SitesBLAST
Comparing WP_047213848.1 NCBI__GCF_001931675.1:WP_047213848.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
5ylnA Zinc dependent alcohol dehydrogenase 2 from streptococcus pneumonia - apo form
57% identity, 99% coverage: 1:343/345 of query aligns to 5:341/348 of 5ylnA
4cpdA Alcohol dehydrogenase tadh from thermus sp. Atn1
35% identity, 99% coverage: 1:342/345 of query aligns to 1:345/346 of 4cpdA
- active site: C38 (= C38), G39 (= G39), S40 (≠ T40), H43 (= H43), H59 (= H60), E60 (= E61), C89 (= C90), C92 (= C93), C95 (= C96), C103 (= C104), G107 (= G108), D152 (= D149), T156 (= T153), K340 (≠ Q337)
- binding nicotinamide-adenine-dinucleotide: G39 (= G39), S40 (≠ T40), T156 (= T153), G178 (= G176), P179 (= P177), V180 (≠ I178), D200 (= D198), R201 (= R199), R205 (= R203), A243 (= A242), V244 (= V243), V266 (= V265), V268 (= V267), L292 (= L290), A293 (≠ V291), F333 (= F330)
- binding zinc ion: C38 (= C38), H59 (= H60), C89 (= C90), C92 (= C93), C95 (= C96), C103 (= C104), D152 (= D149)
6schC Nadh-dependent variant of cbadh (see paper)
29% identity, 94% coverage: 1:324/345 of query aligns to 1:334/355 of 6schC
- active site: C37 (= C38), S39 (≠ T40), H42 (= H43), H59 (= H60), D150 (= D149)
- binding nicotinamide-adenine-dinucleotide: T38 (≠ G39), W110 (= W109), D150 (= D149), T154 (= T153), G174 (= G174), V178 (≠ I178), D198 (= D198), Y199 (≠ R199), R200 (≠ D200), A242 (= A242), G243 (≠ V243), G244 (= G244), N266 (≠ G266), Y267 (≠ V267)
- binding zinc ion: C37 (= C38), H59 (= H60), D150 (= D149)
Sites not aligning to the query:
O58389 L-threonine 3-dehydrogenase; L-ThrDH; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 2 papers)
32% identity, 95% coverage: 1:328/345 of query aligns to 5:336/348 of O58389
- C42 (= C38) binding Zn(2+)
- T44 (= T40) mutation to A: Total loss of enzymatic activity.
- H67 (= H60) binding Zn(2+)
- E68 (= E61) binding Zn(2+)
- C97 (= C90) binding Zn(2+)
- C100 (= C93) binding Zn(2+)
- C103 (= C96) binding Zn(2+)
- C111 (= C104) binding Zn(2+)
- E152 (≠ D149) mutation E->A,Q: Almost complete loss of enzymatic activity.; mutation to C: 120-fold decrease in catalytic efficiency.; mutation to D: Shows 3-fold higher turnover rate and reduced affinities toward L-threonine and NAD(+), compared to wild-type.; mutation to K: Total loss of enzymatic activity.
- L179 (≠ I178) binding NAD(+)
- E199 (≠ D198) binding NAD(+); mutation to A: Large decrease in affinity for NAD(+).
- R204 (= R203) binding NAD(+); mutation to A: Large decrease in affinity for NAD(+).
- LGL 266:268 (≠ VGV 265:267) binding NAD(+)
- IT 291:292 (= IT 286:287) binding NAD(+)
- R294 (= R289) mutation to A: 4000-fold decrease in catalytic efficiency.
2dfvA Hyperthermophilic threonine dehydrogenase from pyrococcus horikoshii (see paper)
32% identity, 95% coverage: 1:328/345 of query aligns to 3:334/346 of 2dfvA
- active site: C40 (= C38), G41 (= G39), T42 (= T40), H45 (= H43), H65 (= H60), E66 (= E61), C95 (= C90), C98 (= C93), C101 (= C96), C109 (= C104), K113 (≠ G108), P151 (≠ I150), A155 (≠ G154)
- binding nicotinamide-adenine-dinucleotide: G175 (= G176), P176 (= P177), L177 (≠ I178), E197 (≠ D198), P198 (≠ R199), R202 (= R203), F241 (≠ A242), S242 (≠ V243), A244 (≠ V245), L264 (≠ V265), G265 (= G266), L266 (≠ V267), I289 (= I286), T290 (= T287)
- binding zinc ion: C95 (= C90), C101 (= C96), C109 (= C104)
Sites not aligning to the query:
1h2bB Crystal structure of the alcohol dehydrogenase from the hyperthermophilic archaeon aeropyrum pernix at 1.65a resolution (see paper)
30% identity, 92% coverage: 6:324/345 of query aligns to 9:337/344 of 1h2bB
- active site: C39 (= C38), H40 (≠ G39), T41 (= T40), H44 (= H43), H64 (= H60), E65 (= E61), D94 (≠ C90), C97 (= C93), C100 (= C96), C108 (= C104), E112 (≠ G108), D153 (≠ P152), T157 (≠ E156)
