion: D453 (= D452), N480 (= N479), H482 (≠ F481)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (= V399), G401 (= G400), Q402 (= Q401), H403 (= H402), G426 (= G425), M428 (= M427), G452 (= G451), G454 (= G453), S455 (≠ G454), N480 (= N479), H482 (≠ F481), L483 (= L482), G484 (= G483)

5k6rA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, thiencarbazone-methyl (see paper)
44% identity, 98% coverage: 13:569/569 of query aligns to 14:578/582 of 5k6rA

query
sites
5k6rA

G

G

S

A

E

D

A

I

V

L

I

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Q

E

S

A

L

L

L

E

R

R

E

Q

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Y

P

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A
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I
x
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I

Y

H

D

Q

A

A

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L

H

-

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I

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E

Q

Q

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G

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A

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G

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Y

A

A

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G

G

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T

S

S

G

G

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P

G

G

A

A

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N

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G

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K

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L

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N

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-

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-

I

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-

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G

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T

N

I

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E

E

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L

G

A

T

T

I

I

Y

R

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V

T

E

Q

N

I

L

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P

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V

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K

I

V

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L

L

L

N

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N
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N

Q

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x
H

L
|
L

G
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G

M
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M

V
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V

R

M

Q

Q

W
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W

Q

E

E

D

L

R

F

F

F

Y

D

K

K

A

R

N

Y

R

A

A

S

H

T

T

E

F

L

L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

-

E

I

I

N

F

P

P

D

N

F

M

Q

L

T

L

I

F

A

A

K

A

G

A

Y

C

H

G

I

I

P

P

S

A

L

A

K

R

V

V

S

T

E

K

R

K

Q

A

D

D

L

L

D

R

E

E

A

A

I

I

Q

Q

K

T

M

M

V

L

E

D

A

T

D

P

G

G

P

P

F

Y

L

L

E

D

V

V

I

I

V

C

E

P

K
x
H

E

Q

G

E

N

H

V

V

F

L

P

P

M

M

V

I

P

P

A
x
S

G

G

A

G

S

T

V

F

S

N

D

D

I

V

R

I

L

T

E

E

active site: Y33 (= Y32), G35 (= G34), G36 (= G35), A37 (= A36), S38 (≠ I37), E59 (= E59), T82 (= T82), F121 (= F121), Q122 (= Q122), E123 (= E123), K171 (= K171), M266 (= M266), V293 (= V293), V400 (= V399), G426 (= G425), M428 (= M427), D453 (= D452), N480 (= N479), H482 (≠ F481), L483 (= L482), M485 (= M484), V486 (= V485), W489 (= W488), H558 (≠ K549)
binding methyl 4-[(3-methoxy-4-methyl-5-oxidanylidene-1,2,4-triazol-1-yl)carbonylsulfamoyl]-5-methyl-thiophene-3-carboxylate: R292 (= R292), W489 (= W488), S568 (≠ A559)
binding flavin-adenine dinucleotide: R161 (= R161), G222 (= G220), G223 (≠ Q221), G224 (= G222), T246 (= T246), L247 (= L247), M248 (≠ L248), L264 (= L264), M266 (= M266), G286 (= G286), R288 (= R288), R292 (= R292), V293 (= V293), D310 (≠ E310), I311 (= I311), G328 (= G328), D329 (≠ N329), V330 (= V330), M405 (= M404), G423 (= G422)
binding magnesium ion: D453 (= D452), N480 (= N479), H482 (≠ F481)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (= V399), G401 (= G400), Q402 (= Q401), H403 (= H402), G426 (= G425), M428 (= M427), D453 (= D452), G454 (= G453), S455 (≠ G454), M458 (= M457), N480 (= N479), H482 (≠ F481), L483 (= L482), G484 (= G483), M485 (= M484), V486 (= V485)

