SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_047005978.1 NCBI__GCF_001010925.1:WP_047005978.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q89WV5 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) (see paper)
67% identity, 97% coverage: 20:642/642 of query aligns to 22:645/648 of Q89WV5

query
sites
Q89WV5

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K

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G263 (= G260) mutation to I: Loss of activity.
G266 (= G263) mutation to I: Great decrease in activity.
K269 (= K266) mutation to G: Great decrease in activity.
E414 (= E411) mutation to Q: Great decrease in activity.

P27550 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Escherichia coli (strain K12) (see paper)
63% identity, 97% coverage: 16:640/642 of query aligns to 19:646/652 of P27550

query
sites
P27550

C

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Q

K609 (= K603) modified: N6-acetyllysine; by autocatalysis

Q8ZKF6 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
64% identity, 97% coverage: 17:640/642 of query aligns to 20:646/652 of Q8ZKF6

query
sites
Q8ZKF6

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G

-

N

V

V

D

S

I

I

K

K

W

W

F

Y

S

E

D

D

G

G

T

T

L

L

N

N

I

L

C

A

Y

A

N

N

A

C

V

L

D

D

R

R

H

H

L

L

D

Q

T

E

H

N

G

G

D

D

Q

R

I

T

A

A

L

I

I

I

F

W

E

E

P

G

D

D

S

D

P

-

D

T

A

S

P

Q

G

S

R

K

S

H

L

I

T

S

Y

Y

R

R

Q

E

L

L

H

H

K

R

E

D

V

V

T

C

R

R

V

F

A

A

N

N

A

T

L

L

K

L

A

D

M

L

G

G

V

I

R

K

K

K

G

G

D

D

R

V

V

V

T

A

L

I

Y

Y

M

M

P

P

M

M

I

V

V

P

E

E

G

A

A

A

L

V

T

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

I

V

H

H

S

S

V

V

V

I

F

F

G

G

F

F

S

S

P

P

E

E

A

A

L

V

A

A

G

G

R

R

I

I

M

I

A

D

C

S

E

S

S

S

R

R

F

L

V

V

V

I

C

T

A

A

D

D

Q

E

G

G

L

V

R

R

G

A

S

G

K
x
R

T

S

V

I

P

P

L

L

K

K

A

K

N

N

V

V

D

D

A

D

A

A

L

L

A

K

M

N

Q

P

G

N

V

V

-

T

S

S

V

V

D

E

G

H

V

V

L

I

V

V

F

L

D

K

H

R

T

T

G

G

G

S

E

D

I

I

E

D

M

W

V

Q

D

E

G

G

R

R

D

D

H

L

W

W

F

W

A

R

D

D

F

L

-

I

-

E

-

K

A

A

S

S

D

P

D

E

E

H

C

Q

P

P

C

-

E

E

E

A

M

M

N

N

A

A

E

E

D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T

T

S

S

G

G

S

S

T

T

G

G

T

K

P

P

K

K

G

G

V

V

L

L

H

H

T

T

G

G

Y

Y

A

L

L

V

W

Y

A

A

T

T

F

F

H

K

Y

Y

T

V

F

F

D

D

Y

Y

Q

H

P

P

G

G

D

D

I

I

F

Y

W

W

C

C

S

T

A

A

D

D

I

V

G

G

W

W

V

V

T
|
T

G

G

H

H

T

S

Y

Y

V

L

T

L

Y

Y

G

G

P

P

L

L

I

A

N

C

A

G

G

A

T

T

S

T

L

L

I

M

F

F

E

E

G

G

V

V

P

P

N
|
N

Y

W

P

P

D

T

H

P

G

A

R

R

F

M

W

C

D

Q

V

V

I

V

D

D

K

K

H

H

K

Q

V

V

N

N

L

I

F

L

Y

Y

T

T

A
|
A

P

P

T

T

A

A

I

I

R

R

A

A

L

L

M

M

R

A

E

E

G

G

D

D

S

K

F

A

V

I

T

E

S

G

R

T

N

D

L

R

S

S

L

L

R

R

V

I

L

L

G

G

T

S

V

V

G

G

E

E

P

P

I

I

N

N

P

P

E

E

A

A

W

W

R

E

W

W

Y

Y

Y

W

E

K

V

K

V

I

G

G

K

K

K

E

N

K

C

C

P

P

I

V

V

V

D

D

T

T

W

W

Q

Q

T

T

E

E

T

T

G

G

H

F

M

M

I

I

T

T

T

P

L

L

P

P

Y

G

A

A

H

I

D

E

M

L

K

K

P

A

G

G

S

S

A

A

G

T

K

R

P

P

F

F

G

G

I

V

Q

Q

P

P

L

A

L

L

V

V

D

D

N

N

D

E

G

G

N

H

T

P

L

Q

E

E

G

G

A

A

T

T

E

E

G

G

N

N

L

L

C

V

I

I

T

T

H

D

S

S

W

W

P

P

G

G

Q

Q

A

A

R

R

T

T

V

L

Y

F

G

G

D

D

H

H

D

E

R

R

F

F

E

E

Q

Q

A

T

Y

Y

F

F

S

S

T

T

Y

F

P

K

G

N

R

M

Y

Y

F

F

T

S

G

G

D

D

G

G

C

A

R

R

D

D

E

E

D

D

G

G

Y

Y

W

W

I

I

T

T

G

G

R

R

V

V

D
|
D

D

D

V

V

I

L

N

N

V

V

S
|
S

G
|
G

H

H

R
|
R

M

L

G

G

T

T

A

A

E

E

V

I

E

E

S

S

A

A

L

L

V

V

L

A

H

H

P

P

K

K

V

I

A

A

E

E

A

A

V

V

G

G

Y

I

P

P

H

H

D

A

I

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

C

A

Y

Y

V

V

T

T

L

L

N

N

A

H

G

G

E

E

G

P

S

S

D

P

E

E

L

L

Y

Y

S

A

E

E

L

V

R

R

Q

N

H

W

V

V

R
|
R

K

K

E

E

I

I

G

G

P

P

I

L

A

A

T

T

P

P

D

D

H

V

L

L

Q

H

F

W

T

T

D

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

E

A

N

G

D

D

Y

T

G

S

S

N

L

L

G

G

D

D

T

T

S

S

T

T

L

L

A

A

D

D

P

P

S

G

L

V

V

V

D

E

R

K

L

L

I

L

E

E

G

E

R

K

Q

Q

R194 (≠ K191) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 3-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 2-fold reduction for CoA.
T311 (= T305) binding CoA
N335 (= N329) binding CoA
A357 (= A351) mutation to V: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 3-fold reduction for CoA.
D517 (= D511) mutation to G: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 10-fold reduction for CoA.; mutation to P: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold reduction in the affinity for ATP and 4.5-fold reduction for CoA.
S523 (= S517) binding CoA
G524 (= G518) mutation to L: No acetyl-coenzyme A synthetase activity.; mutation to S: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. Almost the same affinity as the wild-type for ATP, but 9-fold reduction in the affinity for CoA.
R526 (= R520) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 4-fold reduction for CoA.
R584 (= R578) binding CoA; mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 7-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 8-fold reduction for CoA.
K609 (= K603) modified: N6-acetyllysine; by Pat; mutation to A: No acetyl-coenzyme A synthetase activity.

5jrhA Crystal structure of salmonella enterica acetyl-coa synthetase (acs) in complex with camp and coenzyme a (see paper)
64% identity, 97% coverage: 17:640/642 of query aligns to 16:639/640 of 5jrhA

query
sites
5jrhA

T

N

P

P

E

E

Q

Q

Y

Y

D

E

A

T

L

K

Y

Y

E

K

R

Q

S

S

V

I

Q

N

D

D

P

P

D

D

G

T

F

F

W

W

L

G

E

E

Q

Q

A

G

K

K

E

-

R

I

L

L

D

D

W

W

Y

I

T

T

A

P

P

Y

T

Q

K

K

G

V

A

K

E

N

W

T

S

S

Y

F

D

A

P

P

-

G

-

N

V

V

D

S

I

I

K

K

W

W

F

Y

S

E

D

D

G

G

T

T

L

L

N

N

I

L

C

A

Y

A

N

N

A

C

V

L

D

D

R

R

H

H

L

L

D

Q

T

E

H

N

G

G

D

D

Q

R

I

T

A

A

L

I

I

I

F
x
W

E

E

P

G

D

D

S

D

P

-

D

T

A

S

P

Q

G

S

R

K

S

H

L

I

T

S

Y

Y

R

R

Q

E

L

L

H

H

K

R

E

D

V

V

T

C

R

R

V

F

A

A

N

N

A

T

L

L

K

L

A

D

M

L

G

G

V

I

R

K

K

K

G

G

D

D

R

V

V

V

T

A

L

I

Y

Y

M

M

P

P

M

M

I

V

V

P

E
|
E

G

A

A

A

L

V

T

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

I

V

H

H

S

S

V

V

V

I

F
|
F

G
|
G

F

F

S

S

P

P

E

E

A

A

L

V

A

A

G
|
G

R

R

I

I

M

I

A

D

C

S

E

S

S

S

R

R

F

L

V

V

V

I

C

T

A

A

D

D

Q

E

G

G

L

V

R
|
R

G

A

S

G

K

R

T

S

V

I

P

P

L

L

K

K

A

K

N

N

V

V

D

D

A

D

A

A

L

L

A

K

M

N

Q

P

G

N

V

V

-

T

S

S

V

V

D

E

G

H

V

V

L

I

V

V

F

L

D

K

H

R

T

T

G

G

G

S

E

D

I

I

E

D

M

W

V

Q

D

E

G

G

R

R

D

D

H

L

W

W

F

W

A

R

D

D

F

L

-

I

-

E

-

K

A

A

S

S

D

P

D

E

E

H

C

Q

P

P

C

-

E

E

E

A

M

M

N

N

A

A

E

E

D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

T
x
K

P
|
P

K

K

G

G

V

V

L

L

H

H

T

T

G

G

Y

Y

A

L

L

V

W

Y

A

A

T

T

F

F

H

K

Y

Y

T

V

F

F

D

D

Y

Y

Q

H

P

P

G

G

D

D

I

I

F

Y

W

W

C

C

S

T

A

A

D

D

I

V

G

G

W

W

V

V

T

T

G

G

H

H

T

S

Y

Y

V

L

T

L

Y

Y

G

G

P

P

L

L

I

A

N

C

A

G

G

A

T

T

S

T

L

L

I

M

F

F

E

E

G

G

V

V

P

P

N

N

Y

W

P

P

D

T

H

P

G

A

R

R

F

M

W

C

D

Q

V

V

I

V

D

D

K

K

H

H

K

Q

V

V

N

N

L

I

F

L

Y

Y

T

T

A

A

P

P

T

T

A

A

I

I

R

R

A

A

L

L

M

M

R

A

E

E

G

G

D

D

S

K

F

A

V

I

T

E

S

G

R

T

N

D

L

R

S

S

L

L

R

R

V

I

L

L

G

G

T

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

R

E

W

W

Y

Y

Y

W

E

K

V

K

V

I

G

G

K

K

K

E

N

K

C

C

P

P

I

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

H

F

M

M

I

I

T

T

T

P

L

L

P

P

Y

G

A

A

H

I

D

E

M

L

K

K

P

A

G

G

S

S

A

A

G

T

K

R

P

P

F

F

G

G

I

V

Q

Q

P

P

L

A

L

L

V

V

D

D

N

N

D

E

G

G

N

H

T

P

L

Q

E

E

G

G

A

A

T

T

E

E

G

G

N

N

L

L

C

V

I

I

T

T

H

D

S

S

W

W

P

P

G

G

Q

Q

A

A

R

R

T

T

V

L

Y

F

G

G

D

D

H

H

D

E

R

R

F

F

E

E

Q

Q

A

T

Y

Y

F

F

S

S

T

T

Y

F

P

K

G

N

R

M

Y

Y

F

F

T

S

G

G

D
|
D

G

G

C

A

R

R

D

D

E

E

D

D

G

G

Y

Y

W

W

I
|
I

T

T

G

G

R

R

V

V

D

D

V

V

I

L

N
|
N

V

V

S
|
S

G

G

H

H

R
|
R

M

L

G

G

T

T

A

A

E

E

V

I

E

E

S

S

A

A

L

L

V
|
V

L

A

H
|
H

P

P

K

K

V
x
I

A

A

E

E

A

A

V

V

G

G

Y

I

P

P

H

H

D

A

I

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

C

A

Y

Y

V

V

T

T

L

L

N

N

A

H

G

G

E

E

G

P

S

S

D

P

E

E

L

L

Y

Y

S

A

E

E

L

V

R

R

Q

N

H

W

V

V

R
|
R

K

K

E

E

I

I

G

G

P
|
P

I

L

A

A

T

T

P

P

D

D

H

V

L

L

Q

H

F

W

T

T

D

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

E

A

N

G

D

D

Y

-

G

-

S

-

L

L

G

G

D

D

T

T

S

S

T

T

L

L

A

A

D

D

P

P

S

G

L

V

V

V

D

E

R

K

L

L

I

L

E

E

G

E

R

K

Q

Q

active site: T260 (= T258), T412 (= T410), E413 (= E411), N517 (= N515), R522 (= R520), K605 (= K603)
binding (r,r)-2,3-butanediol: W93 (≠ F93), E140 (= E141), G169 (= G170), K266 (≠ T264), P267 (= P265)
binding adenosine-3',5'-cyclic-monophosphate: G383 (= G381), E384 (= E382), P385 (= P383), T408 (= T406), W409 (= W407), W410 (= W408), Q411 (= Q409), T412 (= T410), D496 (= D494), I508 (= I506), N517 (= N515), R522 (= R520)
binding coenzyme a: F159 (= F160), G160 (= G161), G161 (= G162), R187 (= R188), S519 (= S517), R580 (= R578), P585 (= P583)
binding magnesium ion: V533 (= V531), H535 (= H533), I538 (≠ V536)

2p20A Acetyl-coa synthetase, r584a mutation (see paper)
63% identity, 97% coverage: 17:640/642 of query aligns to 16:640/641 of 2p20A

query
sites
2p20A

T

N

P

P

E

E

Q

Q

Y

Y

D

E

A

T

L

K

Y

Y

E

K

R

Q

S

S

V

I

Q

N

D

D

P

P

D

D

G

T

F

F

W

W

L

G

E

E

Q

Q

A

G

K

K

E

-

R

I

L

L

D

D

W

W

Y

I

T

T

A

P

P

Y

T

Q

K

K

G

V

A

K

E

N

W

T

S

S

Y

F

D

A

P

P

-

G

-

N

V

V

D

S

I

I

K

K

W

W

F

Y

S

E

D

D

G

G

T

T

L

L

N

N

I

L

C

A

Y

A

N

N

A

C

V

L

D

D

R

R

H

H

L

L

D

Q

T

E

H

N

G

G

D

D

Q

R

I

T

A

A

L

I

I

I

F

W

E

E

P

G

D

D

S

D

P

-

D

T

A

S

P

Q

G

S

R

K

S

H

L

I

T

S

Y

Y

R

R

Q

E

L

L

H

H

K

R

E

D

V

V

T

C

R

R

V

F

A

A

N

N

A

T

L

L

K

L

A

D

M

L

G

G

V

I

R

K

K

K

G

G

D

D

R

V

V

V

T

A

L

I

Y

Y

M

M

P

P

M

M

I

V

V

P

E

E

G

A

A

A

L

V

T

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

I

V

H

H

S

S

V

V

V

I

F

F

G

G

F

F

S

S

P

P

E

E

A

A

L

V

A

A

G

G

R

R

I

I

M

I

A

D

C

S

E

S

S

S

R

R

F

L

V

V

V

I

C

T

A

A

D

D

Q

E

G

G

L

V

R

R

G

A

S

G

K

R

T

S

V

I

P

P

L

L

K

K

A

K

N

N

V

V

D

D

A

D

A

A

L

L

A

K

M

N

Q

P

G

N

V

V

-

T

S

S

V

V

D

E

G

H

V

V

L

I

V

V

F

L

D

K

H

R

T

T

G

G

G

S

E

D

I

I

E

D

M

W

V

Q

D

E

G

G

R

R

D

D

H

L

W

W

F

W

A

R

D

D

F

L

-

I

-

E

-

K

A

A

S

S

D

P

D

E

E

H

C

Q

P

P

C

-

E

E

E

A

M

M

N

N

A

A

E

E

D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

T

K

P

P

K

K

G

G

V

V

L

L

H

H

T

T

G

G

Y

Y

A

L

L

V

W

Y

A

A

T

T

F

F

H

K

Y

Y

T

V

F

F

D

D

Y

Y

Q

H

P

P

G

G

D

D

I

I

F

Y

W

W

C

C

S

T

A

A

D

D

I

V

G

G

W

W

V

V

T

T

G

G

H

H

T

S

Y

Y

V

L

T

L

Y

Y

G

G

P

P

L

L

I

A

N

C

A

G

G

A

T

T

S

T

L

L

I

M

F

F

E

E

G

G

V

V

P

P

N

N

Y

W

P

P

D

T

H

P

G

A

R

R

F

M

W

C

D

Q

V

V

I

V

D

D

K

K

H

H

K

Q

V

V

N

N

L

I

F

L

Y

Y

T

T

A

A

P

P

T

T

A

A

I

I

R

R

A

A

L

L

M

M

R

A

E

E

G

G

D

D

S

K

F

A

V

I

T

E

S

G

R

T

N

D

L

R

S

S

L

L

R

R

V

I

L

L

G

G

T

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

R

E

W

W

Y

Y

Y

W

E

K

V

K

V

I

G

G

K

K

K

E

N

K

C

C

P

P

I

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

H

F

M

M

I

I

T

T

T

P

L

L

P

P

Y

G

A

A

H

I

D

E

M

L

K

K

P

A

G

G

S

S

A

A

G

T

K

R

P

P

F

F

G

G

I

V

Q

Q

P

P

L

A

L

L

V

V

D

D

N

N

D

E

G

G

N

H

T

P

L

Q

E

E

G

G

A

A

T

T

E

E

G

G

N

N

L

L

C

V

I

I

T

T

H

D

S

S

W

W

P

P

G

G

Q

Q

A

A

R

R

T

T

V

L

Y

F

G

G

D

D

H

H

D

E

R

R

F

F

E

E

Q

Q

A

T

Y

Y

F

F

S

S

T

T

Y

F

P

K

G

N

R

M

Y

Y

F

F

T

S

G

G

D
|
D

G

G

C

A

R

R

D

D

E

E

D

D

G

G

Y

Y

W

W

I
|
I

T

T

G

G

R
|
R

V

V

D

D

V

V

I

L

N
|
N

V

V

S

S

G

G

H

H

R
|
R

M

L

G

G

T

T

A

A

E

E

V

I

E

E

S

S

A

A

L

L

V

V

L

A

H

H

P

P

K

K

V

I

A

A

E

E

A

A

V

V

G

G

Y

I

P

P

H

H

D

A

I

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

C

A

Y

Y

V

V

T

T

L

L

N

N

A

H

G

G

E

E

G

P

S

S

D

P

E

E

L

L

Y

Y

S

A

E

E

L

V

R

R

Q

N

H

W

V

V

R

A

K

K

E

E

I

I

G

G

P

P

I

L

A

A

T

T

P

P

D

D

H

V

L

L

Q

H

F

W

T

T

D

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

E

A

N

G

D

D

Y

T

G

S

S

N

L

-

G

-

D

D

T

T

S

S

T

T

L

L

A

A

D

D

P

P

S

G

L

V

V

V

D

E

R

K

L

L

I

L

E

E

G

E

R

K

Q

Q

active site: T260 (= T258), T412 (= T410), E413 (= E411), N517 (= N515), R522 (= R520), K605 (= K603)
binding adenosine-5'-monophosphate-propyl ester: G383 (= G381), E384 (= E382), P385 (= P383), T408 (= T406), W409 (= W407), W410 (= W408), Q411 (= Q409), T412 (= T410), D496 (= D494), I508 (= I506), R511 (= R509), R522 (= R520)

2p2fA Acetyl-coa synthetase, wild-type with acetate, amp, and coa bound (see paper)
63% identity, 97% coverage: 17:640/642 of query aligns to 15:636/637 of 2p2fA

query
sites
2p2fA

T

N

P

P

E

E

Q

Q

Y

Y

D

E

A

T

L

K

Y

Y

E

K

R

Q

S

S

V

I

Q

N

D

D

P

P

D

D

G

T

F

F

W

W

L

G

E

E

Q

Q

A

G

K

K

E

-

R

I

L

L

D

D

W

W

Y

I

T

T

A

P

P

Y

T

Q

K

K

G

V

A

K

E

N

W

T

S

S

Y

F

D

A

P

P

-

G

-

N

V

V

D

S

I

I

K

K

W

W

F

Y

S

E

D

D

G

G

T

T

L

L

N

N

I

L

C

A

Y

A

N

N

A

C

V

L

D

D

R

R

H

H

L

L

D

Q

T

E

H

N

G

G

D

D

Q

R

I

T

A

A

L

I

I

I

F

W

E

E

P

G

D

D

S

D

P

-

D

T

A

S

P

Q

G

S

R

K

S

H

L

I

T

S

Y

Y

R

R

Q

E

L

L

H

H

K

R

E

D

V

V

T

C

R

R

V

F

A

A

N

N

A

T

L

L

K

L

A

D

M

L

G

G

V

I

R

K

K

K

G

G

D

D

R

V

V

V

T

A

L

I

Y

Y

M

M

P

P

M

M

I

V

V

P

E

E

G

A

A

A

L

V

T

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

I

V

H

H

S

S

V

V

V

I

F
|
F

G

G

F

F

S

S

P

P

E

E

A

A

L

V

A

A

G

G

R

R

I

I

M

I

A

D

C

S

E

S

S

S

R

R

F

L

V

V

V

I

C

T

A

A

D

D

Q

E

G

G

L

V

R
|
R

G

A

S

G

K

R

T

S

V

I

P

P

L

L

K

K

A

K

N

N

V

V

D

D

A

D

A

A

L

L

A

K

M

N

Q

P

G

N

V

V

-

T

S

S

V

V

D

E

G

H

V

V

L

I

V

V

F

L

D

K

H

R

T

T

G

G

G

S

E

D

I

I

E

D

M

W

V

Q

D

E

G

G

R

R

D

D

H

L

W

W

F

W

A

R

D

D

F

L

-

I

-

E

-

K

A

A

S

S

D

P

D

E

E

H

C

Q

P

P

C

-

E

E

E

A

M

M

N

N

A

A

E

E

D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

T

K

P

P

K

K

G

G

V

V

L

L

H

H

T

T

G

G

Y

Y

A

L

L

V

W

Y

A

A

T

T

F

F

H

K

Y

Y

T

V

F

F

D

D

Y

Y

Q

H

P

P

G

G

D

D

I

I

F

Y

W

W

C

C

S

T

A

A

D

D

I

V

G

G

W
|
W

V

V

T
|
T

G

G

H

H

T

S

Y

Y

V

L

T

L

Y

Y

G

G

P

P

L

L

I

A

N

C

A

G

G

A

T

T

S

T

L

L

I

M

F

F

E

E

G

G

V

V

P
|
P

N

N

Y

W

P

P

D

T

H

P

G

A

R

R

F

M

W

C

D

Q

V

V

I

V

D

D

K

K

H

H

K

Q

V

V

N

N

L

I

F

L

Y

Y

T

T

A
|
A

P

P

T

T

A
|
A

I

I

R

R

A

A

L

L

M

M

R

A

E

E

G

G

D

D

S

K

F

A

V

I

T

E

S

G

R

T

N

D

L

R

S

S

L

L

R

R

V

I

L

L

G

G

T

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

R

E

W

W

Y

Y

Y

W

E

K

V

K

V

I

G

G

K

K

K

E

N

K

C

C

P

P

I

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

H

F

M

M

I

I

T

T

T

P

L

L

P

P

Y

G

A

A

H

I

D

E

M

L

K

K

P

A

G

G

S

S

A

A

G

T

K

R

P

P

F

F

G

G

I

V

Q

Q

P

P

L

A

L

L

V

V

D

D

N

N

D

E

G

G

N

H

T

P

L

Q

E

E

G

G

A

A

T

T

E

E

G

G

N

N

L

L

C

V

I

I

T

T

H

D

S

S

W

W

P

P

G

G

Q

Q

A

A

R

R

T

T

V

L

Y

F

G

G

D

D

H

H

D

E

R

R

F

F

E

E

Q

Q

A

T

Y

Y

F

F

S

S

T

T

Y

F

P

K

G

N

R

M

Y

Y

F

F

T

S

G

G

D
|
D

G

G

C

A

R

R

D

D

E

E

D

D

G

G

Y

Y

W

W

I
|
I

T

T

G

G

R
|
R

V

V

D

D

V

V

I

L

N
|
N

V

V

S
|
S

G

G

H

H

R
|
R

M

L

G

G

T

T

A

A

E

E

V

I

E

E

S

S

A

A

L

L

V

V

L

A

H

H

P

P

K

K

V

I

A

A

E

E

A

A

V

V

G

G

Y

I

P

P

H

H

D

A

I

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

C

A

Y

Y

V

V

T

T

L

L

N

N

A

H

G

G

E

E

G

P

S

S

D

P

E

E

L

L

Y

Y

S

A

E

E

L

V

R

R

Q

N

H

W

V

V

R
|
R

K

K

E

E

I

I

G

G

P
|
P

I

L

A

A

T

T

P

P

D

D

H

V

L

L

Q

H

F

W

T

T

D

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

E

A

N

G

D

D

Y

-

G

-

S

-

L

-

G

-

D

T

T

S

S

S

T

T

L

L

A

A

D

D

P

P

S

G

L

V

V

V

D

E

R

K

L

L

I

L

E

E

G

E

R

K

Q

Q

active site: T259 (= T258), T411 (= T410), E412 (= E411), N516 (= N515), R521 (= R520), K604 (= K603)
binding adenosine monophosphate: G382 (= G381), E383 (= E382), P384 (= P383), T407 (= T406), W408 (= W407), W409 (= W408), Q410 (= Q409), T411 (= T410), D495 (= D494), I507 (= I506), R510 (= R509), N516 (= N515), R521 (= R520)
binding coenzyme a: F158 (= F160), R186 (= R188), W304 (= W303), T306 (= T305), P329 (= P328), A352 (= A351), A355 (= A354), S518 (= S517), R579 (= R578), P584 (= P583)

1pg3A Acetyl coa synthetase, acetylated on lys609 (see paper)
63% identity, 97% coverage: 17:640/642 of query aligns to 16:633/634 of 1pg3A

query
sites
1pg3A

T

N

P

P

E

E

Q

Q

Y

Y

D

E

A

T

L

K

Y

Y

E

K

R

Q

S

S

V

I

Q

N

D

D

P

P

D

D

G

T

F

F

W

W

L

G

E

E

Q

Q

A

G

K

K

E

-

R

I

L

L

D

D

W

W

Y

I

T

T

A

P

P

Y

T

Q

K

K

G

V

A

K

E

N

W

T

S

S

Y

F

D

A

P

P

-

G

-

N

V

V

D

S

I

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active site: T260 (= T258), T412 (= T410), E413 (= E411), N517 (= N515), R522 (= R520), K605 (= K603)
binding coenzyme a: F159 (= F160), G160 (= G161), R187 (= R188), R190 (≠ K191), A301 (= A299), T307 (= T305), P330 (= P328), A356 (= A354), S519 (= S517), R580 (= R578), P585 (= P583)
binding magnesium ion: V533 (= V531), H535 (= H533), I538 (≠ V536)
binding adenosine-5'-monophosphate-propyl ester: G383 (= G381), E384 (= E382), P385 (= P383), T408 (= T406), W409 (= W407), W410 (= W408), Q411 (= Q409), T412 (= T410), D496 (= D494), R511 (= R509), R522 (= R520)

Q9QXG4 Acetyl-coenzyme A synthetase, cytoplasmic; Acetate--CoA ligase; Acetyl-CoA synthetase; ACS; AceCS; Acetyl-CoA synthetase 1; AceCS1; Acyl-CoA synthetase short-chain family member 2; Acyl-activating enzyme; Propionate--CoA ligase; EC 6.2.1.1; EC 6.2.1.17 from Mus musculus (Mouse) (see paper)
53% identity, 97% coverage: 19:639/642 of query aligns to 45:696/701 of Q9QXG4

query
sites
Q9QXG4

E

Q

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