SitesBLAST – Find functional sites

 

SitesBLAST

Comparing WP_047007751.1 NCBI__GCF_001010925.1:WP_047007751.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

1sz2B Crystal structure of e. Coli glucokinase in complex with glucose (see paper)
33% identity, 98% coverage: 7:322/324 of query aligns to 8:317/320 of 1sz2B

query
sites
1sz2B
D
 
D
I
 
V
G
 
G
G
 
G
T
 
T
H
 
N
A
 
A
R
 
R
F
 
L
A
 
A
I
 
L
A
 
C
T
 
-
H
 
-
A
 
-
D
 
D
D
 
I
G
 
A
S
 
S
I
 
G
T
 
E
M
 
I
D
 
S
E
 
Q
P
 
A
V
 
K
T
 
T
L
 
Y
H
 
S
T
 
G
D
 
L
D
 
D
H
 
Y
A
 
P
S
 
S
F
 
L
Q
 
E
T
 
A
A
 
V
W
 
I
E
 
R
D
 
V
Y
 
Y
R
 
L
A
 
E
Q
 
E
K
 
H
G
 
K
G
 
V
D
 
E
I
 
V
P
 
K
S
 
D
R
 
G
V
 
-
A
 
C
M
 
I
A
 
A
I
 
I
A
 
A
G
x
C
P
|
P
V
 
I
G
 
T
G
 
G
E
 
D
V
 
W
I
 
V
R
 
A
F
 
M
T
 
T
N
 
N
N
 
H
P
 
T
W
 
W
I
 
A
I
 
F
R
 
S
P
 
I
A
 
A
L
 
E
V
 
M
K
 
K
E
 
K
K
 
N
L
 
L
G
 
G
V
 
F
D
 
S
S
 
H
F
 
L
K
 
E
I
 
I
V
 
I
N
|
N
D
|
D
F
 
F
E
 
T
A
 
A
V
 
V
A
 
S
H
 
M
A
 
A
V
 
I
A
 
P
R
 
M
A
 
L
G
 
K
E
 
K
D
 
E
E
 
H
Y
 
L
L
 
I
H
 
Q
L
 
F
T
 
G
G
 
G
P
 
A
E
 
E
G
 
-
P
 
-
L
 
-
P
 
P
P
 
V
T
 
E
G
 
G
R
 
K
-
 
P
L
 
I
T
 
A
V
 
V
M
 
Y
G
 
G
P
 
A
G
 
G
T
 
T
G
 
G
L
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L
G
|
G
V
 
V
A
 
A
H
 
H
L
 
L
Y
 
V
R
 
H
E
 
-
A
 
V
D
 
D
G
 
K
T
 
R
Y
 
W
R
 
V
V
 
S
S
 
L
A
 
P
T
 
G
E
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E
G
 
G
G
 
G
H
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H
I
 
V
D
 
D
F
 
F
A
 
A
P
 
P
L
 
N
D
 
S
S
 
E
I
 
E
E
 
E
D
 
A
A
 
I
I
 
I
L
 
L
A
 
E
R
 
I
L
 
L
R
 
R
K
 
A
R
 
E
H
 
I
T
 
G
R
 
H
V
 
V
S
 
S
V
 
A
E
|
E
R
 
R
V
 
V
V
 
L
A
 
S
G
 
G
P
 
P
A
 
G
I
 
L
S
 
V
D
 
N
I
 
L
Y
 
Y
H
 
R
T
 
A
L
 
I
A
 
V
S
 
K
L
 
A
E
 
D
R
 
N
K
 
R
S
 
L
V
 
P
P
 
E
E
 
N
L
 
L
D
 
K
D
 
P
V
 
K
E
 
D
I
 
I
W
 
T
T
 
E
R
 
R
G
 
A
M
 
L
N
 
A
G
 
D
E
 
S
D
 
C
S
 
T
L
 
D
A
 
C
A
 
R
A
 
R
A
 
A
V
 
L
D
 
S
R
 
L
F
 
F
C
 
C
L
 
V
S
 
I
L
 
M
G
 
G
S
 
R
V
 
F
A
 
G
G
 
G
D
 
N
M
 
L
A
 
A
L
 
L
A
 
N
H
 
L
G
 
G
A
 
T
-
 
F
K
 
G
A
 
G
V
 
V
V
 
F
I
 
I
A
 
A
G
 
G
G
 
G
L
 
I
G
 
V
Y
 
P
R
 
R
L
 
F
R
 
L
D
 
E
H
 
F
L
 
F
P
 
K
K
 
A
S
 
S
G
 
G
F
 
F
A
 
R
E
 
A
R
 
A
F
 
F
R
 
E
A
 
D
K
 
K
G
 
G
R
 
R
F
 
F
A
 
K
G
 
E
L
 
Y
M
 
V
A
 
H
G
 
D
L
 
I
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P
V
 
V
K
 
Y
L
 
L
I
 
I
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V
H
 
H
P
 
D
Q
 
N
P
 
P
G
 
G
L
 
L
F
 
L
G
 
G
A
 
S
A
 
G
A
 
A
A
 
H
Y
 
L
C
 
R
Q
 
Q
K
 
T
F
 
L
G
 
G

6da0A Crystal structure of glucokinase (nfhk) from naegleria fowleri (see paper)
26% identity, 52% coverage: 3:169/324 of query aligns to 28:213/378 of 6da0A

query
sites
6da0A
L
 
I
V
 
V
T
 
G
V
 
V
D
 
D
I
 
V
G
|
G
G
 
A
T
|
T
H
x
N
A
 
T
R
 
R
-
 
I
-
 
A
-
 
I
-
 
Q
F
 
F
A
 
I
I
 
I
A
 
N
T
 
E
H
 
D
A
 
Q
D
 
D
D
 
D
G
 
E
S
 
V
I
 
F
T
 
M
M
 
T
D
 
K
E
 
F
P
 
P
V
 
C
T
 
-
L
 
-
H
 
N
T
 
T
D
 
S
D
 
T
H
 
H
-
 
L
A
 
A
S
 
N
F
 
Y
Q
 
L
T
 
A
A
 
A
W
 
Y
E
 
G
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K
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A
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M
A
 
V
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K
K
 
A
G
 
V
G
 
G
D
 
K
I
 
G
P
 
S
S
 
A
R
 
A
V
 
G
A
 
S
M
 
I
A
 
A
I
 
L
A
 
A
G
|
G
P
 
P
V
 
V
G
 
T
G
 
G
E
 
D
V
 
K
I
 
V
R
 
R
F
 
I
T
|
T
N
 
N
-
 
Y
-
 
K
-
 
E
-
 
H
N
 
D
P
 
Q
W
 
E
I
 
F
I
 
F
R
 
Y
P
 
S
A
 
Q
L
 
L
V
 
P
K
 
D
E
 
T
K
 
L
L
 
F
G
 
P
V
 
A
D
 
S
S
 
K
F
 
N
K
 
T
I
 
F
V
 
L
N
|
N
D
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D
F
 
L
E
 
E
A
 
A
V
 
S
A
 
C
H
 
Y
A
 
G
V
 
I
A
 
I
R
 
N
A
 
V
G
 
G
E
 
T
D
 
N
E
 
N
Y
 
R
L
 
L
H
 
H
-
 
E
-
 
F
-
 
F
-
 
C
-
 
P
-
 
I
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D
-
 
A
-
 
L
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N
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N
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L
 
A
T
 
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G
 
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Q
E
 
T
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V
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R
L
 
L
P
 
S
P
 
D
T
 
T
G
 
S
R
 
E
L
 
Y
T
 
A
V
 
V
M
 
L
G
 
A
P
 
M
G
|
G
T
|
T
G
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G
L
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L
G
|
G
V
 
T
A
 
G
H
 
L
L
 
I
Y
 
V
R
 
G
E
 
S
A
 
A
D
 
G
G
 
G
T
 
K
Y
 
F
R
 
N
V
 
V
S
 
I
A
 
P
T
 
L
E
|
E
G
 
A
G
 
G
H
|
H
I
 
V
D
 
H
F
 
I
A
 
A

Sites not aligning to the query:

Query Sequence

>WP_047007751.1 NCBI__GCF_001010925.1:WP_047007751.1
MQLVTVDIGGTHARFAIATHADDGSITMDEPVTLHTDDHASFQTAWEDYRAQKGGDIPSR
VAMAIAGPVGGEVIRFTNNPWIIRPALVKEKLGVDSFKIVNDFEAVAHAVARAGEDEYLH
LTGPEGPLPPTGRLTVMGPGTGLGVAHLYREADGTYRVSATEGGHIDFAPLDSIEDAILA
RLRKRHTRVSVERVVAGPAISDIYHTLASLERKSVPELDDVEIWTRGMNGEDSLAAAAVD
RFCLSLGSVAGDMALAHGAKAVVIAGGLGYRLRDHLPKSGFAERFRAKGRFAGLMAGLPV
KLITHPQPGLFGAAAAYCQKFGDD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory