SitesBLAST
Comparing WP_046158765.1 NCBI__GCF_000971335.1:WP_046158765.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3pm9A Crystal structure of a putative dehydrogenase (rpa1076) from rhodopseudomonas palustris cga009 at 2.57 a resolution
46% identity, 99% coverage: 3:458/460 of query aligns to 5:463/465 of 3pm9A
- active site: A149 (= A149), L159 (≠ T159)
- binding flavin-adenine dinucleotide: P69 (= P67), Q70 (= Q68), G71 (= G69), G72 (= G70), N73 (= N71), T74 (= T72), G75 (≠ S73), L76 (≠ T74), G79 (≠ A77), Q80 (≠ A78), L91 (= L91), L133 (= L133), G134 (≠ A134), A135 (≠ S135), C139 (= C139), T140 (≠ Q140), G142 (= G142), G143 (= G143), S146 (= S146), T147 (= T147), A149 (= A149), G150 (= G150), E200 (= E200), G201 (= G201), I205 (≠ L205), I206 (= I206), E423 (= E418)
6lpxA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with 2-oxoglutarate (2-og) (see paper)
37% identity, 99% coverage: 6:460/460 of query aligns to 12:464/466 of 6lpxA
- binding 2-oxoglutaric acid: R333 (= R323), T337 (≠ S327), K348 (= K338), Y379 (≠ F371), H381 (= H373), H388 (= H380), H423 (= H419)
- binding flavin-adenine dinucleotide: W39 (= W31), P75 (= P67), Q76 (= Q68), G77 (= G69), G78 (= G70), N79 (= N71), T80 (= T72), G81 (≠ S73), M82 (≠ T74), G85 (≠ A77), S86 (≠ A78), L139 (= L133), G140 (≠ A134), A141 (≠ S135), C145 (= C139), G149 (= G143), N150 (= N144), A152 (≠ S146), T153 (= T147), G157 (= G151), G207 (= G201), I212 (= I206), E422 (= E418), N459 (= N455)
- binding zinc ion: H381 (= H373), H388 (= H380), E422 (= E418)
6lpuA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with l-2-hydroxyglutarate (l-2-hg) (see paper)
37% identity, 99% coverage: 6:460/460 of query aligns to 12:464/466 of 6lpuA
- binding flavin-adenine dinucleotide: W39 (= W31), P75 (= P67), G77 (= G69), G78 (= G70), N79 (= N71), T80 (= T72), G81 (≠ S73), G85 (≠ A77), S86 (≠ A78), L139 (= L133), G140 (≠ A134), A141 (≠ S135), C145 (= C139), H146 (≠ Q140), G148 (= G142), G149 (= G143), N150 (= N144), A152 (≠ S146), T153 (= T147), A155 (= A149), E206 (= E200), G207 (= G201), I211 (≠ L205), I212 (= I206), E422 (= E418), N459 (= N455)
- binding (2s)-2-hydroxypentanedioic acid: R333 (= R323), T337 (≠ S327), K348 (= K338), Y379 (≠ F371), H381 (= H373), H388 (= H380), H423 (= H419)
- binding zinc ion: H381 (= H373), H388 (= H380), E422 (= E418)
6lpqA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-malate (d-mal) (see paper)
37% identity, 99% coverage: 6:460/460 of query aligns to 12:464/466 of 6lpqA
- binding flavin-adenine dinucleotide: W39 (= W31), P75 (= P67), G77 (= G69), G78 (= G70), N79 (= N71), T80 (= T72), G81 (≠ S73), G85 (≠ A77), S86 (≠ A78), L139 (= L133), G140 (≠ A134), A141 (≠ S135), C145 (= C139), H146 (≠ Q140), G149 (= G143), N150 (= N144), A152 (≠ S146), T153 (= T147), A155 (= A149), G157 (= G151), E206 (= E200), G207 (= G201), I211 (≠ L205), I212 (= I206), E422 (= E418), N459 (= N455)
- binding d-malate: M82 (≠ T74), R333 (= R323), T337 (≠ S327), K348 (= K338), Y379 (≠ F371), H381 (= H373), H388 (= H380), E422 (= E418), H423 (= H419)
- binding zinc ion: H381 (= H373), H388 (= H380), E422 (= E418)
6lppA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-2-hydroxyglutarate (d-2-hg) (see paper)
37% identity, 99% coverage: 6:460/460 of query aligns to 12:464/466 of 6lppA
- binding (2R)-2-hydroxypentanedioic acid: R333 (= R323), T337 (≠ S327), K348 (= K338), Y379 (≠ F371), H381 (= H373), H388 (= H380), N390 (= N382), H423 (= H419)
- binding flavin-adenine dinucleotide: W39 (= W31), P75 (= P67), G77 (= G69), G78 (= G70), N79 (= N71), T80 (= T72), G81 (≠ S73), M82 (≠ T74), G85 (≠ A77), S86 (≠ A78), L139 (= L133), G140 (≠ A134), A141 (≠ S135), C145 (= C139), G149 (= G143), N150 (= N144), A152 (≠ S146), T153 (= T147), A155 (= A149), G157 (= G151), G207 (= G201), I212 (= I206), E422 (= E418), H423 (= H419)
- binding zinc ion: H381 (= H373), H388 (= H380), E422 (= E418)
6lpnB Crystal structure of human d-2-hydroxyglutarate dehydrogenase in apo form (see paper)
37% identity, 99% coverage: 6:460/460 of query aligns to 13:465/467 of 6lpnB
- binding flavin-adenine dinucleotide: W40 (= W31), P76 (= P67), G78 (= G69), G79 (= G70), N80 (= N71), T81 (= T72), G82 (≠ S73), M83 (≠ T74), G86 (≠ A77), S87 (≠ A78), L140 (= L133), A142 (≠ S135), C146 (= C139), H147 (≠ Q140), G150 (= G143), N151 (= N144), A153 (≠ S146), T154 (= T147), G208 (= G201), I212 (≠ L205), I213 (= I206), E423 (= E418), N460 (= N455)
Q8N465 D-2-hydroxyglutarate dehydrogenase, mitochondrial; EC 1.1.99.39 from Homo sapiens (Human) (see 7 papers)
37% identity, 99% coverage: 6:460/460 of query aligns to 65:517/521 of Q8N465
- S109 (≠ T48) to W: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs142050154
- N127 (≠ V66) to K: in D2HGA1; uncertain significance; complete loss of catalytic activity; dbSNP:rs762857195
- G131 (= G70) to V: in D2HGA1; uncertain significance; complete loss of catalytic activity
- I147 (≠ V88) to S: in D2HGA1; uncertain significance; severe phenotype; almost complete loss of catalytic activity; dbSNP:rs121434361
- M153 (≠ L94) to T: in D2HGA1; uncertain significance; significant loss of catalytic activity; to V: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs1432270139
- C172 (≠ V113) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs773735172
- P189 (= P130) to L: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs587783517
- A205 (≠ S146) to V: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs750889931
- A231 (≠ P172) to V: in D2HGA1; uncertain significance; significant loss of catalytic activity
- G233 (= G174) to S: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs374535734
- D375 (≠ N312) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs267606759
- R386 (= R323) binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- T390 (≠ S327) binding (R)-2-hydroxyglutarate; binding (R)-malate; mutation to A: Significantly reduced catalytic activity.
- V399 (≠ S336) to M: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746519212
- K401 (= K338) binding (R)-2-hydroxyglutarate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- R419 (≠ A356) to H: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs199908032
- A426 (≠ G365) to T: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs146578303
- H434 (= H373) binding Zn(2+); mutation to A: Loss of catalytic activity.
- G436 (= G375) to V: slight reduction in catalytic activity
- N439 (= N378) to D: in D2HGA1; uncertain significance; mild phenotype; moderate reduction in catalytic activity; dbSNP:rs121434362
- H441 (= H380) binding Zn(2+); mutation to A: Loss of catalytic activity.
- N443 (= N382) binding (R)-2-hydroxyglutarate; mutation to A: Significantly reduced catalytic activity.
- V444 (= V383) to A: in D2HGA1; uncertain significance; severe phenotype; significant reduction in catalytic activity; dbSNP:rs121434360
- A446 (= A391) to V: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746956176
- E475 (= E418) binding Zn(2+); mutation to A: Loss of catalytic activity.
- H476 (= H419) binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- G477 (= G420) to R: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs1453924640
Sites not aligning to the query:
- 15 R → G: in dbSNP:rs4675887
- 169:521 natural variant: Missing (in D2HGA1; uncertain significance)
- 400:521 natural variant: Missing (in D2HGA1; uncertain significance; complete loss of catalytic activity)
P39976 D-2-hydroxyglutarate--pyruvate transhydrogenase DLD3; D-2HG--pyruvate transhydrogenase DLD3; (R)-2-hydroxyglutarate--pyruvate transhydrogenase; D-lactate dehydrogenase [cytochrome] 3; D-lactate ferricytochrome C oxidoreductase; D-LCR; EC 1.1.99.40; EC 1.1.2.4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
34% identity, 95% coverage: 25:460/460 of query aligns to 54:496/496 of P39976