SitesBLAST
Comparing WP_047094365.1 NCBI__GCF_001013305.1:WP_047094365.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
31% identity, 97% coverage: 8:252/252 of query aligns to 11:258/259 of 5zaiC
- active site: A65 (= A64), F70 (≠ M69), S82 (≠ A81), R86 (≠ N87), G110 (= G111), E113 (≠ G114), P132 (≠ T133), E133 (≠ P134), I138 (= I139), P140 (≠ Y141), G141 (≠ P142), A226 (≠ D219), F236 (= F230)
- binding coenzyme a: K24 (= K21), L25 (≠ R22), A63 (= A62), G64 (= G63), A65 (= A64), D66 (= D65), I67 (= I66), P132 (≠ T133), R166 (≠ L167), F248 (= F242), K251 (= K245)
5jbxB Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
31% identity, 100% coverage: 2:252/252 of query aligns to 6:260/261 of 5jbxB
- active site: A67 (= A64), R72 (≠ M69), L84 (≠ Q83), R88 (≠ N87), G112 (= G111), E115 (≠ G114), T134 (= T133), E135 (≠ P134), I140 (= I139), P142 (≠ Y141), G143 (≠ P142), A228 (= A224), L238 (≠ F230)
- binding coenzyme a: S24 (≠ D20), R25 (≠ K21), R26 (= R22), A28 (= A24), A65 (= A62), D68 (= D65), L69 (≠ I66), K70 (= K67), L110 (≠ V109), G111 (= G110), T134 (= T133), E135 (≠ P134), L138 (= L137), R168 (≠ L167)
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
31% identity, 98% coverage: 5:251/252 of query aligns to 7:254/256 of 3h81A
- active site: A64 (= A64), M69 (= M69), T79 (≠ Q83), F83 (≠ N87), G107 (= G111), E110 (≠ G114), P129 (≠ T133), E130 (≠ P134), V135 (≠ I139), P137 (≠ Y141), G138 (≠ P142), L223 (≠ D220), F233 (= F230)
- binding calcium ion: F233 (= F230), Q238 (≠ F235)
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
31% identity, 99% coverage: 2:251/252 of query aligns to 5:255/255 of 3q0jC
- active site: A65 (= A64), M70 (= M69), T80 (≠ Q83), F84 (≠ N87), G108 (= G111), E111 (≠ G114), P130 (≠ T133), E131 (≠ P134), V136 (≠ I139), P138 (≠ Y141), G139 (≠ P142), L224 (≠ D220), F234 (= F230)
- binding acetoacetyl-coenzyme a: Q23 (≠ D20), A24 (≠ K21), L25 (≠ R22), A27 (= A24), A63 (= A62), G64 (= G63), A65 (= A64), D66 (= D65), I67 (= I66), K68 (= K67), M70 (= M69), F84 (≠ N87), G107 (= G110), G108 (= G111), E111 (≠ G114), P130 (≠ T133), E131 (≠ P134), P138 (≠ Y141), G139 (≠ P142), M140 (≠ L143)
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
31% identity, 99% coverage: 2:251/252 of query aligns to 5:255/255 of 3q0gC
- active site: A65 (= A64), M70 (= M69), T80 (≠ Q83), F84 (≠ N87), G108 (= G111), E111 (≠ G114), P130 (≠ T133), E131 (≠ P134), V136 (≠ I139), P138 (≠ Y141), G139 (≠ P142), L224 (≠ D220), F234 (= F230)
- binding coenzyme a: L25 (≠ R22), A63 (= A62), I67 (= I66), K68 (= K67), Y104 (≠ D107), P130 (≠ T133), E131 (≠ P134), L134 (= L137)
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
30% identity, 99% coverage: 2:251/252 of query aligns to 4:250/250 of 3q0gD
- active site: A64 (= A64), M69 (= M69), T75 (≠ D75), F79 (≠ L79), G103 (= G111), E106 (≠ G114), P125 (≠ T133), E126 (≠ P134), V131 (≠ I139), P133 (≠ Y141), G134 (≠ P142), L219 (≠ D220), F229 (= F230)
- binding Butyryl Coenzyme A: F225 (= F226), F241 (= F242)
Q8GYN9 1,4-dihydroxy-2-naphthoyl-CoA synthase, peroxisomal; DHNS; Enoyl-CoA hydratase/isomerase D; ECHID; Naphthoate synthase; EC 4.1.3.36 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
30% identity, 97% coverage: 7:251/252 of query aligns to 83:331/337 of Q8GYN9
Sites not aligning to the query:
- 20 H→V: Loss of peroxisomal targeting.
