SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_046158324.1 NCBI__GCF_000971335.1:WP_046158324.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 14 hits to proteins with known functional sites (download)

Q47945 Alcohol dehydrogenase (quinone), cytochrome c subunit; ADH; Alcohol dehydrogenase (quinone), subunit II; Cytochrome c-553; Cytochrome c553; Ethanol:Q2 reductase; G3-ADH subunit II; Quinohemoprotein-cytochrome c complex; Ubiquinol oxidase; EC 1.1.5.5 from Gluconobacter oxydans (strain 621H) (Gluconobacter suboxydans) (see paper)
46% identity, 97% coverage: 2:400/410 of query aligns to 19:434/478 of Q47945

query
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Q47945

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Q37 (≠ A20) modified: Pyrrolidone carboxylic acid

Sites not aligning to the query:

1:36 signal peptide

8gy2B Cryo-em structure of membrane-bound alcohol dehydrogenase from gluconobacter oxydans
47% identity, 91% coverage: 29:400/410 of query aligns to 6:396/433 of 8gy2B

query
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8gy2B

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binding heme c: C18 (= C41), C21 (= C44), H22 (= H45), T46 (= T69), I48 (= I71), Y59 (= Y82), L68 (= L91), R73 (≠ A96), V79 (≠ L102), Y80 (= Y103), M83 (= M106), F88 (= F111), R126 (= R149), H165 (= H187), C166 (= C188), C169 (= C191), H170 (= H192), I201 (≠ Y221), A202 (= A222), P203 (≠ G223), L205 (= L225), W216 (= W236), F224 (≠ Y244), A234 (= A254), V235 (≠ A255), F236 (= F256), F236 (= F256), M239 (= M259), N301 (= N306), C302 (= C307), C305 (= C310), H306 (= H311), M316 (≠ V321), F317 (≠ V322), P318 (= P323), L320 (= L325), P324 (≠ S329), G342 (= G347), S352 (≠ W357), V354 (≠ Y359), M356 (= M361), F359 (≠ Y364), M375 (= M380)
binding ubiquinone-10: C21 (= C44), L34 (= L57), P39 (= P62), P81 (= P104), L129 (= L152), W132 (= W155), E168 (≠ T190), R173 (= R195), I197 (≠ L217), D241 (≠ E261)

Sites not aligning to the query:

binding heme c: 428

7w2jC Cryo-em structure of membrane-bound fructose dehydrogenase from gluconobacter japonicus
39% identity, 91% coverage: 29:400/410 of query aligns to 1:386/418 of 7w2jC

query
sites
7w2jC

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D

A

V

V

A

K

K

Q

V

V

R

R

binding heme c: C13 (= C41), C16 (= C44), H17 (= H45), T42 (= T69), I44 (= I71), Y55 (= Y82), L75 (= L102), Y76 (= Y103), A78 (= A105), M79 (= M106), R122 (= R149), H161 (= H187), C162 (= C188), C165 (= C191), H166 (= H192), A191 (= A222), P192 (≠ G223), R223 (≠ A254), P227 (= P258), M228 (= M259), V289 (≠ N306), C290 (= C307), C293 (= C310), H294 (= H311), Y305 (≠ V321), Y306 (≠ V322), P307 (= P323), L309 (= L325), N312 (≠ S328), T313 (≠ S329), T314 (≠ A330), D322 (≠ S338), I327 (≠ V343), V331 (≠ G347), R333 (≠ N349), I340 (≠ Y359), M342 (= M361), P343 (= P362), F345 (≠ Y364)

8jejC Cryo-em structure of na-dithionite reduced membrane-bound fructose dehydrogenase from gluconobacter japonicus
37% identity, 91% coverage: 29:400/410 of query aligns to 1:400/413 of 8jejC

query
sites
8jejC

V

I

R

S

R

R

G

G

A

H

Y

Y

L

L

A

A

R

I

V

A

S

A

D

D

C
|
C

I

A

A

A

C
|
C

H
|
H

S

T

-

N

A

G

P

R

G

D

G

G

K

Q

E

F

N

L

A

A

G

G

L

Y

P

A

M

I

D

S

T

S

P

P

V
x
M

G

G

K

N

I

I

Y

Y

S

S

T
|
T

N

N

I
|
I

T

T

P

P

D

S

K

K

E

T

T

H

G

G

I

I

G

G

N

N

Y

Y

S

T

Y

L

E

E

D

Q

F
|
F

A

S

R

K

A

A

L
|
L

R

R

R

H

G

G

V

I

A

R

K

A

E

D

G

G

H

A

S

Q

L
|
L

Y
|
Y

P
|
P

A

A

M
|
M

P
|
P

Y

Y

T

D

S

A

F
x
Y

A

N

R

R

I

L

N

T

D

D

D

E

D

D

M

V

R

K

D

S

L

L

Y

Y

A

A

Y

Y

F

I

M

M

H

T

G

E

V

V

A

K

P

P

V

V

K

D

Q

A

A

P

N

S

R

P

D

K

S

T

D

Q

I

L

P

P

W

F

P

P

L

F

S

S

M

I

R
|
R

W

A

P
x
S

L

L

T

G

L

I

W
|
W

R

K

W

I

M

A

F

A

H

R

D

I

D

E

S

G

R

K

-

P

Y

Y

Q

V

P

F

D

D

A

H

A

T

K

H

N

N

A

D

E

D

W

W

N

N

R

R

G

G

A

R

Y

Y

L

L

V

V

Q

D

G

E

M

L

A

A

H

H

C
|
C

G

G

T

E

C
|
C

H
|
H

T

T

P

P

R

R

G

-

V

-

A

-

F

-

Q

-

E

N

V

F

A

L

L
|
L

S

A

E

P

K

N

K

Q

P

S

G

A

Y

Y

L

L

T

A

G

G

A

A

Q

D

L
x
I

A

G

G

S

W
|
W

Y

R

A
|
A

G
x
P

N

N

L
x
I

T

T

G

N

D

A

A

P

R

Q

E

S

G

G

L

I

G

G

G

S

W
|
W

S

S

A

D

D

Q

E

D

L

L

V

F

Q

Q

Y
|
Y

L

L

Q

K

T

T

G

G

R

K

N

T

R

A

Y

H

T

A

A
x
R

A

A

F

A

G

G

P

P

M
|
M

A

A

E

E

V

A

V

I

H

E

Y

H

S

S

S

L

Q

Q

H

Y

I

L

N

P

T

D

A

A

D

D

L

I

S

S

A

A

I

I

A

V

V

T

Y

Y

L

L

K

R

S

S

L

V

P

P

G

A

A

K

G

A

A

E

-

S

-

G

-

Q

-

T

-

V

-

A

-

N

-

F

-

E

-

H

-

A

-

G

-

R

-

P

-

S

-

Y

-

S

-

V

-

A

-

N

-

A

-

N

-

S

-

R

-

S

-

N

A

S

D

T

V

L

A

T

P

K

V

T

R

T

T

D

G

G

A

A

T

A

L

L

F

Y

F

E

E

A

N
x
V

C
|
C

A

A

T

S

C
|
C

H
|
H

Q

Q

A

S

N

D

G

G

Q

K

G

G

-

S

H

K

D

D

K

G

V

Y

V
x
Y

P
|
P

A

S

L
|
L

A

V

G

G

S

N

S
x
T

A
x
T

V

T

M

G

A

Q

R

L

D

N

A

P

T

N

S
x
D

L

L

I

I

R

A

V

S

V
x
I

L

L

E

Y

G

G

G
x
V

V

D

N
x
R

A

T

R

T

T

D

R

N

H

H

L

-

A

-

W

-

E

E

Y
x
I

S

L

M
|
M

P

P

G

A

Y
x
F

-

G

-

P

-

D

-

S

-

L

A

V

Q

Q

Q

P

M

L

N

T

D

D

Q

E

E

Q

V

I

A

A

D

T

V
x
I

L

A

T

D

Y

Y

M

V

R

L

Q

S

S

H

W

F

G

G

N

N

A

A

A

Q

S

A

P

T

V

V

A

S

A

A

G

D

D

A

V

V

A

K

K

Q

V

V

R

R

binding heme c: C13 (= C41), C16 (= C44), H17 (= H45), T42 (= T69), I44 (= I71), F60 (= F87), L64 (= L91), L75 (= L102), Y76 (= Y103), M79 (= M106), P80 (= P107), Y84 (≠ F111), R122 (= R149), C162 (= C188), C165 (= C191), H166 (= H192), I186 (≠ L217), W189 (= W220), A191 (= A222), P192 (≠ G223), I194 (≠ L225), W205 (= W236), Y213 (= Y244), R223 (≠ A254), M228 (= M259), V303 (≠ N306), C304 (= C307), C307 (= C310), H308 (= H311), Y320 (≠ V322), P321 (= P323), L323 (= L325), T327 (≠ S329), T328 (≠ A330), D336 (≠ S338), I341 (≠ V343), V345 (≠ G347), R347 (≠ N349), I354 (≠ Y359), M356 (= M361), F359 (≠ Y364), I376 (≠ V376)
binding ubiquinone-10: M36 (≠ V63), P77 (= P104), S124 (≠ P151), W128 (= W155), C165 (= C191), L173 (= L204)

Sites not aligning to the query:

binding ubiquinone-10: 408, 411

8gy3A Cryo-em structure of membrane-bound aldehyde dehydrogenase from gluconobacter oxydans
34% identity, 90% coverage: 13:383/410 of query aligns to 25:410/440 of 8gy3A

query
sites
8gy3A

L

I

A

A

C

P

A

I

A

P

N

R

A

P

A

A

L

A

A

S

H

S

V

F

D

S

T

A

D

D

G

A

V

I

R

S

R

R

G

G

A

E

Y

I

L

V

A

A

R

N

V

G

S

G

D
x
Y

C
|
C

I

A

A
x
E

C
|
C

H
|
H

S

T

A

R

P

V

G

D

G

G

K

K

-

P

-

G

-

P

E

E

N

L

A

A

G

G

G

D

L

F

P

K

M

M

D

A

T
|
T

P

P

V
x
F

G

G

K

D

I

I

Y

F

S

S

T
x
S

N

N

I
|
I

T

T

P

P

D

D

K

E

E

E

T

W

G

G

I

I

G

G

N

N

Y
x
W

S

S

Y

L

E

A

D

A

F
|
F

A

K

R

R

A

A

L

M

R

N

R

K

G

G

V

I

A

A

K

R

E

D

G

G

H

S

S

Q

L
|
L

Y
|
Y

P
|
P

A

A

M
x
F

P

P

Y

F

T

D

S

H

F
|
F

A

T

R

K

I

V

N

S

D

D

D

Q

D

D

M

V

R

S

D

D

L

L

Y

Y

A

A

Y

Y

F

L

M

M

H

T

G

R

V

P

A

A

P

-

V

V

K

H

Q

L

A

K

N

P

R

R

D

D

S

N

D

T

I

V

P

P

W

F

P

P

L
x
I

S

N

M

I

R
|
R

W

L

P

I

-
x
G

L
x
Q

T

G

L
x
F

W
|
W

R

K

W

L

M

L

F

F

H

F

D

T

D

P

S

G

R

R

Y

Y

Q

Q

P

N

D

D

A

P

A

K

K

H

N

D

A

A

E

Q

W

W

N

N

R

R

G

G

A

A

Y

Y

L

L

V

A

Q

E

G

G

M

N

A

E

H
|
H

C
|
C

G

G

T

A

C
|
C

H
|
H

T

T

P

P

R
|
R

G

N

V

L

A
x
L

F

G

Q

-

E

-

V

-

A

-

L

-

S

A

E

E

K

K

K

M

P

S

G

S

Y

V

L

Y

T

D

G

G

A

A

Q

V

L

I

A

D

G

G

W

W

Y

I

A
|
A

G
x
P

N

P

L
|
L

T

N

G

-

D

D

A

H

R

N

E

P

G

T

L

P

G

V

W
|
W

S

T

A

E

D

D

E

E

L

L

V

F

Q

Q

Y
|
Y

L
|
L

Q

R

T

F

G

G

-

V

R

A

N

P

R

L

Y

H

T
x
G

A
x
S

A

A

F

A

G

G

P

P

M
|
M

A

S

E

P

V

V

V

P

H

H

Y

R

S

F

S

L

Q

S

H

K

I

I

N

P

T

E

A

E

D

D

L

V

S

H

A

A

I

I

A

A

V

H

Y

Y

L
x
Y

K

A

S

D

L

V

P

D

G

K

A

A

G

A

-

Q

-

R

-

S

A

S

A

G

D

D

V

Q

A

A

-

A

-

I

-

T

-

R

-

A

-

M

-

Q

-

M

-

S

-

G

-

R

-

D

-

L

-

T

-

G

-

P

-

Q

P

P

V

L

R

D

T

E

G

D

A

A

T

R

L

L

F

Y

F

Q

E

G

N

A

C
|
C

A

G

T

A

C
|
C

H
|
H

Q

Y

A

N

N

S

G

G

Q

P

G

N

H

P

D

V

K

L

V

G

V

R

P
|
P

A

E

L
|
L

A

A

G

L

S
x
N

A

A

V

L

M

W

A

L

R

D

D

E

A

P

T

N

S

N

L

L

I

Y

R

Q

