SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_047214274.1 NCBI__GCF_001931675.1:WP_047214274.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P9WID3 ATP-dependent 6-phosphofructokinase isozyme 2; ATP-PFK 2; Phosphofructokinase 2; Phosphofructokinase B; Phosphohexokinase 2; Tagatose-6-phosphate kinase; EC 2.7.1.11; EC 2.7.1.144 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
45% identity, 97% coverage: 2:305/315 of query aligns to 12:314/339 of P9WID3

query
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P9WID3

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K283 (≠ E274) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

3uqdB Crystal structure of the phosphofructokinase-2 from escherichia coli in complex with substrates and products (see paper)
39% identity, 97% coverage: 1:304/315 of query aligns to 1:304/309 of 3uqdB

query
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3uqdB

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active site: G253 (= G253), A254 (= A254), G255 (= G255), D256 (= D256)
binding adenosine-5'-diphosphate: K185 (= K185), N187 (≠ S187), S224 (≠ T224), G226 (= G226), P227 (≠ G227), G229 (= G229), S248 (≠ M248), M258 (≠ F258), V280 (≠ M280), G283 (≠ A283), S284 (≠ A284)
binding adenosine-5'-triphosphate: Y23 (≠ V23), K27 (= K27)
binding 6-O-phosphono-beta-D-fructofuranose: K27 (= K27), R29 (= R29)
binding 1,6-di-O-phosphono-beta-D-fructofuranose: D14 (= D14), G39 (= G39), N43 (= N43), R90 (= R90), R105 (= R105), S139 (≠ R140), G253 (= G253)

3uqdA Crystal structure of the phosphofructokinase-2 from escherichia coli in complex with substrates and products (see paper)
39% identity, 97% coverage: 1:304/315 of query aligns to 1:304/309 of 3uqdA

query
sites
3uqdA

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active site: G253 (= G253), A254 (= A254), G255 (= G255), D256 (= D256)
binding adenosine-5'-triphosphate: K185 (= K185), S224 (≠ T224), G226 (= G226), P227 (≠ G227), G229 (= G229), T251 (≠ A251), G255 (= G255), M258 (≠ F258), V280 (≠ M280), G283 (≠ A283), S284 (≠ A284), T287 (≠ L287)
binding 6-O-phosphono-beta-D-fructofuranose: D14 (= D14), G38 (= G38), G39 (= G39), N43 (= N43), R90 (= R90), S139 (≠ R140), D256 (= D256)
binding 1,6-di-O-phosphono-beta-D-fructofuranose: K27 (= K27), R29 (= R29)

3n1cA Crystal structure of the phosphofructokinase-2 from escherichia coli in complex with fructose-6-phosphate (see paper)
39% identity, 97% coverage: 1:304/315 of query aligns to 1:304/309 of 3n1cA

query
sites
3n1cA

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E

Q

R

K

F

I

Y

Y

active site: G253 (= G253), A254 (= A254), G255 (= G255), D256 (= D256)
binding 6-O-phosphono-beta-D-fructofuranose: D14 (= D14), K27 (= K27), R29 (= R29), G39 (= G39), N43 (= N43), R90 (= R90), G138 (= G139), S139 (≠ R140), D256 (= D256)

P06999 ATP-dependent 6-phosphofructokinase isozyme 2; ATP-PFK 2; Phosphofructokinase 2; 6-phosphofructokinase isozyme II; Phosphohexokinase 2; EC 2.7.1.11 from Escherichia coli (strain K12) (see 3 papers)
39% identity, 97% coverage: 1:304/315 of query aligns to 1:304/309 of P06999

query
sites
P06999

M

M

P

V

E

R

I

I

L

Y

T

T

F

L

T

T

P

L

N

A

P

P

S

S

I

L

D

D

V

S

F

A

T

T

T

I

V

T

E

P

R

Q

V

I

V

Y

D

P

T

E

R

G

K
|
K

M

L

R

R

C

C

G

T

A

A

E

P

R

V

R

F

D

E

P

P

G

G

V

I

N

N

V

V

A

A

R

R

V

A

I

I

Q

A

R

H

L

L

G

G

D

S

C

A

T

T

A

A

V

I

V

F

M

P

A

A

G

G

Q

A

T

T

G

G

E

E

M

H

L

L

R

V

Q

S

L

L

A

A

A

D

E

E

K

N

V

V

P

P

T

V

A

A

C

T

V

V

G

E

I

A

A

K

G

D

H

W

T

T

R

R

E

Q

S

N

F

L

S

H

V

V

L

H

E

V

T

E

G

A

T

S

G

G

R

E

E

Q

F

Y

R

R

F

F

V

V

L

M

P

P

G

G

P

A

V

A

I

L

T

N

G

E

N

D

D

E

W

F

Q

R

R

Q

C

L

H

E

D

E

A

Q

L

V

A

L

A

E

F

I

D

E

P

S

A

G

P

A

R

-

F

I

L

L

V

V

L

I

S

S

G

G

R

S

L

L

P

P

G

G

A

V

P

K

V

L

D

E

G

K

Y

L

A

T

Q

Q

L

L

A

I

R

S

A

A

A

Q

A

K

R

Q

G

G

T

I

Q

R

V

C

A

I

I

V

D

D

T

S

S

S

G

G

P

E

A

A

L

L

A

S

A

A

L

L

E

A

A

I

G

G

-

N

V

I

A

E

I

L

V

V

K
|
K

P
|
P

S
x
N

V
x
Q

D
x
K

E
|
E

L

L

R

S

E

A

L

L

T

V

G

N

Q

R

P

E

L

L

T

T

D

Q

E

P

T

D

Q

D

W

V

C

R

A

K

A

A

R

Q

Q

E

I

I

V

V

R

N

D

S

G

G

R

K

A

A

R

K

M

R

V

V

A

V

L

V

T
x
S

L
|
L

G
|
G

G
x
P

R
x
Q

G
|
G

A

A

L

L

L

G

V

V

E

D

R

S

H

E

R

N

V

C

L

I

R

Q

A

V

P

V

A

P

L

P

P

P

V

V

K

K

M
x
S

V

Q

S
|
S

A

T

I
x
V

G

G

A

A

G

G

D

D

S

S

F

M

L

V

A

G

A

A

L

M

V

T

W

L

A

K

L

L

N

A

R

E

G

N

A

A

G

S

T

L

G

E

E

E

A

M

F

V

R

R

Y

F

G

G

M
x
V

A

A

A

G

A
x
S

A

A

A
|
A

L

T

L

L

S
x
N

A

Q

G
|
G

T

T

G
x
R

L

L

C

C

H

S

K

H

E

D

D

D

V

T

E

Q

R

K

F

I

Y

Y

K27 (= K27) binding ATP; binding ATP
KPN 185:187 (≠ KPS 185:187) binding in other chain
NQK 187:189 (≠ SVD 187:189) binding in other chain
E190 (= E190) mutation to Q: Causes a 50-fold decrease in the kcat value and a 15-fold increment in the apparent KM for ATP.
SLGPQG 224:229 (≠ TLGGRG 224:229) binding in other chain
S248 (≠ M248) binding in other chain
S250 (= S250) binding K(+)
V252 (≠ I252) binding K(+)
V280 (≠ M280) binding in other chain
S284 (≠ A284) binding in other chain
A286 (= A286) binding K(+)
N289 (≠ S289) binding K(+)
G291 (= G291) binding K(+)
R293 (≠ G293) binding K(+)

3cqdA Structure of the tetrameric inhibited form of phosphofructokinase-2 from escherichia coli (see paper)
39% identity, 97% coverage: 1:304/315 of query aligns to 1:299/304 of 3cqdA