- binding nicotinamide-adenine-dinucleotide (acidic form): C39 (= C38), H40 (≠ G39), T41 (= T40), H44 (= H43), T157 (≠ E156), V180 (≠ A175), G181 (= G176), G182 (≠ P177), L183 (≠ I178), D203 (= D198), V204 (≠ R199), K208 (≠ R203), F246 (≠ A242), V247 (= V243), T252 (= T248), Y255 (≠ L251), V269 (= V265), G270 (= G266), Y271 (≠ V267), L293 (≠ W280), V294 (≠ D281)
- binding octanoic acid (caprylic acid): T41 (= T40), W50 (≠ V49), H64 (= H60), D153 (≠ P152), V294 (≠ D281)
- binding zinc ion: C39 (= C38), H64 (= H60), D94 (≠ C90), C97 (= C93), C100 (= C96), C108 (= C104), D153 (≠ P152)
Sites not aligning to the query:
7f3pD Crystal structure of a NADP-dependent alcohol dehydrogenase mutant in apo form (see paper)
31% identity, 90% coverage: 15:325/345 of query aligns to 20:334/355 of 7f3pD
3fsrA Chimera of alcohol dehydrogenase by exchange of the cofactor binding domain res 153-295 of t. Brockii adh by c. Beijerinckii adh (see paper)
29% identity, 90% coverage: 15:325/345 of query aligns to 17:331/352 of 3fsrA
- active site: C37 (= C38), T38 (≠ G39), S39 (≠ T40), H42 (= H43), H59 (= H60), E60 (= E61), D89 (≠ C90), T92 (≠ C93), V95 (≠ C96), S103 (≠ C104), D150 (= D149), T154 (= T153)
- binding zinc ion: C37 (= C38), H59 (= H60), D150 (= D149)
Sites not aligning to the query:
1ykfA NADP-dependent alcohol dehydrogenase from thermoanaerobium brockii (see paper)
31% identity, 90% coverage: 15:325/345 of query aligns to 17:331/352 of 1ykfA
- active site: C37 (= C38), T38 (≠ G39), S39 (≠ T40), H42 (= H43), H59 (= H60), E60 (= E61), D89 (≠ C90), T92 (≠ C93), V95 (≠ C96), S103 (≠ C104), D150 (= D149), T154 (= T153)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: S39 (≠ T40), D150 (= D149), T154 (= T153), G174 (= G174), I175 (≠ A175), G176 (= G176), P177 (= P177), V178 (≠ I178), S199 (≠ R199), R200 (≠ D200), Y218 (≠ P218), I223 (≠ P223), N266 (≠ G266), Y267 (≠ V267)
- binding zinc ion: C37 (= C38), H59 (= H60), D150 (= D149)
Sites not aligning to the query:
1bxzB Crystal structure of a thermophilic alcohol dehydrogenase substrate complex from thermoanaerobacter brockii (see paper)
31% identity, 90% coverage: 15:325/345 of query aligns to 17:331/352 of 1bxzB
- active site: C37 (= C38), T38 (≠ G39), S39 (≠ T40), H42 (= H43), H59 (= H60), E60 (= E61), D89 (≠ C90), T92 (≠ C93), V95 (≠ C96), S103 (≠ C104), D150 (= D149), T154 (= T153)
- binding 2-butanol: H59 (= H60), D150 (= D149)
Sites not aligning to the query:
P14941 NADP-dependent isopropanol dehydrogenase; EC 1.1.1.80 from Thermoanaerobacter brockii (Thermoanaerobium brockii) (see 2 papers)
31% identity, 90% coverage: 15:325/345 of query aligns to 17:331/352 of P14941
- C37 (= C38) binding Zn(2+)
- H59 (= H60) binding Zn(2+)
- D150 (= D149) binding Zn(2+)
- IGPV 175:178 (≠ AGPI 175:178) binding NADP(+)
- GSR 198:200 (≠ DRD 198:200) binding NADP(+)
- Y218 (≠ P218) binding NADP(+)
- VNY 265:267 (≠ VGV 265:267) binding NADP(+)
Sites not aligning to the query:
1kevA Structure of NADP-dependent alcohol dehydrogenase (see paper)
29% identity, 94% coverage: 1:324/345 of query aligns to 1:334/351 of 1kevA
- active site: C37 (= C38), T38 (≠ G39), S39 (≠ T40), H42 (= H43), H59 (= H60), E60 (= E61), D89 (≠ C90), S92 (≠ C93), V95 (≠ C96), S103 (≠ C104), D150 (= D149), T154 (= T153)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: T38 (≠ G39), D150 (= D149), I175 (≠ A175), G176 (= G176), V178 (≠ I178), S199 (≠ R199), R200 (≠ D200), Y218 (≠ P218), A242 (= A242), G244 (= G244), N266 (≠ G266), Y267 (≠ V267)
- binding zinc ion: C37 (= C38), H59 (= H60), D150 (= D149)
Sites not aligning to the query:
P25984 NADP-dependent isopropanol dehydrogenase; CbADH; EC 1.1.1.80 from Clostridium beijerinckii (Clostridium MP) (see 3 papers)
29% identity, 94% coverage: 1:324/345 of query aligns to 1:334/351 of P25984