SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_047212727.1 NCBI__GCF_001931675.1:WP_047212727.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9YHT2 Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; Iron-sulfur subunit of complex II; Ip; EC 1.3.5.1 from Gallus gallus (Chicken) (see 2 papers)
31% identity, 22% coverage: 8:95/406 of query aligns to 180:273/290 of Q9YHT2

query
sites
Q9YHT2

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C196 (= C25) binding [4Fe-4S] cluster
C199 (= C28) binding [4Fe-4S] cluster
C202 (= C31) binding [4Fe-4S] cluster
C206 (= C35) binding [3Fe-4S] cluster
W211 (≠ L40) binding a ubiquinone
C253 (= C75) binding [3Fe-4S] cluster
C259 (= C81) binding [3Fe-4S] cluster
C263 (= C85) binding [4Fe-4S] cluster

Sites not aligning to the query:

103 binding [2Fe-2S] cluster
108 binding [2Fe-2S] cluster
111 binding [2Fe-2S] cluster
123 binding [2Fe-2S] cluster

Q56224 NADH-quinone oxidoreductase subunit 9; NADH dehydrogenase I subunit 9; NDH-1 subunit 9; EC 7.1.1.- from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
26% identity, 29% coverage: 22:138/406 of query aligns to 50:182/182 of Q56224

query
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Q56224

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C53 (= C25) binding [4Fe-4S] cluster
C56 (= C28) binding [4Fe-4S] cluster
S57 (≠ G29) binding [4Fe-4S] cluster
C59 (= C31) binding [4Fe-4S] cluster
C63 (= C35) binding [4Fe-4S] cluster
C98 (= C75) binding [4Fe-4S] cluster
I99 (≠ L76) binding [4Fe-4S] cluster
C101 (= C78) binding [4Fe-4S] cluster
C104 (= C81) binding [4Fe-4S] cluster
C108 (= C85) binding [4Fe-4S] cluster

5odqB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with bromoethanesulfonate. (see paper)
23% identity, 54% coverage: 168:388/406 of query aligns to 2:237/291 of 5odqB

query
sites
5odqB

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binding Non-cubane [4Fe-4S]-cluster: G8 (= G174), C9 (= C175), C41 (= C207), C42 (= C208), C78 (≠ A247), G80 (= G249), C81 (= C250), G152 (≠ P309), C153 (= C310), H154 (≠ T311), C193 (= C342), C194 (= C343), C231 (≠ G382), F233 (≠ L384), C234 (= C385)
binding 2-bromanylethanesulfonic acid: G46 (≠ R212), G80 (= G249), H154 (≠ T311), G197 (≠ A346), R201 (≠ S350), F233 (≠ L384), L236 (= L387)

5odhH Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with heterodisulfide for 3.5 minutes (see paper)
23% identity, 54% coverage: 168:388/406 of query aligns to 2:237/291 of 5odhH

query
sites
5odhH

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binding Non-cubane [4Fe-4S]-cluster: G8 (= G174), C9 (= C175), C41 (= C207), C42 (= C208), C78 (≠ A247), G80 (= G249), C81 (= C250), C153 (= C310), H154 (≠ T311), C193 (= C342), C194 (= C343), C231 (≠ G382), F233 (≠ L384), C234 (= C385)
binding 1-thioethanesulfonic acid: A44 (= A210), P45 (≠ I211), G46 (≠ R212), G80 (= G249), H154 (≠ T311), G197 (≠ A346), G198 (= G347)
binding Coenzyme B: R201 (≠ S350), F233 (≠ L384)

Sites not aligning to the query:

binding Coenzyme B: 240

5odhB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with heterodisulfide for 3.5 minutes (see paper)
23% identity, 54% coverage: 168:388/406 of query aligns to 2:237/291 of 5odhB

query
sites
5odhB

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binding Non-cubane [4Fe-4S]-cluster: G8 (= G174), C9 (= C175), I10 (≠ V176), C41 (= C207), C42 (= C208), C78 (≠ A247), G80 (= G249), C81 (= C250), G152 (≠ P309), C153 (= C310), H154 (≠ T311), C193 (= C342), C194 (= C343), C231 (≠ G382), F233 (≠ L384), C234 (= C385)
binding 1-thioethanesulfonic acid: P45 (≠ I211), G46 (≠ R212), G80 (= G249), F233 (≠ L384)