query
sites
3cqdA

M

M

P

V

E

R

I

I

L

Y

T

T

F

L

T

T

P

L

N

A

P

P

S

S

I

L

D

D

V

S

F

A

T

T

T

I

V

T

E

P

R

Q

V

I

V
x
Y

D

P

T

E

R
x
G

K
|
K

M

L

R

R

C

C

G

T

A

A

E

P

R

V

R

F

D

E

P

P

G

G

V

I

N

N

V

V

A

A

R

R

V

A

I

I

Q

A

R

H

L

L

G

G

D

S

C

A

T

T

A

A

V

I

V

F

M

P

A

A

G

G

Q

A

T

T

G

G

E

E

M

H

L

L

R

V

Q

S

L

L

A

A

A

D

E

E

K

N

V

V

P

P

T

V

A

A

C

T

V

V

G

E

I

A

A

K

G

D

H

W

T

T

R

R

E

Q

S

N

F

L

S

H

V

V

L

H

E

V

T

E

G

A

T

S

G

G

R

E

E

Q

F

Y

R

R

F

F

V

V

L

M

P

P

G

G

P

A

V

A

I

L

T

N

G

E

N

D

D

E

W

F

Q

R

R

Q

C

L

H

E

D

E

A

Q

L

V

A

L

A

E

F

I

D

E

P

S

A

G

P

A

R

-

F

I

L

L

V

V

L

I

S

S

G

G

R

S

L

L

P

P

G

G

A

V

P

K

V

L

D

E

G

K

Y

L

A

T

Q

Q

L

L

A

I

R

S

A

A

A

Q

A

K

R

Q

G

G

T

I

Q

R

V

C

A

I

I

V

D

D

T

S

S

S

G

G

P

E

A

A

L

L

A

S

A

A

L

L

E

A

A

I

G

G

-

N

V

I

A

E

I

L

V

V

K
|
K

P

P

S
x
N

V

Q

D
x
K

E

E

L

L

R

S

E

A

L

L

T

V

G

N

Q

R

P

E

L

L

T

T

D

Q

E

P

T

D

Q

D

W

V

C

R

A

K

A

A

R

Q

Q

E

I

I

V

V

R

N

D

S

G

G

R

K

A

A

R

K

M

R

V

V

A

V

L

V

T
x
S

L

L

G
|
G

G
x
P

R

Q

G
|
G

A

A

L

L

L

G

V

V

E

D

R

S

H

E

R

N

V

C

L

I

R

Q

A

V

P

V

A

P

L

P

P

P

V

V

K

K

M
x
S

V

Q

S

S

A
x
T

I

V

G
|
G

A
|
A

G
|
G

D
|
D

S

S

F
x
M

L

V

A

G

A

A

L

M

V

T

W

L

A

K

L

L

N

A

R

E

G

N

A

A

G

S

T

L

G

E

E

E

A

M

F

V

R

R

Y

F

G

G

M
x
V

A

A

A
x
G

A
x
S

A

A

L
x
T

L

L

S

-

A

-

G

-

T

-

G

-

L

L

C

C

H

S

K

H

E

D

D

D

V

T

E

Q

R

K

F

I

Y

Y

active site: G253 (= G253), A254 (= A254), G255 (= G255), D256 (= D256)
binding adenosine-5'-triphosphate: Y23 (≠ V23), G26 (≠ R26), K27 (= K27), K185 (= K185), N187 (≠ S187), N187 (≠ S187), K189 (≠ D189), S224 (≠ T224), G226 (= G226), P227 (≠ G227), G229 (= G229), S248 (≠ M248), T251 (≠ A251), A254 (= A254), G255 (= G255), M258 (≠ F258), V280 (≠ M280), G283 (≠ A283), S284 (≠ A284), T287 (≠ L287)

3uqeA Crystal structure of the phosphofructokinase-2 mutant y23d from escherichia coli
39% identity, 97% coverage: 1:304/315 of query aligns to 1:302/307 of 3uqeA

query
sites
3uqeA

M

M

P

V

E

R

I

I

L

Y

T

T

F

L

T

T

P

L

N

A

P

P

S

S

I

L

D

D

V

S

F

A

T

T

T

I

V

T

E

P

R

Q

V

I

V

D

D

P

T

E

R

G

K

K

M

L

R

R

C

C

G

T

A

A

E

P

R

V

R

F

D

E

P

P

G

G

V

I

N

N

V

V

A

A

R

R

V

A

I

I

Q

A

R

H

L

L

G

G

D

S

C

A

T

T

A

A

V

I

V

F

M

P

A

A

G

G

Q

A

T

T

G

G

E

E

M

H

L

L

R

V

Q

S

L

L

A

A

A

D

E

E

K

N

V

V

P

P

T

V

A

A

C

T

V

V

G

E

I

A

A

K

G

D

H

W

T

T

R

R

E

Q

S

N

F

L

S

H

V

V

L

H

E

V

T

E

G

A

T

S

G

G

R

E

E

Q

F

Y

R

R

F

F

V

V

L

M

P

P

G

G

P

A

V

A

I

L

T

N

G

E

N

D

D

E

W

F

Q

R

R

Q

C

L

H

E

D

E

A

Q

L

V

A

L

A

E

F

I

D

E

P

S

A

G

P

A

R

-

F

I

L

L

V

V

L

I

S

S

G

G

R

S

L

L

P

P

G

G

A

V

P

K

V

L

D

E

G

K

Y

L

A

T

Q

Q

L

L

A

I

R

S

A

A

A

Q

A

K

R

Q

G

G

T

I

Q

R

V

C

A

I

I

V

D

D

T

S

S

S

G

G

P

E

A

A

L

L

A

S

A

A

L

L

E

A

A

I

G

G

-

N

V

I

A

E

I

L

V

V

K
|
K

P

P

S
x
N

V

Q

D
x
K

E

E

L

L

R

S

E

A

L

L

T

V

G

N

Q

R

P

E

L

L

T

T

D

Q

E

P

T

D

Q

D

W

V

C

R

A

K

A

A

R

Q

Q

E

I

I

V

V

R

N

D

S

G

G

R

K

A

A

R

K

M

R

V

V

A

V

L

V

T
x
S

L

L

G
|
G

G
x
P

R

Q

G
|
G

A

A

L

L

L

G

V

V

E

D

R

S

H

E

R

N

V

C

L

I

R

Q

A

V

P

V

A

P

L

P

P

P

V

V

K

K

M
x
S

V

Q

S

S

A

T

I

V

G
|
G

A
|
A

G
|
G

D
|
D

S

S

F
x
M

L

V

A

G

A

A

L

M

V

T

W

L

A

K

L

L

N

A

R

E

G

N

A

A

G

S

T

L

G

E

E

E

A

M

F

V

R

R

Y

F

G

G

M
x
V

A

A

A
x
G

A
x
S

A

A

L
x
T

L

L

S

N

A

-

G

-

T

Q

G

R

L

L

C

C

H

S

K

H

E

D

D

D

V

T

E

Q

R

K

F

I

Y

Y

active site: G253 (= G253), A254 (= A254), G255 (= G255), D256 (= D256)
binding adenosine-5'-triphosphate: K185 (= K185), N187 (≠ S187), S224 (≠ T224), G226 (= G226), P227 (≠ G227), G229 (= G229), S248 (≠ M248), A254 (= A254), G255 (= G255), M258 (≠ F258), V280 (≠ M280), G283 (≠ A283), S284 (≠ A284), T287 (≠ L287)
binding pyrophosphate 2-: N187 (≠ S187), K189 (≠ D189)

2f02A Crystal structure of lacc from enterococcus faecalis in complex with atp
28% identity, 97% coverage: 4:307/315 of query aligns to 3:307/319 of 2f02A

query
sites
2f02A

I

I

L

V

T

T

F

V

T

T

P

M

N

N

P

P

S

S

I

I

D

D

V

I

F

S

T

Y

T

L

V

L

E

D

R

H

V

L

V

K

D

L

T

D

R

T

K

V

M

N

R

R

C

T

G

S

A

Q

E

V

R

T

R

K

D

T

P

P

G

G

G

K

G

G

V

L

N

N

V

V

A

T

R

R

V

V

I

I

Q

H

R

D

L

L

G

G

D

D

C

V

T

I

A

A

V

T

V

G

M

V

A

L

G

G

Q

F

T

H

G

G

E

A

M

F

L

I

R

A

Q

N

L

E

L

L

A

K

E

A

K

N

V

I

P

P

T

Q

A

A

C

F

V

T

G

S

I

I

A

K

G

E

H

E

T

T

R

R

E

D

S

S

F

I

S

A

V

I

L

L

E

H

T

E

G

G

T

N

G

Q

R

T

E

E

F

-

R

-

F

I

V

L

L

E

P

A

G

G

P

P

V

T

I

V

T

S

G

P

N

E

D

E

W

I

Q

S

R

N

C

F

H

L

D

E

A

N

L

F

A

D

A

Q

F

L

D

I

P

K

A

Q

P

A

R

E

F

I

L

V

V

T

L

I

S

S

G

G

R

S

L

L

P

A

P

K

G

G

A

L

P

P

V

S

D

D

G

F

Y

Y

A

Q

Q

E

L

L

A

V

R

Q

A

K

A

A

A

H

A

A

R

Q

G

E

T

V

Q

K

V

V

A

L

I

L

D

D

T

T

S

S

G

G

P

D

A

S

L

L

A

R

A

Q

A

V

L

L

E

Q

A

G

-

P

-

W

G

K

V

P

A

Y

I

L

V

I

K

K

P

P

S
x
N

V

L

D

E

E

E

L

L

R

E

E

G

L

L

T

L

G

G

Q

Q

P

D

L

F

T

S

D

-

E

E

T

N

Q

P

W

L

C

A

A

A

A

V

A

Q

R

T

Q

A

I

L

V

T

R

K

D

P

G

M

R

F

A

A

-

G

-

I

R

E

M

W

V

I

A

V

L

I

T
x
S

L

L

G
|
G

G

K

R

D

G

G

A

A

L

I

L

A

V

K

E

H

R

H

H

D

R

Q

V

F

L

Y

R

R

A

V

P

K

A
x
I

L

P

P

T

V
x
I

K

Q

M

A

V

K

S

N

A

P

I

V

G
|
G

A
x
S

G
|
G

D

D

S

A

F
x
T

L

I

A

A

A

G

L

L

V

A

W

Y

A

G

L

L

N

A

R

K

G

D

A

A

G

P

T

A

G

A

E

E

A

L

F

L

R

K

Y

W

G

G

M
|
M

A

A

A
x
G

A
x
M

A

A

A

N

L

A

L

Q

S

E

A

R

G

M

T

T

G

G

L

H

C

V

H

D

K

V

E

E

D

N

V

V

E

K

R

K

F

H

Y

L

R

M

D

N

M

I

binding adenosine-5'-triphosphate: N186 (≠ S187), S224 (≠ T224), G226 (= G226), I243 (≠ A243), I246 (≠ V246), G253 (= G253), S254 (≠ A254), G255 (= G255), T258 (≠ F258), M280 (= M280), G283 (≠ A283), M284 (≠ A284)

2jg1C Structure of staphylococcus aureus d-tagatose-6-phosphate kinase with cofactor and substrate (see paper)
26% identity, 99% coverage: 4:315/315 of query aligns to 7:314/315 of 2jg1C

