SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_047215054.1 NCBI__GCF_001931675.1:WP_047215054.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q88JH0 Quinoprotein alcohol dehydrogenase PedH; Lanthanide-dependent pyrroloquinoline quinone-dependent alcohol dehydrogenase; Lanthanide-dependent PQQ-ADH; EC 1.1.2.- from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
40% identity, 97% coverage: 18:574/575 of query aligns to 9:585/595 of Q88JH0

query
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Q88JH0

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Q87 (≠ E95) binding pyrroloquinoline quinone
C131 (= C141) modified: Disulfide link with 132
C132 (= C142) modified: Disulfide link with 131
R137 (= R147) binding pyrroloquinoline quinone
S181 (≠ T191) binding pyrroloquinoline quinone
G197 (= G207) binding pyrroloquinoline quinone
G198 (= G208) binding pyrroloquinoline quinone
E199 (= E209) binding Pr(3+)
W263 (= W257) binding pyrroloquinoline quinone
N281 (= N275) binding Pr(3+)
D323 (= D317) binding Pr(3+)
D325 (= D319) binding Pr(3+)
R350 (= R344) binding pyrroloquinoline quinone
F412 (= F399) mutation to I: In contrast to wild-type, this mutant is able to oxidize 5-(hydroxymethyl)furoic acid (HMFA) into 5-formylfuroic acid (FFA); when associated with Ser-561 or Gln-561.; mutation to V: High decrease in affinity for ethanol, and in contrast to wild-type, this mutant is able to oxidize 5-(hydroxymethyl)furoic acid (HMFA) into 5-formylfuroic acid (FFA) and can also oxidize 5-(hydroxymethyl)furfural (HMF) and 5-formylfurfural (FFF); when associated with Ala-561.
N417 (= N404) binding pyrroloquinoline quinone
W493 (≠ N481) binding pyrroloquinoline quinone
A557 (= A546) binding pyrroloquinoline quinone
W561 (= W550) mutation to A: High decrease in affinity for ethanol, and in contrast to wild-type, this mutant is able to oxidize 5-(hydroxymethyl)furoic acid (HMFA) into 5-formylfuroic acid (FFA) and can also oxidize 5-(hydroxymethyl)furfural (HMF) and 5-formylfurfural (FFF); when associated with Val-412.; mutation W->S,Q: In contrast to wild-type, this mutant is able to oxidize 5-(hydroxymethyl)furoic acid (HMFA) into 5-formylfuroic acid (FFA); when associated with Ile-412.

6zcvA Crystal structure of lanthanide-dependent alcohol dehydrogenase pedh from pseudomonas putida kt2440
42% identity, 89% coverage: 61:574/575 of query aligns to 26:558/562 of 6zcvA

query
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6zcvA

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active site: E172 (= E209), N254 (= N275), D296 (= D317)
binding calcium ion: N161 (≠ K198), K163 (≠ S200), P278 (= P299), D279 (≠ A300)
binding pyrroloquinoline quinone: Q60 (≠ E95), C104 (= C141), C105 (= C142), I108 (≠ V145), R110 (= R147), S154 (≠ T191), G170 (= G207), G171 (= G208), E172 (= E209), W236 (= W257), D298 (= D319), R323 (= R344), N390 (= N404), W466 (≠ N481), G529 (= G545), A530 (= A546)

C5AXV8 Lanthanide-dependent ethanol dehydrogenase; Lanthanide-dependent EtDH; Ln-dependent EtDH; Lanthanide-dependent formaldehyde dehydrogenase; PQQ-dependent ethanol dehydrogenase; EC 1.1.2.-; EC 1.2.2.- from Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1) (Methylobacterium extorquens) (see paper)
40% identity, 98% coverage: 14:574/575 of query aligns to 8:582/587 of C5AXV8

query
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C5AXV8

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A

Q

Q

S

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N

F

S

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P

P

L

I

K

K

Q

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I

L

N

N

T

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A

D

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N

A

I

K

K

D

G

L

L

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V

Q

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V

A

W

W

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S

Y

F

K

S

F

F

P

G

A

G

E

E

L

K

Q

Q

Q

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G

G

F

Q

E

E

A

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T

Q

P

A

I

L

V

V

N

K

G

D

N

G

Y

V

M

L

F

Y

V

V

T

T

T

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P

S

K

Y

D

S

H

R

V

L

Y

Y

A

A

F

V

D

D

A

A

K

R

T

T

G

G

K

E

E

K

L

K

W

W

T

E

Y

Y

D

N

P

A

K

R

L

L

T

P

S

E

L

G

S

I

F

L

K

-

T

-

A

P

C

C

D

D

V

V

N

N

R

R

G

G

V

A

A

A

L

L

Y

Y

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K

K

D

N

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V

Y

Y

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A

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L

L

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A

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L

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A

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L

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D

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V

W

W

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K

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L

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D

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F

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G

G

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Y

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Y

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T

L

A

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A

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L

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A

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L

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K

K

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A

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A

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H

-

M

-

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-

L

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N

-

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P

P

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M

-

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-

G

K

K

G

A

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N

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W

W

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P

N

G

G

D

M

T

Y

W

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K

H

F

A

G

G

G

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G

P

A

A

T

W

W

L

L

T

G

G

G

T

T

Y

Y

D

D

V

P

K

E

T

L

N

N

T

L

L

I

F

Y

W

F

G

G

V

T

G

G

N

N

P

P

G

G

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P

W

W

L

N

A

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D

A

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L

R

R

P

P

G

G

K

D

N

N

L

K

Y

W

S

S

D

A

S

S

L

R

L

L

A

A

L

I

D

N

P

P

A

D

N

N

G

G

D

E

L

I

K

V

W

W

H

G

F

F

Q

Q

Y

T

T

T

S

P

H

H

D

D

T

G

W

W

D

D

Y

Y

D
|
D

G

G

V

V

N

N

T

E

P

F

V

V

L

P

A

F

N

D

I

M

T

Q

Y

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K

D

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G

K

K

H

T

Y

V

E

K

A

A

I

G

I

A

H

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A

A

D

D

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N

N

G

G

Y

F

I

F

H

Y

A

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L

D

D

R

R

T

T

N

N

G

G

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A

L

F

I

L

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A

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P

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V

V

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A

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T

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-

W

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G

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G

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A

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F

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-

G

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S

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D

G

H

Y

I

G

G

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L

L

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A

A

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I

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N

A

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T

G

K

K

K

K

V

V

V

W

W

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E

H

Y

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N

K

K

L

A

P

P

W

L

N

W

G

A

G

G

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V

A

L

S

T

T

T

A

A

G

G

G

N

L

L

V

V

F

F

S

T

G

G

S

T

L

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N

E

G

G

H

F

L

L

Y

K

A

A

F

F

D

D

Q

A

K

K

N

T

G

G

K

E

V

E

L

V

W

W

E

K

S

F

P

-

Q

Q

L

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A

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S

T

G

G

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V

I

V

A

A

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P

P

S

I

V

T

F

W

E

E

V

Q

D

D

G

G

T

E

E

Q

Y

W

V

I

A

G

V

V

L

A

A

A

G

G

Y

W

G

G

A

A

N

V

P

P

I

L

W

W

G

G

P

E

M

V

A

A

K

K

I

T

A

T

E

A

H

G

V

I

P

N

R

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A

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A

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L

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P

K

K

D319 (= D319) mutation to S: Loss of efficient ethanol oxidation with La(3+).

Q9Z4J7 Quinoprotein ethanol dehydrogenase; QEDH; Quinoprotein alcohol dehydrogenase (cytochrome c); Quinoprotein alcohol dehydrogenase (cytochrome c550); EC 1.1.2.8 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 4 papers)
39% identity, 90% coverage: 53:572/575 of query aligns to 53:613/623 of Q9Z4J7

query
sites
Q9Z4J7

L

L

T

Q

Y

Y

Y

G

R

M

S

G

Y

T

T

H

G

A

Q

Q

S

R

N

W

S

S

P

P

L

L

K

K

Q

Q

I

V

N

N

T

A

A

D

T

N

A

V

K

F

D

K

L

L

Q

T

Q

P

V

A

W

W

S

S

Y

Y

K

S

F

F

P

G

A

D

E

E

L

K

Q

Q

Q

R

G

G

F

Q

E
|
E

A

S

T

Q

P

A

I

I

V

V

N

S

G

D

N

G

Y

V

M

I

F

Y

V

V

T

T

T

A

P

S

K

Y

D

S

H

R

V

L

Y

F

A

A

F

L

D

D

A

A

K

K

T

T

G

G

K

K

E

R

L

L

W

W

T

T

Y

Y

D

N

P

H

K

R

L

L

T

P

S

D

L

D

S

I

F

-

K

-

T

R

A

P

C
|
C

D

D

V

V

N

N

R
|
R

G

G

V

A

A

A

L

I

Y

Y

G

G

K

D

N

K

V

V

Y

F

V

F

A

G

M

T

L

L

S

D

G

A

Q

S

V

V

A

A

L

L

D

N

A

K

Q

N

S

T

G

G

K

K

V

V

W

W

E

K

K

K

Q

K

L

F

F

A

E

D

P

H

G

G

I

A

G

G

Y

Y

A

T

F

M

T
|
T

L

G

A

A

P

P

L

T

A

I

L

V

K

K

D

D

S

G

-

K

-

T

-

G

-

K

-

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-

L

I

L

I

I

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x
H

G
|
G

S
|
S

S

S

G

G

G

D

E
|
E

Y

F

G

G

A

V

R

V

G

G

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R

I

L

A

F

A

A

L

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N

D

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P

A

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N

T

G

G

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E

I

E

Q

I

W

W

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M

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W

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F

V

V

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A

G

-

H

-

M

-

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-

L

-

N

-

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-

D

-

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-

T

-

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-

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G

-

D

-

V

-

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-

A

-

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-

S

-

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P

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N

D

E

D

K

R

G

N

G

S

N

P

T

T

W

G

P

K

N

V

G

E

M

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Y

W

Q

S

H

H

A

G

G

G

S

G

P

A

A

P

W

W

L

Q

T

S

G

A

T

S

Y

F

D

D

V

A

K

E

T

T

N

N

T

T

L

I

F

I

W

V

G

G

V

A

G

G

N
|
N

P

P

G

G

P

P

W

W

L

N

A

T

D

W

M

A

R

R

P

T

G

A

K

K

-

G

-

N

-

P

-

H

-

D

-

Y

-

D

N

S

L

L

Y

Y

S

T

D

S

S

G

L

Q

L

V

A

G

L

V

D

D

P

P

A

S

N

S

G

G

D

E

L

V

K

K

W

W

H

F

F

Y

Q

Q

Y

H

T

T

S

P

H

N

D

D

T

A

W

W

D
|
D

Y

F

D

S

G

G

V

N

N

N

T

E

P

L

V

V

L

L

A

F

N

D

I

Y

T

K

Y

A

K

K

-

D

G

G

K

K

H

I

Y

V

E

K

A

A

I

T

I

A

H

H

A

A

D

D

R
|
R

N

N

G

G

Y

F

I

F

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Y

A

V

I

V

D

D

R

R

T

S

N

N

G

G

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K

L

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I

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Y

N

A

A

K

F

P

P

F

F

V

V

K

D

A

N

T

I

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T

V

W

T

A

G

S

Y

H

T

I

-

D

-

L

A

K

A

T

G

G

V

R

P

P

I

V

Q

E

N

R

Q

E

K

G

H

Q

Y

R

P

P

-

P

-

L

-

P

-

E

-

P

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G

-

Q

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K

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H

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G

T

K

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A

I

V

E

E

T

V

C

S

P

P

S

P

F

F

L

L

G

G

K

K

N

N

W

W

W

N

S

P

V

M

S

A

Y

Y

D

S

P

Q

T

D

T

T

H

G

L

L

A

F

F

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A

L

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H

H

A

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A

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G

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E

M

G

G

F

F

E

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P

-

E

R

P

M

G

Y

S

D

K

D

G

H

Y

V

G

G

E

S

L

L

Q

R

A

A

I

M

D

D

V

P

N

V

T

S

G

G

K

K

K

V

V

V

W

W

S

E

H

H

W

K

S

E

K

H

L

L

P

P

W

L

N
x
W

G

A

G

G

V

V

A

L

S

A

T

T

A

A

G

G

G

N

L

L

V

V

F

F

S

T

G

G

S

T

L

G

N

D

G

G

H

Y

L

F

Y

K

A

A

F

F

D

D

Q

A

K

K

N

S

G

G

K

K

V

E

L

L

W

W

E

K

S

F

P

-

Q

Q

L

T

A

G

S

S

G

G

V

I

I

V

A

S

Q

P

P

P

S

I

V

T

F

W

E

E

V

Q

D

D

G

G

T

E

E

Q

Y

Y

V

L

A

G

V

V

L

T

A

V

G

G

Y

Y

G

G

A
|
A

N

V

P

P

I

L

W

W

G

G

P

D

M

M

A

A

K

D

I

L

A

T

E

R

H

P

V

V

P

A

R

Q

G

G

T

S

L

F

Y

W

V

V

F

F

A

K

L

L

E95 (= E95) binding pyrroloquinoline quinone
C139 (= C141) modified: Disulfide link with 140
CC 139:140 (= CC 141:142) mutation to AA: 15-fold decrease in catalytic activity with the natural electron acceptor cytochrome c550. Does not affect, or even increases, catalytic activity with artificial electron acceptors. Shows high decreased affinity for primary alcohols, while the affinity for the secondary alcohol 2-propanol is unaltered.
C140 (= C142) modified: Disulfide link with 139
R145 (= R147) binding pyrroloquinoline quinone
T189 (= T191) binding pyrroloquinoline quinone
HGS 207:209 (≠ VGS 203:205) binding pyrroloquinoline quinone
E213 (= E209) binding Ca(2+)
N300 (= N275) binding Ca(2+)
D350 (= D317) binding Ca(2+)
R378 (= R344) binding pyrroloquinoline quinone
W523 (≠ N481) binding pyrroloquinoline quinone
A587 (= A546) binding pyrroloquinoline quinone

Sites not aligning to the query:

1:34 signal peptide
45 binding Ca(2+)
48 binding Ca(2+)
51 binding Ca(2+)

1flgA Crystal structure of the quinoprotein ethanol dehydrogenase from pseudomonas aeruginosa (see paper)
39% identity, 90% coverage: 53:572/575 of query aligns to 19:579/582 of 1flgA

query
sites
1flgA

L

L

T

Q

Y

Y

Y

G

R

M

S

G

Y

T

T

H

G

A

Q

Q

S

R

N

W

S

S

P

P

L

L

K

K

Q

Q

I

V

N

N

T

A

A

D

T

N

A

V

K

F

D

K

L

L

Q

T

Q

P

V

A

W

W

S

S

Y

Y

K

S

F

F

P

G

A

D

E

E

L

K

Q

Q

Q

R

G

G

F

Q

E
|
E

A

S

T

Q

P

A

I

I

V

V

N

S

G

D

N

G

Y

V

M

I

F

Y

V

V

T

T

T

A

P

S

K

Y

D

S

H

R

V

L

Y

F

A

A

F

L

D

D

A

A

K

K

T

T

G

G

K

K

E

R

L

L

W

W

T

T

Y

Y

D

N

P

H

K

R

L

L

T

P

S

D

L

D

S

I

F

-

K

-

T

R

A

P

C
|
C

D

D

V

V

N

N

R
|
R

G

G

V

A

A

A

L

I

Y

Y

G

G

K

D

N

K

V

V

Y

F

V

F

A

G

M

T

L

L

S

D

G

A

Q

S

V

V

A

A

L

L

D

N

A

K

Q

N

S

T

G

G

K

K

V

V

W

W

E

K

K

K

Q

K

L

F

F

A

E

D

P

H

G

G

I

A

G

G

Y

Y

A

T

F

M

T
|
T

L

G

A

A

P

P

L

T

A

I

L

V

K

K

D

D

S

G

-

K

-

T

-

G

-

K

-

V

-

L

I

L

I

I

V

H

G

G

S

S

S
|
S

G
|
G

G
x
D

E
|
E

Y

F

G

G

A

V

R

V

G

G

F

R

I

L

A

F

A

A

L

R

N

D

P

P

A

D

N

T

G

G

E

E

I

E

Q

I

W

W

K

M

R

R

W

P

T

F

V

V

P

E

A

G

-

H

-

M

-

G

-

R

-

L

-

N

-

G

-

K

-

D

-

S

-

T

-

V

-

T

-

G

-

D

-

V

-

K

-

A

-

P

-

S

-

W

P

P

N

D

E

D

K

R

G

N

G

S

N

P

T

T

W

G

P

K

N

V

G

E

M

S

Y

W

Q

S

H

H

A

G

G

G

S

G

P

A

A

P

W
|
W

L

Q

T

S

G

A

T

S

Y

F

D

D

V

A

K

E

T

T

N

N

T

T

L

I

F

I

W

V

G

G

V

A

G

G

N
|
N

P

P

G

G

P

P

W

W

L

N

A

T

D

W

M

A

R

R

P

T

G

A

K

K

-

G

-

N

-

P

-

H

-

D

-

Y

-

D

N

S

L

L

Y

Y

S

T

D

S

S

G

L

Q

L

V

A

G

L

V

D

D

P

P

A

S

N

S

G

G

D

E

L

V

K

K

W

W

H

F

F

Y

Q

Q

Y

H

T

T

S

P

H

N

D

D

T

A

W

W

D
|
D

Y

F

D

S

G

G

V

N

N

N

T

E

P

L

V

V

L

L

A

F

N

D

I

Y

T

K

Y

A

K

K

-

D

G

G

K

K

H

I

Y

V

E

K

A

A

I

T

I

A

H

H

A

A

D

D

R
|
R

N

N

G

G

Y

F

I

F

H

Y

A

V

I

V

D

D

R

R

T

S

N

N

G

G

K

K

L

L

I

Q

Y

N

A

A

K

F

P

P

F

F

V

V

K

D

A

N

T

I

S

T

V

W

T

A

G

S

Y

H

T

I

-

D

-

L

A

K

A

T

G

G

V

R

P

P

I

V

Q

E

N

R

Q

E

K

G

H

Q

Y

R

P

P

-

P

-

L

-

P

-

E

-

P

-

G

-

Q

K

K

V

H

G

G

T

K

T

A

I

V

E

E

T

V

C

S

P

P

S

P

F

F

L

L

G

G

K

K

N
|
N

W
|
W

W

N

S

P

V

M

S

A

Y

Y

D

S

P

Q

T

D

T

T

H

G

L

L

A

F

F

Y

V

V

P

P

S

A

L

N

H

H

A

W

C

K

M

E

T

D

M

Y

S

W

G

T

K

E

A

E

V

V

T

S

Y

Y

M

T

Q

K

G

G

L

S

P

A

Y

Y

L

L

G

G

E

M

G

G

F

F

E

R

I

I

R

K

P

-

E

R

P

M

G

Y

S

D

K

D

G

H

Y

V

G

G

E

S

L

L

Q

R

A

A

I

M

D

D

V

P

N

V

T

S

G

G

K

K

K

V

V

V

W

W

S

E

H

H

W

K

S

E

K

H

L

L

P

P

W

L

N
x
W

G

A

G

G

V

V

A

L

S

A

T

T

A

A

G

G

G

N

L

L

V

V

F

F

S

T

G

G

S

T

L

G

N

D

G

G

H

Y

L

F

Y

K

A

A

F

F

D

D

Q

A

K

K

N

S

G

G

K

K

V

E

L

L

W

W

E

K

S

F

P

-

Q

Q

L

T

A

G

S

S

G

G

V

I

I

V

A

S

Q

P

P

P

S

I

V

T

F

W

E

E

V

Q

D

D

G

G

T

E

E

Q

Y

Y

V

L

A

G

V

V

L

T

A

V

G

G

Y

Y

G

G

G
|
G

A
|
A

N

V

P

P

I

L

W

W

G

G

P

D

M

M

A

A

K

D

I

L

A

T

E

R

H

P

V

V

P

A

R

Q

G

G

T

S

L

F

Y

W

V

V

F

F

A

K

L

L

active site: E179 (= E209), N266 (= N275), D316 (= D317)
binding calcium ion: E179 (= E209), N266 (= N275), D316 (= D317)
binding pyrroloquinoline quinone: E61 (= E95), C105 (= C141), C106 (= C142), R111 (= R147), T155 (= T191), S176 (= S206), G177 (= G207), D178 (≠ G208), W248 (= W257), R344 (= R344), N413 (= N404), W414 (= W405), W489 (≠ N481), G552 (= G545), A553 (= A546)

Sites not aligning to the query:

binding calcium ion: 11, 14, 17

Q46444 Quinohemoprotein alcohol dehydrogenase; QH-ADH; Alcohol dehydrogenase (azurin); PQQ-containing alcohol dehydrogenase; PQQ-dependent ADH; Quinohaemoprotein ethanol dehydrogenase type I; QH-EDHI; EC 1.1.9.1 from Comamonas testosteroni (Pseudomonas testosteroni) (see 3 papers)
38% identity, 92% coverage: 45:572/575 of query aligns to 53:596/708 of Q46444

query
sites
Q46444

S

N

A

A

Q

A

A

R

D

T

P

P

G

D

W

W

L

P

T

T

Y

I

Y

G

R

V

S

D

Y

Y

T

A

G

E

Q

T

S

R

N

Y

S

S

P

R

L

L

K

D

Q

Q

I

I

N

N

T

A

A

A

T

N

A

V

K

K

D

D

L

L

Q

G

Q

L

V

A

W

W

S

S

Y

Y

K

N

F

L

P

-

A

-

E

E

L

S

Q

T

Q

R

G

G

F

V

E
|
E

A

A

T

T

P

P

I

V

V

V

N

V

G

D

N

G

Y

I

M

M

F

Y

V

V

T

S

T

A

P

S

K

W

D

S

H

V

V

V

Y

H

A

A

F

I

D

D

A

T

K

R

T

T

G

G

K

N

E

R

L

I

W

W

T

T

Y

Y

D

D

P

P

K

Q

L

I

T

D

S

R

L

S

S

T

F

G

K

F

T

K

A

G

C
|
C

D

D

V

V

N

N

R
|
R

G

G

V

V

A

A

L

L

Y

W

G

K

K

G

N

K

V

V

Y

Y

V

V

A

G

M

A

L

W

S

D

G

G

Q

R

V

L

V

I

A

A

L

L

D

D

A

A

Q

A

S

T

G

G

K

K

V

E

V

V

W

W

E

H

K

Q

Q

N

L

T

F

F

E

E

P

G

G

Q

I

K

G

G

-

S

Y

L

A

T

F

I

T
|
T

L

G

A

A

P

P

L

R

A

V

L

F

K

K

D

G

S

K

I

V

I

I

V

I

G

G

S

K

S

R

G
|
G

G
x
A

E
|
E

Y

Y

G

G

A

V

R

R

G

G

F

Y

I

I

A

T

A

A

L

Y

N

D

P

A

A

E

N

T

G

G

E

E

I

R

Q

K

W

W

K

R

R

W

W

F

T

S

V

V

P

P

A

G

-

D

-

P

-

S

-

K

P

P

N

F

E

E

-

D

-

E

-

S

-

M

-

K

K

R

G

A

N

R

T

T

W

W

-

D

P

P

N

S

G

G

M

K

Y

W

Q

W

H

E

A

A

-

G

G

G

S

G

P
x
T

A

M

W

W

L

D

T

S

G

M

T

T

Y

F

D

D

V

A

K

E

T

L

N

N

T

T

L

M

F

Y

W

V

G

G

V

T

G

G

N
|
N

P

G

G

S

P

P

W

W

L

S

A

H

D

K

M

V

R

R

P

S

-

P

-

K

-

G

G

G

K

D

N

N

L

L

Y

Y

S

L

D

A

S

S

L

I

L

V

A

A

L

L

D

D

P

P

A

D

N

T

G

G

D

K

L

Y

K

K

W

W

H

H

F

Y

Q

Q

Y

E

T

T

S

P

H

G

D

D

T

N

W

W

D
|
D

Y

Y

D

T

G

S

V

T

N

Q

T

P

P

M

V

I

L

L

A

A

N

D

I

I

T

K

Y

I

K

A

G

G

K

K

H

P

Y

R

E

K

A

V

I

I

I

L

H

H

A

A

D

P

R
x
K

N

N

G

G

Y

F

I

F

H

F

A

V

I

L

D

D

R

R

T

T

N

N

G

G

K

K

L

F

I

I

Y

S

A

A

K

K

P

N

F

F

V

V

K

P

A

V

T

N

S

W

V

A

T

S

G

G

Y

Y

T

D

A

K

A

H

G

G

V

K

P

P

I

I

-

G

-

I

-

A

Q

A

N

R

Q

D

K

G

H

S

Y

K

P

P

K

Q

V

-

G

-

T

-

I

-

E

D

T

A

C

V

P

P

S

G

F

P

L

Y

G

G

G

A

K

H

N
|
N

W
|
W

W

H

S

P

V

M

S

S

Y

F

D

N

P

P

T

Q

T

T

H

G

L

L

A

V

F

Y

V

L

P

P

S

A

L

Q

H

N

A

V

C

P

M

V

T

N

-

L

M

M

S

D

G

D

K

K

A

K

V

W

T

E

Y

F

M

N

Q

Q

-

A

-

G

-

P

G

G

L

K

P

P

Y

Q

L

S

G

G

E

T

G

G

-

W

-

N

-

T

-

A

-

K

-

F

F

F

E

N

I

A

R

E

P

P

E

-

P

P

G

K

S

S

K

K

G

P

Y

F

G

G

E

R

L

L

Q

L

A

A

I

W

D

D

V

P

N

V

T

A

G

Q

K

K

K

A

V

A

W

W

S

S

H

V

W

E

S

H

K

V

L

S

P

P

W

W

N

N

G

G

V

T

A

L

S

T

T

T

A

A

G

G

G

N

L

V

V

V

F

F

S

Q

G

G

S

T

L

A

N

D

G

G

H

R

L

L

Y

V

A

A

F

Y

D

H

Q

A

K

A

N

T

G

G

K

E

V

K

L

L

W

W

E

E

S

A

P

P

Q

T

L

-

A

G

S

T

G

G

V

V

I

V

A

A

Q

A

P

P

S

S

V

T

F

Y

E

M

V

V

D

D

G

G

T

R

E

Q

Y

Y

V

V

A

S

V

V

L

A

A

V

G

G

Y

W

G

G

A

-

N

-

P

-

I
x
V

W

Y

G

G

G

-

P

-

M

L

A

A

K

A

I

R

A

A

E

T

H

E

V

R

P

Q

R

G

G

P

G

G

T

T

L

V

Y

Y

V

T

F

F

A

V

L

V

E101 (= E95) binding pyrroloquinoline quinone
C147 (= C141) modified: Disulfide link with 148
C148 (= C142) modified: Disulfide link with 147
R153 (= R147) binding pyrroloquinoline quinone
T198 (= T191) binding pyrroloquinoline quinone
GA 214:215 (≠ GG 207:208) binding pyrroloquinoline quinone
E216 (= E209) binding Ca(2+)
T274 (≠ P255) binding pyrroloquinoline quinone
N294 (= N275) binding Ca(2+)
D339 (= D317) binding Ca(2+)
K366 (≠ R344) binding pyrroloquinoline quinone
NW 425:426 (= NW 404:405) binding pyrroloquinoline quinone
V575 (≠ I549) binding pyrroloquinoline quinone

Sites not aligning to the query:

1:31 signal peptide
635 binding covalent
638 binding covalent
639 binding axial binding residue
678 binding axial binding residue

1kb0A Crystal structure of quinohemoprotein alcohol dehydrogenase from comamonas testosteroni (see paper)
38% identity, 92% coverage: 45:572/575 of query aligns to 22:565/670 of 1kb0A

query
sites
1kb0A

S

N

A

A

Q

A

A

R

D

T

P

P

G

D

W

W

L

P

T

T

Y

I

Y

G

R

V

S

D

Y

Y

T

A

G

E

Q

T

S

R

N

Y

S

S

P

R

L

L

K

D

Q

Q

I

I

N

N

T

A

A

A

T

N

A

V

K

K

D

D

L

L

Q

G

Q

L

V

A

W

W

S

S

Y

Y

K

N

F

L

P

-

A

-

E

E

L

S

Q

T

Q

R

G

G

F

V

E
|
E

A

A

T

T

P

P

I

V

V

V

N

V

G

D

N

G

Y

I

M

M

F

Y

V

V

T

S

T

A

P

S

K

W

D

S

H

V

V

V

Y

H

A

A

F

I

D

D

A

T

K

R

T

T

G

G

K

N

E

R

L

I

W

W

T

T

Y

Y

D

D

P

P

K

Q

L

I

T

D

S

R

L

S

S

T

F

G

K

F

T

K

A

G

C
|
C

D

D

V

V

N

N

R
|
R

G

G

V

V

A

A

L

L

Y

W

G

K

K

G

N

K

V

V

Y

Y

V

V

A

G

M

A

L

W

S

D

G

G

Q

R

V

L

V

I

A

A

L

L

D

D

A

A

Q

A

S

T

G

G

K

K

V

E

V

V

W

W

E

H

K

Q

Q

N

L

T

F

F

E

E

P

G

G

Q

I

K

G

G

-

S

Y

L

A

T

F

I

T
|
T

L

G

A

A

P

P

L

R

A

V

L

F

K

K

D

G

S

K

I

V

I

I

V

I

G

G

S

N

S
x
G

G
|
G

G
x
A

E
|
E

Y

Y

G

G

A

V

R

R

G

G

F

Y

I

I

A

T

A

A

L

Y

N

D

P

A

A

E

N

T

G

G

E

E

I

R

Q

K

W

W

K

R

R

W

W

F

T

S

V

V

P

P

A

G

-

D

-

P

-

S

-

K

P

P

N

F

E

E

-

D

-

E

-

S

-

M

-

K

K

R

G

A

N

R

T

T

W

W

-

D

P

P

N

S

G

G

M

K

Y

W

Q

W

H

E

A

A

-

G

G

G

S

G

P
x
T

A

M

W
|
W

L

D

T

S

G

M

T

T

Y

F

D

D

V

A

K

E

T

L

N

N

T

T

L

M

F

Y

W

V

G

G

V

T

G

G

N
|
N

P

G

G

S

P

P

W

W

L

S

A

H

D

K

M

V

R

R

P

S

-

P

-

K

-

G

G

G

K

D

N

N

L

L

Y

Y

S

L

D

A

S

S

L

I

L

V

A

A

L

L

D

D

P

P

A

D

N

T

G

G

D

K

L

Y

K

K

W

W

H

H

F

Y

Q

Q

Y

E

T

T

S

P

H

G

D

D

T

N

W

W

D
|
D

Y

Y

D

T

G

S

V

T

N

Q

T

P

P

M

V

I

L

L

A

A

N

D

I

I

T

K

Y

I

K

A

G

G

K

K

H

P

Y

R

E

K

A

V

I

I

I

L

H

H

A

A

D

P

R
x
K

N

N

G

G

Y

F

I

F

H

F

A

V

I

L

D

D

R

R

T

T

N

N

G

G

K

K

L

F

I

I

Y

S

A

A

K

K

P

N

F

F

V

V

K

P

A

V

T

N

S

W

V

A

T

S

G

G

Y

Y

T

D

A

K

A

H

G

G

V

K

P

P

I

I

-

G

-

I

-

A

Q

A

N

R

Q

D

K

G

H

S

Y

K

P

P

K

Q

V

-

G

-

T

-

I

-

E

D

T

A

C

V

P

P

S

G

F
x
P

L

Y

G

G

G

A

K

H

N
|
N

W
|
W

W

H

S

P

V

M

S

S

Y

F

D

N

P

P

T

Q

T

T

H

G

L

L

A

V

F

Y

V

L

P

P

S

A

L

Q

H

N

A

V

C

P

M

V

T

N

-

L

M

M

S

D

G

D

K

K

A

K

V

W

T

E

Y

F

M

N

Q

Q

-

A

-

G

-

P

G

G

L

K

P

P

Y

Q

L

S

G

G

E

T

G

G

-

W

-

N

-

T

-

A

-

K

-

F

F

F

E

N

I

A

R

E

P

P

E

-

P

P

G

K

S

S

K

K

G

P

Y

F

G

G

E

R

L

L

Q

L

A

A

I

W

D

D

V

P

N

V

T

A

G

Q

K

K

K

A

V

A

W

W

S

S

H

V

W

E

S

H

K

V

L

S

P

P

W
|
W

N

N

G

G

V

T

A

L

S

T

T

T

A

A

G

G

G

N

L

V

V

V

F

F

S

Q

G

G

S

T

L

A

N

D

G

G

H

R

L

L

Y

V

A

A

F

Y

D

H

Q

A

K

A

N

T

G

G

K

E

V

K

L

L

W

W

E

E

S

A

P

P

Q

-

L

T

A

G

S

T

G

G

V

V

I

V

A

A

Q

A

P

P

S

S

V

T

F

Y

E

M

V

V

D

D

G

G

T

R

E

Q

Y

Y

V

V

A

S

V

V

L

A

A

V

G

G

Y

W

G

G

G
|
G

A

-

N

-

P

-

I
x
V

W

Y

G

G

G

-

P

-

M

L

A

A

K

A

I

R

A

A

E

T

H

E

V

R

P

Q

R

G

G

P

G

G

T

T

L

V

Y

Y

V

T

F

F

A

V

L

V

active site: E185 (= E209), N263 (= N275), D308 (= D317)
binding calcium ion: E185 (= E209), N263 (= N275), D308 (= D317)
binding pyrroloquinoline quinone: E70 (= E95), C116 (= C141), C117 (= C142), R122 (= R147), T167 (= T191), G182 (≠ S206), G183 (= G207), A184 (≠ G208), E185 (= E209), T243 (≠ P255), W245 (= W257), D308 (= D317), K335 (≠ R344), N394 (= N404), W395 (= W405), W479 (= W480), G543 (= G545), V544 (≠ I549)
binding tetrahydrofuran-2-carboxylic acid: C116 (= C141), C117 (= C142), E185 (= E209), D308 (= D317), P389 (≠ F399)

Sites not aligning to the query:

binding heme c: 598, 599, 602, 603, 617, 620, 631, 637, 640, 642, 643, 645

1kv9A Structure at 1.9 a resolution of a quinohemoprotein alcohol dehydrogenase from pseudomonas putida hk5 (see paper)
36% identity, 92% coverage: 45:572/575 of query aligns to 11:550/664 of 1kv9A

query
sites
1kv9A

S

T

A

E

Q

Q

A

A

D

G

P

G

G

E

W

W

L

L

T

S

Y

H

Y

G

R

R

S

T

Y

Y

T

A

G

E

Q

Q

S

R

N

F

S

S

P

P

L

L

K

K

Q

Q

I

I

N

D

T

A

A

S

T

N

A

V

K

R

D

S

L

L

Q

G

Q

L

V

A

W

W

S

Y

Y

M

K

D

F

L

P

-

A

-

E

D

L

N

Q

T

Q

R

G

G

F

L

E
|
E

A

A

T

T

P

P

I

L

V

F

N

H

G

D

N

G

Y

V

M

I

F

Y

V

T

T

S

T

M

P

S

K

W

D

S

H

R

V

V

Y

I

A

A

F

V

D

D

A

A

K

A

T

S

G

G

K

K

E

E

L

L

W

W

T

R

Y

Y

D

D

P

P

K

E

L

V

T

A

S

K

L

V

S

K

F
x
A

K
x
R

T

T

A

S

C
|
C

D

D

V

A

V

V

N

N

R
|
R

G

G

V

V

A

A

L

L

Y

W

G

G

K

D

N

K

V

V

Y

Y

V

V

A

G

M

T

L

L

S

D

G

G

Q

R

V

L

V

I

A

A

L

L

D

D

A

A

Q

K

S

T

G

G

K

K

V

A

V

I

W

W

E

S

K

Q

Q

Q

L

T

F

T

E

D

P

P

G

A

I

K

G

P

Y

Y

A

S

F

I

T
|
T

L

G

A

A

P

P

L

R

A

V

L

V

K

K

D

G

S

K

I

V

I

I

V

I

G

G

S

N

S
x
G

G

G

G
x
A

E
|
E

Y

Y

G

G

A

V

R

R

G

G

F

F

I

V

A

S

A

A

L

Y

N

D

P

A

A

D

N

T

G

G

E

K

I

L

Q

A

W

W

K

R

R

F

W

Y

T

T

V

V

P

P

A

G

-

D

-

P

-

A

-

L

P

P

N

Y

E

E

-

H

-

P

-

E

-

L

-

R

K

E

G

A

N

K

T

T

W

W

P

Q

N

G

G

D

M

Q

Y

Y

Q

W

H

K

-

L

-

G

A

G

G

G

S

G

P
x
T

A

V

W
|
W

L

D

T

S

G

M

T

A

Y

Y

D

D

V

P

K

E

T

L

N

D

T

L

L

L

F

Y

W

V

G

G

V

T

G

G

N
|
N

P

G

G

S

P

P

W

W

L

N

A

R

D

E

M

V

R

R

P

S

-

P

-

G

G

G

K

D

N

N

L

L

Y

Y

S

L

D

S

S

S

L

I

L

L

A

A

L

I

D

R

P

P

A

D

N

T

G

G

D

K

L

L

K

A

W

W

H

H

F

Y

Q

Q

Y

V

T

T

S

P

H

G

D

D

T

S

W

W

D
|
D

Y

F

D

T

G

A

V

T

N

Q

T

Q

P

I

V

T

L

L

A

A

N

E

I

L

T

N

Y

I

K

D

G

G

K

K

H

P

Y

R

E

K

A

V

I

L

I

M

H

Q

A

A

D

P

R
x
K

N

N

G

G

Y

F

I

F

H

Y

A

V

I

L

D

D

R

R

T

T

N

N

G

G

K

K

L

L

I

I

Y

S

A

A

K

E

P

K

F

F

V

G

K

K

A

V

T

T

S

W

V

A

T

E

G

K

Y

V

T

D

-

L

A

A

A

T

G

G

V

R

P

P

I

V

Q

E

N

A

Q

P

K

G

-

V

H

R

Y

Y

P

E

K

K

V

-

G

-

T

E

T

P

I

I

E

V

T

M

C

W

P

P

S

S

F

P

L

F

G

G

G

A

K

H

N
|
N

W
|
W

W

H

S

S

V

M

S

S

Y

F

D

N

P

P

T

G

T

T

H

G

L

L

A

V

F

Y

V

I

P

P

-

Y

-

Q

S

E

L

V

H

P

A

G

C

V

M

Y

T

R

M

N

S

E

G

G

K

K

A

D

V

F

T

V

Y

T

M

R

Q

K

G

A

L

F

P

N

Y

T

L

A

G

A

E

G

G

F

F

A

E

D

I

A

R

T

P

D

E

V

P

P

G

A

S

A

K

V

G

V

Y

S

G

G

E

A

L

L

Q

L

A

A

I

W

D

D

V

P

N

V

T

K

G

Q

K

K

K

A

V

A

W

W

S

K

H

V

W

P

S

Y

K

P

L

T

P

H

W
|
W

N

N

G

G

V

T

A

L

S

S

T

T

A

A

G

G

G

N

L

L

V

V

F

F

S

Q

G

G

S

T

L

A

N

A

G

G

H

Q

L

M

Y

H

A

A

F

Y

D

S

Q

A

K

D

N

K

G

G

K

E

V

A

L

L

W

W

E

Q

S

F

P

-

Q

E

L

A

A

Q

S

S

G

G

V

I

I

V

A

A

Q

A

P

P

S

M

V

T

F

F

E

E

V

L

D

A

G

G

T

R

E

Q

Y

Y

V

V

A

A

V

I

L

M

A

A

G

G

Y

W

G

G

A
x
V

N

A

P

T

I

L

W

T

G

G

P

E

M

S

A

M

K

N

I

L

A

P

E

G

H

M

V

K

P

N

R

R

G

-

G

S

T

R

L

L

Y

L

V

V

F

F

A

A

L

L

active site: E173 (= E209), N250 (= N275), D295 (= D317)
binding acetone: E173 (= E209), D295 (= D317)
binding calcium ion: E173 (= E209), N250 (= N275), D295 (= D317)
binding heme c: A101 (≠ F137), R102 (≠ K138)
binding pyrroloquinoline quinone: E59 (= E95), C105 (= C141), C106 (= C142), R111 (= R147), T155 (= T191), G170 (≠ S206), A172 (≠ G208), E173 (= E209), T230 (≠ P255), W232 (= W257), K322 (≠ R344), N382 (= N404), W383 (= W405), W460 (= W480), V525 (≠ A546)

Sites not aligning to the query:

binding heme c: 590, 591, 594, 595, 605, 606, 608, 611, 615, 619, 623, 631, 633, 636

Q8GR64 Quinohemoprotein alcohol dehydrogenase ADH IIB; ADH IIB; Alcohol dehydrogenase (azurin); EC 1.1.9.1 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 3 papers)
36% identity, 92% coverage: 45:572/575 of query aligns to 33:572/690 of Q8GR64

query
sites
Q8GR64

S
x
T

A
x
E

Q
|
Q

A
|
A

D
x
G

P
x
G

G
x
E

W
|
W

L
|
L

T
x
S

Y
x
H

Y
x</