5odcB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus at 2.3 a resolution (see paper)
23% identity, 54% coverage: 168:388/406 of query aligns to 2:237/291 of 5odcB

query
sites
5odcB

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G

S

N

A

V

L

K

T

V

R

R

D

H

L

F

A

A

E

E

I

L

L

I

P

Y

D

N

-

D

-

I

F

G

T

V

D

D

Q

K

L

I

I

A

A

E

L

K

A

V

R

E

R

R

A

P

D

L

A

N

P

I

R

N

V

V

A

G

F

V

H

H

S

Y

P
x
G

C
|
C

T

H

L

F

Q

L

H

K

G

P

Q

T

Q

D

I

V

R

K

-

H

-

L

G

G

K

S

V

A

E

E

-

R

-

P

-

V

-

M

-

L

-

D

A

E

L

I

L

V

R

E

G

A

L

T

G

G

V

A

D

K

V

S

A

V

L

P

P

Y

A

A

D

D

S

K

H

M

L

M

C
|
C

G

G

S

A

A
x
G

G

G

T

G

Y

V

S

R

I

A

L

R

Q

E

P

L

A

E

L

L

S

S

K

L

Q

D

L

M

R

T

D

N

Q

E

K

K

L

I

A

E

C

N

L

M

H

I

A

K

T

A

Q

G

A

A

E

D

E

-

I

-

V

C

S

T

A

V

N

N

I

V

G
x
C

C

P

L
x
F

C
|
C

H

H

L

L

Q

Q

binding Non-cubane [4Fe-4S]-cluster: G8 (= G174), C9 (= C175), I10 (≠ V176), C41 (= C207), C42 (= C208), C78 (≠ A247), N79 (≠ S248), G80 (= G249), C81 (= C250), G152 (≠ P309), C153 (= C310), C193 (= C342), C194 (= C343), G197 (≠ A346), C231 (≠ G382), F233 (≠ L384), C234 (= C385)

6mysB Avian mitochondrial complex ii with atpenin a5 bound, sidechain outside (see paper)
31% identity, 22% coverage: 8:95/406 of query aligns to 135:228/240 of 6mysB

query
sites
6mysB

F

Y

L

L

R

Q

D

S

T

I

P

E

D

D

G

R

E

Q

E

K

A

L

D

D

A

G

I

-

L

L

R

Y

K

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

I

Y

K

R

Q

W

M

M

A

I

E

D

G

S

-

R

-

D

A

Y

P

T

V

E

S

E

R

R

L

L

T

A

Q

Q

A

L

H

Q

-

D

-

P

-

F

-

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

S

R

T

T

C
|
C

P

P

S

K

G

G

V

L

Q

N

Y

P

G

G

R

K

L

A

V

I

binding 3-[(2s,4s,5r)-5,6-dichloro-2,4-dimethyl-1-oxohexyl]-4-hydroxy-5,6-dimethoxy-2(1h)-pyridinone: P162 (= P36), W166 (≠ L40), H209 (≠ L76), I211 (≠ C78)
binding fe3-s4 cluster: C161 (= C35), Y171 (≠ L45), P174 (= P48), C208 (= C75), T210 (= T77), I211 (≠ C78), M212 (≠ R79), N213 (= N80), C214 (= C81)
binding iron/sulfur cluster: C151 (= C25), I152 (≠ V26), L153 (≠ H27), C154 (= C28), A155 (≠ G29), C157 (= C31), A175 (≠ R49), C218 (= C85)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 59, 61, 63, 64, 65, 66, 78

6myrB Avian mitochondrial complex ii with thiapronil bound (see paper)
31% identity, 22% coverage: 8:95/406 of query aligns to 135:228/240 of 6myrB

query
sites
6myrB

F

Y

L

L

R

Q

D

S

T

I

P

E

D

D

G

R

E

Q

E

K

A

L

D

D

A

G

I

-

L

L

R

Y

K

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

I

Y

K

R

Q

W

M

M

A

I

E

D

G

S

-

R

-

D

A

Y

P

T

V

E

S

E

R

R

L

L

T

A

Q

Q

A

L

H

Q

-

D

-

P

-

F

-

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

S

R

T

T

C
|
C

P

P

S

K

G

G

V

L

Q

N

Y

P

G

G

R

K

L

A

V

I

binding fe3-s4 cluster: C161 (= C35), Y171 (≠ L45), P174 (= P48), C208 (= C75), H209 (≠ L76), T210 (= T77), I211 (≠ C78), M212 (≠ R79), N213 (= N80), C214 (= C81)
binding thiapronil: P162 (= P36), W166 (≠ L40), H209 (≠ L76), I211 (≠ C78)
binding iron/sulfur cluster: C151 (= C25), I152 (≠ V26), L153 (≠ H27), C154 (= C28), A155 (≠ G29), C157 (= C31), A175 (≠ R49), C218 (= C85)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 58, 59, 63, 64, 65, 66, 78

6myqB Avian mitochondrial complex ii with ferulenol bound (see paper)
31% identity, 22% coverage: 8:95/406 of query aligns to 134:227/239 of 6myqB

query
sites
6myqB

F

Y

L

L

R

Q

D

S

T

I

P

E

D

D

G

R

E

Q

E

K

A

L

D

D

A

G

I

-

L

L

R

Y

K

E

C
|
C

V
x
I

H
x
L

C
|
C

G

A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P

P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

I

Y

K

R

Q

W

M

M

A

I

E

D

G

S

-

R

-

D

A

Y

P

T

V

E

S

E

R

R

L

L

T

A

Q

Q

A

L

H

Q

-

D

-

P

-

F

-

S

L

L

D

Y

R

R

C
|
C

L

H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

S

R

T

T

C
|
C

P
|
P

S

K

G

G

V

L

Q

N

Y

P

G

G

R

K

L

A

V

I

binding 4-oxidanyl-3-[(2~{E},6~{E})-3,7,11-trimethyldodeca-2,6,10-trienyl]chromen-2-one: W165 (≠ L40), I210 (≠ C78)
binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), T209 (= T77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), C156 (= C31), A174 (≠ R49), C217 (= C85), P218 (= P86)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 77

6mypB Avian mitochondrial complex ii with ttfa (thenoyltrifluoroacetone) bound (see paper)
31% identity, 22% coverage: 8:95/406 of query aligns to 135:228/240 of 6mypB

query
sites
6mypB

F

Y

L

L

R

Q

D

S

T

I

P

E

D

D

G

R

E

Q

E

K

A

L

D

D

A

G

I

-

L

L

R

Y

K

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L

Y

D

L

G

G

P

P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

I

Y

K

R

Q

W

M

M

A

I

E

D

G

S

-

R

-

D

A

Y

P

T

V

E

S

E

R

R

L

L

T

A

Q

Q

A

L

H

Q

-

D

-

P

-

F

-

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C

I

R
x
M

N
|
N

C
|
C

E

T

S

R

T

T

C
|
C

P

P

S

K

G

G

V

L

Q

N

Y

P

G

G

R

K

L

A

V

I

binding fe3-s4 cluster: C161 (= C35), C208 (= C75), H209 (≠ L76), T210 (= T77), M212 (≠ R79), N213 (= N80), C214 (= C81)
binding iron/sulfur cluster: C151 (= C25), I152 (≠ V26), L153 (≠ H27), C154 (= C28), A155 (≠ G29), C157 (= C31), A175 (≠ R49), C218 (= C85)
binding 4,4,4-trifluoro-1-thien-2-ylbutane-1,3-dione: P162 (= P36), W166 (≠ L40), H209 (≠ L76)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 58, 59, 61, 63, 64, 65, 66, 78

6myoB Avian mitochondrial complex ii with flutolanyl bound (see paper)
31% identity, 22% coverage: 8:95/406 of query aligns to 135:228/240 of 6myoB

query
sites
6myoB

F

Y

L

L

R

Q

D

S

T

I

P

E

D

D

G

R

E

Q

E

K

A

L

D

D

A

G

I

-

L

L

R

Y

K

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P

P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

I

Y

K

R

Q

W

M

M

A

I

E

D

G

S

-

R

-

D

A

Y

P

T

V

E

S

E

R

R

L

L

T

A

Q

Q

A

L

H

Q

-

D

-

P

-

F

-

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

S

R

T

T

C
|
C

P

P

S

K

G

G

V

L

Q

N

Y

P

G

G

R

K

L

A

V

I

binding fe3-s4 cluster: C161 (= C35), Y171 (≠ L45), P174 (= P48), C208 (= C75), T210 (= T77), I211 (≠ C78), M212 (≠ R79), N213 (= N80), C214 (= C81)
binding N-[3-(1-methylethoxy)phenyl]-2-(trifluoromethyl)benzamide: W166 (≠ L40), H209 (≠ L76), I211 (≠ C78)
binding iron/sulfur cluster: C151 (= C25), I152 (≠ V26), L153 (≠ H27), C154 (= C28), A155 (≠ G29), C157 (= C31), A175 (≠ R49), C218 (= C85)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 58, 59, 61, 63, 64, 65, 66, 78

2fbwB Avian respiratory complex ii with carboxin bound (see paper)
31% identity, 22% coverage: 8:95/406 of query aligns to 135:228/240 of 2fbwB

query
sites
2fbwB

F

Y

L

L

R

Q

D

S

T

I

P

E

D

D

G

R

E

Q

E

K

A

L

D

D

A

G

I

-

L

L

R

Y

K

E

C
|
C

V
x
I

H
x
L

C
|
C

G

A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

I

Y

K

R

Q

W

M

M

A

I

E

D

G

S

-

R

-

D

A

Y

P

T

V

E

S

E

R

R

L

L

T

A

Q

Q

A

L

H

Q

-

D

-

P

-

F

-

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

S

R

T

T

C
|
C

P
|
P

S

K

G

G

V

L

Q

N

Y

P

G

G

R

K

L

A

V

I

binding 2-methyl-n-phenyl-5,6-dihydro-1,4-oxathiine-3-carboxamide: P162 (= P36), W166 (≠ L40), H209 (≠ L76)
binding fe3-s4 cluster: C161 (= C35), Y171 (≠ L45), P174 (= P48), C208 (= C75), T210 (= T77), I211 (≠ C78), M212 (≠ R79), N213 (= N80), C214 (= C81)
binding iron/sulfur cluster: C151 (= C25), I152 (≠ V26), L153 (≠ H27), C154 (= C28), C157 (= C31), A175 (≠ R49), C218 (= C85), P219 (= P86)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 58, 59, 61, 63, 64, 65, 66, 78

1yq3B Avian respiratory complex ii with oxaloacetate and ubiquinone (see paper)
31% identity, 22% coverage: 8:95/406 of query aligns to 137:230/242 of 1yq3B

query
sites
1yq3B

F

Y

L

L

R

Q

D

S

T

I

P

E

D

D

G

R

E

Q

E

K

A

L

D

D

A

G

I

-

L

L

R

Y

K

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

I

Y

K

R

Q

W

M

M

A

I

E

D

G

S

-

R

-

D

A

Y

P

T

V

E

S

E

R

R

L

L

T

A

Q

Q

A

L

H

Q

-

D

-

P

-

F

-

S

L

L

D

Y

R

R

C
|
C

L

H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

S

R

T

T

C
|
C

P
|
P

S

K

G

G

V

L

Q

N

Y

P

G

G

R

K

L

A

V

I

binding fe3-s4 cluster: C163 (= C35), Y173 (≠ L45), P176 (= P48), C210 (= C75), T212 (= T77), I213 (≠ C78), M214 (≠ R79), N215 (= N80), C216 (= C81)
binding iron/sulfur cluster: C153 (= C25), I154 (≠ V26), L155 (≠ H27), C156 (= C28), A157 (≠ G29), C159 (= C31), A177 (≠ R49), C220 (= C85), P221 (= P86)
binding Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer: P164 (= P36), W168 (≠ L40), I213 (≠ C78)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 60, 61, 63, 65, 66, 67, 68, 80

Q007T0 Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; Iron-sulfur subunit of complex II; Ip; EC 1.3.5.1 from Sus scrofa (Pig) (see paper)
29% identity, 22% coverage: 8:95/406 of query aligns to 170:263/280 of Q007T0

query
sites
Q007T0

F

Y

L

L

R

Q

D

S

T

I

P

E

D

E

G

R

E

E

E

K

A

L

D

D

A

G

I

-

L

L

R

Y

K

E

C
|
C

V

I

H

L

C
|
C

G

A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P

P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L

Y

D

L

G

G

P

P

R

A

G

V

R

L

I

M

Y

Q

L

A

I

Y

K

R

Q

W

M

M

A

I

E

D

G

S

-

R

-

D

A

F

P

T

V

E

S

E

R

R

L

L

T

A

Q

K

A

L

H

Q

-

D

-

P

-

F

-

S

L

L

D

Y

R

R

C
|
C

L

H

T

T

C

I

R

M

N

N

C
|
C

E

T

S

G

T

T

C
|
C

P

P

S

K

G

G

V

L

Q

N

Y

P

G

G

R

K

L

A

V

I

C186 (= C25) binding [4Fe-4S] cluster
C189 (= C28) binding [4Fe-4S] cluster
C192 (= C31) binding [4Fe-4S] cluster
C196 (= C35) binding [3Fe-4S] cluster
W201 (≠ L40) binding a ubiquinone
C243 (= C75) binding [3Fe-4S] cluster
C249 (= C81) binding [3Fe-4S] cluster
C253 (= C85) binding [4Fe-4S] cluster

Sites not aligning to the query:

93 binding [2Fe-2S] cluster
98 binding [2Fe-2S] cluster
101 binding [2Fe-2S] cluster
113 binding [2Fe-2S] cluster

3sfeB Crystal structure of porcine mitochondrial respiratory complex ii bound with oxaloacetate and thiabendazole (see paper)
29% identity, 22% coverage: 8:95/406 of query aligns to 135:228/240 of 3sfeB

query
sites
3sfeB

F

Y

L

L

R

Q

D

S

T

I

P

E

D

E

G

R

E

E

E

K

A

L

D

D

A

G

I

-

L

L

R

Y

K

E

C
|
C

V
x
I

H
x
L

C
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C

G
x
A

F

C

C
|
C

T

S

A

T

T

S

C
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C

P

P

T

S

Y

Y

Q

W

L

W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

I

Y

K

R

Q

W

M

M

A

I

E

D

G

S

-

R

-

D

A

F

P

T

V

E

S

E

R

R

L

L

T

A

Q

K

A

L

H

Q

-

D

-

P

-

F

-

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

S

G

T

T

C
|
C

P
|
P

S
x
K

G

G

V
x
L

Q

N

Y

P

G

G

R

K

L

A

V

I

binding fe3-s4 cluster: C161 (= C35), Y171 (≠ L45), P174 (= P48), C208 (= C75), T210 (= T77), I211 (≠ C78), M212 (≠ R79), N213 (= N80), C214 (= C81)
binding iron/sulfur cluster: C151 (= C25), I152 (≠ V26), L153 (≠ H27), C154 (= C28), A155 (≠ G29), C157 (= C31), A175 (≠ R49), C218 (= C85), P219 (= P86), K220 (≠ S87), L222 (≠ V89)
binding 2-(1,3-thiazol-4-yl)-1h-benzimidazole: H209 (≠ L76), I211 (≠ C78)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 58, 59, 61, 63, 64, 65, 66, 78

3sfdB Crystal structure of porcine mitochondrial respiratory complex ii bound with oxaloacetate and pentachlorophenol (see paper)
29% identity, 22% coverage: 8:95/406 of query aligns to 134:227/239 of 3sfdB

query
sites
3sfdB

F

Y

L

L

R

Q

D

S

T

I

P

E

D

E

G

R

E

E

E

K

A

L

D

D

A

G

I

-

L

L

R

Y

K

E

C
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C

V
x
I

H
x
L

C
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C

G
x
A

F

C

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C

T

S

A

T

T

S

C
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C

P
|
P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

R
x
A

G

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R

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I

M

Y

Q

L

A

I

Y

K

R

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W

M

M

A

I

E

D

G

S

-

R

-

D

A

F

P

T

V

E

S

E

R

R

L

L

T

A

Q

K

A

L

H

Q

-

D

-

P

-

F

-

S

L

L

D

Y

R

R

C
|
C

L

H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

S

G

T

T

C
|
C

P
|
P

S
x
K

G

G

V

L

Q

N

Y

P

G

G

R

K

L

A

V

I

binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), T209 (= T77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding pentachlorophenol: P161 (= P36), W165 (≠ L40), I210 (≠ C78)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), A174 (≠ R49), C217 (= C85), P218 (= P86), K219 (≠ S87)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 64, 65, 77

3aegB Crystal structure of porcine heart mitochondrial complex ii bound with n-biphenyl-3-yl-2-iodo-benzamide
29% identity, 22% coverage: 8:95/406 of query aligns to 134:227/239 of 3aegB

query
sites
3aegB

F

Y

L

L

R

Q

D

S

T

I

P

E

D

E

G

R

E

E

E

K

A

L

D

D

A

G

I

-

L

L

R

Y

K

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P

P

T
x
S

Y

Y

Q
x
W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

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|
P

R
x
A

G

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I

M

Y

Q

L

A

I

Y

K

R

Q

W

M

M

A

I

E

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G

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-

R

-

D

A

F

P

T

V

E

S

E

R

R

L

L

T

A

Q

K

A

L

H

Q

-

D

-

P

-

F

-

S

L

L

D

Y

R

R

C
|
C

L
x
H

T

T

C
x
I

R
x
M

N

N

C
|
C

E

T

S

G

T

T

C
|
C

P

P

S

K

G

G

V

L

Q

N

Y

P

G

G

R

K

L

A

V
x
I

binding N-biphenyl-3-yl-2-iodobenzamide: S162 (≠ T37), W164 (≠ Q39), W165 (≠ L40), H208 (≠ L76)
binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), I210 (≠ C78), M211 (≠ R79), C213 (= C81), I227 (≠ V95)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), A174 (≠ R49), C217 (= C85)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 75, 77

3aeeB Crystal structure of porcine heart mitochondrial complex ii bound with atpenin a5
29% identity, 22% coverage: 8:95/406 of query aligns to 134:227/239 of 3aeeB

query
sites
3aeeB

F

Y

L

L

R

Q

D

S

T

I

P

E

D

E

G

R

E

E

E

K

A

L

D

D

A

G

I

-

L

L

R

Y

K

E

C
|
C

V

I

H
x
L

C
|
C

G

A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P

P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

I

Y

K

R

Q

W

M

M

A

I

E

D

G

S

-

R

-

D

A

F

P

T

V

E

S

E

R

R

L

L

T

A

Q

K

A

L

H

Q

-

D

-

P

-

F

-

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

S

G

T

T

C
|
C

P
|
P

S

K

G

G

V
x
L

Q

N

Y

P

G

G

R

K

L

A

V

I

binding 3-[(2s,4s,5r)-5,6-dichloro-2,4-dimethyl-1-oxohexyl]-4-hydroxy-5,6-dimethoxy-2(1h)-pyridinone: W165 (≠ L40), H208 (≠ L76), I210 (≠ C78)
binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), T209 (= T77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding iron/sulfur cluster: C150 (= C25), L152 (≠ H27), C153 (= C28), C156 (= C31), A174 (≠ R49), C217 (= C85), P218 (= P86), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 77

3aedB Crystal structure of porcine heart mitochondrial complex ii bound with 2-iodo-n-phenyl-benzamide
29% identity, 22% coverage: 8:95/406 of query aligns to 134:227/239 of 3aedB

query
sites
3aedB

F

Y

L

L

R

Q

D

S

T

I

P

E

D

E

G

R

E

E

E

K

A

L

D

D

A

G

I

-

L

L

R

Y

K

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

R

A

G

V

R

L

I

M

Y

Q

L

A

I

Y

K

R

Q

W

M

M

A

I

E

D

G

S

-

R

-

D

A

F

P

T

V

E

S

E

R

R

L

L

T

A

Q

K

A

L

H

Q

-

D

-

P

-

F

-

S

L

L

D

Y

R

R

C
|
C

L
x
H

T

T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

S

G

T

T

C
|
C

P

P

S

K

G

G

V
x
L

Q

N

Y

P

G

G

R

K

L

A

V

I

binding 2-iodo-N-phenylbenzamide: P161 (= P36), W165 (≠ L40), H208 (≠ L76), I210 (≠ C78)
binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), C217 (= C85), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 77

3aecB Crystal structure of porcine heart mitochondrial complex ii bound with 2-iodo-n-(1-methylethyl)-benzamid
29% identity, 22% coverage: 8:95/406 of query aligns to 134:227/239 of 3aecB

query
sites
3aecB

F

Y

L

L

R

Q

D

S

T

I

P

E

D

E

G

R

E

E

E

K

A

L

D

D

A

G

I

-

L

L

R

Y

K

E

C
|
C

V
x
I

H
x
L

C
|
C

G

A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

I

Y

K

R

Q

W

M

M

A

I

E

D

G

S

-

R

-

D

A

F

P

T

V

E

S

E

R

R

L

L

T

A

Q

K

A

L

H

Q

-

D

-

P

-

F

-

S

L

L

D

Y

R

R

C
|
C

L
x
H

T

T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

S

G

T

T

C
|
C

P
|
P

S

K

G

G

V
x
L

Q

N

Y

P

G

G

R

K

L

A

V

I

binding 2-iodo-N-(1-methylethyl)benzamide: P161 (= P36), W165 (≠ L40), H208 (≠ L76)
binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), C156 (= C31), A174 (≠ R49), C217 (= C85), P218 (= P86), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 62, 63, 65, 75, 77

Query Sequence

>WP_047212727.1 NCBI__GCF_001931675.1:WP_047212727.1
MQTNLADFLRDTPDGEEADAILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQMAE
GAPVSRLTQAHLDRCLTCRNCESTCPSGVQYGRLVDIGRRYVDRQVQRSLGERAMRYVLA
NVVPNRKLFGALFEVGRRVRLALPAALRHKIPPRHNVGAWPTRTHARKMLMLAGCVQPAM
MPSVNTATARVLDALGIETVVASEAGCCGAIRLHLGQEADGLDDVRRNIDAWWPHVESGA
EAIVMNASGCGATVKEYGHLLRNDPAYASKAQRISALTRDLAEILPDFTDQLIALARRAD
APRVAFHSPCTLQHGQQIRGKVEALLRGLGVDVALPADSHLCCGSAGTYSILQPALSKQL
RDQKLACLHATQAEEIVSANIGCLCHLQSGTSTPVRHWVELIAQRL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory