SitesBLAST
Comparing WP_046156002.1 NCBI__GCF_000971335.1:WP_046156002.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 8 hits to proteins with known functional sites (download)
P0AGC0 Hexose-6-phosphate:phosphate antiporter from Escherichia coli (strain K12) (see paper)
32% identity, 92% coverage: 24:433/447 of query aligns to 20:442/463 of P0AGC0
- C108 (≠ L108) mutation to S: No change in activity.
- C143 (≠ A140) mutation to S: 30% of wild-type sugar phosphate transport activity.
- C265 (≠ A259) mutation to S: No change in activity.
- C331 (≠ V326) mutation to S: No change in activity.
- C436 (≠ A427) mutation to S: No change in activity.
- C438 (≠ V429) mutation to S: No change in activity.
P0AA76 D-galactonate transporter; D-galactonate/H(+) symporter from Escherichia coli (strain K12) (see paper)
24% identity, 87% coverage: 25:413/447 of query aligns to 12:401/430 of P0AA76
- Y29 (= Y42) binding D-galactonate
- D31 (≠ V44) mutation to N: Loss of galactonate transport activity.
- R32 (= R45) binding D-galactonate
- Y64 (= Y76) binding D-galactonate
- E118 (≠ Q134) mutation to Q: Loss of galactonate transport activity.
- W358 (≠ G369) binding D-galactonate
6e9nA E. Coli d-galactonate:proton symporter in the inward open form (see paper)
24% identity, 87% coverage: 25:413/447 of query aligns to 1:382/409 of 6e9nA
6e9oA E. Coli d-galactonate:proton symporter mutant e133q in the outward substrate-bound form (see paper)
24% identity, 87% coverage: 25:413/447 of query aligns to 4:366/393 of 6e9oA
8u3hA Structure of fmoc-leu-oh bound sialin (see paper)
25% identity, 59% coverage: 27:291/447 of query aligns to 4:264/425 of 8u3hA
Sites not aligning to the query:
8u3gA Structure of naag-bound sialin (see paper)
25% identity, 59% coverage: 27:291/447 of query aligns to 4:266/427 of 8u3gA
Sites not aligning to the query:
Q9NRA2 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Homo sapiens (Human) (see 8 papers)
23% identity, 64% coverage: 8:291/447 of query aligns to 20:327/495 of Q9NRA2
- LL 22:23 (≠ HL 10:11) Dileucine internalization motif; mutation to AA: Targeted to plasma membrane.; mutation to GG: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
- R39 (= R27) to C: in SD; frequent variant in Finland; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transport activity, but retains appreciable H(+)-coupled sialic acid and nitrate transporter activity; dbSNP:rs80338794
- K136 (≠ R93) to E: in SD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transporter activity, but retains appreciable H(+)-coupled sialic acid transporter activity; dbSNP:rs80338795
- H183 (≠ G142) to R: in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity; dbSNP:rs119491109
- LL 198:199 (≠ VV 157:158) mutation to AA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
- IL 266:267 (≠ DY 230:231) mutation to LA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
- SSLRN 268:272 (≠ SDTHE 232:236) natural variant: Missing (in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity)
Sites not aligning to the query:
- 328 G → E: in ISSD; some patients may manifest a milder phenotype consistent with Salla disease; markedly decreases H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs386833996
- 334 P → R: in ISSD; does not affect intracellular localization, targeted to lysosomes; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs119491110
- 371 G → V: in ISSD; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs777862172
Q5EXK5 3-hydroxybenzoate transporter MhbT from Klebsiella oxytoca (see paper)
24% identity, 79% coverage: 84:434/447 of query aligns to 78:431/452 of Q5EXK5
- D82 (= D88) mutation to A: Loss of activity.
- V311 (≠ W311) mutation to W: Loss of activity.
- D314 (= D314) mutation to A: Loss of activity.
Query Sequence
>WP_046156002.1 NCBI__GCF_000971335.1:WP_046156002.1
MISLFKPAPHLPPLPDAQQHGLYRRLRWQIFLGIFLGYAGYYLVRKNFSLAMPYLVEQGF
SRGDLGFAMSGVAIAYGLSKFLMGAVSDRSNPRVFLSAGLVLSAAVFLLMGFAPWATSSV
GVMFVLLFLNGWFQGMGWPACGRTMVHWWSQKERGGVVSIWNCAHNVGGGLIGPLFILGM
GWFNDWRAAFYVPAAAAIGVAVFAWLTMRDTPQSCGLPPVEAYRNDYPEDYSDTHEQELT
AREIFRKYILPNKLLWYIALANVFIYLLRYGVLDWAPTYLKEVKHFSVDKSSWAYFLYEW
AGIPGTLLCGWMSDKLFRGNRGATGVFFMALVTLATVVYWLNPAGNPTVDMLALIAIGFL
IYGPVMLVGLHALELAPKKAAGTAAGFTGLFGYLGGSVAANAAVGYTVDHFGWDGGFALL
IGSCVAAIVLLALTTWHGHQQGKLASA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory