SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_047213363.1 NCBI__GCF_001931675.1:WP_047213363.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5i1fA Crystal structure of utp-glucose-1-phosphate uridylyltransferase from burkholderia vietnamiensis in complex with uridine-5'-diphosphate- glucose
77% identity, 96% coverage: 1:284/296 of query aligns to 2:285/290 of 5i1fA

query
sites
5i1fA

M

M

L

L

K

K

V

V

T

T

K

K

A

A

V

V

F

F

P
|
P

V

V

A
|
A

G
|
G

L

L

G

G

T

T

R

R

F

F

L

L

P

P

A

A

T

T

K

K

A

A

S

S

P

P

K
|
K

E
|
E

M

M

L

L

P

P

V

V

D

D

K

K

P

P

L

L

I

I

Q

Q

Y

Y

A

A

V

V

E

E

A

A

A

I

A

E

A

A

G

G

I

I

T

T

E

E

M

M

I

I

F

F

V

V

T

T

G

G

R

R

N

S

K

K

R

R

A

A

I

I

E

E

D

D

H

H

F

F

D

D

K

K

A

S

Y

Y

E

E

L

I

E

E

T

A

E

E

L

L

E

E

A

A

N

R

D

G

K

K

Q

E

T

K

L

L

N

S

T

L

V

V

R

R

S

S

I

I

K

K

P

P

P

S

Q

H

M

V

A

D

C

C

V

F

Y

Y

V

V

R

R

Q
|
Q

P

A

E

E

T
x
A

L
|
L

G
|
G

L
|
L

G

G

H

H

A
|
A

V

V

L

L

C

C

A

A

E

E

T

K

L

L

V

V

G

G

D

D

N

N

P

P

F

F

A

A

V

V

I

I

L

L

A

A

D

D

D
|
D

L

L

I

L

A

D

G

G

E

P

P

T

P

P

A

V

L

L

S

R

Q

Q

M

M

L

I

E

D

A

V

F

F

D

D

H

H

Y

Y

H

H

S

A

S

S

V

V

L

I

G

G

V

V

E

E

I

I

P

A

R

P

E

A

R

D

T

S

R

K

A

S

Y
|
Y

G
|
G

V

V

I

I

D

D

G

G

K

K

A

R

W

W

D

E

D

D

S

D

I

L

I

F

K

K

L

L

S

S

G

G

I

I

V

V

E
|
E

K
|
K

P

P

A

E

P

P

D

A

D

Q

A

A

P

P

S

S

N

N

L

F

G

G

V
|
V

V

V

G

G

R

R

Y

Y

V

V

L

L

M

K

P

P

T

K

I

I

F

F

R

K

H

H

L

L

R

R

R

G

L

L

K

K

P

P

G

G

A

A

G

G

E

E

L

L

Q

Q

L

L

T

T

D

D

A

A

I

I

Q

Q

T

S

L

L

Q

T

E

D

E

E

Q

Q

V

V

L

L

A

A

Y

Y

R

R

Y

Y

R

D

G

G

T

T

R

R

Y

F

D

D

C

C

G

G

S

S

K

K

V

L

G

G

Y

Y

L

L

K

K

A

A

T

T

V

V

E

E

Y

F

A

A

L

L

R

R

H

H

P

P

E

E

V

V

R

A

E

A

E

D

F

F

T

E

E

R

Y

Y

I

L

binding uridine-5'-diphosphate-glucose: P11 (= P10), A13 (= A12), G14 (= G13), K28 (= K27), E29 (= E28), Q106 (= Q105), A109 (≠ T108), L110 (= L109), G111 (= G110), L112 (= L111), A115 (= A114), D135 (= D134), Y172 (= Y171), G173 (= G172), E192 (= E191), K193 (= K192), V205 (= V204)

5ve7A Crystal structure of utp-glucose-1-phosphate uridylyltransferase from burkholderia ambifaria in complex with utp
77% identity, 96% coverage: 2:285/296 of query aligns to 1:280/282 of 5ve7A

query
sites
5ve7A

L

L

K

K

V

V

T

N

K

K

A

A

V

V

F

F

P
|
P

V
|
V

A
|
A

G
|
G

L

L

G
|
G

T
|
T

R
|
R

F

F

L

L

P

P

A

A

T

T

K

K

A

A

S

S

P

P

K
|
K

E
|
E

M

M

L

L

P

P

V

V

D

D

K

K

P

P

L

L

I

I

Q

Q

Y

Y

A

A

V

V

E

E

A

A

A

I

A

A

G

G

I

I

T

T

E

E

M

M

I

I

F

F

V

V

T

T

G

G

R

R

N

S

K

K

R

R

A

A

I

I

E

E

D

D

H

H

F

F

D

D

K

K

A

S

Y

Y

E

E

L

V

E

E

T

A

E

E

L

L

E

E

A

A

N

R

D

G

K

K

Q

A

T

K

L

L

N

E

T

L

V

V

R

R

S

S

I

I

K

K

P

P

P

S

Q

H

M

V

A

D

C

C

V

F

Y

Y

V

V

R

R

Q
|
Q

P

P

E

E

T
x
A

L

L

G
|
G

L

L

G

G

H

H

A
|
A

V

V

L

L

C

C

A

A

E

E

T

K

L

L

V

V

G

A

D

D

N

N

P

P

F

F

A

A

V

V

I

I

L

L

A

A

D

D

L

L

I

L

A

D

G

G

E

N

P

P

A

V

L

M

S

K

Q

Q

M

M

L

V

E

D

A

V

F

F

D

D

H

H

Y

Y

H

H

S

S

V

V

L

I

G

G

V

V

E

E

I

I

P

P

R

P

E

S

R

E

T

T

R

K

A

S

Y

Y

G

G

V

I

I

V

D

D

G

G

K

K

A

E

W

W

D

E

S

S

I

I

I

V

K

K

L

M

S

S

G

A

I

I

V

V

E

E

K

K

P

P

A

A

P

P

D

E

D

V

A

A

P

P

S

S

N

N

L

L

G

G

V

V

G

G

R

R

Y

Y

V

I

L

L

M

K

P

P

T

R

I

I

F

F

R

E

H

H

L

L

R

R

R

A

L

L

K

K

P

P

G

-

A

-

G

-

E

E

L

L

Q

Q

L

L

T

T

D

D

A

A

I

I

Q

Q

T

A

L

L

Q

A

E

D

E

E

Q

Q

V

V

L

L

A

A

Y

Y

R

K

Y

Y

R

Q

G

G

T

T

R

R

Y

Y

D

D

C

C

G

G

S

S

K

K

V

L

G

G

Y

Y

L

L

K

K

A

A

T

T

V

V

E

E

Y

F

A

A

L

L

R

R

H

H

P

P

E

E

V

V

R

G

E

T

E

E

F

F

T

D

E

A

Y

Y

I

L

R

R

binding uridine 5'-triphosphate: P9 (= P10), V10 (= V11), A11 (= A12), G12 (= G13), G14 (= G15), T15 (= T16), R16 (= R17), K26 (= K27), E27 (= E28), Q104 (= Q105), A107 (≠ T108), G109 (= G110), A113 (= A114)

2ux8G Crystal structure of sphingomonas elodea atcc 31461 glucose-1- phosphate uridylyltransferase in complex with glucose-1-phosphate. (see paper)
54% identity, 92% coverage: 6:276/296 of query aligns to 7:275/288 of 2ux8G

query
sites
2ux8G

K

K

A

A

V

V

F

F

P

P

V

V

A

A

G

G

L

L

G

G

T

T

R

R

F

F

L

L

P

P

A

A

T

T

K

K

A

A

S

M

P

P

K

K

E

E

M

M

L

L

P

P

V

V

D

D

K

R

P

P

L

L

I

I

Q

Q

Y

Y

A

A

V

V

E

D

E

E

A

A

A

V

A

E

A

A

G

G

I

I

T

E

E

Q

M

M

I

I

F

F

V

V

T

T

G

G

R

R

N

G

K

K

R

S

A

A

I

L

E

E

D

D

H

H

F

F

D

D

K

I

A

A

Y

Y

E

E

L

L

E

E

T

A

E

T

L

M

E

A

A

A

N

R

D

G

K

K

Q

S

T

L

L

D

L

V

N

L

T

D

V

G

R

T

S

R

I

L

K

K

P

P

P

G

Q

N

M

I

A

A

C

-

V

-

Y

Y

V

V

R

R

Q

Q

P

Q

E

E

T

P

L

M

G

G

L
|
L

G

G

H

H

A

A

V

V

L

W

C

C

A

A

E

R

T

D

L

I

V

V

G

G

D

D

N

E

P

P

F

F

A

A

V

V

I

L

L

L

A

P

D

D

L

F

I

M

A

F

G

G

E

Q

P

P

P

G

A

C

L

L

S

K

Q

Q

M

M

L

V

E

D

A

A

F

Y

D

N

H

K

Y

V

H

G

S

G

S

N

V

L

L

I

G

C

V

A

E

E

I

V

P

P

R

D

E

D

R

Q

T

T

R

H

A

R

Y
|
Y

G
|
G

V

I

I

I

D

T

G

P

K

G

A

T

W

Q

D

D

D

G

S

V

I

L

I

T

K

E

L

V

S

K

G

G

I

L

V

V

E
|
E

K

K

P

P

A

A

P

P

D

G

D

T

A

A

P

P

S

S

N

N

L

L

G

S

V
|
V

V

I

G

G

R

R

Y

Y

V

I

L

L

M

Q

P

P

T

E

I

V

F

M

R

R

H

I

L

L

R

E

R

N

L

Q

K

G

P

K

G

G

A

A

G

G

E

E

L

I

Q

Q

L

L

T
|
T

D

D

A

A

I

M

Q

Q

T

R

L

M

L

I

Q

G

E

D

E

Q

Q

P

V

F

L

H

A

G

Y

V

R

T

Y

F

R

Q

G

G

T

T

R

R

Y

Y

D

D

C

C

G

G

S

D

K

K

V

A

G

G

Y

F

L

I

K

Q

A

A

T

N

V

L

E

A

Y

V

A

A

L

L

R

S

H

R

P

P

E

D

V

L

binding 1-O-phosphono-alpha-D-glucopyranose: L110 (= L111), Y170 (= Y171), G171 (= G172), E190 (= E191), V203 (= V204), T231 (= T232)

8f73E Crystal structure of pseudomonas aeruginosa udp-glucose phosphorylase in complex with udp-glucose (see paper)
51% identity, 89% coverage: 4:266/296 of query aligns to 8:271/281 of 8f73E

query
sites
8f73E

V

I

T

K

K

K

A

C

V

L

F

F

P
|
P

V

A

A
|
A

G
|
G

L

Y

G

G

T

T

R

R

F

F

L

L

P

P

A

A

T

T

K

K

A

A

S

M

P

P

K
|
K

E
|
E

M

M

L

L

P

P

V

V

D

N

K

K

P

P

L

L

I

I

Q

Q

Y

Y

A

A

V

V

E

E

A

A

A

L

A

E

A

A

G

G

I

L

T

S

E

E

M

I

I

G

F

I

V

V

T

T

G

G

R

R

N

G

K

K

R

R

A

S

I

L

E

E

D

D

H

H

F

F

D

D

K

I

A

S

Y

Y

E

E

L

L

E

E

T

H

E

Q

L

I

E

R

A

N

N

T

D

D

K

K

Q

E

T

K

L

Y

L

L

N

V

T

G

V

I

R

R

S

R

I

L

K

I

P

D

P

-

Q

E

M

C

A

T

C

F

V

A

Y

Y

V

T

R

R

Q
|
Q

P

V

E

E

T

M

L

K

G
|
G

L
|
L

G

G

H

H

A
|
A

V

I

L

L

C

T

A

G

E

R

T

P

L

L

V

I

G

G

D

D

N

E

P

P

F

F

A

A

V

V

I

V

L
|
L

A

A

D

D

D
|
D

L

L

-

C

-

L

-

N

I

L

A

E

G

G

E

D

P

S

P

-

A

V

L

L

S

K

Q

Q

M

M

L

V

E

K

A

L

F

Y

D

N

H

Q

Y

F

H

R

S

C

S

S

V

I

L

V

G

A

V

I

E

Q

E

E

I

V

P

P

R

P

E

E

R

E

T

T

R

N

A

K

Y

Y

G

G

V

V

I

I

D

A

G

G

K

E

A

M

W

I

D

R

D

D

S

D

I

I

I

F

K

R

L

V

S

N

G

T

I

M

V

V

E
|
E

K
|
K

P

P

A

K

P

P

D

E

A

A

P

P

S

S

N

N

L

L

G

A

V
x
I

V

I

G

G

R

R

Y

Y

V

I

L

L

M

T

P

P

T

D

I

I

F

F

R

D

H

L

L

I

R

E

R

Q

L

T

K

E

P

P

G

G

A

K

G

G

E

E

L

I

Q

Q

L

I

T

T

D

D

A

A

I

L

Q

M

T

K

L

Q

L

A

Q

Q

E

D

E

G

Q

C

V

V

L

L

A

A

Y

Y

R

K

Y

F

R

K

G

G

T

K

R

R

Y

F

D

D

C

C

G

G

S

S

K

A

V

E

G

G

Y

Y

L

I

K

E

A

A

T

T

binding uridine-5'-diphosphate-glucose: P14 (= P10), A16 (= A12), G17 (= G13), K31 (= K27), E32 (= E28), Q108 (= Q105), G113 (= G110), L114 (= L111), A117 (= A114), L134 (= L131), D137 (= D134), E196 (= E191), K197 (= K192), I209 (≠ V204)

3jukA The crystal structure of udp-glucose pyrophosphorylase complexed with udp-glucose (see paper)
48% identity, 90% coverage: 4:269/296 of query aligns to 2:260/265 of 3jukA

query
sites
3jukA

V

I

T

K

K

K

A

C

V

L

F

F

P

P

V

A

A
|
A

G
|
G

L

Y

G

G

T

T

R

R

F

F

L

L

P

P

A

I

T

T

K

K

A

T

S

I

P

P

K

K

E
|
E

M

M

L

L

P

P

V

I

V

V

D

D

K

K

P

P

L

L

I

I

Q

Q

Y

Y

A

A

V

V

E

E

A

A

A

M

A

E

A

A

G

G

I

C

T

E

E

V

M

M

I

A

F

I

V

V

T

T

G

G

R

R

N

N

K

K

R

R

A

S

I

L

E

E

D

D

H

Y

F

F

D

D

K

T

A

S

Y

Y

E

-

L

-

E

-

T

-

E

-

L

-

E

-

A

-

N

T

D

N

K

K

Q

E

T

N

L

A

L

L

N

K

T

S

V

I

R

R

S

N

I

I

K

-

P

-

P

I

Q

E

M

K

A

C

C

C

V

F

-

S

Y

Y

V

V

R

R

Q
|
Q

P

K

E

Q

T
x
M

L

K

G
|
G

L
|
L

G

G

H

H

A
|
A

V

I

L

L

C

T

A

G

E

E

T

A

L

L

V

I

G

G

D

N

N

E

P

P

F

F

A

A

V

V

I

I

L

L

A

A

D

D

D
|
D

L

L

I

C

A

I

G

S

E

H

-

D

-

H

P

P

P

S

A

V

L

L

S

K

Q

Q

M

M

L

T

E

S

A

L

F

Y

D

Q

H

K

Y

Y

H

Q

S

C

S

S

V

I

L

V

G

A

V

I

E

E

I

V

P

A

R

L

E

E

R

E

T

V

R

S

A

K

Y
|
Y

G
|
G

V

V

I

I

D

R

G

G

K

E

A

W

W

L

D

E

S

G

I

V

I

Y

K

E

L

I

S

K

G

D

I

M

V

V

E
|
E

K
|
K

P

P

A

N

P

Q

D

E

D

D

A

A

P

P

S

S

N

N

L

L

G

A

V
|
V

V

I

G

G

R

R

Y

Y

V

I

L

L

M

T

P

P

T

D

I

I

F

F

R

E

H

I

L

L

R

S

R

E

L

T

K

K

P

P

G

G

A

K

G

N

E

E

L

I

Q

Q

L

I

T

T

D

D

A

A

I

L

Q

R

T

T

L

Q

L

A

Q

K

E

R

E

K

Q

R

V

I

L

I

A

A

Y

Y

R

Q

Y

F

R

K

G

G

T

K

R

R

Y

Y

D

D

C

C

G

G

S

S

K

V

V

E

G

G

Y

Y

L

I

K

E

A

A

T

S

V

N

E

A

Y

Y

binding uridine-5'-diphosphate-glucose: A10 (= A12), G11 (= G13), E26 (= E28), Q94 (= Q105), M97 (≠ T108), G99 (= G110), L100 (= L111), A103 (= A114), D123 (= D134), Y162 (= Y171), G163 (= G172), E182 (= E191), K183 (= K192), V195 (= V204)

3jukD The crystal structure of udp-glucose pyrophosphorylase complexed with udp-glucose (see paper)
48% identity, 90% coverage: 4:269/296 of query aligns to 2:260/264 of 3jukD

query
sites
3jukD

V

I

T

K

K

K

A

C

V

L

F

F

P
|
P

V

A

A
|
A

G
|
G

L

Y

G

G

T
|
T

R
|
R

F

F

L

L

P

P

A

I

T

T

K

K

A

T

S

I

P

P

K

K

E
|
E

M

M

L

L

P

P

V

I

V

V

D

D

K

K

P

P

L

L

I

I

Q

Q

Y

Y

A

A

V

V

E

E

A

A

A

M

A

E

A

A

G

G

I

C

T

E

E

V

M

M

I

A

F

I

V

V

T

T

G

G

R

R

N

N

K

K

R

R

A

S

I

L

E

E

D

D

H

Y

F

F

D

D

K

T

A

S

Y

Y

E

-

L

-

E

-

T

-

E

-

L

-

E

-

A

-

N

T

D

N

K

K

Q

E

T

N

L

A

L

L

N

K

T

S

V

I

R

R

S

N

I

I

K

-

P

-

P

I

Q

E

M

K

A

C

C

C

V

F

-

S

Y

Y

V

V

R

R

Q
|
Q

P

K

E

Q

T
x
M

L

K

G
|
G

L
|
L

G

G

H

H

A
|
A

V

I

L

L

C

T

A

G

E

E

T

A

L

L

V

I

G

G

D

N

N

E

P

P

F

F

A

A

V

V

I

I

L
|
L

A

A

D

D

D
|
D

L

L

I

C

A

I

G

S

E

H

-

D

-

H

P

P

P

S

A

V

L

L

S

K

Q

Q

M

M

L

T

E

S

A

L

F

Y

D

Q

H

K

Y

Y

H

Q

S

C

S

S

V

I

L

V

G

A

V

I

E

E

I

V

P

A

R

L

E

E

R

E

T

V

R

S

A

K

Y
|
Y

G
|
G

V

V

I

I

D

R

G

G

K

E

A

W

W

L

D

E

S

G

I

V

I

Y

K

E

L

I

S

K

G

D

I

M

V

V

E
|
E

K
|
K

P

P

A

N

P

Q

D

E

D

D

A

A

P

P

S

S

N

N

L

L

G

A

V
|
V

V

I

G

G

R

R

Y

Y

V

I

L

L

M

T

P

P

T

D

I

I

F

F

R

E

H

I

L

L

R

S

R

E

L

T

K

K

P

P

G

G

A

K

G

N

E

E

L

I

Q

Q

L

I

T

T

D

D

A

A

I

L

Q

R

T

T

L

Q

L

A

Q

K

E

R

E

K

Q

R

V

I

L

I

A

A

Y

Y

R

Q

Y

F

R

K

G

G

T

K

R

R

Y

Y

D

D

C

C

G

G

S

S

K

V

V

E

G

G

Y

Y

L

I

K

E

A

A

T

S

V

N

E

A

Y

Y

binding magnesium ion: T14 (= T16), R15 (= R17)
binding uridine-5'-diphosphate-glucose: P8 (= P10), A10 (= A12), G11 (= G13), E26 (= E28), Q94 (= Q105), M97 (≠ T108), G99 (= G110), L100 (= L111), A103 (= A114), L120 (= L131), D123 (= D134), Y162 (= Y171), G163 (= G172), E182 (= E191), K183 (= K192), V195 (= V204)

6knlA Uridine and triphosphate-bound ugpase from acinetobacter baumannii
47% identity, 96% coverage: 4:287/296 of query aligns to 2:289/290 of 6knlA

query
sites
6knlA

V

I

T

K

K

K

A

A

V

V

F

L

P
|
P

V

V

A

A

G
|
G

L

L

G
|
G

T
|
T

R
|
R

F

F

L

L

P

P

A

A

T

S

K

K

A

S

S

I

P

P

K
|
K

E

E

M

M

L

V

P

T

V

V

D

D

K

R

P

P

L

A

I

I

Q

E

Y

Y

A

V

V

V

E

R

E

E

A

A

A

V

A

E

A

A

G

G

I

I

T

E

E

Q

M

I

I

I

F

L

V

V

T

T

G

H

R

S

N

S

K

K

R

A

A

S

I

I

E

E

D

N

H

Y

F

F

D

D

K

R

A

N

Y

F

E

E

L

L

E

E

T

T

E

T

L

L

E

E

A

Q

N
x
K

D

K

K
|
K

Q

F

T

D

L

L

N

A

T

E

V

I

R

T

S

Q

I

I

K

V

P

P

P

E

Q

H

M

V

A

S

C

V

V

I

Y

S

V

V

R

R

Q
|
Q

P

P

E

Q

T
x
P

L

L

G
|
G

L

L

G

G

H

H

A

A

V

V

L

L

C

C

A

A

E

K

T

S

L

V

V

V

G

G

D

E

N

D

P

D

F

F

A

A

V

V

I

L

L

L

A
x
P

D
|
D

D

V

L

L

I

V

-

K

-

D

-

G

A

S

G

G

E

Q

P

N

P

D

A

-

L

L

S

S

Q

R

M

M

L

I

E

S

A

R

F

Y

D

N

H

S

Y

S

H

Q

S

A

V

Q

L

I

G

M

V

V

E

E

E

A

I

V

P

P

R

D

E

H

R

L

T

V

R

D

A

Q

Y

Y

G

G

V

I

I

V

D

D

-

V

-

A

G

Q

K

S

A

P

W

N

D

E

D

G

S

E

I

S

I

I

K

A

L

M

S

Q

G

G

I

I

V

V

E

E

K

K

P

P

A

P

P

V

D

G

D

A

A

A

P

P

S

S

N

N

L

L

G

S

V

V

G

G

R

R

Y

Y

V

V

L

L

M

P

P

A

T

K

I

I

F

M

R

Q

H

L

L

L

R

E

R

N

L

T

K

P

P

K

G

G

A

A

G

G

G

N

E

E

L

I

Q

Q

L

L

T

T

D

D

A

A

I

I

Q

A

T

M

L

L

L

Q

Q

D

E

T

E

D

Q

T

V

V

L

E

A

A

Y

Y

R

R

Y

M

R

Q

G

G

T

Q

R

T

Y

F

D

D

C

C

G

G

S

S

K

K

V

L

G

G

Y

Y

L

L

K

K

A

A

T

V

V

L

E

H

Y

Y

A

G

L

L

R

E

H

H

P

P

E

K

V

L

R

G

E

M

E

E

F

F

T

K

E

Q

Y

L

I

I

R

L

Q

E

I

L

binding triphosphate: G13 (= G15), T14 (= T16), R15 (= R17), K79 (≠ N81), K81 (= K83)
binding uridine: P8 (= P10), G11 (= G13), K25 (= K27), Q103 (= Q105), P106 (≠ T108), G108 (= G110), P130 (≠ A132), D131 (= D133)

6k8dA Udp-glucose pyrophosphorylase with upg from acinetobacter baumanii
47% identity, 96% coverage: 4:287/296 of query aligns to 2:289/290 of 6k8dA

query
sites
6k8dA

V

I

T

K

K

K

A

A

V

V

F

L

P
|
P

V

V

A
|
A

G
|
G

L

L

G

G

T

T

R

R

F

F

L

L

P

P

A

A

T

S

K

K

A

S

S

I

P

P

K

K

E

E

M

M

L

V

P

T

V

V

D

D

K

R

P

P

L

A

I

I

Q

E

Y

Y

A

V

V

V

E

R

E

E

A

A

A

V

A

E

A

A

G

G

I

I

T

E

E

Q

M

I

I

I

F

L

V

V

T

T

G

H

R

S

N

S

K

K

R

A

A

S

I

I

E

E

D

N

H

Y

F

F

D

D

K

R

A

N

Y

F

E

E

L

L

E

E

T

T

E

T

L

L

E

E

A

Q

N

K

D

K

K

K

Q

F

T

D

L

L

N

A

T

E

V

I

R

T

S

Q

I

I

K

V

P

P

P

E

Q

H

M

V

A

S

C

V

V

I

Y

S

V

V

R

R

Q
|
Q

P

P

E

Q

T
x
P

L

L

G
|
G

L
|
L

G

G

H

H

A
|
A

V

V

L

L

C

C

A

A

E

K

T

S

L

V

V

V

G

G

D

E

N

D

P

D

F

F

A

A

V

V

I

L

L
|
L

A

P

D
|
D

D

V

L

L

I

V

-

K

-

D

-

G

A

S

G

G

E

Q

P

N

P

D

A

-

L

L

S

S

Q

R

M

M

L

I

E

S

A

R

F

Y

D

N

H

S

Y

S

H

Q

S

A

V

Q

L

I

G

M

V

V

E

E

E

A

I

V

P

P

R

D

E

H

R

L

T

V

R

D

A

Q

Y

Y

G

G

V

I

I

V

D

D

-

V

-

A

G

Q

K

S

A

P

W

N

D

E

D

G

S

E

I

S

I

I

K

A

L

M

S

Q

G

G

I

I

V

V

E
|
E

K

K

P

P

A

P

P

V

D

G

D

A

A

A

P

P

S

S

N

N

L

L

G

S

V
|
V

V

V

G

G

R

R

Y

Y

V

V

L

L

M

P

P

A

T

K

I

I

F

M

R

Q

H

L

L

L

R

E

R

N

L

T

K

P

P

K

G

G

A

A

G

G

G

N

E

E

L

I

Q

Q

L

L

T

T

D

D

A

A

I

I

Q

A

T

M

L

L

L

Q

Q

D

E

T

E

D

Q

T

V

V

L

E

A

A

Y

Y

R

R

Y

M

R

Q

G

G

T

Q

R

T

Y

F

D

D

C

C

G

G

S

S

K

K

V

L

G

G

Y

Y

L

L

K

K

A

A

T

V

V

L

E

H

Y

Y

A

G

L

L

R

E

H

H

P

P

E

K

V

L

R

G

E

M

E

E

F

F

T

K

E

Q

Y

L

I

I

R

L

Q

E

I

L

binding uridine-5'-diphosphate-glucose: P8 (= P10), A10 (= A12), G11 (= G13), Q103 (= Q105), P106 (≠ T108), G108 (= G110), L109 (= L111), A112 (= A114), L129 (= L131), D131 (= D133), E193 (= E191), V206 (= V204)

6ikzB Udp-glucose pyrophosphorylase from acinetobacter baumanii
46% identity, 96% coverage: 4:287/296 of query aligns to 2:284/285 of 6ikzB

query
sites
6ikzB

V

I

T

K

K

K

A

A

V

V

F

L

P
|
P

V
|
V

A
|
A

G
|
G

L
|
L

G
|
G

T
|
T

R
|
R

F

F

L

L

P

P

A

A

T

S

K

K

A

S

S

I

P

P

K
|
K

E

E

M

M

L

V

P

T

V

V

D

D

K

R

P

P

L

A

I

I

Q

E

Y

Y

A

V

V

V

E

R

E

E

A

A

A

V

A

E

A

A

G

G

I

I

T

E

E

Q

M

I

I

I

F

L

V

V

T

T

G

H

R

S

N

S

K

K

R

A

A

S

I

I

E

E

D

N

H

Y

F

F

D

D

K

R

A

N

Y

F

E

E

L

L

E

E

T

T

E

T

L

L

E

E

A

Q

N

K

D

K

K

K

Q

F

T

D

L

L

N

A

T

E

V

I

R

T

S

Q

I

I

K

V

P

P

P

E

Q

H

M

V

A

S

C

V

V

I

Y

S

V

V

R

R

Q
|
Q

P

P

E

Q

T
x
P

L

L

G
|
G

L

L

G

G

H

H

A

A

V

V

L

L

C

C

A

A

E

K

T

S

L

V

V

V

G

G

D

E

N

D

P

D

F

F

A

A

V

V

I

L

L

L

A

P

D
|
D

D

V

L

L

I

V

-

K

-

D

-

G

A

S

G

G

E

Q

P

N

P

D

A

-

L

L

S

S

Q

R

M

M

L

I

E

S

A

R

F

Y

D

N

H

S

Y

S

H

Q

S

A

V

Q

L

I

G

M

V

V

E

E

E

A

I

V

P

P

R

D

E

H

R

L

T

V

R

D

A

Q

Y

Y

G

G

V

I

I

V

D

D

-

V

-

A

G

Q

K

S

A

P

W

N

D

E

D

G

S

E

I

S

I

I

K

A

L

M

S

Q

G

G

I

I

V

V

E

E

K

K

P

P

A

P

P

V

D

G

D

A

A

A

P

P

S

S

N

N

L

L

G

S

V

V

G

G

R

R

Y

Y

V

V

L

L

M

P

P

A

T

K

I

I

F

M

R

Q

H

L

L

L

R

E

R

N

L

T

K

P

P

-

G

-

A

-

G

-

E

E

L

I

Q

Q

L

L

T

T

D

D

A

A

I

I

Q

A

T

M

L

L

L

Q

Q

D

E

T

E

D

Q

T

V

V

L

E

A

A

Y

Y

R

R

Y

M

R

Q

G

G

T

Q

R

T

Y

F

D

D

C

C

G

G

S

S

K

K

V

L

G

G

Y

Y

L

L

K

K

A

A

T

V

V

L

E

H

Y

Y

A

G

L

L

R

E

H

H

P

P

E

K

V

L

R

G

E

M

E

E

F

F

T

K

E

Q

Y

L

I

I

R

L

Q

E

I

L

binding uridine 5'-triphosphate: P8 (= P10), V9 (= V11), A10 (= A12), G11 (= G13), L12 (= L14), G13 (= G15), T14 (= T16), R15 (= R17), K25 (= K27), Q103 (= Q105), P106 (≠ T108), G108 (= G110), D131 (= D133)

2ux8A Crystal structure of sphingomonas elodea atcc 31461 glucose-1- phosphate uridylyltransferase in complex with glucose-1-phosphate. (see paper)
46% identity, 92% coverage: 6:276/296 of query aligns to 7:242/255 of 2ux8A

query
sites
2ux8A

K

K

A

A

V

V

F

F

P

P

V

V

A

A

G

G

L

L

G

G

T

T

R

R

F

F

L

L

P

P

A

A

T

T

K

K

A

A

S

M

P

P

K

K

E

E

M

M

L

L

P

P

V

V

D

D

K

R

P

P

L

L

I

I

Q

Q

Y

Y

A

A

V

V

E

D

E

E

A

A

A

V

A

E

A

A

G

G

I

I

T

E

E

Q

M

M

I

I

F

F

V

V

T

T

G

G

R

R

N

G

K

K

R

S

A

A

I

L

E

E

D

D

H

H

F

F

D

D

K

I

A

A

Y

Y

E

E

L

L

E

E

T

A

E

T

L

M

E

A

A

A

N

R

D

G

K

K

Q

S

T

L

L

D

L

V

N

L

T

D

V

G

R

T

S

R

I

L

K

K

P

P

P

G

Q

N

M

I

A

A

C

-

V

-

Y

Y

V

V

R

R

Q

Q

P

Q

E

E

T

P

L

M

G

G

L

L

G

G

H

H

A

A

V

V

L

W

C

C

A

A

E

R

T

D

L

I

V

V

G

G

D

D

N

E

P

P

F

F

A

A

V

V

I

L

L
|
L

A

P

D

D

L

F

I

M

A

F

G

G

E

Q

P

P

P

G

A

C

L

L

S

K

Q

Q

M

M

L

V

E

D

A

A

F

Y

D

N

H

K

Y

V

H

G

S

G

S

N

V

L

L

I

G

C

V

A

E

E

E

I

I

I

P

-

R

-

E

-

R

-

T

-

R

-

A

T

Y

P

G

G

V

T

I

Q

D

D

G

G

K

V

A

L

W

T

D

E

D

-

S

-

I

-

K

-

L

-

S

-

G

-

I

-

V

-

E

-

K

-

P

-

A

-

P

-

D

-

A

-

P

-

S

V

N

N

L

L

G

S

V
|
V

V

I

G

G

R

R

Y
|
Y

V

I

L

L

M

Q

P

P

T

E

I

V

F

M

R

R

H

I

L

L

R

E

R

N

L

Q

K

G

P

-

G

-

A

-

G

-

E

-

L

-

Q

-

L

L

T

T

D

D

A

A

I

M

Q

Q

T

R

L

M

L

I

Q

G

E

D

E

Q

Q

P

V

F

L

H

A

G

Y

V

R

T

Y

F

R

Q

G

G

T

T

R

R

Y

Y

D

D

C

C

G

G

S

D

K

K

V

A

G

G

Y

F

L

I

K

Q

A

A

T

N

V

L

E

A

Y

V

A

A

L

L

R

S

H

R

P

P

E

D

V

L

binding 1-O-phosphono-alpha-D-glucopyranose: L130 (= L131), V178 (= V204), Y182 (= Y208)

8b6dA Crystal structure of udp-glucose pyrophosphorylase from thermocrispum agreste dsm 44070 in complex with udp
44% identity, 97% coverage: 5:290/296 of query aligns to 3:291/291 of 8b6dA

query
sites
8b6dA

T

T

K

T

A

A

V

I

F

V

P
|
P

V

A

A
|
A

G
|
G

L
|
L

G
|
G

T
|
T

R
|
R

F

F

L

L

P

P

A

T

T

T

K

K

A

S

S

V

P

P

K
|
K

E

E

M

L

L

L

P

P

V

V

D

D

K

T

P

P

L

A

I

I

Q

E

Y

L

A

V

V

A

E

D

E

E

A

A

A

R

A

Q

A

A

G

G

I

A

T

E

E

R

M

L

I

V

F

I

V

V

T

T

G

S

R

P

N

A

K

K

R

Q

A

S

I

I

E

A

D

A

H

Y

F

F

D

R

K

P

A

A

Y

P

E

E

L

L

E

E

T

R

E

S

L

L

E

E

A

E

N

K

D

G

K

K

Q

T

T

G

L

Q

L

L

N

A

T

K

V

I

R

R

S

-

I

-

K

R

P

A

P

P

Q

E

M

L

A

L

C

E

V

V

Y

E

V

V

R

A

-

I

Q
|
Q

P

E

E

Q

T
x
A

L

L

G
|
G

L

L

G

G

H

H

A
|
A

V

V

L

A

C

C

A

A

E

E

T

P

L

N

V

L

G

G

-

P

-

E

D

D

N

D

P

V

F

V

A

A

V

V

I

L

L

L

A

P

D

D

L

L

I

V

A

L

G

P

E

H

P

-

P

G

A

I

L

L

S

E

Q

R

M

M

L

A

E

K

A

V

F

R

D

A

H

E

Y

H

H

G

S

G

S

S

V

V

L

L

G

C

V

A

E

F

E

D

I

I

P

P

R

K

E

E

R

E

T

I

R

S

A

A

Y

Y

G

G

V

V

I

F

D

D

G

V

K

S

A

D

W

T

D

D

S

A

I

D

I

V

K

K

-

R

L

V

S

H

G

G

I

M

V

V

E

E

K

K

P

P

A

P

P

A

D

E

D

Q

A

A

P

P

S

S

N

T

L

F

G

A

V

A

G

G

R

R

Y

Y

V

L

L

L

M

D

P

R

T

A

I

I

F

F

R

D

H

A

L

L

R

R

L

I

K

E

P

P

G

G

A

A

G

G

E

E

L

L

Q

Q

L

L

T

T

D

D

A

A

I

V

Q

A

T

L

L

L

L

I

Q

Q

E

E

E

G

Q

H

-

P

V

V

L

H

A

V

Y

V

R

V

Y

H

R

R

G

G

T

D

R

R

Y

H

D

D

C

L

G

G

S

N

K

P

V

G

G

G

Y

F

L

L

K

R

A

A

T

A

V

V

E

D

Y

F

A

A

L

L

R

Q

H

D

P

P

E

D

V

Y

R

G

E

P

E

E

F

L

T

R

E

A

Y

W

I

L

R

T

-

D

Q

R

I

I

A

A

L

R

P

P

binding uridine-5'-diphosphate: P8 (= P10), A10 (= A12), G11 (= G13), L12 (= L14), G13 (= G15), T14 (= T16), R15 (= R17), K25 (= K27), Q102 (= Q105), A105 (≠ T108), G107 (= G110), A111 (= A114)

2pa4B Crystal structure of udp-glucose pyrophosphorylase from corynebacteria glutamicum in complex with magnesium and udp-glucose (see paper)
42% identity, 96% coverage: 4:287/296 of query aligns to 3:289/299 of 2pa4B

query
sites
2pa4B

V

V

T

K

K

T

A

V

V

V

F

V

P
|
P

V
x
A

A
|
A

G
|
G

L

L

G

G

T

T

R

R

F

F

L

L

P

P

A

A

T

T

K

K

A

T

S

V

P

P

K

K

E
|
E

M

L

L

L

P

P

V

V

D

D

K

T

P

P

L

G

I

I

Q

E

Y

L

A

I

V

A

E

A

E

E

A

A

A

E

A

L

G

G

I

A

T

T

E

R

M

L

I

A

F

I

V

I

T

T

G

A

R

P

N

N

K

K

R

A

A

G

I

V

E

L

D

A

H

H

F

F

D

E

K

R

A

S

Y

S

E

E

L

L

E

E

T

E

E

T

L

L

E

M

A

E

N

R

D

G

K

K

Q

T

T

D

L

Q

L

V

N

E

T

I

V

I

R

R

S

R

I

-

K

A

P

A

P

D

Q

L

M

I

A

K

C

A

V

V

Y

P

V

V

R

T

Q
|
Q

P

D

E

K

T
x
P

L

L

G
|
G

L
|
L

G

G

H

H

A

A

V

V

L

G

C

L

A

A

E

E

T

S

L

V

V

L

G

D

D

D

N

D

P

E

-

D

-

V

F

V

A

A

V

V

I

M

L
|
L

A
x
P

D

D

D
|
D

L

L

I

V

A

L

G

-

E

P

P

T

P

G

A

V

L

M

S

E

Q

R

M

M

L

A

E

Q

A

V

F

R

D

A

H

E

Y

F

H

G

S

G

S

S

V

V

L

L

G

C

V

A

E

V

E

E

I

V

P

S

R

E

E

A

R

D

T

V

R

S

A

K

Y
|
Y

G
|
G

V

I

-

F

-

E

I

I

D

E

G

A

K

D

A

T

W

K

D

D

D

S

S

D

I

V

I

K

K

K

L

V

S

K

G

G

I

M

V

V

E
|
E

K
|
K

P

P

A

A

P

I

D

E

D

D

A

A

P

P

S

S

N

R

L

L

G

A

V
x
A

V

T

G

G

R

R

Y

Y

V

L

L

L

M

D

P

R

T

K

I

I

F

F

R

D

H

A

L

L

R

R

L

I

K

T

P

P

G

G

A

A

G

G

E

E

L

L

Q

Q

L

L

T

T

D

D

A

A

I

I

Q

D

T

L

L

L

L

I

Q

D

E

E

E

G

Q

H

-

P

V

V

L

H

A

I

Y

V

R

I

Y

H

R

Q

G

G

T

K

R

R

Y

H

D

D

C

L

G

G

S

N

K

P

V

G

G

G

Y

Y

L

I

K

P

A

A

T

C

V

V

E

D

Y

F

A

G

L

L

R

S

H

H

P

P

E

V

V

Y

R

G

E

A

E

Q

F

L

T

K

E

D

Y

A

I

I

R

K

Q

Q

I

I

binding uridine-5'-diphosphate-glucose: P9 (= P10), A10 (≠ V11), A11 (= A12), G12 (= G13), E27 (= E28), Q103 (= Q105), P106 (≠ T108), G108 (= G110), L109 (= L111), L131 (= L131), P132 (≠ A132), D134 (= D134), Y170 (= Y171), G171 (= G172), E192 (= E191), K193 (= K192), A205 (≠ V204)

8b68A Crystal structure of udp-glucose pyrophosphorylase from thermocrispum agreste dsm 44070 in complex with udp-glucose
42% identity, 97% coverage: 5:290/296 of query aligns to 3:286/286 of 8b68A

query
sites
8b68A

T

T

K

T

A

A

V

I

F

V

P
|
P

V

A

A
|
A

G
|
G

L

L

G

G

T

T

R

R

F

F

L

L

P

P

A

T

T

T

K

K

A

S

S

V

P

P

K

K

E

E

M

L

L

L

P

P

V

V

D

D

K

T

P

P

L

A

I

I

Q

E

Y

L

A

V

V

A

E

D

E

E

A

A

A

R

A

Q

A

A

G

G

I

A

T

E

E

R

M

L

I

V

F

I

V

V

T

T

G

S

R

P

N

A

K

K

R

Q

A

S

I

I

E

A

D

A

H

Y

F

F

D

R

K

P

A

A

Y

P

E

E

L

L

E

E

T

R

E

S

L

L

E

E

A

E

N

K

D

G

K

K

Q

T

T

G

L

Q

L

L

N

A

T

K

V

I

R

R

S

-

I

-

K

R

P

A

P

P

Q

E

M

L

A

L

C

E

V

V

Y

E

V

V

R

A

-

I

Q
|
Q

P

E

E

Q

T
x
A

L

L

G
|
G

L

L

G

G

H

H

A
|
A

V

V

L

A

C

C

A

A

E

E

T

P

L

N

V

L

G

G

-

P

-

E

D

D

N

D

P

V

F

V

A

A

V

V

I

L

L
|
L

A
x
P

D

D

D
|
D

L

L

I

V

A

L

G

P

E

H

P

-

P

G

A

I

L

L

S

E

Q

R

M

M

L

A

E

K

A

V

F

R

D

A

H

E

Y

H

H

G

S

G

S

S

V

V

L

L

G

C

V

A

E

F

E

D

I

I

P

P

R

K

E

E

R

E

T

I

R

S

A

A

Y

Y

G

G

V

V

I

F

D

D

G

V

K

S

A

D

W

T

D

D

S

A

I

D

I

V

K

K

-

R

L

V

S

H

G

G

I

M

V

V

E

E

K

K

P

P

A

P

P

A

D

E

D

Q

A

A

P

P

S

S

N

T

L

F

G

A

V
x
A

V

A

G
|
G

R

R

Y

Y

V

L

L

L

M

D

P

R

T

A

I

I

F

F

R

D

H

A

L

L

R

R

L

I

K

E

P

P

G

-

A

-

G

-

E

-

L

L

Q

Q

L

L

T

T

D

D

A

A

I

V

Q

A

T

L

L

L

L

I

Q

Q

E

E

E

G

Q

H

-

P

V

V

L

H

A

V

Y

V

R

V

Y

H

R

R

G

G

T

D

R

R

Y

H

D

D

C

L

G

G

S

N

K

P

V

G

G

G

Y

F

L

L

K

R

A

A

T

A

V

V

E

D

Y

F

A

A

L

L

R

Q

H

D

P

P

E

D

V

Y

R

G

E

P

E

E

F

L

T

R

E

A

Y

W

I

L

R

T

-

D

Q

R

I

I

A

A

L

R

P

P

binding uridine-5'-diphosphate-glucose: P8 (= P10), A10 (= A12), G11 (= G13), Q102 (= Q105), A105 (≠ T108), G107 (= G110), A111 (= A114), L130 (= L131), P131 (≠ A132), D133 (= D134), A203 (≠ V204), G205 (= G206)

5z09A St0452(y97n)-utp binding form (see paper)
28% identity, 85% coverage: 6:257/296 of query aligns to 2:210/401 of 5z09A

query
sites
5z09A

K

K

A

A

V

F

F

I

P
x
L

V
x
A

A
|
A

G
|
G

L
x
S

G
|
G

T
x
E

R
|
R

F

L

L

E

P

P

A

I

T

T

K

H

A

T

S

R

P

P

K

K

E

A

M

F

L

V

P

P

V

I

V

L

D

S

K

K

P

P

L

L

I

I

Q

E

Y

Y

A

Q

V

I

E

E

E

Y

A

L

A

R

A

K

A

C

G

G

I

I

T

R

E

D

M

I

-

T

I

V

F

I

V

V

T

S

G

S

R

K

N

N

K

K

R

-

A

-

I

-

E

-

D

E

H

Y

F

F

D

E

K

K

A

-

Y

-

E

-

L

-

E

-

T

-

E

-

L

-

E

-

A

-

N

-

D

-

K

-

Q

-

T

-

L

-

N

-

T

-

V

-

R

-

S

-

I

-

K

K

P

L

P

K

Q

E

M

I

A

S

C

I

V

V

Y

-

V

T

R
x
Q

Q

K

P

D

E

D

T

I

L

K

G
|
G

L

T

G

G

H

A

A

A

V

I

L

L

C

S

A

A

E

K

T

F

L

-

V

-

G

-

D

-

N

N

P

D

F

E

A

A

V

L

I

I

L

I

A

N

D
x
G

D
|
D

L

L

I

F

A

F

G

S

E

N

P

E

P

K

A

E

L

I

S

C

Q

N

M

I

L

I

E

T

A

L

F

K

D

E

H

N

Y

-

H

-

S

-

S

A

V

I

L

I

G

G

V

V

E

K

E

-

I

-

P

-

R

V

E

S

R

N

T

P

R

K

A

D

Y

Y

G

G

V

V

I

L

D

-

G

-

K

-

A

-

W

V

D

L

D

D

S

N

I

Q

I

N

K

N

L

L

S

S

G

K

I

I

V

I

E

E

K

K

P

P

A

-

P

-

D

E

D

I

A

P

P

P

S

S

N

N

L

L

G

I

V

N

V

A

G

G

R

I

Y

Y

V

K

L

L

M

N

P

S

T

D

I

I

F

F

R

T

H

Y

L

L

R

D

R

K

L

I

K

S

P

I

G

S

A

E

G

R

G

G

E

E

L

L

Q

E

L

L

T

T

D

D

A

A

I

I

Q

N

T

L

L

M

L

A

Q

K

E

D

E

H

Q

R

V

V

L

K

A

V

Y

I

R

E

Y

Y

R

E

G

G

T

Y

R

W

Y

M

D

D

C

I

G

G

binding uridine 5'-triphosphate: L6 (≠ P10), A7 (≠ V11), A8 (= A12), G9 (= G13), S10 (≠ L14), G11 (= G15), E12 (≠ T16), R13 (= R17), Q73 (≠ R104), G79 (= G110), G98 (≠ D133), D99 (= D134)

2ggqA Complex of hypothetical glucose-1-phosphate thymidylyltransferase from sulfolobus tokodaii
28% identity, 85% coverage: 6:257/296 of query aligns to 2:210/401 of 2ggqA

query
sites
2ggqA

K

K

A

A

V

F

F

I

P
x
L

V

A

A
|
A

G
|
G

L
x
S

G
|
G

T
x
E

R
|
R

F

L

L

E

P

P

A

I

T

T

K

H

A

T

S

R

P

P

K
|
K

E

A

M

F

L

V

P

P

V

I

V

L

D

S

K

K

P

P

L

L

I

I

Q

E

Y

Y

A

Q

V

I

E

E

E

Y

A

L

A

R

A

K

A

C

G

G

I

I

T

R

E

D

M

I

-

T

I

V

F

I

V

V

T

S

G

S

R

K

N

N

K

K

R

-

A

-

I

-

E

-

D

E

H

Y

F

F

D

E

K

K

A

-

Y

-

E

-

L

-

E

-

T

-

E

-

L

-

E

-

A

-

N

-

D

-

K

-

Q

-

T

-

L

-

N

-

T

-

V

-

R

-

S

-

I

-

K

K

P

L

P

K

Q

E

M

I

A

S

C

I

V

V

Y

-

V

T

R
x
Q

Q

K

P

D

E

D

T

I

L

K

G
|
G

L

T

G

G

H

A

A
|
A

V

I

L

L

C

S

A

A

E

K

T

F

L

-

V

-

G

-

D

-

N

N

P

D

F

E

A

A

V

L

I

I

L

I

A

Y

D

G

D

D

L

L

I

F

A

F

G

S

E

N

P

E

P

K

A

E

L

I

S

C

Q

N

M

I

L

I

E

T

A

L

F

K

D

E

H

N

Y

-

H

-

S

-

S

A

V

I

L

I

G

G

V

V

E

K

E

-

I

-

P

-

R

V

E

S

R

N

T

P

R

K

A

D

Y

Y

G

G

V

V

I

L

D

-

G

-

K

-

A

-

W

V

D

L

D

D

S

N

I

Q

I

N

K

N

L

L

S

S

G

K

I

I

V

I

E

E

K

K

P

P

A

-

P

-

D

E

D

I

A

P

P

P

S

S

N

N

L

L

G

I

V

N

V

A

G

G

R

I

Y

Y

V

K

L

L

M

N

P

S

T

D

I

I

F

F

R

T

H

Y

L

L

R

D

R

K

L

I

K

S

P

I

G

S

A

E

G
x
R

G

G

E
|
E

L

L

Q

E

L

L

T

T

D

D

A

A

I

I

Q

N

T

L

L

M

L

A

Q

K

E

D

E

H

Q

R

V

V

L

K

A

V

Y

I

R

E

Y

Y

R

E

G

G

T

Y

R

W

Y

M

D

D

C

I

G

G

active site: R13 (= R17)
binding thymidine-5'-triphosphate: L6 (≠ P10), A8 (= A12), G9 (= G13), S10 (≠ L14), G11 (= G15), E12 (≠ T16), R13 (= R17), K23 (= K27), Q73 (≠ R104), G79 (= G110), A83 (= A114), R179 (≠ G226), E181 (= E228)

Sites not aligning to the query:

binding thymidine-5'-triphosphate: 344

Q975F9 Bifunctional sugar-1-phosphate nucleotidylyltransferase/acetyltransferase; EC 2.7.7.24; EC 2.7.7.9; EC 2.7.7.83; EC 2.7.7.23; EC 2.3.1.276; EC 2.3.1.157 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 3 papers)
28% identity, 85% coverage: 6:257/296 of query aligns to 2:210/401 of Q975F9

query
sites
Q975F9

K

K

A

A

V

F

F

I

P

L

V

A

A
|
A

G
|
G

L
x
S

G
|
G

T
x
E

R
|
R

F

L

L

E

P

P

A

I

T

T

K

H

A

T

S

R

P

P

K

K

E

A

M

F

L

V

P

P

V

I

V

L

D

S

K

K

P

P

L

L

I

I

Q

E

Y

Y

A

Q

V

I

E

E

E

Y

A

L

A

R

A

K

A

C

G

G

I

I

T

R

E

D

M

I

-

T

I

V

F

I

V

V

T

S

G

S

R

K

N

N

K

K

R

-

A

-

I

-

E

-

D

E

H

Y

F

F

D

E

K

K

A

-

Y

-

E

-

L

-

E

-

T

-

E

-

L

-

E

-

A

-

N

-

D

-

K

-

Q

-

T

-

L

-

N

-

T

-

V

-

R

-

S

-

I

-

K

K

P

L

P

K

Q

E

M

I

A

S

C

I

V

V

Y

-

V

T

R
x
Q

Q

K

P

D

E

D

T

I

L

K

G
|
G

L
x
T

G

G

H

A

A

A

V

I

L

L

C

S

A

A

E

K

T

F

L

-

V

-

G

-

D

-

N

N

P

D

F

E

A

A

V

L

I

I

L

I

A
x
Y

D

G

D

D

L

L

I

F

A

F

G

S

E

N

P

E

P

K

A

E

L

I

S

C

Q

N

M

I

L

I

E

T

A

L

F

K

D

E

H

N

Y

-

H

-

S

-

S

A

V

I

L

I

G

G

V

V

E

K

E

-

I

-

P

-

R

V

E

S

R

N

T

P

R

K

A

D

Y

Y

G

G

V

V

I

L

D

-

G

-

K

-

A

-

W

V

D

L

D

D

S

N

I

Q

I

N

K

N

L

L

S

S

G

K

I

I

V

I

E
|
E

K

K

P

P

A

-

P

-

D

E

D

I

A

P

P

P

S

S

N

N

L

L

G

I

V

N

V

A

G

G

R

I

Y

Y

V

K

L

L

M

N

P

S

T

D

I

I

F

F

R

T

H

Y

L

L

R

D

R

K

L

I

K

S

P

I

G

S

A

E

G

R

G

G

E

E

L

L

Q

E

L

L

T

T

D

D

A

A

I

I

Q

N

T

L

L

M

L

A

Q

K

E

D

E

H

Q

R

V

V

L

K

A

V

Y

I

R

E

Y

Y

R

E

G

G

T

Y

R

W

Y

M

D

D

C

I

G

G

AGSGER 8:13 (≠ AGLGTR 12:17) binding a ribonucleoside 5'-triphosphate
Q73 (≠ R104) binding a ribonucleoside 5'-triphosphate
G79 (= G110) binding a ribonucleoside 5'-triphosphate
T80 (≠ L111) mutation T->A,G,Q: Increases both GlcNAc-1-P UTase and Glc-1-P UTase activities.;