V
|
V

V

M

L

L

E

H

G

G

G
x
I

V

T

N

A

A

E

R

E

T
x
G

R
x
Q

H

D

L

-

A

-

W

-

E

H

Y
x
I

S

S

M
|
M

P

P

G

S

Y
x
F

A

Y

Q

S

Q

G

M

L

N

S

D

D

Q

H

E

D

V

M

A

A

D

R

V

I

L

A

T

A

Y

Y

M

L

R

R

Q

R

S

T

binding heme c: Y52 (≠ D40), C53 (= C41), C56 (= C44), H57 (= H45), S84 (≠ T69), I86 (= I71), W97 (≠ Y82), F102 (= F87), L117 (= L102), F121 (≠ M106), F126 (= F111), R163 (= R149), C203 (= C188), C206 (= C191), H207 (= H192), A232 (= A222), P233 (≠ G223), L235 (= L225), W245 (= W236), Y253 (= Y244), L254 (= L245), G263 (≠ T253), S264 (≠ A254), M269 (= M259), Y292 (≠ L282), C337 (= C307), C340 (= C310), H341 (= H311), P353 (= P323), L355 (= L325), N358 (≠ S328), N359 (≠ S329), V372 (= V342), I377 (≠ G347), G382 (≠ T352), Q383 (≠ R353), I386 (≠ Y359), M388 (= M361), F391 (≠ Y364)
binding ubiquinone-10: E55 (≠ A43), T76 (= T61), F78 (≠ V63), Y118 (= Y103), P119 (= P104), I160 (≠ L146), G166 (vs. gap), Q167 (≠ L152), F169 (≠ L154), W170 (= W155), H202 (= H187), R210 (= R195), L213 (≠ A198)

5oboA Crystal structure of nitrite bound d97n mutant of three-domain heme-cu nitrite reductase from ralstonia pickettii
42% identity, 26% coverage: 296:400/410 of query aligns to 350:453/456 of 5oboA

query
sites
5oboA

V

V

R

Q

T

A

G

G

A

R

T

A

L

L

F

F

F

A

E

G

N
x
T

C
|
C

A

S

T

V

C
|
C

H
|
H

Q

Q

A

G

N

N

G

G

Q

A

G

G

H

L

D

P

K

G

V

V

V

F

P
|
P

A
x
P

L
|
L

A

A

G

-

S

K

S
|
S

A

D

V
x
F

M

L

A

A

R

A

D

D

A

P

T

K

S

R

L

A

I

M

R

N

V
x
I

V

V

L

L

E

H

G

G

G

-

V

L

N
|
N

A
x
G

R

K

T

I

R

K

H

V

L

N

A

G

W

Q

E

E

Y

Y

S

D

-
x
S

-

V

M
|
M

P

P

G

P

Y
x
M

A

T

Q

Q

Q

-

M

L

N

N

D

D

Q

D

E

E

V

V

A

A

D

N

V

I

L

L

T

T

Y

Y

M

V

R

L

Q

N

S

S

W

W

G

D

N

N

A

P

A

G

S

G

P

R

V

V

A

S

A

A

G

E

D

D

V

V

A

K

K

K

V

V

R

R

binding heme c: T360 (≠ N306), C361 (= C307), C364 (= C310), H365 (= H311), P377 (= P323), P378 (≠ A324), L379 (= L325), S382 (= S329), F384 (≠ V331), I395 (≠ V342), N401 (= N349), G402 (≠ A350), S413 (vs. gap), M415 (= M361), M418 (≠ Y364)

Sites not aligning to the query:

active site: 91, 94, 96, 131, 132, 140, 145, 237, 259, 260, 286
binding copper (ii) ion: 91, 96, 131, 132, 140, 145
binding nitrite ion: 94, 96, 131

6fjaA Crystal structure of t2d three-domain heme-cu nitrite reductase from ralstonia pickettii
42% identity, 26% coverage: 296:400/410 of query aligns to 349:452/455 of 6fjaA

query
sites
6fjaA

V

V

R

Q

T

A

G

G

A

R

T

A

L

L

F

F

F

A

E

G

N
x
T

C
|
C

A

S

T

V

C
|
C

H
|
H

Q

Q

A

G

N

N

G

G

Q

A

G

G

H

L

D

P

K

G

V

V

V

F

P
|
P

A
x
P

L
|
L

A

A

G

-

S

K

S

S

A

D

V
x
F

M

L

A

A

R

A

D

D

A

P

T

K

S

R

L

A

I

M

R

N

V

I

V

V

L

L

E

H

G

G

G

-

V

L

N
|
N

A
x
G

R

K

T

I

R

K

H

V

L

N

A

G

W

Q

E

E

Y
|
Y

S

D

-
x
S

-

V

M
|
M

P

P

G

P

Y
x
M

A

T

Q

Q

Q

-

M

L

N

N

D

D

Q

D

E

E

V

V

A

A

D

N

V

I

L

L

T

T

Y

Y

M

V

R

L

Q

N

S

S

W

W

G

D

N

N

A

P

A

G

S

G

P

R

V

V

A

S

A

A

G

E

D

D

V

V

A

K

K

K

V

V

R

R

binding protoporphyrin ix containing fe: T359 (≠ N306), C360 (= C307), C363 (= C310), H364 (= H311), P376 (= P323), P377 (≠ A324), L378 (= L325), F383 (≠ V331), N400 (= N349), G401 (≠ A350), Y410 (= Y359), S412 (vs. gap), M414 (= M361), M417 (≠ Y364)

Sites not aligning to the query:

active site: 90, 93, 95, 130, 131, 139, 144, 236, 258, 259, 285
binding copper (ii) ion: 90, 131, 139, 144

4ax3D Structure of three-domain heme-cu nitrite reductase from ralstonia pickettii at 1.6 a resolution (see paper)
42% identity, 26% coverage: 296:400/410 of query aligns to 352:455/457 of 4ax3D

query
sites
4ax3D

V

V

R

Q

T

A

G

G

A

R

T

A

L

L

F

F

F

A

E

G

N

T

C
|
C

A

S

T

V

C
|
C

H
|
H

Q

Q

A

G

N

N

G

G

Q

A

G

G

H

L

D

P

K

G

V

V

V

F

P
|
P

A
x
P

L
|
L

A

A

G

-

S

K

S
|
S

A

D

V
x
F

M

L

A

A

R

A

D

D

A

P

T

K

S

R

L

A

I

M

R

N

V

I

V

V

L

L

E

H

G

G

G

-

V

L

N
|
N

A
x
G

R

K

T

I

R

K

H

V

L

N

A

G

W

Q

E

E

Y

Y

S

D

-
x
S

-

V

M
|
M

P

P

G

P

Y
x
M

A

T

Q

Q

Q

-

M

L

N

N

D

D

Q

D

E

E

V

V

A

A

D

N

V

I

L

L

T

T

Y

Y

M

V

R

L

Q

N

S

S

W

W

G

D

N

N

A

P

A

G

S

G

P

R

V

V

A

S

A

A

G

E

D

D

V

V

A

K

K

K

V

V

R

R

binding heme c: C363 (= C307), C366 (= C310), H367 (= H311), P379 (= P323), P380 (≠ A324), L381 (= L325), S384 (= S329), F386 (≠ V331), N403 (= N349), G404 (≠ A350), S415 (vs. gap), M417 (= M361), M420 (≠ Y364)

Sites not aligning to the query:

active site: 93, 96, 98, 133, 134, 142, 147, 239, 261, 262, 288
binding copper (ii) ion: 93, 98, 133, 134, 142, 147

8hddB Complex structure of catalytic, small, and a partial electron transfer subunits from burkholderia cepacia fad glucose dehydrogenase (see paper)
35% identity, 25% coverage: 300:400/410 of query aligns to 23:121/121 of 8hddB

query
sites
8hddB

A

A

T

R

L

L

F

Y

F

L

E

G

N

N

C
|
C

A

A

T

T

C
|
C

H
|
H

Q

Q

A

M

N

Q

G

G

Q

K

G

G

H

T

-

P

D

D

K

G

V

Y

V
x
Y

P
|
P

A

S

L

L

A

F

G

H

S

N

S

S

A
x
T

V

V

M

G

A

A

R

S

D

N

A

P

T

S

S

N

L

L

I

V

R

Q

V
|
V

V
x
I

L

L

E

N

G

G

G

-

V

-

N

-

A

V

R

Q

T
x
R

R

K

H

I

L

G

A

S

W

E

E

D

Y
x
I

S

G

M
|
M

P
|
P

G

A

Y

F

A

R

Q

Y

Q

D

M

L

N

N

D

D

Q

A

E

Q

V

I

A

A

D

A

V

L

L

T

T

N

Y

Y

M

V

R

T

Q

A

S

Q

W

F

G

G

N

N

A

P

A

A

S

A

P

K

V

V

A

T

A

E

G

Q

D

D

V

V

A

A

K

K

V

L

R

R

binding protoporphyrin ix containing fe: C30 (= C307), C33 (= C310), H34 (= H311), Y46 (≠ V322), P47 (= P323), T54 (≠ A330), V66 (= V342), I67 (≠ V343), R73 (≠ T352), I80 (≠ Y359), M82 (= M361), P83 (= P362)

2zooA Crystal structure of nitrite reductase from pseudoalteromonas haloplanktis tac125
32% identity, 26% coverage: 296:400/410 of query aligns to 336:438/438 of 2zooA

query
sites
2zooA

V

I

R

R

T

F

G

G

A

Q

T

R

L

V

F

Y

F

E

E

A

N

N

C
|
C

A

M

T

A

C
|
C

H
|
H

Q

Q

A

A

N

N

G

G

Q

E

G

G

H

I

D

P

K

G

V

A

V
x
F

P
|
P

A
x
P

L
|
L

A

A

G

K

S
|
S

S

D

A
x
Y

V

L

M

N

A

N

R

N

D

P

A

L

T

L

S

G

L

V

I

N

R

A

V
x
I

V

I

-

K

-

G

L
|
L

E
x
S

G
|
G

G

P

V
x
I

N

-

A

-

R

K

T
x
V

R

N

H

N

L

V

A

N

W
x
Y

E
x
N

Y
x
G

S
x
V

M
|
M

P

P

G

-

Y

-

A

A

Q

M

Q

N

M

L

N

N

D

D

Q

E

E

D

V

I

A

A

D

N

V

V

L

I

T

T

Y

F

M

V

R

L

Q

N

S

N

W

W

G

D

N

N

A

A

A

G

S

G

P

K

V

V

A

S

A

A

G

E

D

Q

V

V

A

A

K

K

V

Q

R

R

binding protoporphyrin ix containing fe: C347 (= C307), C350 (= C310), H351 (= H311), F362 (≠ V322), P363 (= P323), P364 (≠ A324), L365 (= L325), S368 (= S328), Y370 (≠ A330), I382 (≠ V342), L386 (= L344), S387 (≠ E345), G388 (= G346), I390 (≠ V348), V392 (≠ T352), Y397 (≠ W357), N398 (≠ E358), G399 (≠ Y359), V400 (≠ S360), M401 (= M361)

Sites not aligning to the query:

active site: 81, 84, 86, 121, 122, 130, 135, 227, 249, 250, 276
binding copper (ii) ion: 81, 86, 121, 122, 130, 135

1dt1A Thermus thermophilus cytochrome c552 synthesized by escherichia coli (see paper)
34% identity, 24% coverage: 304:401/410 of query aligns to 6:111/129 of 1dt1A

query
sites
1dt1A

F

Y

E

A

N

Q

C
|
C

A

A

T

G

C
|
C

H
|
H

Q

Q

A

Q

N

N

G

G

Q

Q

G

G

H

I

D

P

K

G

V

A

V

F

P
|
P

A

P

L

L

A

A

G

G

S
x
H

S

V

A

A

-

E

V

I

M

L

A

A

R

K

D

E

A

G

T

G

S

R

-

E

-
x
Y

L

L

I

I

R

L

V
|
V

V

L

L

L

E

Y

G

G

-

L

G
x
Q

V
x
G

N

Q

A

I

R

E

T

V

R

K

H

G

L

M

A

K

W

Y

E

N

Y
x
G

S

V

M
|
M

P

S

G

S

Y
x
F

A

A

Q

-

Q

Q

M

L

N

K

D

D

Q

E

E

E

V

I

A

A

D

A

V

V

L

L

T

N

Y

H

M

I

R

A

Q

T

S

A

W

W

G

G

N

D

A

A

A

K

-

K

-

V

-

K

-

G

-

F

S

K

P

P

V

F

A

T

A

A

G

E

D

E

V

V

A

K

K

K

V

L

R

R

G

A

binding heme c: C9 (= C307), C12 (= C310), H13 (= H311), P25 (= P323), H30 (≠ S328), Y43 (vs. gap), V47 (= V342), Q53 (≠ G347), G54 (≠ V348), G65 (≠ Y359), M67 (= M361), F70 (≠ Y364)

Sites not aligning to the query:

binding heme c: 123

1r0qA Characterization of the conversion of the malformed, recombinant cytochrome rc552 to a 2-formyl-4-vinyl (spirographis) heme (see paper)
34% identity, 24% coverage: 304:401/410 of query aligns to 7:112/130 of 1r0qA

query
sites
1r0qA

F

Y

E

A

N

Q

C

C

A

A

T

G

C
|
C

H
|
H

Q

Q

A

Q

N

N

G

G

Q

Q

G

G

H

I

D

P

K

G

V

A

V

F

P
|
P

A

P

L
|
L

A

A

G

G

S
x
H

S

V

A

A

-

E

V

I

M

L

A

A

R

K

D

E

A

G

T

G

S

R

-

E

-
x
Y

L

L

I

I

R

L

V
|
V

V

L

L

L

E

Y

G

G

-

L

G
x
Q

V
x
G

N

Q

A

I

R

E

T

V

R

K

H

G

L

M

A

K

W

Y

E

N

Y
x
G

S

V

M
|
M

P

S

G

S

Y

F

A

A

Q

-

Q

Q

M

L

N

K

D

D

Q

E

E

E

V

I

A

A

D

A

V

V

L

L

T

N

Y

H

M

I

R

A

Q

T

S

A

W

W

G

G

N

D

A

A

A

K

-

K

-

V

-

K

-

G

-

F

S

K

P

P

V

F

A

T

A

A

G

E

D

E

V

V

A

K

K

K

V

L

R

R

G

A

binding 2-formyl-protoporphryn ix: C13 (= C310), H14 (= H311), P26 (= P323), L28 (= L325), H31 (≠ S328), Y44 (vs. gap), V48 (= V342), Q54 (≠ G347), G55 (≠ V348), G66 (≠ Y359), M68 (= M361)

Sites not aligning to the query:

binding 2-formyl-protoporphryn ix: 124

1qyzA Characterization of the malformed, recombinant cytochrome rc552 (see paper)
34% identity, 24% coverage: 304:401/410 of query aligns to 7:112/130 of 1qyzA

