SitesBLAST

Q8ZKF6 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
27% identity, 93% coverage: 41:642/649 of query aligns to 24:635/652 of Q8ZKF6

query
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R194 (≠ K208) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 3-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 2-fold reduction for CoA.
T311 (≠ M321) binding CoA
N335 (≠ M343) binding CoA
A357 (≠ G365) mutation to V: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 3-fold reduction for CoA.
D517 (= D523) mutation to G: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 10-fold reduction for CoA.; mutation to P: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold reduction in the affinity for ATP and 4.5-fold reduction for CoA.
S523 (≠ G529) binding CoA
G524 (= G530) mutation to L: No acetyl-coenzyme A synthetase activity.; mutation to S: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. Almost the same affinity as the wild-type for ATP, but 9-fold reduction in the affinity for CoA.
R526 (= R532) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 4-fold reduction for CoA.
R584 (= R590) binding CoA; mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 7-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 8-fold reduction for CoA.
K609 (= K615) modified: N6-acetyllysine; by Pat; mutation to A: No acetyl-coenzyme A synthetase activity.

2p2fA Acetyl-coa synthetase, wild-type with acetate, amp, and coa bound (see paper)
28% identity, 92% coverage: 41:635/649 of query aligns to 19:624/637 of 2p2fA

query
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2p2fA

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T

P

S

K

S

T

T

S

L

A

A

N

D

active site: T259 (≠ S274), T411 (≠ S417), E412 (≠ L418), N516 (= N527), R521 (= R532), K604 (= K615)
binding adenosine monophosphate: G382 (= G394), E383 (≠ S395), P384 (= P396), T407 (≠ V413), W408 (= W414), W409 (≠ L415), Q410 (≠ I416), T411 (≠ S417), D495 (= D506), I507 (= I518), R510 (= R521), N516 (= N527), R521 (= R532)
binding coenzyme a: F158 (≠ S177), R186 (≠ Y205), W304 (= W319), T306 (≠ M321), P329 (= P342), A352 (≠ G365), A355 (≠ Y368), S518 (≠ G529), R579 (= R590), P584 (= P595)

5jrhA Crystal structure of salmonella enterica acetyl-coa synthetase (acs) in complex with camp and coenzyme a (see paper)
28% identity, 91% coverage: 41:633/649 of query aligns to 20:623/640 of 5jrhA

query
sites
5jrhA

Y

Y

Q

E

D

T

L

K

W

Y

E

K

W

Q

S

S

V

I

T

N

D

D

L

P

E

D

D

T

F

F

W

W

E

G

S

E

M

Q

W

G

Q

K

F

I

F

L

N

D

-

W

I

I

K

T

S

P

H

Y

E

Q

V

K

Y

V

L

K

E

N

V

T

I

S

S

F

R

A

P

P

K

-

S

-

G

G

M

N

I

V

G

S

T

I

K

K

W

W

F

Y

R

E

R

D

S

G

K

T

L

L

N

N

Y

L

A

A

E

A

H

N

I

C

F

L

R

D

-

R

-

H

-

L

N

Q

K

E

S

N

K

G

G

D

K

R

P

T

A

A

L

I

F
x
W

Q

E

S

G

E

D

T

D

R

T

G

S

L

Q

S

S

P

K

-

H

V

I

T

S

W

Y

E

R

R

E

L

L

E

H

A

R

D

D

V

V

A

C

A

R

V

F

A

A

A

N

W

T

L

L

R

L

Q

D

I

L

G

G

I

I

K

K

P

K

G

G

D

D

R

V

V

V

A

A

A

I

V

Y

F

M

P

P

N

M

C

V

P

P

E
|
E

A

A

I

A

I

V

A

A

F

M

L

L

A

A

T

C

N

A

A

R

V

I

G

G

A

A

V

V

W

H

S

S

S

V

C

I

S
x
F

P
x
G

D
x
G

F

F

G

S

V

P

A

E

G

A

I

V

A

A

D
x
G

R

R

F

I

Q

I

Q

D

I

S

E

S

P

S

K

R

V

L

L

V

F

I

L

T

C

A

D

D

G

E

Y

G

T

V

Y
x
R

N

A

G

G

K

R

S

S

Y

I

P

P

S

-

T

-

L

L

Q

K

L

K

A

N

L

V

A

D

E

D

A

A

L

L

-

K

-

N

P

P

S

N

V

V

N

T

D

S

V

V

-

E

-

H

-

V

-

I

V

V

F

L

V

K

K

R

T

T

G

G

-

S

D

D

F

I

G

D

L

W

Q

Q

P

E

E

G

R

R

T

D

T

L

A

W

W

W

E

R

D

D

I

L

L

I

H

E

F

K

P

A

H

S

H

P

G

E

L

H

E

Q

F

P

E

E

P

A

V

M

P

N

F

A

D

E

H

D

P

P

I

L

W

F

V

I

L

L

Y

Y

S
x
T

S

S

G

G

T

S

T

T

G

G

K
|
K

P
|
P

K

K

A

G

I

V

T

L

H

H

G

T

V

T

G

G

C

Y

L

L

L

V

E

Y

H

A

Y

A

K

T

A

T

L

F

A

K

L

Y

H

V

Q

F

N

D

V

Y

K

H

P

P

G

G

D

D

R

I

Y

Y

F

W

W

C

Y

T

S

A

T

D

T

V

G

G

W

W

M

V

M

T

W

G

N

H

Y

S

A

Y

L

L

-

L

-

Y

G

G

S

P

M

L

L

A

C

C

G

G

S

A

T

T

L

T

V

L

I

M

Y

F

D

E

G

G

A

V

P

P

M

N

Y

W

P

P

S

T

S

P

Q

A

T

R

L

M

W

C

A

Q

L

V

A

V

E

D

Q

K

A

H

K

Q

I

V

T

N

H

I

F

L

G

Y

S

T

G

A

A

P

A

T

Y

A

F

I

I

R

A

A

C

L

M

M

K

A

A

E

G

G

-

D

V

K

S

A

I

I

A

E

N

G

D

T

Q

D

L

R

K

S

S

S

L

L

E

R

T

I

I

L

G

G

S

S

T

V

G
|
G

S
x
E

P
|
P

L

I

T

N

A

P

D

E

A

A

Y

W

E

E

W

W

I

Y

Y

W

K

K

F

K

V

I

K

G

K

K

-

E

-

K

-

C

-

P

-

V

D

D

V
x
T

W
|
W

L
x
W

I
x
Q

S
x
T

L
x
E

S

T

G

G

T

F

D

M

V

I

C

-

S

-

A

-

F

-

V

-

G

-

S

T

P

P

L

L

-

P

-

G

-

A

L

I

P

E

V

L

Y

K

A

A

G

G

E

S

I

A

Q

T

C

R

R

P

M

F

L

F

G

G

C

V

K

Q

I

P

E

A

A

L

Y

V

D

D

E

N

N

E

G

G

Q

H

P

P

Q

Q

L

E

N

G

Q

A

L

T

-

E

G

G

E

N

M

L

V

V

I

I

T

T

Q

D

P

S

L

W

P

P

S

G

M

Q

P

A

I

R

Y

T

F

L

W

F

N

G

D

D

P

-

N

-

Q

H

E

E

R

R

Y

F

L

E

S

Q

S

T

Y

Y

F

F

E

S

T

T

Y

F

P

K

N

N

I

M

W

Y

R

F

H

S

G

G

D
|
D

W

G

I

A

K

R

I

R

N

D

D

E

R

D

G

G

G

Y

V

Y

V

W

I
|
I

Q

T

G

G

R

R

S

V

D

D

T

D

T

V

L

L

N
|
N

R

V

G
x
S

G

G

V

H

R
|
R

I

L

G

G

T

T

A

A

E

E

I

I

Y

E

S

S

A

A

V

L

E
x
V

S

A

L
x
H

P

P

P

K

V
x
I

K

A

D

E

S

A

L

A

V

V

Y

G

L

I

E

P

K

H

S

A

D

I

G

K

E

G

G

Q

T

A

I

I

S

Y

L

A

F

Y

V

V

V

T

L

L

K

N

K

H

G

G

H

E

Q

E

L

P

T

S

E

P

E

E

L

L

K

Y

K

A

T

E

I

V

K

R

N

N

T

W

L

V

R
|
R

Q

K

Q

E

Y

I

S

G

P
|
P

R

L

H

A

V

T

P

P

D

D

T

V

I

L

E

H

E

W

V

T

A

D

D

S

I

L

P

P

Y

K

T

T

I

R

S

S

G

G

K
|
K

K

I

M

M

E

R

A

R

P

I

V

L

K

R

K

K

I

I

L

A

M

A

G

G

Q

D

D

L

P

G

K

D

T

T

S

S

active site: T260 (≠ S274), T412 (≠ S417), E413 (≠ L418), N517 (= N527), R522 (= R532), K605 (= K615)
binding (r,r)-2,3-butanediol: W93 (≠ F112), E140 (= E158), G169 (≠ D187), K266 (= K280), P267 (= P281)
binding adenosine-3',5'-cyclic-monophosphate: G383 (= G394), E384 (≠ S395), P385 (= P396), T408 (≠ V413), W409 (= W414), W410 (≠ L415), Q411 (≠ I416), T412 (≠ S417), D496 (= D506), I508 (= I518), N517 (= N527), R522 (= R532)
binding coenzyme a: F159 (≠ S177), G160 (≠ P178), G161 (≠ D179), R187 (≠ Y205), S519 (≠ G529), R580 (= R590), P585 (= P595)
binding magnesium ion: V533 (≠ E543), H535 (≠ L545), I538 (≠ V548)

2p20A Acetyl-coa synthetase, r584a mutation (see paper)
27% identity, 93% coverage: 41:642/649 of query aligns to 20:629/641 of 2p20A

query
sites
2p20A

Y

Y

Q

E

D

T

L

K

W

Y

E

K

W

Q

S

S

V

I

T

N

D

D

L

P

E

D

D

T

F

F

W

W

E

G

S

E

M

Q

W

G

Q

K

F

I

F

L

N

D

-

W

I

I

K

T

S

P

H

Y

E

Q

V

K

Y

V

L

K

E

N

V

T

I

S

S

F

R

A

P

P

K

-

S

-

G

G

M

N

I

V

G

S

T

I

K

K

W

W

F

Y

R

E

R

D

S

G

K

T

L

L

N

N

Y

L

A

A

E

A

H

N

I

C

F

L

R

D

-

R

-

H

-

L

N

Q

K

E

S

N

K

G

G

D

K

R

P

T

A

A

L

I

F

W

Q

E

S

G

E

D

T

D

R

T

G

S

L

Q

S

S

P

K

-

H

V

I

T

S

W

Y

E

R

R

E

L

L

E

H

A

R

D

D

V

V

A

C

A

R

V

F

A

A

A

N

W

T

L

L

R

L

Q

D

I

L

G

G

I

I

K

K

P

K

G

G

D

D

R

V

V

V

A

A

A

I

V

Y

F

M

P

P

N

M

C

V

P

P

E

E

A

A

I

A

I

V

A

A

F

M

L

L

A

A

T

C

N

A

A

R

V

I

G

G

A

A

V

V

W

H

S

S

S

V

C

I

S

F

P

G

D

G

F

F

G

S

V

P

A

E

G

A

I

V

A

A

D

G

R

R

F

I

Q

I

Q

D

I

S

E

S

P

S

K

R

V

L

L

V

F

I

L

T

C

A

D

D

G

E

Y

G

T

V

Y

R

N

A

G

G

K

R

S

S

Y

I

P

P

S

-

T

-

L

L

Q

K

L

K

A

N

L

V

A

D

E

D

A

A

L

L

-

K

-

N

P

P

S

N

V

V

N

T

D

S

V

V

-

E

-

H

-

V

-

I

V

V

F

L

V

K

K

R

T

T

G

G

-

S

D

D

F

I

G

D

L

W

Q

Q

P

E

E

G

R

R

T

D

T

L

A

W

W

W

E

R

D

D

I

L

L

I

H

E

F

K

P

A

H

S

H

P

G

E

L

H

E

Q

F

P

E

E

P

A

V

M

P

N

F

A

D

E

H

D

P

P

I

L

W

F

V

I

L

L

Y

Y

S
x
T

S

S

G

G

T

S

T

T

G

G

K

K

P

P

K

K

A

G

I

V

T

L

H

H

G

T

V

T

G

G

C

Y

L

L

L

V

E

Y

H

A

Y

A

K

T

A

T

L

F

A

K

L

Y

H

V

Q

F

N

D

V

Y

K

H

P

P

G

G

D

D

R

I

Y

Y

F

W

W

C

Y

T

S

A

T

D

T

V

G

G

W

W

M

V

M

T

W

G

N

H

Y

S

A

Y

L

L

-

L

-

Y

G

G

S

P

M

L

L

A

C

C

G

G

S

A

T

T

L

T

V

L

I

M

Y

F

D

E

G

G

A

V

P

P

M

N

Y

W

P

P

S

T

S

P

Q

A

T

R

L

M

W

C

A

Q

L

V

A

V

E

D

Q

K

A

H

K

Q

I

V

T

N

H

I

F

L

G

Y

S

T

G

A

A

P

A

T

Y

A

F

I

I

R

A

A

C

L

M

M

K

A

A

E

G

G

-

D

V

K

S

A

I

I

A

E

N

G

D

T

Q

D

L

R

K

S

S

S

L

L

E

R

T

I

I

L

G

G

S

S

T

V

G
|
G

S
x
E

P
|
P

L

I

T

N

A

P

D

E

A

A

Y

W

E

E

W

W

I

Y

Y

W

K

K

F

K

V

I

K

G

K

K

-

E

-

K

-

C

-

P

-

V

D

D

V
x
T

W
|
W

L
x
W

I
x
Q

S
x
T

L
x
E

S

T

G

G

T

F

D

M

V

I

C

-

S

-

A

-

F

-

V

-

G

-

S

T

P

P

L

L

-

P

-

G

-

A

L

I

P

E

V

L

Y

K

A

A

G

G

E

S

I

A

Q

T

C

R

R

P

M

F

L

F

G

G

C

V

K

Q

I

P

E

A

A

L

Y

V

D

D

E

N

N

E

G

G

Q

H

P

P

Q

Q

L

E

N

G

Q

A

L

T

-

E

G

G

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N

M

L

V

V

I

I

T

T

Q

D

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S

L

W

P

P

S

G

M

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A

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Y

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F

L

W

F

N

G

D

D

P

-

N

-

Q

H

E

E

R

R

Y

F

L

E

S

Q

S

T

Y

Y

F

F

E

S

T

T

Y

F

P

K

N

N

I

M

W

Y

R

F

H

S

G

G

D
|
D

W

G

I

A

K

R

I

R

N

D

D

E

R

D

G

G

G

Y

V

Y

V

W

I
|
I

Q

T

G

G

R
|
R

S

V

D

D

T

D

T

V

L

L

N
|
N

R

V

G

S

G

G

V

H

R
|
R

I

L

G

G

T

T

A

A

E

E

I

I

Y

E

S

S

A

A

V

L

E

V

S

A

L

H

P

P

P

K

V

I

K

A

D

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S

A

L

A

V

V

Y

G

L

I

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P

K

H

S

A

D

I

G

K

E

G

G

Q

T

A

I

I

S

Y

L

A

F

Y

V

V

V

T

L

L

K

N

K

H

G

G

H

E

Q

E

L

P

T

S

E

P

E

E

L

L

K

Y

K

A

T

E

I

V

K

R

N

N

T

W

L

V

R

A

Q

K

Q

E

Y

I

S

G

P

P

R

L

H

A

V

T

P

P

D

D

T

V

I

L

E

H

E

W

V

T

A

D

D

S

I

L

P

P

Y

K

T

T

I

R

S

S

G

G

K
|
K

K

I

M

M

E

R

A

R

P

I

V

L

K

R

K

K

I

I

L

A

M

A

G

G

Q

D

D

-

P

-

K

-

T

T

S

S

A

N

N

D

L

T

A

S

T

T

M

L

R

A

N

D

P

P

active site: T260 (≠ S274), T412 (≠ S417), E413 (≠ L418), N517 (= N527), R522 (= R532), K605 (= K615)
binding adenosine-5'-monophosphate-propyl ester: G383 (= G394), E384 (≠ S395), P385 (= P396), T408 (≠ V413), W409 (= W414), W410 (≠ L415), Q411 (≠ I416), T412 (≠ S417), D496 (= D506), I508 (= I518), R511 (= R521), R522 (= R532)

P27550 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Escherichia coli (strain K12) (see paper)
27% identity, 93% coverage: 39:642/649 of query aligns to 22:635/652 of P27550

query
sites
P27550

R

Q

S

Q

Y

Y

Q

E

D

A

L

M

W

Y

E

Q

W

Q

S

S

V

I

T

N

D

V

L

P

E

D

D

T

F

F

W

W

E

G

S

E

M

Q

W

G

Q

K

F

I

F

L

N

D

-

W

I

I

K

K

S

P

H

Y

E

Q

V

K

Y

V

L

K

E

N

V

T

I

S

S

F

R

A

P

P

K

-

S

-

G

G

M

N

I

V

G

S

T

I

K

K

W

W

F

Y

R

E

R

D

S

G

K

T

L

L

N

N

Y

L

A

A

E

A

H

N

I

C

F

L

R

D

-

R

-

H

-

L

N

Q

K

E

S

N

K

G

G

D

K

R

P

T

A

A

L

I

F

W

Q

E

S

G

E

D

T

D

R

A

G

S

L

Q

S

S

P

K

-

H

V

I

T

S

W

Y

E

K

R

E

L

L

E

H

A

R

D

D

V

V

A

C

A

R

V

F

A

A

A

N

W

T

L

L

R

L

Q

E

I

L

G

G

I

I

K

K

P

K

G

G

D

D

R

V

V

V

A

A

A

I

V

Y

F

M

P

P

N

M

C

V

P

P

E

E

A

A

I

A

I

V

A

A

F

M

L

L

A

A

T

C

N

A

A

R

V

I

G

G

A

A

V

V

W

H

S

S

S

V

C

I

S

F

P

G

D

G

F

F

G

S

V

P

A

E

G

A

I

V

A

A

D

G

R

R

F

I

Q

I

Q

D

I

S

E

N

P

S

K

R

V

L

L

V

F

I

L

T

C

S

D

D

G

E

Y

G

T

V

Y

R

N

A

G

G

K

R

S

S

Y

I

P

P

S

-

T

-

L

L

Q

K

L

K

A

N

L

V

A

D

E

D

A

A

L

L

P

K

-

N

-

P

-

N

-

V

-

T

S

S

V

V

N

E

D

H

V

V

F

V

V

L

K

K

T

R

-

T

-

G

G

G

D

K

F

I

G

D

L

W

Q

Q

P

E

E

G

R

R

T

D

T

L

A

W

W

W

E

H

D

D

I

L

L

V

H

E

F

Q

P

A

H

S

H

D

G

Q

L

H

E

Q

F

A

E

E

P

E

V

M

P

N

F

A

D

E

H

D

P

P

I

L

W

F

V

I

L

L

Y

Y

S

T

S

S

G

G

T

S

T

T

G

G

K

K

P

P

K

K

A

G

I

V

T

L

H

H

G

T

V

T

G

G

C

Y

L

L

L

V

E

-

H

-

Y

Y

K

A

A

A

L

L

A

T

L

F

H

K

Q

Y

-

V

-

F

N

D

V

Y

K

H

P

P

G

G

D

D

R

I

Y

Y

F

W

W

C

Y

T

S

A

T

D

T

V

G

G

W

W

M

V

M

T

W

G

N

H

Y

S

A

Y

L

L

-

L

-

Y

G

G

S

P

M

L

L

A

C

C

G

G

S

A

T

T

L

T

V

L

I

M

Y

F

D

E

G

G

A

V

P

P

M

N

Y

W

P

P

S

T

S

P

Q

A

T

R

L

M

W

A

A

Q

L

V

A

V

E

D

Q

K

A

H

K

Q

I

V

T

N

H

I

F

L

G

Y

S

T

G

A

A

P

A

T

Y

A

F

I

I

R

A

A

C

L

M

M

K

A

A

E

G

G

-

D

V

K

S

A

I

I

A

E

N

G

D

T

Q

D

L

R

K

S

S

S

L

L

E

R

T

I

I

L

G

G

S

S

T

V

G

G

S

E

P

P

L

I

T

N

A

P

D

E

A

A

Y

W

E

E

W

W

I

Y

Y

W

K

K

F

K

V

I

K

G

K

N

-

E

-

K

-

C

-

P

-

V

D

D

V

T

W

W

L

W

I

Q

S

T

L

E

S

T

G

G

T

F

D

-

V

M

C

I

S

T

A

P

F

L

V

P

G

G

G

A

S

T

P

E

L

L

L

-

P

-

V

-

Y

K

A

A

G

G

E

S

I

A

Q

T

C

R

R

P

M

F

L

F

G

G

C

V

K

Q

I

P

E

A

A

L

Y

V

D

D

E

N

N

E

G

G

Q

N

P

P

-

L

Q

E

L

G

N

A

Q

T

L

E

G

G

E

S

M

L

V

V

I

I

T

T

Q

D

P

S

L

W

P

P

S

G

M

Q

P

A

I

R

Y

T

F

L

W

F

N

G

D

D

P

-

N

-

Q

H

E

E

R

R

Y

F

L

E

S

Q

S

T

Y

Y

F

F

E

S

T

T

Y

F

P

K

N

N

I

M

W

Y

R

F

H

S

G

G

D

D

W

G

I

A

K

R

I

R

N

D

D

E

R

D

G

G

G

Y

V

Y

V

W

I

I

Q

T

G

G

R

R

S

V

D

D

T

D

T

V

L

L

N

N

R

V

G

S

G

G

V

H

R

R

I

L

G

G

T

T

A

A

E

E

I

I

Y

E

S

S

A

A

V

L

E

V

S

A

L

H

P

P

P

K

V

I

K

A

D

E

S

A

L

A

V

V

Y

G

L

I

E

P

K

H

S

N

D

I

G

K

E

G

G

Q

T

A

I

I

S

Y

L

A

F

Y

V

V

V

T

L

L

K

N

K

H

G

G

H

E

Q

E

L

P

T

S

E

P

E

E

L

L

K

Y

K

A

T

E

I

V

K

R

N

N

T

W

L

V

R

R

Q

K

Q

E

Y

I

S

G

P

P

R

L

H

A

V

T

P

P

D

D

T

V

I

L

E

H

E

W

V

T

A

D

D

S

I

L

P

P

Y

K

T

T

I

R

S

S

G

G

K
|
K

K

I

M

M

E

R

A

R

P

I

V

L

K

R

K

K

I

I

L

A

M

A

G

G

Q

-

D

D

P

T

K

S

T

N

S

L

A

G

N

D

L

T

A

S

T

T

M

L

R

A

N

D

P

P

K609 (= K615) modified: N6-acetyllysine; by autocatalysis

1pg3A Acetyl coa synthetase, acetylated on lys609 (see paper)
27% identity, 92% coverage: 41:636/649 of query aligns to 20:628/634 of 1pg3A

query
sites
1pg3A

Y

Y

Q

E

D

T

L

K

W

Y

E

K

W

Q

S

S

V

I

T

N

D

D

L

P

E

D

D

T

F

F

W

W

E

G

S

E

M

Q

W

G

Q

K

F

I

F

L

N

D

-

W

I

I

K

T

S

P

H

Y

E

Q

V

K

Y

V

L

K

E

N

V

T

I

S

S

F

R

A

P

P

K

-

S

-

G

G

M

N

I

V

G

S

T

I

K

K

W

W

F

Y

R

E

R

D

S

G

K

T

L

L

N

N

Y

L

A

A

E

A

H

N

I

C

F

L

R

D

-

R

-

H

-

L

N

Q

K

E

S

N

K

G

G

D

K

R

P

T

A

A

L

I

F

W

Q

E

S

G

E

D

T

D

R

T

G

S

L

Q

S

S

P

K

-

H

V

I

T

S

W

Y

E

R

R

E

L

L

E

H

A

R

D

D

V

V

A

C

A

R

V

F

A

A

A

N

W

T

L

L

R

L

Q

D

I

L

G

G

I

I

K

K

P

K

G

G

D

D

R

V

V

V

A

A

A

I

V

Y

F

M

P

P

N

M

C

V

P

P

E

E

A

A

I

A

I

V

A

A

F

M

L

L

A

A

T

C

N

A

A

R

V

I

G

G

A

A

V

V

W

H

S

S

S

V

C

I

S
x
F

P
x
G

D

G

F

F

G

S

V

P

A

E

G

A

I

V

A

A

D

G

R

C

F

I

Q

I

Q

D

I

S

E

S

P

S

K

R

V

L

L

V

F

I

L

T

C

A

D

D

G

E

Y

G

T

V

Y
x
R

N

A

G

G

K
x
R

S

S

Y

I

P

P

S

-

T

-

L

L

Q

K

L

K

A

N

L

V

A

D

E

D

A

A

L

L

-

K

-

N

P

P

S

N

V

V

N

T

D

S

V

V

-

E

-

H

-

V

-

I

V

V

F

L

V

K

K

R

T

T

G

G

-

S

D

D

F

I

G

D

L

W

Q

Q

P

E

E

G

R

R

T

D

T

L

A

W

W

W

E

R

D

D

I

L

L

I

H

E

F

K

P

A

H

S

H

P

G

E

L

H

E

Q

F

P

E

E

P

A

V

M

P

N

F

A

D

E

H

D

P

P

I

L

W

F

V

I

L

L

Y

Y

S
x
T

S

S

G

G

T

S

T

T

G

G

K

K

P

P

K

K

A

G

I

V

T

L

H

H

G

T

V

T

G

G

C

Y

L

L

L

V

E

Y

H

A

Y

A

K

T

A

T

L

F

A

K

L

Y

H

V

Q

F

N

D

V

Y

K

H

P

P

G

G

D

D

R

I

Y

Y

F

W

W

C

Y

T

S
x
A

T

D

T

V

G

G

W

W

M

V

M
x
T

W

G

N

H

Y

S

A

Y

L

L

-

L

-

Y

G

G

S

P

M

L

L

A

C

C

G

G

S

A

T

T

L

T

V

L

I

M

Y

F

D

E

G

G

A

V

P
|
P

M

N

Y

W

P

P

S

T

S

P

Q

A

T

R

L

M

W

C

A

Q

L

V

A

V

E

D

Q

K

A

H

K

Q

I

V

T

N

H

I

F

L

G

Y

S

T

G

A

A

P

A

T

Y
x
A

F

I

I

R

A

A

C

L

M

M

K

A

A

E

G

G

-

D

V

K

S

A

I

I

A

E

N

G

D

T

Q

D

L

R

K

S

S

S

L

L

E

R

T

I

I

L

G

G

S

S

T

V

G
|
G

S
x
E

P
|
P

L

I

T

N

A

P

D

E

A

A

Y

W

E

E

W

W

I

Y

Y

W

K

K

F

K

V

I

K

G

K

K

-

E

-

K

-

C

-

P

-

V

D

D

V
x
T

W
|
W

L
x
W

I
x
Q

S
x
T

L
x
E

S

T

G

G

T

F

D

M

V

I

C

-

S

-

A

-

F

-

V

-

G

-

S

T

P

P

L

L

-

P

-

G

-

A

L

I

P

E

V

L

Y

K

A

A

G

G

E

S

I

A

Q

T

C

R

R

P

M

F

L

F

G

G

C

V

K

Q

I

P

E

A

A

L

Y

V

D

D

E

N

N

E

G

G

Q

H

P

P

Q

Q

L

E

N

G

Q

A

L

T

-

E

G

G

E

N

M

L

V

V

I

I

T

T

Q

D

P

S

L

W

P

P

S

G

M

Q

P

A

I

R

Y

T

F

L

W

F

N

G

D

D

P

-

N

-

Q

H

E

E

R

R

Y

F

L

E

S

Q

S

T

Y

Y

F

F

E

S

T

T

Y

F

P

K

N

N

I

M

W

Y

R

F

H

S

G

G

D
|
D

W

G

I

A

K

R

I

R

N

D

D

E

R

D

G

G

G

Y

V

Y

V

W

I

I

Q

T

G

G

R
|
R

S

V

D

D

T

D

T

V

L

L

N
|
N

R

V

G
x
S

G

G

V

H

R
|
R

I

L

G

G

T

T

A

A

E

E

I

I

Y

E

S

S

A

A

V

L

E
x
V

S

A

L
x
H

P

P

P

K

V
x
I

K

A

D

E

S

A

L

A

V

V

Y

G

L

I

E

P

K

H

S

A

D

I

G

K

E

G

G

Q

T

A

I

I

S

Y

L

A

F

Y

V

V

V

T

L

L

K

N

K

H

G

G

H

E

Q

E

L

P

T

S

E

P

E

E

L

L

K

Y

K

A

T

E

I

V

K

R

N

N

T

W

L

V

R
|
R

Q

K

Q

E

Y

I

S

G

P
|
P

R

L

H

A

V

T

P

P

D

D

T

V

I

L

E

H

E

W

V

T

A

D

D

S

I

L

P

P

Y

K

T

T

I

R

S

S

G

G

K
|
K

K

I

M

M

E

R

A

R

P

I

V

L

K

R

K

K

I

I

L

A

M

A

G

G

Q

D

-

L

-

A

D

D

P

P

K

G

T

V

S

V

A

E

N

K

L

L

active site: T260 (≠ S274), T412 (≠ S417), E413 (≠ L418), N517 (= N527), R522 (= R532), K605 (= K615)
binding coenzyme a: F159 (≠ S177), G160 (≠ P178), R187 (≠ Y205), R190 (≠ K208), A301 (≠ S315), T307 (≠ M321), P330 (= P342), A356 (≠ Y368), S519 (≠ G529), R580 (= R590), P585 (= P595)
binding magnesium ion: V533 (≠ E543), H535 (≠ L545), I538 (≠ V548)
binding adenosine-5'-monophosphate-propyl ester: G383 (= G394), E384 (≠ S395), P385 (= P396), T408 (≠ V413), W409 (= W414), W410 (≠ L415), Q411 (≠ I416), T412 (≠ S417), D496 (= D506), R511 (= R521), R522 (= R532)

Q89WV5 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) (see paper)
28% identity, 94% coverage: 41:649/649 of query aligns to 23:639/648 of Q89WV5

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