query
sites
2jg1C

I

I

L

L

T

T

F

L

T

T

P

L

N

N

P

P

S

S

I

V

D
|
D

V

I

F

S

T

Y

T

P

V

L

E

T

R

A

V

L

V

K

D

L

T

D

R

D

K

V

M

N

R

R

C

V

G

Q

A

E

E

V

R

S

R

K

D

T

P

A

G

G

G
|
G

G
x
K

G

G

V

L

N

N

V

V

A

T

R

R

V

V

I

L

Q

A

R

Q

L

V

G

G

G

E

D

P

C

V

T

L

A

A

V

S

V

G

M

F

A

I

G

G

Q

E

T

L

G

G

E

Q

M

F

L

I

R

A

Q

K

L

K

L

L

A

D

A

H

E

A

K

D

V

I

P

K

T

H

A

A

C

F

V

Y

G

N

I

I

A

K

G

G

H

E

T

T

R
|
R

E

N

S
x
C

F

I

S

A

V

I

L

L

E

H

T

E

G

G

T

Q

G

Q

R

T

E

E

F

-

R

-

F

I

V
x
L

L

E

P

Q

G

G

P

P

V

E

I

I

T

-

G

-

N

-

D

D

W

N

Q

Q

R

E

C

A

H

A

D

G

A

F

L

I

A

K

A

H

F

F

D

E

-

Q

-

M

P

E

A

K

P

V

R

E

F

A

L

V

V

A

L

I

S

S

G
|
G

R
x
S

L

L

P

P

P

K

G

G

A

L

P

N

V

Q

D

D

G

Y

Y

Y

A

A

Q

Q

L

I

A

I

R

E

A

R

A

C

A

Q

A

N

R

K

G

G

T

V

Q

P

V

V

A

I

I

L

D

D

T

C

S

S

G

G

P

A

A

T

L

L

A

Q

A

T

A

V

L

L

E

E

A

N

G

P

V

Y

-

K

-

P

A

T

I

V

V

I

K

K

P

P

S

N

V

I

D

S

E

E

L

L

R

Y

E

Q

L

L

T

L

G

N

Q

Q

P

P

L

L

T

D

D

E

E

S

T

L

Q

E

W

S

C

L

A

K

-

Q

A

A

A

V

R

S

Q

Q

I

P

V

L

R

F

D

E

G

G

R

-

A

I

R

E

M

W

V

I

A

I

L

V

T
x
S

L

L

G
|
G

G
x
A

R

Q

G
|
G

A

A

L

F

L

A

V

K

E

H

R

N

H

H

R

T

V

F

L

Y

R

R

A

V

P

N

A
x
I

L

P

P

T

V
x
I

K

S

M
x
V

V

L

S

N

A

P

I
x
V

G
|
G

A
x
S

G
|
G

D
|
D

S

S

F
x
T

L

V

A

A

A

G

L

I

V

T

W

S

A

A

L

I

N

L

R

N

G

H

A

E

G

N

T

D

G

H

E

D

A

L

F

L

R

K

Y

K

G

A

M
x
N

A

T

A

L

A
x
G

A
x
M

A

L

A

N

L

A

L

Q

S

E

A

A

G

Q

T

T

G

G

L

Y

C

V

H

N

K

L

E

N

D

N

V

-

E

-

R

-

F

-

Y

Y

R

D

D

D

M

L

P

F

S

N

H

Q

A

I

A

E

T

V

L

L

E

E

active site: G256 (= G253), S257 (≠ A254), G258 (= G255), D259 (= D256)
binding phosphoaminophosphonic acid-adenylate ester: S227 (≠ T224), G229 (= G226), A230 (≠ G227), G232 (= G229), I246 (≠ A243), I249 (≠ V246), V251 (≠ M248), V255 (≠ I252), G256 (= G253), S257 (≠ A254), G258 (= G255), D259 (= D256), T261 (≠ F258), N283 (≠ M280), G286 (≠ A283), M287 (≠ A284)
binding 6-O-phosphono-beta-D-tagatofuranose: D17 (= D14), G42 (= G39), K43 (≠ G40), R93 (= R90), C95 (≠ S92), L108 (≠ V107), G140 (= G139), S141 (≠ R140), D259 (= D256)

2jgvB Structure of staphylococcus aureus d-tagatose-6-phosphate kinase in complex with adp (see paper)
26% identity, 99% coverage: 4:315/315 of query aligns to 6:313/314 of 2jgvB

query
sites
2jgvB

I

I

L

L

T

T

F

L

T

T

P

L

N

N

P

P

S

S

I

V

D

D

V

I

F

S

T

Y

T

P

V

L

E

T

R

A

V

L

V

K

D

L

T

D

R

D

K

V

M

N

R

R

C

V

G

Q

A

E

E

V

R

S

R

K

D

T

P

A

G

G

G

K

G

G

V

L

N

N

V

V

A

T

R

R

V

V

I

L

Q

A

R

Q

L

V

G

G

G

E

D

P

C

V

T

L

A

A

V

S

V

G

M

F

A

I

G

G

Q

E

T

L

G

G

E

Q

M

F

L

I

R

A

Q

K

L

K

L

L

A

D

A

H

E

A

K

D

V

I

P

K

T

H

A

A

C

F

V

Y

G

N

I

I

A

K

G

G

H

E

T

T

R

R

E

N

S

C

F

I

S

A

V

I

L

L

E

H

T

E

G

G

T

Q

G

Q

R

T

E

E

F

-

R

-

F

I

V

L

L

E

P

Q

G

G

P

P

V

E

I

I

T

-

G

-

N

-

D

D

W

N

Q

Q

R

E

C

A

H

A

D

G

A

F

L

I

A

K

A

H

F

F

D

E

-

Q

-

L

P

E

A

K

P

V

R

E

F

A

L

V

V

A

L

I

S

S

G

G

R

S

L

L

P

P

P

K

G

G

A

L

P

N

V

Q

D

D

G

Y

Y

Y

A

A

Q

Q

L

I

A

I

R

E

A

R

A

C

A

Q

A

N

R

K

G

G

T

V

Q

P

V

V

A

I

I

L

D

D

T

C

S

S

G

G

P

A

A

T

L

L

A

Q

A

T

A

V

L

L

E

E

A

N

G

P

V

Y

-

K

-

P

A

T

I

V

V

I

K

K

P

P

S

N

V

I

D

S

E

E

L

L

R

Y

E

Q

L

L

T

L

G

N

Q

Q

P

P

L

L

T

D

D

E

E

S

T

L

Q

E

W

S

C

L

A

K

-

Q

A

A

A

V

R

S

Q

Q

I

P

V

L

R

F

D

E

G

G

R

-

A

I

R

E

M

W

V

I

A

I

L

V

T
x
S

L

L

G

G

G
x
A

R

Q

G

G

A

A

L

F

L

A

V

K

E

H

R

N

H

H

R

T

V

F

L

Y

R

R

A

V

P

N

A

I

L

P

P

T

V
x
I

K

S

M

V

V

L

S

N

A
x
P

I

V

G
|
G

A
x
S

G
|
G

D
|
D

S

S

F

T

L

V

A

A

A

G

L

I

V

T

W

S

A

A

L

I

N

L

R

N

G

H

A

E

G

N

T

D

G

H

E

D

A

L

F

L

R

K

Y

K

G

A

M
x
N

A

T

A

L

A
x
G

A
x
M

A

L

A

N

L

A

L

Q

S

E

A

A

G

Q

T

T

G

G

L

Y

C

V

H

N

K

L

E

N

D

N

V

-

E

-

R

-

F

-

Y

Y

R

D

D

D

M

L

P

F

S

N

H

Q

A

I

A

E

T

V

L

L

E

E

active site: G255 (= G253), S256 (≠ A254), G257 (= G255), D258 (= D256)
binding adenosine-5'-diphosphate: S226 (≠ T224), A229 (≠ G227), I248 (≠ V246), P253 (≠ A251), S256 (≠ A254), G257 (= G255), N282 (≠ M280), G285 (≠ A283), M286 (≠ A284)

2jg1A Structure of staphylococcus aureus d-tagatose-6-phosphate kinase with cofactor and substrate (see paper)
26% identity, 99% coverage: 4:315/315 of query aligns to 10:317/318 of 2jg1A

query
sites
2jg1A

I

I

L

L

T

T

F

L

T

T

P

L

N

N

P

P

S

S

I

V

D

D

V

I

F

S

T

Y

T

P

V

L

E

T

R

A

V

L

V

K

D

L

T

D

R

D

K

V

M

N

R

R

C

V

G

Q

A

E

E

V

R

S

R

K

D

T

P

A

G

G

G
x
K

G

G

V

L

N

N

V

V

A

T

R

R

V

V

I

L

Q

A

R

Q

L

V

G

G

G

E

D

P

C

V

T

L

A

A

V

S

V

G

M

F

A

I

G

G

Q

E

T

L

G

G

E

Q

M

F

L

I

R

A

Q

K

L

K

L

L

A

D

A

H

E

A

K

D

V

I

P

K

T

H

A

A

C

F

V

Y

G

N

I

I

A

K

G

G

H

E

T

T

R

R

E

N

S

C

F

I

S

A

V

I

L

L

E

H

T

E

G

G

T

Q

G

Q

R

T

E

E

F

-

R

-

F

I

V

L

L

E

P

Q

G

G

P

P

V

E

I

I

T

-

G

-

N

-

D

D

W

N

Q

Q

R

E

C

A

H

A

D

G

A

F

L

I

A

K

A

H

F

F

D

E

-

Q

-

M

P

E

A

K

P

V

R

E

F

A

L

V

V

A

L

I

S

S

G

G

R

S

L

L

P

P

P

K

G

G

A

L

P

N

V

Q

D

D

G

Y

Y

Y

A

A

Q

Q

L

I

A

I

R

E

A

R

A

C

A

Q

A

N

R

K

G

G

T

V

Q

P

V

V

A

I

I

L

D

D

T

C

S

S

G

G

P

A

A

T

L

L

A

Q

A

T

A

V

L

L

E

E

A

N

G

P

V

Y

-

K

-

P

A

T

I

V

V

I

K

K

P

P

S
x
N

V

I

D

S

E

E

L

L

R

Y

E

Q

L

L

T

L

G

N

Q

Q

P

P

L

L

T

D

D

E

E

S

T

L

Q

E

W

S

C

L

A

K

-

Q

A

A

A

V

R

S

Q

Q

I

P

V

L

R

F

D

E

G

G

R

-

A

I

R

E

M

W

V

I

A

I

L

V

T
x
S

L

L

G
|
G

G

A

R

Q

G
|
G

A

A

L

F

L

A

V

K

E

H

R

N

H

H

R

T

V

F

L

Y

R

R

A

V

P

N

A

I

L

P

P

T

V
x
I

K

S

M
x
V

V

L

S

N

A

P

I

V

G
|
G

A
x
S

G
|
G

D
|
D

S

S

F
x
T

L

V

A

A

A

G

L

I

V

T

W

S

A

A

L

I

N

L

R

N

G

H

A

E

G

N

T

D

G

H

E

D

A

L

F

L

R

K

Y

K

G

A

M
x
N

A

T

A

L

A
x
G

A
x
M

A

L

A

N

L

A

L

Q

S

E

A

A

G

Q

T

T

G

G

L

Y

C

V

H

N

K

L

E

N

D

N

V

-

E

-

R

-

F

-

Y

Y

R

D

D

D

M

L

P

F

S

N

H

Q

A

I

A

E

T

V

L

L

E

E

active site: G259 (= G253), S260 (≠ A254), G261 (= G255), D262 (= D256)
binding phosphoaminophosphonic acid-adenylate ester: K46 (≠ G40), N193 (≠ S187), S230 (≠ T224), G232 (= G226), G235 (= G229), I252 (≠ V246), V254 (≠ M248), G259 (= G253), S260 (≠ A254), G261 (= G255), D262 (= D256), T264 (≠ F258), N286 (≠ M280), G289 (≠ A283), M290 (≠ A284)

3ie7A The crystal structure of phosphofructokinase (lin2199) from listeria innocua in complex with atp at 1.6a
24% identity, 90% coverage: 4:288/315 of query aligns to 3:284/309 of 3ie7A

query
sites
3ie7A

I

I

L

Y

T

T

F

I

T

T

P

L

N

N

P

P

S

A

I

I

D

D

V

R

F

L

T

L

T

F

V

I

E

R

R

G

V

E

V

L

D

E

T

K

R

R

K

K

M

T

-

N

R

R

C

V

G

I

A

K

E

T

R

E

R

F

D

D

P

C

G

G

G

K

G

G

V

L

N

H

V

V

A

S

R

G

V

V

I

L

Q

S

R

K

L

F

G

G

G

I

D

K

C

N

T

E

A

A

V

L

V

G

M

I

A

A

G

G

G

S

Q

D

T

N

G

L

E

D

M

K

L

L

R

Y

Q

A

L

I

L

L

A

K

A

E

E

K

K

H

V

I

P

N

T

H

A

D

C

F

V

L

G

V

I

E

A

A

G

G

-

T

H

S

T

T

R

R

E

E

S

C

F

F

S

V

V

V

L

L

E

S

T

D

G

D

T

T

G

N

R

G

E

S

F

T

R

M

F

I

V

P

L

E

P

A

G

G

P

F

V

T

I

V

T

S

G

Q

N

T

D

N

W

K

Q

D

R

N

C

L

H

L

D

K

A

Q

L

I

A

A

A

K

F

K

D

V

P

K

A

K

P

E

R

D

F

M

L

V

V

V

L

I

S

A

G

G

R

S

L

P

P

P

G

H

A

Y

P

T

V

L

D

S

G

D

Y

F

A

K

Q

E

L

L

A

L

R

R

A

T

A

V

A

K

A

A

R

T

G

G

T

A

Q

F

V

L

A

G

I

C

D

D

T

N

S

S

G

G

P

E

A

Y

L

L

A

N

A

L

A

A

L

V

E

E

A

M

G

G

V

V

A

D

I

F

V

I

K

K

P

P

S
x
N

V

E

D

D

E

E

L

V

R

I

E

A

L

I

T

-

G

-

Q

-

P

-

L

L

T

D

D

E

E

K

T

T

Q

N

W

S

C

L

A

E

A

N

R

I

Q

R

I

T

V

L

R

A

D

E

G

K

R

I

A

P

R

Y

M

L

V

V

A

-

L

V

T
x
S

L

L

G
|
G

G
x
A

R

K

G
|
G

A

S

L

I

L

C

V

A

E

H

R

N

H

G

R

K

V

L

L

Y

R

Q

A

V

P

I

A

P

L

P

P

K

V
|
V

K

Q

M

E

V

R

S

N

A

D

I

T

G
|
G

A
|
A

G
|
G

D
|
D

S

V

F

F

L

V

A

G

A

A

L

F

V

I

W

A

A

G

L

L

N

A

R

M

G

N

A

M

G

P

T

I

G

T

E

E

A

T

F

L

R

K

Y

V

G

A

M

T

A

G

A

C

A
x
S

A

A

A

S

A

K

L
x
V

L

M

binding adenosine-5'-triphosphate: N188 (≠ S187), S220 (≠ T224), G222 (= G226), A223 (≠ G227), G225 (= G229), V242 (= V246), G249 (= G253), A250 (= A254), G251 (= G255), D252 (= D256), S279 (≠ A283), V283 (≠ L287)

2ajrA Crystal structure of possible 1-phosphofructokinase (ec 2.7.1.56) (tm0828) from thermotoga maritima at 2.46 a resolution
26% identity, 90% coverage: 4:286/315 of query aligns to 3:293/320 of 2ajrA

query
sites
2ajrA

I

V

L

L

T

T

F

V

T

T

P

L

N

N

P

P

S

A

I

L

D

D

V

R

F

E

T

I

T

F

V

I

E

E

R

D

V

F

V

Q

D

V

T

N

R

R

K

L

M

Y

R

R

C

I

G

N

-

D

-

L

-

S

A

K

E

T

R

Q

R

M

D

S

P

P

G

G

G

K

G

G

V

I

N

N

V

V

A

S

R

I

V

A

I

L

Q

S

R

K

L

L

G

G

G

V

D

P

C

S

T

V

A

A

V

T

V

G

M

F

A

V

G

G

Q

Y

T

M

G

G

E

K

M

I

L

L

R

V

Q

E

L

E

L

L

-

R

-

K

A

I

A

S

E

K

K

L

V

I

P

T

T

T

A

N

C

F

V

V

G

Y

I

V

A

E

G

G

H

E

T

T

R

R

E

E

S

N

F

I

S

E

V

I

L

I

E

D

T

E

G

K

T

N

G

K

R

T

E

I

F

T

R

A

F

I

V

N

L

F

P

P

G

G

P

P

V

D

I

V

T

T

G

D

N

M

D

D

-

V

-

N

-

H

-

F

W

L

Q

R

R

R

C

Y

H

K

D

M

A

T

L

L

A

S

A

K

F

V

D

D

P

C

A

-

P

-

R

-

F

-

L

V

V

V

L

I

S

S

G

G

R

S

L

I

P

P

G

G

A

V

P

N

V

E

D

G

G

I

Y

C

A

N

Q

E

L

L

A

V

R

R

A

L

A

A

A

R

A

E

R

R

G

G

T

V

Q

F

V

V

A

F

I

V

D

E

T

Q

S

T

G

-

P

P

A

R

L

L

A

L

A

E

A

R

L

I

E

Y

A

E

G

G

V

P

A

E

-

F

-

P

-

N

I

V

V

V

K

K

P

P

S

-

V

-

D

-

E

D

L

L

R

R

-

G

-

N

-

H

-

A

E

S

L

F

T

L

G

G

Q

V

P

D

L

L

T

K

D

T

E

F

T

D

Q

D

W

Y

C

V

A

K

A

L

A

A

R

E

Q

K

I

L

V

A

R

E

D

K

G

S

R

Q

A

V

R

S

M

V

V

V

A

S

L

Y

T

E

L

V

G

K

G

N

R

D

G

I

A

V

L

A

L

T

V

R

E

E

R

G

H

V

R

W

V

L

L

I

R

R

A

S

P

K

A

E

L

-

P

E

V

I

K

D

M

T

V

S

S
x
H

A

L

I

L

G

G

A

A

G

G

D

D

S

A

F

Y

L

V

A

A

A

G

L

M

V

V

W

Y

A

Y

-

F

L

I

N

K

R

H

G

G

A

A

G

N

T

F

G

L

E

E

A

M

F

A

R

K

Y

F

G

G

M

F

A

A

A

S

A

A

A

L

A

A

binding magnesium ion: H256 (≠ S250)

Sites not aligning to the query:

binding magnesium ion: 296, 298

3julA Crystal structure of listeria innocua d-tagatose-6-phosphate kinase bound with substrate
24% identity, 90% coverage: 4:288/315 of query aligns to 3:277/298 of 3julA

query
sites
3julA

I

I

L

Y

T

T

F

I

T

T

P

L

N

N

P

P

S

A

I

I

D
|
D

V

R

F

L

T

L

T

F

V

I

E

R

R

G

V

E

V

L

D

E

T

K

R

R

K

K

M

T

-

N

R

R

C

V

G

I

A

K

E

T

R

E

R

F

D

D

P

C

G

G

G
x
K

G

G

V

L

N
x
H

V

V

A

S

R

G

V

V

I

L

Q

S

R

K

L

F

G

G

G

I

D

K

C

N

T

E

A

A

V

L

V

G

M

I

A

A

G

G

G

S

Q

D

T

N

G

L

E

D

M

K

L

L

R

Y

Q

A

L

I

L

L

A

K

A

E

E

K

K

H

V

I

P

N

T

H

A

D

C

F

V

L

G

V

I

E

A

A

G

G

-

T

H

S

T

T

R
|
R

E

E

S

C

F

F

S

V

V

V

L

L

E

S

T

D

G

D

T

T

G

N

R

G

E

S

F

T

R
x
M

F

I

V

P

L

E

P

A

G

G

P

F

V

T

I

V

T

S

G

Q

N

T

D

N

W

K

Q

D

R

N

C

L

H

L

D

K

A

Q

L

I

A

A

A

K

F

K

D

V

P

K

A

K

P

E

R

D

F

M

L

V

V

V

L

I

S

A

G
|
G

R

S

L

P

P

P

G

H

A

Y

P

T

V

L

D

S

G

D

Y

F

A

K

Q

E

L

L

A

L

R

R

A

T

A

V

A

K

A

A

R

T

G

G

T

A

Q

F

V

L

A

G

I

C

D

D

T

N

S

S

G

G

P

E

A

Y

L

L

A

N

A

L

A

A

L

V

E

E

A

M

G

G

V

V

A

D

I

F

V

I

K

K

P

P

S

N

V

E

D

D

E

E

L

V

R

I

E

A

L

I

T

-

G

-

Q

-

P

-

L

L

T

D

D

E

E

K

T

T

Q

N

W

S

C

L

A

E

A

N

R

I

Q

R

I

T

V

L

R

A

D

E

G

K

R

I

A

P

R

Y

M

L

V

V

A

-

L

V

T

S

L

L

G

G

G

A

R

K

G

G

A

S

L

I

L

C

V

A

E

H

R

N

H

G

R

K

V

L

L

Y

R

Q

A

-

P

-

A

-

L

-

P

-

V

-

K

-

M

V

V

I

S

P

A

P

I

T

G

G

A

A

G

G

D
|
D

S

V

F

F

L

V

A

G

A

A

L

F

V

I

W

A

A

G

L

L

N

A

R

M

G

N

A

M

G

P

T

I

G

T

E

E

A

T

F

L

R

K

Y

V

G

A

M

T

A

G

A

C

A

S

A

A

A

S

A

K

L

V

L

M

binding 6-O-phosphono-beta-D-tagatofuranose: D13 (= D14), K40 (≠ G40), H43 (≠ N43), R91 (= R90), M106 (≠ R105), G140 (= G139), D245 (= D256)

Q8ZKR2 Aminoimidazole riboside kinase; AIRs kinase; EC 2.7.1.223 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
27% identity, 81% coverage: 15:268/315 of query aligns to 7:264/319 of Q8ZKR2

query
sites
Q8ZKR2

V

V

F

W

T

V

T

I

V

G

E

D

R

A

V

S

V

V

D
|
D

T

L

R

V

K

P

M

E

R

K

C

Q

G

N

A

S

E

Y

R

L

R

K

D

C

P

P

G

G

G
|
G

G

A

G

S

V

A

N

N

V

V

A

G

R

V

V

C

I

V

Q

A

R

R

L

L

G

G

D

E

C

C

T

G

A

F

V

I

-

G

V

C

M

L

A

G

G

D</