SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_046010019.1 NCBI__GCF_000967895.1:WP_046010019.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6rl5G The first crystal structure of the daba aminotransferase ectb in the ectoine biosynthesis pathway of the model organism chromohalobacter salexigens dsm 3034 (see paper)
58% identity, 96% coverage: 2:414/430 of query aligns to 3:418/422 of 6rl5G

query
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6rl5G

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active site: S16 (≠ A15), F137 (= F136), D237 (= D235), K266 (= K264)
binding pyridoxal-5'-phosphate: G110 (= G109), T111 (= T110), F137 (= F136), H138 (= H137), D237 (= D235), I239 (= I237), Q240 (= Q238), K266 (= K264), G294 (= G292), T295 (= T293)

O66442 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Aquifex aeolicus (strain VF5)
31% identity, 93% coverage: 11:409/430 of query aligns to 5:374/376 of O66442

query
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O66442

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GT 96:97 (= GT 109:110) binding pyridoxal 5'-phosphate
K242 (= K264) modified: N6-(pyridoxal phosphate)lysine
T271 (= T293) binding pyridoxal 5'-phosphate

2eh6A Crystal structure of acetylornithine aminotransferase from aquifex aeolicus vf5
31% identity, 93% coverage: 11:409/430 of query aligns to 4:373/375 of 2eh6A

query
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2eh6A

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active site: F127 (= F136), E179 (= E202), D212 (= D235), Q215 (= Q238), K241 (= K264), T270 (= T293), R352 (≠ K388)
binding pyridoxal-5'-phosphate: G95 (= G109), T96 (= T110), F127 (= F136), H128 (= H137), E179 (= E202), D212 (= D235), V214 (≠ I237), K241 (= K264)

P73133 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa) (see paper)
28% identity, 93% coverage: 13:411/430 of query aligns to 38:425/429 of P73133

query
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P73133

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G

A

I

G

A

T

T

L

C

N

T

Y

L

G

G

H

H

N

A

N

H

D

P

H

A

L

L

K

V

A

R

N

A

L

V

M

S

K

D

Y

Q

I

I

M

Q

E

K

D

-

G

-

I

L

T

H

H

H

G

V

L

S

D

N

M

L

H

Y

-

Y

T

I

K

P

A

E

K

Q

G

G

D

E

F

L

L

A

N

K

T

W

F

I

N

V

E

E

K

H

I

S

L

C

K

A

P

D

R

R

D

-

M

-

E

-

Y

-

M

-

V

-

Q

V

F

F

T

F

G

C

P

N

T
x
S

G
|
G

T
x
A

N

E

A

A

V

N

E

E

A

A

M

I

K

K

L

L

A

V

R

R

-

K

-

Y

-

A

-

H

-

T

-

V

-

L

N

D

V

F

T

L

G

E

Q

Q

Q

P

N

V

I

I

V

L

T

T

F

A

T

K

N

A

G

S

F

F

H

H

G

G

V
x
R

S

T

L

L

G

A

A

T

L

I

S

T

A

A

T

T

G

G

N

Q

S

P

H

K

H

Y

R

Q

D

Q

A

Y

A

F

G

D

V

P

S

L

L

V

N

P

G

G

I

F

H

D

R

Y

M

V

P

P

F

Y

D

N

G

-

Y

-

L

-

G

-

D

-

G

-

I

-

D

-

T

D

T

I

E

R

Y

S

L

L

D

E

K

N

V

K

L

V

S

A

D

D

S

L

S

D

S

E

G

G

I

N

N

S

S

R

P

V

A

A

V

I

L

F

L

L

E

E

T

P

V

L

Q

Q

G

G

E
|
E

G

G

V

V

N

R

V

P

A

G

S

D

I

L

E

A

W

Y

L

F

Q

K

N

R

L

V

E

R

K

E

V

I

C

C

R

D

K

Q

H

N

G

D

L

I

L

L

I

V

V

F

D
|
D

D

E

I

V

Q
|
Q

A

V

G

G

C

V

G

G

R

R

T

T

G

G

T

K

F

L

F

W

S

G

F

Y

E

E

E

H

S

L

G

G

I

V

K

E

P

P

D

D

I

I

I

F

T

T

M

S

S

A

K
|
K

S

G

I

L

S

A

G

G

Y

-

G

G

L

V

P

P

F

I

A

G

V

A

V

M

L

M

M

C

K

K

P

K

E

F

L

C

D

D

Q

V

W

F

K

E

P

P

G

G

E

N

H

H

N

A

G

S

T
|
T

F

F

R

G

G

G

N

N

N

P

L

L

A

A

F

C

V

A

T

A

A

G

K

L

D

A

A

V

I

L

D

K

H

T

Y

I

W

E

S

G

D

D

D

R

V

L

F

L

S

D

N

N

E

-

V

V

K

Q

A

A

K

R

G

G

E

E

Y

Q

V

L

K

R

E

S

Q

G

L

L

I

A

D

E

I

I

V

K

G

N

Q

Q

Y

Y

G

P

E

T

G

L

N

F

F

T

T

E

N

V

K

R

G

G

R

W

G

G

M

L

F

I

Q

N

G

G

-

L

-

E

I

I

N

S

C

A

V

E

N

S

G

S

E

L

I

T

A

S

A

V

K

E

I

I

T

V

K

K

K

A

A

A

F

M

S

E

K

Q

G

G

M

L

I

L

E

A

T

P

S

A

G

G

A

-

D

-

D

P

Q

K

I

V

V

L

K
x
R

L

F

F

V

C

P

P

P

L

L

T

V

I

V

T

T

Q

E

E

A

N

E

L

I

E

A

K

Q

G

A

I

V

E

E

I

I

L

L

R

R

E

Q

S

A

I

I

Y39 (= Y14) mutation to F: Retains 7.4% of N-acetylornithine aminotransferase wild-type activity.
S125 (≠ T108) mutation to A: Retains 14% of N-acetylornithine aminotransferase wild-type activity.
G126 (= G109) mutation to A: Loss of N-acetylornithine aminotransferase activity.
A127 (≠ T110) mutation to S: Retains 9.1% of N-acetylornithine aminotransferase wild-type activity.
R163 (≠ V139) mutation to A: 4100-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Retains 9.5% of N-acetylornithine aminotransferase wild-type activity.
E223 (= E207) mutation to A: 65-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Retains 50% of N-acetylornithine aminotransferase wild-type activity.; mutation to S: 73-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Shows 160% of N-acetylornithine aminotransferase wild-type activity.
D251 (= D235) mutation to A: Loss of N-acetylornithine aminotransferase activity.; mutation to E: Retains 20% of N-acetylornithine aminotransferase wild-type activity.
Q254 (= Q238) mutation to A: Retains 0.9% of N-acetylornithine aminotransferase wild-type activity.
K280 (= K264) mutation to A: Loss of N-acetylornithine aminotransferase activity.
T308 (= T293) mutation to A: Retains 0.3% of N-acetylornithine aminotransferase wild-type activity.
R402 (≠ K388) mutation to A: 2080-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Retains 18% of N-acetylornithine aminotransferase wild-type activity.

1sffA Structure of gamma-aminobutyrate aminotransferase complex with aminooxyacetate (see paper)
29% identity, 92% coverage: 19:413/430 of query aligns to 23:422/425 of 1sffA

query
sites
1sffA

P

P

C

I

V

F

F

A

N

D

R

R

A

A

K

E

D

N

E

C

F

R

M

V

W

W

D

D

E

V

E

E

G

G

N

R

R

E

Y

Y

L

L

D

D

F

F

L

A

A

G

G

G

A

I

G

A

T

V

L

L

N

N

Y

T

G

G

H

H

N

L

N

H

D

P

H

K

L

V

K

V

A

A

N

A

L

V

M

E

K

A

Y

Q

I

L

M

K

E

K

D

L

G

S

I

H

T

T

H

C

G

F

L
x
Q

D

V

M

L

H

A

T

Y

K

E

A

P

K

Y

G

L

D

E

F

L

L

C

N

E

T

I

F

M

N

N

E

Q

K

K

I

V

L

-

K

-

P

P

R

G

D

D

M

F

E

A

Y

K

M

K

V

T

Q

L

F

L

T

V

G

-

P

T

T

T

G
|
G

T
x
S

N

E

A

A

V

V

E

E

A

N

A

A

M

V

K

K

L

I

A

A

R

R

N

A

V

A

T

T

G

K

Q

R

Q

S

N

G

I

T

V

I

T

A

F

F

T

S

N

G

G

A

F
x
Y

H
|
H

G

G

V
x
R

S

T

L

H

G

Y

A

T

L

L

S

A

A

L

T

T

G

G

N

K

S

V

H
x
N

H

P

R

Y

D

S

A

A

-

G

-

M

-

G

-

L

-

M

A

P

G

G

V

H

S

V

L

Y

N

R

G

A

I

L

H

Y

R

P

M

C

P

P

F

L

D

H

G

G

Y

I

L

S

G

E

D

D

G

-

I

-

D

D

T

A

T

I

E

A

Y

S

L

I

D

H

K

R

V

I

L

F

S

K

D

N

S

D

S

A

G

P

I

-

N

E

S

D

P

I

A

A

V

I

L

V

L

I

E
|
E

T

P

V

V

Q

Q

G

G

E

E

G

G

V

F

N

Y

V

A

A

S

S

S

I

P

E

A

W

F

L

M

Q

Q

N

R

L

L

E

R

K

A

V

L

C

C

R

D

K

E

H

H

G

G

L

I

L

M

L

L

I

I

V

A

D
|
D

D

E

I
x
V

Q
|
Q

A

S

G

G

C

A

G

G

R

R

T

T

G

G

T

T

F

L

F

F

S

A

F

M

E

E

E

Q

S

M

G

G

I

V

K

A

P

P

D

D

I

L

I

T

T

T

M

F

S

A

K
|
K

S

S

I

I

S

A

G

G

Y

-

G

G

L

F

P

P

F

L

A

A

V

G

V

V

L

T

M

G

K

R

P

A

E

E

-

V

L

M

D

D

Q

A

W

V

K

A

P

P

G

G

E

G

H

L

N

G

G

G

T
|
T

F

Y

R

A

G

G

N

N

N

P

L

I

A

A

F

C

V

V

T

A

A

A

K

L

D

E

A

V

I

L

D

K

H

V

Y

F

W

E

S

Q

D

E

D

N

V

L

F

L

S

-

N

Q

E

K

V

A

K

N

A

D

K

L

G

G

E

Q

Y

K

V

L

K

K

E

D

Q

G

L

L

I

L

D

A

I

I

V

A

G

E

Q

K

Y

H

G

P

E

E

-

I

G

G

N

D

F

V

T

-

N

-

K

R

G

G

R

L

G

G

M

A

F

M

Q

I

G

A

I

I

-

E

-

L

-

F

-

E

-

D

-

G

-

D

-

H

N

N

C

K

V

P

N

D

G

A

E

K

I

L

A

T

A

A

K

E

I

I

T

V

K

A

K

R

A

A

F

R

S

D

K

K

G

G

M

L

I

I

I

L

E

L

T

S

S

C

G

G

A

P

D

Y

D
x
Y

Q

N

I

V

V

L

K
x
R

L

I

F

L

C

V

P

P

L

L

T

T

I

I

T

E

Q

D

E

A

N

Q

L

I

E

R

K

Q

G

G

I

L

E

E

I

I

L

I

R

S

E

Q

S

C

I

F

K

D

E

E

active site: Y137 (≠ F136), E205 (= E202), D238 (= D235), Q241 (= Q238), K267 (= K264), T296 (= T293), R397 (≠ K388)
binding 4'-deoxy-4'-acetylyamino-pyridoxal-5'-phosphate: Q78 (≠ L74), G110 (= G109), S111 (≠ T110), Y137 (≠ F136), H138 (= H137), R140 (≠ V139), E205 (= E202), D238 (= D235), V240 (≠ I237), Q241 (= Q238), K267 (= K264), T296 (= T293)
binding sulfate ion: N152 (≠ H151), Y393 (≠ D384)

Sites not aligning to the query:

active site: 18

1sf2A Structure of e. Coli gamma-aminobutyrate aminotransferase (see paper)
29% identity, 92% coverage: 19:413/430 of query aligns to 23:422/425 of 1sf2A

query
sites
1sf2A

P

P

C

I

V

F

F

A

N

D

R

R

A

A

K

E

D

N

E

C

F

R

M

V

W

W

D

D

E

V

E

E

G

G

N

R

R

E

Y

Y

L

L

D

D

F

F

L

A

A

G

G

G

A

I

G

A

T

V

L

L

N

N

Y

T

G

G

H

H

N

L

N

H

D

P

H

K

L

V

K

V

A

A

N

A

L

V

M

E

K

A

Y

Q

I

L

M

K

E

K

D

L

G

S

I

H

T

T

H

C

G

F

L

Q

D

V

M

L

H

A

T

Y

K

E

A

P

K

Y

G

L

D

E

F

L

L

C

N

E

T

I

F

M

N

N

E

Q

K

K

I

V

L

-

K

-

P

P

R

G

D

D

M

F

E

A

Y

K

M

K

V

T

Q

L

F

L

T

V

G

-

P

T

T

T

G
|
G

T
x
S

N

E

A

A

V

V

E

E

A

N

A

A

M

V

K

K

L

I

A

A

R

R

N

A

V

A

T

T

G

K

Q

R

Q

S

N

G

I

T

V

I

T

A

F

F

T

S

N

G

G

A

F
x
Y

H
|
H

G

G

V

R

S

T

L

H

G

Y

A

T

L

L

S

A

A

L

T

T

G

G

N

K

S

V

H
x
N

H

P

R

Y

D

S

A

A

-

G

-

M

-

G

-

L

-

M

A

P

G

G

V

H

S

V

L

Y

N

R

G

A

I

L

H

Y

R

P

M

C

P

P

F

L

D

H

G

G

Y

I

L

S

G

E

D

D

G

-

I

-

D

D

T

A

T

I

E

A

Y

S

L

I

D

H

K

R

V

I

L

F

S

K

D

N

S

D

S

A

G

P

I

-

N

E

S

D

P

I

A

A

V

I

L

V

L

I

E
|
E

T

P

V

V

Q

Q

G

G

E

E

G

G

V

F

N

Y

V

A

A

S

S

S

I

P

E

A

W

F

L

M

Q

Q

N

R

L

L

E

R

K

A

V

L

C

C

R

D

K

E

H

H

G

G

L

I

L

M

L

L

I

I

V

A

D
|
D

D

E

I
x
V

Q
|
Q

A

S

G

G

C

A

G

G

R

R

T

T

G

G

T

T

F

L

F

F

S

A

F

M

E

E

E

Q

S

M

G

G

I

V

K

A

P

P

D

D

I

L

I

T

T

T

M

F

S

A

K
|
K

S

S

I

I

S

A

G

G

Y

-

G

G

L

F

P

P

F

L

A

A

V

G

V

V

L

T

M

G

K

R

P

A

E

E

-

V

L

M

D

D

Q

A

W

V

K

A

P

P

G

G

E

G

H

L

N

G

G

G

T
|
T

F

Y

R

A

G

G

N

N

N

P

L

I

A

A

F

C

V

V

T

A

A

A

K

L

D

E

A

V

I

L

D

K

H

V

Y

F

W

E

S

Q

D

E

D

N

V

L

F

L

S

-

N

Q

E

K

V

A

K

N

A

D

K

L

G

G

E

Q

Y

K

V

L

K

K

E

D

Q

G

L

L

I

L

D

A

I

I

V

A

G

E

Q

K

Y

H

G

P

E

E

-

I

G

G

N

D

F

V

T

-

N

-

K

R

G

G

R

L

G

G

M

A

F

M

Q

I

G

A

I

I

-

E

-

L

-

F

-

E

-

D

-

G

-

D

-

H

N

N

C

K

V

P

N

D

G

A

E

K

I

L

A

T

A

A

K

E

I

I

T

V

K

A

K

R

A

A

F

R

S

D

K

K

G

G

M

L

I

I

I

L

E

L

T

S

S

C

G

G

A

P

D

Y

D
x
Y

Q

N

I

V

V

L

K
x
R

L

I

F

L

C

V

P

P

L

L

T

T

I

I

T

E

Q

D

E

A

N

Q

L

I

E

R

K

Q

G

G

I

L

E

E

I

I

L

I

R

S

E

Q

S

C

I

F

K

D

E

E

active site: Y137 (≠ F136), E205 (= E202), D238 (= D235), Q241 (= Q238), K267 (= K264), T296 (= T293), R397 (≠ K388)
binding pyridoxal-5'-phosphate: G110 (= G109), S111 (≠ T110), Y137 (≠ F136), H138 (= H137), E205 (= E202), D238 (= D235), V240 (≠ I237), Q241 (= Q238), K267 (= K264)
binding sulfate ion: N152 (≠ H151), Y393 (≠ D384)

Sites not aligning to the query:

active site: 18

P22256 4-aminobutyrate aminotransferase GabT; 5-aminovalerate transaminase; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase; L-AIBAT; EC 2.6.1.19; EC 2.6.1.48 from Escherichia coli (strain K12) (see 2 papers)
29% identity, 92% coverage: 19:413/430 of query aligns to 24:423/426 of P22256

query
sites
P22256

P

P

C

I

V

F

F

A

N

D

R

R

A

A

K

E

D

N

E

C

F

R

M

V

W

W

D

D

E

V

E

E

G

G

N

R

R

E

Y

Y

L

L

D

D

F

F

L

A

A

G

G

G

A
x
I

G

A

T

V

L

L

N

N

Y

T

G

G

H

H

N

L

N

H

D

P

H

K

L

V

K

V

A

A

N

A

L

V

M

E

K

A

Y

Q

I

L

M

K

E

K

D

L

G

S

I

H

T

T

H

C

G

F

L

Q

D

V

M

L

H

A

T

Y

K

E

A

P

K

Y

G

L

D

E

F

L

L

C

N

E

T

I

F

M

N

N

E

Q

K

K

I

V

L

-

K

-

P

P

R

G

D

D

M

F

E

A

Y

K

M

K

V

T

Q

L

F

L

T

V

G

-

P

T

T

T

G
|
G

T
x
S

N

E

A

A

V

V

E

E

A

N

A

A

M

V

K

K

L

I

A

A

R

R

N

A

V

A

T

T

G

K

Q

R

Q

S

N

G

I

T

V

I

T

A

F

F

T

S

N

G

G

A

F

Y

H

H

G

G

V

R

S

T

L

H

G

Y

A

T

L

L

S

A

A

L

T

T

G

G

N

K

S

V

H

N

H

P

R

Y

D

S

A

A

-

G

-

M

-

G

-

L

-

M

A

P

G

G

V

H

S

V

L

Y

N

R

G

A

I

L

H

Y

R

P

M

C

P

P

F

L

D

H

G

G

Y

I

L

S

G

E

D

D

G

-

I

-

D

D

T

A

T

I

E

A

Y

S

L

I

D

H

K

R

V

I

L

F

S

K

D

N

S

D

S

A

G

P

I

-

N

E

S

D

P

I

A

A

V

I

L

V

L

I

E

E

T

P

V

V

Q

Q

G

G

E
|
E

G

G

V

F

N

Y

V

A

A

S

S

S

I

P

E

A

W

F

L

M

Q

Q

N

R

L

L

E

R

K

A

V

L

C

C

R

D

K

E

H

H

G

G

L

I

L

M

L

L

I

I

V

A

D

D

D

E

I
x
V

Q
|
Q

A

S

G

G

C

A

G

G

R

R

T

T

G

G

T

T

F

L

F

F

S

A

F

M

E

E

E

Q

S

M

G

G

I

V

K

A

P

P

D

D

I

L

I

T

T

T

M

F

S

A

K
|
K

S

S

I

I

S

A

G

G

Y

-

G

G

L

F

P

P

F

L

A

A

V

G

V

V

L

T

M

G

K

R

P

A

E

E

-

V

L

M

D

D

Q

A

W

V

K

A

P

P

G

G

E

G

H

L

N

G

G

G

T
|
T

F

Y

R

A

G

G

N

N

N

P

L

I

A

A

F

C

V

V

T

A

A

A

K

L

D

E

A

V

I

L

D

K

H

V

Y

F

W

E

S

Q

D

E

D

N

V

L

F

L

S

-

N

Q

E

K

V

A

K

N

A

D

K

L

G

G

E

Q

Y

K

V

L

K

K

E

D

Q

G

L

L

I

L

D

A

I

I

V

A

G

E

Q

K

Y

H

G

P

E

E

-

I

G

G

N

D

F

V

T

-

N

-

K

R

G

G

R

L

G

G

M

A

F

M

Q

I

G

A

I

I

-

E

-

L

-

F

-

E

-

D

-

G

-

D

-

H

N

N

C

K

V

P

N

D

G

A

E

K

I

L

A

T

A

A

K

E

I

I

T

V

K

A

K

R

A

A

F

R

S

D

K

K

G

G

M

L

I

I

I

L

E

L

T

S

S

C

G

G

A

P

D

Y

Q

N

I

V

V

L

K

R

L

I

F

L

C

V

P

P

L

L

T

T

I

I

T

E

Q

D

E

A

N

Q

L

I

E

R

K

Q

G

G

I

L

E

E

I

I

L

I

R

S

E

Q

S

C

I

F

K

D

E

E

I50 (≠ A45) mutation to Q: 3-fold decrease in catalytic activity and 12-fold decrease in affinity for GABA.
GS 111:112 (≠ GT 109:110) binding pyridoxal 5'-phosphate
E211 (= E207) mutation to S: 100-fold decrease in catalytic activity and 15-fold decrease in affinity for GABA.
V241 (≠ I237) mutation to A: 25-fold decrease in catalytic activity and 5-fold decrease in affinity for GABA.
Q242 (= Q238) binding pyridoxal 5'-phosphate
K268 (= K264) modified: N6-(pyridoxal phosphate)lysine
T297 (= T293) binding pyridoxal 5'-phosphate

4ppmA Crystal structure of pige: a transaminase involved in the biosynthesis of 2-methyl-3-n-amyl-pyrrole (map) from serratia sp. Fs14 (see paper)
34% identity, 78% coverage: 21:356/430 of query aligns to 47:370/464 of 4ppmA

query
sites
4ppmA

V

V

F

F

N

R

R

S

A

A

K

A

D

G

E

T

F

Q

M

L

W

Y

D

D

E

D

E

A

G

G

N

E

R

A

Y

F

L

L

D

D

F

M

L

V

A

A

G

G

A

Y

G

G

T

C

L

L

N

N

Y

L

G

G

H

H

N

N

N

P

D

Q

H

P

L

V

K

V

A

N

N

A

L

L

M

K

K

N

Y

Y

I

L

M

D

E

A

D

Q

G

G

I

P

T

N

H

F

-

I

-

Q

-

Y

-

I

G

S

L

I

D

P

M

E

H

Q

T

T

K

A

A

K

K

L

G

A

D

E

F

V

L

L

N

C

T

R

F

-

N

-

E

-

K

-

I

-

L

L

K

A

P

P

R

G

D

N

M

M

E

G

Y

R

M

V

V

-

Q

-

F

F

T

F

G

S

P

N

T

S

G
|
G

T
|
T

N

E

A

A

V

V

E

E

A

A

M

M

K

K

L

I

A

A

R

K

N

A

V

S

T

T

G

G

Q

K

Q

P

N

G

I

I

V

A

T

Y

F

L

T

R

N

N

G

S

F
x
Y

H
|
H

G

G

V

K

S

T

L

L

G

G

A

A

L

L

S

S

A

I

T

T

G

G

N

R

S

D

H

K

H

H

R

R

D

R

A

Y

A

F

G

T

V

P

S

L

L

L

N

D

G

A

I

M

H

V

R

E

M

V

P

P

F

F

D

-

G

-

Y

-

L

-

G

G

D

D

G

L

I

A

D

A

T

L

T

R

E

E

Y

A

L

L

D

N

K

R

V

-

L

-

S

-

D

-

S

-

G

-

I

-

N

E

S

D

P

V

A

G

A

A

V

L

L

M

L

I

E
|
E

T

P

V

I

Q

Q

G

G

E

E

G

G

V

V

N

H

V

I

A

P

S

P

I

A

E

G

W

Y

L

L

Q

Q

N

A

L

V

E

Q

K

Q

V

L

C

C

R

R

K

E

H

T

G

G

L

V

L

L

I

M

V

V

D
|
D

D

E

I
x
V

Q
|
Q

A

T

G

G

C

L

G

G

R

R

T

T

G

G

T

K

F

L

F

F

S

A

F

C

E

E

E

W

S

D

G

G

I

I

K

E

P

P

D

D

I

V

I

L

T

M

M

L

S

S

K
|
K

S

S

I

L

S

S

G

G

Y

G

G

L

L

I

P

P

F

I

A

G

V

A

V

T

L

L

M

C

K

R

P

A

E

D

L

L

D

W

Q

Q

W

K

K

A

P

Y

G

G

E

T

-

A

-

D

-

R

-

F

-

L

-

V

H

H

N

S

G

S

T
|
T

F

Y

R

G

G

G

N

G

N

N

L

L

A

A

F

S

V

V

T

V

A

A

K

L

D

S

A

A

I

L

D

R

H

E

Y

I

W

L

S

A

D

Q

D

D

V

L

F

V

S

G

N

H

E

A

V

E

K

R

A

-

K

M

G

G

E

A

Y

Y

V

F

K

K

E

Q

Q

A

L

L

I

S

D

E

I

I

V
x
A

G

A

Q

R

Y
|
Y

G

-

E

-

G

-

N

P

F

F

T
x
V

N

S

-

E

-

V

K

R

G

G

R

R

G

G

M

L

F

M

Q

L

G

G

I

I

N

Q

active site: Y159 (≠ F136), E212 (= E202), D245 (= D235), Q248 (= Q238), K274 (= K264), T309 (= T293)
binding magnesium ion: A351 (≠ V336), Y354 (= Y339), V357 (≠ T345)
binding pyridoxal-5'-phosphate: G132 (= G109), T133 (= T110), Y159 (≠ F136), H160 (= H137), D245 (= D235), V247 (≠ I237), K274 (= K264)

Sites not aligning to the query:

active site: 35, 431

A0A0J9X1Q5 Aminotransferase PigE; EC 2.6.1.- from Serratia sp. (strain FS14) (see paper)
34% identity, 78% coverage: 21:356/430 of query aligns to 418:741/853 of A0A0J9X1Q5

