SitesBLAST
Comparing WP_046157099.1 NCBI__GCF_000971335.1:WP_046157099.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 9 hits to proteins with known functional sites (download)
7cyxA Crystal strcuture of glycine oxidase from bacillus cereus atcc 14579 (see paper)
22% identity, 83% coverage: 34:389/429 of query aligns to 3:346/363 of 7cyxA
- binding flavin-adenine dinucleotide: I7 (≠ V38), G8 (= G39), G10 (= G41), V11 (≠ L42), I12 (≠ A43), V30 (≠ L61), E31 (= E62), K32 (≠ G63), E38 (≠ G69), A39 (= A70), S40 (= S71), A43 (≠ N74), G45 (= G76), L46 (≠ Q77), V171 (≠ A213), G200 (≠ C240), G201 (≠ N241), W203 (≠ Y243), G298 (= G343), R300 (≠ C345), P301 (≠ D346), Y326 (≠ S369), R327 (≠ G370), N328 (≠ H371), G329 (= G372), I330 (≠ V373)
Q8GAI3 4-methylaminobutanoate oxidase (formaldehyde-forming); MABO; Demethylating gamma-N-methylaminobutyrate oxidase; Gamma-N-methylaminobutyrate oxidase 1; EC 1.5.3.19 from Paenarthrobacter nicotinovorans (Arthrobacter nicotinovorans) (see paper)
28% identity, 58% coverage: 1:248/429 of query aligns to 1:232/824 of Q8GAI3
- W66 (≠ G72) mutation W->F,S: Contains a non-covalently bound FAD. Loss of enzyme activity.
- H67 (≠ R73) mutation to A: Contains a non-covalently bound FAD. Exhibits about 10% of the wild-type enzyme activity.
5i39A High resolution structure of l-amino acid deaminase from proteus vulgaris with the deletion of the specific insertion sequence (see paper)
23% identity, 85% coverage: 33:395/429 of query aligns to 25:374/383 of 5i39A
- active site: F66 (≠ N74), Q69 (= Q77), A70 (≠ V78), Q248 (≠ R272), P267 (≠ D291)
- binding flavin-adenine dinucleotide: V30 (= V38), G31 (= G39), G33 (= G41), I34 (≠ L42), L35 (≠ A43), V53 (≠ L61), E54 (= E62), K55 (≠ G63), Q62 (≠ A70), S63 (= S71), F66 (≠ N74), Y67 (≠ G75), Q69 (= Q77), A196 (≠ P212), A197 (= A213), G226 (≠ C240), G227 (≠ N241), W229 (≠ Y243), Q248 (≠ R272), Q250 (≠ R274), G321 (= G343), M323 (≠ C345), T348 (≠ S369), G349 (= G370), W350 (≠ H371), G351 (= G372), M352 (≠ V373), T353 (≠ N374)
5hxwA L-amino acid deaminase from proteus vulgaris (see paper)
26% identity, 48% coverage: 33:239/429 of query aligns to 17:217/433 of 5hxwA
- active site: F58 (≠ N74), Q61 (= Q77), A62 (≠ V78)
- binding flavin-adenine dinucleotide: V22 (= V38), G23 (= G39), G25 (= G41), I26 (≠ L42), L27 (≠ A43), E46 (= E62), K47 (≠ G63), E53 (≠ G69), Q54 (≠ A70), S55 (= S71), R57 (= R73), F58 (≠ N74), Y59 (≠ G75), G60 (= G76), Q61 (= Q77), A188 (≠ P212), A189 (= A213)
Sites not aligning to the query:
- active site: 240, 284, 288
- binding cetyl-trimethyl-ammonium: 291, 294, 310, 311, 317, 318, 320, 373, 379, 399, 400
- binding flavin-adenine dinucleotide: 218, 219, 221, 240, 242, 331, 371, 373, 398, 399, 400, 401, 402, 403
1y56B Crystal structure of l-proline dehydrogenase from p.Horikoshii (see paper)
21% identity, 81% coverage: 48:396/429 of query aligns to 20:360/374 of 1y56B
- active site: F44 (≠ G72), G47 (= G75), T48 (≠ D87), H224 (≠ T260), P239 (≠ A275), G305 (= G343), M338 (≠ N374)
- binding flavin-adenine dinucleotide: I33 (≠ L61), E34 (= E62), K35 (≠ G63), S42 (≠ A70), T43 (≠ S71), R45 (= R73), C46 (≠ N74), G47 (= G75), G49 (= G88), E170 (≠ P212), V171 (≠ A213), T200 (≠ C240), N201 (= N241), W203 (≠ Y243), G305 (= G343), Y306 (≠ F344), Y307 (≠ C345), G334 (= G370), H335 (= H371), G336 (= G372), F337 (≠ V373), M338 (≠ N374)
- binding flavin mononucleotide: F44 (≠ G72), R45 (= R73), I260 (≠ D298), R301 (≠ Y339), W303 (= W341)
Sites not aligning to the query:
Q63342 Dimethylglycine dehydrogenase, mitochondrial; ME2GLYDH; EC 1.5.8.4 from Rattus norvegicus (Rat) (see 2 papers)
24% identity, 59% coverage: 23:273/429 of query aligns to 34:276/857 of Q63342
- CV 52:53 (≠ LA 42:43) binding FAD
- EK 73:74 (≠ EG 62:63) binding FAD
- 80:88 (vs. 69:77, 22% identical) binding FAD
- H84 (≠ R73) modified: Tele-8alpha-FAD histidine
- V212 (vs. gap) binding FAD
- W244 (≠ Y243) binding FAD
Sites not aligning to the query:
- 390:395 binding FAD
- 573:575 binding (6S)-5,6,7,8-tetrahydrofolate
- 669 binding (6S)-5,6,7,8-tetrahydrofolate
- 676:678 binding (6S)-5,6,7,8-tetrahydrofolate
- 737 binding (6S)-5,6,7,8-tetrahydrofolate
5fjnA Structure of l-amino acid deaminase from proteus myxofaciens in complex with anthranilate (see paper)
28% identity, 49% coverage: 31:239/429 of query aligns to 27:229/447 of 5fjnA
- active site: S67 (= S71), Y71 (≠ G75), S72 (≠ G76)
- binding flavin-adenine dinucleotide: I34 (≠ V38), G35 (= G39), G37 (= G41), I38 (≠ L42), Q39 (≠ A43), L57 (= L61), E58 (= E62), K59 (≠ G63), E65 (≠ G69), Q66 (≠ A70), S67 (= S71), A70 (≠ N74), Y71 (≠ G75), S72 (≠ G76), Q73 (= Q77), V201 (≠ A213)
Sites not aligning to the query:
- active site: 252
- binding 2-aminobenzoic acid: 252, 289, 411, 412
- binding flavin-adenine dinucleotide: 230, 231, 233, 252, 254, 343, 385, 410, 411, 412, 413, 414, 415
5fjmA Structure of l-amino acid deaminase from proteus myxofaciens (see paper)
28% identity, 49% coverage: 31:239/429 of query aligns to 27:229/447 of 5fjmA
- active site: S67 (= S71), Y71 (≠ G75), S72 (≠ G76)
- binding flavin-adenine dinucleotide: I34 (≠ V38), G35 (= G39), G37 (= G41), I38 (≠ L42), Q39 (≠ A43), L57 (= L61), E58 (= E62), K59 (≠ G63), E65 (≠ G69), Q66 (≠ A70), S67 (= S71), A70 (≠ N74), Y71 (≠ G75), S72 (≠ G76), Q73 (= Q77), V201 (≠ A213)
Sites not aligning to the query:
- active site: 252
- binding flavin-adenine dinucleotide: 230, 231, 233, 252, 254, 343, 385, 410, 411, 412, 413, 414, 415
4pabB Crystal structure of the precursor form of rat dmgdh complexed with tetrahydrofolate (see paper)
23% identity, 56% coverage: 33:273/429 of query aligns to 6:239/824 of 4pabB
- active site: T53 (≠ I79), E102 (= E141), H226 (≠ T260)
- binding flavin-adenine dinucleotide: I11 (≠ V38), G12 (= G39), G14 (= G41), C15 (≠ L42), V16 (≠ A43), L35 (= L61), E36 (= E62), K37 (≠ G63), G43 (= G69), S44 (≠ A70), T45 (≠ S71), H47 (≠ R73), A48 (≠ N74), A49 (≠ G75), G50 (= G76), L51 (≠ Q77), V175 (vs. gap), A204 (≠ C240), G205 (≠ N241), W207 (≠ Y243), H226 (≠ T260), Y228 (≠ V262)
Sites not aligning to the query:
- active site: 255, 536
- binding flavin-adenine dinucleotide: 326, 328, 353, 355, 356, 357, 358
- binding (6s)-5,6,7,8-tetrahydrofolate: 523, 536, 538, 550, 612, 613, 632, 639, 680, 700
Query Sequence
>WP_046157099.1 NCBI__GCF_000971335.1:WP_046157099.1
MLRFAHQPHPPSYYAATAHAWDPQPPLRGEASCDVCVVGGGLAGLSAALNLREKGFSVIL
LEGSHVGFGASGRNGGQVIAGYAGDMDGIRAQVGEEAAKALWDMSVEAVEIIDERVRRHG
IQCDWQRGYVSAAVKPRHMRELEDWQREAEERYDYGGMQLWDRAALRGKLASERYQGGLF
DPRSGHLHPLNYTLGLARAALAAGVDIHEQTPALRIEQGDAPKVFTEHGVVRCRHLVLAC
NSYIGALAPRLERRIMPAGTYVIATEPLGEERARALIADNMAVCDTNFVLDYYRLSADHR
LLFGGKVSYSGHEPRDLAGSMRADMLRVFPQLADVGIDYAWGGFCDITVNRAPDFGRLSG
NVYYLQGFSGHGVNITGLAGKVVAEAIAGTSSRLDLFAKIRHRDFPGGKLLRTPALVLGM
AYYRMRDYL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory