SitesBLAST
Comparing WP_046158297.1 NCBI__GCF_000971335.1:WP_046158297.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P02911 Lysine/arginine/ornithine-binding periplasmic protein; LAO-binding protein from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
44% identity, 100% coverage: 1:253/254 of query aligns to 1:259/260 of P02911
- D33 (= D29) binding L-arginine; binding L-lysine; binding L-ornithine; mutation to A: Decreases arginine and histidine binding affinity.
- Y36 (= Y32) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
- D52 (= D48) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
- C60 (= C56) modified: Disulfide link with 67
- C67 (= C63) modified: Disulfide link with 60
- F74 (≠ W70) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
- S91 (= S87) binding L-arginine; binding L-ornithine; mutation to A: Has no effect on the arginine and histidine binding affinity.
- S92 (= S88) binding L-arginine; binding L-lysine; binding L-ornithine; mutation to A: Decreases arginine and histidine binding affinity.
- S94 (≠ Q90) binding L-arginine; binding L-lysine; binding L-ornithine; mutation to A: Decreases arginine and histidine binding affinity.
- R99 (= R95) binding L-arginine; binding L-lysine; binding L-ornithine; mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
- L139 (= L133) mutation to K: Changes ligand specificity. Can bind glutamine. Still able to bind basic amino acids, however, with 1000-fold less affinity for arginine.; mutation to Q: Does not affect ligand preference.
- T143 (= T137) binding L-arginine; binding L-lysine; binding L-ornithine
- D183 (= D177) binding L-arginine; binding L-ornithine; mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
1lstA Three-dimensional structures of the periplasmic lysine-, arginine-, ornithine-binding protein with and without a ligand (see paper)
45% identity, 93% coverage: 19:253/254 of query aligns to 1:237/238 of 1lstA
1lahE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
45% identity, 93% coverage: 19:253/254 of query aligns to 1:237/238 of 1lahE
1lagE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
45% identity, 93% coverage: 19:253/254 of query aligns to 1:237/238 of 1lagE
- binding histidine: Y14 (= Y32), F52 (≠ W70), S69 (= S87), S70 (= S88), L71 (≠ M89), S72 (≠ Q90), R77 (= R95), L117 (= L133), S120 (= S136), Q122 (= Q138), D161 (= D177)
1lafE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
45% identity, 93% coverage: 19:253/254 of query aligns to 1:237/238 of 1lafE
- binding arginine: D11 (= D29), Y14 (= Y32), F52 (≠ W70), S69 (= S87), S70 (= S88), S72 (≠ Q90), R77 (= R95), L117 (= L133), S120 (= S136), T121 (= T137), Q122 (= Q138), D161 (= D177)
5owfA Structure of a lao-binding protein mutant with glutamine (see paper)
45% identity, 91% coverage: 22:253/254 of query aligns to 1:234/235 of 5owfA
P02910 Histidine-binding periplasmic protein; HBP from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
43% identity, 92% coverage: 22:254/254 of query aligns to 26:260/260 of P02910
- E40 (≠ S36) mutation to K: Decrease in ATPase-inducing activity and histidine transport; when associated with K-47.
- K42 (≠ Q38) mutation to E: Increases ATPase-inducing activity and histidine transport; when associated with K-47.
- E47 (≠ K43) mutation to K: Decrease in ATPase-inducing activity and histidine transport; when associated with K-40. Increases ATPase-inducing activity and histidine transport; when associated with E-42.
- D171 (= D165) mutation D->A,N: Strong decrease in ATPase-inducing activity and histidine transport.
- R176 (= R170) mutation R->D,S: Strong decrease in ATPase-inducing activity and histidine transport.
- D178 (= D172) mutation to A: Slight decrease in ATPase-inducing activity and histidine transport.
Sites not aligning to the query:
1hslA Refined 1.89 angstroms structure of the histidine-binding protein complexed with histidine and its relationship with many other active transport(slash)chemosensory receptors (see paper)
43% identity, 92% coverage: 22:254/254 of query aligns to 4:238/238 of 1hslA
P0AEU0 Histidine-binding periplasmic protein; HBP from Escherichia coli (strain K12) (see 3 papers)
43% identity, 92% coverage: 22:254/254 of query aligns to 26:260/260 of P0AEU0
- C60 (= C56) modified: Disulfide link with 67
- C67 (= C63) modified: Disulfide link with 60
- S91 (= S87) binding L-histidine
- S92 (= S88) binding L-histidine
- S94 (≠ Q90) binding L-histidine
- R99 (= R95) binding L-histidine
- T143 (= T137) binding L-histidine
- D183 (= D177) binding L-histidine
Sites not aligning to the query:
4zv1A An ancestral arginine-binding protein bound to arginine (see paper)
41% identity, 89% coverage: 23:247/254 of query aligns to 5:225/226 of 4zv1A
- binding arginine: E11 (≠ D29), F14 (≠ Y32), F52 (≠ W70), A69 (≠ S87), G70 (≠ S88), M71 (= M89), T72 (≠ Q90), R77 (= R95), Q117 (≠ L133), S120 (= S136), T121 (= T137), D161 (= D177)
4zv2A An ancestral arginine-binding protein bound to glutamine (see paper)
41% identity, 89% coverage: 23:247/254 of query aligns to 5:223/225 of 4zv2A
2y7iA Structural basis for high arginine specificity in salmonella typhimurium periplasmic binding protein stm4351. (see paper)
37% identity, 91% coverage: 18:248/254 of query aligns to 2:228/228 of 2y7iA
- binding arginine: Y16 (= Y32), E20 (≠ S36), F54 (≠ W70), A71 (≠ S87), G72 (≠ S88), D74 (≠ Q90), R79 (= R95), E118 (≠ L133), T121 (≠ S136), T122 (= T137), D160 (= D177)
- binding zinc ion: H9 (≠ R25), D23 (≠ G39), D25 (= D41), D55 (= D71), D67 (= D83), D140 (≠ A157)
3tqlA Structure of the amino acid abc transporter, periplasmic amino acid- binding protein from coxiella burnetii. (see paper)
40% identity, 89% coverage: 21:247/254 of query aligns to 1:225/225 of 3tqlA
5t0wA Crystal structure of the ancestral amino acid-binding protein anccdt- 1, a precursor of cyclohexadienyl dehydratase
36% identity, 89% coverage: 23:247/254 of query aligns to 11:228/229 of 5t0wA
- binding arginine: D17 (= D29), Y20 (= Y32), W58 (= W70), S75 (= S87), G76 (≠ S88), M77 (= M89), T78 (≠ Q90), R83 (= R95), Q126 (≠ L133), T129 (≠ S136), T130 (= T137), D168 (= D177)
8hqrA Crystal structure of the arginine-/lysine-binding protein sar11_1210 from 'candidatus pelagibacter ubique' htcc1062 bound to arginine (see paper)
31% identity, 91% coverage: 19:250/254 of query aligns to 5:264/267 of 8hqrA
- binding arginine: E15 (≠ D29), Y18 (= Y32), W56 (= W70), A73 (≠ S87), G74 (≠ S88), S76 (≠ Q90), R81 (= R95), E94 (≠ I108), Q143 (≠ L133), T146 (≠ S136), I147 (≠ T137)
5orgA Structure of the periplasmic binding protein (pbp) occj from a. Tumefaciens b6 in complex with octopine. (see paper)
33% identity, 91% coverage: 20:251/254 of query aligns to 2:254/257 of 5orgA
- binding octopine: E11 (≠ D29), Y14 (= Y32), W52 (= W70), A69 (≠ S87), A70 (≠ S88), M71 (= M89), S72 (≠ Q90), R77 (= R95), N92 (vs. gap), Q140 (≠ L133), T143 (≠ S136), T144 (= T137), N183 (≠ D177)
3vvfA Crystal structure of ttc0807 complexed with arginine (see paper)
30% identity, 90% coverage: 24:251/254 of query aligns to 19:238/241 of 3vvfA
- binding arginine: E24 (≠ D29), F27 (≠ Y32), F65 (≠ W70), A82 (≠ S87), S83 (= S88), H84 (≠ M89), G85 (≠ Q90), R90 (= R95), Q128 (≠ L133), T131 (≠ S136), T132 (= T137), Y133 (≠ Q138), E196 (≠ P209)
3vveA Crystal structure of ttc0807 complexed with lysine (see paper)
30% identity, 90% coverage: 24:251/254 of query aligns to 19:238/241 of 3vveA
- binding lysine: E24 (≠ D29), F27 (≠ Y32), F65 (≠ W70), S83 (= S88), H84 (≠ M89), R90 (= R95), Q128 (≠ L133), T131 (≠ S136), T132 (= T137), Y133 (≠ Q138), E196 (≠ P209)
3vvdA Crystal structure of ttc0807 complexed with ornithine (see paper)
30% identity, 90% coverage: 24:251/254 of query aligns to 19:238/241 of 3vvdA