SitesBLAST
Comparing WP_046104561.1 NCBI__GCF_000969445.1:WP_046104561.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4hcxA Structure of icdh-1 from m.Tuberculosis complexed with NADPH & mn2+ (see paper)
70% identity, 99% coverage: 3:400/403 of query aligns to 2:401/402 of 4hcxA
- binding manganese (ii) ion: D274 (= D273), D278 (= D277)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A73 (= A74), T74 (= T75), T76 (= T77), E305 (= E304), H308 (= H307), G309 (= G308), T310 (= T309), V311 (= V310), R313 (= R312), H314 (= H313), N327 (= N326)
P33198 Isocitrate dehydrogenase [NADP], mitochondrial; IDH; ICD-M; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase; EC 1.1.1.42 from Sus scrofa (Pig) (see 2 papers)
67% identity, 100% coverage: 3:403/403 of query aligns to 12:419/421 of P33198
- T86 (= T77) binding D-threo-isocitrate
- 103:109 (vs. 94:100, 100% identical) binding D-threo-isocitrate
- R118 (= R109) binding D-threo-isocitrate
- R141 (= R132) binding D-threo-isocitrate
- Y148 (= Y139) Critical for catalysis; mutation to F: Large decrease in Vmax and insignificant change in KM for isocitrate and NADP.
- K220 (= K210) Critical for catalysis; mutation K->Q,Y: Large decrease in Vmax and insignificant change in KM for isocitrate and NADP.
- D260 (= D250) binding Mn(2+)
- D283 (= D273) binding Mn(2+)
1t09A Crystal structure of human cytosolic NADP(+)-dependent isocitrate dehydrogenase in complex NADP (see paper)
66% identity, 100% coverage: 1:403/403 of query aligns to 1:410/414 of 1t09A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: A74 (= A74), T75 (= T75), I76 (= I76), T77 (= T77), R82 (= R82), N96 (= N96), D279 (= D277), S280 (≠ I278), H309 (= H307), G310 (= G308), V312 (= V310), R314 (= R312), T327 (= T325), N328 (= N326)
O75874 Isocitrate dehydrogenase [NADP] cytoplasmic; IDH; IDH1; Cytosolic NADP-isocitrate dehydrogenase; IDPc; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase; EC 1.1.1.42 from Homo sapiens (Human) (see 6 papers)
66% identity, 100% coverage: 1:403/403 of query aligns to 1:410/414 of O75874
- TIT 75:77 (= TIT 75:77) binding NADP(+)
- T77 (= T77) binding in other chain
- R82 (= R82) binding NADP(+)
- 94:100 (vs. 94:100, 100% identical) binding in other chain
- R109 (= R109) binding in other chain
- R132 (= R132) binding in other chain; to C: in colorectal cancer and glioma samples; glioblastoma multiforme; somatic mutation; found in patients with cartilaginous tumors; abolishes magnesium binding and alters enzyme activity so that isocitrate is no longer converted to alpha-ketoglutarate but instead alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate; induces histone methylation; enhances expression of chondrocyte-related genes; disturbs the formation of cartilaginous matrix; inhibits osteogenic differentiation; dbSNP:rs121913499; to G: in a glioma sample; glioblastoma multiforme; somatic mutation; found in patients with cartilaginous tumors; dbSNP:rs121913499; to H: in a glioma sample; glioblastoma multiforme; somatic mutation; found in patients with cartilaginous tumors; abolishes magnesium binding and alters enzyme activity so that isocitrate is no longer converted to alpha-ketoglutarate but instead alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate; dbSNP:rs121913500; to L: in a glioma sample; glioblastoma multiforme; somatic mutation; abolishes magnesium binding and alters enzyme activity so that isocitrate is no longer converted to alpha-ketoglutarate but instead alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate; dbSNP:rs121913500; to S: in a glioma sample; glioblastoma multiforme; somatic mutation; abolishes magnesium binding and alters enzyme activity so that isocitrate is no longer converted to alpha-ketoglutarate but instead alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate; dbSNP:rs121913499
- K212 (= K210) binding substrate
- K260 (= K258) binding NADP(+)
- GTVTRH 310:315 (= GTVTRH 308:313) binding NADP(+)
- N328 (= N326) binding NADP(+)
P48735 Isocitrate dehydrogenase [NADP], mitochondrial; IDH; ICD-M; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase; EC 1.1.1.42 from Homo sapiens (Human) (see 4 papers)
66% identity, 100% coverage: 3:403/403 of query aligns to 43:450/452 of P48735
- P158 (= P118) to L: in GLM; somatic mutation; dbSNP:rs2151549649
- P162 (= P122) to S: in GLM; somatic mutation; dbSNP:rs1382680247
- R172 (= R132) to G: in GLM; somatic mutation; reduces enzymatic activity; dbSNP:rs1057519906; to K: in GLM; somatic mutation; reduces enzymatic activity; dbSNP:rs121913503; to M: in GLM; somatic mutation; reduces enzymatic activity; dbSNP:rs121913503; to S: found in patients with cartilagenous tumors; dbSNP:rs1057519736; to T: found in patients with cartilagenous tumors; dbSNP:rs121913503; to W: found in patients with cartilagenous tumors; dbSNP:rs1057519906
- K413 (= K372) modified: N6-acetyllysine; mutation to A: 44-fold loss in activity.; mutation to Q: 20-fold decrease in Vmax.; mutation to R: No appreciable difference in Km for isocitrate and NADP.
5h3eB Crystal structure of mouse isocitrate dehydrogenases 2 k256q mutant complexed with isocitrate (see paper)
66% identity, 100% coverage: 3:403/403 of query aligns to 2:409/410 of 5h3eB
6ajcA Crystal structure of trypanosoma cruzi cytosolic isocitrate dehydrogenase in complex with NADP+, isocitrate and ca2+
66% identity, 99% coverage: 3:401/403 of query aligns to 3:407/413 of 6ajcA
- binding calcium ion: D274 (= D273), D278 (= D277)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: T75 (= T75), I76 (= I76), T77 (= T77), N96 (= N96), D252 (= D251), K259 (= K258), L287 (= L286), A307 (= A306), H308 (= H307), G309 (= G308), T310 (= T309), V311 (= V310), R313 (= R312), H314 (= H313), N327 (= N326)
8vhaA Crystal structure of human idh1 r132q in complex with NADPH and alpha- ketoglutarate (see paper)
66% identity, 100% coverage: 1:403/403 of query aligns to 2:411/414 of 8vhaA
- binding calcium ion: D276 (= D273), D280 (= D277)
- binding (3~{S})-3-[(4~{S})-3-aminocarbonyl-1-[(2~{R},3~{R},4~{S},5~{R})-5-[[[[(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]-4~{H}-pyridin-4-yl]-2-oxidanylidene-pentanedioic acid: A75 (= A74), T76 (= T75), T78 (= T77), S95 (= S94), N97 (= N96), H310 (= H307), G311 (= G308), T312 (= T309), V313 (= V310), R315 (= R312), H316 (= H313), N329 (= N326)
8vheB Isocitrate dehydrogenase [NADP] cytoplasmic (see paper)
66% identity, 100% coverage: 1:403/403 of query aligns to 10:419/421 of 8vheB
- binding 3,3',3''-({(4R)-1-[(2R,3R,4S,5R)-5-({[(S)-{[(S)-{[(2R,3R,4R,5R)-5-(6-amino-9H-purin-9-yl)-3-hydroxy-4-(phosphonooxy)oxolan-2-yl]methoxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]oxy}methyl)-3,4-dihydroxyoxolan-2-yl]-3-carbamoyl-1,4-dihydropyridin-4-yl}-lambda~5~-phosphanetriyl)tripropanoic acid: T84 (= T75), I85 (= I76), T86 (= T77), S103 (= S94), N105 (= N96), R109 (= R100), R118 (= R109), D288 (= D277), A317 (= A306), H318 (= H307), G319 (= G308), T320 (= T309), V321 (= V310), T322 (= T311), R323 (= R312), H324 (= H313), T336 (= T325), N337 (= N326)
- binding calcium ion: D284 (= D273), D288 (= D277)
5svoA Structure of idh2 mutant r140q (see paper)
66% identity, 100% coverage: 3:403/403 of query aligns to 2:409/410 of 5svoA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: T74 (= T75), T76 (= T77), H307 (= H307), G308 (= G308), T309 (= T309), V310 (= V310), T311 (= T311), R312 (= R312), H313 (= H313), T325 (= T325), N326 (= N326)
6bl2A Novel modes of inhibition of wild-type idh1: direct covalent modification of his315 with cmpd15 (see paper)
67% identity, 98% coverage: 10:403/403 of query aligns to 8:408/416 of 6bl2A