SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_044414029.1 NCBI__GCF_000934765.1:WP_044414029.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4rjiC Acetolactate synthase from bacillus subtilis bound to thdp - crystal form i (see paper)
38% identity, 99% coverage: 2:540/543 of query aligns to 3:553/555 of 4rjiC

query
sites
4rjiC

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binding magnesium ion: D438 (= D430), D465 (= D457), T467 (≠ E459)
binding thiamine diphosphate: P24 (= P23), E48 (= E47), P74 (= P73), S387 (≠ A379), H388 (= H380), Q411 (≠ A403), G437 (= G429), D438 (= D430), G439 (≠ A431), G440 (= G432), T467 (≠ E459), Y468 (= Y460), D469 (≠ G461), M470 (≠ L462), V471 (≠ I463), Y534 (= Y526)

4rjkG Acetolactate synthase from bacillus subtilis bound to lthdp - crystal form ii (see paper)
38% identity, 99% coverage: 2:540/543 of query aligns to 2:552/553 of 4rjkG

query
sites
4rjkG

K

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binding magnesium ion: D437 (= D430), D464 (= D457), T466 (≠ E459)
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-carboxy-1-hydroxyethyl)-5-(2-{[hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: E47 (= E47), Q110 (= Q110)
binding thiamine diphosphate: I384 (≠ V377), G385 (= G378), S386 (≠ A379), H387 (= H380), Q410 (≠ A403), L412 (≠ M405), G436 (= G429), D437 (= D430), G438 (≠ A431), G439 (= G432), T466 (≠ E459), Y467 (= Y460), D468 (≠ G461), M469 (≠ L462), V470 (≠ I463), Y533 (= Y526)

4rjkF Acetolactate synthase from bacillus subtilis bound to lthdp - crystal form ii (see paper)
38% identity, 99% coverage: 2:540/543 of query aligns to 2:552/552 of 4rjkF

query
sites
4rjkF

K

R

A

G

A

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E

E

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Q

E

I

V

L

Q

S

D

P

V

D

K

I

N

I

I

P

P

E

E

V

A

V

V

R

T

K

N

A

A

F

F

K

R

V

I

A

A

Q

S

M

A

E

G

K

Q

P

A

G

G

C

A

S

A

F

F

I

V

D

S

F

F

P

P

E

Q

N

D

I

V

A

V

E

N

M

E

Q

V

I

T

D

N

K

T

K

K

P

N

L

V

K

R

R

A

-

V

Q

A

S

A

P

P

S

K

L

L

P

G

A

P

P

A

N

A

T

D

E

D

K

A

L

I

R

S

Q

A

A

A

A

I

D

A

I

K

I

I

S

Q

Q

T

A

A

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K

H

L

P

P

V

V

I

V

I

L

A

V

G

G

N

M

G

K

V

G

I

G

R

R

A

P

R

E

A

A

S

I

E

K

A

A

L

V

I

R

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K

F

L

A

L

A

K

K

K

L

V

N

Q

I

L

P

P

V

F

A

V

T

E

T

T

F

Y

M

Q

A

A

K

A

G

G

V

T

I

L

P

S

C

R

R

D

-

L

H

E

P

D

L

Q

S

Y

I

F

G

G

T

R

I

I

G

G

L

L

Q

F

A

R

H

N

D

Q

Y

P

I

G

A

D

C

L

G

L

V

L

D

E

R

Q

A

A

D

D

V

V

I

L

C

T

V

I

G

G

Y

Y

D

D

I

P

V

I

E

E

Y

Y

H

D

P

P

Y

K

L

F

W

W

N

N

K

I

N

N

P

G

N

D

C

R

K

T

I

I

H

H

I

L

D

D

T

E

Q

I

P

I

A

A

E

D

V

I

D

D

E

H

Y

A

Y

Y

I

Q

L

P

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D

A

L

G

E

V

L

L

I

G

G

D

D

I

I

G

P

R

S

S

T

L

I

E

N

G

H

I

I

A

E

A

H

L

D

A

A

H

V

R

K

-

V

-

E

-

F

-

A

-

E

Q

R

E

E

D

Q

N

K

G

I

I

L

G

S

N

D

L

L

K

K

Q

Q

I

Y

I

M

E

-

Y

H

E

E

F

G

H

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Q

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V

-

P

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A

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D

Y

W

D

K

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S

D

D

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A

A

F

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P

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-

Q

-

K

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I

I

I

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H

K

D

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L

L

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N

A

A

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V

A

D

D

D

E

H

D

V

I

T

V

V

V

T

C

C

D

D

V

I

G
|
G

A
x
S

H
|
H

K

A

M

I

W

W

M

M

A

S

R

R

M

Y

Y

F

N

R

A

S

Q

Y

A

E

P

P

N

L

T

T

C

L

I

M

I

I

S

S

N

N

G

G

F

M

A
x
Q

S

T

M
x
L

G

G

I

V

A

A

L

L

P

P

G

W

A

A

M

I

A

G

A

A

K

S

L

L

A

V

Q

K

P

P

D

G

Q

E

V

K

A

V

V

V

A

S

V

V

T

S

G
|
G

D
|
D

A
x
G

G
|
G

F

F

L

L

M

F

N

S

C

A

Q

M

E

E

I

L

E

E

T

T

A

A

V

V

R

R

L

L

N

K

L

A

A

P

L

I

I

V

V

H

L

I

V

V

W

W

N

N

D
|
D

Q

S

E
x
T

Y
|
Y

G
x
D

L
x
M

I
x
V

K

A

W

F

K

Q

Q

Q

M

L

N

K

Q

K

F

Y

G

N

R

R

E

T

S

S

H

A

I

V

Q

D

F

F

A

G

N

N

P

I

D

D

F

I

I

V

K

K

F

Y

A

A

E

E

S

S

F

F

G

G

A

A

K

T

G

G

Y

L

R

R

I

V

N

E

H

S

T

P

D

D

E

Q

L

L

L

A

P

D

T

V

L

L

Q

R

Q

Q

A

G

I

M

A

N

D

A

N

E

C

G

V

P

V

V

I

I

D

D

C

V

P

P

V

V

D

D

Y
|
Y

S

S

E

D

N

N

L

I

-

N

-

L

-

A

-

S

-

D

K

K

L

L

T

P

E

K

Q

E

L

F

G

G

Q

E

M

L

V

M

binding magnesium ion: D437 (= D430), D464 (= D457), T466 (≠ E459)
binding pyruvic acid: A25 (≠ E25), K26 (≠ E26)
binding thiamine diphosphate: P23 (= P23), E47 (= E47), P73 (= P73), G385 (= G378), S386 (≠ A379), H387 (= H380), Q410 (≠ A403), L412 (≠ M405), G436 (= G429), D437 (= D430), G438 (≠ A431), G439 (= G432), T466 (≠ E459), Y467 (= Y460), D468 (≠ G461), M469 (≠ L462), V470 (≠ I463), Y533 (= Y526)

5dx6B Acetolactate synthase from klebsiella pneumoniae soaked with beta- fluoropyruvate
36% identity, 98% coverage: 3:536/543 of query aligns to 19:551/557 of 5dx6B

query
sites
5dx6B

A

G

A

A

E

D

L

L

M

V

V

V

R

S

C

Q

L

L

E

E

N

A

E

Q

E

G

V

V

E

R

Y

Q

I

V

F

F

G

G

I
|
I

P

P

G
|
G

E
x
A

E
x
K

N
x
I

L

D

D

K

L

V

M

F

D

D

A

S

L

L

D

D

S

S

P

S

I

I

K

R

F

I

I

I

L

P

T

V

R

R

H

H

E
|
E

Q

A

G

N

G

A

A

A

F

F

M

M

A

A

D

A

V

A

Y

V

G

G

R

R

L

I

T

T

G

G

K

K

A

A

G

G

V

V

C

A

L

L

S

V

T
|
T

L

S

G

G

P

P

G

G

A

C

T

S

N

N

L

L

V

I

T

T

G

G

V

M

A

A

D

T

A

A

N

N

M

S

D

E

R

G

A

D

P

P

V

V

A

A

I

L

A

G

G

G

Q

A

A

V

A

K

T

R

T

A

R

D

L

K

H

A

K

K

E

Q

S

V

H
|
H

Q
|
Q

V
x
S

L

M

D

D

L

T

V

V

N

A

L

M

F

F

Q
x
S

P

P

I

V

T
|
T

K

K

Y

Y

S

A

T

I

Q

E

I

V

L

T

S

A

P

P

D

D

I

A

I

L

P

A

E

E

V

V

R

S

K

N

A

A

F

F

K

R

V

A

A

A

Q

E

M

Q

E

G

K

R

P

P

G

G

C

S

S

A

F

F

I

V

D

S

F

L

P

P

E
x
Q

N

D

I

V

A

V

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D

M

G

Q

P

I

V

D

S

K

G

K

K

P

V

L

L

K

P

R

A

Q

S

-

G

S

A

P

P

S

Q

L

M

P

G

A

A

P

A

N

P

T

D

E

D

K

A

L

I

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D

Q

Q

A

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A

A

D

K

I

L

I

I

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A

Q

Q

A

A

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K

H

N

P

P

V

I

I

F

I

L

A

L

G

G

N

L

G

M

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A

I

S

R

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A

P

R

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A

N

S

S

E

K

A

A

L

L

I

R

E

R

F

L

A

L

A

E

K

T

L

S

N

H

I

I

P

P

V

V

A

T

T

S

T

T

F

Y

M

Q

A

A

K

A

G

G

V

A

I

V

P

N

-

Q

-

D

-

N

-

F

C

S

R

R

H

F

P

A

L
x
G

S
x
R

I

V

G

G

T
x
L

I

F

G

N

L

N

Q
|
Q

A

A

H

G

D

D

Y

R

I

L

A

-

C

-

G

-

V

L

D

Q

R

L

A

A

D

D

V

L

V

V

I

I

C

C

V

I

G

G

Y

Y

D

S

I

P

V

V

E

E

Y

Y

H
x
E

P

P

Y

A

L

M

W

W

N

N

K

S

N

G

P

-

N

N

C

A

K

T

I

L

I

V

H

H

I

I

D

D

T

V

Q

L

P

P

A

A

E

Y

V

E

D

E

E

R

Y

N

Y

Y

I

T

L

P

E

D

A

V

G

E

V

L

L

V

G

G

D

D

I

I

G

A

R

G

S

T

L

L

E

N

G

K

I

L

A

A

A

Q

-

N

L

I

A

D

H

H

R

R

Q

L

E

V

D

L

N

S

G

-

I

-

G

P

N

Q

L

A

K

A

Q

E

I

I

I

L

E

R

Y

D

E

R

F

Q

H

H

Q

Q

Y

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D

-

E

E

D

L

H

N

A

Q

F

F

P

A

V

L

K

H

P

P

Q

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K

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I

I

I

V

H

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D

A

L

M

R

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D

A

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L

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A

N

D

S

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D

D

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I

T

V

L

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C

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D

D

V
x
M

G
|
G

A
x
S

H
x
F

K

H

M

I

W

W

M

I

A
|
A

R
|
R

M

Y

Y

L

N
x
Y

A

S

Q

F

A

R

P

A

N

R

T

Q

C

V

I

M

I

I

S

S

N

N

G

G

F

Q

A
x
Q

S

T

M
|
M

G

G

I

V

A

A

L

L

P

P

G

W

A

A

M

I

A

G

A

A

K

W

L

L

A

V

Q

N

P

P

D

E

Q

R

V

K

A

V

V

V

A

S

V

V

T

S

G
|
G

D
|
D

A
x
G

G

G

F

F

L

L

M

Q

N

S

C

S

Q

M

E

E

I

L

E

E

T

T

A

A

V

V

R

R

L

L

N

K

L

A

A

N

L

V

I

L

V

H

L

L

V

I

W

W

N

V

D
|
D

Q

N

E
x
G

Y
|
Y

G
x
N

L
x
M

I
x
V

K

A

W

I

K
x
Q

Q

E

M

E

N

K

Q

K

F

Y

G

Q

R

R

E

L

S

S

H

G

I

V

Q

E

F

F

A

G

N

P

P

M

D

D

F

F

I

K

K

A

F

Y

A

A

E

E

S

S

F

F

G

G

A

A

K

K

G

G

Y

F

R

A

I

V

N

E

H

S

T

A

D

E

E

A

L

L

L

E

P

P

T

T

L

L

Q

R

Q

A

A

A

I

M

A

D

D

V

N

D

C

G

V

P

V

A

I

V

D

A

C

I

P

P

V

V

D

D

Y
|
Y

S

R

E

D

N

N

L

P

K

L

L

L

T

M

E

G

Q

Q

L

L

active site: I38 (= I22), G40 (= G24), A41 (≠ E25), K42 (≠ E26), I43 (≠ N27), E63 (= E47), T86 (= T70), H125 (= H109), Q126 (= Q110), S127 (≠ V111), Q175 (≠ E159), L268 (≠ T247), E295 (≠ H277), M392 (≠ V377), Q418 (≠ A403), M420 (= M405), D445 (= D430), D472 (= D457), G474 (≠ E459), Y475 (= Y460), M477 (≠ L462), V478 (≠ I463), Q481 (≠ K466), Y541 (= Y526)
binding 3-fluoro-2-oxopropanoic acid: G264 (≠ L243), R265 (≠ S244), Q272 (= Q251), A400 (= A385), R401 (= R386), Y404 (≠ N389)
binding magnesium ion: S135 (≠ Q119), T138 (= T122), D445 (= D430), D472 (= D457), G474 (≠ E459)
binding thiamine diphosphate: G393 (= G378), S394 (≠ A379), F395 (≠ H380), Q418 (≠ A403), M420 (= M405), G444 (= G429), D445 (= D430), G446 (≠ A431), D472 (= D457), G474 (≠ E459), Y475 (= Y460), N476 (≠ G461), M477 (≠ L462), V478 (≠ I463), Y541 (= Y526)

5d6rB Acetolactate synthase from klebsiella pneumoniae in complex with mechanism-based inhibitor
35% identity, 98% coverage: 3:536/543 of query aligns to 7:545/548 of 5d6rB

query
sites
5d6rB

A

G

A

A

E

D

L

L

M

V

V

V

R

S

C

Q

L

L

E

E

N

A

E

Q

E

G

V

V

E

R

Y

Q

I

V

F

F

G

G

I
|
I

P

P

G
|
G

E
x
A

E
x
K

N
x
I

L

D

D

K

L

V

M

F

D

D

A

S

L

L

D

D

S

S

P

S

I

I

K

R

F

I

I

I

L

P

T

V

R

R

H

H

E
|
E

Q

A

G

N

G

A

A

A

F

F

M

M

A

A

D

A

V

A

Y

V

G

G

R
|
R

L

I

T

T

G

G

K

K

A

A

G

G

V

V

C

A

L

L

S

V

T
|
T

L

S

G

G

P

P

G

G

A

C

T

S

N

N

L

L

V

I

T

T

G

G

V

M

A

A

D

T

A

A

N

N

M

S

D

E

R

G

A

D

P

P

V

V

A

A

I

L

A

G

G

G

Q

A

A

V

A

K

T

R

T

A

R

D

L

K

H

A

K

K

E

Q

S

V

H
|
H

Q
|
Q

V
x
S

L

M

D

D

L

T

V

V

N

A

L

M

F

F

Q

S

P

P

I

V

T

T

K

K

Y

Y

S

A

T

I

Q

E

I

V

L

T

S

A

P

P

D

D

I

A

I

L

P

A

E

E

V

V

R

S

K

N

A

A

F

F

K

R

V

A

A

A

Q

E

M

Q

E

G

K

R

P

P

G

G

C

S

S

A

F

F

I

V

D

S

F

L

P

P

E
x
Q

N

D

I

V

A

V

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D

M

G

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D

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K

G

K

K

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L

L

K

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Q

A

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M

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G

A

A

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A

N

P

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D

E

D

K

A

L

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Q

Q

A

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A

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D

K

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I

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A

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Q

A

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K

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N

P

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I

I

F

I

L

A

L

G

G

N

L

G

M

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A

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x
Q

A

P

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A

N

S

S

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K

A

A

L

L

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F

L

A

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A

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F

Y

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A

A

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A

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G

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A

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N

-

Q

-

D

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N

-

F

C

S

R

R

H

F

P

A

L

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S

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I

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G

G

T
x
L

I

F

G

N

L

N

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A

A

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Y

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-

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I

I

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Y

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E

Y

Y

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x
E

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Y

A

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M

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I

I

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R

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active site: I26 (= I22), G28 (= G24), A29 (≠ E25), K30 (≠ E26), I31 (≠ N27), E51 (= E47), T74 (= T70), H113 (= H109), Q114 (= Q110), S115 (≠ V111), Q163 (≠ E159), L254 (≠ T247), E281 (≠ H277), M386 (≠ V377), Q412 (≠ A403), M414 (= M405), D439 (= D430), D466 (= D457), G468 (≠ E459), Y469 (= Y460), M471 (≠ L462), V472 (≠ I463), Q475 (≠ K466), Y535 (= Y526)
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-[(Z)-2-fluoro-1-hydroxy-2-phosphonoethenyl]-5-(2-{[(S)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: M386 (≠ V377), G387 (= G378), S388 (≠ A379), Q412 (≠ A403), M414 (= M405), D439 (= D430), G440 (≠ A431), G468 (≠ E459), Y469 (= Y460), N470 (≠ G461), M471 (≠ L462), Y535 (= Y526)
binding magnesium ion: R63 (= R59), Q212 (≠ R209), D439 (= D430), D466 (= D457), G468 (≠ E459)

1ozgA The crystal structure of klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactor and with an unusual intermediate (see paper)
35% identity, 98% coverage: 3:536/543 of query aligns to 8:548/549 of 1ozgA

query
sites
1ozgA

A

G

A

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active site: I27 (= I22), G29 (= G24), A30 (≠ E25), K31 (≠ E26), I32 (≠ N27), E52 (= E47), T75 (= T70), H114 (= H109), Q115 (= Q110), S116 (≠ V111), Q164 (≠ E159), L257 (≠ T247), E284 (≠ H277), M389 (≠ V377), Q415 (≠ A403), M417 (= M405), D442 (= D430), D469 (= D457), G471 (≠ E459), Y472 (= Y460), M474 (≠ L462), V475 (≠ I463), Q478 (≠ K466), Y538 (= Y526)
binding 2-hydroxyethyl dihydrothiachrome diphosphate: M389 (≠ V377), G390 (= G378), S391 (≠ A379), F392 (≠ H380), Q415 (≠ A403), M417 (= M405), G441 (= G429), D442 (= D430), G443 (≠ A431), D469 (= D457), G471 (≠ E459), Y472 (= Y460), N473 (≠ G461), M474 (≠ L462), V475 (≠ I463), Y538 (= Y526)
binding magnesium ion: D442 (= D430), D469 (= D457), G471 (≠ E459)
binding phosphate ion: G253 (≠ L243), R254 (≠ S244), Q261 (= Q251), R347 (≠ E342), R398 (= R386), Y401 (≠ N389)

1ozfA The crystal structure of klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactors (see paper)
35% identity, 98% coverage: 3:536/543 of query aligns to 7:544/545 of 1ozfA

query
sites
1ozfA

A

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Q

Y

R

D

E

-

L

-

D

-

R

-

G

-

A

E

Q

D

L

H

N

A

Q

F

F

P

A

V

L

K

H

P

P

Q

L

K

R

I

I

I

V

H

R

D

A

L

M

R

Q

S

D

A

I

L

V

A

N

D

S

E

D

D

V

I

T

V

L

V

T

C

V

D

D

V
x
M

G
|
G

A
x
S

H
x
F

K

H

M

I

W

W

M

I

A

A

R
|
R

M

Y

Y
x
L

N
x
Y

A

T

Q

F

A

R

P

A

N

R

T

Q

C

V

I

M

I

I

S

S

N

N

G

G

F

Q

A
x
Q

S

T

M
|
M

G

G

I

V

A

A

L

L

P

P

G

W

A

A

M

I

A

G

A

A

K

W

L

L

A

V

Q

N

P

P

D

E

Q

R

V

K

A

V

V

V

A

S

V

V

T

S

G
|
G

D
|
D

A
x
G

G

G

F

F

L

L

M

Q

N

S

C

S

Q

M

E

E

I

L

E

E

T

T

A

A

V

V

R

R

L

L

N

K

L

A

A

N

L

V

I

L

V

H

L

L

V

I

W

W

N

V

D
|
D

Q

N

E
x
G

Y
|
Y

G
x
N

L
x
M

I
x
V

K

A

W

I

K
x
Q

Q

E

M

E

N

K

Q

K

F

Y

G

Q

R

R

E

L

S

S

H

G

I

V

Q

E

F

F

A

G

N

P

P

M

D

D

F

F

I

K

K

A

F

Y

A

A

E

E

S

S

F

F

G

G

A

A

K

K

G

G

Y

F

R

A

I

V

N

E

H

S

T

A

D

E

E

A

L

L

L

E

P

P

T

T

L

L

Q

R

Q

A

A

A

I

M

A

D

D

V

N

D

C

G

V

P

V

A

I

V

D

A

C

I

P

P

V

V

D

D

Y
|
Y

S

R

E

D

N

N

L

P

K

L

L

L

T

M

E

G

Q

Q

L

L

active site: I26 (= I22), G28 (= G24), A29 (≠ E25), K30 (≠ E26), I31 (≠ N27), E51 (= E47), T74 (= T70), H113 (= H109), Q114 (= Q110), S115 (≠ V111), Q163 (≠ E159), L253 (≠ T247), E280 (≠ H277), M385 (≠ V377), Q411 (≠ A403), M413 (= M405), D438 (= D430), D465 (= D457), G467 (≠ E459), Y468 (= Y460), M470 (≠ L462), V471 (≠ I463), Q474 (≠ K466), Y534 (= Y526)
binding magnesium ion: D438 (= D430), D465 (= D457), G467 (≠ E459)
binding phosphate ion: G249 (≠ L243), R250 (≠ S244), Q257 (= Q251), R343 (≠ E342), R394 (= R386), L396 (≠ Y388), Y397 (≠ N389)
binding thiamine diphosphate: G386 (= G378), S387 (≠ A379), F388 (≠ H380), Q411 (≠ A403), M413 (= M405), G437 (= G429), D438 (= D430), G439 (≠ A431), D465 (= D457), G467 (≠ E459), Y468 (= Y460), N469 (≠ G461), M470 (≠ L462), V471 (≠ I463), Y534 (= Y526)

5dx6A Acetolactate synthase from klebsiella pneumoniae soaked with beta- fluoropyruvate
35% identity, 98% coverage: 3:536/543 of query aligns to 8:540/541 of 5dx6A

query
sites
5dx6A

A

G

A

A

E

D

L

L

M

V

V

V

R

S

C

Q

L

L

E

E

N

A

E

Q

E

G

V

V

E

R

Y

Q

I

V

F

F

G

G

I
|
I

P

P

G
|
G

E
x
A

E
x
K

N
x
I

L

D

D

K

L

V

M

F

D

D

A

S

L

L

D

D

S

S

P

S

I

I

K

R

F

I

I

I

L

P

T

V

R

R

H

H

E
|
E

Q

A

G

N

G

A

A

A

F

F

M

M

A

A

D

A

V

A

Y

V

G

G

R

R

L

I

T

T

G

G

K

K

A

A

G

G

V

V

C

A

L

L

S

V

T
|
T

L

S

G

G

P

P

G

G

A

C

T

S

N

N

L

L

V

I

T

T

G

G

V

M

A

A

D

T

A

A

N

N

M

S

D

E

R

G

A

D

P

P

V

V

A

A

I

L

A

G

G

G

Q

-

A

-

A

A

T

V

T

K

R

R

L

A

H

D

K

K

E

A

S

K

H

-

Q

-

V

-

L

M

D

D

L

T

V

V

N

A

L

M

F

F

Q
x
S

P

P

I

V

T
|
T

K

K

Y

Y

S

A

T

I

Q

E

I

V

L

T

S

A

P

P

D

D

I

A

I

L

P

A

E

E

V

V

R

S

K

N

A

A

F

F

K

R

V

A

A

A

Q

E

M

Q

E

G

K

R

P

P

G

G

C

S

S

A

F

F

I

V

D

S

F

L

P

P

E
x
Q

N

D

I

V

A

V

E

D

M

G

Q

P

I

V

D

S

K

G

K

K

P

V

L

L

K

P

R

-

Q

-

S

A

P

P

S

Q

L

M

P

G

A

A

P

A

N

P

T

D

E

D

K

A

L

I

R

D

Q

Q

A

V

A

A

D

K

I

L

I

I

S

A

Q

Q

A

A

E

K

H

N

P

P

V

I

I

F

I

L

A

L

G

G

N

L

G

M

V

A

I

S

R

Q

A

P

R

E

A

N

S

S

E

K

A

A

L

L

I

R

E

R

F

L

A

L

A

E

K

T

L

S

N

H

I

I

P

P

V

V

A

T

T

S

T

T

F

Y

M

Q

A

A

K

A

G

G

V

A

I

V

P

N

-

Q

-

D

-

N

-

F

C

S

R

R

H

F

P

A

L

G

S

R

I

V

G

G

T
x
L

I

F

G

N

L

N

Q

Q

A

A

H

G

D

D

Y

R

I

L

A

-

C

-

G

-

V

L

D

Q

R

L

A

A

D

D

V

L

V

V

I

I

C

C

V

I

G

G

Y

Y

D

S

I

P

V

V

E

E

Y

Y

H
x
E

P

P

Y

A

L

M

W

W

N

N

K

S

N

G

P

-

N

N

C

A

K

T

I

L

I

V

H

H

I

I

D

D

T

V

Q

L

P

P

A

A

E

Y

V

E

D

E

E

R

Y

N

Y

Y

I

T

L

P

E

D

A

V

G

E

V

L

L

V

G

G

D

D

I

I

G

A

R

G

S

T

L

L

E

N

G

K

I

L

A

A

A

Q

-

N

L

I

A

D

H

H

R

R

Q

L

E

V

D

L

N

S

G

P

I

-

G

-

N

Q

L

A

K

A

Q

E

I

I

I

L

E

R

Y

D

E

R

F

Q

H

H

Q

Q

Y

R

D

E

-

L

-

D

-

R

-

G

-

A

E

Q

D

L

H

N

A

Q

F

F

P

A

V

L

K

H

P

P

Q

L

K

R

I

I

I

V

H

R

D

A

L

M

R

Q

S

D

A

I

L

V

A

N

D

S

E

D

D

V

I

T

V

L

V

T

C

V

D

D

V
x
M

G
|
G

A
x
S

H
x
F

K

H

M

I

W

W

M

I

A

A

R

R

M

Y

Y

L

N

Y

A

S

Q

F

A

R

P

A

N

R

T

Q

C

V

I

M

I

I

S

S

N

N

G

G

F

Q

A
x
Q

S

T

M
|
M