SitesBLAST
Comparing WP_046010019.1 NCBI__GCF_000967895.1:WP_046010019.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6rl5G The first crystal structure of the daba aminotransferase ectb in the ectoine biosynthesis pathway of the model organism chromohalobacter salexigens dsm 3034 (see paper)
58% identity, 96% coverage: 2:414/430 of query aligns to 3:418/422 of 6rl5G
- active site: S16 (≠ A15), F137 (= F136), D237 (= D235), K266 (= K264)
- binding pyridoxal-5'-phosphate: G110 (= G109), T111 (= T110), F137 (= F136), H138 (= H137), D237 (= D235), I239 (= I237), Q240 (= Q238), K266 (= K264), G294 (= G292), T295 (= T293)
O66442 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Aquifex aeolicus (strain VF5)
31% identity, 93% coverage: 11:409/430 of query aligns to 5:374/376 of O66442
- GT 96:97 (= GT 109:110) binding pyridoxal 5'-phosphate
- K242 (= K264) modified: N6-(pyridoxal phosphate)lysine
- T271 (= T293) binding pyridoxal 5'-phosphate
2eh6A Crystal structure of acetylornithine aminotransferase from aquifex aeolicus vf5
31% identity, 93% coverage: 11:409/430 of query aligns to 4:373/375 of 2eh6A
- active site: F127 (= F136), E179 (= E202), D212 (= D235), Q215 (= Q238), K241 (= K264), T270 (= T293), R352 (≠ K388)
- binding pyridoxal-5'-phosphate: G95 (= G109), T96 (= T110), F127 (= F136), H128 (= H137), E179 (= E202), D212 (= D235), V214 (≠ I237), K241 (= K264)
1sffA Structure of gamma-aminobutyrate aminotransferase complex with aminooxyacetate (see paper)
29% identity, 92% coverage: 19:413/430 of query aligns to 23:422/425 of 1sffA
- active site: Y137 (≠ F136), E205 (= E202), D238 (= D235), Q241 (= Q238), K267 (= K264), T296 (= T293), R397 (≠ K388)
- binding 4'-deoxy-4'-acetylyamino-pyridoxal-5'-phosphate: Q78 (≠ L74), G110 (= G109), S111 (≠ T110), Y137 (≠ F136), H138 (= H137), R140 (≠ V139), E205 (= E202), D238 (= D235), V240 (≠ I237), Q241 (= Q238), K267 (= K264), T296 (= T293)
- binding sulfate ion: N152 (≠ H151), Y393 (≠ D384)
Sites not aligning to the query:
1sf2A Structure of e. Coli gamma-aminobutyrate aminotransferase (see paper)
29% identity, 92% coverage: 19:413/430 of query aligns to 23:422/425 of 1sf2A
- active site: Y137 (≠ F136), E205 (= E202), D238 (= D235), Q241 (= Q238), K267 (= K264), T296 (= T293), R397 (≠ K388)
- binding pyridoxal-5'-phosphate: G110 (= G109), S111 (≠ T110), Y137 (≠ F136), H138 (= H137), E205 (= E202), D238 (= D235), V240 (≠ I237), Q241 (= Q238), K267 (= K264)
- binding sulfate ion: N152 (≠ H151), Y393 (≠ D384)
Sites not aligning to the query:
P22256 4-aminobutyrate aminotransferase GabT; 5-aminovalerate transaminase; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase; L-AIBAT; EC 2.6.1.19; EC 2.6.1.48 from Escherichia coli (strain K12) (see 2 papers)
29% identity, 92% coverage: 19:413/430 of query aligns to 24:423/426 of P22256
- I50 (≠ A45) mutation to Q: 3-fold decrease in catalytic activity and 12-fold decrease in affinity for GABA.
- GS 111:112 (≠ GT 109:110) binding pyridoxal 5'-phosphate
- E211 (= E207) mutation to S: 100-fold decrease in catalytic activity and 15-fold decrease in affinity for GABA.
- V241 (≠ I237) mutation to A: 25-fold decrease in catalytic activity and 5-fold decrease in affinity for GABA.
- Q242 (= Q238) binding pyridoxal 5'-phosphate
- K268 (= K264) modified: N6-(pyridoxal phosphate)lysine
- T297 (= T293) binding pyridoxal 5'-phosphate
4ppmA Crystal structure of pige: a transaminase involved in the biosynthesis of 2-methyl-3-n-amyl-pyrrole (map) from serratia sp. Fs14 (see paper)
34% identity, 78% coverage: 21:356/430 of query aligns to 47:370/464 of 4ppmA
- active site: Y159 (≠ F136), E212 (= E202), D245 (= D235), Q248 (= Q238), K274 (= K264), T309 (= T293)
- binding magnesium ion: A351 (≠ V336), Y354 (= Y339), V357 (≠ T345)
- binding pyridoxal-5'-phosphate: G132 (= G109), T133 (= T110), Y159 (≠ F136), H160 (= H137), D245 (= D235), V247 (≠ I237), K274 (= K264)
Sites not aligning to the query:
A0A0J9X1Q5 Aminotransferase PigE; EC 2.6.1.- from Serratia sp. (strain FS14) (see paper)
34% identity, 78% coverage: 21:356/430 of query aligns to 418:741/853 of A0A0J9X1Q5
- GT 503:504 (= GT 109:110) binding pyridoxal 5'-phosphate
- K645 (= K264) modified: N6-(pyridoxal phosphate)lysine
- T680 (= T293) binding pyridoxal 5'-phosphate
1szkA The structure of gamma-aminobutyrate aminotransferase mutant: e211s (see paper)
29% identity, 92% coverage: 19:413/430 of query aligns to 23:422/425 of 1szkA
- active site: Y137 (≠ F136), E205 (= E202), D238 (= D235), Q241 (= Q238), K267 (= K264), T296 (= T293), R397 (≠ K388)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G110 (= G109), S111 (≠ T110), Y137 (≠ F136), H138 (= H137), E205 (= E202), D238 (= D235), V240 (≠ I237), Q241 (= Q238), K267 (= K264)
Sites not aligning to the query:
1wkhA Acetylornithine aminotransferase from thermus thermophilus hb8
31% identity, 90% coverage: 24:408/430 of query aligns to 25:383/387 of 1wkhA
- active site: F132 (= F136), E184 (= E202), D217 (= D235), Q220 (= Q238), K246 (= K264), T275 (= T293), R363 (≠ K388)
- binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: Y46 (≠ A45), S104 (≠ T108), G105 (= G109), T106 (= T110), F132 (= F136), S133 (≠ H137), E184 (= E202), E189 (= E207), D217 (= D235), I219 (= I237), K246 (= K264), R363 (≠ K388)
Sites not aligning to the query:
1wkgA Acetylornithine aminotransferase from thermus thermophilus hb8
31% identity, 90% coverage: 24:408/430 of query aligns to 25:383/387 of 1wkgA
- active site: F132 (= F136), E184 (= E202), D217 (= D235), Q220 (= Q238), K246 (= K264), T275 (= T293), R363 (≠ K388)
- binding n~2~-acetyl-n~5~-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-ornithine: Y46 (≠ A45), G105 (= G109), T106 (= T110), F132 (= F136), S133 (≠ H137), R135 (≠ V139), E184 (= E202), D217 (= D235), I219 (= I237), Q220 (= Q238), K246 (= K264), G273 (≠ N291), T274 (≠ G292), T275 (= T293)
Sites not aligning to the query:
1vefA Acetylornithine aminotransferase from thermus thermophilus hb8
31% identity, 90% coverage: 24:408/430 of query aligns to 25:383/387 of 1vefA