1z8nA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with an imidazolinone herbicide, imazaquin (see paper)
44% identity, 98% coverage: 13:569/569 of query aligns to 14:578/582 of 1z8nA

query
sites
1z8nA

G

G

S

A

E

D

A

I

V

L

I

V

Q

E

S

A

L

L

L

E

R

R

E

Q

G

G

T

V

S

E

I

T

I

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F

F

G

A

Y
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Y

P

P

G
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G

A
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A

I
x
S

M

M

P

E

I

I

Y

H

D

Q

A

A

L

L

H

-

D

T

H

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K

S

E

S

L

I

H

R

H

N

V

V

L

L

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R

R

H

H

E
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E

Q

Q

G

G

A

G

L

V

H

F

A

A

E

E

G

G

Y

Y

A

A

K

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active site: Y33 (= Y32), G35 (= G34), G36 (= G35), A37 (= A36), S38 (≠ I37), E59 (= E59), T82 (= T82), F121 (= F121), Q122 (= Q122), E123 (= E123), K171 (= K171), M266 (= M266), V293 (= V293), V400 (= V399), G426 (= G425), M428 (= M427), D453 (= D452), N480 (= N479), H482 (≠ F481), L483 (= L482), M485 (= M484), V486 (= V485), W489 (= W488), H558 (≠ K549)
binding 2-(4-isopropyl-4-methyl-5-oxo-4,5-dihydro-1h-imidazol-2-yl)quinoline-3-carboxylic acid: K135 (= K135), R161 (= R161), Y191 (= Y191), R194 (vs. gap), D291 (= D291), R292 (= R292), D312 (= D312), W489 (= W488), G569 (= G560)
binding flavin-adenine dinucleotide: R161 (= R161), G222 (= G220), G224 (= G222), T246 (= T246), L247 (= L247), M248 (≠ L248), L264 (= L264), G265 (= G265), M266 (= M266), H267 (= H267), G286 (= G286), V287 (≠ M287), R288 (= R288), D290 (= D290), R292 (= R292), V293 (= V293), D310 (≠ E310), I311 (= I311), D329 (≠ N329), V330 (= V330), M405 (= M404), G423 (= G422), G424 (= G423)
binding magnesium ion: D453 (= D452), N480 (= N479)
binding thiamine diphosphate: V400 (= V399), G401 (= G400), Q402 (= Q401), H403 (= H402), G426 (= G425), M428 (= M427), G452 (= G451), G454 (= G453), S455 (≠ G454), N480 (= N479), H482 (≠ F481), L483 (= L482), G484 (= G483), M485 (= M484), V486 (= V485)

1yi1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, tribenuron methyl (see paper)
44% identity, 98% coverage: 13:569/569 of query aligns to 14:578/582 of 1yi1A

query
sites
1yi1A

G

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A

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E

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H

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M

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A
x
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E

active site: Y33 (= Y32), G35 (= G34), G36 (= G35), A37 (= A36), S38 (≠ I37), E59 (= E59), T82 (= T82), F121 (= F121), Q122 (= Q122), E123 (= E123), K171 (= K171), M266 (= M266), V293 (= V293), V400 (= V399), G426 (= G425), M428 (= M427), D453 (= D452), N480 (= N479), H482 (≠ F481), L483 (= L482), M485 (= M484), V486 (= V485), W489 (= W488), H558 (≠ K549)
binding methyl 2-[4-methoxy-6-methyl-1,3,5-trazin-2-yl(methyl)carbamoylsulfamoyl]benzoate: D291 (= D291), R292 (= R292), W489 (= W488), S568 (≠ A559)
binding flavin-adenine dinucleotide: R161 (= R161), G223 (≠ Q221), G224 (= G222), T246 (= T246), L247 (= L247), M248 (≠ L248), M263 (= M263), L264 (= L264), G265 (= G265), M266 (= M266), H267 (= H267), G286 (= G286), V287 (≠ M287), R288 (= R288), D290 (= D290), V293 (= V293), D310 (≠ E310), I311 (= I311), D329 (≠ N329), V330 (= V330), M405 (= M404), G423 (= G422), G424 (= G423)
binding magnesium ion: D453 (= D452), N480 (= N479), H482 (≠ F481)

1yi0A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, sulfometuron methyl (see paper)
44% identity, 98% coverage: 13:569/569 of query aligns to 14:578/582 of 1yi0A

query
sites
1yi0A

G

G

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A

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A
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x
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A

K

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S

G

G

K

K

V

P

G

G

V

I

C

C

F

I

A

A

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|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

T

L

V

V

T

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G

G

L

L

A

A

N

D

A

A

M

L

D

D

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S

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P

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L

V

V

L

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I

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G

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Q

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V

V

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L

M

L

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G

G

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D

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|
F

Q
|
Q

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|
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I

V

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K

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A

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F

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|
R

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G

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V

V

L

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D

I

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|
K

D

D

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Q

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L

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G

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G

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N

N

A

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L

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G

G

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I

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T

L
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L

L
x
M

G

G

L

L

S

G

A

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Y

P

P

S

C

D

D

H

D

P

E

L

L

N

S

V

L

G

H

M

M

L
|
L

G
|
G

M
|
M

H
|
H

G

G

N

T

Y

V

G

Y

P

A

N

N

I

Y

K

A

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V

N

E

E

H

A

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D

D

L

L

I

L

V

L

A

A

I

F

G
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G

M
x
V

R
|
R

F

F

D
|
D

R
|
R

V
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V

T

T

G

G

D

K

L

L

S

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A

Y

F

A

A

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S

N

R

A

A

K

K

I

I

I

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H

H

M

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x
D

I
|
I

D

D

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A

A

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E

I

I

N

G

K

K

N

N

V

K

H

T

A

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D

H

V

V

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S

I

V

L

C

G
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G

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x
D

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V

K

K

K

L

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A

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L

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L

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N

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K

K

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V

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N

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A

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D

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K

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A

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W

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S

G
|
G

L

L

G
|
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|
M

G

G

F

F

G

G

L

L

P

P

A

A

M

I

G

G

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A

K

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L

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A

A

N

P

P

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D

R

A

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|
D

G

G

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M

M

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N

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V

Q

Q

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E

L

L

G

A

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T

I

I

Y

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Q

N

I

L

P

P

V

V

K

K

I

V

V

L

L

L

N

N

N
|
N

N

Q

F
x
H

L
|
L

G

G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

E

D

L

R

F

F

F

Y

D

K

K

A

R

N

Y

R

A

A

S

H

T

T

E

F

L

L

-

G

-

D

-

P

-

A

-

Q

-

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-

D

-

E

I

I

N

F

P

P

D

N

F

M

Q

L

T

L

I

F

A

A

K

A

G

A

Y

C

H

G

I

I

P

P

S

A

L

A

K

R

V

V

S

T

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K

R

K

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A

D

D

L

L

D

R

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A

A

I

I

Q

Q

K

T

M

M

V

L

E

D

A

T

D

P

G

G

P

P

F

Y

L

L

E

D

V

V

I

I

V

C

E

P

K
x
H

E

Q

G

E

N

H

V

V

F

L

P

P

M

M

V

I

P

P

A
x
S

G

G

A

G

S

T

V

F

S

N

D

D

I

V

R

I

L

T

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E

active site: Y33 (= Y32), G35 (= G34), G36 (= G35), A37 (= A36), S38 (≠ I37), E59 (= E59), T82 (= T82), F121 (= F121), Q122 (= Q122), E123 (= E123), K171 (= K171), M266 (= M266), V293 (= V293), V400 (= V399), G426 (= G425), M428 (= M427), D453 (= D452), N480 (= N479), H482 (≠ F481), L483 (= L482), M485 (= M484), V486 (= V485), W489 (= W488), H558 (≠ K549)
binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (= D291), R292 (= R292), W489 (= W488), S568 (≠ A559)
binding flavin-adenine dinucleotide: R161 (= R161), G222 (= G220), G223 (≠ Q221), G224 (= G222), T246 (= T246), L247 (= L247), M248 (≠ L248), L264 (= L264), G265 (= G265), M266 (= M266), H267 (= H267), G286 (= G286), V287 (≠ M287), R288 (= R288), D290 (= D290), R292 (= R292), V293 (= V293), D310 (≠ E310), I311 (= I311), G328 (= G328), D329 (≠ N329), V330 (= V330), M405 (= M404), G423 (= G422), G424 (= G423)
binding magnesium ion: D453 (= D452), N480 (= N479), H482 (≠ F481)

1yhzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron (see paper)
44% identity, 98% coverage: 13:569/569 of query aligns to 14:578/582 of 1yhzA

query
sites
1yhzA

G

G

S

A

E

D

A

I

V

L

I

V

Q

E

S

A

L

L

L

E

R

R

E

Q

G

G

T

V

S

E

I

T

I

V

F

F

G

A

Y
|
Y

P

P

G
|
G

A
|
A

I
x
S

M

M

P

E

I

I

Y

H

D

Q

A

A

L

L

H

-

D

T

H

R

Q

S

K

S

E

S

L

I

H

R

H

N

V

V

L

L

T

P

R

R

H

H

E
|
E

Q

Q

G

G

A

G

L

V

H

F

A

A

E

E

G

G

Y

Y

A

A

K

R

V

S

T

S

G

G

K

K

V

P

G

G

V

I

C

C

F

I

A

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

T

L

V

V

T

S

G

G

L

L

A

A

N

D

A

A

M

L

D

D

S

S

T

V

P

P

V

L

V

V

L

A

I

I

T

T

G

G

Q

Q

V

V

V

P

S

R

L

M

L

I

G

G

T

T

D

D

A

A

F
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F

Q
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Q

E
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E

T

T

D

P

V

I

V

V

G

E

V

V

T

T

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R

P

S

V

I

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T

K

K

W

H

N

N

Y

Y

Q

L

V

V

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M

R

D

A

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E

E

D

D

I

I

P

P

A

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A

I

I

I

A

E

R

E

A

A

F

F

Y

F

I

L

A

A

R

T

S

S

G

G

R
|
R

P

P

G

G

P

P

V

V

V

L

I

V

D

D

I

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K
|
K

D

D

A

I

Q

Q

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Q

N

Q

Q

L

L

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K

K

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P

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A

L

L

Y

V

V

G

G

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x
G

G
|
G

V

C

I

L

I

-

G

-

N

N

A

S

E

S

K

D

E

E

L

L

L

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F

F

L

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E

K

L

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G

G

I

I

P

P

A

V

A

A

W

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|
T

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|
L

L
x
M

G

G

L

L

S

G

A

S

V

Y

P

P

S

C

D

D

H

D

P

E

L

L

N

S

V

L

G

H

M

M

L
|
L

G

G

M
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M

H
|
H

G

G

N

T

Y

V

G

Y

P

A

N

N

I

Y

K

A

T

V

N

E

E

H

A

S

D

D

L

L

I

L

V

L

A

A

I

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G
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G

M
x
V

R
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R

F

F

D
|
D

R
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R

V
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V

T

T

G

G

D

K

L

L

S

E

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A

Y

F

A

A

T

S

N

R

A

A

K

K

I

I

I

V

H

H

M

I

E
x
D

I
|
I

D

D

P

S

A

A

E

E

I

I

N

G

K

K

N

N

V

K

H

T

A

P

D

H

V

V

P

S

I

V

L

C

G

G

N
x
D

V
|
V

K

K

K

L

T

A

L

L

P

Q

L

G

L

M

T

N

E

-

K

K

I

V

V

L

K

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N

N

D

R

H

A

S

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D

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L

L

K

K

L

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D

F

F

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A

G

C

V

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W

R

R

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N

E

E

Y

L

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N

R

-

I

-

I

V

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K

K

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Q

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K

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T

T

M

F

G

G

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A

V

I

A

P

K

P

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Q

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Y

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A

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I

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K

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V

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A

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D

N

G

D

K

A

A

I

I

M

I

V

S

T

T

D

G

V
|
V

G

G

Q

Q

H

H

Q
|
Q

M
|
M

M

W

G

A

A

A

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Y

F

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N

F

Y

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K

Q

K

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P

R

R

S

Q

V

W

V

L

T

S

S

S

G
|
G

L

L

G
|
G

T

A

M
|
M

G

G

F

F

G

G

L

L

P

P

A

A

M

I

G

G

A

A

K

S

L

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G

A

A

N

P

P

D

D

R

A

Q

I

V

V

C

V

V

D

F

I

V

D

G

G

D
|
D

G

G

G

S

L

F

Q

I

M

M

T

N

I

V

Q

Q

E

E

L

L

G

A

T

T

I

I

Y

R

Q

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T

E

Q

N

I

L

P

P

V

V

K

K

I

V

V

L

L

L

N

N

N
|
N

N

Q

F
x
H

L
|
L

G

G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

E

D

L

R

F

F

F

Y

D

K

K

A

R

N

Y

R

A

A

S

H

T

T

E

F

L

L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

-

E

I

I

N

F

P

P

D

N

F

M

Q

L

T

L

I

F

A

A

K

A

G

A

Y

C

H

G

I

I

P

P

S

A

L

A

K

R

V

V

S

T

E

K

R

K

Q

A

D

D

L

L

D

R

E

E

A

A

I

I

Q

Q

K

T

M

M

V

L

E

D

A

T

D

P

G

G

P

P

F

Y

L

L

E

D

V

V

I

I

V

C

E

P

K
x
H

E

Q

G

E

N

H

V

V

F

L

P

P

M

M

V

I

P

P

A
x
S

G

G

A

G

S

T

V

F

S

N

D

D

I

V

R

I

L

T

E

E

active site: Y33 (= Y32), G35 (= G34), G36 (= G35), A37 (= A36), S38 (≠ I37), E59 (= E59), T82 (= T82), F121 (= F121), Q122 (= Q122), E123 (= E123), K171 (= K171), M266 (= M266), V293 (= V293), V400 (= V399), G426 (= G425), M428 (= M427), D453 (= D452), N480 (= N479), H482 (≠ F481), L483 (= L482), M485 (= M484), V486 (= V485), W489 (= W488), H558 (≠ K549)
binding 1-(2-chlorophenylsulfonyl)-3-(4-methoxy-6-methyl-l,3,5-triazin-2-yl)urea: D291 (= D291), R292 (= R292), M485 (= M484), W489 (= W488), S568 (≠ A559)
binding flavin-adenine dinucleotide: R161 (= R161), G223 (≠ Q221), G224 (= G222), T246 (= T246), L247 (= L247), M248 (≠ L248), L264 (= L264), M266 (= M266), H267 (= H267), G286 (= G286), V287 (≠ M287), R288 (= R288), D290 (= D290), V293 (= V293), D310 (≠ E310), I311 (= I311), D329 (≠ N329), V330 (= V330), Q404 (= Q403), M405 (= M404), G423 (= G422), G424 (= G423)
binding magnesium ion: D453 (= D452), N480 (= N479), H482 (≠ F481)

1yhyA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
44% identity, 98% coverage: 13:569/569 of query aligns to 14:578/582 of 1yhyA

query
sites
1yhyA

G

G

S

A

E

D

A

I

V

L

I

V

Q

E

S

A

L

L

L

E

R

R

E

Q

G

G

T

V

S

E

I

T

I

V

F

F

G

A

Y
|
Y

P

P

G
|
G

A
|
A

I
x
S

M

M

P

E

I

I

Y

H

D

Q

A

A

L

L

H

-

D

T

H

R

Q

S

K

S

E

S

L

I

H

R

H

N

V

V

L

L

T

P

R

R

H

H

E
|
E

Q

Q

G

G

A

G

L

V

H

F

A

A

E

E

G

G

Y

Y

A

A

K

R

V

S

T

S

G

G

K

K

V

P

G

G

V

I

C

C

F

I

A

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

T

L

V

V

T

S

G

G

L

L

A

A

N

D

A

A

M

L

D

D

S

S

T

V

P

P

V

L

V

V

L

A

I

I

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T

G

G

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Q

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F

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E
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E

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I

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G

E

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V

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K

W

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N

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Y

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A

V

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D

D

I

I

P

P

A

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A

I

I

I

A

E

R

E

A

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F

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Y

F

I

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A

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T

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S

G

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R

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G

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P

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V

V

L

I

V

D

D

I

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K

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A

D

I

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P

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N

Y

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E

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A

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I

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S

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Y

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-

M

-

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-

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-

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A

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Y

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V

G
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G

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x
G

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G

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C

I

L

I

-

G

-

N

N

A

S

E

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K

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E

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F

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L

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T

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L

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x
M

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L

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H

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T

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A

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I

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I

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V

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N

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-

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N

D

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A

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E

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L

L

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D

F

F

E

-

A

G

C

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Q

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R

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E

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Y

L

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R

-

I

-

I

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-

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-

Y

-

A

-

I

-

K

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N

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M

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G

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L

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P

A

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I

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A

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I

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P

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I

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L

L

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x
H

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L

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M

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V

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W
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W

Q

E

E

D

L

R

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F

F

Y

D

K

K

A

R

N

Y

R

A

A

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H

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T

E

F

L

L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

-

E

I

I

N

F

P

P

D

N

F

M

Q

L

T

L

I

F

A

A

K

A

G

A

Y

C

H

G

I

I

P

P

S

A

L

A

K

R

V

V

S

T

E

K

R

K

Q

A

D

D

L

L

D

R

E

E

A

A

I

I

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Q

K

T

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M

V

L

E

D

A

T

D

P

G

G

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P

F

Y

L

L

E

D

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I

I

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K
x
H

E

Q

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E

N

H

V

V

F

L

P

P

M

M

V

I

P

P

A
x
S

G

G

A

G

S

T

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F

S

N

D

D

I

V

R

I

L

T

E

E

active site: Y33 (= Y32), G35 (= G34), G36 (= G35), A37 (= A36), S38 (≠ I37), E59 (= E59), T82 (= T82), F121 (= F121), Q122 (= Q122), E123 (= E123), K171 (= K171), M266 (= M266), V293 (= V293), V400 (= V399), G426 (= G425), M428 (= M427), D453 (= D452), N480 (= N479), H482 (≠ F481), L483 (= L482), M485 (= M484), V486 (= V485), W489 (= W488), H558 (≠ K549)
binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (= D291), R292 (= R292), V486 (= V485), W489 (= W488), S568 (≠ A559)
binding flavin-adenine dinucleotide: R161 (= R161), G222 (= G220), G223 (≠ Q221), G224 (= G222), T246 (= T246), L247 (= L247), M248 (≠ L248), L264 (= L264), G265 (= G265), M266 (= M266), H267 (= H267), G286 (= G286), V287 (≠ M287), R288 (= R288), D290 (= D290), V293 (= V293), D310 (≠ E310), I311 (= I311), D329 (≠ N329), V330 (= V330), Q404 (= Q403), M405 (= M404), G423 (= G422), G424 (= G423)
binding magnesium ion: D453 (= D452), N480 (= N479), H482 (≠ F481)

1ybhA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide chlorimuron ethyl (see paper)
44% identity, 98% coverage: 13:569/569 of query aligns to 14:578/582 of 1ybhA

query
sites
1ybhA

G

G

S

A

E

D

A

I

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L

I

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E

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A

L

L

L

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Y

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G

A
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A

I
x
S

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M

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E

I

I

Y

H

D

Q

A

A

L

L

H

-

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N

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E
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E

Q

Q

G

G

A

G

L

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H

F

A

A

E

E

G

G

Y

Y

A

A

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S

G

G

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K

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G

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C

C

F

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A

A

T
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T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

T

L

V

V

T

S

G

G

L

L

A

A

N

D

A

A

M

L

D

D

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S

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P

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L

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L

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G

E

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A

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I

I

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A

A

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R

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D

I

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K

D

D

A

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A

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I

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Y

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Y

W

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A

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Y

Y

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L

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P

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Y

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V

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G

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x
G

G
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G

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C

I

L

I

-

G

-

N

N

A

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E

S

K

D

E

E

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G

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T

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L

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x
M

G

G

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G

A

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Y

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P

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C

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H

D

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S

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M

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L

G

G

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M

H
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H

G

G

N

T

Y

V

G

Y

P

A

N

N

I

Y

K

A

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N

E

E

H

A

S

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D

L

L

I

L

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G

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x
V

R
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R

F

F

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D

R
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R

V
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V

T

T

G

G

D

K

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L

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A

Y

F

A

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S

N

R

A

A

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K

I

I

I

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M

I

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x
D

I
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I

D

D

P

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A

A

E

E

I

I

N

G

K

K

N

N

V

K

H

T

A

P

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H

V

V

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S

I

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L

C

G

G

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x
D

V
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V

K

K

K

L

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A

L

L

P

Q

L

G

L

M

T

N

E

-

K

K

I

V

V

L

K

E

N

N

D

R

H

A

S

E

D

E

W

L

L

K

K

L

E

D

F

F

E

-

A

G

C

V

Q

W

R

R

V

N

E

E

Y

L

N

N

R

-

I

-

I

V

E

Q

K

K

Q

Q

L

K

N

F

P

P

I

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S

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F

G

K

I

-

T

T

M

F

G

G

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E

V

A

V

I

A

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K

P

-

Q

-

Y

-

A

-

I

-

K

-

V

V

L

S

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Q

E

A

L

F

T

D

D

N

G

D

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A

A

I

I

M

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D

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V

G

G

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H

H

Q
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Q

M
|
M

M

W

G

A

A

A

R

Q

Y

F

F

Y

K

N

F

Y

K

K

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K

S

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R

R

S

Q

V

W

V

L

T

S

S

S

G
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G

L

L

G
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G

T

A

M
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M

G

G

F

F

G

G

L

L

P

P

A

A

M

I

G

G

A

A

K

S

L

V

G

A

A

N

P

P

D

D

R

A

Q

I

V

V

C

V

V

D

F

I

V

D

G

G

D
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D

G

G

G

S

L

F

Q

I

M

M

T

N

I

V

Q

Q

E

E

L

L

G

A

T

T

I

I

Y

R

Q

V

T

E

Q

N

I

L

P

P

V

V

K

K

I

V

V

L

L

L

N

N

N
|
N

N

Q

F
x
H

L
|
L

G

G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

E

D

L

R

F

F

F

Y

D

K

K

A

R

N

Y

R

A

A

S

H

T

T

E

F

L

L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

-

E

I

I

N

F

P

P

D

N

F

M

Q

L

T

L

I

F

A

A

K

A

G

A

Y

C

H

G

I

I

P

P

S

A

L

A

K

R

V

V

S

T

E

K

R

K

Q

A

D

D

L

L

D

R

E

E

A

A

I

I

Q

Q

K

T

M

M

V

L

E

D

A

T

D

P

G

G

P

P

F

Y

L

L

E

D

V

V

I

I

V

C

E

P

K
x
H

E

Q

G

E

N

H

V

V

F

L

P

P

M

M

V

I

P

P

A
x
S

G

G

A

G

S

T

V

F

S

N

D

D

I

V

R

I

L

T

E

E

active site: Y33 (= Y32), G35 (= G34), G36 (= G35), A37 (= A36), S38 (≠ I37), E59 (= E59), T82 (= T82), F121 (= F121), Q122 (= Q122), E123 (= E123), K171 (= K171), M266 (= M266), V293 (= V293), V400 (= V399), G426 (= G425), M428 (= M427), D453 (= D452), N480 (= N479), H482 (≠ F481), L483 (= L482), M485 (= M484), V486 (= V485), W489 (= W488), H558 (≠ K549)
binding 2-[[[[(4-chloro-6-methoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl]benzoic acid ethyl ester: M266 (= M266), D291 (= D291), R292 (= R292), M485 (= M484), W489 (= W488), S568 (≠ A559)
binding flavin-adenine dinucleotide: R161 (= R161), G223 (≠ Q221), G224 (= G222), T246 (= T246), L247 (= L247), M248 (≠ L248), L264 (= L264), M266 (= M266), H267 (= H267), G286 (= G286), V287 (≠ M287), R288 (= R288), D290 (= D290), V293 (= V293), D310 (≠ E310), I311 (= I311), D329 (≠ N329), V330 (= V330), Q404 (= Q403), M405 (= M404), G423 (= G422), G424 (= G423)
binding magnesium ion: D453 (= D452), N480 (= N479), H482 (≠ F481)

Query Sequence

>WP_044138357.1 NCBI__GCF_000220155.1:WP_044138357.1
MKEKTTKTTRMSGSEAVIQSLLREGTSIIFGYPGGAIMPIYDALHDHQKELHHVLTRHEQ
GALHAAEGYAKVTGKVGVCFATSGPGATNTVTGLANAMMDSTPVVLITGQVVSSLLGTDA
FQETDVVGVTQPVTKWNYQVRRAEDIPAAIARAFYIARSGRPGPVVIDITKDAQINQLDF
YYEPVKSIRSYTPEYPADLAQIEEAARLINLSKKPLALVGQGVIIGNAEKELLDFLEKSG
IPAAWTLLGLSAVPSDHPLNVGMLGMHGNYGPNIKTNEADLIVAIGMRFDDRVTGDLSRY
ATNAKIIHMEIDPAEINKNVHADVPILGNVKKTLPLLTEKIVKNDHSDWLKEFEACQRVE
YNRIIEKQLNPIEKGITMGEVVAKVSQAFDNDAIMVTDVGQHQMMGARYFKFKQSRSVVT
SGGLGTMGFGLPAAMGAKLGAPDRQVCVFVGDGGLQMTIQELGTIYQTQIPVKIVLLNNN
FLGMVRQWQELFFDKRYASTELINPDFQTIAKGYHIPSLKVSERQDLDEAIQKMVEADGP
FLLEVIVEKEGNVFPMVPAGASVSDIRLE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory