SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_047094455.1 NCBI__GCF_001013305.1:WP_047094455.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 13 hits to proteins with known functional sites (download)

Q28615 Solute carrier family 13 member 2; Na(+)/dicarboxylate cotransporter 1; NaDC-1; Renal sodium/dicarboxylate cotransporter from Oryctolagus cuniculus (Rabbit) (see paper)
31% identity, 90% coverage: 38:491/502 of query aligns to 42:555/593 of Q28615

query
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Q28615

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L83 (≠ Y79) mutation to C: Decreases cell membrane expression. Decreases succinate transport activity. Decreases Km value for succinate.
T86 (≠ I82) mutation to C: Does not affect cell membrane localization. Decreases succinate transport activity.
Y228 (≠ F204) mutation to C: Does not affect cell membrane localization. Decreases succinate transport activity.
Y432 (≠ L363) mutation to C: Does not affect cell membrane localization. Decreases succinate transport activity. Decreases Km value for succinate. More sensitive to inhibition by lithium.
T474 (= T405) mutation to C: Does not affect cell membrane localization. Abolishes succinate transport activity.
N525 (= N461) mutation to C: Decreases cell membrane expression. Decreases succinate transport activity.
M539 (≠ L475) mutation to C: Does not affect cell membrane localization. Decreases succinate transport activity. Insensitive to inhibition by lithium.

8w6gA Nadc1 with inhibitor aca (see paper)
31% identity, 90% coverage: 38:490/502 of query aligns to 42:500/527 of 8w6gA

query
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8w6gA

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binding 2-[[(~{E})-3-(4-pentylphenyl)prop-2-enoyl]amino]benzoic acid: W233 (vs. gap), L237 (vs. gap), A486 (≠ I476), R487 (≠ K477), F490 (≠ I480)
binding cholesterol hemisuccinate: L66 (≠ V62), M69 (≠ F65)

Sites not aligning to the query:

binding cholesterol hemisuccinate: 37, 40

8w6cA Cryoem structure of nadc1 with citrate (see paper)
31% identity, 90% coverage: 38:490/502 of query aligns to 42:503/530 of 8w6cA

query
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8w6cA

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A

F

Y

D

C

L

V

S

I

A

Q

A

T

R

E

K

H

A

R

I

L

A

L

P

-

Q

-

G

G

P

P

W

M

S

T

E

F

A

A

E

E

R

K

R

A

L

I

V

S

P

I

V

L

I

F

A

V

V

I

T

L

F

V

L

L

A

L

W

W

M

F

L

T

R

R

-

E

-

P

G

G

F

F

V

F

A

L

P

G

Y

W

-

G

-

N

-

L

-

A

F

F

P

P

-

N

-

A

-

K

-

G

E

E

G

S

S

M

L

V

T

S

D

D

G

G

T

T

V

V

A

A

I

I

A

F

A

I

A

G

F

I

L

I

-

M

-

F

-

I

-

P

-

S

-

K

-

F

-

P

-

G

L

L

F

T

T

Q

L

D

P

P

D

E

G

N

T

P

G

G

R

K

R

L

-

K

-

A

-

P

-

L

-

G

L

L

E

D

W

W

R

K

E

T

A

V

N

N

-

Q

R

K

A

M

P

P

W

W

D

N

V

I

L

V

M

L

F

L

G

G

L

Y

A

A

L

L

A

A

G

K

A

G

M

S

T

E

R

R

T

S

G

G

L

L

A

S

D

E

W

W

L

L

G

G

N

N

A

K

L

L

L

T

P

P

L

L

A

Q

G

S

V

V

P

P

V

A

I

P

V

A

I

I

A

A

L

I

A

I

L

L

V

S

A

L

M

L

V

V

I

A

L

T

I

F

T
|
T

E

E

F

C

A
x
T

S
|
S

N
|
N

V

V

A

A

T

T

A

T

S

T

G

I

I

F

M

L

P

P

V

I

V

L

A

A

A

S

L

M

A

A

V

Q

A

A

I

I

S

C

G

L

E

H

S

P

G

-

N

-

V

-

L

L

L

Y

L

V

A

M

M

L

P

P

V

C

A

T

L

L

A

A

A

T

S

S

W

L

G

A

F

F

V

M

L

L

P

P

A

V

G
x
A

T

T

G

P

P

P

N

N

A

A

I

I

A

V

W

F

S

S

T

F

G

G

R

D

I

L

A

K

L

V

P

L

R

D

L

M

I

A

K

R

A

A

G

G

I

F

T

L

L

L

D

N

I

I

A

I

G

G

I

V

F

L

L

V

I

I

binding sodium ion: S136 (= S134), I139 (= I137), S140 (= S138), N141 (= N139), G191 (= G216), T423 (= T405), T426 (≠ A408), S427 (= S409), N428 (= N410), A470 (≠ G457)
binding cholesterol hemisuccinate: I65 (≠ A61), M69 (≠ F65)

Sites not aligning to the query:

binding cholesterol hemisuccinate: 37, 40

7jsjA Structure of the nact-pf2 complex (see paper)
30% identity, 91% coverage: 33:490/502 of query aligns to 25:446/468 of 7jsjA

query
sites
7jsjA

N

R

T

C

A

A

G

Y

L

V

V

I

M

I

W

L

M

M

A

A

T

I

W

Y

W

W

M

C

T

T

Q

E

A

V

I

I

P

P

L

L

T

A

A

V

T

T

G

S

L

L

L

M

P

P

F

V

A

L

V

L

L

F

P

P

F

L

V

F

S

Q

A

I

G

L

T

D

A

S

N

R

E

Q

V

V

A

C

G

V

D

Q

Y

Y

Y

M

S

K

P

D

I

T

I

N

F

M

L

L

L

F

L

L

G

G

A

L

F

I

L

V

A

A

L

V

A

A

I

V

E

E

R

R

T

W

G

N

L

L

H

H

K

K

R

R

L

I

A

A

C

L

W

R

I

T

L

L

D

L

R

W

I

V

G

G

G

A

R

K

G

P

G

A

E

R

F

-

G

-

L

L

L

M

L

L

G

G

F

F

M

M

I

G

A

V

A

T

A

A

I

L

L

L

S
|
S

N

M

I

W

I
|
I

S
|
S

N
|
N

T
|
T

S

A

T

T

A

T

L

A

I

M

M

M

M

V

P

P

M

I

A

V

L

E

A

A

V

I

L

L

S

-

G

-

A

-

S

-

C

-

N

-

E

-

V

-

I

-

G

-

Q

-

K

-

G

-

S

-

T

-

S

-

C

-

N

-

E

-

V

-

I

-

G

-

Q

Q

K

Q

E

R

S

K

R

R

A

-

D

-

G

-

T

-

L

-

D

-

Q

-

S

-

G

-

L

L

S

C

G

K

A

A

L

M

P

T

M

L

G

C

L

I

A

C

F

Y

A

A

S

S

I

I

G

G

L

T

G

A

T

T

I

L

I

T

G
|
G

S
x
T

P

G

T

P

N

N

A

V

I

V

G

L

V

L

A

G

L

Q

L

M

R

N

D

E

I

L

-

F

-

P

-

D

S

S

G

K

I

D

E

L

I

V

T

N

F

F

A

A

Q

S

W

W

A

F

M

A

F

F

G

A

V

F

P

P

I

N

V

M

I

L

A

V

G

M

I

L

P

L

L

F

A

A

A

W

V

L

I

W

I

L

A

Q

K

F

V

V

Q

Y

R

M

I

R

A

S

D

K

H

K

P

-

F

-

D

N

L

E

S

K

A

A

R

L

K

K

A

V

I

L

A

Q

P

E

Q

E

-

Y

-

R

-

K

-

L

G

G

P

P

W

L

S

S

E

F

A

A

E

E

R

I

R

N

L

V

V

L

P

I

V

C

I

F

A

F

V

L

T

L

F

V

L

I

A

L

W

W

M

F

L

S

R

R

G

D

F

-

V

-

A

-

P

-

Y

-

F

-

P

P

E

E

G

T

S

K

-

Y

L

V

T

S

D

D

G

A

T

T

V

V

A

A

I

I

A

F

A

V

A

A

F

T

L

L

F

F

T

I

L

V

P

P

D

S

G

Q

T

K

G

P

R

K

R

F

-

Y

-

P

L

L

E

D

W

W

R

K

-

V

E

T

A

Q

N

E

R

K

A

V

P

P

W

W

D
x
G

V
x
I

L

V

M

L

F

L

G

G

L

F

A

A

L

L

A

A

G

K

A

G

M

S

T

E

R

A

T

S

G

G

L

L

A

S

D

V

W

W

L

M

G

G

N

K

A

Q

L

M

L

E

P

P

L

L

A

H

G

A

V

V

P

P

V

P

I

A

V

A

I

I

A

T

L

L

A

I

L

L

V

S

A

L

M

L

V

V

I

A

L

V

I

F

T
|
T

E

E

F

C

A
x
T

S
|
S

N
|
N

V

V

A

A

T

T

A

T

S

T

G

L

I

F

M

L

P

P

V

I

V

F

A

A

A

S

L

M

A

S

V

R

A

S

I

I

S

-

G

-

E

-

S

-

G

-

G

G

N

L

V

N

V

P

L

L

L

Y

L

I

A

M

M

L

P

P

V

C

A

T

L

L

A

S

A

A

S

S

W

F

G

A

F

F

V

M

L

L

P

P

A

V

G
x
A

T
|
T

G

P

P

P

N

N

A

A

I

I

A

V

W

F

S

T

T

Y

G

G

R

H

I

L

A

K

L

V

P

A

R

D

L

M

I

V

K

K

A

T

G

G

I

V

T

I

L

M

D

N

I

I

A

I

G

G

I

V

F

F

L

C

I

V

binding sodium ion: S124 (= S134), I127 (= I137), S128 (= S138), N129 (= N139), G172 (= G216), T366 (= T405), T369 (≠ A408), S370 (= S409), N371 (= N410), A413 (≠ G457), T414 (= T458)
binding (2R)-2-[2-(4-tert-butylphenyl)ethyl]-2-hydroxybutanedioic acid: N129 (= N139), T130 (= T140), T173 (≠ S217), G315 (≠ D354), I316 (≠ V355), N371 (= N410)

Q86YT5 Na(+)/citrate cotransporter; NaCT; Sodium-coupled citrate transporter; Sodium-dependent citrate transporter; Solute carrier family 13 member 5 from Homo sapiens (Human) (see 5 papers)
29% identity, 91% coverage: 33:490/502 of query aligns to 37:540/568 of Q86YT5

query
sites
Q86YT5

N

R

T

C

A

A

G

Y

L

V

V

I

M

I

W

L

M

M

A

A

T

I

W

Y

W

W

M

C

T

T

Q

E

A

V

I

I

P

P

L

L

T

A

A

V

T

T

G

S

L

L

L

M

P

P

F

V

A

L

V

L

L

F

P

P

F

L

V

F

S

Q

A

I

G

L

T

D

A

S

N

R

E

Q

V

V

A

C

G

V

D

Q

Y

Y

Y

M

S

K

P

D

I

T

I

N

F

M

L

L

L

F

L

L

G

G

A

L

F

I

L

V

A

A

L

V

A

A

I

V

E

E

R

R

T

W

G

N

L

L

H

H

K

K

R

R

L

I

A

A

C

L

W

R

I

T

L

L

D

L

R

W

I

V

G

G

G

A

R

K

G

P

G

A

E

R

F

-

G

-

L

L

L

M

L

L

G

G

F

F

M

M

I

G

A

V

A

T

A

A

I

L

L

L

S

S

N

M

I

W

I

I

S

S

N

N

T
|
T

S

A

T

T

A

T

L

A

I

M

M

M

M

V

P

P

M

I

A

V

L

E

A

A

V

I

L

L

S

Q

G

Q

G

M

A

E

A

A

S

T

S

S

C

A

N

A

E

T

V

E

I

A

G

G

Q

L

K

E

-

L

-

V

-

D

-

K

-

G

-

K

-

A

-

K

-

E

-

L

-

P

G

G

S

S

T

Q

S

V

C

I

N

F

E

E

-

G

-

P

V

T

I

L

G

G

Q

Q

K

Q

E

E

S

D

R

Q

A

-

D

-

G

-

T

-

L

-

D

E

Q

R

S

K

G

R

L

L

S

C

G

K

A

A

L

M

P

T

M

L

G

C

L

I

A

C

F

Y

A

A

S

S

I

I

G
|
G

G

G

L

T

G

A

T

T

I

L

I

T

G

G

S
x
T

P

G

T

P

N

N

A

V

I

V

G

L

V

L

A

G

L

Q

L

M

R

N

D

E

I

L

-

F

-

P

-
x
D

S

S

G

K

I

D

E

L

I

V

T

N

F

F

A

A

Q

S

W

W

A

F

M

A

F

F

G

A

V

F

P

P

I

N

V

M

I

L

A

V

G

M

I

L

P

L

L

F

A

A

A

W

V

L

I

W

I

L

A

Q

K

F

V

V

-

Y

-

M

-

R

-

F

-

N

-

F

-

K

-

S

-

W

-

G

-

C

-

G

-

L

Q

E

R

S

I

K

A

K

D

N

H

E

P

K

F

A

D

A

L

L

S

K

A

V

A

L

R

Q

K

E

A

E

I

Y

A

R

P

K

Q

L

G

G

P

P

W

L

S

S

E

F

A

A

E

E

R

I

R

N

L

V

V

L

P

I

V

C

I

F

A

F

V

L

T

L

F

V

L

I

A

L

W

W

M

F

L

S

R

R

-

D

-

P

-

G

-

F

-

M

-

P

G

G

F
x
W

V
x
L

A
x
T

P
x
V

Y
x
A

F
x
W

P
x
V

E
|
E

G
|
G

S
x
E

-
x
T

-
x
K

-
x
Y

L
x
V

T
x
S

D
|
D

G
x
A

T
|
T

V
|
V

A
|
A

I
|
I

A
x
F

A
x
V

A
|
A

F
x
T

L
|
L

F
|
F

T
x
I

L
x
V

P
|
P

D
x
S

G
x
Q

T
x
K

G
x
P

R
x
K

R
x
F

-
x
N

-
x
F

-
x
R

-
x
S

-
x
Q

-
x
T

-
x
E

-
x
R

-
x
K

-
x
T

-
x
P

-
x
F

-
x
Y

-
x
P

L
|
L

E
x
D

W
|
W

R
x
K

-
x
V

E
x
T

A
x
Q

N
x
E

R
x
K

A
x
V

P
|
P

W
|
W

D
x
G

V
x
I

L
x
V

M
x
L

F
x
L

G
|
G

L
x
F

A
|
A

L
|
L

A
|
A

G
x
K

A
x
G

M
x
S

T
x
E

R
x
A

T
x
S

G
|
G

L
|
L

A
x
S

D
x
V

W
|
W

L
x
M

G
|
G

N
x
K

A
x
Q

L
x
M

L
x
E

P
|
P

L
|
L

A
x
H

G
x
A

V
|
V

P
|
P

V
x
P

I
x
A

V
x
A

I
|
I

A
x
T

L
|
L

A
x
I

L
|
L

V
x
S

A
x
L

M
x
L

V
|
V

I
x
A

L
x
V

I
x
F

T
|
T

E
|
E

F
x
C

A
x
T

S
|
S

N
|
N

V
|
V

A
|
A

T
|
T

A
x
T

S
x
T

G
x
L

I
x
F

M
x
L

P
|
P

V
x
I

V
x
F

A
|
A

A
x
S

L
x
M

A
x
S

V
x
R

A
x
S

I
|
I

S

-

G

-

E

-

S

-

G

-

G
|
G

N
x
L

V
x
N

V
x
P

L
|
L

L
x
Y

L
x
I

A
x
M

M
x
L

P
|
P

V
x
C

A
x
T

L
|
L

A
x
S

A
|
A

S
|
S

W
x
F

G
x
A

F
|
F

V
x
M

L
|
L

P
|
P

A
x
V

G
x
A

T
|
T

G
x
P

P
|
P

N
|
N

A
|
A

I
|
I

A
x
V

W
x
F

S
x
T

T
x
Y

G
|
G

R
x
H

I
x
L

A
x
K

L
x
V

P
x
A

R
x
D

L
x
M

I
x
V

K
|
K

A
x
T

G
|
G

I
x
V

T
x
I

L
x
M

D
x
N

I
|
I

A
x
I

G
|
G

I
x
V

F
|
F

L
x
C

I
x
V

T142 (= T140) to M: in DEE25; no loss of localization to plasma membrane; loss of function in citrate transport; dbSNP:rs761917087
G219 (= G209) to E: loss of localization to plasma membrane; loss of function in citrate transport; dbSNP:rs150024888; to R: in DEE25; loss of function in citrate transport; loss of localization to plasma membrane; dbSNP:rs144332569
T227 (≠ S217) to M: in DEE25; loss of function in citrate transport; no effect on localization to plasma membrane; dbSNP:rs587777577
D243 (vs. gap) to N: no effect on localization to plasma membrane; no effect on its function in citrate transport; dbSNP:rs142262032
G409 (≠ D354) mutation to Q: No effect on its function in citrate transport.
I410 (≠ V355) mutation I->A,F: Significant loss of function in citrate transport.; mutation to V: No effect on its function in citrate transport.
L420 (= L365) to P: loss of localization to plasma membrane; loss of function in citrate transport; dbSNP:rs150738356
S427 (≠ T372) to L: in DEE25; loss of localization to plasma membrane; loss of function in citrate transport; dbSNP:rs548065551
L485 (≠ N435) to R: no effect on localization to plasma membrane; reduced function in citrate transport; increased Km and Vmax values compared with that of wild type with citrate as substrate; dbSNP:rs148049520
L488 (= L438) to P: in DEE25; loss of function in citrate transport; loss of localization to plasma membrane; dbSNP:rs587777578
D524 (≠ R474) to H: in DEE25; loss of function in citrate transport; no effect on localization to plasma membrane; dbSNP:rs863225448

Sites not aligning to the query:

341:568 natural variant: Missing (in DEE25; loss of localization to plasma membrane; loss of function in citrate transport)
562 modified: carbohydrate, N-linked (GlcNAc...) asparagine

8w6nB Nas1 with sulfate in in/out state (see paper)
31% identity, 89% coverage: 41:487/502 of query aligns to 37:455/481 of 8w6nB

query
sites
8w6nB

M

V

A

A

T

T

W

F

W

W

M

L

T

T

Q

E

A

A

I

L

P

P

L

L

T

S

A

V

T

T

G

A

L

L

P

P

F

S

A

L

V

M

L

L

P

P

F

M

V

F

S

G

A

I

G

M

T

P

A

S

N

K

E

K

V

V

A

A

G

S

D

A

Y

Y

Y

F

S

K

P

D

I

F

I

H

F

L

L

L

L

I

G

G

G

V

A

I

F

C

L

L

A

A

L

T

A

S

I

I

E

E

R

K

T

W

G

N

L

L

H

H

K

K

R

R

L

I

A

A

C

L

W

K

I

M

L

V

D

M

R

M

I

V

G

G

G

V

R

N

G

P

G

A

E

-

F

-

G

W

L

L

L

T

L

L

G

G

F

F

M

M

I

S

A

S

A

T

A

A

I

F

L

L

S

S

N

M

I

W

I

L

S

S

N
|
N

T
|
T

S

S

T

T

A

A

L

A

I

M

M

V

M

M

P

P

M

I

A

A

L

E

A

A

V

V

L

V

S

-

G

-

A

-

S

-

C

-

N

-

E

-

V

-

I

-

G

-

Q

-

K

-

G

-

S

-

T

-

S

-

C

-

N

Q

E

Q

V

I

I

I

G

N

Q

A

K

G

E

H

S

V

R

T

A

R

D

K

G

L

T

T

L

C

D

-

Q

-

S

-

G

-

L

-

S

-

G

-

A

-

L

-

P

-

M

L

G

C

L

I

A

A

F

Y

A

S

S

T

I

I

G

G

L

L

G

T

T

T

I

I

I

T

G

G

S
x
T

P
x
S

T

T

N

N

A

L

I

I

G

F

V

A

A

E

L

Y

L

F

-

N

-

T

R

R

D

Y

I

P

S

D

G

C

I

R

E

C

I

L

T

N

F

F

A

G

Q

S

W

W

A

F

M

T

F

F

G

S

V

F

P

P

-

A

-

L

-

I

I

L

V

L

I

L

A

S

G

W

I

I

P

W

L

L

A

Q

A

W

V

L

I

F

I

L

A

G

K

Q

V

Q

Q

K

R

A

I

C

A

A

D

E

H

-

P

-

F

-

D

-

L

-

S

-

A

V

A

I

R

K

K

Q

A

E

I

Y

A

Q

P

K

Q

L

G

G

P

P

W

I

S

R

E

Y

A

Q

E

E

R

I

R

V

L

T

V

L

P

V

V

L

I

F

A

I

V

I

T

M

F

A

L

L

A

L

W

W

M

F

L

S

R

R

-

D

-

P

G

G

F

F

V

V

-

P

-

G

-

W

A

S

P

A

Y

L

F

F

P

S

E

E

-

Y

-

P

G

G

S

F

L

A

T

T

D

D

G

S

T

T

V

V

A

A

I

L

A

L

A

I

A

G

F

L

L

L

L

F

F

F

T

L

L

I

P

P

D

A

G

K

T

T

G

Y

R

S

R

P

L

L

L

I

E

T

W

W

R

K

E

E

A

F

N

Q

R

S

-

F

A

M

P

P

W

W

D

D

V

I

L

A

M

I

M

L

F

V

G

G

L

F

A

A

L

L

A

A

G

D

A

G

M

C

T

E

R

E

T

S

G

G

L

L

A

S

D

K

W

W

L

I

G

G

N

N

A

K

L

L

L

S

P

P

L

L

A

G

G

S

V

L

P

P

V

A

I

W

V

L

I

I

A

I

L

L

A

I

L

S

V

S

A

L

M

M

V

V

I

T

L

S

I

L

T

T

E

E

F

V

A

A

S

S

N

N

V

P

A

A

T

T

A

I

S

T

G

L

I

F

M

L

P

P

V

I

V

L

A

S

A

P

L

L

A

A

V

E

A

A

I

I

S

H

G

V

E

N

S

P

G

-

N

-

V

-

L

L

L

Y

L

I

A

L

M

I

P

P

V

S

A

T

L

L

A

C

A

T

S

S

W

F

G

A

F

F

V

L

L

L

P

P

A

V

G

A

T

N

G

P

P

P

N

N

A

A

I

I

A

V

W

F

S

S

T

Y

G

G

R

H

I

L

A

K

L

V

P

I

R

D

L

M

I

V

K

K

A

A

G

G

I

L

T

G

L

V

D

N

I

I

A

V

G

G

I

V

binding sulfate ion: N133 (= N139), T134 (= T140), T181 (≠ S217), S182 (≠ P218)

8w6nA Nas1 with sulfate in in/out state (see paper)
32% identity, 89% coverage: 41:487/502 of query aligns to 41:467/494 of 8w6nA

query
sites
8w6nA

M

V

A

A

T

T

W

F

W

W

M

L

T

T

Q

E

A

A

I

L

P

P

L

L

T

S

A

V

T

T

G

A

L

L

P

P

F

S

A

L

V

M

L

L

P

P

F

M

V

F

S

G

A

I

G

M

T

P

A

S

N

K

E

K

V

V

A

A

G

S

D

A

Y

Y

Y

F

S

K

P

D

I

F

I

H

F

L

L

L

L

I

G

G

G

V

A

I

F

C

L

L

A

A

L

T

A

S

I

I

E

E

R

K

T

W

G

N

L

L

H

H

K

K

R

R

L

I

A

A

C

L

W

K

I

M

L

V

D

M

R

M

I

V

G

G

G

V

R

N

G

P

G

A

E

-

F

-

G

W

L

L

L

T

L

L

G

G

F

F

M

M

I

S

A

S

A

T

A

A

I

F

L

L

S
|
S

N

M

I

W

I

L

S

S

N
|
N

T

T

S

S

T

T

A

A

L

A

I

M

M

V

M

M

P

P

M

I

A

A

L

E

A

A

V

V

L

V

S

-

G

-

A

-

S

-

C

-

N

-

E

-

V

-

I

-

G

-

Q

-

K

-

G

-

S

-

T

-

S

-

C

-

N

-

E

Q

V

Q

I

I

G

I

Q

N

K

A

E

E

S

A

R

K

A

G

D

H

G

V

T

T

L

R

D

K

Q

L

S

T

G

C

L

L

S

C

G

-

A

-

L

-

P

-

M

-

G

-

L

I

A

A

F

Y

A

S

S

T

I

I

G

G

G
|
G

L

L

G

T

T

T

I

I

I

T

G
|
G

S
x
T

P

S

T

T

N

N

A

L

I

I

G

F

V

A

A

E

L

Y

L

F

-

N

-

T

R

R

D

Y

I

P

S

D

G

C

I

R

E

C

I

L

T

N

F

F

A

G

Q

S

W

W

A

F

M

T

F

F

G

S

V

F

P

P

-

A

-

L

-

I

I

L

V

L

I

L

A

S

G

W

I

I

P

W

L

L

A

Q

A

W

V

L

I

F

I

L

A

G

K

T

V

V

Q

Q

R

Q

I

K

A

A

D

C

H

A

P

-

F

-

D

-

L

-

S

E

A

V

A

I

R

K

K

Q

A

E

I

Y

A

Q

P

K

Q

L

G

G

P

P

W

I

S

R

E

Y

A

Q

E

E

R

I

R

V

L

T

V

L

P

V

V

L

I

F

A

I

V

I

T

M

F

A

L

L

A

L

W

W

M

F

L

S

R

R

-

D

-

P

G

G

F

F

V

V

-

P

-

G

-

W

A

S

P

A

Y

L

F

F

P

S

E

E

-

Y

-

P

G

G

S

F

L

A

T

T

D

D

G

S

T

T

V

V

A

A

I

L

A

L

A

I

A

G

F

L

L

L

L

F

F

F

T

L

L

I

P

P

D

A

G

K

T

T

-

L

-

F

-

D

G

Y

R

S

R

P

L

L

L

I

E

T

W

W

R

K

E

E

A

F

N

Q

R

S

-

F

A

M

P

P

W

W

D

D

V

I

L

A

M

I

M

L

F

V

G

G

L

F

A

A

L

L

A

A

G

D

A

G

M

C

T

E

R

E

T

S

G

G

L

L

A

S

D

K

W

W

L

I

G

G

N

N

A

K

L

L

L

S

P

P

L

L

A

G

G

S

V

L

P

P

V

A

I

W

V

L

I

I

A

I

L

L

A

I

L

S

V

S

A

L

M

M

V

V

I

T

L

S

I

L

T

T

E

E

F

V

A

A

S

S

N

N

V

P

A

A

T

T

A

I

S

T

G

L

I

F

M

L

P

P

V

I

V

L

A

S

A

P

L

L

A

A

V

E

A

A

I

I

S

H

G

V

E

N

S

P

G

-

N

-

V

-

L

L

L

Y

L

I

A

L

M

I

P

P

V

S

A

T

L

L

A

C

A

T

S

S

W

F

G

A

F

F

V

L

L

L

P

P

A

V

G

A

T

N

G

P

P

P

N

N

A

A

I

I

A

V

W

F

S

S

T

Y

G

G

R

H

I

L

A

K

L

V

P

I

R

D

L

M

I

V

K

K

A

A

G

G

I

L

T

G

L

V

D

N

I

I

A

V

G

G

I

V

binding sodium ion: S132 (= S134), N137 (= N139), G181 (= G210), G187 (= G216), T188 (≠ S217)

8w6hA Nas1 with sulfate - in/in state (see paper)
31% identity, 89% coverage: 41:487/502 of query aligns to 36:436/462 of 8w6hA

query
sites
8w6hA

M

V

A

A

T

T

W

F

W

W

M

L

T

T

Q

E

A

A

I

L

P

P

L

L

T

S

A

V

T

T

G

A

L

L

P

P

F

S

A

L

V

M

L

L

P

P

F

M

V

F

S

G

A

I

G

M

T

P

A

S

N

K

E

K

V

V

A

A

G

S

D

A

Y

Y

Y

F

S

K

P

D

I

F

I

H

F

L

L

L

L

I

G

G

G

V

A

I

F

C

L

L

A

A

L

T

A

S

I

I

E

E

R

K

T

W

G

N

L

L

H

H

K

K

R

R

L

I

A

A

C

L

W

K

I

M

L

V

D

M

R

M

I

V

G

G

G

V

R

N

G

P

G

A

E

-

F

-

G

W

L

L

L

T

L

L

G

G

F

F

M

M

I

S

A

S

A

T

A

A

I

F

L

L

S
|
S

N

M

I

W

I
x
L

S
|
S

N
|
N

T
|
T

S

S

T

T

A

A

L

A

I

M

M

V

M

M

P

P

M

I

A

A

L

E

A

A

V

V

L

V

S

-

G

-

A

-

S

-

C

-

N

-

E

-

V

-

I

-

G

-

Q

-

K

-

G

-

S

-

T

-

S

-

C

-

N

Q

E

Q

V

I

I

I

G

N

Q

A

K

G

E

H

S

V

R

T

A

R

D

K

G

L

T

T

L

C

D

-

Q

-

S

-

G

-

L

-

S

-

G

-

A

-

L

-

P

-

M

L

G

C

L

I

A

A

F

Y

A

S

S

T

I

I

G

G

L

L

G

T

T

T

I

I

I

T

G
|
G

S
x
T

P
x
S

T

T

N

N

A

L

I

I

G

F

V

A

A

E

L

Y

L

F

-

N

-

T

R

R

D

Y

I

P

S

D

G

C

I

R

E

C

I

L

T

N

F

F

A

G

Q

S

W

W

A

F

M

T

F

F

G

S

V

F

P

P

I

-

V

-

I

-

A

-

G

-

I

-

P

-

L

-

A

A

V

L

I

I

A

L

K

L

V

L

Q

S

R

W

I

I

A

W

D

L

H

Q

P

W

F

L

D

F

L

L

S

-

A

G

A

I

R

K

K

Q

A

E

I

Y

A

Q

P

K

Q

L

G

G

P

P

W

I

S

R

E

Y

A

Q

E

E

R

I

R

V

L

T

V

L

P

V

V

L

I

F

A

I

V

I

T

M

F

A

L

L

A

L

W

W

M

F

L

S

R

R

G

-

F

-

V

-

A

-

P

-

Y

-

F

-

P

-

E

P

G

G

S

F

L

A

T

T

D

D

G

S

T

T

V

V

A

A

I

L

A

L

A

I

A

G

F

L

L

L

L

F

F

F

T

L

L

I

P

P

D

A

G

K

T

T

-

L

-

T

-

A

-

F

-

D

G

Y

R

S

R

P

L

L

L

I

E

T

W

W

R

K

E

E

A

F

N

Q

R

S

-

F

A

M

P

P

W

W

D

D

V

I

L

A

M

I

M

L

F

V

G

G

L

F

A

A

L

L

A

A

G

D

A

G

M

C

T

E

R

E

T

S

G

G

L

L

A

S

D

K

W

W

L
x
I

G

G

N

N

A

K

L

L

L

S

P

P

L

L

A

G

G

S

V

L

P

P

V

A

I

W

V

L

I

I

A

I

L

L

A

I

L

S

V

S

A

L

M

M

V

V

I

T

L

S

I

L

T

T

E

E

F

V

A

A

S

S

N

N

V

P

A

A

T

T

A

I

S

T

G

L

I

F

M

L

P
|
P

V

I

V

L

A

S

A

P

L

L

A

A

V

E

A

A

I

I

S

H

G

V

E

N

S

P

G

-

N

-

V

-

L

L

L

Y

L

I

A

L

M

I

P

P

V

S

A

T

L

L

A

C

A

T

S

S

W

F

G

A

F

F

V

L

L

L

P

P

A

V

G

A

T

N

G

P

P

P

N

N

A

A

I

I

A

V

W

F

S

S

T

Y

G

G

R

H

I

L

A

K

L

V

P

I

R

D

L

M

I

V

K

K

A

A

G

G

I

L

T

G

L

V

D

N

I

I

A

V

G

G

I

V

binding cholesterol: I332 (≠ L378), P373 (= P419)
binding sodium ion: S127 (= S134), L130 (≠ I137), S131 (= S138), N132 (= N139), G179 (= G216), T180 (≠ S217)
binding sulfate ion: T133 (= T140), S181 (≠ P218)

6okzB Structure of vcindy bound to fumarate
30% identity, 90% coverage: 40:493/502 of query aligns to 36:438/444 of 6okzB

query
sites
6okzB

W

F

M

I

A

A

T

V

W

L

W

W

M

L

T

T

Q

E

A

A

I

L

P

H

L

V

T

T

A

V

T

T

G

A

L

I

L

L

P

V

F

P

A

V

V

M

L

A

P

V

F

F

V

F

S

G

A

I

G

F

T

E

A

T

N

Q

E

A

V

A

A

L

G

N

D

N

Y

F

Y

A

S

N

P

S

I

I

F

F

L

L

L

F

L

L

G

G

A

F

F

A

L

L

A

A

L

A

A

A

I

M

E

H

R

H

T

Q

G

G

L

L

H

D

K

K

R

V

L

I

A

A

C

D

W

K

I

V

L

L

D

A

R

M

I

A

G

Q

G

G

R

K

G

M

G

S

-

V

E

A

F

V

G

F

L

M

L

L

L

F

G

G

F

-

M

-

I

-

A

V

A

T

A

A

I

L

L

L

S

S

N

M

I

W

I

I

S

S

N
|
N

T

T

S

A

T

T

A

A

L

A

I

M

M

M

M

L

P

P

M

L

A

V

L

L

A

G

V

V

L

L

S

-

G

-

A

-

S

-

C

-

N

-

E

-

V

-

I

-

G

-

Q

-

K

-

G

-

S

-

T

-

S

-

C

-

N

-

E

-

V

-

I

-

G

-

Q

-

K

-

E

-

S

S

R

K

A

V

D

D

G

A

T

D

L

K

D

Q

Q

R

S

S

G

T

L

Y

S

V

G

F

A

V

L

L

P

-

M

L

G

G

L

V

A

A

F

Y

A

S

A

A

S

S

I

I

G

G

L

I

G

A

T

T

I

L

I

V

G

G

S
|
S

P
|
P

T

P

N

N

A

A

I

I

G

A

V

A

A

A

L

-

R

-

D

-

I

-

S

-

G

E

I

V

E

G

I

L

T

S

F

F

A

T

Q

D

W

W

A

M

M

K

F

F

G

G

V

L

P

P

I

T

V

A

I

M

A

M

G

M

I

L

P

P

L

M

A

A

-

I

A

A

V

I

I

L

I

Y

A

F

K

L

V

L

Q

K

R

P

I

T

A

L

D

N

H

G

P

M

F

F

D

E

L

L

S

D

A

R

A

-

R

-

K

-

A

-

I

-

A

A

P

P

Q

V

G

N

P

-

W

W

S

D

E

K

A

G

E

K

R

V

L

T

V

L

P

G

V

I

I

F

A

G

V

L

T

T

F

V

L

F

A

L

W

W

M

I

L

F

R

S

G

S

F

P

V

I

A

N

P

A

Y

A

F

L

P

G

E

G

G

F

S

K

L

S

T

F

D

D

G

T

T

L

V

V

A

A

I

L

A

G

A

A

A

I

F

L

L

M

L

L

F

-

T

-

L

-

P

-

D

-

G

-

T

S

G

F

R

A

R

R

L

V

E

H

W

W

R

K

E

E

A

I

N

Q

R

K

-

T

A

A

P

D

W

W

D

G

V

V

L

L

M

L

F

F

G

G

L

L

A

C

L

L

A

S

G

N

A

V

M

L

T

K

R

Q

T

T

G

G

L

T

A

S

D

V

W

F

L

L

G

A

N

N

A

A

L

L

L

S

P

D

L

M

-

V

A

S

G

H

V

M

P

G

V

I

I

F

V

V

I

V

A

I

L

L

A

V

L

V

V

A

A

T

M

F

V

V

I

V

L

F

I

L

T

T

E

E

F

F

A

A

S
|
S

N
|
N

V
x
T

A

A

T

S

A

A

S

A

G

L

I

L

M

I

P

P

V

V

V

F

A

A

A

T

L

V

A

A

V

-

A

-

I

-

S

-

G

-

E

E

S

A

G

F

G

G

N

M

V

S

V

P

L

V

L

L

A

S

M

V

P

L

V

I

A

A

L

V

A

A

S

S

W

C

G

A

F

F

V

M

L

L

P

P

A

V

G

A

T

T

G

P

P

P

N

N

A

A

I

I

A

V

W

F

S

A

T

S

G

G

R

H

I

I

A

K

L

Q

P

S

R

E

L

M

I

M

K

R

A

V

G

G

I

L

T

Y

L

L

D

N

I

I

A

A

G

C

I

I

F

G

L

L

I

L

V

T

G

A

V

I

binding fumaric acid: N133 (= N139), S182 (= S217), P183 (= P218), S359 (= S409), N360 (= N410), T361 (≠ V411)

7t9gA Structure of vcindy-na+ (see paper)
30% identity, 90% coverage: 40:493/502 of query aligns to 37:439/445 of 7t9gA

query
sites
7t9gA

W

F

M

I

A

A

T

V

W

L

W

W

M

L

T

T

Q

E

A

A

I

L

P

H

L

V

T

T

A

V

T

T

G

A

L

I

L

L

P

V

F

P

A

V

V

M

L

A

P

V

F

F

V

F

S

G

A

I

G

F

T

E

A

T

N

Q

E

A

V

A

A

L

G

N

D

N

Y

F

Y

A

S

N

P

S

I

I

F

F

L

L

L

F

L

L

G

G

A

F

F

A

L

L

A

A

L

A

A

A

I

M

E

H

R

H

T

Q

G

G

L

L

H

D

K

K

R

V

L

I

A

A

C

D

W

K

I

V

L

L

D

A

R

M

I

A

G

Q

G

G

R

K

G

M

G

S

-

V

E

A

F

V

G

F

L

M

L

L

L

F

G

G

F

-

M

-

I

-

A

V

A

T

A

A

I

L

L

L

S
|
S

N

M

I

W

I

I

S
|
S

N
|
N

T

T

S

A

T

T

A

A

L

A

I

M

M

M

M

L

P

P

M

L

A

V

L

L

A

G

V

V

L

L

S

-

G

-

A

-

S

-

C

-

N

-

E

-

V

-

I

-

G

-

Q

-

K

-

G

-

S

-

T

-

S

-

C

-

N

-

E

-

V

-

I

-

G

-

Q

-

K

-

E

-

S

S

R

K

A

V

D

D

G

A

T

D

L

K

D

Q