SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_047216038.1 NCBI__GCF_001931675.1:WP_047216038.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

2ordA Crystal structure of acetylornithine aminotransferase (ec 2.6.1.11) (acoat) (tm1785) from thermotoga maritima at 1.40 a resolution
44% identity, 96% coverage: 11:395/399 of query aligns to 11:390/393 of 2ordA

query
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2ordA

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active site: F134 (= F138), E186 (= E190), D219 (= D223), Q222 (= Q226), K248 (= K252), T276 (= T280), R367 (= R372)
binding pyridoxal-5'-phosphate: G102 (= G102), T103 (≠ A103), F134 (= F138), H135 (= H139), E186 (= E190), D219 (= D223), V221 (= V225), Q222 (= Q226), K248 (= K252)

Q9X2A5 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)
44% identity, 96% coverage: 11:395/399 of query aligns to 3:382/385 of Q9X2A5

query
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Q9X2A5

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GT 94:95 (≠ GA 102:103) binding pyridoxal 5'-phosphate
T268 (= T280) binding pyridoxal 5'-phosphate

4addA Structural and functional study of succinyl-ornithine transaminase from e. Coli (see paper)
46% identity, 90% coverage: 25:384/399 of query aligns to 26:384/400 of 4addA

query
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4addA

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active site: F136 (= F138), E188 (= E190), D221 (= D223), Q224 (= Q226), K250 (= K252), T279 (= T280), R372 (= R372)
binding pyridoxal-5'-phosphate: G103 (= G102), A104 (= A103), F136 (= F138), H137 (= H139), D221 (= D223), V223 (= V225), K250 (= K252)
binding n~2~-(3-carboxypropanoyl)-l-ornithine: F136 (= F138), R139 (= R141)

Sites not aligning to the query:

binding n~2~-(3-carboxypropanoyl)-l-ornithine: 16

4adbB Structural and functional study of succinyl-ornithine transaminase from e. Coli (see paper)
46% identity, 90% coverage: 25:384/399 of query aligns to 26:384/401 of 4adbB

query
sites
4adbB

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F
|
F

H
|
H

G

G

R

R

T

T

L

L

A

F

T

T

M

V

S

S

A

A

S

G

G

G

K

Q

P

P

G

A

W

Y

D

S

K

Q

I

D

F

F

A

A

P

P

Q

L

V

P

P

A

G

D

F

I

P

R

K

H

A

A

E

A

L

Y

N

N

D

D

I

I

A

N

S

S

V

A

E

S

K

A

L

L

I

I

G

D

P

D

D

S

T

T

V

C

A

A

V

V

M

I

L

V

E
|
E

P

P

V

I

Q

Q

G

G

E

E

G

G

V

V

I

V

P

P

A

A

T

S

R

N

E

A

F

F

M

L

L

Q

Q

G

L

L

R

R

E

E

L

L

T

C

H

N

R

R

H

H

N

N

L

A

L

L

I

I

V

F

D
|
D

E

E

V
|
V

Q
|
Q

T

T

G

G

C

V

G

G

R

R

T

T

G

G

K

E

L

L

F

Y

A

A

Y

Y

E

M

L

H

S

Y

G

G

I

V

T

T

P

P

D

D

I

L

M

L

T

T

L

T

G

A

K
|
K

G

A

I

L

G

G

V

F

P

P

L

V

A

G

A

A

L

L

S

A

T

T

A

E

V

C

A

A

-

R

V

V

F

M

E

T

A

V

G

G

D

T

Q

H

G

G

G

T

T
|
T

Y

Y

N

G

G

G

N

N

P

P

L

L

M

A

T

S

A

A

V

V

G

A

I

G

S

K

V

V

I

L

E

E

Q

L

L

I

T

N

A

T

P

P

G

E

F

M

L

L

E

N

N

G

V

V

T

K

Q

Q

L

R

G

H

N

D

Y

W

L

F

R

V

E

E

Q

R

L

L

L

N

A

T

L

I

C

N

D

H

T

R

Y

Y

G

G

F

L

E

F

G

S

E

E

-

V

R

R

G

G

E

L

G

G

L

L

R

I

A

G

L

C

L

V

L

L

G

N

K

A

D

D

I

Y

G

A

P

G

Q

Q

I

A

V

K

E

Q

K

I

A

S

R

Q

N

E

L

A

S

A

P

K

D

A

G

G

L

V

L

M

V

V

N

L

A

I

A

A

R

G

P

G

N

N

L

V

R
|
R

F

F

M

A

P

P

A

A

L

L

N

N

V

V

T

S

R

E

E

E

I

V

active site: F136 (= F138), E188 (= E190), D221 (= D223), Q224 (= Q226), K250 (= K252), T279 (= T280), R372 (= R372)
binding pyridoxal-5'-phosphate: S102 (= S101), G103 (= G102), A104 (= A103), F136 (= F138), H137 (= H139), D221 (= D223), V223 (= V225), Q224 (= Q226), K250 (= K252)

O66442 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Aquifex aeolicus (strain VF5)
41% identity, 96% coverage: 11:395/399 of query aligns to 1:376/376 of O66442

query
sites
O66442

L

M

M

T

Y

Y

I

L

T

M

N

N

-

N

-

Y

-

A

R

R

P

L

E

P

T

V

V

K

F

F

T

V

H

R

G

G

K

K

G

G

S

V

W

Y

I

L

F

Y

D

D

H

E

T

E

G

G

K

K

R

E

Y

Y

L

L

D

D

F

F

I

V

Q

S

G

G

W

I

A

G

V

V

N

N

C

S

L

L

G

G

H

H

C

A

N

Y

E

P

G

K

M

L

I

T

R

E

A

A

L

L

T

K

E

E

Q

Q

A

V

G

E

K

K

L

L

I

L

N

H

P

V

S

S

P

N

A

L

Y

Y

Y

E

N

N

E

P

P

W

M

Q

A

E

R

E

L

L

A

A

G

H

L

K

L

L

T

V

K

K

N

H

S

F

C

W

F

T

D

E

-

G

K

K

V

V

F

F

T

A

N

N

S

S

G
|
G

A
x
T

E

E

A

S

N

V

E

E

G

A

A

A

I

I

K

K

L

L

A

A

R

R

K

K

-

Y

W

W

G

R

Q

D

K

K

H

G

P

K

N

N

A

-

G

-

A

-

R

K

F

W

E

K

I

F

I

I

T

S

F

F

D

E

H

N

S

S

F

F

H

H

G

G

R

R

T

T

L

Y

A

G

T

S

M

L

S

S

A

A

S

T

G

G

K

Q

P

P

G

K

W

F

D

H

K

K

I

G

F

F

A

E

P

P

Q

L

V

V

P

P

G

G

F

F

P

S

K

Y

A

A

E

K

L

L

N

N

D

D

I

I

A

D

S

S

V

V

E

Y

K

K

L

L

I

L

G

D

P

E

D

E

T

T

V

A

A

G

V

I

M

I

L

I

E

E

P

V

V

I

Q

Q

G

G

E

E

G

G

V

V

I

N

P

E

A

A

T

S

R

E

E

D

F

F

M

L

L

S

Q

K

L

L

R

Q

E

E

L

I

T

C

H

K

R

E

H

K

N

D

L

V

L

L

I

I

V

I

D

D

E

E

V

V

Q

Q

T

T

G

G

C

I

G

G

R

R

T

T

G

G

K

E

L

F

F

Y

A

A

Y

Y

E

Q

L

H

S

F

G

N

I

L

T

K

P

P

D

D

I

V

M

I

T

A

L

L

G

A

K
|
K

G

G

I

L

G

G

V

V

P

P

L

I

A

G

A

A

L

I

L

L

S

A

T

R

A

E

V

V

A

A

-

Q

V

S

F

F

E

T

A

P

G

G

D

S

Q

H

G

G

G

S

T
|
T

Y

F

N

G

G

G

N

N

P

P

L

L

M

A

T

C

A

R

V

A

G

G

I

T

S

V

V

V

I

V

E

D

Q

E

L

V

T

E

A

K

P

-

G

-

F

L

L

L

E

P

N

H

V

V

T

R

Q

E

L

V

G

G

N

N

Y

Y

L

F

R

K

E

E

Q

K

L

L

L

K

A

E

L

L

C

G

D

-

T

-

Y

-

G

-

F

-

E

-

G

-

E

-

R

K

G

G

E

K

G

V

L

K

L

G

R

R

A

G

L

L

L

M

L

L

G

G

K

L

D

E

I

L

G

E

P

R

Q

E

I

C

V

K

E

D

K

Y

A

V

R

L

N

K

L

A

S

L

P

E

D

K

G

G

L

L

V

I

N

N

A

C

A

T

R

A

P

G

N

K

L

V

L

L

R

R

F

F

M

L

P

P

A

P

L

L

N

I

V

I

T

Q

R

K

E

E

E

H

I

I

D

D

Q

R

M

A

I

I

A

S

M

V

L

L

K

R

T

E

V

I

L

L

GT 96:97 (≠ GA 102:103) binding pyridoxal 5'-phosphate
K242 (= K252) modified: N6-(pyridoxal phosphate)lysine
T271 (= T280) binding pyridoxal 5'-phosphate

2eh6A Crystal structure of acetylornithine aminotransferase from aquifex aeolicus vf5
41% identity, 96% coverage: 11:395/399 of query aligns to 3:375/375 of 2eh6A

query
sites
2eh6A

L

L

M

M

Y

N

I

N

T

Y

N

A

R

R

P

L

E

P

T

V

V

K

F

F

T

V

H

R

G

G

K

K

G

G

S

V

W

Y

I

L

F

Y

D

D

H

E

T

E

G

G

K

K

R

E

Y

Y

L

L

D

D

F

F

I

V

Q

S

G

G

W

I

A

G

V

V

N

N

C

S

L

L

G

G

H

H

C

A

N

Y

E

P

G

K

M

L

I

T

R

E

A

A

L

L

T

K

E

E

Q

Q

A

V

G

E

K

K

L

L

I

L

N

H

P

V

S

S

P

N

A

L

Y

Y

Y

E

N

N

E

P

P

W

M

Q

A

E

R

E

L

L

A

A

G

H

L

K

L

L

T

V

K

K

N

H

S

F

C

W

F

T

D

E

-

G

K

K

V

V

F

F

T

A

N

N

S

S

G
|
G

A
x
T

E

E

A

S

N

V

E

E

G

A

A

A

I

I

K

K

L

L

A

A

R

R

K

K

-

Y

W

W

G

R

Q

D

K

K

H

G

P

K

N

N

A

-

G

-

A

-

R

K

F

W

E

K

I

F

I

I

T

S

F

F

D

E

H

N

S

S

F
|
F

H
|
H

G

G

R

R

T

T

L

Y

A

G

T

S

M

L

S

S

A

A

S

T

G

G

K

Q

P

P

G

K

W

F

D

H

K

K

I

G

F

F

A

E

P

P

Q

L

V

V

P

P

G

G

F

F

P

S

K

Y

A

A

E

K

L

L

N

N

D

D

I

I

A

D

S

S

V

V

E

Y

K

K

L

L

I

L

G

D

P

E

D

E

T

T

V

A

A

G

V

I

M

I

L

I

E
|
E

P

V

V

I

Q

Q

G

G

E

E

G

G

V

V

I

N

P

E

A

A

T

S

R

E

E

D

F

F

M

L

L

S

Q

K

L

L

R

Q

E

E

L

I

T

C

H

K

R

E

H

K

N

D

L

V

L

L

I

I

V

I

D
|
D

E

E

V
|
V

Q
|
Q

T

T

G

G

C

I

G

G

R

R

T

T

G

G

K

E

L

F

F

Y

A

A

Y

Y

E

Q

L

H

S

F

G

N

I

L

T

K

P

P

D

D

I

V

M

I

T

A

L

L

G

A

K
|
K

G

G

I

L

G

G

V

V

P

P

L

I

A

G

A

A

L

I

L

L

S

A

T

R

A

E

V

V

A

A

-

Q

V

S

F

F

E

T

A

P

G

G

D

S

Q

H

G

G

G

S

T
|
T

Y

F

N

G

G

G

N

N

P

P

L

L

M

A

T

C

A

R

V

A

G

G

I

T

S

V

V

V

I

V

E

D

Q

E

L

V

T

E

A

K

P

-

G

-

F

L

L

L

E

P

N

H

V

V

T

R

Q

E

L

V

G

G

N

N

Y

Y

L

F

R

K

E

E

Q

K

L

L

L

K

A

E

L

L

C

G

D

-

T

-

Y

-

G

-

F

-

E

-

G

-

E

-

R

K

G

G

E

K

G

V

L

K

L

G

R

R

A

G

L

L

L

M

L

L

G

G

K

L

D

E

I

L

G

E

P

R

Q

E

I

C

V

K

E

D

K

Y

A

V

R

L

N

K

L

A

S

L

P

E

D

K

G

G

L

L

V

I

N

N

A

C

A

T

R

A

P

G

N

K

L

V

L

L

R
|
R

F

F

M

L

P

P

A

P

L

L

N

I

V

I

T

Q

R

K

E

E

E

H

I

I

D

D

Q

R

M

A

I

I

A

S

M

V

L

L

K

R

T

E

V

I

L

L

active site: F127 (= F138), E179 (= E190), D212 (= D223), Q215 (= Q226), K241 (= K252), T270 (= T280), R352 (= R372)
binding pyridoxal-5'-phosphate: G95 (= G102), T96 (≠ A103), F127 (= F138), H128 (= H139), E179 (= E190), D212 (= D223), V214 (= V225), K241 (= K252)

P73133 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa) (see paper)
40% identity, 97% coverage: 11:396/399 of query aligns to 35:426/429 of P73133

query
sites
P73133

L

V

M

M

Y

N

I

T

T
x
Y

N

G

R

R

P

F

E

P

T

I

V

A

F

I

T

A

H

R

G

G

K

Q

G

G

S

S

W

T

I

L

F

W

D

D

H

T

T

E

G

G

K

K

R

S

Y

Y

L

L

D

D

F

F

I

V

Q

A

G

G

W

I

A

A

V

T

N

C

C

T

L

L

G

G

H

H

C

A

N

H

E

P

G

A

M

L

I

V

R

R

A

A

L

V

T

S

E

D

Q

Q

A

I

G

Q

K

K

L

L

I

H

N

H

P

V

S

S

P

N

A

L

Y

Y

N

I

E

P

P

E

M

Q

A

G

R

E

L

L

A

A

G

K

L

W

L

I

T

V

K

E

N

H

S

S

C

C

F

A

D

D

K

R

V

V

F

F

T

C

N

N

S
|
S

G
|
G

A
|
A

E

E

A

A

N

N

E

E

G

A

A

A

I

I

K

K

L

L

A

V

R

R

K

K

W

Y

G

A

Q

-

K

-

H

H

P

T

N

V

A

L

A

D

G

F

G

L

A

E

R

Q

F

P

E

V

I

I

I

L

T

T

F

A

D

K

H

A

S

S

F

F

H

H

G

G

R
|
R

T

T

L

L

A

A

T

T

M

I

S

T

A

A

S

T

G

G

K

Q

P

P

G

K

W

Y

D

Q

K

Q

I

Y

F

F

A

D

P

P

Q

L

V

V

P

P

G

G

F

F

P

D

K

Y

A

V

E

P

L

Y

N

N

D

D

I

I

A

R

S

S

V

L

E

E

K

N

L

K

I

V

G

A

-

D

-

L

-

D

-

E

-

G

-

N

P

S

D

R

T

V

V

A

A

A

V

I

M

F

L

L

E

E

P

P

V

L

Q

Q

G

G

E
|
E

G

G

V

V

I

R

P

P

A

G

T

D

R

L

E

A

F

Y

M

F

L

K

Q

R

L

V

R

R

E

E

L

I

T

C

H

D

R

Q

H

N

N

D

L

I

L

L

I

V

V

F

D
|
D

E

E

V

V

Q
|
Q

T

V

G

G

C

V

G

G

R

R

T

T

G

G

K

K

L

L

F

W

A

G

Y

Y

E

E

L

H

S

L

G

G

I

V

T

E

P

P

D

D

I

I

M

F

T

T

L

S

G

A

K
|
K

G

G

I

L

G

A

G

G

V

V

P

P

L

I

A

G

A

A

L

M

S

C

T

K

A

K

A

F

V

C

A

D

V

V

F

F

E

E

A

P

G

G

D

N

Q

H

G

A

G

S

T
|
T

Y

F

N

G

G

G

N

N

P

P

L

L

M

A

T

C

A

A

V

A

G

G

I

L

S

A

V

V

I

L

E

K

Q

T

L

I

T

E

A

G

P

D

G

R

F

L

L

L

E

D

N

N

V

V

T

Q

Q

A

L

R

G

G

N

E

Y

Q

L

L

R

R

E

S

Q

G

L

L

L

A

A

E

L

I

C

K

D

N

T

Q

Y

Y

G

P

-

T

-

L

F

F

E

T

G

E

E

V

R

R

G

G

E

W

G

G

L

L

L

I

R

N

A

G

L

L

L

E

L

I

G

S

K

A

D

E

I

S

G

S

P

L

Q

T

I

S

V

V

E

E

K

I

A

V

R

K

N

A

L

A

S

M

P

E

D

Q

G

G

L

L

V

L

N

A

A

P

A

A

R

G

P

P

N

K

L

V

L

L

R
|
R

F

F

M

V

P

P

A

P

L

L

N

V

V

V

T

T

R

E

E

A

E

E

I

I

D

A

Q

Q

M

A

I

V

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E

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I

L

L

K

R

T

Q

V

A

L

I

A

A

Y39 (≠ T15) mutation to F: Retains 7.4% of N-acetylornithine aminotransferase wild-type activity.
S125 (= S101) mutation to A: Retains 14% of N-acetylornithine aminotransferase wild-type activity.
G126 (= G102) mutation to A: Loss of N-acetylornithine aminotransferase activity.
A127 (= A103) mutation to S: Retains 9.1% of N-acetylornithine aminotransferase wild-type activity.
R163 (= R141) mutation to A: 4100-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Retains 9.5% of N-acetylornithine aminotransferase wild-type activity.
E223 (= E195) mutation to A: 65-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Retains 50% of N-acetylornithine aminotransferase wild-type activity.; mutation to S: 73-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Shows 160% of N-acetylornithine aminotransferase wild-type activity.
D251 (= D223) mutation to A: Loss of N-acetylornithine aminotransferase activity.; mutation to E: Retains 20% of N-acetylornithine aminotransferase wild-type activity.
Q254 (= Q226) mutation to A: Retains 0.9% of N-acetylornithine aminotransferase wild-type activity.
K280 (= K252) mutation to A: Loss of N-acetylornithine aminotransferase activity.
T308 (= T280) mutation to A: Retains 0.3% of N-acetylornithine aminotransferase wild-type activity.
R402 (= R372) mutation to A: 2080-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Retains 18% of N-acetylornithine aminotransferase wild-type activity.

3nx3A Crystal structure of acetylornithine aminotransferase (argd) from campylobacter jejuni
41% identity, 95% coverage: 17:395/399 of query aligns to 10:385/388 of 3nx3A

query
sites
3nx3A

R

R

P

F

E

D

T

I

V

V

F

L

T

E

H

K

G

G

K

Q

G

G

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W

Y

I

L

F

F

D

D

H

D

T

K

G

A

K

K

R

K

Y

Y

L

L

D

D

F

F

I

S

Q

S

G

G

W

I

A

G

V

V

N

C

C

A

L

L

G

G

H

Y

C

N

N

H

E

A

G

K

M

F

I

N

R

A

A

K

L

I

T

K

E

A

Q

Q

A

V

G

D

K

K

L

L

I

L

N

H

P

T

S

S

P

N

A

L

Y

Y

N

N

E

E

P

N

M

I

A

A

R

A

L

A

A

A

G

K

L

N

L

L

T

A

K

K

N

A

S

S

C

A

F

L

D

E

K

R

V

V

F

F

T

T

N

N

S

S

G

G

A

T

E

E

A

S

N

I

E

E

G

G

A

A

I

M

K

K

L

T

A

A

R

R

K

K

W

Y

G

A

Q

-

K

-

H

F

P

N

N

K

A

G

A

V

G

K

G

G

A

G

R

Q

F

F

E

-

I

-

I

I

T

A

F

F

D

K

H

H

S

S

F
|
F

H

H

G

G

R

R

T

T

L

L

A

G

T

A

M

L

S

S

A

L

S

T

G

A

K

N

P

E

G

K

W

Y

D

Q

K

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P

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F

A

K

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P

Q

L

V

I

P

S

G

G

F

V

P

K

K

F

A

A

E

K

L

Y

N

N

D

D

I

I

A

S

S

S

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V

E

E

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K

L

L

I

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G

N

P

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D

K

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C

A

A

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I

M

I

L

L

E
|
E

P

S

V

V

Q

Q

G

G

E

E

G

G

V

I

I

N

P

P

A

A

T
x
N

R

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E

D

F
|
F

M

Y

L

K

Q

A

L

L

R

R

E

K

L

L

T

C

H

D

R

E

H

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D

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I

L

L

I

I

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A

D
|
D

E

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I

Q
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Q

T

C

G

G

C

M

G

G

R

R

T

S

G

G

K

K

L

F

F

F

A

A

Y

Y

E

E

L

H

S

A

G

Q

I

I

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L

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P

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D

I

I

M

M

T

T

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G

A

K
|
K

G

A

I

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G

G

G

C

G

G

V

L

P

S

L

V

A

G

A

A

L

F

L

V

S

I

T

N

A

Q

A

K

V

V

A

A

-

S

-

N

V

S

F

L

E

E

A

A

G

G

D

D

Q

H

G

G

G

S

T
|
T

Y

Y

N

G

G

G

N

N

P

P

L

L

M

V

T

C

A

A

V

G

G

V

I

N

S

A

V

V

I

F

E

E

Q

I

L

F

T

K

A

E

P

E

G

K

F

I

L

L

E

E

N

N

V

V

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N

Q

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G

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P

Y

Y

L

L

R

E

E

Q

Q

S

L

L

L

D

A

E

L

L

C
x
I

D

N

T

E

Y
x
F

G
x
D

F

F

E
x
C

G

K

E

K

R

R

-

K

G

G

E

L

G

G

L

F

L

M

R

Q

A

G

L

L

L

S

L

L

G

D

K

K

D

S

I

V

G

-

P

-

Q

K

I

V

V

A

E

K

K

V

A

I

R

Q

N

K

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C

S

Q

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E

D

N

G

A

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L

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L

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I

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E

N

N

L

D

L

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R
|
R

F

F

M

L

P

P

A

P

L

L

N

I

V

L

T
x
Q

R
x
K

E

E

E

H

I

I

D

D

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E

M

M

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A

E

M

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L

L

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V

A

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L

active site: F127 (= F138), E179 (= E190), D212 (= D223), Q215 (= Q226), K241 (= K252), T271 (= T280), R362 (= R372)
binding magnesium ion: N191 (≠ T202), F194 (= F205), I313 (≠ C322), F316 (≠ Y325), D317 (≠ G326), C319 (≠ E328), Q370 (≠ T380), K371 (≠ R381)

Q9M8M7 Acetylornithine aminotransferase, chloroplastic/mitochondrial; ACOAT; Acetylornithine transaminase; AOTA; Protein HOPW1-1-INTERACTING 1; EC 2.6.1.11 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
41% identity, 94% coverage: 17:391/399 of query aligns to 75:449/457 of Q9M8M7

query
sites
Q9M8M7

R

R

P

A

E

P

T

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V

F

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Y

Y

L

L

D

D

F

C

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A

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G

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I

A

A

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N

C

A

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L

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G

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A

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I

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Q

A

I

R

E

L

L

A

A

G

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L

L

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V

K

A

N

S

S

S

C

F

F

A

D

D

K

R

V

V

F

F

T

C

N

N

S

S

G

G

A

T

E

E

A

A

N

N

E

E

G

A

A

A

I

I

K

K

L

F

A

S

R

R

K

K

W

F

G

Q

Q

R

-

F

K

T

H

H

P

P

N

E

A

D

A

K

G

E

G

V

A

A

R

T

F

-

E

G

I

F

I

I

T

A

F

F

D

T

H

N

S

S

F

F

H

H

G

G

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R

T

T

L

L

A

G

T

A

M

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A

A

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S

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E

G

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Y

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I

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F

F

A

E

P

P

Q

I

V

M

P

P

G

G

F

V

P

T

K

F

A

L

E

E

L

Y

N

G

D

N

I

I

A

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A

V

A

E

T

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D

L

L

I

I

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R

P

S

D

G

T

K

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I

-

A

A

A

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V

M

F

L

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E

E

P

P

V

I

Q

Q

G

G

E

E

G

G

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I

Y

P

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A

A

T

T

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K

E

E

F

F

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L

L

Q

Q

S

L

L

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R

E

S

L

A

T

C

H

D

R

A

H

A

N

G

L

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I

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F

D

D

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E

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V

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Q

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C

G

G

C

L

G

G

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R

T

T

G

G

K

L

L

M

F

W

A

A

Y

Y

E

E

L

A

S

F

G

G

I

V

T

T

P

P

D

D

I

I

M

M

T

T

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A

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K

G

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I

L

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A

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G

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P

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I

A

G

A

A

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L

L

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T

A

E

A

K

V

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A

A

-

E

V

T

F

I

E

N

A

Y

G

G

D

D

Q

H

G

G

G

S

T

T

Y

F

N

A

G

G

N

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P

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A

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A

G

A

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A

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A

K

P

P

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F

F

L

L

E

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N

S

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N

L

K

G

G

N

R

Y

Y

L

F

R

R

E

D

Q

L

L

L

L

V

-

K

A

K

L

L

C

G

D

G

T

N

Y

S

G

H

F

V

E

K

G

E

E

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R

R

G

G

E

E

G

G

L

L

L

I

R

I

A

G

L

V

L

E

L

L

G

-

K

-

D

D

I

V

-

P

G

A

P

S

Q

S

I

L

V

V

E

D

K

A

A

C

R

R

N

D

L

-

S

-

P

-

D

S

G

G

L

L

V

I

-

L

N

T

A

A

A

G

R

K

P

G

N

N

L

V

R

R

F

I

M

V

P

P

A

P

L

L

N

V

V

I

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E

E

E

I

I

D

E

Q

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M

A

I

V

A

E

M

I

L

M

Sites not aligning to the query:

1:41 modified: transit peptide, Chloroplast and mitochondrion
42 modified: N-acetylvaline; in Acetylornithine aminotransferase, chloroplastic

P40732 Acetylornithine/succinyldiaminopimelate aminotransferase; ACOAT; DapATase; Succinyldiaminopimelate transferase; EC 2.6.1.11; EC 2.6.1.17 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
38% identity, 94% coverage: 25:398/399 of query aligns to 31:403/405 of P40732

query
sites
P40732

G

G

K

K

G

G

S

S

W

R

I

V

F

W

D

D

H

Q

T

Q

G

G

K

K

R

E

Y

Y

L

I

D

D

F

F

I

A

Q

G

G

G

W

I

A

A

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N

T

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A

L

L

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H

C

C

N

H

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G

A

M

L

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E

A

A

L

L

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E

S

Q

Q

A

G

G

E

K

T

L

L

I

W

N

H

P

T

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S

P

N

A

V

Y

F

Y

T

N

N

E

E

P

P

M

A

A

L

R

R

L

L

A

G

G

R

L

K

L

L

T

I

K

D

N

A

S

T

C

F

F

A

D

E

K

R

V

V

F

L

F

F

T

M

N

N

S

S

G
|
G

A
x
T

E

E

A

A

N

N

E

E

G

T

A

A

I

F

K

K

L

L

A

A

R

R

K

H

W

Y

G

A

Q

C

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V

H

R

P

H

N

S

A

P

A

F

G

-

A

-

R

K

F

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E

K

I

I

T

A

F

F

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H

H

N

S

A

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F

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H

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G

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R

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L

L

A

F

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M

V

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A

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S

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P

G

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D

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F

A

G

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P

Q

K

V

P

P

A

G

D

F

I

P

I

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H

A

V

E

P

L

F

N

N

D

D

I

L

A

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A

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E

K

K

A

L

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G

D

P

D

D

H

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C

A

A

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M

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L

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E

E

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I

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Q

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G

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A

A

A

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T

R

P

E

E

F

F

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L

L

K

Q

G

L

L

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R

E

D

L

L

T

C

H

D

R

E

H

H

N

Q

L

A

L

L

I

V

V

F

D

D

E

E

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V

Q

Q

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C

G

G

C

M

G

G

R

R

T

T

G

G

K

D

L

L

F

F

A

A

Y

Y

E

M

L

H

S

Y

G

G

I

V

T

T

P

P

D

D

I

I

M

L

T

T

L

S

G

A

K
|
K

G

A

I

L

G

G

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F

P

P

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V

A

S

A

A

L

M

L

L

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T

T

T

A

Q

A

E

V

I

A

A

-

S

V

A

F

F

E

H

A

V

G

G

D

S

Q

H

G

G

G

S

T
|
T

Y

Y

N

G

G

G

N

N

P

P

L

L

M

A

T

C

A

A

V

V

G

A

I

G

S

A

V

A

I

F

E

D

Q

I

L

I

T

N

A

T

P

P

G

E

F

V

L

L

E

Q

N

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I

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Q

T

L

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G

R

N

Q

Y

Q

L

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R

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E

Q

Q

H

L

L

L

Q

A

A

L

I

C

D

D

E

T

Q

Y

F

G

D

-

I

F

F

E

S

G

D

E

I

R

R

G

G

E

M

G

G

L

-

R

-

A

-

L

L

L

I

G

G

K

A

D

E

I

L

G

K

P

P

Q

K

I

Y

V

K

E

G

K

R

A

A

R

R

N

D

L

F

-

L

-

Y

-

A

-

G

S

A

P

E

D

A

G

G

L

V

L

M

V

V

N

L

A

N

A

A

R

G

P

A

N

D

L

V

L

M

R

R

F

F

M

A

P

P

A

S

L

L

N

V

V

V

T

E

R

E

E

A

E

D

I

I

D

H

Q

E

M

G

I

M

A

Q

M

R

L

F

K

A

T

Q

V

A

L

V

A

G

Q

K

V

V

GT 108:109 (≠ GA 102:103) binding pyridoxal 5'-phosphate
K255 (= K252) modified: N6-(pyridoxal phosphate)lysine
T284 (= T280) binding pyridoxal 5'-phosphate

4jevB N-acetylornithine aminotransferase from s. Typhimurium complexed with gabaculine
38% identity, 94% coverage: 25:398/399 of query aligns to 26:398/402 of 4jevB

query
sites
4jevB

G

G

K

K

G

G

S

S

W

R

I

V

F

W

D

D

H

Q

T

Q

G

G

K

K

R

E

Y

Y

L

I

D

D

F

F

I

A

Q

G

G

G

W
x
I

A

A

V

V

N

T

C

A

L

L

G

G

H

H

C

C

N

H

E

P

G

A

M

L

I

V

R

E

A

A

L

L

T

K

E

S

Q

Q

A

G

G

E

K

T

L

L

I

W

N

H

P

T

S

S

P

N

A

V

Y

F

Y

T

N

N

E

E

P

P

M

A

A

L

R

R

L

L

A

G

G

R

L

K

L

L

T

I

K

D

N

A

S

T

C

F

F

A

D

E

K

R

V

V

F

L

F

F

T

M

N

N

S
|
S

G
|
G

A
x
T

E

E

A

A

N

N

E

E

G

T

A

A

I

F

K

K

L

L

A

A

R

R

K

H

W

Y

G

A

Q

C

K

V

H

R

P

H

N

S

A

P

A

F

G

-

A

-

R

K

F

T

E

K

I

I

T

A

F

F

D

H

H

N

S

A

F
|
F

H
|
H

G

G

R

R

T

S

L

L

A

F

T

T

M

V

S

S

A

V

S

G

G

G

K

Q

P

P

G

K

W

Y

D

S

K

D

I

G

F

F

A

G

P

P

Q

K

V

P

P

A

G

D

F

I

P

I

K

H

A

V

E

P

L

F

N

N

D

D

I

L

A

H

S

A

V

V

E

K

K

A

L

V

I

M

G

D

P

D

D

H

T

T

V

C

A

A

V

V

M

V

L

V

E
|
E

P

P

V

I

Q

Q

G

G

E
|
E

G

G

V

V

I

Q

P

A

A

A

T

T

R

P

E

E

F

F

M

L

L

K

Q

G

L

L

R

R

E

D

L

L

T

C

H

D

R

E

H

H

N

Q

L

A

L

L

I

V

V

F

D
|
D

E

E

V
|
V

Q
|
Q

T

C

G

G

C

M

G

G

R

R

T

T

G

G

K

D

L

L

F

F

A

A

Y

Y

E

M

L

H

S

Y

G

G

I

V

T

T

P

P

D

D

I

I

M

L

T

T

L

S

G

A

K
|
K

G

A

I

L

G

G

V

F

P

P

L

V

A

S

A

A

L

M

L

L

S

T

T

T

A

Q

A

E

V

I

A

A

-

S

V

A

F

F

E

H

A

V

G

G

D

S

Q

H

G

G

G

S

T
|
T

Y

Y

N

G

G

G

N

N

P

P

L

L

M

A

T

C

A

A

V

V

G

A

I

G

S

A

V

T

I

F

E

D

Q

I

L

I

T

N

A

T

P

P

G

E

F

V

L

L

E

Q

N

G

V

I

T

H

Q

T

L

K

G

R

N

Q

Y

Q

L

F

R

V

E

Q

Q

H

L

L

L

Q

A

A

L

I

C

D

D

E

T

Q

Y

F

G

D

-

I

F

F

E

S

G

D

E

I

R

R

G

G

E

M

G

G

L

-

R

-

A

-

L

L

L

I

G

G

K

A

D

E

I

L

G

K

P

P

Q

K

I

Y

V

K

E

G

K

R

A

A

R

R

N

D

L

F

-

L

-

Y

-

A

-

G

S

A

P

E

D

A

G

G

L

V

L

M

V

V

N

L

A

N

A

A

R

G

P

A

N

D

L

V

L

M

R
|
R

F

F

M

A

P

P

A

S

L

L

N

V

V

V

T

E

R

E

E

A

E

D

I

I

D

H

Q

E

M

G

I

M

A

Q

M

R

L

F

K

A

T

Q

V

A

L

V

A

G

Q

K

V

V

active site: F136 (= F138), E188 (= E190), D221 (= D223), Q224 (= Q226), K250 (= K252), T279 (= T280), R372 (= R372)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: I46 (≠ W45), S102 (= S101), G103 (= G102), T104 (≠ A103), F136 (= F138), H137 (= H139), E188 (= E190), E193 (= E195), D221 (= D223), V223 (= V225), Q224 (= Q226), K250 (= K252), R372 (= R372)

4jewA N-acetylornithine aminotransferase from s. Typhimurium complexed with l-canaline
37% identity, 94% coverage: 25:398/399 of query aligns to 26:393/397 of 4jewA

query
sites
4jewA

G

G

K
|
K

G

G

S

S

W

R

I

V

F
x
W

D

D

H

Q

T

Q

G

G

K

K

R

E

Y

Y

L

I

D

D

F

F

I

A

Q

G

G

G

W

I

A

A

V

V

N

T

C

A

L

L

G

G

H

H

C

C

N

H

E

P

G

A

M

L

I

V

R

E

A

A

L

L

T

K

E

S

Q

Q

A

G

G

E

K

T

L

L

I

W

N

H

P

T

S

S

P

N

A

V

Y

F

Y

T

N

N

E

E

P

P

M

A

A

L

R

R

L

L

A

G

G

R

L

K

L

L

T

I

K

D

N

A

S

T

C

F

F

A

D

E

K

R

V

V

F

L

F

F

T

M

N

N

S

S

G
|
G

A
x
T

E

E

A

A

N

N

E

E

G

T

A

A

I

F

K

K

L

L

A

A

R

R

K

H

W

Y

G

A

Q

C

K

V

H

R

P

H

N

S

A

P

A

F

G

-

A

-

R

K

F

T

E

K

I

I

T

A

F

F

D

H

H

N

S

A

F
|
F

H
|
H

G

G

R
|
R

T

S

L

L

A

F

T

T

M

V

S

S

A

V

S

G

G

G

K

Q

P

P

G

K

W

Y

D

S

K

D

I

G

F

F

A

G

P

P

Q

K

V

P

P

A

G

D

F

I

P

I

K

H

A

V

E

P

L

F

N

N

D

D

I

L

A

H

S

A

V

V

E

K

K

A

L

V

I

M

G

D

P

D

D

H

T

T

V

C

A

A

V

V

M

V

L

V

E
|
E

P

P

V

I

Q

Q

G

G

E
|
E

G

G

V

V

I

Q

P

A

A

A

T

T

R

P

E

E

F

F

M

L

L

K

Q

G

L

L

R

R

E

D

L

L

T

C

H

D

R

E

H

H

N

Q

L

A

L

L

I

V

V

F

D
|
D

E

E

V
|
V

Q
|
Q

T

C

G

G

C

M

G

G

R

R

T

T

G

G

K

D

L

L

F

F

A

A

Y

Y

E

M

L

H

S

Y

G

G

I

V

T

T

P

P

D

D

I

I

M

L

T

T

L

S

G

A

K
|
K

G

A

I

L

G

G

V

F

P

P

L

V

A

S

A

A

L

M

L

L

S

T

T

T

A

Q

A

E

V

I

A

-

V

-

F

-

E

-

A

A

G

S

D

A

Q

F

G

G

G

S

T
|
T

Y

Y

N

G

G

G

N

N

P

P

L

L

M

A

T

C

A

A

V

V

G

A

I

G

S

A

V

T

I

F

E

D

Q

I

L

I

T

N

A

T

P

P

G

E

F

V

L

L

E

Q

N

G

V

I

T

H

Q

T

L

K

G

R

N

Q

Y

Q

L

F

R

V

E

Q

Q

H

L

L

L

Q

A

A

L

I

C

D

D

E

T

Q

Y

F

G

D

-

I

F

F

E

S

G

D

E

I

R

R

G

G

E

M

G

G

L

-

R

-

A

-

L

L

L

I

G

G

K

A

D

E

I

L

G

K

P

P

Q

K

I

Y

V

K

E

G

K

R

A

A

R

R

N

D

L

F

-

L

-

Y

-

A

-

G

S

A

P

E

D

A

G

G

L

V

L

M

V

V

N

L

A

N

A

A

R
</