Q13825 Methylglutaconyl-CoA hydratase, mitochondrial; 3-MG-CoA hydratase; AU-specific RNA-binding enoyl-CoA hydratase; AU-binding protein/enoyl-CoA hydratase; Itaconyl-CoA hydratase; EC 4.2.1.18; EC 4.2.1.56 from Homo sapiens (Human) (see 4 papers)
30% identity, 94% coverage: 16:252/252 of query aligns to 94:338/339 of Q13825
- K105 (≠ M27) mutation to N: Abolishes RNA-binding; when associated with E-109 and Q-113.
- 105:119 (vs. 27:41, 0% identical) RNA-binding
- K109 (≠ E31) mutation to E: Abolishes RNA-binding; when associated with N-105 and Q-113.
- K113 (≠ G35) mutation to Q: Abolishes RNA-binding; when associated with N-105 and E-109.
- A240 (≠ G165) to V: in MGCA1; decreased methylglutaconyl-CoA hydratase activity; dbSNP:rs769894315
Sites not aligning to the query:
- 1:67 modified: transit peptide, Mitochondrion
O53561 Enoyl-CoA hydratase EchA19; EC 4.2.1.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
31% identity, 95% coverage: 13:251/252 of query aligns to 17:264/266 of O53561
- K135 (≠ R129) modified: N6-succinyllysine; mutation to E: Nearly wild-type levels of succinylation in vitro, reduces specific activity 8-fold.
- 135:142 (vs. 129:136, 50% identical) mutation to EFGISEAE: Very low levels of succinylation in vitro, reduces specific activity 15-fold.
- K142 (= K136) modified: N6-succinyllysine; mutation to E: About 50% succinylation in vitro, reduces specific activity 7-fold.
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
28% identity, 96% coverage: 8:250/252 of query aligns to 14:255/258 of 1mj3A
- active site: A68 (= A64), M73 (= M69), S83 (≠ A84), L85 (≠ I86), G109 (= G111), E112 (≠ G114), P131 (≠ T133), E132 (≠ P134), T137 (≠ I139), P139 (≠ Y141), G140 (≠ P142), K225 (vs. gap), F235 (= F230)
- binding hexanoyl-coenzyme a: K26 (≠ D20), A27 (≠ K21), L28 (≠ R22), A30 (= A24), A66 (= A62), G67 (= G63), A68 (= A64), D69 (= D65), I70 (= I66), G109 (= G111), P131 (≠ T133), E132 (≠ P134), L135 (= L137), G140 (≠ P142)
4i52A Scmenb im complex with 1-hydroxy-2-naphthoyl-coa (see paper)
30% identity, 96% coverage: 9:251/252 of query aligns to 18:269/275 of 4i52A
- active site: G77 (≠ A64), R82 (≠ K73), Y87 (≠ W78), R95 (≠ N87), L99 (≠ H91), G123 (= G111), V126 (≠ G114), G146 (≠ P134), S151 (≠ I139), D153 (≠ Y141), G154 (≠ P142), A240 (≠ T222), Y248 (≠ F230)
- binding 1-hydroxy-2-naphthoyl-CoA: H29 (≠ D20), K30 (= K21), R31 (= R22), A33 (= A24), S75 (≠ A62), G76 (= G63), G77 (≠ A64), D78 (= D65), Q79 (≠ I66), L96 (≠ R88), V98 (≠ Q90), Y119 (≠ D107), I121 (≠ V109), G123 (= G111), T145 (= T133), V149 (≠ L137), S151 (≠ I139), F152 (≠ V140)
4i4zA Synechocystis sp. Pcc 6803 1,4-dihydroxy-2-naphthoyl-coenzyme a synthase (menb) in complex with salicylyl-coa (see paper)
30% identity, 96% coverage: 9:251/252 of query aligns to 18:269/275 of 4i4zA
- active site: G77 (≠ A64), R82 (≠ K73), Y87 (≠ W78), R95 (≠ N87), L99 (≠ H91), G123 (= G111), V126 (≠ G114), G146 (≠ P134), S151 (≠ I139), D153 (≠ Y141), G154 (≠ P142), A240 (≠ T222), Y248 (≠ F230)
- binding Salicylyl CoA: H29 (≠ D20), K30 (= K21), R31 (= R22), S75 (≠ A62), G76 (= G63), G77 (≠ A64), D78 (= D65), Q79 (≠ I66), Y87 (≠ W78), V98 (≠ Q90), G123 (= G111), T145 (= T133), V149 (≠ L137), S151 (≠ I139), F260 (= F242), K263 (= K245)
- binding bicarbonate ion: G122 (= G110), Q144 (≠ I132), T145 (= T133), G146 (≠ P134), W174 (≠ L162)
6p5uE Structure of an enoyl-coa hydratase/aldolase isolated from a lignin- degrading consortium (see paper)
31% identity, 83% coverage: 2:211/252 of query aligns to 7:223/246 of 6p5uE
- active site: M67 (≠ A64), Y72 (≠ M69), D77 (= D74), R89 (vs. gap), A93 (≠ N87), G117 (= G111), T120 (≠ G114), E140 (≠ P134), I145 (= I139), P147 (≠ Y141), A148 (≠ P142)
- binding coenzyme a: D25 (= D20), K26 (= K21), R27 (= R22), A29 (= A24), A65 (= A62), M67 (≠ A64), D68 (= D65), L69 (≠ I66), W113 (≠ D107), F115 (≠ V109), S139 (≠ T133), W143 (≠ L137)
Sites not aligning to the query:
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
28% identity, 96% coverage: 8:250/252 of query aligns to 13:251/254 of 2dubA
- active site: A67 (= A64), M72 (= M69), S82 (≠ Q90), G105 (= G111), E108 (≠ G114), P127 (≠ T133), E128 (≠ P134), T133 (≠ I139), P135 (≠ Y141), G136 (≠ P142), K221 (vs. gap), F231 (= F230)
- binding octanoyl-coenzyme a: K25 (≠ D20), A26 (≠ K21), L27 (≠ R22), A29 (= A24), A65 (= A62), A67 (= A64), D68 (= D65), I69 (= I66), K70 (= K67), G105 (= G111), E108 (≠ G114), P127 (≠ T133), E128 (≠ P134), G136 (≠ P142), A137 (≠ L143)
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
28% identity, 96% coverage: 8:250/252 of query aligns to 14:257/260 of 1dubA
- active site: A68 (= A64), M73 (= M69), S83 (≠ N82), L87 (≠ I86), G111 (= G111), E114 (≠ G114), P133 (≠ T133), E134 (≠ P134), T139 (≠ I139), P141 (≠ Y141), G142 (≠ P142), K227 (vs. gap), F237 (= F230)
- binding acetoacetyl-coenzyme a: K26 (≠ D20), A27 (≠ K21), L28 (≠ R22), A30 (= A24), A66 (= A62), A68 (= A64), D69 (= D65), I70 (= I66), Y107 (≠ D107), G110 (= G110), G111 (= G111), E114 (≠ G114), P133 (≠ T133), E134 (≠ P134), L137 (= L137), G142 (≠ P142), F233 (= F226), F249 (= F242)
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
28% identity, 96% coverage: 8:250/252 of query aligns to 12:255/258 of 1ey3A
- active site: A66 (= A64), M71 (= M69), S81 (≠ N82), L85 (≠ I86), G109 (= G111), E112 (≠ G114), P131 (≠ T133), E132 (≠ P134), T137 (≠ I139), P139 (≠ Y141), G140 (≠ P142), K225 (vs. gap), F235 (= F230)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (≠ D20), L26 (≠ R22), A28 (= A24), A64 (= A62), G65 (= G63), A66 (= A64), D67 (= D65), I68 (= I66), L85 (≠ I86), W88 (≠ V89), G109 (= G111), P131 (≠ T133), L135 (= L137), G140 (≠ P142)
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
28% identity, 96% coverage: 8:250/252 of query aligns to 44:287/290 of P14604