SitesBLAST

3op4A Crystal structure of putative 3-ketoacyl-(acyl-carrier-protein) reductase from vibrio cholerae o1 biovar eltor str. N16961 in complex with NADP+ (see paper)
40% identity, 100% coverage: 1:248/249 of query aligns to 4:246/247 of 3op4A

query
sites
3op4A

M

M

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E

N

G

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K

V

V

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A

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T

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G

A

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x
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M

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binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G12), S17 (≠ A14), R18 (= R17), I20 (= I19), T40 (≠ A44), N62 (≠ D61), V63 (= V62), N89 (= N88), A90 (= A89), I92 (= I91), V139 (≠ I138), S141 (= S140), Y154 (= Y155), K158 (= K159), P184 (= P185), G185 (= G186), I187 (= I188), T189 (= T190), M191 (≠ I192)

4i08A Crystal structure of beta-ketoacyl-acyl carrier protein reductase (fabg) from vibrio cholerae in complex with NADPH (see paper)
40% identity, 100% coverage: 1:248/249 of query aligns to 4:242/243 of 4i08A

query
sites
4i08A

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active site: G19 (= G18), N113 (≠ S112), S141 (= S140), Q151 (≠ G152), Y154 (= Y155), K158 (= K159)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G15 (= G12), S17 (≠ A14), R18 (= R17), I20 (= I19), T40 (≠ A44), N62 (≠ D61), V63 (= V62), N89 (= N88), A90 (= A89), G140 (≠ S139), S141 (= S140), Y154 (= Y155), K158 (= K159), P184 (= P185), G185 (= G186), T189 (= T190)

7emgB Carbonyl reductase variant 4 (r123c/l209p/f183y/v61k) from serratia marcescens complexed with NADP+ (see paper)
40% identity, 98% coverage: 6:248/249 of query aligns to 5:242/243 of 7emgB

query
sites
7emgB

K

K

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G

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binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (= G12), S13 (≠ A14), R14 (= R17), T36 (≠ A44), N58 (≠ D61), V59 (= V62), I88 (= I91)

4nbuB Crystal structure of fabg from bacillus sp (see paper)
38% identity, 99% coverage: 3:248/249 of query aligns to 5:244/244 of 4nbuB

query
sites
4nbuB

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active site: G18 (= G18), N111 (≠ S112), S139 (= S140), Q149 (≠ G152), Y152 (= Y155), K156 (= K159)
binding acetoacetyl-coenzyme a: D93 (≠ P94), K98 (≠ D99), S139 (= S140), N146 (≠ G147), V147 (≠ F150), Q149 (≠ G152), Y152 (= Y155), F184 (≠ L187), M189 (≠ I192), K200 (≠ E204)
binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G12), N17 (≠ S15), G18 (= G18), I19 (= I19), D38 (= D38), F39 (≠ L39), V59 (≠ C60), D60 (= D61), V61 (= V62), N87 (= N88), A88 (= A89), G89 (= G90), I90 (= I91), T137 (≠ I138), S139 (= S140), Y152 (= Y155), K156 (= K159), P182 (= P185), F184 (≠ L187), T185 (≠ I188), T187 (= T190), M189 (≠ I192)

4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
41% identity, 100% coverage: 1:248/249 of query aligns to 1:247/247 of 4jroC

query
sites
4jroC

M

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A

G

Q

L

R

I

G

G

G

N

G

A

I

-

F

-

G

G

G
x
Q

P

A

H

N

Y
|
Y

S

V

A

A

A

S

K
|
K

A

A

G

G

V

V

L

I

G

G

L

L

A

T

K

K

A

T

M

T

A

A

R

R

E

E

L

L

G

A

P

P

D

R

N

G

V

I

R

N

V

V

N

N

S

A

V

V

T

A

P
|
P

G

G

L

F

I
|
I

Q

T

T
|
T

D

D

I

M

T

T

G

-

G

D

K

K

L

L

T

D

D

E

E

K

L

T

R

K

T

E

E

A

I

M

L

L

K

A

G

Q

I

I

P

P

L

L

N

G

R

A

L

Y

G

G

D

T

A

T

E

E

D

D

V

I

A

A

R

N

S

A

C

V

L

L

F

F

L

L

A

A

S

S

E

D

L

A

S

S

S

K

Y

Y

I

I

T

T

G

G

A

Q

T

T

L

L

D

S

V

V

N

D

G

G

M

M

L

V

I

M

active site: G16 (= G18), S142 (= S140), Q152 (≠ G152), Y155 (= Y155), K159 (= K159)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G12), S14 (≠ A14), R15 (= R17), G16 (= G18), I17 (= I19), N35 (≠ D38), Y36 (≠ L39), N37 (≠ D40), G38 (= G41), S39 (≠ A42), N63 (≠ F58), V64 (≠ A59), N90 (= N88), A91 (= A89), I93 (= I91), I113 (≠ V111), S142 (= S140), Y155 (= Y155), K159 (= K159), P185 (= P185), I188 (= I188), T190 (= T190)

P0AEK2 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Escherichia coli (strain K12) (see 2 papers)
38% identity, 100% coverage: 1:248/249 of query aligns to 1:243/244 of P0AEK2

query
sites
P0AEK2

M

M

L

N

L

F

S

E

N

G

K

K

V

I

C

A

V

L

I

V

T

T

G
|
G

A
|
A

A
x
S

S

-

R

-

R
|
R

G

G

I

I

G

G

K

R

A

A

T

I

A

A

R

E

L

T

F

L

A

A

L

A

H

R

G

G

G

A

R

K

A

V

I

I

-

G

-

T

I

A

L
x
T

D

S

L

E

D

N

G

G

A

A

Q

Q

A

A

A

I

E

-

A

-

A

S

A

D

E

Y

L

L

G

G

E

A

A

N

H

G

R

K

G

G

F

L

A

M

C

L

D
x
N

V
|
V

T

T

D

D

R

P

D

A

A

S

C

I

F

E

A

S

A

V

A

L

T

E

R

K

V

I

V

R

E

A

E

E

F

F

G

G

R

E

I

V

D

D

V

I

L

L

V

V

N

N

N
|
N

A

A

G

G

I

I

T

T

Q

R

P

D

L

N

K

L

F

L

M

M

D

R

I

M

G

K

P

D

K

E

N

E

Y

W

E

N

A

D

V

I

T

I

D

E

V

T

S

N

L

L

R

S

G

S

T

V

L

F

Y

R

M

L

S

S

Q

K

A

A

V

V

V

M

P

R

H

A

M

M

R

M

E

K

R

K

K

R

A

H

G

G

S

R

I

I

V

I

C

T

I

I

S

G

S

S

V

V

S

V

A

G

Q

T

R

M

G

G

G

N

G

G

I

-

F

-

G

G

G

Q

P

A

H

N

Y
|
Y

S
x
A

A
|
A

K
|
K

A

A

G

G

V

L

L

I

G

G

L

F

A

S

K

K

A

S

M

L

A

A

R

R

E

E

L

V

G

A

P

S

D

R

N

G

V

I

R

T

V

V

N

N

S

V

V

V

T

A

P

P

G

G

L

F

I
|
I

Q

E

T

T

D

D

I

M

T

T

G

R

G

A

K

-

L

L

T

S

D

D

E

D

L

Q

R

R

T

A

E

G

I

I

L

L

K

A

G

Q

I

V

P

P

L

A

N

G

R

R

L

L

G

G

D

G

A

A

E

Q

D

E

V

I

A

A

R

N

S

A

C

V

L

A

F

F

L

L

A

A

S

S

E

D

L

E

S

A

Y

Y

I

I

T

T

G

G

A
x
E

T

T

L

L

D

H

V

V

N

N

G

G

M

M

L

Y

I

M

GAS--R 12:15 (≠ GAASRR 12:17) binding NADP(+)
T37 (≠ L37) binding NADP(+)
NV 59:60 (≠ DV 61:62) binding NADP(+)
N86 (= N88) binding NADP(+)
Y151 (= Y155) mutation to F: Defect in the affinity for NADPH.
YAAAK 151:155 (≠ YSAAK 155:159) binding NADP(+)
A154 (= A158) mutation to T: Decreases in the thermolability of the reductase; when associated with K-233.
K155 (= K159) mutation to A: Defect in the affinity for NADPH.
I184 (= I188) binding NADP(+)
E233 (≠ A238) mutation to K: Decreases in the thermolability of the reductase; when associated with T-154.

1q7bA The structure of betaketoacyl-[acp] reductase from e. Coli in complex with NADP+ (see paper)
39% identity, 98% coverage: 6:248/249 of query aligns to 5:242/243 of 1q7bA

query
sites
1q7bA

K

K

V

I

C

A

V

L

I

V

T

T

G
|
G

A

A

A
x
S

S

-

R

-

R
|
R

G
|
G

I

I

G

G

K

R

A

A

T

I

A

A

R

E

L

T

F

L

A

A

L

A

H

R

G

G

G

A

R

K

A

V

I

I

-

G

-

T

I

A

L
x
T

D

S

L

E

D

N

G

G

A

A

Q

Q

A

A

A

I

E

-

A

-

A

S

A

D

E

Y

L

L

G

G

E

A

A

N

H

G

R

K

G

G

F

L

A

M

C

L

D
x
N

V
|
V

T

T

D

D

R

P

D

A

A

S

C

I

F

E

A

S

A

V

A

L

T

E

R

K

V

I

V

R

E

A

E

E

F

F

G

G

R

E

I

V

D

D

V

I

L

L

V

V

N

N

N
|
N

A
|
A

G
|
G

I
|
I

T

T

Q

R

P

D

L

N

K

L

F

L

M

M

D

R

I

M

G

K

P

D

K

E

N
x
E

Y

W

E

N

A

D

V

I

T

I

D

E

V

T

S

N

L

L

R

S

G

S

T

V

L

F

Y

R

M

L

S

S

Q

K

A

A

V

V

V

M

P

R

H

A

M

M

R

M

E

K

R

K

K

R

A

H

G

G

S

R

I

I

V

I

C

T

I

I

S

G

S
|
S

V

V

S

V

A

G

Q

T

R

M

G

G

G

N

G

G

I

-

F

-

G

G

G
x
Q

P

A

H

N

Y
|
Y

S

A

A

A

K
|
K

A

A

G

G

V

L

L

I

G

G

L

F

A

S

K

K

A

S

M

L

A

A

R

R

E

E

L

V

G

A

P

S

D

R

N

G

V

I

R

T

V

V

N

N

S

V

V

V

T

A

P
|
P

G
|
G

L

F

I
|
I

Q

E

T

T

D

D

I

M

T

T

G

R

G

A

K

-

L

L

T

S

D

D

E

D

L

Q

R

R

T

A

E

G

I

I

L

L

K

A

G

Q

I

V

P

P

L

A

N

G

R

R

L

L

G

G

D

G

A

A

E

Q

D

E

V

I

A

A

R

N

S

A

C

V

L

A

F

F

L

L

A

A

S

S

E

D

L

E

S

A

Y

Y

I

I

T

T

G

G

A
x
E

T
|
T

L

L

D

H

V

V

N

N

G

G

M

M

L

Y

I

M

active site: G15 (= G18), E101 (≠ N104), S137 (= S140), Q147 (≠ G152), Y150 (= Y155), K154 (= K159)
binding calcium ion: E232 (≠ A238), T233 (= T239)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G12), S13 (≠ A14), R14 (= R17), T36 (≠ L37), N58 (≠ D61), V59 (= V62), N85 (= N88), A86 (= A89), G87 (= G90), I88 (= I91), S137 (= S140), Y150 (= Y155), K154 (= K159), P180 (= P185), G181 (= G186), I183 (= I188)

1q7cA The structure of betaketoacyl-[acp] reductase y151f mutant in complex with NADPH fragment (see paper)
38% identity, 98% coverage: 6:248/249 of query aligns to 5:242/243 of 1q7cA

query
sites
1q7cA

K

K

V

I

C

A

V

L

I

V

T

T

G
|
G

A

A

A
x
S

S

-

R

-

R
|
R

G
|
G

I

I

G

G

K

R

A

A

T

I

A

A

R

E

L

T

F

L

A

A

L

A

H

R

G

G

G

A

R

K

A

V

I

I

-

G

-

T

I
x
A

L
x
T

D

S

L

E

D

N

G

G

A

A

Q

Q

A

A

A

I

E

-

A

-

A

S

A

D

E

Y

L

L

G

G

E

A

A

N

H

G

R

K

G

G

F

L

A

M

C
x
L

D
x
N

V
|
V

T

T

D

D

R

P

D

A

A

S

C

I

F

E

A

S

A

V

A

L

T

E

R

K

V

I

V

R

E

A

E

E

F

F

G

G

R

E

I

V

D

D

V

I

L

L

V

V

N

N

A

A

G
|
G

I
|
I

T

T

Q

R

P

D

L

N

K

L

F

L

M

M

D

R

I

M

G

K

P

D

K

E

N

E

Y

W

E

N

A

D

V

I

T

I

D

E

V

T

S

N

L

L

R

S

G

S

T

V

L

F

Y

R

M

L

S

S

Q

K

A

A

V

V

V

M

P

R

H

A

M

M

R

M

E

K

R

K

K

R

A

H

G

G

S

R

I

I

V

I

C

T

I

I

S

G

S
|
S

V

V

S

V

A

G

Q

T

R

M

G

G

G

N

G

G

I

-

F

-

G

G

G
x
Q

P

A

H

N

Y
x
F

S

A

A

A

K
|
K

A

A

G

G

V

L

L

I

G

G

L

F

A

S

K

K

A

S

M

L

A

A

R

R

E

E

L

V

G

A

P

S

D

R

N

G

V

I

R

T

V

V

N

N

S

V

V

V

T

A

P

P

G

G

L

F

I

I

Q

E

T

T

D

D

I

M

T

T

G

R

G

A

K

-

L

L

T

S

D

D

E

D

L

Q

R

R

T

A

E

G

I

I

L

L

K

A

G

Q

I

V

P

P

L

A

N

G

R

R

L

L

G

G

D

G

A

A

E

Q

D

E

V

I

A

A

R

N

S

A

C

V

L

A

F

F

L

L

A

A

S

S

E

D

L

E

S

A

Y

Y

I

I

T

T

G

G

A

E

T

T

L

L

D

H

V

V

N

N

G

G

M

M

L

Y

I

M

active site: G15 (= G18), S137 (= S140), Q147 (≠ G152), F150 (≠ Y155), K154 (= K159)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (= G12), S13 (≠ A14), R14 (= R17), A35 (≠ I36), T36 (≠ L37), L57 (≠ C60), N58 (≠ D61), V59 (= V62), G87 (= G90), I88 (= I91)

3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
40% identity, 98% coverage: 6:248/249 of query aligns to 5:246/246 of 3osuA

query
sites
3osuA

K

K

V

S

C

A

V

L

I

V

T

T

G

G

A

A

A

S

S

-

R

-

R

R

G
|
G

I

I

G

G

K

R

A

S

T

I

A

A

R

L

L

Q

F

L

A

A

L

E

H

E

G

G

G

Y

R

N

A

V

I

A

I

V

L

N

D

Y

L

A

D

G

G

S

A

K

Q

E

A

K

A

A

E

E

A

A

A

V

E

E

L

E

G

I

E

K

A

A

H

K

-

G

-

V

R

D

G

S

F

F

A

A

-

I

-

Q

C

A

D

N

V

V

T

A

D

D

R

A

D

D

A

E

C

V

F

K

A

A

A

M

A

I

T

K

R

E

V

V

E

S

E

Q

F

F

G

G

R

S

I

L

D

D

V

V

L

L

V

V

N

N

N
|
N

A

A

G

G

I

I

T

T

Q

R

P

D

L

N

K

L

F

L

M

M

D

R

I

M

G

K

P

E

K

Q

N

E

Y

W

E

D

A

D

V

V

T

I

D

D

V

T

S

N

L

L

R

K

G

G

T

V

L

F

Y

N

M

C

S

I

Q

Q

A

K

V

A

V

T

P

P

H

Q

M

M

R

L

E

R

R

Q

K

R

A

S

G

G

S

A

I

I

V

I

C

N

I

L

S

S

S
|
S

V

V

S

V

A

G

Q

A

R

V

G

G

G

N

G

P

I

-

F

-

G

G

G
x
Q

P

A

H

N

Y
|
Y

S

V

A

A

A

T

K
|
K

A

A

G

G

V

V

L

I

G

G

L

L

A

T

K

K

A

S

M

A

A

A

R

R

E

E

L

L

G

A

P

S

D

R

N

G

V

I

R

T

V

V

N

N

S

A

V

V

T

A

P

P

G

G

L

F

I

I

Q

V

T

S

D

D

I

M

T

T

G

D

G

A

K

-

L

L

T

S

D

D

E

E

L

L

R

K

T

E

E

Q

I

M

L

L

K

T

G

Q

I

I

P

P

L

L

N

A

R

R

L

F

G

G

D

Q

A

D

E

T

D

D

V

I

A

A

R

N

S

T

C

V

L

A

F

F

L

L

A

A

S

S

E

D

L

K

S

A

S

K

Y

Y

I

I

T

T

G

G

A

Q

T

T

L

I

D

H

V

V

N

N

G

G

M

M

L

Y

I

M

active site: G15 (= G18), S141 (= S140), Q151 (≠ G152), Y154 (= Y155), K158 (= K159)
binding magnesium ion: N89 (= N88), K158 (= K159)

6t77A Crystal structure of klebsiella pneumoniae fabg(NADPH-dependent) NADP- complex at 1.75 a resolution (see paper)
38% identity, 100% coverage: 1:248/249 of query aligns to 1:243/244 of 6t77A

query
sites
6t77A

M

M

L

S

L

F

S

E

N

G

K

K

V

I

C

A

V

L

I

V

T

T

G
|
G

A

A

A
x
S

S

-

R

-

R
|
R

G
|
G

I

I

G

G

K

R

A

A

T

I

A

A

R

E

L

T

F

L

A

V

L

A

H

R

G

G

G

A

R

K

A

V

I

I

I

-

L

-

D

-

L

-

D

-

G

G

A

T

Q

A

A
x
T

A

S

E

E

A

S

A

G

A

A

E

Q

-

A

-

I

-

S

-

D

-

Y

L

L

G

G

E

A

A

N

H

G

R

K

G

G

F

L

A

M

C
x
L

D
x
N

V
|
V

T

T

D

D

R

P

D

A

A

S

C

I

F

E

A

S

A

V

A

L

T

E

R

N

V

V

V

R

E

A

E

E

F

F

G

G

R

E

I

V

D

D

V

I

L

L

V

V

N

N

A
|
A

G
|
G

I
|
I

T

T

Q

R

P

D

L

N

K

L

F

L

M

M

D

R

I

M

G

K

P

D

K

D

N

E

Y

W

E

N

A

D

V

I

T

I

D

E

V

T

S

N

L

L

R

S

G

S

T

V

L

F

Y

R

M

L

S

S

Q

K

A

A

V

V

V

M

P

R

H

A

M

M

R

M

E

K

R

K

K

R

A

H

G

G

S

R

I

I

V

I

C

T

I

I

S

G

S
|
S

V

V

S

V

A

G

Q

T

R

M

G

G

G

N

G

A

I

-

F

-

G

G

G

Q

P

A

H

N

Y
|
Y

S

A

A

A

K

K

A

A

G

G

V

L

L

I

G

G

L

F

A

S

K

K

A

S

M

L

A

A

R

R

E

E

L

V

G

A

P

S

D

R

N

G

V

I

R

T

V

V

N

N

S

V

V

V

T

A

P

P

G

G

L

F

I

I

Q

E

T

T

D

D

I

M

T

T

G

R

G

A

K

-

L

L

T

T

D

D

E

E

L

Q

R

R

T

A

E

G

I

T

L

L

K

A

G

A

I

V

P

P

L

A

N

G

R

R

L

L

G

G

D

T

A

P

E

N

D

E

V

I

A

A

R

S

S

A

C

V

L

A

F

F

L

L

A

A

S

S

E

D

L

E

S

A

S

S

Y

Y

I

I

T

T

G

G

A

E

T

T

L

L

D

H

V

V

N

N

G

G

M

M

L

Y

I

M

active site: G16 (= G18), S138 (= S140), Y151 (= Y155)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G12), S14 (≠ A14), R15 (= R17), T37 (≠ A44), L58 (≠ C60), N59 (≠ D61), V60 (= V62), A87 (= A89), G88 (= G90), I89 (= I91)

P0A2C9 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
37% identity, 100% coverage: 1:248/249 of query aligns to 1:243/244 of P0A2C9

query
sites
P0A2C9

M

M

L

S

L

F

S

E

N

G

K

K

V

I

C

A

V

L

I

V

T

T

G

G

A

A

A

S

S

-

R

-

R

R

G

G

I

I

G

G

K

R

A

A

T

I

A

A

R

E

L

T

F

L

A

V

L

A

H

R

G

G

G

A

R

K

A

V

I

I

I

G

L

T

D

A

L

T

D

S

G

E

A

N

Q

G

A

A

A

K

E

N

A

I

A

S

A

D

E

Y

L

L

G

G

E

A

A

N

H

G

R

K

G

G

F

L

A

M

C

L

D

N

V

V

T

T

D

D

R

P

D

A

A

S

C

I

F

E

A

S

A

V

A

L

T

E

R

N

V

I

V

R

E

A

E

E

F

F

G

G

R

E

I

V

D

D

V

I

L

L

V

V

N

N

A

A

G

G

I

I

T

T

Q

R

P

D

L

N

K

L

F

L

M

M

D

R

I

M

G

K

P

D

K

D

N

E

Y

W

E

N

A

D

V

I

T

I

D

E

V

T

S

N

L

L

R

S

G

S

T

V

L

F

Y

R

M

L

S

S

Q

K

A

A

V

V

V

M

P

R

H

A

M
|
M

R

M

E

K

R

K

K

R

A

C

G

G

S

R

I

I

V

I

C

T

I

I

S

G

S

S

V

V

S

V

A

G

Q

T

R

M

G

G

G

N

G

A

I

-

F

-

G

G

G

Q

P

A

H

N

Y

Y

S

A

A

A

K

K

A

A

G

G

V

L

L

I

G

G

L

F

A

S

K

K

A

S

M

L

A

A

R

R

E

E

L

V

G

A

P

S

D

R

N

G

V

I

R

T

V

V

N

N

S

V

V

V

T

A

P

P

G

G

L

F

I

I

Q

E

T

T

D

D

I

M

T

T

G

R

G

A

K

-

L

L

T

S

D

D

E

D

L

Q

R

R

T

A

E

G

I

I

L

L

K

A

G

Q

I

V

P

P

L

A

N

G

R

R

L

L

G

G

D

G

A

A

E

Q

D

E

V

I

A

A

R

S

S

A

C

V

L

A

F

F

L

L

A
|
A

S
|
S

E

D

L

E

S

A

S

S

Y

Y

I

I

T

T

G

G

A

E

T

T

L

L

D

H

V

V

N

N

G

G

M

M

L

Y

I

M

M125 (= M127) mutation to I: Loss of the temperature-sensitive phenotype; when associated with T-223.
A223 (= A228) mutation to T: Loss of the temperature-sensitive phenotype; when associated with I-125.
S224 (= S229) mutation to F: Distorts the local conformation and prevent stacking around Phe-221. The S224F mutation would additionally disrupt the hydrogen bond formed between Ser-224 and Glu-226.

3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
39% identity, 98% coverage: 6:248/249 of query aligns to 2:239/239 of 3sj7A

query
sites
3sj7A

K

K

V

S

C

A

V

L

I

V

T

T

G
|
G

A

A

A
x
S

S

-

R

-

R
|
R

G
|
G

I
|
I

G

G

K

R

A

S

T

I

A

A

R

L

L

Q

F

L

A

A

L

E

H

E

G

G

G

Y

R

N

A

V

I

A

I

V

L
x
N

D
x
Y

L
x
A

D
x
G

G
x
S

A

K

Q

E

A

K

A

A

E

E

A

A

A

V

E

E

L

E

G

I

E

K

A

A

H

K

-

G

-

V

R

D

G

S

F

F

A

A

-

I

-

Q

C
x
A

D
x
N

V
|
V

T

A

D

D

R

A

D

D

A

E

C

V

F

K

A

A

A

M

A

I

T

K

R

E

V

V

E

S

E

Q

F

F

G

G

R

S

I

L

D

D

V

V

L

L

V

V

N

N

N
|
N

A
|
A

G

G

I

I

T

T

Q

R

P

D

L

N

K

L

F

L

M

M

D

R

I

M

G

K

P

E

K

Q

N

E

Y

W

E

D

A

D

V

V

T

I

D

D

V
x
T

S

N

L

L

R

K

G

G

T

V

L

F

Y

N

M

C

S

I

Q

Q

A

K

V

A

V

T

P

P

H

Q

M

M

R

L

E

R

R

Q

K

R

A

S

G

G

S

A

I

I

V

I

C

N

I

L

S

S

S
|
S

V

V

S

V

A

G

Q

A

R

V

G

G

G

N

G

P

I

-

F

-

G

G

G
x
Q

P

A

H

N

Y
|
Y

S

V

A

A

A

T

K
|
K

A

A

G

G

V

V

L

I

G

G

L

L

A

T

K

K

A

S

M

A

A

A

R

R

E

E

L

L

G

A

P

S

D

R

N

G

V

I

R

T

V

V

N

N

S

A

V

V

T

A

P
|
P

G
|
G

L

F

I

I

Q

V

T

S

D

D

I

-

T

-

G

-

K

-

L

M

T

T

D

D

E

E

L

L

R

K

T

E

E

Q

I

M

L

L

K

T

G

Q

I

I

P

P

L

L

N

A

R

R

L

F

G

G

D

Q

A

D

E

T

D

D

V

I

A

A

R

N

S

T

C

V

L

A

F

F

L

L

A

A

S

S

E

D

L

K

S

A

S

K

Y

Y

I

I

T

T

G

G

A

Q

T

T

L

I

D

H

V

V

N

N

G

G

M

M

L

Y

I

M

active site: G12 (= G18), S138 (= S140), Q148 (≠ G152), Y151 (= Y155), K155 (= K159)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), S10 (≠ A14), R11 (= R17), I13 (= I19), N31 (≠ L37), Y32 (≠ D38), A33 (≠ L39), G34 (≠ D40), S35 (≠ G41), A58 (≠ C60), N59 (≠ D61), V60 (= V62), N86 (= N88), A87 (= A89), T109 (≠ V111), S138 (= S140), Y151 (= Y155), K155 (= K159), P181 (= P185), G182 (= G186)

P73826 Acetoacetyl-CoA reductase; EC 1.1.1.36 from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa) (see paper)
40% identity, 97% coverage: 3:244/249 of query aligns to 6:236/240 of P73826

query
sites
P73826

L

L

S

E

N

D

K

K

V

V

C

I

V

V

I

V

T

T

G

G

A

G

A

-

S

-

R

N

R

R

G

G

I

I

G

G

K

A

A

A

T

I

A

V

R

K

L

L

F

L

A

Q

L

E

H

M

G

G

G

A

R

K

A

V

I

A

I

F

L

T

D

D

L

L

-

A

-

T

D

D

G

G

A

G

Q

N

A

T

A

-

E

E

A

A

A

L

A

-

E

-

L

-

G

-

E

-

A

-

H

-

R

-

G

G

F

V

A

V

C

A

D

N

V

V

T

T

D

D

R

L

D

E

A

S

C

M

F

T

A

A

T

A

R

E

V

I

V

T

E

D

E

K

F

L

G

G

R

P

I

V

D

Y

V

G

L

V

V

V

N

A

N

N

A

A

G

G

I

I

T

T

Q

K

P

D

L

N

K

F

F

F

M

P

D

K

I

L

G

T

P

P

K

A

N

D

Y

W

E

D

A

A

V

V

T

L

D

N

V

V

S

N

L

L

R

K

G

G

T

V

L

A

Y

Y

M

S

S

I

Q

K

A

P

V

F

V

I

P

E

H

G

M

M

R

Y

E

E

R

R

K

K

A

A

G

G

S

S

I

I

V

V

C

A

I

I

S

S

S
|
S

V

I

S

S

A

G

Q

E

R

R

G

G

G

N

G

-

I

-

F

V

G

G

G

Q

P

T

H

N

Y
|
Y

S

S

A

A

A

T

K
|
K

A

A

G

G

V

V

L

I

G

G

L

M

A

M

K

K

A

S

M

L

A

A

R

R

E

E

L

G

G

A

P

R

D

Y

N

G

V

V

R

R

V

A

N

N

S

A

V

V

T

A

P

P

G

G

L

F

I

I

Q

D

T

T

D

E

I

M

T

T

G

L

G

A

K

-

L

I

T

R

D

E

E

D

L

I

R

R

T

E

E

K

I

I

L

T

K

K

G

E

I

I

P

P

L

F

N

R

R

R

L

F

G

G

D

K

A

P

E

E

D

E

V

I

A

A

R

W

S

A

C

V

L

A

F

F

L

L

A

L

S

S

E

P

L

V

-

A

S

S

Y

Y

I

V

T

T

G

G

A

E

T

V

L

L

D

R

V

V

N

N

G

G

S134 (= S140) mutation to A: 12% enzymatic activity.
Y147 (= Y155) mutation to A: No enzymatic activity.
K151 (= K159) mutation to A: 5% enzymatic activity.

7tzpG Crystal structure of putataive short-chain dehydrogenase/reductase (fabg) from klebsiella pneumoniae subsp. Pneumoniae ntuh-k2044 in complex with nadh (see paper)
38% identity, 100% coverage: 1:248/249 of query aligns to 4:247/247 of 7tzpG

query
sites
7tzpG

M

M

L

K

L

L

S

A

N

S

K

K

V

T

C

A

V

I

I

V

T

T

G
|
G

A

A

S

-

R

-

R
|
R

G
|
G

I
|
I

G

G

K

F

A

G

T

I

A

A

R

Q

L

V

F

L

A

A

L

R

H

E

G

G

G

A

R

R

A

V

I

I

L

A

D
|
D

L
x
R

D

D

-

A

-

H

G

G

A

E

Q

A

A

A

E

A

A

S

A

L

A

R

E

E

L

S

G

G

E

A

A

Q

H

A

R

L

G

F

F

I

A

S

C
|
C

D

N

V
x
I

T

A

D

E

R

K

D

T

A

Q

C

V

F

E

A

A

A

L

A

F

T

S

R

Q

V

A

V

E

E

E

E

A

F

F

G

G

R

P

I

V

D

D

V

I

L

L

V

V

N

N

N
|
N

A

A

G
|
G

I
|
I

T

N

Q

R

P

D

L

A

K

M

F

L

M

H

D

K

I

L

G

T

P

E

K

A

N

D

Y

W

E

D

A

T

V

V

T

I

D

D

V
|
V

S

N

L

L

R

K

G

G

T

T

L

F

Y

L

M

C

S

M

Q

Q

A

Q

V

A

P

I

H

R

M

M

R

R

E

E

R

R

K

G

A

A

G

G

S

R

I

I

V

I

C

N

I

I

S

A

S

S

V

A

S

S

A

W

Q

L

R

-

G

-

G

G

I

N

F

V

G

G

G

Q

P

T

H

N

Y
|
Y

S

S

A

A

A

S

K
|
K

A

A

G

G

V

V

L

V

G

G

L

M

A

T

K

K

A

T

M

A

A

C

R

R

E

E

L

L

G

A

P

K

D

K

N

G

V

V

R

T

V

V

N

N

S

A

V

I

T

C

P

P

G

G

L

F

I
|
I

Q

D

T
|
T

D

D

I

M

T
|
T

G

R

G

G

K

-

L

V

T

P

D

E

E

N

L

V

R

W

T

Q

E

I

I

M

L

V

K

S

G

K

I

I

P

P

L

A

N

G

R

Y

L

A

G

G

D

E

A

A

E

K

D

D

V

V

A

G

R

E

S

C

C

V

L

A

F

F

L

L

A

A

S

S

E

D

L

G

S

A

S

R

Y

Y

I

I

T

N

G

G

A

E

T

V

L

I

D

N

V

V

N

G

G

G

M

M

L

V

I

L

binding 1,4-dihydronicotinamide adenine dinucleotide: G15 (= G12), R18 (= R17), G19 (= G18), I20 (= I19), D39 (= D38), R40 (≠ L39), C63 (= C60), I65 (≠ V62), N91 (= N88), G93 (= G90), I94 (= I91), V114 (= V111), Y155 (= Y155), K159 (= K159), I188 (= I188), T190 (= T190), T193 (= T193)

C1DMX5 L-rhamnose 1-dehydrogenase (NAD(P)(+)); RhaDH; AvLRA1; EC 1.1.1.378 from Azotobacter vinelandii (strain DJ / ATCC BAA-1303) (see paper)
38% identity, 100% coverage: 1:249/249 of query aligns to 1:254/256 of C1DMX5

query
sites
C1DMX5

M

M

L

L

S

I

N

D

K

K

V

T

C

V

V

I

I

V

T

T

G
|
G

A

A

A
x
S

S

-

R

-

R
|
R

G

G

I
|
I

G

G

K

R

A

A

T

A

A

A

R

R

L

E

F

C

A

A

L

R

H

Q

G

G

G

A

R

R

A

V

I

V

I

I

L

G

D

H

L
x
S

D

G

G

S

A

D

Q

E

A

G

A

R

E

A

A

G

A

A

A

L

E

S

L

L

G

A

E

E

A

E

H

I

R

A

G

A

F

F

A

G

-

G

-

T

-

A

-

I

-

A

-

V

-

G

C

A

D
|
D

V
x
A

T

A

D

D

R

L

D

D

A

S

C

G

F

E

A

K

A

L

A

V

T

A

R

A

V

A

V

V

E

E

E

A

F

F

G

G

R

S

I

V

D

D

V

V

L

L

V

V

N

N

N
|
N

A

A

G

G

I

I

T

C

Q

P

P
x
F

L

H

K

S

F

F

M

L

D

D

I

M

G

P

P

R

K

E

N

L

Y

Y

E

L

A

K

V

T

T

V

D

G

V

T

S

N

L

L

R

N

G

G

T

A

L

Y

Y

F

M

T

S

V

Q

Q

A

A

V

A

P

R

H

R

M

M

R

K

E

E

R

Q

-

G

K

R

A

G

G

G

S

A

I

I

V

I

C

A

I

V

S

S

S
|
S

V

I

S
|
S

A

A

Q

L

R

V

G

G

G

A

I

M

F

-

G

-

G
x
Q

P

T

H

H

Y
|
Y

S

T

A

P

A

T

K
|
K

A

A

G

G

V

L

L

L

G

S

L

L

A

M

K

Q

A

S

M

C

A

A

R

I

E

A

L

L

G

G

P

P

D

Y

N

G

V

I

R

R

V

C

N

N

S

A

V

V

T

L

P

P

G

G

L
x
T

I
|
I

Q

A

T

T

D

D

I
|
I

T
x
N

G

K

G

E

K
x
D

L

L

T

S

D

D

-

L

E

E

L

K

R

R

T

E

E

R

I

M

L

T

K

S

G

R

I

V

P

P

L

L

N

G

R

R

L

L

G

G

D

E

A

P

E

D

D

D

V

L

A

A

R

G

S

P

C

I

L

V

F

F

L

L

A

A

S

S

E

D

L

M

S

A

S

R

Y

Y

I

V

T

T

G

G

A

A

T

S

L

L

D

L

V

V

N

D

G

G

M

L

L

F

I

V

H

N

G12 (= G12) binding NADP(+)
S14 (≠ A14) binding NADP(+)
R15 (= R17) binding NADP(+); mutation to T: Increases specificity toward NAD(+). Shows a strong decrease in catalytic efficiency with NADP(+).
I17 (= I19) binding NADP(+)
S37 (≠ L39) binding NADP(+); mutation to H: Increases specificity toward NAD(+). Shows a strong decrease in catalytic efficiency with NADP(+) and an increase in catalytic efficiency with NAD(+).
D66 (= D61) binding NADP(+)
A67 (≠ V62) binding NADP(+)
N93 (= N88) binding NADP(+)
F99 (≠ P94) mutation F->A,Y: Shows a strong decrease in catalytic efficiency with L-rhamnose, L-lyxose and L-mannose.
S146 (= S140) binding beta-L-rhamnose
S148 (= S142) binding beta-L-rhamnose
Q156 (≠ G152) binding beta-L-rhamnose; mutation to A: Almost loss of activity with L-rhamnose as substrate.
Y159 (= Y155) binding beta-L-rhamnose; binding NADP(+)
K163 (= K159) binding NADP(+)
T191 (≠ L187) binding beta-L-rhamnose; mutation to F: Retains 4% of wild-type activity with L-rhamnose as substrate.
I192 (= I188) binding NADP(+)
I196 (= I192) mutation to A: Shows a strong decrease in catalytic efficiency with L-rhamnose as substrate, but does not affect catalytic efficiency with L-lyxose and L-mannose.
N197 (≠ T193) binding beta-L-rhamnose
D200 (≠ K196) mutation to A: Retains 16% of wild-type activity with L-rhamnose as substrate.; mutation to H: Retains 22% of wild-type activity with L-rhamnose as substrate.

3tzcA Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (fabg)(y155f) from vibrio cholerae (see paper)
39% identity, 99% coverage: 1:246/249 of query aligns to 4:223/224 of 3tzcA

query
sites
3tzcA

M

M

L

N

L

L

S

E

N

G

K

K

V

V

C

A

V

L

I

V

T

T

G
|
G

A

A

A
x
S

S

-

R

-

R
|
R

G

G

I

I

G

G

K

K

A

A

T

I

A

A

R

E

L

L

F

L

A

A

L

E

H

R

G

G

G

A

R

K

A

V

I

I

I

-

L

-

D

-

L

-

D

-

G

G

A

T

Q
x
A

A
x
T

A

S

E

E

A

S

A

G

A

A

E

Q

-

A

-

I

-

S

-

D

-

Y

L

L

G

G

E

D

A

N

H

G

R

K

G

G

F

M

A

A

C

L

D
x
N

V
|
V

T

T

D

N

R

P

D

E

A

S

C

I

F

E

A

A

A

V

A

L

T

K

R

A

V

I

V

T

E

D

E

E

F

F

G

G

R

G

I

V

D

D

V

I

L

L

V

V

N

N

A

A

G
|
G

I
|
I

T

T

Q

R

P

-

L

-

K

-

F

-

M

M

D

R

I

M

G

K

P

E

K

E

N

E

Y

W

E

S

A

D

V

I

T

M

D

E

V
x
T

S

N

L

L

R

T

G

S

T

I

L

F

Y

R

M

L

S

S

Q

K

A

A

V

V

V

L

P

R

H

G

M

M

R

M

E

K

R

K

K

R

A

Q

G

G

S

R

I

I

V

I

C

N

I

V

S

G

S

S

V

V

S

-

A

-

Q

-

R

-

G

-

I

-

F

-

G

-

P

-

H

-

Y

-

S

V

A

A

K

K

A

A

G

G

V

V

L

I

G

G

L

F

A

T

K

K

A

S

M

M

A

A

R

R

E

E

L

V

G

A

P

S

D

R

N

G

V

V

R

T

V

V

N

N

S

T

V

V

T

A

P

P

G

G

L

F

I

I

Q

E

T

T

D

D

I

M

T

-

G

-

K

-

L

-

T

-

D

D

E

E

L

Q

R

R

T

T

E

A

I

T

L

L

K

A

G

Q

I

V

P

P

L

A

N

G

R

R

L

L

G

G

D

D

A

P

E

R

D

E

V

I

A

A

R

S

S

A

C

V

L

A

F

F

L

L

A

A

S

S

E

P

L

E

S

A

Y

Y

I

I

T

T

G

G

A

E

T

T

L

L

D

H

V

V

N

N

G

G

M

M

binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G12), S17 (≠ A14), R18 (= R17), A39 (≠ Q43), T40 (≠ A44), N62 (≠ D61), V63 (= V62), G91 (= G90), I92 (= I91), T108 (≠ V111)

4nbtA Crystal structure of fabg from acholeplasma laidlawii (see paper)
39% identity, 98% coverage: 3:246/249 of query aligns to 3:237/239 of 4nbtA

query
sites
4nbtA

L

L

S

E

N

G

K

K

V

V

C

A

V

V

I

I

T

T

G
|
G

A

G

A

A

S

-

R

-

R
x
K

G
|
G

I
x
L

G

G

K

Q

A

A

T

I

A

A

R

L

L

A

F

Y

A

A

L

E

H

E

G

G

G

A

R

K

A

V

I

I

I

A

L

G

D
|
D

L
|
L

D

-

G

-

A

-

Q

-

A

-

E

-

A

-

A

G

E

D

L

L

G

T

E

Y

A

S

H

H

-

P

-

N

-

V

R

E

G

G

F

M

A

Y

C
x
L

D
x
N

V
|
V

T

T

D

D

R

V

D

T

A

G

C

V

F

E

A

K

A

F

A

Y

T

Q

R

S

V

V

V

I

E

D

E

K

F

Y

G

G

R

K

I

I

D

D

V

I

L

L

V

V

N

N

N
|
N

A
|
A

G
|
G

I

I

T

T

Q

K

P

D

L

A

K

M

F

T

M

R

D

K

I

M

G

T

P

E

K

A

N

Q

Y

W

E

D

A

A

V

V

T

I

D

D

V

V

S

N

L

L

R

K

G

G

T

V

L

F

Y

N

M

L

S

T

Q

R

A

L

V

V

V

G

P

P

H

Q

M

M

R

Q

E

T

R

N

K

G

A

Y

G

G

S

S

I

I

V

I

C

N

I
|
I

S

S

S
|
S

V

V

S

V

A

-

Q

-

R

-

G

-

G

G

I

V

F

F

G

G

G

N

-

I

-

G

-

Q

P

A

H

N

Y
|
Y

S

A

A

A

A

T

K
|
K

A

A

G

G

V

V

L

I

G

G

L

L

A

T

K

M

A

T

M

W

A

A

R

K

E

E

-

F

-

A

L

L

G

K

P

G

D

A

N

N

V

V

R

R

V

V

N

N

S

A

V

I

T

A

P
|
P

G
|
G

L

Y

I
|
I

Q

M

T
|
T

D

D

I

I

T

L

G

-

G

K

K

T

L

V

T

P

D

Q

E

D

L

L

R

L

T

D

E

K

I

F

L

A

K

A

G

L

I

T

P

M

L

L

N

N

R

R

L

L

G

G

D

Q

A

P

E

E

D

E

V

I

A

A

R

K

S

V

C

A

L

L

F

F

L

L

A

A

S

S

E

D

L

D

S

A

S

S

Y

Y

I

V

T

T

G

G

A

Q

T

T

L

I

D

N

V

V

N

N

G

G

M

M

active site: G16 (= G18), S132 (= S140), Y145 (= Y155), K149 (= K159)
binding nicotinamide-adenine-dinucleotide: G12 (= G12), K15 (≠ R17), G16 (= G18), L17 (≠ I19), D36 (= D38), L37 (= L39), L52 (≠ C60), N53 (≠ D61), V54 (= V62), N80 (= N88), A81 (= A89), G82 (= G90), I130 (= I138), S132 (= S140), Y145 (= Y155), K149 (= K159), P177 (= P185), G178 (= G186), I180 (= I188), T182 (= T190)

7do7A Crystal structure of azotobacter vinelandii l-rhamnose 1- dehydrogenase(NAD and l-rhamnose bound-form) (see paper)
38% identity, 100% coverage: 2:249/249 of query aligns to 2:254/256 of 7do7A

query
sites
7do7A

L

L

S

I

N

D

K

K

V

T

C

V

V

I

I

V

T

T

G
|
G

A

A

A

S

S

-

R

-

R
|
R

G
|
G

I
|
I

G

G

K

R

A

A

T

A

A

A

R

R

L

E

F

C

A

A

L

R

H

Q

G

G

G

A

R

R

A

V

I

V

I

I

L

G

D

H

L
x
S

D

G

G

S

A

D

Q

E

A

G

A

R

E

A

A

G

A

A

A

L

E

S

L

L

G

A

E

E

A

E

H

I

R

A

G

A

F

F

A

G

-

G

-

T

-

A

-

I

-

A

-

V

-

G

C

A

D
|
D

V
x
A

T

A

D

D

R

L

D

D

A

S

C

G

F

E

A

K

A

L

A

V

T

A

R

A

V

A

V

V

E

E

E

A

F

F

G

G

R

S

I

V

D

D

V

V

L

L

V

V

N

N

N
|
N

A
|
A

G
|
G

I
|
I

T

C

Q

P

P
x
F

L

H

K

S

F

F

M

L

D

D

I

M

G

P

P

R

K

E

N

L

Y

Y

E

L

A

K

V

T

T

V

D

G

V

T

S

N

L

L

R

N

G

G

T

A

L

Y

Y

F

M

T

S

V

Q

Q

A

A

V

A

P

R

H

R

M

M

R

K

E

E

R

Q

-

G

K

R

A

G

G

G

S

A

I

I

V

I

C

A

I
x
V

S
|
S

V

I

S
|
S

A

A

Q

L

R

V

G

G

G

A

I

M

F

-

G

-

G
x
Q

P

T

H

H

Y
|
Y

S

T

A

P

A

T

K
|
K

A

A

G

G

V

L

L

L

G

S

L

L

A

M

K

Q

A

S

M

C

A

A

R

I

E

A

L

L

G

G

P

P

D

Y

N

G

V

I

R

R

V

C

N

N

S

A

V

V

T

L

P
|
P

G
|
G

L

T

I
|
I

Q

A

T
|
T

D

D

I
|
I

T
x
N

G

K

G

E

K

D

L

L

T

S

D

D

-

L

E

E

L

K

R

R

T

E

E

R

I

M

L

T

K

S

G

R

I

V

P

P

L

L

N

G

R

R

L

L

G

G

D

E

A

P

E

D

D

D

V

L

A

A

R

G

S

P

C

I

L

V

F

F

L

L

A

A

S

S

E
x
D

L
x
M

S

A

S
x
R

Y

Y

I

V

T

T

G

G

A

A

T

S

L

L

D

L

V

V

N

D

G

G

M

L

L

F

I

V

H

N

active site: G16 (= G18), S146 (= S140), Y159 (= Y155)
binding nicotinamide-adenine-dinucleotide: G12 (= G12), R15 (= R17), G16 (= G18), I17 (= I19), S37 (≠ L39), D66 (= D61), A67 (≠ V62), N93 (= N88), A94 (= A89), G95 (= G90), I96 (= I91), V144 (≠ I138), S145 (= S139), S146 (= S140), Y159 (= Y155), K163 (= K159), P189 (= P185), G190 (= G186), I192 (= I188), T194 (= T190), I196 (= I192)
binding beta-L-rhamnopyranose: F99 (≠ P94), S146 (= S140), S148 (= S142), Q156 (≠ G152), Y159 (= Y155), N197 (≠ T193), D235 (≠ E230), M236 (≠ L231), R238 (≠ S233)

7b81A Crystal structure of azotobacter vinelandii l-rhamnose 1-dehydrogenase (NAD bound-form) (see paper)
38% identity, 100% coverage: 2:249/249 of query aligns to 2:254/256 of 7b81A

query
sites
7b81A

L

L

S

I

N

D

K

K

V

T

C

V

V

I

I

V

T

T

G
|
G

A

A

A
x
S

S

-

R

-

R
|
R

G
|
G

I
|
I

G

G

K

R

A

A

T

A

A

A

R

R

L

E

F

C

A

A

L

R

H

Q

G

G

G

A

R

R

A

V

I

V

I

I

L

G

D

H

L

S

D

G

G

S

A

D

Q

E

A

G

A

R

E

A

A

G

A

A

A

L

E

S

L

L

G

A

E

E

A

E

H

I

R

A

G

A

F

F

A

G

-

G

-

T

-

A

-

I

-

A

-

V

-

G

C

A

D
|
D

V
x
A

T

A

D

D

R

L

D

D

A

S

C

G

F

E

A

K

A

L

A

V

T

A

R

A

V

A

V

V

E

E

E

A

F

F

G

G

R

S

I

V

D

D

V

V

L

L

V

V

N

N

N
|
N

A
|
A

G
|
G

I
|
I

T

C

Q

P

P

F

L

H

K

S

F

F

M

L

D

D

I

M

G

P

P

R

K

E

N

L

Y

Y

E

L

A

K

V

T

T

V

D

G

V
x
T

S

N

L

L

R

N

G

G

T

A

L

Y

Y

F

M

T

S

V

Q

Q

A

A

V

A

P

R

H

R

M

M

R

K

E

E

R

Q

-

G

K

R

A

G

G

G

S

A

I

I

V

I

C

A

I
x
V

S

S

S
|
S

V

I

S

S

A

A

Q

L

R

V

G

G

G

A

I

M

F

-

G

-

G

Q

P

T

H

H

Y
|
Y

S

T

A

P

A

T

K
|
K

A

A

G

G

V

L

L

L

G

S

L

L

A

M

K

Q

A

S

M

C

A

A

R

I

E

A

L

L

G

G

P

P

D

Y

N

G

V

I

R

R

V

C

N

N

S

A

V

V

T

L

P
|
P

G
|
G

L

T

I
|
I

Q

A

T
|
T

D

D

I
|
I

T

N

G

K

G

E

K

D

L

L

T

S

D

D

-

L

E

E

L

K

R

R

T

E

E

R

I

M

L

T

K

S

G

R

I

V

P

P

L

L

N

G

R

R

L

L

G

G

D

E

A

P

E

D

D

D

V

L

A

A

R

G

S

P

C

I

L

V

F

F

L

L

A

A

S

S

E

D

L

M

S

A

S

R

Y

Y

I

V

T

T

G

G

A

A

T

S

L

L

D

L

V

V

N

D

G

G

M

L

L

F

I

V

H

N

active site: G16 (= G18), S146 (= S140), Y159 (= Y155)
binding nicotinamide-adenine-dinucleotide: G12 (= G12), S14 (≠ A14), R15 (= R17), I17 (= I19), D66 (= D61), A67 (≠ V62), N93 (= N88), A94 (= A89), G95 (= G90), I96 (= I91), T116 (≠ V111), V144 (≠ I138), S146 (= S140), Y159 (= Y155), K163 (= K159), P189 (= P185), G190 (= G186), I192 (= I188), T194 (= T190), I196 (= I192)

Query Sequence

>WP_045585723.1 NCBI__GCF_001305595.1:WP_045585723.1
MLLSNKVCVITGAASRRGIGKATARLFALHGGRAIILDLDGAQAAEAAAELGEAHRGFAC
DVTDRDACFAAATRVVEEFGRIDVLVNNAGITQPLKFMDIGPKNYEAVTDVSLRGTLYMS
QAVVPHMRERKAGSIVCISSVSAQRGGGIFGGPHYSAAKAGVLGLAKAMARELGPDNVRV
NSVTPGLIQTDITGGKLTDELRTEILKGIPLNRLGDAEDVARSCLFLASELSSYITGATL
DVNGGMLIH

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory