SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_045584537.1 NCBI__GCF_001305595.1:WP_045584537.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3b7wA Crystal structure of human acyl-coa synthetase medium-chain family member 2a, with l64p mutation (see paper)
35% identity, 97% coverage: 18:537/538 of query aligns to 7:534/537 of 3b7wA

query
sites
3b7wA

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active site: T189 (= T193), T332 (= T339), E333 (= E340), N435 (≠ T440), R440 (= R445), K525 (= K528)
binding magnesium ion: M451 (≠ I456), H453 (= H458), V456 (≠ I461)

3c5eA Crystal structure of human acyl-coa synthetase medium-chain family member 2a (l64p mutation) in complex with atp (see paper)
35% identity, 97% coverage: 18:537/538 of query aligns to 6:533/536 of 3c5eA

query
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3c5eA

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active site: T188 (= T193), T331 (= T339), E332 (= E340), N434 (≠ T440), R439 (= R445), K524 (= K528)
binding adenosine-5'-triphosphate: T188 (= T193), S189 (= S194), G190 (= G195), T191 (= T196), S192 (≠ T197), G305 (= G312), E306 (≠ G313), S307 (≠ E314), G329 (= G337), Q330 (= Q338), T331 (= T339), D413 (= D419), F425 (= F431), R428 (= R434), K524 (= K528)
binding magnesium ion: M450 (≠ I456), H452 (= H458), V455 (≠ I461)

3eq6A Crystal structure of human acyl-coa synthetase medium-chain family member 2a (l64p mutation) in a ternary complex with products (see paper)
35% identity, 97% coverage: 18:537/538 of query aligns to 3:530/533 of 3eq6A

query
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3eq6A

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T

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K

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W

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|
W

I

I

G

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L

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|
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|
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|
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|
K

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I

I

Q

R

R

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L

L

R

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D

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active site: T185 (= T193), T328 (= T339), E329 (= E340), N431 (≠ T440), R436 (= R445), K521 (= K528)
binding adenosine monophosphate: G302 (= G312), E303 (≠ G313), S304 (≠ E314), E323 (= E334), S324 (≠ F335), Y325 (= Y336), G326 (= G337), Q327 (= Q338), T328 (= T339), D410 (= D419), F422 (= F431), R425 (= R434), R436 (= R445)
binding Butyryl Coenzyme A: W229 (= W239), F255 (= F265), I277 (≠ T287), V301 (≠ S311), S433 (≠ A442), G434 (= G443), Y435 (= Y444), P501 (≠ A508), Y502 (≠ H509), Y504 (= Y511), R506 (= R513)

2wd9A Crystal structure of human acyl-coa synthetase medium-chain family member 2a (l64p mutation) in complex with ibuprofen (see paper)
35% identity, 97% coverage: 18:537/538 of query aligns to 3:530/533 of 2wd9A

query
sites
2wd9A

R

E

V

V

P

P

D

A

R

K

Y

F

N

N

I

F

G

A

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S

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D

V

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D

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W

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F

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|
I

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L

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|
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V

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L

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T

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T

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G

K
|
K

I

I

I

Q

R

R

R

A

E

K

L

L

R

R

N

D

R

K

active site: T185 (= T193), T328 (= T339), E329 (= E340), N431 (≠ T440), R436 (= R445), K521 (= K528)
binding ibuprofen: I230 (= I240), L231 (≠ G241), G326 (= G337), Q327 (= Q338), T328 (= T339), R436 (= R445)

2vzeA Crystal structure of human acyl-coa synthetase medium-chain family member 2a (l64p mutation) in complex with amp (see paper)
35% identity, 97% coverage: 18:537/538 of query aligns to 3:530/533 of 2vzeA

query
sites
2vzeA

R

E

V

V

P

P

D

A

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K

Y

F

N

N

I

F

G

A

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|
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D

G

D

D

K

G

G

N

N

R

V

L

L

P

P

P

P

G

G

Q

T

I

E

G

G

L

D

I

I

A

G

V

I

H

-

R

R

P

V

D

K

P

P

V

I

-

R

-

P

-

I

-

G

M

I

F

F

L

S

G

G

Y

Y

W

V

N

D

N

N

P

P

D

D

A

K

T

T

A

A

A

A

K

N

F

I

I

R

G

G

N

D

W

F

L

W

V

L

T

L

G

G

D
|
D

Q

R

G

G

E

I

L

K

D

D

Q

E

D

D

G

G

Y

Y

I

F

R

Q

F
|
F

V

M

G

G

R
|
R

D

A

D

D

D

D

V

I

I

I

T
x
N

S

S

A

S

G

G

Y

Y

R
|
R

I

I

G

G

P

P

G

S

E

E

I

V

E

E

D

N

C

A

L

L

I

M

G

E

H

H

P

P

A

A

I

V

R

V

M

E

A

T

A

A

V

V

V

I

G

S

V

S

P

P

D

D

P

P

L

V

R

R

T

G

E

E

I

V

V

V

K

K

A

A

F

F

V

V

V

V

L

L

Q

A

E

S

D

Q

V

F

V

L

P

S

D

H

D

D

-

P

-

E

A

Q

L

L

V

T

A

K

S

E

I

L

Q

Q

Q

Q

H

H

V

V

K

K

T

S

K

V

L

T

A

A

A

P

H

Y

E

K

Y

Y

P

P

R

R

A

K

I

I

E

E

F

F

V

V

E

L

S

N

L

L

P

P

M

K

T

T

T

V

T

T

G

G

K
|
K

I

I

I

Q

R

R

R

A

E

K

L

L

R

R

N

D

R

K

active site: T185 (= T193), T328 (= T339), E329 (= E340), N431 (≠ T440), R436 (= R445), K521 (= K528)
binding adenosine monophosphate: W229 (= W239), G302 (= G312), E303 (≠ G313), S304 (≠ E314), E323 (= E334), Y325 (= Y336), G326 (= G337), Q327 (= Q338), T328 (= T339), D410 (= D419), F422 (= F431), R425 (= R434), R436 (= R445)

Q08AH3 Acyl-coenzyme A synthetase ACSM2A, mitochondrial; Acyl-CoA synthetase medium-chain family member 2A; Benzoate--CoA ligase; Butyrate--CoA ligase 2A; Butyryl-coenzyme A synthetase 2A; Middle-chain acyl-CoA synthetase 2A; EC 6.2.1.2; EC 6.2.1.25 from Homo sapiens (Human) (see 4 papers)
35% identity, 97% coverage: 18:537/538 of query aligns to 39:566/577 of Q08AH3

query
sites
Q08AH3

R

E

V

V

P

P

D

A

R

K

Y

F

N

N

I

F

G

A

V

S

D

D

V

V

C

L

D

D

R

H

W

W

A

A

E

D

A

M

E

E

P

K

-

A

-

G

D

K

R

R

L

L

-

P

-

S

-

P

A

A

L

L

I

W

H

W

M

V

R

N

R

G

D

K

G

G

G

K

A

E

E

L

E

M

Y

W

R

N

F

F

A

R

D

E

I

L

R

S

A

E

L

N

S

S

N

Q

R

Q

F

A

A

A

N

N

A

V

L

L

D

S

-

G

A

A

Q

C

G

G

V

L

V

Q

R

R

G

G

D

D

R

R

V

V

G

A

I

V

L

V

L

L

P

P

Q

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A

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P

P

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W

A

W

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L

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V

H

I

V

L

A

G

V

C

Y

I

K

R

L

A

G

G

G

L

V

I

A

F

V

M

P

P

L

G

F

T

S

I

L
x
Q

F

M

G

K

V

S

E

T

A

D

L

I

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L

Y

Y

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R

L

L

A

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N

M

C

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K

A

A

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K

A

A

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I

V

V

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A

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G

A

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A

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G

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A

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A

Q

K

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A

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A

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L

C

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P

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L

L

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R

A

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K

F

L

R

L

I

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-

S

D

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G

K

S

S

G

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P

D

G

G

C

W

L

L

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N

W

F

H

K

G

K

L

L

C

L

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N

A

E

A

A

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S

D

T

D

T

F

H

T

H

P

C

A

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T

G

G

P

S

D

Q

D

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P

A

A

S

L

A

I

I

I

Y

Y

F

T
|
T

S
|
S

G
|
G

T
|
T

T
x
S

G
|
G

Q
x
L

P
|
P

K
|
K

G

M

A

A

L

E

H

H

A

S

H

Y

R

S

V

S

L

L

L

-

G

-

H

-

L

-

P

-

G

G

V

L

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K

M

A

S

K

H

M

D

D

-

A

-

G

-

W

L

T

F

G

P

L

Q

Q

P

A

G

S

D

D

R

I

I

M

W

W

T

T

P

I

A

S

D

D

W

T

A

G

W

W

I

I

G

L

G

N

L

I

L

L

D

C

V

S

L

L

L

M

P

E

A

P

W

W

H

A

H

L

G

G

V

A

T

C

V

T

V

F

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V

H

H

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L

F

L

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K

K

F

F

D

D

A

P

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L

A

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A

I

F

L

A

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L

T

L

L

A

S

E

S

F

Y

Q

P

V

I

R

K

N

S

A

M

F

M

L

G

P

A

P

P

T

I

A

V

L

Y

K

R

M

M

M

L

R

L

A

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V

Q

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D

D

L

P

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G

S

A

Y

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F

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I

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N

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C

V

V

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G

G

G

E

E

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L

L

L

G

P

A

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G

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L

L

L

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N

W

W

G

R

R

A

Q

Q

T

T

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-

G

G

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L

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D

I

I

N

R

E
|
E

F
x
S

Y
|
Y

G
|
G

Q
|
Q

T
|
T

E

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C

T

N

G

M

L

I

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V

C

S

M

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K

T

T

L

-

M

M

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I

K

K

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G

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M

M

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A

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D

D

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V

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V

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I

I

D

D

G

D

D

K

G

G

N

N

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L

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P

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K

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G

M

I

F

F

L

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G

G

Y

Y

W

V

N

D

N

N

P

P

D

D

A

K

T

T

A

A

A

A

K

N

F

I

I

R

G

G

N

D

W

F

L

W

V

L

T

L

G

G

D
|
D

Q

R

G

G

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I

L

K

D

D

Q

E

D

D

G

G

Y

Y

I

F

R

Q

F

F

V

M

G

G

R
|
R

D

A

D

N

D

D

V

I

I

I

T

N

S

S

A
x
S

G
|
G

Y
|
Y

R
|
R

I

I

G

G

P

P

G

S

E

E

I

V

E

E

D

N

C

A

L

L

I

M

G

E

H

H

P

P

A

A

I

V

R

V

M

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A

T

A

A

V

V

V

I

G

S

V

S

P

P

D

D

P

P

L

V

R
|
R

T

G

E

E

I

V

V

V

K

K

A

A

F

F

V

V

V

V

L

L

Q

A

E
x
S

D

Q

V

F

V

L

P

S

D

H

D

D

-

P

-

E

A

Q

L

L

V

T

A

K

S

E

I

L

Q

Q

Q

Q

H

H

V

V

K
|
K

T

S

K

V

L

T

A

A

A

P

H

Y

E

K

Y
|
Y

P
|
P

R
|
R

A

K

I

I

E

E

F

F

V

V

E

L

S

N

L

L

P

P

M

K

T

T

T

V

T

T

G

G

K
|
K

I

I

I

Q

R

R

R

A

E

K

L

L

R

R

N

D

R

K

Q139 (≠ L112) binding CoA
221:229 (vs. 193:201, 78% identical) binding ATP
ESYGQT 359:364 (≠ EFYGQT 334:339) binding ATP
T364 (= T339) binding substrate
D446 (= D419) binding ATP
R461 (= R434) binding ATP
SGY 469:471 (≠ AGY 442:444) binding CoA
R472 (= R445) binding substrate
R501 (= R474) binding CoA
S513 (≠ E486) to L: in dbSNP:rs1133607
K532 (= K503) binding CoA
YPR 540:542 (= YPR 511:513) binding CoA
K557 (= K528) binding ATP

3gpcA Crystal structure of human acyl-coa synthetase medium-chain family member 2a (l64p mutation) in a complex with coa (see paper)
35% identity, 97% coverage: 18:537/538 of query aligns to 4:529/532 of 3gpcA

query
sites
3gpcA

R

E

V

V

P

P

D

A

R

K

Y

F

N

N

I

F

G

A

V

S

D

D

V

V

C

L

D

D

R

H

W

W

A

A

E

D

A

M

E

E

-

K

-

A

-

G

-

K

-

R

P

P

D

P

R

S

L

P

A

A

L

L

I

W

H

W

M

V

R

N

R

G

D

K

G

G

G

K

A

E

E

L

E

M

Y

W

R

N

F

F

A

R

D

E

I

L

R

S

A

E

L

N

S

S

N

Q

R

Q

F

A

A

A

N

N

A

V

L

L

D

S

-

G

A

A

Q

C

G

G

V

L

V

Q

R

R

G

G

D

D

R

R

V

V

G

A

I

V

L

V

L

L

P

P

Q

R

A

V

P

P

E

E

T

W

A

W

V

L

S

V

H

I

V

L

A

G

V

C

Y

I

K

R

L

A

G

G

G

L

V

I

A

F

V

M

P

P

L

G

F

T

S

I

L

Q

F

M

G

K

V

S

E

T

A

D

L

I

E

L

Y

Y

R

R

L

L

A

Q

N

M

C

S

G

K

A

A

R

K

A

A

V

I

V

V

T

A

D

G

A

D

A

E

G

V

A

I

A

Q

K

E

I

V

A

D

Q

T

I

V

R

A

D

S

R

E

L

C

P

P

E

S

L

L

R

R

A

I

V

K

F

L

R

L

I

V

-

S

D

E

G

K

S

S

G

C

P

D

G

G

C

W

L

L

D

N

W

F

H

K

G

K

L

L

C

L

D

N

A

E

A

A

S

S

D

T

D

T

F

H

T

H

P

C

A

V

D

E

T

T

G

G

P

S

D

Q

D

E

P

A

A

S

L

A

I

I

I

Y

Y

F

T
|
T

S

S

G

G

T

-

T

-

G

G

Q

L

P

P

K

K

G

M

A

A

L

E

H

H

A

S

H

Y

R

S

V

S

L

L

L

-

G

-

H

-

L

-

P

-

G

G

V

L

E

K

M

A

S

K

H

M

D

D

-

A

-

G

-

W

L

T

F

G

P

L

Q

Q

P

A

G

S

D

D

R

I

I

M

W

W

T

T

P

I

A

S

D

D

W

T

A

G

W

W

I

I

G

L

G

N

L

I

L

L

D

C

V

S

L

L

L

M

P

E

A

P

W

W

H

A

H

L

G

G

V

A

T

C

V

T

V

F

S

V

H

H

R

L

F

L

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K

K

F

F

D

D

A

P

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L

A

V

A

I

F

L

A

K

L

T

L

L

A

S

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F

Y

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I

R

K

N

S

A

M

F

M

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G

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A

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L

Y

K

R

M

M

M

L

R

L

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Q

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D

D

L

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G

S

A

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H

F

A

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I

H

A

L

M

Q

R

N

S

C

V

V

A

T

S

V

G
|
G

G
x
E

E
x
S

T

L

L

L

G

P

A

E

G

T

L

L

L

E

D

N

W

W

G

R

R

A

Q

Q

T

T

F

-

G

G

V

L

T

D

I

I

N

R

E
|
E

F

S

Y
|
Y

G
|
G

Q
|
Q

T
|
T

E
|
E

C

T

N

G

M

L

I

T

V

C

S

M

S

V

A

S

A

K

T

T

L

-

M

M

Q

K

P

I

K

K

P

P

G

G

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Y

M

M

G

G

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A

P

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G

C

H

Y

D

D

V

V

A

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V

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I

I

D

D

G

D

D

K

G

G

N

N

R

V

L

L

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P

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G

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I

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R

R

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D

K

P

P

V

I

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R

-

P

-

I

-

G

M

I

F

F

L

S

G

G

Y

Y

W

V

N

D

N

N

P

P

D

D

A

K

T

T

A

A

A

A

K

N

F

I

I

R

G

G

N

D

W

F

L

W

V

L

T

L

G

G

D
|
D

Q

R

G

G

E

I

L

K

D

D

Q

E

D

D

G

G

Y

Y

I

F

R

Q

F
|
F

V

M

G

G

R
|
R

D

A

D

D

D

D

V

I

I

I

T
x
N

S

S

A

S

G

G

Y

Y

R
|
R

I

I

G

G

P

P

G

S

E

E

I

V

E

E

D

N

C

A

L

L

I

M

G

E

H
|
H

P

P

A

A

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x
V

R

V

M

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A

T

A

A

V

V

V

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G

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V

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P

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D

P

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L

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K

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V

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L

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A

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D

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P

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L

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L

L

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K

T
|
T

T

V

T

T

G

G

K
|
K

I

I

I
x
Q

R

R

R

A

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K

L

L

R

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R

K

active site: T186 (= T193), T327 (= T339), E328 (= E340), N430 (≠ T440), R435 (= R445), K520 (= K528)
binding coenzyme a: G301 (= G312), E302 (≠ G313), S303 (≠ E314), E322 (= E334), Y324 (= Y336), G325 (= G337), Q326 (= Q338), T327 (= T339), D409 (= D419), F421 (= F431), R424 (= R434), T516 (= T524), K520 (= K528), Q522 (≠ I530)
binding magnesium ion: H448 (= H458), V451 (≠ I461)

3dayA Crystal structure of human acyl-coa synthetase medium-chain family member 2a (l64p mutation) in complex with amp-cpp (see paper)
35% identity, 97% coverage: 18:537/538 of query aligns to 7:532/535 of 3dayA

query
sites
3dayA

R

E

V

V

P

P

D

A

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Y

F

N

N

I

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A

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D

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W

A

A

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-

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-

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-

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-

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A

A

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R

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A

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-

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-

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-

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D

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Y

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active site: T189 (= T193), T332 (= T339), E333 (= E340), N435 (≠ T440), R440 (= R445), K523 (= K528)
binding diphosphomethylphosphonic acid adenosyl ester: T189 (= T193), S190 (= S194), G191 (= G195), T192 (= T196), S193 (≠ T197), K197 (= K201), G306 (= G312), E307 (≠ G313), S308 (≠ E314), Y329 (= Y336), G330 (= G337), Q331 (= Q338), T332 (= T339), D414 (= D419), F426 (= F431), R429 (= R434), K523 (= K528)
binding magnesium ion: M451 (≠ I456), H453 (= H458), V456 (≠ I461)

8bitA Crystal structure of acyl-coa synthetase from metallosphaera sedula in complex with coenzyme a and acetyl-amp
33% identity, 99% coverage: 3:534/538 of query aligns to 17:543/562 of 8bitA

query
sites
8bitA

P

P

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Y

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