query
sites
1qyzA

F
x
Y

E

A

N

Q

C
|
C

A

A

T

G

C
|
C

H
|
H

Q

Q

A

Q

N

N

G

G

Q

Q

G

G

H

I

D

P

K

G

V

A

V

F

P
|
P

A

P

L

L

A

A

G

G

S
x
H

S

V

A

A

-

E

V

I

M

L

A

A

R

K

D

E

A

G

T

G

S

R

-

E

-
x
Y

L

L

I

I

R

L

V

V

V

L

L

L

E

Y

G

G

-

L

G
x
Q

V
x
G

N

Q

A

I

R

E

T

V

R

K

H

G

L

M

A

K

W

Y

E

N

Y
x
G

S

V

M
|
M

P

S

G

S

Y
x
F

A

A

Q

-

Q

Q

M

L

N

K

D

D

Q

E

E

E

V

I

A

A

D

A

V

V

L

L

T

N

Y

H

M

I

R

A

Q

T

S

A

W

W

G

G

N

D

A

A

A

K

-

K

-

V

-

K

-

G

-

F

S

K

P

P

V

F

A

T

A

A

G

E

D

E

V

V

A

K

K

K

V

L

R

R

G

A

binding 2-acetyl-protoporphyrin ix: Y7 (≠ F304), C10 (= C307), C13 (= C310), H14 (= H311), P26 (= P323), H31 (≠ S328), Y44 (vs. gap), Q54 (≠ G347), G55 (≠ V348), G66 (≠ Y359), M68 (= M361), F71 (≠ Y364)

Sites not aligning to the query:

binding 2-acetyl-protoporphyrin ix: 124

1c52A Thermus thermophilus cytochrome-c552: a new highly thermostable cytochromE-C structure obtained by mad phasing (see paper)
34% identity, 24% coverage: 304:401/410 of query aligns to 8:113/131 of 1c52A

query
sites
1c52A

F

Y

E

A

N

Q

C
|
C

A

A

T

G

C
|
C

H
|
H

Q

Q

A

Q

N

N

G

G

Q

Q

G

G

H

I

D

P

K

G

V

A

V
x
F

P
|
P

A

P

L
|
L

A

A

G

G

S
x
H

S

V

A

A

-

E

V

I

M

L

A

A

R

K

D

E

A

G

T

G

S

R

-

E

-
x
Y

L

L

I

I

R

L

V

V

V

L

L

L

E

Y

G

G

-
x
L

G
x
Q

V
x
G

N

Q

A

I

R

E

T

V

R

K

H

G

L

M

A

K

W

Y

E

N

Y
x
G

S

V

M
|
M

P

S

G

S

Y
x
F

A

A

Q

-

Q

Q

M

L

N

K

D

D

Q

E

E

E

V

I

A

A

D

A

V

V

L

L

T

N

Y

H

M

I

R

A

Q

T

S

A

W

W

G

G

N

D

A

A

A

K

-

K

-

V

-

K

-

G

-

F

S

K

P

P

V

F

A

T

A

A

G

E

D

E

V

V

A

K

K

K

V

L

R

R

G

A

binding protoporphyrin ix containing fe: C11 (= C307), C14 (= C310), H15 (= H311), F26 (≠ V322), P27 (= P323), L29 (= L325), H32 (≠ S328), Y45 (vs. gap), L54 (vs. gap), Q55 (≠ G347), G56 (≠ V348), G67 (≠ Y359), M69 (= M361), F72 (≠ Y364)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 125

Query Sequence

>WP_046158324.1 NCBI__GCF_000971335.1:WP_046158324.1
MKLMKTLGLAALLACAANAALAHVDTDGVRRGAYLARVSDCIACHSAPGGKENAGGLPMD
TPVGKIYSTNITPDKETGIGNYSYEDFARALRRGVAKEGHSLYPAMPYTSFARINDDDMR
DLYAYFMHGVAPVKQANRDSDIPWPLSMRWPLTLWRWMFHDDSRYQPDAAKNAEWNRGAY
LVQGMAHCGTCHTPRGVAFQEVALSEKKPGYLTGAQLAGWYAGNLTGDAREGLGGWSADE
LVQYLQTGRNRYTAAFGPMAEVVHYSSQHINTADLSAIAVYLKSLPGAGAADVAPVRTGA
TLFFENCATCHQANGQGHDKVVPALAGSSAVMARDATSLIRVVLEGGVNARTRHLAWEYS
MPGYAQQMNDQEVADVLTYMRQSWGNAASPVAAGDVAKVRGIVADDAHQK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory