SitesBLAST
Comparing WP_047094432.1 NCBI__GCF_001013305.1:WP_047094432.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3fpcA Chimera of alcohol dehydrogenase by exchange of the cofactor binding domain res 153-294 of t. Brockii adh by e. Histolytica adh (see paper)
37% identity, 99% coverage: 1:349/354 of query aligns to 1:346/352 of 3fpcA
- active site: C37 (= C37), T38 (≠ G38), S39 (≠ T39), H42 (= H42), H59 (= H58), E60 (= E59), D89 (≠ S88), T92 (≠ S91), V95 (≠ S94), S103 (≠ C102), D150 (= D156), T154 (= T160), K346 (= K349)
- binding zinc ion: C37 (= C37), H59 (= H58), D150 (= D156)
P35630 NADP-dependent isopropanol dehydrogenase; EhADH1; EC 1.1.1.80 from Entamoeba histolytica (strain ATCC 30459 / HM-1:IMSS / ABRM) (see paper)
37% identity, 99% coverage: 1:349/354 of query aligns to 7:352/366 of P35630
- C43 (= C37) binding Zn(2+)
- H65 (= H58) binding Zn(2+)
- E66 (= E59) binding Zn(2+)
- D156 (= D156) binding Zn(2+)
1y9aA Alcohol dehydrogenase from entamoeba histolotica in complex with cacodylate (see paper)
37% identity, 99% coverage: 1:349/354 of query aligns to 1:346/360 of 1y9aA
- active site: C37 (= C37), T38 (≠ G38), S39 (≠ T39), H42 (= H42), H59 (= H58), E60 (= E59), D89 (≠ S88), E92 (≠ S91), S95 (= S94), S103 (≠ C102), D150 (= D156), T154 (= T160), K346 (= K349)
- binding acetate ion: P24 (= P24), G69 (= G68), S70 (= S69)
- binding cacodylate ion: S39 (≠ T39), H59 (= H58), W110 (= W116), D150 (= D156)
- binding 1,2-ethanediol: G98 (= G97), Y99 (≠ F98), M101 (≠ A100), H157 (≠ G163)
- binding zinc ion: C37 (= C37), D51 (≠ V50), H53 (≠ K52), H59 (= H58), D150 (= D156)
2ouiA D275p mutant of alcohol dehydrogenase from protozoa entamoeba histolytica (see paper)
37% identity, 99% coverage: 1:349/354 of query aligns to 1:346/360 of 2ouiA
- active site: C37 (= C37), T38 (≠ G38), S39 (≠ T39), H42 (= H42), H59 (= H58), E60 (= E59), D89 (≠ S88), E92 (≠ S91), S95 (= S94), S103 (≠ C102), D150 (= D156), T154 (= T160), K346 (= K349)
- binding zinc ion: C37 (= C37), H59 (= H58), D150 (= D156)
3fsrA Chimera of alcohol dehydrogenase by exchange of the cofactor binding domain res 153-295 of t. Brockii adh by c. Beijerinckii adh (see paper)
36% identity, 99% coverage: 1:349/354 of query aligns to 1:346/352 of 3fsrA
- active site: C37 (= C37), T38 (≠ G38), S39 (≠ T39), H42 (= H42), H59 (= H58), E60 (= E59), D89 (≠ S88), T92 (≠ S91), V95 (≠ S94), S103 (≠ C102), D150 (= D156), T154 (= T160), K346 (= K349)
- binding zinc ion: C37 (= C37), H59 (= H58), D150 (= D156)
1ykfA NADP-dependent alcohol dehydrogenase from thermoanaerobium brockii (see paper)
36% identity, 99% coverage: 1:349/354 of query aligns to 1:346/352 of 1ykfA
- active site: C37 (= C37), T38 (≠ G38), S39 (≠ T39), H42 (= H42), H59 (= H58), E60 (= E59), D89 (≠ S88), T92 (≠ S91), V95 (≠ S94), S103 (≠ C102), D150 (= D156), T154 (= T160), K346 (= K349)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: S39 (≠ T39), D150 (= D156), T154 (= T160), G174 (≠ A180), I175 (≠ Q181), G176 (= G182), P177 (= P183), V178 (≠ I184), S199 (= S205), R200 (≠ I206), Y218 (≠ F224), I223 (≠ P229), N266 (≠ G272), Y267 (≠ V273)
- binding zinc ion: C37 (= C37), H59 (= H58), D150 (= D156)
1bxzB Crystal structure of a thermophilic alcohol dehydrogenase substrate complex from thermoanaerobacter brockii (see paper)
36% identity, 99% coverage: 1:349/354 of query aligns to 1:346/352 of 1bxzB
- active site: C37 (= C37), T38 (≠ G38), S39 (≠ T39), H42 (= H42), H59 (= H58), E60 (= E59), D89 (≠ S88), T92 (≠ S91), V95 (≠ S94), S103 (≠ C102), D150 (= D156), T154 (= T160), K346 (= K349)
- binding 2-butanol: H59 (= H58), D150 (= D156)
P14941 NADP-dependent isopropanol dehydrogenase; EC 1.1.1.80 from Thermoanaerobacter brockii (Thermoanaerobium brockii) (see 2 papers)
36% identity, 99% coverage: 1:349/354 of query aligns to 1:346/352 of P14941
- C37 (= C37) binding Zn(2+)
- H59 (= H58) binding Zn(2+)
- D150 (= D156) binding Zn(2+)
- IGPV 175:178 (≠ QGPI 181:184) binding NADP(+)
- GSR 198:200 (≠ ESI 204:206) binding NADP(+)
- Y218 (≠ F224) binding NADP(+)
- VNY 265:267 (≠ LGV 271:273) binding NADP(+)
- K340 (≠ Q343) binding NADP(+)
7f3pD Crystal structure of a NADP-dependent alcohol dehydrogenase mutant in apo form (see paper)
36% identity, 99% coverage: 1:349/354 of query aligns to 4:349/355 of 7f3pD
7uutA Ternary complex crystal structure of secondary alcohol dehydrogenases from the thermoanaerobacter ethanolicus mutants c295a and i86a provides better understanding of catalytic mechanism (see paper)
36% identity, 99% coverage: 1:349/354 of query aligns to 1:346/352 of 7uutA
- binding (2R)-pentan-2-ol: S39 (≠ T39), H59 (= H58), A85 (= A84), W110 (= W116), D150 (= D156), C295 (= C299)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: C37 (= C37), T38 (≠ G38), S39 (≠ T39), D150 (= D156), T154 (= T160), G174 (≠ A180), G176 (= G182), P177 (= P183), V178 (≠ I184), S199 (= S205), R200 (≠ I206), A242 (= A248), G243 (≠ L249), G244 (= G250), I248 (≠ T254), V265 (≠ L271), N266 (≠ G272), Y267 (≠ V273), C295 (= C299), K340 (≠ Q343)
- binding zinc ion: C37 (= C37), H59 (= H58), D150 (= D156)
7ux4A Crystallographic snapshots of ternary complexes of thermophilic secondary alcohol dehydrogenase from thermoanaerobacter pseudoethanolicus reveal the dynamics of ligand exchange and the proton relay network. (see paper)
37% identity, 94% coverage: 16:349/354 of query aligns to 16:344/350 of 7ux4A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: D148 (= D156), T152 (= T160), G172 (≠ A180), I173 (≠ Q181), G174 (= G182), P175 (= P183), V176 (≠ I184), R198 (≠ I206), G242 (= G250), V263 (≠ L271), N264 (≠ G272), Y265 (≠ V273), C293 (= C299)
- binding (1S,3S)-3-methylcyclohexan-1-ol: S37 (≠ T39), H57 (= H58), W108 (= W116), D148 (= D156)
- binding zinc ion: C35 (= C37), H57 (= H58), E58 (= E59), D148 (= D156)
7xy9A Cryo-em structure of secondary alcohol dehydrogenases tbsadh after carrier-free immobilization based on weak intermolecular interactions (see paper)
36% identity, 94% coverage: 16:349/354 of query aligns to 19:339/344 of 7xy9A
- binding magnesium ion: H101 (= H99), H103 (≠ Q101), H158 (≠ G163), C288 (= C299), G290 (= G301), G291 (= G302), L293 (≠ E304), R294 (= R305)
- binding zinc ion: C38 (= C37), H60 (= H58), E61 (= E59), D151 (= D156)
1kevA Structure of NADP-dependent alcohol dehydrogenase (see paper)
35% identity, 95% coverage: 16:352/354 of query aligns to 16:349/351 of 1kevA