SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_047093014.1 NCBI__GCF_001013305.1:WP_047093014.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

1sz2B Crystal structure of e. Coli glucokinase in complex with glucose (see paper)
34% identity, 97% coverage: 8:323/326 of query aligns to 8:317/320 of 1sz2B

query
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1sz2B

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binding beta-D-glucopyranose: C64 (≠ G68), P65 (= P69), N98 (= N102), D99 (= D103), L138 (= L144), G139 (= G145), E156 (= E163), H159 (= H166), E186 (= E193)

6da0A Crystal structure of glucokinase (nfhk) from naegleria fowleri (see paper)
26% identity, 51% coverage: 4:170/326 of query aligns to 28:213/378 of 6da0A

query
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binding phosphoaminophosphonic acid-adenylate ester: G34 (= G10), T36 (= T12), N37 (≠ H13), G184 (= G141), T185 (= T142)
binding beta-D-glucopyranose: G95 (= G68), T105 (= T78), N133 (= N102), D134 (= D103), G186 (= G143), L187 (= L144), G188 (= G145), E206 (= E163), H209 (= H166)

Sites not aligning to the query:

8dtcA Crystal structure of glucokinase with bound glucose from acanthamoeba castellanii
25% identity, 98% coverage: 1:320/326 of query aligns to 23:356/374 of 8dtcA

query
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8dtcA

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binding beta-D-glucopyranose: G32 (= G10), G33 (= G11), T34 (= T12), N35 (≠ H13), R37 (= R15), G91 (= G68), T103 (= T78), N133 (= N102), D134 (= D103), L181 (= L144), G182 (= G145), E204 (= E163), H207 (= H166), E243 (= E193)

Query Sequence

>WP_047093014.1 NCBI__GCF_001013305.1:WP_047093014.1
MTEIVTVDIGGTHARFALASCTADGGISMDDPVTLHTQDHVSFQTAWEDYRAQKGGTLPR
HVAMAIAGPVGGDVIRFTNNPWIIRPALVSEKLGVDDYVLVNDFEAVAHAVARAQDDEFL
HLTGPEGALPATGRLTVLGPGTGLGVAHLYREPDGTYRVSATEGGHIDFAPLDQIEDAIL
ARLRKRHNRVSVERVVAGPAISDIYHTLAALENKSVPELADIEIWTKGMSGEDSLAAAAV
DRFCLSLGSVAGDMALAHGAKAVVIAGGLGYRLRNHLPNSGFAERFRAKGRFESLMAGLP
VKLITHPQPGLFGAAAAYFQKFGEQK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory