SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_044621645.1 NCBI__GCF_000940995.1:WP_044621645.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

1kofA Crystal structure of gluconate kinase (see paper)
52% identity, 83% coverage: 7:162/189 of query aligns to 10:166/171 of 1kofA

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binding phosphomethylphosphonic acid adenylate ester: S15 (= S12), G16 (≠ A13), G18 (= G15), K19 (= K16), S20 (= S17), A21 (≠ T18), R118 (= R115), L158 (≠ I154)

1ko8A Crystal structure of gluconate kinase (see paper)
52% identity, 83% coverage: 7:162/189 of query aligns to 10:166/172 of 1ko8A

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binding 6-phosphogluconic acid: S15 (= S12), L119 (≠ I116)

1ko5A Crystal structure of gluconate kinase (see paper)
52% identity, 83% coverage: 7:162/189 of query aligns to 10:166/172 of 1ko5A

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binding adenosine-5'-triphosphate: S15 (= S12), G16 (≠ A13), G18 (= G15), K19 (= K16), S20 (= S17), R118 (= R115), R122 (= R119), L158 (≠ I154)

Query Sequence

>WP_044621645.1 NCBI__GCF_000940995.1:WP_044621645.1
MVGKSIIVMGVSASGKSTIGYELAQRIGGKFIDGDDLHPKANILKMAQGEPLNDQDREPW
LERIRDAAFSIESKNETGVIVCSALKKCYREQIREGNHNLAFLYLAGSQEVIRERIRLRQ
GHFMKENMIASQFAVLETPVDETDVITVDIDQSIAAILDSAVAALTEQADTKQAKTEQSM
PATTQEIIA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory