SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_047212383.1 NCBI__GCF_001931675.1:WP_047212383.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5lh9D Amine transaminase crystal structure from an uncultivated pseudomonas species in the plp-bound (internal aldimine) form
58% identity, 98% coverage: 3:440/447 of query aligns to 5:445/449 of 5lh9D

query
sites
5lh9D

Y

Y

E

K

N

N

S

A

-

E

-

K

R

K

F

F

W

W

H

H

P

P

M

M

M

G

H

S

P

S

A

A

A

A

M

P

K

H

Q

R

R

D

K

K

P

T

L

L

R

V

I

I

V

A

R

R

G

G

E

D

G

G

S

N

Y

Y

V

I

F

T

D

D

D

I

A

D

G

G

S

Q

K

R

F

M

V

L

D

D

G

G

V

V

G

G

G

G

L

L

W

W

N

N

V

V

N

N

V

I

G

G

H

H

N

N

R

R

P

A

E

S

V

V

K

K

A

A

A

A

I

I

V

A

K

A

Q

Q

L

L

D

D

Q

E

L

L

E

A

Y

Y

F

Y

Q

Q

L

T

F

F

D

D

G

G

V

I

S

A

N

H

P

P

R

R

A

V

E

F

E

D

L

L

S

A

A

E

R

R

L

L

I

T

D

G

V

M

L

F

A

A

P

Q

E

E

D

R

M

M

Q

A

R

R

V

V

V

L

F

F

S

S

S

S

N

G

G
|
G

S
|
S

D

D

A

A

T

V

E

E

T

T

A

A

L

L

K

K

L

M

A

A

R

R

Q

Q

Y

Y

W

W

K

I

V

A

M

S

G

G

Q

E

K

P

D

G

R

R

T

T

K

R

F

F

I

L

S

S

L

L

K

R

E

N

G

G

Y
|
Y

H
|
H

G
|
G

S

V

H

H

F

M

G

G

S

G

A

T

A

S

V

V

N

G

G

G

N

N

M

G

V

V

F

Y

R

H

R

Y

N

N

Y

H

E

G

P

Q

V

L

M

L

P

A

G

G

C

C

S

H

H

L

V

L

Q

D

N

T

P

P

W

W

V

L

Y

Y

R

R

N

N

P

P

F

W

-

D

T

C

H

R

D

D

P

P

E

Q

Q

A

L

L

A

T

V

A

L

H

C

C

A

I

Q

R

L

Q

L

L

E

E

R

E

E

Q

I

I

E

A

F

L

Q

L

G

G

P

A

D

Q

T

T

V

I

A

A

A

A

F

L

I

I

A

A

E
|
E

P

P

V

V

Q

Q

G

G

A

A

G

G

G

G

V

V

I

I

V

V

P

P

P

P

A

A

S

D

Y

Y

W

W

P

P

M

R

V

L

R

R

Q

E

V

V

C

C

D

D

K

R

Y

H

G

G

V

I

L

L

L

L

I

I

A

A

D
|
D

E

E

V
|
V

V

V

T

T

G

G

F

F

G

G

R

R

T

S

G

G

S

C

L

M

F

L

G

G

A

S

R

R

A

G

W

W

G

G

V

V

K

A

P

P

D

D

F

I

M

L

C

C

F

L

A

A

K
|
K

G

G

L

I

S

T

S

A

G

G

Y

Y

V

I

P

P

L

L

G

G

A

A

T

T

V

L

F

F

N

N

S

Q

R

R

V

I

A

A

Q

D

A

A

F

I

E

E

E

N

N

G

Q

Q

D

G

F

F

S

S

G

H

T

M

V

I

M

M

H

H

G

G

Y

Y

T
|
T

Y

Y

A

S

G

G

H

H

P

P

V

T

G

A

C

C

A

A

A

A

A

A

L

L

A

A

S

V

L

L

D

D

I

I

V

V

L

E

N

A

E

E

D

D

L

L

P

P

A

G

N

N

A

A

G

A

K

K

Q

V

G

G

A

A

Y

Q

L

L

L

L

E

E

A

Q

L

L

Q

Q

P

P

F

L

V

V

E

E

R

R

Y

Y

E

A

A

V

V

V

G

G

E

E

V

V

R

R

G

G

K

K

G

G

L

L

M

M

V

I

A

A

I

L

D

D

L

L

V

V

N

S

D

D

K

K

K

R

T

T

R

R

E

Q

S

P

I

L

S

D

P

P

M

A

S

A

G

G

Y

Q

A

P

Y

S

S

R

L

I

A

A

Q

D

A

E

V

A

R

R

Q

R

H

A

G

G

A

V

I

L

V

V

R

R

P

P

V

I

G

G

T

N

K

K

I

I

I

I

M

L

S

S

P

P

P

P

L

L

V

T

I

L

Q

T

R

R

D

D

E

E

I

A

D

G

T

L

I

M

V

V

N

S

A

A

L

L

D

E

A

A

A

A

F

F

active site: Y148 (= Y144), D255 (= D250), K284 (= K279), T321 (= T316)
binding pyridoxal-5'-phosphate: G115 (= G111), S116 (= S112), Y148 (= Y144), H149 (= H145), G150 (= G146), E222 (= E217), D255 (= D250), V257 (= V252), K284 (= K279)

5lhaA Amine transaminase crystal structure from an uncultivated pseudomonas species in the pmp-bound form
58% identity, 98% coverage: 3:440/447 of query aligns to 3:443/447 of 5lhaA

query
sites
5lhaA

Y

Y

E

K

N

N

S

A

-

E

-

K

R

K

F

F

W

W

H

H

P

P

M

M

M

G

H

S

P

S

A

A

A

A

M

P

K

H

Q

R

R

D

K

K

P

T

L

L

R

V

I

I

V

A

R

R

G

G

E

D

G

G

S

N

Y

Y

V

I

F

T

D

D

D

I

A

D

G

G

S

Q

K

R

F

M

V

L

D

D

G

G

V

V

G

G

G

G

L

L

W

W

N

N

V

V

N

N

V

I

G

G

H

H

N

N

R

R

P

A

E

S

V

V

K

K

A

A

A

A

I

I

V

A

K

A

Q

Q

L

L

D

D

Q

E

L

L

E

A

Y

Y

F

Y

Q

Q

L

T

F

F

D

D

G

G

V

I

S

A

N

H

P

P

R

R

A

V

E

F

E

D

L

L

S

A

A

E

R

R

L

L

I

T

D

G

V

M

L

F

A

A

P

Q

E

E

D

R

M

M

Q

A

R

R

V

V

V

L

F

F

S

S

S

S

N

G

G
|
G

S
|
S

D

D

A

A

T

V

E

E

T

T

A

A

L

L

K

K

L

M

A

A

R

R

Q

Q

Y

Y

W

W

K

I

V

A

M

S

G

G

Q

E

K

P

D

G

R

R

T

T

K

R

F

F

I

L

S

S

L

L

K

R

E

N

G

G

Y
|
Y

H
|
H

G
|
G

S

V

H

H

F

M

G

G

S

G

A

T

A

S

V

V

N

G

G

G

N

N

M

G

V

V

F

Y

R

H

R

Y

N

N

Y

H

E

G

P

Q

V

L

M

L

P

A

G

G

C

C

S

H

H

L

V

L

Q

D

N

T

P

P

W

W

V

L

Y

Y

R

R

N

N

P

P

F

W

-

D

T

C

H

R

D

D

P

P

E

Q

Q

A

L

L

A

T

V

A

L

H

C

C

A

I

Q

R

L

Q

L

L

E

E

R

E

E

Q

I

I

E

A

F

L

Q

L

G

G

P

A

D

Q

T

T

V

I

A

A

A

A

F

L

I

I

A

A

E
|
E

P

P

V

V

Q

Q

G

G

A

A

G

G

G

G

V

V

I

I

V

V

P

P

P

P

A

A

S

D

Y

Y

W

W

P

P

M

R

V

L

R

R

Q

E

V

V

C

C

D

D

K

R

Y

H

G

G

V

I

L

L

L

L

I

I

A

A

D
|
D

E

E

V

V

V

V

T

T

G

G

F

F

G

G

R

R

T

S

G

G

S

C

L

M

F

L

G

G

A

S

R

R

A

G

W

W

G

G

V

V

K

A

P

P

D

D

F

I

M

L

C

C

F

L

A

A

K
|
K

G

G

L

I

S

T

S

A

G

G

Y

Y

V

I

P

P

L

L

G

G

A

A

T

T

V

L

F

F

N

N

S

Q

R

R

V

I

A

A

Q

D

A

A

F

I

E

E

E

N

N

G

Q

Q

D

G

F

F

S

S

G

H

T

M

V

I

M

M

H

H

G

G

Y
|
Y

T
|
T

Y

Y

A

S

G

G

H

H

P

P

V

T

G

A

C

C

A

A

A

A

A

A

L

L

A

A

S

V

L

L

D

D

I

I

V

V

L

E

N

A

E

E

D

D

L

L

P

P

A

G

N

N

A

A

G

A

K

K

Q

V

G

G

A

A

Y

Q

L

L

L

L

E

E

A

Q

L

L

Q

Q

P

P

F

L

V

V

E

E

R

R

Y

Y

E

A

A

V

V

V

G

G

E

E

V

V

R

R

G

G

K

K

G

G

L

L

M

M

V

I

A

A

I

L

D

D

L

L

V

V

N

S

D

D

K

K

K

R

T

T

R

R

E

Q

S

P

I

L

S

D

P

P

M

A

S

A

G

G

Y

Q

A

P

Y

S

S

R

L

I

A

A

Q

D

A

E

V

A

R

R

Q

R

H

A

G

G

A

V

I

L

V

V

R

R

P

P

V

I

G

G

T

N

K

K

I

I

I

I

M

L

S

S

P

P

P

P

L

L

V

T

I

L

Q

T

R

R

D

D

E

E

I

A

D

G

T

L

I

M

V

V

N

S

A

A

L

L

D

E

A

A

A

A

F

F

active site: Y146 (= Y144), D253 (= D250), K282 (= K279), T319 (= T316)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G113 (= G111), S114 (= S112), Y146 (= Y144), H147 (= H145), G148 (= G146), E220 (= E217), D253 (= D250), K282 (= K279), Y318 (= Y315), T319 (= T316)

6s54A Transaminase from pseudomonas fluorescens (see paper)
53% identity, 98% coverage: 10:445/447 of query aligns to 18:453/453 of 6s54A

query
sites
6s54A

H

H

P

P

M
|
M

M

Q

H

D

P

P

A

K

A

A

M

L

K

H

Q

E

R

Q

K

R

P

P

L

L

R

I

I

I

V

Q

R

S

G

G

E

K

G

G

S

V

Y

H

V

I

F

T

D

D

D

V

A

D

G

G

S

R

K

R

F

F

V

I

D

D

G

C

V

Q

G

G

G

G

L

L

W

W

N

C

V

V

N

N

V

A

G

G

H

Y

N

G

R

R

P

R

E

E

V

I

K

I

A

D

A

A

I

V

V

T

K

R

Q

Q

L

M

D

E

Q

E

L

L

E

A

Y

Y

F

Y

Q

S

L

L

F

F

D

P

G

G

V

S

S

T

N

N

P

A

R

P

A

A

E

I

E

A

L

L

S

S

A

Q

R

K

L

L

I

T

D

E

V

V

L

A

A

A

P

E

E

E

D

G

M

M

Q

V

R

K

V

A

V

S

F

F

S

G

S

L

N

G

G
|
G

S
|
S

D

D

A

A

T

V

E

E

T

T

A

A

L

L

K

K

L

I

A

A

R

R

Q

Q

Y

Y

W

W

K

K

V

L

M

E

G

G

Q

Q

K

P

D

D

R

K

T

V

K

K

F

F

I

V

S

S

L

L

K

Y

E

N

G

G

Y
|
Y

H
|
H

G
|
G

S

L

H

N

F

F

G

G

S

G

A

M

A

S

V

A

N

C

G

G

N

G

M

N

V

A

F

W

R

K

R

S

N

S

Y

Y

E

E

P

P

V

L

M

M

P

P

G

G

C

F

S

F

H

Q

V

V

Q

E

N

S

P

P

W

H

V

L

Y

Y

R

R

N

N

P

P

F

F

T

T

H

N

D

D

P

P

E

E

Q

E

L

L

A

A

V

E

L

I

C

C

A

A

Q

Q

L

I

L

L

E

E

R

R

E

Q

I

I

E

E

F

M

Q

Q

G

A

P

P

D

G

T

T

V

V

A

A

A

A

F

L

I

I

A

A

E
|
E

P

P

V

I

Q

Q

G

G

A

A

G

G

G

G

V

V

I

I

V

V

P

P

P

P

A

A

S

S

Y

Y

W

W

P

P

M

R

V

L

R

R

Q

Q

V

I

C

C

D

D

K

K

Y

Y

G

D

V

I

L

L

L

L

I

I

A

A

D
|
D

E

E

V
|
V

V
x
I

T

T

G

G

F

L

G

G

R

R

T

S

G

G

S

S

L

L

F

F

G

G

A

S

R

R

A

G

W

W

G

G

V

V

K

K

P

P

D

D

F

I

M

M

C

C

F

L

A

A

K
|
K

G

G

L

I

S

S

S

S

G

G

Y

Y

V

V

P

P

L

L

G

S

A

A

T

T

V

L

F

V

N

N

S

S

R

R

V

V

A

A

Q

R

A

A

F

W

E

E

E

R

N

D

Q

A

D

G

F

F

S

T

G

S

T

V

V

Y

M

M

H

H

G

G

Y

Y

T

T

Y

Y

A

S

G

G

H

H

P

P

V

V

G

S

C

C

A

A

A

A

A

A

L

L

A

A

S

A

L

I

D

D

I

I

V

V

L

L

N

Q

E

E

D

N

L

L

P

A

A

E

N

N

A

A

G

R

K

V

Q

V

G

G

A

D

Y

Y

L

F

L

L

E

E

A

K

L

L

Q

L

P

I

F

L

V

K

E

D

R

K

Y

H

E

R

A

A

V

I

G

G

E

D

V

V

R

R

G

G

K

K

G

G

L

L

M

M

V

L

A

A

I

V

D

E

L

L

V

V

N

K

D

E

K

R

K

A

T

T

R

K

E

E

S

P

I

F

S

G

P

P

M

A

S

D

G

A

Y

Y

A

P

Y

L

S

A

L

I

A

S

Q

E

A

A

V

C

R

V

Q

N

H

N

G

G

A

V

I

M

V

I

R

R

P

T

V

I

G

V

T

N

K

K

I

L

I

I

M

I

S

S

P

P

P

P

L

L

V

T

I

F

Q

T

R

T

D

E

E

H

I

V

D

D

T

E

I

V

V

I

N

E

A

V

L

L

D

D

A

R

A

A

F

F

K

V

E

A

T

N

P

P

F

W

active site: M20 (= M12), Y152 (= Y144), D258 (= D250), K287 (= K279)
binding pyridoxal-5'-phosphate: G119 (= G111), S120 (= S112), Y152 (= Y144), H153 (= H145), G154 (= G146), E225 (= E217), D258 (= D250), V260 (= V252), I261 (≠ V253), K287 (= K279)

6io1B Crystal structure of a novel thermostable (s)-enantioselective omega- transaminase from thermomicrobium roseum (see paper)
44% identity, 97% coverage: 10:443/447 of query aligns to 18:446/448 of 6io1B

query
sites
6io1B

H

H

P

P

M
x
L

M

T

H

N

P

L

A

Y

A

Q

M

L

K

R

Q

R

R

E

K

G

P

P

L

L

R

V

I

L

V

V

R

R

G

G

E

E

G

G

S

V

Y

W

V

V

F

W

D

D

D

A

A

E

G

G

S

K

K

R

F

Y

V

L

D

D

G

G

V

F

G

A

G

G

L

L

W

W

N

N

V

V

N

N

V

I

G

G

H

H

N

G

R

R

P

R

E

E

V

L

K

A

A

E

A

A

I

A

V

R

K

E

Q

Q

L

M

D

E

Q

R

L

V

E

A

Y

F

F

V

Q

P

L

T

F

F

D

F

G

G

V

L

S

A

N

S

P

P

R

P

A

T

E

I

E

E

L

L

S

A

A

A

R

R

L

L

I

A

D

E

V

-

L

L

A

F

P

P

E

G

D

P

M

L

Q

D

R

H

V

F

V

Q

F

F

S

T

S

S

N
x
G

G
|
G

S
x
A

D

E

A

S

T
x
N

E

E

T

T

A

A

L

I

K

K

L

I

A

A

R

R

Q

Y

Y

Y

W

W

K

W

V

L

M

K

G

G

Q

Q

K

P

D

E

R

R

T

V

K

K

F

I

I

L

S

S

L

R

K

R

E

M

G

A

Y
|
Y

H
|
H

G

G

S

I

H

A

F

M

G

G

S

A

A

L

A

S

V

A

N

T

G

G

N

V

M

P

V

A

F

Y

R

W

R

E

N

G

Y

F

E

G

P

P

V

R

M

P

P

P

G

G

C

F

S

I

H

H

V

L

Q

T

N

A

P

P

W

Y

V

K

Y

Y

R

R

N

F

P

G

F

E

T

G

H

L

D

T

P

D

E

E

Q

E

L

F

A

V

V

A

L

R

C

L

A

V

Q

Q

L

E

L

L

E

E

R

E

E

T

I

I

E

E

F

R

Q

E

G

G

P

S

D

E

T

T

V

I

A

A

A

A

F

F

I

I

A

G

E

E

P

P

V

V

Q

Q

G

G

A

A

G

G

G

G

V

V

I

V

V

V

P

P

P

P

A

D

S

G

Y

Y

W

W

P

P

M

A

V

I

R

A

Q

A

V

V

C

L

D

R

K

K

Y

Y

G

G

V

I

L

L

L

L

I

I

A

L

D
|
D

E

E

V
|
V

V
x
I

T

T

G

G

F

F

G

G

R

R

T

T

G

G

S

T

L

L

F

F

G

G

A

M

R

Q

A

Q

W

Y

G

G

V

V

K

Q

P

P

D

D

F

I

M

V

C

T

F

F

A

A

K
|
K

G

G

L

I

S

T

S

S

G

G

Y

Y

V

V

P

P

L

L

G

G

A

G

T

V

V

G

F

V

N

S

S

D

R

E

V

I

A

A

Q

E

A

T

F

L

E

A

E

S

N

A

Q

D

D

R

F

-

S

-

G

-

T

V

V

F

M

M

H

H

G

G

Y

F

T

T

Y

Y

A

S

G

G

H

H

P

P

V

V

G

A

C

C

A

A

A

V

A

A

L

L

A

R

S

N

L

L

D

D

I

I

V

L

L

L

N

A

E

E

D

R

L

L

P

W

A

E

N

N

A

A

G

A

K

A

Q

S

G

G

A

A

Y

Y

L

L

L

L

E

Q

A

E

L

L

Q

K

P

R

F

L

V

E

E

E

R

R

Y

-

E

P

A

Y

V

V

G

G

E

E

V

V

R

R

G

G

K

K

G

G

L

L

M

M

V

L

A

L

I

V

D

E

L

V

V

V

N

R

D

D

K

K

K

A

T

S

R

K

E

E

S

K

I

F

S

P

P

P

M

E

S

F

G

K

Y

L

A

G

Y

P

S

K

L

L

A

E

Q

A

A

A

V

T

R

R

Q

R

H

R

G

G

A

I

I

I

V

V

R

R

P

C

V

T

G

P

T

D

K

G

I

I

I

V

M

M

S

A

P

P

P

P

L

L

V

T

I

I

Q

S

R

R

D

A

E

E

I

C

D

D

T

V

I

L

V

I

N

E

A

G

L

V

D

A

A

A

A

A

F

L

K

S

E

D

T

V

active site: L20 (≠ M12), Y151 (= Y144), D257 (= D250), K286 (= K279)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (≠ N110), G118 (= G111), A119 (≠ S112), N122 (≠ T115), Y151 (= Y144), H152 (= H145), D257 (= D250), V259 (= V252), I260 (≠ V253), K286 (= K279)

5kr6B Directed evolution of transaminases by ancestral reconstruction. Using old proteins for new chemistries
40% identity, 99% coverage: 1:442/447 of query aligns to 10:459/460 of 5kr6B

query
sites
5kr6B

M

L

S

E

Y

M

E

D

N

R

S

A

R

H

F

F

W

F

H

H

P

P

M

S

M

T

H

H

P

L

A

R

A

D

M

H

K

A

Q

S

R

G

K

E

-

L

P

P

L

G

R

R

I

I

V

I

R

T

G

G

-

G

E

K

G

G

S

I

Y

R

V

I

F

Q

D

D

D

S

A

E

G

G

S

R

K

E

F

Y

V

I

D

D

G

A

V

F

G

A

G

G

L

L

W

Y

N

C

V

V

N

N

V

V

G

G

H

Y

N

G

R

R

P

T

E

E

V

I

K

A

A

D

A

A

I

I

V

Y

K

E

Q

Q

L

A

D

K

Q

E

L

L

E

A

Y

Y

F

Y

Q

H

L

T

F

Y

D

V

G

G

V

H

S

S

N

N

P

E

R

P

A

I

E

I

E

E

L

L

S

S

A

E

R

R

L

I

I

I

D

R

V

E

L

W

A

A

P

P

E

A

D

G

M

M

Q

S

R

K

V

V

V

Y

F

Y

S

G

S

M

N
x
S

G
|
G

S
|
S

D

D

A

A

T

N

E

E

T

T

A

Q

L

I

K

K

L

L

A

V

R

W

Q

Y

Y

Y

W

N

K

N

V

V

M

L

G

G

Q

R

K

P

D

Q

R

K

T

K

K

K

F

I

I

I

S

S

L

R

K

Q

E

R

G

G

Y
|
Y

H
|
H

G
|
G

S

S

H

G

F

I

G

M

S

T

A

G

A

S

V

L

N

T

G

G

N

L

M

P

V

A

F

F

R

H

R

N

N

H

Y

F

E

D

P

L

V

P

M

L

P

E

G

P

C

I

S

R

H

H

V

T

Q

T

N

C

P

P

W

H

V

Y

Y

Y

R

R

N

A

P

P

F

A

T

G

H

M

D

S

P

E

E

A

Q

E

L

F

A

S

V

R

L

H

C

C

A

A

Q

D

L

E

L

L

E

E

R

K

E

M

I

I

E

L

F

A

Q

E

G

G

P

P

D

D

T

T

V

V

A

A

A

A

F

F

I

I

A

G

E
|
E

P

P

V

V

Q

M

G

G

A

T

G

G

G

G

V

I

I

V

V

P

P

P

P

P

A

E

S

G

Y

Y

W

W

P

E

M

A

V

I

R

Q

Q

A

V

V

C

L

D

N

K

K

Y

Y

G

D

V

I

L

L

L

L

I

I

A

A

D
|
D

E

E

V
|
V

V

V

T

C

G

G

F

F

G

G

R

R

T

T

G

G

S

S

L

M

F

F

G

G

A

S

R

H

A

H

W

Y

G

G

V

M

K

K

P

P

D

D

F

L

M

I

C

T

F

V

A

A

K
|
K

G

G

L

L

S

T

S

S

G

A

Y

Y

V

A

P

P

L

L

G

S

A

G

T

V

V

I

F

V

N

G

S

E

R

K

V

V

A

W

Q

K

A

V

F

L

E

E

E

Q

N

G

Q

S

D

D

F

Q

S

Y

G

G

T

P

V

I

M

G

H

H

G

G

Y

W

T

T

Y

Y

A

S

G

G

H

H

P

P

V

I

G

C

C

A

A

A

A

A

A

A

L

L

A

A

S

N

L

L

D

D

I

I

V

I

L

E

N

R

E

E

D

N

L

L

P

T

A

G

N

N

A

A

G

A

K

D

Q

T

G

G

A

A

Y

Y

L

F

L

Q

E

Q

A

R

L

M

Q

R

P

E

F

T

V

F

E

G

R

D

Y

H

E

P

A

L

V

V

G

G

E

E

V

V

R

R

G

G

K

V

G

G

L

L

M

M

V

A

A

A

I

L

D

E

L

F

V

V

N

A

D

D

K

K

K

D

T

K

R

R

E

T

S

R

I

F

S

D

P

P

M

S

S

L

G

K

Y

V

A

G

Y

P

S

R

L

V

A

S

Q

A

A

A

V

C

R

L

Q

E

H

D

G

G

A

M

I

I

V

A

R

R

-

A

-

M

P

P

V

H

G

G

T

D

K

I

I

L

I

G

M

F

S

A

P

P

P

P

L

L

V

V

I

I

Q

T

R

R

D

A

E

E

I

V

D

D

T

E

I

I

V

V

N

D

-

I

-

A

-

K

-

Q

A

A

L

V

D

D

A

A

A

V

F

A

K

D

E

E

binding pyridoxal-5'-phosphate: S121 (≠ N110), G122 (= G111), S123 (= S112), Y155 (= Y144), H156 (= H145), G157 (= G146), E228 (= E217), D261 (= D250), V263 (= V252), K290 (= K279)

5kquC Directed evolution of transaminases by ancestral reconstruction. Using old proteins for new chemistries
40% identity, 98% coverage: 4:442/447 of query aligns to 11:453/459 of 5kquC

query
sites
5kquC

E

D

N

R

S

A

R

H

F

F

W

M

H

H

P

P

M

S

M

T

H

H

P

A

A

H

A

D

M

H

K

A

Q

S

R

G

K

A

-

L

P

P

L

G

R

R

I

I

V

I

R

T

G

G

-

A

E

S

G

G

S

I

Y

R

V

I

F

R

D

D

D

H

A

E

G

G

S

R

K

E

F

L

V

I

D

D

G

A

V

F

G

A

G

G

L

L

W

Y

N

C

V

V

N

N

V

I

G

G

H

Y

N

G

R

R

P

T

E

E

V

V

K

A

A

D

A

A

I

I

V

Y

K

K

Q

Q

L

A

D

K

Q

E

L

L

E

A

Y

Y

F

Y

Q

H

L

T

F

Y

D

V

G

G

V

H

S

S

N

T

P

E

R

A

A

I

E

I

E

E

L

L

S

S

A

S

R

R

L

I

I

I

D

R

V

D

L

W

A

A

P

P

E

A

D

G

M

M

Q

K

R

K

V

V

V

Y

F

Y

S

G

S

L

N
x
S

G
|
G

S
|
S

D

D

A

A

T

N

E

E

T

T

A

Q

L

I

K

K

L

L

A

V

R

R

Q

Y

Y

Y

W

N

K

N

V

V

M

L

G

G

Q

R

K

P

D

Q

R

K

T

K

K

K

F

I

I

I

S

S

L

R

K

Q

E

R

G

G

Y
|
Y

H
|
H

G

G

S

S

H

G

F

I

G

M

S

T

A

G

A

S

V

L

N

T

G

G

N

L

M

P

V

S

F

F

R

H

R

Q

N

H

Y

F

E

D

P

L

V

P

M

V

P

E

G

G

C

I

S

K

H

H

V

T

Q

V

N

C

P

P

W

H

V

W

Y

Y

R

K

N

A

P

P

F

A

T

G

H

M

D

D

P

E

E

A

Q

A

L

F

A

V

V

R

L

Y

C

C

A

A

Q

D

L

E

L

L

E

E

R

K

E

L

I

I

E

L

F

A

Q

E

G

G

P

P

D

D

T

T

V

V

A

A

A

A

F

F

I

I

A

G

E

E

P

P

V

V

Q

M

G

G

A

T

G

G

G

G

V

I

I

I

V

V

P

P

P

P

A

K

S

G

Y

Y

W

W

P

E

M

A

V

I

R

Q

Q

A

V

V

C

L

D

N

K

K

Y

Y

G

D

V

V

L

L

L

L

I

I

A

A

D
|
D

E

E

V
|
V

V

V

T

C

G

A

F

F

G

G

R

R

T

L

G

G

S

S

L

K

F

M

G

G

A

S

R

Q

A

R

W

Y

G

G

V

M

K

R

P

P

D

D

F

L

M

I

C

T

F

T

A

A

K
|
K

G

G

L

L

S

T

S

S

G

A

Y

Y

V

A

P

P

L

L

G

S

A

A

T

V

V

I

F

V

N

G

S

E

R

K

V

V

A

W

Q

D

A

V

F

I

E

E

E

K

N

A

Q

S

D

Q

F

K

S

E

G

G

T

A

V

M

M

G

H

H

G

G

Y
x
W

T
|
T

Y

Y

A

S

G

G

H

H

P

P

V

I

G

C

C

A

A

A

A

A

A

A

L

L

A

A

S

N

L

L

D

D

I

I

V

L

L

E

N

R

E

E

D

N

L

L

P

T

A

A

N

N

A

A

G

A

K

D

Q

V

G

G

A

A

Y

Y

L

L

L

N

E

Q

A

R

L

L

Q

R

P

E

F

T

V

F

E

E

R

G

Y

H

E

P

A

L

V

V

G

G

E

E

V

V

R

R

G

G

K

D

G

G

L

M

M

L

V

A

A

A

I

L

D

E

L

F

V

M

N

A

D

D

K

R

K

E

T

A

R

R

E

T

S

P

I

F

S

D

P

P

M

A

S

L

G

K

Y

V

A

G

Y

P

S

K

L

V

A

S

Q

A

A

A

V

C

R

L

Q

E

H

D

G

G

A

M

I

I

V

A

R

R

-

A

-

M

P

P

V

H

G

G

T

D

K

I

I

L

I

G

M

F

S

A

P

P

P

P

L

L

V

V

I

L

Q

T

R

R

D

A

E

E

I

A

D

D

T

E

I

I

V

V

N

G

A

I

L

A

D

K

A

A

A

A

F

V

K

D

E

E

binding pyridoxal-5'-phosphate: S119 (≠ N110), G120 (= G111), S121 (= S112), Y153 (= Y144), H154 (= H145), D259 (= D250), V261 (= V252), K288 (= K279), W324 (≠ Y315), T325 (= T316)

6gwiB The crystal structure of halomonas elongata amino-transferase (see paper)
39% identity, 99% coverage: 2:443/447 of query aligns to 8:445/450 of 6gwiB

query
sites
6gwiB

S

A

Y

L

E

D

N

R

S

A

R

H

F

H

W

L

H

H

P

P

M
x
F

M

T

H

D

P

F

A

K

A

A

M

L

K

G

Q

E

R

E

K

G

P

S

L

R

R

V

I

V

V

T

R

H

G

A

E

E

G

G

S

V

Y

Y

V

I

F

H

D

D

D

S

A

E

G

G

S

N

K

R

F

I

V

L

D

D

G

G

V

M

G

A

G

G

L

L

W

W

N

C

V

V

N

N

V

L

G

G

H

Y

N

G

R

R

P

R

E

E

V

L

K

V

A

E

A

A

I

A

V

T

K

A

Q

Q

L

L

D

E

Q

Q

L

L

E

P

Y

Y

F

Y

Q

N

L

T

F

F

D

F

G

K

V

T

S

T

N

H

P

P

R

P

A

A

E

V

E

R

L

L

S

A

A

E

R

K

L

L

I

C

D

D

V

-

L

L

A

A

P

P

E

A

D

H

M

I

Q

N

R

R

V

V

V

F

F

F

S

T

S

G

N
x
S

G
|
G

S
|
S

D

E

A

A

T

N

E

D

T

T

A

V

L

L

K

R

L

M

A

V

R

R

Q

R

Y

Y

W

W

K

A

V

L

M

K

G

G

Q

Q

K

P

D

D

R

K

T

Q

K

W

F

I

I

I

S

G

L

R

K

E

E

N

G

A

Y
|
Y

H
|
H

G
|
G

S

S

H

T

F

L

G

A

S

G

A

M

A

S

V

L

N

G

G

G

N

M

M

A

V

P

F

M

R

H

R

A

N

Q

Y

G

E

G

P

P

V

C

M

V

P

P

G

G

C

I

S

A

H

H

V

I

Q

R

N

Q

P

P

W

Y

V

W

Y

F

R

G

N

E

P

G

F

R

T

D

H

M

D

S

P

P

E

E

Q

A

L

F

A

G

V

Q

L

T

C

C

A

A

Q

E

L

A

L

L

E

E

R

E

E

K

I

I

E

L

F

E

Q

L

G

G

P

E

D

E

T

K

V

V

A

A

A

A

F

F

I

I

A

A

E
|
E

P

P

V

V

Q

Q

G

G

A

A

G

G

G

G

V

A

I

I

V

M

P

P

P

P

A

E

S

S

Y

Y

W

W

P

P

M

A

V

V

R

K

Q

K

V

V

C

L

D

A

K

K

Y

Y

G

D

V

I

L

L

L

L

I

V

A

A

D
|
D

E

E

V
|
V

V
x
I

T

C

G

G

F

F

G

G

R

R

T

L

G

G

S

E

L

W

F

F

G

G

A

S

R

Q

A

H

W

Y

G

G

V

L

K

E

P

P

D

D

F

L

M

M

C

P

F

I

A

A

K
|
K

G

G

L

L

S

S

S

S

G

G

Y

Y

V

L

P

P

L

I

G

G

A

G

T

V

V

L

F

V

N

G

S

D

R

R

V

V

A

A

Q

E

A

T

F

L

E

I

E

E

N

E

Q

-

D

-

F

-

S

G

G

G

T

E

V

F

M

F

H

H

G

G

Y

F

T

T

Y

Y

A

S

G

G

H

H

P

P

V

T

G

C

C

A

A

A

A

V

A

A

L

L

A

K

S

N

L

L

D

E

I

L

V

L

L

E

N

A

E

E

D

G

L

V

P

V

A

D

N

R

A

V

G

R

K

D

Q

D

-

L

G

G

A

P

Y

Y

L

L

L

A

E

E

A

R

L

W

Q

A

P

S

F

L

V

V

E

D

R

-

Y

H

E

P

A

I

V

V

G

G

E

E

V

A

R

R

G

S

K

L

G

G

L

L

M

M

V

G

A

A

I

L

D

E

L

L

V

V

N

A

D

D

K

K

K

T

T

T

R

G

E

Q

S

R

I

F

S

D

P

K

M

S

S

L

G

G

Y

A

A

G

Y

N

S

L

L

C

A

R

Q

D

A

L

V

C

R

F

Q

A

H

N

G

G

A

L

I

V

V

M

R

R

P

S

V

V

G

G

T

D

K

T

I

M

I

I

M

I

S

S

P

P

P

P

L

L

V

V

I

I

Q

R

R

R

D

E

E

E

I

I

D

D

T

E

I

L

V

V

N

E

A

L

L

A

D

R

A

R

A

A

F

L

K

D

E

E

T

T

active site: F18 (≠ M12), Y149 (= Y144), D255 (= D250), K284 (= K279)
binding pyridoxal-5'-phosphate: S115 (≠ N110), G116 (= G111), S117 (= S112), Y149 (= Y144), H150 (= H145), G151 (= G146), E222 (= E217), D255 (= D250), V257 (= V252), I258 (≠ V253), K284 (= K279)

7qx3A Structure of the transaminase tr2e2 with eos (see paper)
39% identity, 94% coverage: 26:443/447 of query aligns to 4:417/422 of 7qx3A

query
sites
7qx3A

I

I

V

T

R

R

G

A

E

E

G

G

S

C

Y

Y

V

L

F

W

D

D

D

S

A

D

G

G

S

N

K

Q

F

I

V

L

D

D

G

G

V

M

G

A

G

G

L

L

W

W

N

C

V

V

N

N

V

I

G

G

H

Y

N

G

R

R

P

K

E

E

V

L

K

A

A

E

A

V

I

A

V

Y

K

R

Q

Q

L

M

D

Q

Q

E

L

L

E

P

Y

Y

F

Y

Q

N

L

N

F

F

D

F

G

Q

V

C

S

S

N

H

P

P

R

P

A

A

E

I

E

E

L

L

S

S

A

-

R

R

L

L

I

L

D

S

V

E

L

V

A

T

P

P

E

K

D

H

M

M

Q

N

R

H

V

V

V

F

F

F

S

T

S

G

N

S

G

G

S

S

D

D

A

S

T

N

E

D

T

T

A

I

L

L

K

R

L

M

A

V

R

R

Q

Y

Y

Y

W

W

K

K

V

L

M

L

G

G

Q

K

K

P

D

Y

R

K

T

K

K

V

F

V

I

I

S

S

L

R

K

E

E

N

G

A

Y
|
Y

H

H

G

G

S

S

H

T

F

V

G

A

S

G

A

A

A

S

V

L

N

S

G

G

N

M

M

K

V

A

F

M

R

H

R

S

N

H

Y

G

E

D

P

L

V

P

M

I

P

P

G

G

C

I

S

E

H

H

V

I

Q

E

N

Q

P

P

W

Y

V

H

Y

F

R

G

N

R

P

A

F

P

T

D

H

M

D

D

P

P

E

A

Q

E

L

F

A

G

V

R

L

Q

C

A

A

A

Q

Q

L

A

L

L

E

E

R

R

E

K

I

I

E

D

F

E

Q

I

G

G

P

E

D

C

T

N

V

V

A

A

A

A

F

F

I

I

A

A

E

E

P

P

V

I

Q

Q

G

G

A
|
A

G

G

G

G

V

V

I

I

V

I

P

P

P

P

A

D

S

S

Y

Y

W

W

P

P

M

E

V

I

R

K

Q

R

V

I

C

C

D

A

K

E

Y

R

G

D

V

I

L

L

L

L

I

I

A

V

D

D

E

E

V

V

V

I

T

T

G

G

F

F

G

G

R

R

T

L

G

G

S

T

L

W

F

F

G

G

A

S

R

Q

A

Y

W

Y

G

D

V

L

K

Q

P

P

D

D

F

L

M

M

C

P

F

I

A

A

K
|
K

G

G

L

L

S

S

S

S

G

G

Y

Y

V

M

P

P

L

I

G

G

A

G

T

V

V

M

F

V

N

S

S

D

R

R

V

V

A

A

Q

K

A

V

F

V

E

I

E

E

N

E

Q

-

D

-

F

-

S

G

G

G

T

E

V

F

M

F

H

H

G

G

Y

Y

T

T

Y

Y

A

S

G

G

H

H

P

P

V

V

G

A

C

A

A

A

A

V

A

A

L

A

A

E

S

N

L

I

D

R

I

I

V

M

L

R

N

D

E

E

D

G

L

I

P

I

A

E

N

R

A

A

G

G

K

A

Q

E

G

I

A

A

Y

P

L

Y

L

L

E

Q

A

A

L

R

Q

W

P

R

F

E

V

L

E

G

R

E

Y

H

E

P

A

L

V

V

G

G

E

E

V

A

R

R

G

G

K

V

G

G

L

M

M

V

V

A

A

A

I

L

D

E

L

L

V

V

N

K

D

S

K

K

K

Q

T

P

R

L

E

E

S

R

I

F

S

E

P

E

M

-

S

P

G

G

Y

K

A

V

Y

G

S

S

L

L

A

C

Q

R

A

D

V

L

R

S

-

V

Q

K

H

N

G

G

A

L

I

V

V

M

R

R

P

A

V

V

G

G

T

G

K

T

I

M

I

I

M

I

S

S

P

P

P

P

L

L

V

V

I

L

Q

S

R

R

D

E

E

Q

I

V

D

D

T

E

I

L

V

I

N

D

A

K

L

A

D

R

A

R

A

T

F

L

K

D

E

E

T

T

binding 2-azanylethyl hydrogen sulfate: Y121 (= Y144), A199 (= A222), K256 (= K279)

5kr5A Directed evolution of transaminases by ancestral reconstruction. Using old proteins for new chemistries
39% identity, 99% coverage: 1:443/447 of query aligns to 6:452/455 of 5kr5A

query
sites
5kr5A

M

L

S

E

Y

M

E

D

N

R

S

A

R

H

F

F

W

M

H

H

P

P

M

S

M

T

H

H

P

A

A

Y

A

D

M

H

K

A

Q

S

R

G

K

A

-

L

P

P

L

G

R

R

I

I

V

I

R

T

G

G

-

G

E

K

G

G

S

I

Y

R

V

I

F

E

D

D

D

H

A

E

G

G

S

R

K

E

F

Y

V

I

D

D

G

A

V

F

G

A

G

G

L

L

W

Y

N

C

V

V

N

N

V

I

G

G

H

Y

N

G

R

R

P
x
E

E

E

V

V

K

A

A
x
D

A

A

I

I

V

Y

K

E

Q

Q

L

A

D

K

Q

Q

L

L

E

A

Y

Y

F

Y

Q

H

L

T

F

Y

D

V

G

G

V

H

S

S

N

N

P

D

R

P

A

V

E

I

E

E

L

L

S

S

A

S

R

R

L

I

I

I

D

E

V

D

L

W

A

A

P

P

E

A

D

G

M

M

Q

K

R

K

V

V

V

F

F

Y

S

G

S

M

N
x
S

G
|
G

S
|
S

D

D

A

A

T

N

E

E

T

T

A

Q

L

I

K

K

L

L

A

V

R

W

Q

Y

Y

Y

W

N

K

N

V

V

M

L

G

G

Q

R

K

P

D

N

R

K

T

K

K

K

F

I

I

I

S

A

L

R

K

E

E

R

G

S

Y
|
Y

H
|
H

G
|
G

S

S

H

G

F

I

G

V

S

T

A

G

A

S

V

L

N

T

G

G

N

L

M

P

V

S

F

F

R

H

R

Q

N

H

Y

F

E

D

P

L

V

P

M

I

P

D

G

R

C

V

S

K

H

H

V

T

Q

V

N

C

P

P

W

H

V

W

Y

Y

R

N

N

A

P

P

F

P

T

G

H

M

D

S

P

E

E

A

Q

Q

L

F

A

V

V

A

L

Y

C

C

A

V

Q

E

L

E

L

L

E

E

R

K

E

L

I

I

E

A

F

R

Q

E

G

G

P

A

D

D

T

T

V

I

A

A

A

A

F

F

I

I

A

A

E
|
E

P

P

V

V

Q

M

G

G

A

T

G

G

G

G

V

I

I

V

V

P

P

P

P

P

A

Q

S

G

Y

Y

W

W

P

E

M

A

V

I

R

Q

Q

A

V

V

C

L

D

R

K

K

Y

H

G

D

V

I

L

L

L

L

I

I

A

A

D
|
D

E

E

V
|
V

V

V

T

C

G

G

F

F

G

G

R

R

T

L

G

G

S

S

L

K

F

T

G

G

A

S

R

E

A

H

W

Y

G

G

V

I

K

K

P

P

D

D

F

L

M

I

C

T

F

V

A

A

K
|
K

G

G

L

L

S

T

S

S

G

A

Y

Y

V

A

P

P

L

L

G

S

A

A

T

V

V

I

F

V

N

S

S

E

R

K

V

V

A

W

Q

D

A

V

F

I

E

E

E

K

N

G

Q

S

D

R

F

E

S

H

G

G

T

V

V

M

M

G

H

H

G

G

Y
x
W

T
|
T

Y

Y

A

S

G

G

H

H

P

P

V

V

G

C

C

A

A

A

A

A

A

A

L

L

A

A

S

N

L

L

D

D

I

I

V

L

L

E

N

R

E

E

D

N

L

L

P

T

A

G

N

N

A

A

G

A

K

D

Q

V

G

G

A

A

Y

Y

L

L

L

Q

E

Q

A

R

L

L

Q

H

P

E

F

A

V

F

E

G

R

A

Y

H

E

P

A

L

V

V

G

G

E

E

V

V

R

R

G

G

K

V

G

G

L

M

M

L

V

A

A

A

I

L

D

E

L

F

V

M

N

A

D

D

K

K

K

G

T

A

R

R

E

T

S

P

I

F

S

D

P

P

M

A

S

L

G

K

Y

V

A

S

Y

Q

S

K

L

V

A

A

Q

A

A

A

V

A

R

L

Q

E

H
x
D

G

G

A

L

I

I

V

V

R

R

-

A

-

L

P

P

V

H

G

G

T

D

K

I

I

L

I

G

M

F

S

A

P

P

P

P

L

L

V

V

I

T

Q

T

R

R

D

A

E

E

I

V

D

D

T

E

I

I

V

V

N

A

A

I

L

A

D

K

A
x
E

A

A

F

F

K

D

E

E

T

V

binding calcium ion: E66 (≠ P59), D70 (≠ A63), D412 (≠ H405), E447 (≠ A438)
binding pyridoxal-5'-phosphate: S117 (≠ N110), G118 (= G111), S119 (= S112), Y151 (= Y144), H152 (= H145), G153 (= G146), E224 (= E217), D257 (= D250), V259 (= V252), K286 (= K279), W322 (≠ Y315), T323 (= T316)

7q9xAAA Probable aminotransferase
38% identity, 97% coverage: 10:442/447 of query aligns to 16:444/455 of 7q9xAAA

query
sites
7q9xAAA

H

H

P

P

M

F

M

T

H

D

P

T

A

A

A

S

M

L

K

N

Q

Q

R

A

K

G

P

A

L

R

R

V

I

M

V

T

R

R

G

G

E

E

G

G

S

V

Y

Y

V

L

F

W

D

D

D

S

A

E

G

G

S

N

K

K

F

I

V

I

D

D

G

G

V

M

G

A

G

G

L
|
L

W
|
W

N

C

V

V

N

N

V

V

G

G

H

Y

N

G

R

R

P

K

E

D

V

F

K

A

A

E

A

A

I

A

V

R

K

R

Q

Q

L

M

D

E

Q

E

L

L

E

P

Y

F

F

Y

Q

N

L

T

F

F

D

F

G

K

V

T

S

T

N

H

P

P

R

A

A

V

E

V

E

E

L

L

S

S

A

S

R

L

L

L

I

A

D

E

V

V

L

-

A

T

P

P

E

A

D

G

M

F

Q

D

R

R

V

V

V

F

F

Y

S

T

S

N

N
x
S

G
|
G

S
|
S

D

E

A

S

T

V

E

D

T

T

A

M

L

I

K

R

L

M

A

V

R

R

Q

R

Y

Y

W

W

K

D

V

V

M

Q

G

G

Q

K

K

P

D

E

R

K

T

K

K

T

F

L

I

I

S

G

L

R

K

W

E

N

G

G

Y
|
Y

H

H

G
|
G

S

S

H

T

F

I

G

G

S

G

A

A

A

S

V

L

N

G

G

G

N

M

M

K

V

Y

F

M

R

H

R

E

N

Q

Y

G

E

D

P

L

V

P

M

I

P

P

G

G

C

M

S

A

H

H

V

I

Q

E

N

Q

P

P

W

W

V

W

Y

Y

R

K

N

H

P

G

F

K

T

D

H

M

D

T

P

P

E

D

Q

E

L

F

A

G

V

V

L

V

C

A

A

A

Q

R

L

W

L

L

E

E

R

E

E

K

I

I

E

L

F

E

Q

I

G

G

P

A

D

D

T

K

V

V

A

A

A

A

F

F

I

V

A

G

E
|
E

P

P

V

I

Q

Q

G

G

A

A

G

G

G

G

V

V

I

I

V

V

P

P

P

P

A

A

S

T

Y

Y

W

W

P

P

M

E

V

I

R

E

Q

R

V

I

C

C

D

R

K

K

Y

Y

G

D

V

V

L

L

L

L

I

V

A

A

D
|
D

E

E

V
|
V

V
x
I

T

C

G

G

F

F

G

G

R

R

T

T

G

G

S

E

L

W

F

F

G

G

A

H

R

Q

A

H

W

F

G

G

V

F

K

Q

P

P

D

D

F

L

M

F

C

T

F

A

A

A

K
|
K

G

G

L

L

S

S

S

S

G

G

Y

Y

V

L

P

P

L

I

G

G

A

A

T

V

V

F

F

V

N

G

S

K

R

R

V

V

A

A

Q

E

A

G

F

L

E

I

E

A

N

G

Q

G

D

D

F

F

S

N

G

-

T

-

V

-

M

-

H

H

G

G

Y
x
F

T
|
T

Y

Y

A

S

G

G

H

H

P

P

V

V

G

C

C

A

A

A

A

V

A

A

L

H

A

A

S

N

L

V

D

A

I

A

V

L

L

R

N

D

E

E

D

G

L

I

P

V

A

Q

N

R

A

V

G

K

K

D

Q

D

-

I

G

G

A

P

Y

Y

L

M

L

Q

E

K

A

R

L

W

Q

R

P

E

F

T

V

F

E

S

R

R

Y

F

E

E

A

H

V

V

G

D

E

D

V

V

R

R

G

G

K

V

G

G

L

M

M

V

V

Q

A

A

I

F

D

T

L

L

V

V

N

K

D

N

K

K

K

A

T

K

R

R

E

E

S

-

I

L

S

F

P

P

M

D

S

F

G

G

Y

E

A

I

Y

G

S

T

L

L

A

C

Q

R

A

D

V

I

-

F

R

F

Q

R

H

N

G

N

A

L

I

I

V

M

R
|
R

P

A

V

C

G

G

T

D

K

H

I

I

I

V

M

S

S

A

P

P

P

P

L

L

V

V

I

M

Q

T

R

R

D

A

E

E

I

V

D

D

T

E

I

M

V

L

N

A

A

V

L

A

D

E

A

R

A

C

F

L

K

E

E

E

binding (3E)-4-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}-2-oxobut-3-enoic acid: L55 (= L49), W56 (= W50), S115 (≠ N110), G116 (= G111), S117 (= S112), Y149 (= Y144), G151 (= G146), E222 (= E217), D255 (= D250), V257 (= V252), I258 (≠ V253), K284 (= K279), F316 (≠ Y315), T317 (= T316), R412 (= R410)
binding pyridoxal-5'-phosphate: F316 (≠ Y315), T317 (= T316)

4a6tC Crystal structure of the omega transaminase from chromobacterium violaceum in complex with plp (see paper)
38% identity, 97% coverage: 10:442/447 of query aligns to 16:444/455 of 4a6tC

query
sites
4a6tC

H

H

P

P

M
x
F

M

T

H

D

P

T

A

A

A

S

M

L

K

N

Q

Q

R

A

K

G

P

A

L

R

R

V

I

M

V

T

R

R

G

G

E

E

G

G

S

V

Y

Y

V

L

F

W

D

D

D

S

A

E

G

G

S

N

K

K

F

I

V

I

D

D

G

G

V

M

G

A

G

G

L

L

W

W

N

C

V

V

N

N

V

V

G

G

H

Y

N

G

R

R

P

K

E

D

V

F

K

A

A

E

A

A

I

A

V

R

K

R

Q

Q

L

M

D

E

Q

E

L

L

E

P

Y

F

F

Y

Q

N

L

T

F

F

D

F

G

K

V

T

S

T

N

H

P

P

R

A

A

V

E

V

E

E

L

L

S

S

A

S

R

L

L

L

I

A

D

E

V

V

L

-

A

T

P

P

E

A

D

G

M

F

Q

D

R

R

V

V

V

F

F

Y

S

T

S

N

N

S

G
|
G

S
|
S

D

E

A

S

T

V

E

D

T

T

A

M

L

I

K

R

L

M

A

V

R

R

Q

R

Y

Y

W

W

K

D

V

V

M

Q

G

G

Q

K

K

P

D

E

R

K

T

K

K

T

F

L

I

I

S

G

L

R

K

W

E

N

G

G

Y
|
Y

H
|
H

G
|
G

S

S

H

T

F

I

G

G

S

G

A

A