SitesBLAST
Comparing WP_046158449.1 NCBI__GCF_000971335.1:WP_046158449.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P9WIT1 Uncharacterized FAD-linked oxidoreductase Rv2280; EC 1.-.-.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
25% identity, 52% coverage: 41:525/938 of query aligns to 39:459/459 of P9WIT1
- K354 (≠ I408) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
Q8N465 D-2-hydroxyglutarate dehydrogenase, mitochondrial; EC 1.1.99.39 from Homo sapiens (Human) (see 7 papers)
25% identity, 54% coverage: 9:518/938 of query aligns to 67:513/521 of Q8N465
- S109 (≠ E51) to W: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs142050154
- N127 (≠ T69) to K: in D2HGA1; uncertain significance; complete loss of catalytic activity; dbSNP:rs762857195
- G131 (≠ A73) to V: in D2HGA1; uncertain significance; complete loss of catalytic activity
- I147 (≠ L89) to S: in D2HGA1; uncertain significance; severe phenotype; almost complete loss of catalytic activity; dbSNP:rs121434361
- M153 (≠ F94) to T: in D2HGA1; uncertain significance; significant loss of catalytic activity; to V: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs1432270139
- C172 (≠ A119) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs773735172
- P189 (vs. gap) to L: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs587783517
- A205 (= A145) to V: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs750889931
- A231 (≠ L171) to V: in D2HGA1; uncertain significance; significant loss of catalytic activity
- G233 (= G173) to S: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs374535734
- D375 (= D371) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs267606759
- R386 (= R382) binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- T390 (≠ F386) binding (R)-2-hydroxyglutarate; binding (R)-malate; mutation to A: Significantly reduced catalytic activity.
- V399 (= V399) to M: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746519212
- K401 (≠ I401) binding (R)-2-hydroxyglutarate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- R419 (≠ T419) to H: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs199908032
- A426 (≠ G426) to T: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs146578303
- H434 (= H435) binding Zn(2+); mutation to A: Loss of catalytic activity.
- G436 (≠ L437) to V: slight reduction in catalytic activity
- N439 (= N440) to D: in D2HGA1; uncertain significance; mild phenotype; moderate reduction in catalytic activity; dbSNP:rs121434362
- H441 (= H442) binding Zn(2+); mutation to A: Loss of catalytic activity.
- N443 (≠ V444) binding (R)-2-hydroxyglutarate; mutation to A: Significantly reduced catalytic activity.
- V444 (≠ F445) to A: in D2HGA1; uncertain significance; severe phenotype; significant reduction in catalytic activity; dbSNP:rs121434360
- A446 (≠ P447) to V: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746956176
- E475 (= E480) binding Zn(2+); mutation to A: Loss of catalytic activity.
- H476 (= H481) binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- G477 (= G482) to R: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs1453924640
Sites not aligning to the query:
- 15 R → G: in dbSNP:rs4675887
- 169:521 natural variant: Missing (in D2HGA1; uncertain significance)
- 400:521 natural variant: Missing (in D2HGA1; uncertain significance; complete loss of catalytic activity)
6lpnB Crystal structure of human d-2-hydroxyglutarate dehydrogenase in apo form (see paper)
25% identity, 54% coverage: 9:518/938 of query aligns to 15:461/467 of 6lpnB
- binding flavin-adenine dinucleotide: W40 (≠ A35), P76 (≠ F70), G78 (≠ A72), G79 (≠ A73), N80 (≠ G74), T81 (= T75), G82 (≠ S76), M83 (≠ L77), G86 (≠ Q80), S87 (≠ A81), L140 (≠ P132), A142 (≠ S134), C146 (≠ A138), H147 (≠ K139), G150 (= G142), N151 (≠ I143), A153 (= A145), T154 (≠ N146), G208 (= G251), I212 (≠ F255), I213 (= I256), E423 (= E480), N460 (= N517)
6lpxA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with 2-oxoglutarate (2-og) (see paper)
25% identity, 54% coverage: 9:518/938 of query aligns to 14:460/466 of 6lpxA
- binding 2-oxoglutaric acid: R333 (= R382), T337 (≠ F386), K348 (≠ I401), Y379 (≠ F433), H381 (= H435), H388 (= H442), H423 (= H481)
- binding flavin-adenine dinucleotide: W39 (≠ A35), P75 (≠ F70), Q76 (≠ R71), G77 (≠ A72), G78 (≠ A73), N79 (≠ G74), T80 (= T75), G81 (≠ S76), M82 (≠ L77), G85 (≠ Q80), S86 (≠ A81), L139 (≠ P132), G140 (≠ A133), A141 (≠ S134), C145 (≠ A138), G149 (= G142), N150 (≠ I143), A152 (= A145), T153 (≠ N146), G157 (= G150), G207 (= G251), I212 (= I256), E422 (= E480), N459 (= N517)
- binding zinc ion: H381 (= H435), H388 (= H442), E422 (= E480)
6lpuA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with l-2-hydroxyglutarate (l-2-hg) (see paper)
25% identity, 54% coverage: 9:518/938 of query aligns to 14:460/466 of 6lpuA
- binding flavin-adenine dinucleotide: W39 (≠ A35), P75 (≠ F70), G77 (≠ A72), G78 (≠ A73), N79 (≠ G74), T80 (= T75), G81 (≠ S76), G85 (≠ Q80), S86 (≠ A81), L139 (≠ P132), G140 (≠ A133), A141 (≠ S134), C145 (≠ A138), H146 (≠ K139), G148 (= G141), G149 (= G142), N150 (≠ I143), A152 (= A145), T153 (≠ N146), A155 (≠ S148), E206 (= E250), G207 (= G251), I211 (≠ F255), I212 (= I256), E422 (= E480), N459 (= N517)
- binding (2s)-2-hydroxypentanedioic acid: R333 (= R382), T337 (≠ F386), K348 (≠ I401), Y379 (≠ F433), H381 (= H435), H388 (= H442), H423 (= H481)
- binding zinc ion: H381 (= H435), H388 (= H442), E422 (= E480)
6lpqA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-malate (d-mal) (see paper)
25% identity, 54% coverage: 9:518/938 of query aligns to 14:460/466 of 6lpqA
- binding flavin-adenine dinucleotide: W39 (≠ A35), P75 (≠ F70), G77 (≠ A72), G78 (≠ A73), N79 (≠ G74), T80 (= T75), G81 (≠ S76), G85 (≠ Q80), S86 (≠ A81), L139 (≠ P132), G140 (≠ A133), A141 (≠ S134), C145 (≠ A138), H146 (≠ K139), G149 (= G142), N150 (≠ I143), A152 (= A145), T153 (≠ N146), A155 (≠ S148), G157 (= G150), E206 (= E250), G207 (= G251), I211 (≠ F255), I212 (= I256), E422 (= E480), N459 (= N517)
- binding d-malate: M82 (≠ L77), R333 (= R382), T337 (≠ F386), K348 (≠ I401), Y379 (≠ F433), H381 (= H435), H388 (= H442), E422 (= E480), H423 (= H481)
- binding zinc ion: H381 (= H435), H388 (= H442), E422 (= E480)
6lppA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-2-hydroxyglutarate (d-2-hg) (see paper)
25% identity, 54% coverage: 9:518/938 of query aligns to 14:460/466 of 6lppA
- binding (2R)-2-hydroxypentanedioic acid: R333 (= R382), T337 (≠ F386), K348 (≠ I401), Y379 (≠ F433), H381 (= H435), H388 (= H442), N390 (≠ V444), H423 (= H481)
- binding flavin-adenine dinucleotide: W39 (≠ A35), P75 (≠ F70), G77 (≠ A72), G78 (≠ A73), N79 (≠ G74), T80 (= T75), G81 (≠ S76), M82 (≠ L77), G85 (≠ Q80), S86 (≠ A81), L139 (≠ P132), G140 (≠ A133), A141 (≠ S134), C145 (≠ A138), G149 (= G142), N150 (≠ I143), A152 (= A145), T153 (≠ N146), A155 (≠ S148), G157 (= G150), G207 (= G251), I212 (= I256), E422 (= E480), H423 (= H481)
- binding zinc ion: H381 (= H435), H388 (= H442), E422 (= E480)
3pm9A Crystal structure of a putative dehydrogenase (rpa1076) from rhodopseudomonas palustris cga009 at 2.57 a resolution
25% identity, 55% coverage: 8:522/938 of query aligns to 6:465/465 of 3pm9A