query
sites
A0A0J9X1Q5

V

V

F

F

N

R

R

S

A

A

K

A

D

G

E

T

F

Q

M

L

W

Y

D

D

E

D

E

A

G

G

N

E

R

A

Y

F

L

L

D

D

F

M

L

V

A

A

G

G

A

Y

G

G

T

C

L

L

N

N

Y

L

G

G

H

H

N

N

N

P

D

Q

H

P

L

V

K

V

A

N

N

A

L

L

M

K

K

N

Y

Y

I

L

M

D

E

A

D

Q

G

G

I

P

T

N

H

F

-

I

-

Q

-

Y

-

I

G

S

L

I

D

P

M

E

H

Q

T

T

K

A

A

K

K

L

G

A

D

E

F

V

L

L

N

C

T

R

F

-

N

-

E

-

K

-

I

-

L

L

K

A

P

P

R

G

D

N

M

M

E

G

Y

R

M

V

V

-

Q

-

F

F

T

F

G

S

P

N

T

S

G
|
G

T
|
T

N

E

A

A

V

V

E

E

A

A

M

M

K

K

L

I

A

A

R

K

N

A

V

S

T

T

G

G

Q

K

Q

P

N

G

I

I

V

A

T

Y

F

L

T

R

N

N

G

S

F

Y

H

H

G

G

V

K

S

T

L

L

G

G

A

A

L

L

S

S

A

I

T

T

G

G

N

R

S

D

H

K

H

H

R

R

D

R

A

Y

A

F

G

T

V

P

S

L

L

L

N

D

G

A

I

M

H

V

R

E

M

V

P

P

F

F

D

-

G

-

Y

-

L

-

G

G

D

D

G

L

I

A

D

A

T

L

T

R

E

E

Y

A

L

L

D

N

K

R

V

-

L

-

S

-

D

-

S

-

G

-

I

-

N

E

S

D

P

V

A

G

A

A

V

L

L

M

L

I

E

E

T

P

V

I

Q

Q

G

G

E

E

G

G

V

V

N

H

V

I

A

P

S

P

I

A

E

G

W

Y

L

L

Q

Q

N

A

L

V

E

Q

K

Q

V

L

C

C

R

R

K

E

H

T

G

G

L

V

L

L

I

M

V

V

D

D

D

E

I

V

Q

Q

A

T

G

G

C

L

G

G

R

R

T

T

G

G

T

K

F

L

F

F

S

A

F

C

E

E

E

W

S

D

G

G

I

I

K

E

P

P

D

D

I

V

I

L

T

M

M

L

S

S

K
|
K

S

S

I

L

S

S

G

G

Y

G

G

L

L

I

P

P

F

I

A

G

V

A

V

T

L

L

M

C

K

R

P

A

E

D

L

L

D

W

Q

Q

W

K

K

A

P

Y

G

G

E

T

-

A

-

D

-

R

-

F

-

L

-

V

H

H

N

S

G

S

T
|
T

F

Y

R

G

G

G

N

G

N

N

L

L

A

A

F

S

V

V

T

V

A

A

K

L

D

S

A

A

I

L

D

R

H

E

Y

I

W

L

S

A

D

Q

D

D

V

L

F

V

S

G

N

H

E

-

V

A

K

E

A

R

K

M

G

G

E

A

Y

Y

V

F

K

K

E

Q

Q

A

L

L

I

S

D

E

I

I

V

A

G

A

Q

R

Y

Y

G

-

E

-

G

-

N

P

F

F

T

V

N

S

-

E

-

V

K

R

G

G

R

R

G

G

M

L

F

M

Q

L

G

G

I

I

N

Q

GT 503:504 (= GT 109:110) binding pyridoxal 5'-phosphate
K645 (= K264) modified: N6-(pyridoxal phosphate)lysine
T680 (= T293) binding pyridoxal 5'-phosphate

1szkA The structure of gamma-aminobutyrate aminotransferase mutant: e211s (see paper)
29% identity, 92% coverage: 19:413/430 of query aligns to 23:422/425 of 1szkA

query
sites
1szkA

P

P

C

I

V

F

F

A

N

D

R

R

A

A

K

E

D

N

E

C

F

R

M

V

W

W

D

D

E

V

E

E

G

G

N

R

R

E

Y

Y

L

L

D

D

F

F

L

A

A

G

G

G

A

I

G

A

T

V

L

L

N

N

Y

T

G

G

H

H

N

L

N

H

D

P

H

K

L

V

K

V

A

A

N

A

L

V

M

E

K

A

Y

Q

I

L

M

K

E

K

D

L

G

S

I

H

T

T

H

C

G

F

L

Q

D

V

M

L

H

A

T

Y

K

E

A

P

K

Y

G

L

D

E

F

L

L

C

N

E

T

I

F

M

N

N

E

Q

K

K

I

V

L

-

K

-

P

P

R

G

D

D

M

F

E

A

Y

K

M

K

V

T

Q

L

F

L

T

V

G

-

P

T

T

T

G
|
G

T
x
S

N

E

A

A

V

V

E

E

A

N

A

A

M

V

K

K

L

I

A

A

R

R

N

A

V

A

T

T

G

K

Q

R

Q

S

N

G

I

T

V

I

T

A

F

F

T

S

N

G

G

A

F
x
Y

H
|
H

G

G

V

R

S

T

L

H

G

Y

A

T

L

L

S

A

A

L

T

T

G

G

N

K

S

V

H

N

H

P

R

Y

D

S

A

A

-

G

-

M

-

G

-

L

-

M

A

P

G

G

V

H

S

V

L

Y

N

R

G

A

I

L

H

Y

R

P

M

C

P

P

F

L

D

H

G

G

Y

I

L

S

G

E

D

D

G

-

I

-

D

D

T

A

T

I

E

A

Y

S

L

I

D

H

K

R

V

I

L

F

S

K

D

N

S

D

S

A

G

P

I

-

N

E

S

D

P

I

A

A

V

I

L

V

L

I

E
|
E

T

P

V

V

Q

Q

G

G

E

S

G

G

V

F

N

Y

V

A

A

S

S

S

I

P

E

A

W

F

L

M

Q

Q

N

R

L

L

E

R

K

A

V

L

C

C

R

D

K

E

H

H

G

G

L

I

L

M

L

L

I

I

V

A

D
|
D

D

E

I
x
V

Q
|
Q

A

S

G

G

C

A

G

G

R

R

T

T

G

G

T

T

F

L

F

F

S

A

F

M

E

E

E

Q

S

M

G

G

I

V

K

A

P

P

D

D

I

L

I

T

T

T

M

F

S

A

K
|
K

S

S

I

I

S

A

G

G

Y

-

G

G

L

F

P

P

F

L

A

A

V

G

V

V

L

T

M

G

K

R

P

A

E

E

-

V

L

M

D

D

Q

A

W

V

K

A

P

P

G

G

E

G

H

L

N

G

G

G

T
|
T

F

Y

R

A

G

G

N

N

N

P

L

I

A

A

F

C

V

V

T

A

A

A

K

L

D

E

A

V

I

L

D

K

H

V

Y

F

W

E

S

Q

D

E

D

N

V

L

F

L

S

-

N

Q

E

K

V

A

K

N

A

D

K

L

G

G

E

Q

Y

K

V

L

K

K

E

D

Q

G

L

L

I

L

D

A

I

I

V

A

G

E

Q

K

Y

H

G

P

E

E

-

I

G

G

N

D

F

V

T

-

N

-

K

R

G

G

R

L

G

G

M

A

F

M

Q

I

G

A

I

I

-

E

-

L

-

F

-

E

-

D

-

G

-

D

-

H

N

N

C

K

V

P

N

D

G

A

E

K

I

L

A

T

A

A

K

E

I

I

T

V

K

A

K

R

A

A

F

R

S

D

K

K

G

G

M

L

I

I

I

L

E

L

T

S

S

C

G

G

A

P

D

Y

Q

N

I

V

V

L

K
x
R

L

I

F

L

C

V

P

P

L

L

T

T

I

I

T

E

Q

D

E

A

N

Q

L

I

E

R

K

Q

G

G

I

L

E

E

I

I

L

I

R

S

E

Q

S

C

I

F

K

D

E

E

active site: Y137 (≠ F136), E205 (= E202), D238 (= D235), Q241 (= Q238), K267 (= K264), T296 (= T293), R397 (≠ K388)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G110 (= G109), S111 (≠ T110), Y137 (≠ F136), H138 (= H137), E205 (= E202), D238 (= D235), V240 (≠ I237), Q241 (= Q238), K267 (= K264)

Sites not aligning to the query:

active site: 18

1wkhA Acetylornithine aminotransferase from thermus thermophilus hb8
31% identity, 90% coverage: 24:408/430 of query aligns to 25:383/387 of 1wkhA

query
sites
1wkhA

R

R

A

G

K

Q

D

G

E

A

F

R

M

V

W

W

D

D

E

A

E

E

G

G

N

N

R

E

Y

Y

L

I

D

D

F

C

L

V

A

G

G

G

A
x
Y

G

G

T

V

L

A

N

N

Y

L

G

G

H

H

N

G

N

N

D

P

H

E

L

V

K

-

A

V

N

E

L

A

M

V

K

K

Y

R

I

Q

M

A

E

E

D

T

G

L

I

M

T

A

H

M

G

P

L

Q

D

T

M

L

H

P

T

T

K

P

A

M

K

R

G

G

D

E

F

F

L

Y

N

R

T

T

F

L

N

T

E

-

K

A

I

I

L

L

K

P

P

P

R

-

D

-

M

-

E

E

Y

L

M

N

V

R

Q

V

F

F

T

P

G

V

P

N

T
x
S

G
|
G

T
|
T

N

E

A

A

V

N

E

E

A

A

M

L

K

K

L

F

A

A

R

R

N

A

V

H

T

T

G

G

Q

R

Q

K

N

K

I

F

V

V

T

A

F

A

T

M

N

R

G

G

F
|
F

H
x
S

G

G

V

R

S

T

L

M

G

G

A

S

L

L

S

S

A

V

T

T

G

W

N

E

S

P

H

K

H

Y

R

R

D

E

A

P

A

F

G

L

V

P

S

L

L

V

N

E

G

P

I

V

H

E

R

F

M

I

P

P

F

Y

D

N

G

-

Y

-

L

-

G

-

D

-

G

-

I

-

D

-

T

D

T

V

E

E

Y

A

L

L

D

K

K

R

V

A

L

V

S

D

D

E

S

E

S

T

S

-

G

-

I

-

N

-

S

-

P

-

A

A

V

V

L

I

L

L

E
|
E

T

P

V

V

Q

Q

G

G

E
|
E

G

G

V

V

N

R

V

P

A

A

S

T

I

P

E

E

W

F

L

L

Q

R

N

A

L

A

E

R

K

E

V

I

C

T

R

Q

K

E

H

K

G

G

L

A

L

L

I

I

V

L

D
|
D

D

E

I
|
I

Q
|
Q

A

T

G

G

C

M

G

G

R

R

T

T

G

G

T

K

F

R

F

F

S

A

F

F

E

E

E

H

S

F

G

G

I

I

K

V

P

P

D

D

I

I

I

L

T

T

M

L

S

A

K
|
K

S

A

I

L

S

G

G

G

Y

-

G

G

L

V

P

P

F

L

A

G

V

V

V

A

L

V

M

M

K

R

P

E

E

E

L

V

D

A

Q

R

W

S

K

M

P

P

-

K

G

G

E

G

H

H

N

G

G

T

T
|
T

F

F

R

G

G

G

N

N

N

P

L

L

A

A

F

M

V

A

T

A

A

G

K

V

D

A

A

A

I

I

D

R

H

Y

Y

L

W

E

S

R

D

T

D

R

V

L

F

W

S

E

N

R

E

A

V

A

K

E

A

L

K

-

G

G

E

P

Y

W

V

F

K

M

E

E

Q

K

L

L

I

R

D

A

I

I

V

P

G

S

Q

P

Y

K

G

-

E

-

G

-

N

-

F

-

T

I

N

R

K

E

G

V

R

R

G

G

M

M

F

G

Q

L

G

M

I

V

N

G

C

L

V

E

N

L

G

K

E

E

I

K

A

A

K

P

I

Y

T

I

K

A

K

R

A

L

F

E

S

K

K

E

G

H

M

R

I

V

I

L

E

A

T

L

S

Q

G

-

A

A

D

G

D

P

Q

T

I

V

V

I

K
x
R

L

F

F

L

C

P

P

P

L

L

T

V

I

I

T

E

Q

K

E

E

N

D

L

L

E

E

K

R

G

V

I

V

E

E

I

A

L

V

R

R

active site: F132 (= F136), E184 (= E202), D217 (= D235), Q220 (= Q238), K246 (= K264), T275 (= T293), R363 (≠ K388)
binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: Y46 (≠ A45), S104 (≠ T108), G105 (= G109), T106 (= T110), F132 (= F136), S133 (≠ H137), E184 (= E202), E189 (= E207), D217 (= D235), I219 (= I237), K246 (= K264), R363 (≠ K388)

Sites not aligning to the query:

active site: 13

1wkgA Acetylornithine aminotransferase from thermus thermophilus hb8
31% identity, 90% coverage: 24:408/430 of query aligns to 25:383/387 of 1wkgA

query
sites
1wkgA

R

R

A

G

K

Q

D

G

E

A

F

R

M

V

W

W

D

D

E

A

E

E

G

G

N

N

R

E

Y

Y

L

I

D

D

F

C

L

V

A

G

G

G

A
x
Y

G

G

T

V

L

A

N

N

Y

L

G

G

H

H

N

G

N

N

D

P

H

E

L

V

K

-

A

V

N

E

L

A

M

V

K

K

Y

R

I

Q

M

A

E

E

D

T

G

L

I

M

T

A

H

M

G

P

L

Q

D

T

M

L

H

P

T

T

K

P

A

M

K

R

G

G

D

E

F

F

L

Y

N

R

T

T

F

L

N

T

E

-

K

A

I

I

L

L

K

P

P

P

R

-

D

-

M

-

E

E

Y

L

M

N

V

R

Q

V

F

F

T

P

G

V

P

N

T

S

G
|
G

T
|
T

N

E

A

A

V

N

E

E

A

A

M

L

K

K

L

F

A

A

R

R

N

A

V

H

T

T

G

G

Q

R

Q

K

N

K

I

F

V

V

T

A

F

A

T

M

N

R

G

G

F
|
F

H
x
S

G

G

V
x
R

S

T

L

M

G

G

A

S

L

L

S

S

A

V

T

T

G

W

N

E

S

P

H

K

H

Y

R

R

D

E

A

P

A

F

G

L

V

P

S

L

L

V

N

E

G

P

I

V

H

E

R

F

M

I

P

P

F

Y

D

N

G

-

Y

-

L

-

G

-

D

-

G

-

I

-

D

-

T

D

T

V

E

E

Y

A

L

L

D

K

K

R

V

A

L

V

S

D

D

E

S

E

S

T

S

-

G

-

I

-

N

-

S

-

P

-

A

A

V

V

L

I

L

L

E
|
E

T

P

V

V

Q

Q

G

G

E

E

G

G

V

V

N

R

V

P

A

A

S

T

I

P

E

E

W

F

L

L

Q

R

N

A

L

A

E

R

K

E

V

I

C

T

R

Q

K

E

H

K

G

G

L

A

L

L

I

I

V

L

D
|
D

D

E

I
|
I

Q
|
Q

A

T

G

G

C

M

G

G

R

R

T

T

G

G

T

K

F

R

F

F

S

A

F

F

E

E

E

H

S

F

G

G

I

I

K

V

P

P

D

D

I

I

I

L

T

T

M

L

S

A

K
|
K

S

A

I

L

S

G

G

G

Y

-

G

G

L

V

P

P

F

L

A

G

V

V

V

A

L

V

M

M

K

R

P

E

E

E

L

V

D

A

Q

R

W

S

K

M

P

P

-

K

G

G

E

G

H

H

N
x
G

G
x
T

T
|
T

F

F

R

G

G

G

N

N

N

P

L

L

A

A

F

M

V

A

T

A

A

G

K

V

D

A

A

A

I

I

D

R

H

Y

Y

L

W

E

S

R

D

T

D

R

V

L

F

W

S

E

N

R

E

A

V

A

K

E

A

L

K

-

G

G

E

P

Y

W

V

F

K

M

E

E

Q

K

L

L

I

R

D

A

I

I

V

P

G

S

Q

P

Y

K

G

-

E

-

G

-

N

-

F

-

T

I

N

R

K

E

G

V

R

R

G

G

M

M

F

G

Q

L

G

M

I

V

N

G

C

L

V

E

N

L

G

K

E

E

I

K

A

A

K

P

I

Y

T

I

K

A

K

R

A

L

F

E

S

K

K

E

G

H

M

R

I

V

I

L

E

A

T

L

S

Q

G

-

A

A

D

G

D

P

Q

T

I

V

V

I

K
x
R

L

F

F

L

C

P

P

P

L

L

T

V

I

I

T

E

Q

K

E

E

N

D

L

L

E

E

K

R

G

V

I

V

E

E

I

A

L

V

R

R

active site: F132 (= F136), E184 (= E202), D217 (= D235), Q220 (= Q238), K246 (= K264), T275 (= T293), R363 (≠ K388)
binding n~2~-acetyl-n~5~-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-ornithine: Y46 (≠ A45), G105 (= G109), T106 (= T110), F132 (= F136), S133 (≠ H137), R135 (≠ V139), E184 (= E202), D217 (= D235), I219 (= I237), Q220 (= Q238), K246 (= K264), G273 (≠ N291), T274 (≠ G292), T275 (= T293)

Sites not aligning to the query:

1vefA Acetylornithine aminotransferase from thermus thermophilus hb8
31% identity, 90% coverage: 24:408/430 of query aligns to 25:383/387 of 1vefA

query
sites
1vefA

R

R

A

G

K

Q

D

G

E

A

F

R

M

V

W

W

D

D

E

A

E

E

G

G

N

N

R

E

Y

Y

L

I

D

D

F

C

L

V

A

G

G

G

A

Y

G

G

T

V

L

A

N

N

Y

L

G

G

H

H

N

G

N

N

D

P

H

E

L

V

K

-

A

V

N

E

L

A

M

V

K

K

Y

R

I

Q

M

A

E

E

D

T

G

L

I

M

T

A

H

M

G

P

L

Q

D

T

M

L

H

P

T

T

K

P

A

M

K

R

G

G

D

E

F

F

L

Y

N

R

T

T

F

L

N

T

E

-

K

A

I

I

L

L

K

P

P

P

R

-

D

-

M

-

E

E

Y

L

M

N

V

R

Q

V

F

F

T

P

G

V

P

N

T

S

G
|
G

T
|
T

N

E

A

A

V

N

E

E

A

A

M

L

K

K

L

F

A

A

R

R

N

A

V

H

T

T

G

G

Q

R

Q

K

N

K

I

F

V

V

T

A

F

A

T

M

N

R

G

G

F
|
F

H
x
S

G

G

V

R

S

T

L

M

G

G

A

S

L

L

S

S

A

V

T

T

G

W

N

E

S

P

H

K

H

Y

R

R

D

E

A

P

A

F

G

L

V

P

S

L

L

V

N

E

G

P

I

V

H

E

R

F

M

I

P

P

F

Y

D

N

G

-

Y

-

L

-

G

-

D

-

G

-

I

-

D

-

T

D

T

V

E

E

Y

A

L

L

D

K

K

R

V

A

L

V

S

D

D

E

S

E

S

T

S

-

G

-

I

-

N

-

S

-

P

-

A

A

V

V

L

I

L

L

E
|
E

T

P

V

V

Q

Q

G

G

E

E

G

G

V

V

N

R

V

P

A

A

S

T

I

P

E

E

W

F

L

L

Q

R

N

A

L

A

E

R

K

E

V

I

C

T

R

Q

K

E

H

K

G

G

L

A

L

L

I

I

V

L

D
|
D

D

E

I
|
I

Q

Q

A

T

G

G

C

M

G

G

R

R

T

T

G

G

T

K

F

R

F

F

S

A

F

F

E

E

E

H

S

F

G

G

I

I

K

V

P

P

D

D

I

I

I

L

T

T

M

L

S

A

K
|
K

S

A

I

L

S

G

G

G

Y

-

G

G

L

V

P

P

F

L

A

G

V

V

V

A

L

V

M

M

K

R

P

E

E

E

L

V

D

A

Q

R

W

S

K

M

P

P

-

K

G

G

E

G

H

H

N

G

G

T

T
|
T

F

F

R

G

G

G

N

N

N

P

L

L

A

A

F

M

V

A

T

A

A

G

K

V

D

A

A

A

I

I

D

R

H

Y

Y

L

W

E

S

R

D

T

D

R

V

L

F

W

S

E

N

R

E

A

V

A

K

E

A

L

K

-

G

G

E

P

Y

W

V

F

K

M

E

E

Q

K

L

L

I

R

D

A

I

I

V

P

G

S

Q

P

Y

K

G

-

E

-

G

-

N

-

F

-

T

I

N

R

K

E

G

V

R

R

G

G

M

M

F

G

Q

L

G

M

I

V

N

G

C

L

V

E

N

L

G

K

E

E

I

K

A

A

K

P

I

Y

T

I

K

A

K

R

A

L

F

E

S

K

K

E

G

H

M

R

I

V

I

L

E

A

T

L

S

Q

G

-

A

A

D

G

D

P

Q

T

I

V

V

I

K
x
R

L

F

F

L

C

P

P

P

L

L

T

V

I

I

T

E

Q

K

E

E

N

D

L

L

E

E

K

R

G

V

I

V

E

E

I

A

L

V

R

R

active site: F132 (= F136), D217 (= D235), K246 (= K264), T275 (= T293), R363 (≠ K388)
binding pyridoxal-5'-phosphate: G105 (= G109), T106 (= T110), F132 (= F136), S133 (≠ H137), E184 (= E202), D217 (= D235), I219 (= I237), K246 (= K264)

Sites not aligning to the query:

active site: 13

8ht4B Crystal structure of acetylornithine aminotransferase complex with plp from corynebacterium glutamicum (see paper)
30% identity, 91% coverage: 19:411/430 of query aligns to 18:389/390 of 8ht4B

query
sites
8ht4B

P

P

C

V

V

E

F

L

N

V

R

S

A

G

K

K

D

G

E

A

F

T

M

V

W

T

D

D

E

D

E

Q

G

G

N

N

R

V

Y

Y

L

I

D

D

F

L

L

L

A

A

G

G

A

I

G

A

T

V

L

N

N

A

Y

L

G

G

H

H

N

A

N

H

D

P

H

A

L

I

K

I

A

E

N

A

L

V

M

T

K

N

Y

Q

I

I

M

G

E

Q

D

L

G

G

I

H

T

V

H

S

G

N

L

L

D

F

M

A

H

S

-

R

-

P

-

V

T

V

K

E

A

V

K

A

G

E

D

E

F

L

L

I

N

K

T

R

F

F

-

S

-

L

N

D

E

D

K

A

I

T

L

L

K

A

P

A

R

Q

D

T

M

R

E

V

Y

F

M

F

V

C

Q

N

F

-

T

-

G

-

P

-

T
x
S

G
|
G

T
x
A

N

E

A

A

V

N

E

E

A

A

M

F

K

K

L

I

A

A

R

R

N

-

V

L

T

T

G

G

Q

R

Q

S

N

R

I

I

V

L

T

A

F

A

T

V

N

H

G

G

F
|
F

H
|
H

G

G

V

R

S

T

L

M

G

G

A

S

L

L

S

A

A

L

T

T

G

G

N

Q

S

P

H

D

H

K

R

R

D

E

A

A

A

F

G

L

V

P

S

M

L

P

N

S

G

G

I

V

H

E

R

F

M

Y

P

P

F

Y

D

-

G

-

Y

-

L

-

G

G

D

D

G

-

I

-

D

-

T

-

T

T

E

D

Y

Y

L

L

D

R

K

K

V

M

L

V

S

E

D

T

S

N

S

P

S

T

G

D

I

V

N

-

S

-

P

-

A

A

V

I

L

F

L

L

E
|
E

T

P

V

I

Q

Q

G

G

E

E

G

T

G

G

V

V

N

V

V

P

A

A

S

P

I

E

E

G

W

F

L

L

Q

K

N

A

L

V

E

R

K

E

V

L

C

C

R

D

K

E

H

Y

G

G

L

I

L

L

L

M

I

I

V

T

D
|
D

D

E

I
x
V

Q
|
Q

A

T

G

G

C

V

G

G

R

R

T

T

G

G

T

D

F

F

S

A

F

H

E

Q

E

H

S

D

G

G

I

V

K

V

P

P

D

D

I

V

T

T

M

M

S

A

K
|
K

S

G

I

L

S

G

G

G

Y

-

G

G

L

L

P

P

F

I

A

G

V

A

V

C

L

L

M

A

K

T

P

G

E

R

L

A

D

A

Q

E

W

L

-

M

K

T

P

P

G

G

E

K

H

H

N

G

G

T

T

T

F

F

R

G

G

G

N

N

N

P

L

V

A

A

F

C

V

A

T

A

A

A

K

K

D

A

A

V

I

L

D

S

H

-

Y

-

W

V

S

V

D

D

V

A

F

F

S

C

N

A

E

E

V

V

K

A

A

R

K

K

G

G

E

E

Y

L

V

F

K

K

E

E

Q

L

L

L

I

A

D

K

I

V

V

-

G

-

Q

-

Y

-

G

-

E

D

G

G

N

V

F

V

T

D

N

V

K

R

G

G

R

R

G

G

M

L

F

M

Q

L

G

G

I

V

N

-

C

V

V

L

N

E

G

R

E

D

I

V

A

A

A

K

K

Q

I

A

T

V

K

L

K

D

A

G

F

F

S

K

K

H

G

G

M

V

I

I

I

L

E

N

T

A

S

P

G

A

A

-

D

-

D

D

Q

N

I

I

V

I

K

R

L

L

F

T

C

P

P

P

L

L

T

V

I

I

T

T

Q

D

E

E

N

E

L

I

E

A

K

D

G

A

I

V

E

K

I

A

L

I

R

A

E

E

S

T

I

I

binding pyridoxal-5'-phosphate: S108 (≠ T108), G109 (= G109), A110 (≠ T110), F135 (= F136), H136 (= H137), E190 (= E202), D223 (= D235), V225 (≠ I237), Q226 (= Q238), K252 (= K264)

Q5SHH5 [LysW]-aminoadipate semialdehyde transaminase; EC 2.6.1.118 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
31% identity, 90% coverage: 24:408/430 of query aligns to 33:391/395 of Q5SHH5

query
sites
Q5SHH5

R

R

A

G

K

Q

D

G

E

A

F

R

M

V

W

W

D

D

E

A

E

E

G

G

N

N

R

E

Y

Y

L

I

D

D

F

C

L

V

A

G

G

G

A

Y

G

G

T

V

L

A

N

N

Y

L

G

G

H

H

N

G

N

N

D

P

H

E

L

V

K

-

A

V

N

E

L

A

M

V

K

K

Y

R

I

Q

M

A

E

E

D

T

G

L

I

M

T

A

H

M

G

P

L

Q

D

T

M

L

H

P

T

T

K

P

A

M

K

R

G

G

D

E

F

F

L

Y

N

R

T

T

F

L

N

T

E

-

K

A

I

I

L

L

K

P

P

P

R

-

D

-

M

-

E

E

Y

L

M

N

V

R

Q

V

F

F

T

P

G

V

P

N

T

S

G
|
G

T
|
T

N

E

A

A

V

N

E

E

A

A

M

L

K

K

L

F

A

A

R

R

N

A

V

H

T

T

G

G

Q

R

Q

K

N

K

I

F

V

V

T

A

F

A

T

M

N

R

G

G

F

F

H

S

G

G

V

R

S

T

L

M

G

G

A

S

L

L

S

S

A

V

T

T

G

W

N

E

S

P

H

K

H

Y

R

R

D

E

A

P

A

F

G

L

V

P

S

L

L

V

N

E

G

P

I

V

H

E

R

F

M

I

P

P

F

Y

D

N

G

-

Y

-

L

-

G

-

D

-

G

-

I

-

D

-

T

D

T

V

E

E

Y

A

L

L

D

K

K

R

V

A

L

V

S

D

D

E

S

E

S

T

S

-

G

-

I

-

N

-

S

-

P

-

A

A

V

V

L

I

L

L

E

E

T

P

V

V

Q

Q

G

G

E

E

G

G

V

V

N

R

V

P

A

A

S

T

I

P

E

E

W

F

L

L

Q

R

N

A

L

A

E

R

K

E

V

I

C

T

R

Q

K

E

H

K

G

G

L

A

L

L

I

I

V

L

D

D

D

E

I

I

Q

Q

A

T

G

G

C

M

G

G

R

R

T

T

G

G

T

K

F

R

F

F

S

A

F

F

E

E

E

H

S

F

G

G

I

I

K

V

P

P

D

D

I

I

I

L

T

T

M

L

S

A

K
|
K

S

A

I

L

S

G

G

G

Y

-

G

G

L

V

P

P

F

L

A

G

V

A

L

V

M

M

K

R

P

E

E

E

L

V

D

A

Q

R

W

S

K

M

P

P

-

K

G

G

E

G

H

H

N

G

G

T

T
|
T

F

F

R

G

G

G

N

N

N

P

L

L

A

A

F

M

V

A

T

A

A

G

K

V

D

A

A

A

I

I

D

R

H

Y

Y

L

W

E

S

R

D

T

D

R

V

L

F

W

S

E

N

R

E

A

V

A

K

E

A

L

K

-

G

G

E

P

Y

W

V

F

K

M

E

E

Q

K

L

L

I

R

D

A

I

I

V

P

G

S

Q

P

Y

K

G

-

E

-

G

-

N

-

F

-

T

I

N

R

K

E

G

V

R

R

G

G

M

M

F

G

Q

L

G

M

I

V

N

G

C

L

V

E

N

L

G

K

E

E

I

K

A

A

K

P

I

Y

T

I

K

A

K

R

A

L

F

E

S

K

K

E

G

H

M

R

I

V

I

L

E

A

T

L

S

Q

G

-

A

A

D

G

D

P

Q

T

I

V

V

I

K

R

L

F

F

L

C

P

P

P

L

L

T

V

I

I

T

E

Q

K

E

E

N

D

L

L

E

E

K

R

G

V

I

V

E

E

I

A

L

V

R

R

GT 113:114 (= GT 109:110) binding pyridoxal 5'-phosphate
K254 (= K264) modified: N6-(pyridoxal phosphate)lysine
T283 (= T293) binding pyridoxal 5'-phosphate

P50457 4-aminobutyrate aminotransferase PuuE; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase; EC 2.6.1.19 from Escherichia coli (strain K12) (see paper)
30% identity, 90% coverage: 25:413/430 of query aligns to 29:421/421 of P50457

query
sites
P50457

A

A

K

E

D

N

E

A

F

T

M

L

W

K

D

D

E

V

E

E

G

G

N

N

R

E

Y

Y

L

I

D

D

F

F

L

A

A

A

G

G

A

I

G

A

T

V

L

L

N

N

Y

T

G

G

H

H

N

R

N

H

D

P

H

D

L

L

K

V

A

A

N

A

L

V

M

E

K

Q

Y

Q

I

L

M

Q

E

Q

D

-

G

-

I

F

T

T

H

H

G

-

L

-

D

T

M

A

H

Y

T

Q

K

I

A

V

K

P

G

Y

D

E

F

S

L

Y

N

V

T

T

F

L

N

A

E

E

K

K

I

I

-

N

-

A

L

L

K

A

P

P

R

V

D

S

M

G

E

Q

Y

A

M

K

V

T

Q

A

F

F

T

F

G

T

P

-

T

T

G

G

T

A

N

E

A

A

V

V

E

E

A

N

A

A

M

V

K

K

L

I

A

A

R

R

N

A

V

H

T

T

G

G

Q

R

Q

P

N

G

I

V

V

I

T

A

F

F

T

S

N

G

G

G

F

F

H

H

G

G

V

R

S

T

L

Y

G

M

A

T

L

M

S

A

A

L

T

T

G

G

N

K

-

V

S

A

H

P

H

Y

R

K

D

I

A

G

A

F

G

G

V

P

S

F

L

P

N

G

G

S

I

V

H

Y

R

H

M

V

P

P

F

Y

D

P

G

S

Y

D

L

L

G

-

D

H

G

G

I

I

D

S

T

T

T

Q

E

D

Y

S

L

L

D

D

K

A

V

I

L

E

S

R

D

L

S

F

S

K

S

S

G

D

I

I

N

E

S

A

P

K

-

Q

-

V

A

A

V

I

L

I

L

F

E

E

T

P

V

V

Q

Q

G

G

E

E

G

G

V

F

N

N

V

V

A

A

S

P

I

K

E

E

W

L

L

V

Q

A

N

A

L

I

E

R

K

R

V

L

C

C

R

D

K

E

H

H

G

G

L

I

L

V

L

M

I

I

V

A

D

D

D

E

I

V

Q

Q

A

S

G

G

C

F

G

A

R

R

T

T

G

G

T

K

F

L

F

F

S

A

F

M

E

D

E

H

S

Y

G

A

I

D

K

K

P

P

D

D

I

L

I

M

T

T

M

M

S

A

K
|
K

S

S

I

L

S

A

G

G

Y

-

G

G

L

M

P

P

F

L

A

S

V

G

V

V

L

V

M

G

K

N

P

A

E

N

-

I

L

M

D

D

Q

A

W

P

K

A

P

P

G

G

E

G

H

L

N

G

G

G

T

T

F

Y

R

A

G

G

N

N

N

P

L

L

A

A

F

V

V

A

T

A

A

A

K

H

D

A

A

V

I

L

D

N

H

-

Y

I

W

I

S

D

D

K

D

E

V

S

F

L

S

C

N

E

E

R

V

A

K

N

A

Q

K

L

G

G

E

Q

Y

R

V

L

K

K

E

N

Q

T

L

L

I

I

D

D

-

A

-

K

-

E

-

S

-

V

-

P

-

A

I

I

V

A

G

A

Q

V

Y

R

G

G

E

L

G

G

N

S

-

M

-

I

-

A

-

V

-

E

F

F

T

N

N

D

K

P

G

Q

R

T

G

G

M

E

F

-

Q

-

G

-

I

-

N

-

C

-

V

P

N

S

G

A

E

A

I

I

A

A

A

Q

K

K

I

I

T

Q

K

Q

K

R

A

A

F

L

S

A

K

Q

G

G

M

L

I

L

E

L

T

T

S

C

G

G

A

A

D

Y

D

G

Q

N

I

V

V

I

K

R

L

F

F

L

C

Y

P

P

L

L

T

T

I

I

T

P

Q

D

E

A

N

Q

L

F

E

D

K

A

G

A

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M

E

K

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L

L

R

Q

E

D

S

A

I

L

K

S

E

D

K267 (= K264) mutation to A: No GABA-AT activity.

P42588 Putrescine aminotransferase; PAT; PATase; Cadaverine transaminase; Diamine transaminase; Putrescine transaminase; Putrescine--2-oxoglutaric acid transaminase; Putrescine:2-OG aminotransferase; EC 2.6.1.82; EC 2.6.1.29 from Escherichia coli (strain K12) (see paper)
28% identity, 89% coverage: 30:411/430 of query aligns to 76:449/459 of P42588

query
sites
P42588

M

L

W

V

D

D

E

T

E

Q

G

G

N

Q

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E

Y

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L

I

D

D

F

C

L

L

A

G

G

G

A

F

G

G

T

I

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F

N

N

Y

V

G

G

H

H

N

R

N

N

D

P

H

V

L

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K

V

A

S

N

A

L

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M

Q

K

N

Y

Q

I

L

M

A

E

K

D

Q

G

-

I

-

T

-

H

-

G

-

L

-

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P

M

L

H

H

T

S

K

Q

A

E

K

L

G

L

D

D

F

P

L

L

N

R

T

A

F

M

N

L

E

A

K

K

I

T

L

L

K

A

-

A

-

L

-

T

P

P

R

G

D

K

M

L

E

K

Y

Y

M

-

V

-

Q

S

F

F

T

F

G

C

P

N

T

S

G
|
G

T
|
T

N

E

A

S

V

V

E

E

A

A

M

L

K

K

L

L

A

A

R

K

N

A

V

Y

T

Q

G

S

Q

P

Q

R

N

G

I

K

V

F

T

T

F

F

-

I

-

A

-

T

T

S

N

G

G

A

F

F

H

H

G

G

V

K

S

S

L

L

G

G

A

A

L

L

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S

A

A

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T

G

A

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K

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S

H

T

H

F

R

R

D

K

A

P

A

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M

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S

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L

L

N

P

G

G

I

F

H

R

R

H

M

V

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P

F

F

D

G

G

-

Y

-

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-

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-

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-

G

-

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-

D

-

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N

T

I

E

E

Y

A

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M

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A

L

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D

E

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T

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G

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D

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A

A

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I

L

L

E

E

T

P

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I

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Q

G

G

E

E

G

G

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N

I

V

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A

P

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P

I

P

E

G

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Y

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T

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A

L

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K

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K

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A

L

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|
Q

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M

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R

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T

G

G

T

K

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M

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A

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E

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I

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|
K

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A

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G

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I

A

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E

E

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-

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-

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-

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P

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F

E

L

H

H

N

T

G

T

T
|
T

F

F

R

G

G

G

N

N

N

P

L

L

A

A

F

C

V

A

T

A

A

A

K

L

D

A

A

T

I

I

D

N

H

V

Y

L

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L

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E

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D

N

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L

F

-

S

P

N

A

E

Q

V

A

K

E

A

Q

K

K

G

G

E

D

Y

M

V

L

K

L

E

D

Q

G

L

F

I

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D

Q

I

L

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A

G

R

Q

E

Y

Y

G

P

E

D

G

L

N

V

F

Q

T

E

N

A

K

R

G

G

R

K

G

G

M

M

F

L

Q

M

G

A

I

I

N

E

C

F

V

V

N

D

G

N

E

E

I

I

A

G

A

Y

K

N

I

F

T

A

K

S

K

E

A

M

F

F

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K

Q

G

R

M

V

I

L

I

V

E

A

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G

S

T

G

L

A

N

D

N

D

A

Q

K

I

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V

I

K

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L

I

F

E

C

P

P

P

L

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T

I

L

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I

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Q

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A

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R

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S

A

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L

GT 150:151 (= GT 109:110) binding in other chain
Q274 (= Q238) binding in other chain
K300 (= K264) modified: N6-(pyridoxal phosphate)lysine
T332 (= T293) binding pyridoxal 5'-phosphate

4uoxA Crystal structure of ygjg in complex with pyridoxal-5'-phosphate and putrescine (see paper)
28% identity, 89% coverage: 30:411/430 of query aligns to 70:443/453 of 4uoxA

query
sites
4uoxA

M

L

W

V

D

D

E

T

E

Q

G

G

N

Q

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F

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I

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D

F

C

L

L

A

G

G

G

A

F

G

G

T

I

L

F

N

N

Y

V

G

G

H

H

N

R

N

N

D

P

H

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V

A

S

N

A

L

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M

Q

K

N

Y

Q

I

L

M

A

E

K

D

Q

G

-

I

-

T

-

H

-

G

-

L

-

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P

M

L

H

H

T

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Q

A

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K

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G

L

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F

P

L

L

N

R

T

A

F

M

N

L

E

A

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K

I

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L

K

A

-

A

-

L

-

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P

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G

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K

M

L

E

K

Y

Y

M

-

V

-

Q

S

F

F

T

F

G

C

P

N

T
x
S

G
|
G

T
|
T

N

E

A

S

V

V

E

E

A

A

M

L

K

K

L

L

A

A

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K

N

A

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G

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Q

P

Q

R

N

G

I

K

V

F

T

T

F

F

-

I

-

A

-

T

T

S

N

G

G

A

F
|
F

H
|
H

G
|
G

V

K

S

S

L

L

G

G

A

A

L

L

S

S

A

A

T

T

G

A

N

K

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S

H

T

H

F

R

R

D

K

A

P

A

F

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M

V

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S

L

L

L

N

P

G

G

I

F

H

R

R

H

M

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P

F

F

D

G

G

-

Y

-

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-

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-

G

-

I

-

D

-

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N

T

I

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E

Y

A

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M

D

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K

T

V

A

L

L

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D

E

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K

G

T

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G

N

D

S

D

P

V

A

A

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V

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I

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L

E
|
E

T

P

V

I

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Q

G

G

E
|
E

G

G

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V

N

I

V

L

A

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S

P

I

P

E

G

W

Y

L

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A

L

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K

V

L

C

C

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D

K

E

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F

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G

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A

L

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I

I

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|
D

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E

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x
V

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|
Q

A

T

G

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M

G

G

R

R

T

T

G

G

T

K

F

M

F

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A

F

C

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E

E

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S

E

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N

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P

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D

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I

I

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|
K

S

A

I

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S

G

G

G

Y

G

G

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L

M

P

P

F

I

A

G

V

A

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I

M

A

K

T

P

E

E

E

L

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D

F

Q

S

W

V

-

L

-

F

-

D

K

N

P

P

G

F

E

L

H

H

N

T

G
x
T

T
|
T

F

F

R

G

G

G

N

N

N

P

L

L

A

A

F

C

V

A

T

A

A

A

K

L

D

A

A

T

I

I

D

N

H

V

Y

L

W

L

S

E

D

Q

D

N

V

L

F

-

S

P

N

A

E

Q

V

A

K

E

A

Q

K

K

G

G

E

D

Y

M

V

L

K

L

E

D

Q

G

L

F

I

R

D

Q

I

L

V

A

G

R

Q

E

Y

Y

G

P

E

D

G

L

N

V

F

Q

T

E

N

A

K

R

G

G

R

K

G

G

M

M

F

L

Q

M

G

A

I

I

N

E

C

F

V

V

N

D

G

N

E

E

I

I

A

G

A

Y

K

N

I

F

T

A

K

S

K

E

A

M

F

F

S

R

K

Q

G

R

M

V

I

L

I

V

E

A

T

G

S

T

G

L

A

N

D

N

D

A

Q

K

I

T

V

I

K
x
R

L

I

F

E

C

P

P

P

L

L

T

T

I

L

T

T

Q

I

E

E

N

Q

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C

E

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K

L

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V

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K

I

A

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A

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R

E

K

S

A

I

L

active site: F174 (= F136), E232 (= E202), D265 (= D235), Q268 (= Q238), K294 (= K264), T326 (= T293), R420 (≠ K388)
binding pyridoxal-5'-phosphate: S143 (≠ T108), G144 (= G109), T145 (= T110), F174 (= F136), H175 (= H137), G176 (= G138), D265 (= D235), V267 (≠ I237), Q268 (= Q238), T325 (≠ G292), T326 (= T293)
binding 1,4-diaminobutane: E237 (= E207), K294 (= K264)

Sites not aligning to the query:

active site: 44

8r2pD Yzwideal x16 a scaffold for cryo-em of small proteins of interest crystallizing in space group 19 (p 21 21 21)
28% identity, 89% coverage: 30:411/430 of query aligns to 69:442/507 of 8r2pD

query
sites
8r2pD

M

L

W

V

D

D

E

T

E

Q

G

G

N

Q

R

E

Y

F

L

I

D

D

F

C

L

L

A

G

G

G

A

F

G

G

T

I

L

F

N

N

Y

V

G

G

H

H

N

R

N

N

D

P

H

V

L

V

K

V

A

S

N

A

L

V

M

Q

K

N

Y

Q

I

L

M

A

E

K

D

Q

G

P

I

L

T

-

H

H

G

S

L

Q

D

E

M

L

H

L

T

D

K

P

A

L

K

R

G

A

D

M

F

L

L

A

N

K

T

T

F

L

N

-

E

-

K

A

I

A

L

L

K

T

P

P

R

G

D

K

M

L

E

K

Y

Y

M

-

V

-

Q

S

F

F

T

F

G

C

P

N

T
x
S

G
|
G

T
|
T

N

E

A

S

V

V

E

E

A

A

M

L

K

K

L

L

A

A

R

K

N

A

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T

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G

S

Q

P

Q

R

N

G

I

K

V

F

T

T

F

F

-

I

-

A

-

T

T

S

N

G

G

A

F
|
F

H
|
H

G
|
G

V

K

S

S

L

L

G

G

A

A

L

L

S

S

A

A

T

T

G

A

N

K

S

S

H

T

H

F

R

R

D

K

A

P

A

F

G

M

V

P

S

L

L

L

N

P

G

G

I

F

H

R

R

H

M

V

P

P

F

F

D

G

G

-

Y

-

L

-

G

-

D

-

G

-

I

-

D

-

T

N

T

I

E

E

Y

A

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M

D

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A

L

L

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N

D

E

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G

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A

A

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L

I

L

L

E

E

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P

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I

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Q

G

G

E

E

G

G

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N

I

V

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A

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S

P

I

P

E

G

W

Y

L

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T

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A

L

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K

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C

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K

E

H

F

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G

L

A

L

L

L

M

I

I

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L

D
|
D

D

E

I
x
V

Q
|
Q

A

T

G

G

C

M

G

G

R

R

T

T

G

G

T

K

F

M

F

F

S

A

F

C

E

E

E

H

S

E

G

N

I

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Q

P

P

D

D

I

I

I

L

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M

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A

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|
K

S

A

I

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S

G

G

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Y

G

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M

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P

F

I

A

G

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A

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M

A

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T

P

E

E

E

L

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F

Q

S

W

V

-

L

-

F

-

D

K

N

P

P

G

F

E

L

H

H

N

T

G

T

T

T

F

F

R

G

G

G

N

N

N

P

L

L

A

A

F

C

V

A

T

A

A

A

K

L

D

A

A

T

I

I

D

N

H

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Y

L

W

L

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E

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Q

D

N

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L

F

-

S

P

N

A

E

Q

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A

K

E

A

Q

K

K

G

G

E

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Y

M

V

L

K

L

E

D

Q

G

L

F

I

R

D

Q

I

L

V

A

G

R

Q

E

Y

Y

G

P

E

D

G

L

N

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F

Q

T

E

N

A

K

R

G

G

R

K

G

G

M

M

F

L

Q

M

G

A

I

I

N

E

C

F

V

V

N

D

G

N

E

E

I

I

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A

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K

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K

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binding pyridoxal-5'-phosphate: S142 (≠ T108), G143 (= G109), T144 (= T110), F173 (= F136), H174 (= H137), G175 (= G138), D264 (= D235), V266 (≠ I237), Q267 (= Q238), K293 (= K264)

2ordA Crystal structure of acetylornithine aminotransferase (ec 2.6.1.11) (acoat) (tm1785) from thermotoga maritima at 1.40 a resolution
28% identity, 93% coverage: 11:412/430 of query aligns to 12:391/393 of 2ordA

query
sites
2ordA

V

M

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Y

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active site: F134 (= F136), E186 (= E202), D219 (= D235), Q222 (= Q238), K248 (= K264), T276 (= T293), R367 (≠ K388)
binding pyridoxal-5'-phosphate: G102 (= G109), T103 (= T110), F134 (= F136), H135 (= H137), E186 (= E202), D219 (= D235), V221 (≠ I237), Q222 (= Q238), K248 (= K264)

Query Sequence

>WP_046010019.1 NCBI__GCF_000967895.1:WP_046010019.1
MKIFKEIESEVQSYARSFPCVFNRAKDEFMWDEEGNRYLDFLAGAGTLNYGHNNDHLKAN
LMKYIMEDGITHGLDMHTKAKGDFLNTFNEKILKPRDMEYMVQFTGPTGTNAVEAAMKLA
RNVTGQQNIVTFTNGFHGVSLGALSATGNSHHRDAAGVSLNGIHRMPFDGYLGDGIDTTE
YLDKVLSDSSSGINSPAAVLLETVQGEGGVNVASIEWLQNLEKVCRKHGLLLIVDDIQAG
CGRTGTFFSFEESGIKPDIITMSKSISGYGLPFAVVLMKPELDQWKPGEHNGTFRGNNLA
FVTAKDAIDHYWSDDVFSNEVKAKGEYVKEQLIDIVGQYGEGNFTNKGRGMFQGINCVNG
EIAAKITKKAFSKGMIIETSGADDQIVKLFCPLTITQENLEKGIEILRESIKEVCSKEGD
IPEEKLYFQD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory