SitesBLAST
Comparing WP_046061698.1 NCBI__GCF_000968535.2:WP_046061698.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3gayA Structure of giardia fructose-1,6-biphosphate aldolase in complex with tagatose-1,6-biphosphate (see paper)
46% identity, 99% coverage: 3:336/337 of query aligns to 2:319/319 of 3gayA
- binding 1,6-di-O-phosphono-D-tagatose: N23 (= N24), S49 (= S50), D82 (= D83), H174 (= H187), G175 (= G188), K178 (= K191), H206 (= H221), G207 (= G222), S209 (= S224), N249 (= N264), D251 (= D266), S252 (≠ T267), R255 (= R270)
- binding zinc ion: H83 (= H84), H174 (= H187), H206 (= H221)
A8B2U2 Fructose-bisphosphate aldolase; Glfba; glFBPA; Fructose-1,6-bisphosphate aldolase; EC 4.1.2.13 from Giardia intestinalis (strain ATCC 50803 / WB clone C6) (Giardia lamblia) (see 4 papers)
46% identity, 100% coverage: 1:336/337 of query aligns to 1:323/323 of A8B2U2
- S50 (= S50) binding
- D83 (= D83) active site, Proton donor; mutation to A: Severe loss of catalytic activity.
- H84 (= H84) binding
- H178 (= H187) binding ; binding
- G179 (= G188) binding
- K182 (= K191) binding
- H210 (= H221) binding
- G211 (= G222) binding
- S213 (= S224) binding
- N253 (= N264) binding
- D255 (= D266) binding ; mutation to A: 9.4-fold reduction in substrate affinity and 50-fold reduction in catalytic affinity. Has some activity towards tagatose-1,6-bisphosphate.
- S256 (≠ T267) binding
- R259 (= R270) binding ; mutation to A: 1.8-fold reduction in substrate affinity and 2.8-fold reduction in catalytic efficiency. 6-fold reduction in substrate affinity and 24-fold reduction in catalytic efficiency; when associated with A-278.
- D278 (= D291) mutation to A: 159-fold reduction in substrate affinity and 2770-fold reduction in catalytic efficiency. 6-fold reduction in substrate affinity and 24-fold reduction in catalytic efficiency; when associated with A-259.
- R280 (= R293) binding
3ohiA Structure of giardia fructose-1,6-biphosphate aldolase in complex with 3-hydroxy-2-pyridone (see paper)
46% identity, 99% coverage: 3:336/337 of query aligns to 2:319/319 of 3ohiA
- binding ({3-hydroxy-2-oxo-4-[2-(phosphonooxy)ethyl]pyridin-1(2H)-yl}methyl)phosphonic acid: S49 (= S50), D82 (= D83), H83 (= H84), H174 (= H187), G175 (= G188), K178 (= K191), G207 (= G222), S209 (= S224), N249 (= N264), D251 (= D266), S252 (≠ T267), R255 (= R270)
- binding zinc ion: H83 (= H84), H174 (= H187), H206 (= H221)
3gb6A Structure of giardia fructose-1,6-biphosphate aldolase d83a mutant in complex with fructose-1,6-bisphosphate (see paper)
46% identity, 99% coverage: 3:336/337 of query aligns to 2:318/318 of 3gb6A
- binding 1,6-di-O-phosphono-D-fructose: N23 (= N24), S49 (= S50), H173 (= H187), G174 (= G188), K177 (= K191), H205 (= H221), G206 (= G222), S208 (= S224), N248 (= N264), D250 (= D266), S251 (≠ T267), R254 (= R270)
2isvB Structure of giardia fructose-1,6-biphosphate aldolase in complex with phosphoglycolohydroxamate (see paper)
45% identity, 99% coverage: 3:335/337 of query aligns to 2:307/307 of 2isvB
- binding phosphoglycolohydroxamic acid: D82 (= D83), H168 (= H187), G169 (= G188), K172 (= K191), H195 (= H221), G196 (= G222), S198 (= S224), N238 (= N264), D240 (= D266), S241 (≠ T267)
- binding zinc ion: H83 (= H84), H168 (= H187), H195 (= H221)
2isvA Structure of giardia fructose-1,6-biphosphate aldolase in complex with phosphoglycolohydroxamate (see paper)
44% identity, 99% coverage: 3:335/337 of query aligns to 2:298/298 of 2isvA
3n9sA Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with n-(4-hydroxybutyl)- glycolohydroxamic acid bis- phosphate, a competitive inhibitor (see paper)
39% identity, 93% coverage: 3:317/337 of query aligns to 2:304/307 of 3n9sA
- active site: C69 (≠ V70), E70 (= E71), G136 (= G144), H180 (= H187), A226 (≠ Y237), N253 (= N264)
- binding calcium ion: D104 (= D105), S106 (= S107), E134 (= E142)
- binding 4-{hydroxy[(phosphonooxy)acetyl]amino}butyl dihydrogen phosphate: N23 (= N24), S49 (= S50), D82 (= D83), H83 (= H84), H180 (= H187), G181 (= G188), K184 (= K191), H210 (= H221), G211 (= G222), S213 (= S224), N253 (= N264), D255 (= D266), T256 (= T267)
- binding zinc ion: H83 (= H84), H180 (= H187), H210 (= H221)
5ucpA Class ii fructose-1,6-bisphosphate aldolase e142a variant of helicobacter pylori with fbp and cleavage products (see paper)
39% identity, 93% coverage: 3:317/337 of query aligns to 2:289/292 of 5ucpA
- binding 1,6-di-O-phosphono-D-fructose: S49 (= S50), D82 (= D83), H83 (= H84), H165 (= H187), K169 (= K191), G196 (= G222), S198 (= S224), N238 (= N264), D240 (= D266), T241 (= T267), R244 (= R270)
- binding zinc ion: H83 (= H84), H83 (= H84), H83 (= H84), E134 (= E142), H165 (= H187), H165 (= H187), H165 (= H187), H195 (= H221), H195 (= H221)
3c52A Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with phosphoglycolohydroxamic acid, a competitive inhibitor (see paper)
39% identity, 93% coverage: 3:317/337 of query aligns to 2:293/296 of 3c52A
- active site: C69 (≠ V70), E70 (= E71), G136 (= G144), H169 (= H187), A215 (≠ Y237), N242 (= N264)
- binding calcium ion: D104 (= D105), S106 (= S107), E134 (= E142)
- binding phosphoglycolohydroxamic acid: D82 (= D83), H83 (= H84), H169 (= H187), K173 (= K191), H199 (= H221), G200 (= G222), S202 (= S224), N242 (= N264), D244 (= D266), T245 (= T267)
- binding zinc ion: H83 (= H84), H169 (= H187), H199 (= H221)
3n9rA Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with n-(4-hydroxybutyl)-phosphoglycolohydroxamic acid, a competitive inhibitor (see paper)
39% identity, 93% coverage: 3:317/337 of query aligns to 2:294/297 of 3n9rA
- active site: C69 (≠ V70), E70 (= E71), G136 (= G144), H170 (= H187), A216 (≠ Y237), N243 (= N264)
- binding 2-[hydroxy(4-hydroxybutyl)amino]-2-oxoethyl dihydrogen phosphate: H83 (= H84), H170 (= H187), G171 (= G188), K174 (= K191), H200 (= H221), G201 (= G222), S203 (= S224), N243 (= N264), D245 (= D266), T246 (= T267)
- binding zinc ion: H83 (= H84), H170 (= H187), H200 (= H221)
3c56A Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with n-(3-hydroxypropyl)-glycolohydroxamic acid bisphosphate, a competitive inhibitor (see paper)
39% identity, 93% coverage: 3:317/337 of query aligns to 2:294/297 of 3c56A
- active site: C69 (≠ V70), E70 (= E71), G136 (= G144), H170 (= H187), A216 (≠ Y237), N243 (= N264)
- binding 3-{hydroxy[(phosphonooxy)acetyl]amino}propyl dihydrogen phosphate: N23 (= N24), S49 (= S50), D82 (= D83), H170 (= H187), K174 (= K191), G201 (= G222), S203 (= S224), N243 (= N264), D245 (= D266), T246 (= T267), R249 (= R270)
- binding zinc ion: H83 (= H84), H170 (= H187), H200 (= H221)
5uckA Class ii fructose-1,6-bisphosphate aldolase of helicobacter pylori with cleavage products (see paper)
38% identity, 93% coverage: 3:317/337 of query aligns to 2:288/291 of 5uckA
- binding glyceraldehyde-3-phosphate: S49 (= S50), D82 (= D83), H83 (= H84), H164 (= H187), D239 (= D266), R243 (= R270)
- binding zinc ion: H83 (= H84), H83 (= H84), E134 (= E142), H164 (= H187), H194 (= H221), H194 (= H221)
5ud4A Class ii fructose-1,6-bisphosphate aldolase h180q variant of helicobacter pylori with tbp (see paper)
38% identity, 93% coverage: 3:317/337 of query aligns to 2:290/293 of 5ud4A
- binding 1,6-di-O-phosphono-D-tagatose: S49 (= S50), D82 (= D83), Q166 (≠ H187), G167 (= G188), K170 (= K191), G197 (= G222), S199 (= S224), N239 (= N264), D241 (= D266), T242 (= T267), R245 (= R270)
- binding zinc ion: H83 (= H84), H83 (= H84), E134 (= E142), H196 (= H221), H196 (= H221)
1rv8B Class ii fructose-1,6-bisphosphate aldolase from thermus aquaticus in complex with cobalt (see paper)
40% identity, 94% coverage: 3:320/337 of query aligns to 2:305/305 of 1rv8B
- active site: D80 (= D83), H81 (= H84), E140 (= E150), H178 (= H187), H208 (= H221), N251 (= N264)
- binding cobalt (ii) ion: H81 (= H84), E132 (= E142), H178 (= H187), H208 (= H221)
- binding sulfate ion: R116 (= R126), H123 (= H133), S211 (= S224), D253 (= D266), T254 (= T267)
5ud0A Class ii fructose-1,6-bisphosphate aldolase e149a variant of helicobacter pylori with cleavage products (see paper)
37% identity, 93% coverage: 3:317/337 of query aligns to 2:279/282 of 5ud0A
P13243 Probable fructose-bisphosphate aldolase; FBP aldolase; FBPA; Fructose-1,6-bisphosphate aldolase; EC 4.1.2.13 from Bacillus subtilis (strain 168) (see paper)
35% identity, 95% coverage: 1:320/337 of query aligns to 1:285/285 of P13243
- T212 (≠ S224) modified: Phosphothreonine
- T234 (= T267) modified: Phosphothreonine
P0AB74 D-tagatose-1,6-bisphosphate aldolase subunit KbaY; TBPA; TagBP aldolase; D-tagatose-bisphosphate aldolase class II; Ketose 1,6-bisphosphate aldolase class II; Tagatose-bisphosphate aldolase; EC 4.1.2.40 from Escherichia coli (strain K12) (see paper)
33% identity, 94% coverage: 1:318/337 of query aligns to 1:282/286 of P0AB74
- D82 (= D83) active site, Proton donor
- H83 (= H84) binding
- H180 (= H187) binding
- H208 (= H221) binding
8q59A Crystal structure of metal-dependent class ii sulfofructose phosphate aldolase from yersinia aldovae in complex with sulfofructose phosphate (yasqia-zn-sfp) (see paper)
35% identity, 95% coverage: 1:320/337 of query aligns to 1:276/280 of 8q59A
- binding (3~{S},4~{S})-2,3,4-tris(oxidanyl)-5-oxidanylidene-6-phosphonooxy-hexane-1-sulfonic acid: G50 (≠ S50), Q51 (≠ A51), K52 (≠ G52), D82 (= D83), H83 (= H84), H172 (= H187), G173 (= G188), H200 (= H221), G201 (= G222), S203 (= S224), N222 (= N264), D224 (= D266), T225 (= T267)
- binding zinc ion: H83 (= H84), H172 (= H187), H200 (= H221)
1gvfB Structure of tagatose-1,6-bisphosphate aldolase (see paper)
33% identity, 94% coverage: 2:317/337 of query aligns to 1:272/275 of 1gvfB
- active site: D81 (= D83), H82 (= H84), H171 (= H187), H199 (= H221), N221 (= N264)
- binding phosphoglycolohydroxamic acid: D81 (= D83), H82 (= H84), H171 (= H187), G172 (= G188), H199 (= H221), G200 (= G222), S202 (= S224), N221 (= N264), V222 (≠ I265), A223 (≠ D266), T224 (= T267)
- binding zinc ion: H82 (= H84), H171 (= H187), H199 (= H221)
4to8A Methicillin-resistant staphylococcus aureus class iib fructose 1,6- bisphosphate aldolase (see paper)
33% identity, 94% coverage: 3:320/337 of query aligns to 2:277/279 of 4to8A
Query Sequence
>WP_046061698.1 NCBI__GCF_000968535.2:WP_046061698.1
MALVSLRQLLDYAAEHGFGIPAFNISNMEQVQAIMQAADACDSPVIMQGSAGANRYAGEV
FLRHLIMATVEQYPHIPVVMHRDHAPSPDICAQAIQSGFSSVMMDGSLLEDMKTPASFDY
NVDVTRTVVKMAHACGVSVEGEIGCLGSLENKTGQATNRLLTDPEEAVEFVEQTQVDALA
VAIGTSHGAYKFSKPPTGEVLVISRLRELQQRLPNTHFVMHGSSSVPQDWLKVINEYGGD
IPQTYGVPVNEIVEGIKYGVRKVNIDTDLRMASTGAIRRYIADPTNASELDSRKIYQSAR
DAMQALCQSRYEAFGSAGHAGKIKVVPLRDMAKRYQG
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory