SitesBLAST
Comparing WP_045585170.1 NCBI__GCF_001305595.1:WP_045585170.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
O85673 Anthranilate 1,2-dioxygenase large subunit; EC 1.14.12.1 from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) (see 2 papers)
32% identity, 86% coverage: 27:395/429 of query aligns to 27:403/471 of O85673
- M43 (= M43) mutation to K: Prevents anthranilate degradation.
- D217 (= D215) mutation to A: In ACN476; loss of dioxygenase activity and 2-fold lower redox potential.; mutation to E: Loss of dioxygenase activity and lack of iron at the mononuclear site.; mutation to N: Loss of dioxygenase activity.
2xrxA Crystal structure of biphenyl dioxygenase in complex with biphenyl from burkholderia xenovorans lb400 (see paper)
29% identity, 93% coverage: 32:428/429 of query aligns to 22:423/432 of 2xrxA
- active site: H106 (= H116), D203 (= D215), H206 (= H218), H212 (≠ F226), D361 (= D371)
- binding biphenyl: Q199 (≠ N212), F200 (vs. gap), D203 (= D215), H206 (= H218), H296 (≠ N308), L306 (≠ S318), F309 (= F321), F357 (≠ G367)
- binding fe (ii) ion: Q199 (≠ N212), H206 (= H218), H212 (≠ F226), D361 (= D371)
- binding fe2/s2 (inorganic) cluster: C83 (= C93), H85 (= H95), R86 (= R96), C103 (= C113), Y105 (= Y115), H106 (= H116), W108 (= W118)
2xshA Crystal structure of p4 variant of biphenyl dioxygenase from burkholderia xenovorans lb400 in complex with 2,6 di chlorobiphenyl (see paper)
29% identity, 93% coverage: 32:428/429 of query aligns to 22:424/433 of 2xshA
- active site: H106 (= H116), D204 (= D215), H207 (= H218), H213 (≠ F226), D362 (= D371)
- binding 2,6-dichlorobiphenyl: F201 (vs. gap), M205 (≠ G216), H207 (= H218), Q296 (≠ F307), H297 (≠ N308), L307 (vs. gap), F358 (≠ G367)
- binding fe (ii) ion: Q200 (≠ N212), H207 (= H218), H213 (≠ F226), D362 (= D371)
- binding fe2/s2 (inorganic) cluster: C83 (= C93), H85 (= H95), R86 (= R96), C103 (= C113), Y105 (= Y115), H106 (= H116), W108 (= W118)
2yflA Crystal structure of biphenyl dioxygenase variant rr41 with 2-chloro dibenzofuran (see paper)
29% identity, 93% coverage: 32:428/429 of query aligns to 22:424/433 of 2yflA
- active site: H106 (= H116), D204 (= D215), H207 (= H218), H213 (≠ F226), D362 (= D371)
- binding 2-chlorodibenzofuran: Q200 (≠ N212), D204 (= D215), M205 (≠ G216), H207 (= H218), S257 (≠ D265), H297 (≠ N308), L307 (vs. gap), F352 (≠ Q361)
- binding fe (ii) ion: Q200 (≠ N212), H207 (= H218), H213 (≠ F226), D362 (= D371)
- binding fe2/s2 (inorganic) cluster: C83 (= C93), H85 (= H95), R86 (= R96), C103 (= C113), Y105 (= Y115), H106 (= H116), W108 (= W118)
2yfjA Crystal structure of biphenyl dioxygenase variant rr41 with dibenzofuran (see paper)
29% identity, 93% coverage: 32:428/429 of query aligns to 22:424/433 of 2yfjA
- active site: H106 (= H116), D204 (= D215), H207 (= H218), H213 (≠ F226), D362 (= D371)
- binding dibenzofuran: Q200 (≠ N212), F201 (vs. gap), D204 (= D215), M205 (≠ G216), H207 (= H218), A208 (≠ F219), H297 (≠ N308), L307 (vs. gap), F358 (≠ G367)
- binding fe (ii) ion: Q200 (≠ N212), H207 (= H218), H213 (≠ F226), D362 (= D371)
- binding fe2/s2 (inorganic) cluster: C83 (= C93), H85 (= H95), R86 (= R96), C103 (= C113), Y105 (= Y115), H106 (= H116), W108 (= W118)
7q05E Crystal structure of tpado in complex with tpa (see paper)
31% identity, 93% coverage: 27:427/429 of query aligns to 13:390/409 of 7q05E
- binding fe2/s2 (inorganic) cluster: C80 (= C93), H82 (= H95), R83 (= R96), C100 (= C113), Y102 (= Y115), H103 (= H116), W105 (= W118)
- binding terephthalic acid: V203 (≠ T213), A209 (≠ F219), S241 (≠ M257), L271 (≠ R296), I273 (= I298), R292 (= R323), D339 (= D371)
7q06D Crystal structure of tpado in complex with 2-oh-tpa (see paper)
30% identity, 93% coverage: 27:427/429 of query aligns to 13:385/389 of 7q06D
- binding 2-Hydroxyterephthalic acid: V203 (≠ T213), S206 (≠ G216), A209 (≠ F219), F216 (= F226), N222 (≠ E235), S241 (≠ V256), I268 (= I298), R287 (= R323)
- binding fe (iii) ion: H208 (= H218), H213 (= H223), D334 (= D371)
- binding fe2/s2 (inorganic) cluster: C80 (= C93), H82 (= H95), R83 (= R96), C100 (= C113), Y102 (= Y115), H103 (= H116), W105 (= W118)
5aeuA Crystal structure of ii9 variant of biphenyl dioxygenase from burkholderia xenovorans lb400 (see paper)
29% identity, 93% coverage: 32:428/429 of query aligns to 22:424/433 of 5aeuA
- active site: H106 (= H116), D204 (= D215), H207 (= H218), H213 (≠ F226), D362 (= D371)
- binding fe (ii) ion: H207 (= H218), H213 (≠ F226), D362 (= D371)
- binding fe2/s2 (inorganic) cluster: C83 (= C93), H85 (= H95), R86 (= R96), M88 (≠ A98), C103 (= C113), Y105 (= Y115), H106 (= H116), W108 (= W118)
7vjuA Crystal structure of terephthalate dioxygenase from comamonas testosteroni kf1 (see paper)
29% identity, 93% coverage: 27:427/429 of query aligns to 12:374/378 of 7vjuA
7q04D Crystal structure of tpado in a substrate-free state (see paper)
30% identity, 93% coverage: 27:427/429 of query aligns to 12:371/374 of 7q04D
Q53122 Biphenyl 2,3-dioxygenase subunit alpha; Biphenyl dioxygenase system, oxygenase component subunit alpha; BDO, oxygenase component subunit alpha; Rieske dioxygenase; Terminal oxygenase component of biphenyl dioxygenase, large subunit; EC 1.14.12.18 from Rhodococcus jostii (strain RHA1) (see paper)
37% identity, 50% coverage: 4:218/429 of query aligns to 3:224/460 of Q53122
- C98 (= C93) binding [2Fe-2S] cluster
- H100 (= H95) binding [2Fe-2S] cluster
- C118 (= C113) binding [2Fe-2S] cluster
- H121 (= H116) binding [2Fe-2S] cluster
- H224 (= H218) binding Fe cation
Sites not aligning to the query:
- 217:230 binding substrate
- 230 binding Fe cation
- 378 binding Fe cation
1uliC Biphenyl dioxygenase (bpha1a2) derived from rhodococcus sp. Strain rha1 (see paper)
29% identity, 96% coverage: 18:429/429 of query aligns to 5:413/425 of 1uliC