SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_048051364.1 NCBI__GCF_000744315.1:WP_048051364.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
37% identity, 94% coverage: 17:255/255 of query aligns to 26:261/265 of P07821

query
sites
P07821

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K50 (= K41) mutation to Q: Lack of activity.
D172 (= D161) mutation to E: Lack of activity.
E173 (= E162) mutation to A: Lack of activity.

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
36% identity, 85% coverage: 12:227/255 of query aligns to 12:226/241 of 4u00A

query
sites
4u00A

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binding adenosine-5'-diphosphate: F12 (≠ Y12), V17 (= V17), S37 (≠ N37), G38 (= G38), S39 (≠ A39), G40 (= G40), K41 (= K41), S42 (= S42), T43 (= T43)

3c4jA Abc protein artp in complex with atp-gamma-s
32% identity, 86% coverage: 3:221/255 of query aligns to 4:222/242 of 3c4jA

query
sites
3c4jA

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binding phosphothiophosphoric acid-adenylate ester: F13 (≠ Y12), V18 (= V17), S38 (≠ N37), G39 (= G38), S40 (≠ A39), G41 (= G40), K42 (= K41), S43 (= S42), T44 (= T43)

3c41J Abc protein artp in complex with amp-pnp/mg2+
32% identity, 86% coverage: 3:221/255 of query aligns to 4:222/242 of 3c41J

query
sites
3c41J

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binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ Y12), V18 (= V17), S38 (≠ N37), G39 (= G38), G41 (= G40), K42 (= K41), S43 (= S42), T44 (= T43)
binding magnesium ion: S43 (= S42), D163 (= D161)

2olkA Abc protein artp in complex with adp-beta-s
32% identity, 86% coverage: 3:221/255 of query aligns to 4:222/242 of 2olkA

query
sites
2olkA

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binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (≠ Y12), V18 (= V17), S38 (≠ N37), G39 (= G38), S40 (≠ A39), G41 (= G40), K42 (= K41), S43 (= S42), T44 (= T43)

2oljA Abc protein artp in complex with adp/mg2+
32% identity, 86% coverage: 3:221/255 of query aligns to 4:222/242 of 2oljA

query
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2oljA

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F

Q

Q

E

R

L

F

S

N

T

L

T

F

F

P

Q

H

A

M

T

T

V

V

F

L

D

N

T

N

V

I

L

T

M

L

G

A

R

P

R

M

P

K

F

V

S

R

S

K

W

W

R

P

I

R

S

E

K

K

D

A

D

E

V

-

E

-

I

-

A

-

A

A

R

K

A

A

I

M

K

E

M

L

L

L

N

D

-

K

-

V

-

G

L

L

E

K

Q

D

V

K

S

A

L

H

R

A

Y

Y

N

P

S

D

E

S

L

L

S

S

G

G

Q

Q

Q

A

Q

Q

R

R

V

V

M

A

I

I

A

A

R

R

A

A

L

L

A

A

Q

M

N

E

S

P

R

K

V

I

L

M

L

L

L

F

D

D

E

E

P

P

T

T

S

S

K

A

L

L

D

D

I

P

R

E

N

M

Q

V

L

G

E

E

V

V

M

L

D

S

I

V

I

M

R

K

A

Q

L

L

V

A

E

-

K

N

Q

E

E

G

L

M

L

T

S

M

I

V

L

V

V

V

I

T

H

H

D

E

L

M

N

G

L

F

A

A

S

R

R

E

Y

V

T

G

D

D

K

R

I

V

I

L

M

F

L

M

K

D

G

G

K

Y

I

I

F

I

A

E

G

G

D

K

P

P

binding adenosine-5'-diphosphate: F13 (≠ Y12), V18 (= V17), S38 (≠ N37), G39 (= G38), S40 (≠ A39), G41 (= G40), K42 (= K41), S43 (= S42), T44 (= T43)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
33% identity, 87% coverage: 5:225/255 of query aligns to 4:224/240 of 4ymuJ

query
sites
4ymuJ

I

V

D

N

G

D

V

V

K

Y

L

K

S

N

Y
x
F

A

G

S

S

V

L

P

E

V
|
V

L

L

H

K

G

G

I

V

S

T

M

L

E

K

F

V

C

N

K

K

P

G

C

E

V

V

G

V

I

I

G

G

P

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

C

C

I

I

N

N

N

L

V

L

L

E

K

E

P

P

K

T

S

K

G

G

W

E

V

V

C

F

L

I

D

D

G

G

Q

V

K

K

T

I

R

N

D

N

-

G

-

K

M

V

D

N

A

I

V

N

E

K

I

V

A

R

R

Q

N

K

F

V

G

G

Y

M

V

V

P

F

Q

Q

E

H

L

F

S

N

T

L

T

F

F

P

Q

H

A

L

T

T

V

A

F

I

D

E

T

N

V

I

L

T

M

L

G

A

R

-

P

P

F

V

S

K

S

V

W

K

R

K

I

M

S

N

K

K

D

K

D

E

V

A

-

E

E

E

I

L

A

A

A

V

R

D

A

L

I

L

K

A

M

K

L

V

N

G

L

L

E

L

Q

D

V

K

S

K

L

D

R

Q

Y

Y

N

P

S

I

E

K

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

V

L

M

A

I

I

A

A

R

R

A

A

L

L

A

A

Q

M

N

Q

S

P

R

E

V

V

L

M

L

L

L

F

D

D

E
|
E

P

P

T

T

S

S

K

A

L

L

D

D

I

P

R

E

N

M

Q

V

L

K

E

E

V

V

M

L

D

N

I

V

I

M

R

K

A

Q

L

L

V

A

E

-

K

N

Q

E

E

G

L

M

L

T

S

M

I

V

L

V

V

V

I

T

H
|
H

D

E

L

M

N

G

L

F

A

A

S

R

R

E

Y

V

T

G

D

D

K

R

I

V

I

I

M

F

L

M

K

D

G

D

G

G

K

V

I

I

F

V

A

E

G

G

D

T

P

P

V

E

S

E

V

I

L

F

binding adenosine-5'-triphosphate: F11 (≠ Y12), V16 (= V17), S36 (≠ N37), G37 (= G38), S38 (≠ A39), G39 (= G40), K40 (= K41), S41 (= S42), T42 (= T43), E162 (= E162), H194 (= H195)
binding magnesium ion: S41 (= S42), E162 (= E162)

1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
33% identity, 81% coverage: 9:215/255 of query aligns to 12:223/232 of 1f3oA

query
sites
1f3oA

K

K

L

M

S

G

Y

E

A

E

S

I

V

I

P

Y

V

A

L

L

H

K

G

N

I

V

S

N

M

L

E

N

F

I

C

K

K

E

P

G

C

E

V

F

V

V

G

S

I

I

I

M

G

G

P

P

N

S

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

L

M

L

L

R

N

C

I

I

I

N

G

N

C

V

L

L

D

K

K

P

P

K

T

S

E

G

G

W

E

V

V

C

Y

L

I

D

D

G

N

Q

I

K

K

T

T

R

N

D

D

M

L

D

D

A

D

V

D

E

E

I

L

A

T

R

K

-

I

-

R

-

D

N

K

F

I

G

G

Y

F

V

V

P

F

Q

Q

E

Q

L

F

S

N

T

L

T

I

F

P

Q

L

A

L

T

T

V

A

F

L

D

E

T

N

V

V

L

E

M

L

G

-

R

-

P

P

F

L

-

I

S

F

S

K

W

Y

R

R

I

G

S

A

K

M

D

S

D

G

V

E

E

E

I

R

A

R

A

K

R

R

A

A

I

L

K

E

M

C

L

L

N

K

L

M

E

A

Q

E

V

L

S

E

L

E

R

R

Y

F

N

A

S

N

-

H

-

K

-

P

-

N

E

Q

L

L

S

S

G

G

Q

Q

R

R

V

V

M

A

I

I

A

A

R

R

A

A

L

L

A

A

Q

N

N

N

S

P

R

P

V

I

L

I

L

L

L

A

D

D

E

E

P

P

T

T

S

G

K

A

L

L

D

D

I

S

R

K

N

T

Q

G

L

E

E

K

V

I

M

M

D

Q

I

L

R

K

A

K

L

L

V

N

E

E

K

E

Q

D

E

G

L

K

L

T

S

V

I

V

L

V

V

V

I

T

H

H

D

D

L

I

N

N

L

V

A

A

S

-

R

R

Y

F

T

G

D

E

K

R

I

I

M

Y

L

L

K

K

G

D

G

G

K

E

I

V

binding adenosine-5'-diphosphate: G41 (= G38), G43 (= G40), K44 (= K41), S45 (= S42), T46 (= T43)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 11

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
31% identity, 87% coverage: 14:234/255 of query aligns to 17:229/343 of P30750

query
sites
P30750

S

T

V

I

P

Q

V

A

L

L

H

N

G

N

I

V

S

S

M

L

E

H

F

V

C

P

K

A

P

G

C

Q

V

I

V

Y

G

G

I

V

I

I

G

G

P

A

N
x
S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

R

R

C

C

I

V

N

N

N

L

V

L

L

E

K

R

P

P

K

T

S

E

G

G

W

S

V

V

C

L

L

V

D

D

G

G

Q

Q

K

E

T

L

R

T

D

T

M

L

D

S

A

E

V

S

E

E

I

L

A

T

-

K

-

A

-

R

R

R

N

Q

F

I

G

G

Y

M

V

I

P

F

Q

Q

E

H

L

F

S

N

T

L

F

S

Q

S

A

R

T

T

V

V

F

F

D

G

T

N

V

V

L

A

M

L

G

-

R

-

P

P

F

L

S

E

S

L

W

D

R

N

I

T

S

P

K

K

D

D

D

-

V

-

E

E

I

V

A

K

A

R

R

R

A

V

I

T

K

E

M

L

L

L

N

S

L

L

E

V

Q

G

V

L

S

G

L

D

R

K

-

H

-

D

-

S

Y

Y

N

P

S

S

E

N

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

V

V

M

A

I

I

A

A

R

R

A

A

L

L

A

A

Q

S

N

N

S

P

R

K

V

V

L

L

L

C

D

D

E
|
E

P

A

T

T

S

S

K

A

L

L

D

D

I

P

R

A

N

T

Q

T

L

R

E

S

V

I

M

L

D

E

I

L

R

K

A

D

L

I

V

N

E

R

K

R

Q

L

E

G

L

L

L

T

S

I

I

L

L

L

V

I

I

T

H

H

D

E

L

M

N

D

L

V

A

V

S

K

R

R

Y

I

T

C

D

D

K

C

I

V

I

A

M

V

L

I

K

S

G

N

G

G

K

E

I

L

F

-

A

-

G

-

D

-

P

-

V

-

S

-

V

-

L

I

T

E

Q

Q

D

D

N

T

I

V

R

S

A

E

V

V

Y

F

40:46 (vs. 37:43, 86% identical) binding ATP
E166 (= E162) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding L-methionine
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding L-methionine

1l2tA Dimeric structure of mj0796, a bacterial abc transporter cassette (see paper)
33% identity, 81% coverage: 9:215/255 of query aligns to 12:223/230 of 1l2tA

query
sites
1l2tA

K

K

L

M

S

G

Y

E

A

E

S

I

V

I

P

Y

V

A

L

L

H

K

G

N

I

V

S

N

M

L

E

N

F

I

C

K

K

E

P

G

C

E

V

F

V

V

G

S

I

I

I

M

G

G

P

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

M

L

L

R

N

C

I

I

I

N

G

N

C

V

L

L

D

K

K

P

P

K

T

S

E

G

G

W

E

V

V

C

Y

L

I

D

D

G

N

Q

I

K

K

T

T

R

N

D

D

M

L

D

D

A

D

V

D

E

E

I

L

A

T

R

K

-

I

-

R

-

D

N

K

F

I

G

G

Y

F

V

V

P

F

Q

Q

E

Q

L

F

S

N

T

L

T

I

F

P

Q

L

A

L

T

T

V

A

F

L

D

E

T

N

V

V

L

E

M

L

G

-

R

-

P

P

F

L

-

I

S

F

S

K

W

Y

R

R

I

G

S

A

K

M

D

S

D

G

V

E

E

E

I

R

A

R

A

K

R

R

A

A

I

L

K

E

M

C

L

L

N

K

L

M

E

A

Q

E

V

L

S

E

L

E

R

R

Y
x
F

N

A

S

N

-

H

-

K

-

P

-

N

E
x
Q

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

Q

R

R

V

V

M

A

I

I

A

A

R

R

A

A

L

L

A

A

Q

N

N

N

S

P

R

P

V

I

L

I

L

L

L

A

D

D

E

Q

P

P

T

T

S

G

K

A

L

L

D

D

I

S

R

K

N

T

Q

G

L

E

E

K

V

I

M

M

D

Q

I

L

R

K

A

K

L

L

V

N

E

E

K

E

Q

D

E

G

L

K

L

T

S

V

I

V

L

V

V

V

I

T

H
|
H

D

D

L

I

N

N

L

V

A

A

S

-

R

R

Y

F

T

G

D

E

K

R

I

I

M

Y

L

L

K

K

G

D

G

G

K

E

I

V

binding adenosine-5'-triphosphate: S40 (≠ N37), G41 (= G38), S42 (≠ A39), G43 (= G40), K44 (= K41), S45 (= S42), T46 (= T43), F138 (≠ Y133), Q145 (≠ E136), S147 (= S138), G149 (= G140), Q150 (= Q141), H204 (= H195)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 11

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
31% identity, 87% coverage: 14:234/255 of query aligns to 18:230/344 of 6cvlD

query
sites
6cvlD

S

T

V
x
I

P

Q

V

A

L

L

H

N

G

N

I

V

S

S

M

L

E

H

F

V

C

P

K

A

P

G

C

Q

V

I

V

Y

G

G

I

V

I

I

G

G

P

A

N
x
S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

R

R

C

C

I

V

N

N

N

L

V

L

L

E

K

R

P

P

K

T

S

E

G

G

W

S

V

V

C

L

L

V

D

D

G

G

Q

Q

K

E

T

L

R

T

D

T

M

L

D

S

A

E

V

S

E

E

I

L

A

T

-

K

-

A

-

R

R

R

N

Q

F

I

G

G

Y

M

V

I

P

F

Q

Q

E

H

L

F

S

N

T

L

F

S

Q

S

A

R

T

T

V

V

F

F

D

G

T

N

V

V

L

A

M

L

G

-

R

-

P

P

F

L

S

E

S

L

W

D

R

N

I

T

S

P

K

K

D

D

D

-

V

-

E

E

I

V

A

K

A

R

R

R

A

V

I

T

K

E

M

L

L

L

N

S

L

L

E

V

Q

G

V

L

S

G

L

D

R

K

-

H

-

D

-

S

Y

Y

N

P

S

S

E
x
N

L

L

S
|
S

G

G

Q
|
Q

Q

K

Q

Q

R

R

V

V

M

A

I

I

A

A

R

R

A

A

L

L

A

A

Q

S

N

N

S

P

R

K

V

V

L

L

L

C

D

D

E

Q

P

A

T

T

S

S

K

A

L

L

D

D

I

P

R

A

N

T

Q

T

L

R

E

S

V

I

M

L

D

E

I

L

R

K

A

D

L

I

V

N

E

R

K

R

Q

L

E

G

L

L

L

T

S

I

I

L

L

L

V

I

I

T

H
|
H

D

E

L

M

N

D

L

V

A

V

S

K

R

R

Y

I

T

C

D

D

K

C

I

V

I

A

M

V

L

I

K

S

G

N

G

G

K

E

I

L

F

-

A

-

G

-

D

-

P

-

V

-

S

-

V

-

L

I

T

E

Q

Q

D

D

N

T

I

V

R

S

A

E

V

V

Y

F

binding phosphothiophosphoric acid-adenylate ester: I19 (≠ V15), S41 (≠ N37), G42 (= G38), A43 (= A39), G44 (= G40), K45 (= K41), S46 (= S42), T47 (= T43), N141 (≠ E136), S143 (= S138), Q146 (= Q141), H200 (= H195)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 12, 14

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
31% identity, 87% coverage: 14:234/255 of query aligns to 18:230/344 of 3tuzC

query
sites
3tuzC

S

T

V

I

P

Q

V

A

L

L

H

N

G

N

I

V

S

S

M

L

E

H

F

V

C

P

K

A

P

G

C

Q

V

I

V

Y

G

G

I

V

I

I

G

G

P

A

N

S

G
|
G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

R

R

C

C

I

V

N

N

N

L

V

L

L

E

K

R

P

P

K

T

S

E

G

G

W

S

V

V

C

L

L

V

D

D

G

G

Q

Q

K

E

T

L

R

T

D

T

M

L

D

S

A

E

V

S

E

E

I

L

A

T

-

K

-

A

-

R

R

R

N

Q

F

I

G

G

Y

M

V

I

P

F

Q

Q

E

H

L

F

S

N

T

L

F

S

Q

S

A

R

T

T

V

V

F

F

D

G

T

N

V

V

L

A

M

L

G

-

R

-

P

P

F

L

S

E

S

L

W

D

R

N

I

T

S

P

K

K

D

D

D

-

V

-

E

E

I

V

A

K

A

R

R

R

A

V

I

T

K

E

M

L

L

L

N

S

L

L

E

V

Q

G

V

L

S

G

L

D

R

K

-

H

-

D

-

S

Y

Y

N

P

S

S

E

N

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

V

V

M

A

I

I

A

A

R

R

A

A

L

L

A

A

Q

S

N

N

S

P

R

K

V

V

L

L

L

C

D

D

E

Q

P

A

T

T

S

S

K

A

L

L

D

D

I

P

R

A

N

T

Q

T

L

R

E

S

V

I

M

L

D

E

I

L

R

K

A

D

L

I

V

N

E

R

K

R

Q

L

E

G

L

L

L

T

S

I

I

L

L

L

V

I

I

T

H

H

D

E

L

M

N

D

L

V

A

V

S

K

R

R

Y

I

T

C

D

D

K

C

I

V

I

A

M

V

L

I

K

S

G

N

G

G

K

E

I

L

F

-

A

-

G

-

D

-

P

-

V

-

S

-

V

-

L

I

T

E

Q

Q

D

D

N

T

I

V

R

S

A

E

V

V

Y

F

binding adenosine-5'-diphosphate: G42 (= G38), G44 (= G40), K45 (= K41), S46 (= S42), T47 (= T43)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 14
binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
31% identity, 87% coverage: 14:234/255 of query aligns to 18:230/344 of 3tuiC

query
sites
3tuiC

S

T

V

I

P

Q

V

A

L

L

H

N

G

N

I

V

S

S

M

L

E

H

F

V

C

P

K

A

P

G

C

Q

V

I

V

Y

G

G

I

V

I

I

G

G

P

A

N

S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

R

R

C

C

I

V

N

N

N

L

V

L

L

E

K

R

P

P

K

T

S

E

G

G

W

S

V

V

C

L

L

V

D

D

G

G

Q

Q

K

E

T

L

R

T

D

T

M

L

D

S

A

E

V

S

E

E

I

L

A

T

-

K

-

A

-

R

R

R

N

Q

F

I

G

G

Y

M

V

I

P

F

Q

Q

E

H

L

F

S

N

T

L

F

S

Q

S

A

R

T

T

V

V

F

F

D

G

T

N

V

V

L

A

M

L

G

-

R

-

P

P

F

L

S

E

S

L

W

D

R

N

I

T

S

P

K

K

D

D

D

-

V

-

E

E

I

V

A

K

A

R

R

R

A

V

I

T

K

E

M

L

L

L

N

S

L

L

E

V

Q

G

V

L

S

G

L

D

R

K

-

H

-

D

-

S

Y

Y

N

P

S

S

E

N

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

V

V

M

A

I

I

A

A

R

R

A

A

L

L

A

A

Q

S

N

N

S

P

R

K

V

V

L

L

L

C

D

D

E

Q

P

A

T

T

S

S

K

A

L

L

D

D

I

P

R

A

N

T

Q

T

L

R

E

S

V

I

M

L

D

E

I

L

R

K

A

D

L

I

V

N

E

R

K

R

Q

L

E

G

L

L

L

T

S

I

I

L

L

L

V

I

I

T

H

H

D

E

L

M

N

D

L

V

A

V

S

K

R

R

Y

I

T

C

D

D

K

C

I

V

I

A

M

V

L

I

K

S

G

N

G

G

K

E

I

L

F

-

A

-

G

-

D

-

P

-

V

-

S

-

V

-

L

I

T

E

Q

Q

D

D

N

T

I

V

R

S

A

E

V

V

Y

F

binding adenosine-5'-diphosphate: G42 (= G38), A43 (= A39), G44 (= G40), K45 (= K41), S46 (= S42), T47 (= T43)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

5x40A Structure of a cbio dimer bound with amppcp (see paper)
35% identity, 85% coverage: 10:226/255 of query aligns to 10:229/280 of 5x40A

query
sites
5x40A

L

L

S

T

Y

Y

A

A

-
x
F

-

P

-

G

S

G

V
|
V

P

K

V
x
A

L

L

H

D

G

D

I

L

S

S

M

L

E

A

F

V

C

P

K

K

P

G

C

E

V

S

V

L

G

A

I

I

I

L

G

G

P

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

L

C

H

I

L

N

N

N

G

V

T

L

L

K

R

P

P

K

Q

S

S

G

G

W

R

V

V

C

L

L

L

D

G

G

G

Q

T

K

A

T

T

R

G

D

H

-

S

-

R

M

K

D

D

A

L

V

T

E

G

I

W

A

R

R

R

N

R

F

V

G

G

Y

L

V

V

P

L

Q
|
Q

E

D

L

A

S

D

T

D

T

Q

-

L

F

F

Q

A

A

T

T

T

V

V

F

F

D

E

T

D

V

V

L

-

M

-

G

-

R

-

P

S

F

F

S

G

S

P

W

L

R

N

I

L

S

G

K

L

D

S

D

E

V

A

E

E

I

A

A

R

A

A

R

R

-

V

-

E

A

A

I

L

K

A

M

A

L

L

N

S

L

I

E

S

Q

D

V

L

S

R

L

D

R

R

Y

P

N

T

S
x
H

E
x
M

L
|
L

S
|
S

G

G

G
|
G

Q
|
Q

Q

K

Q

R

R

R

V

V

M

A

I

I

A

A

R

G

A

A

L

V

A

A

Q

M

N

R

S

P

R

E

V

V

L

L

D

D

E
x
Q

P

P

T

T

S

A

K

G

L

L

D

D

I

L

R

A

N

G

Q

T

L

E

E

Q

V

L

M

L

D

T

I

L

R

R

A

G

L

L

V

R

E

A

K

A

Q

G

E

M

L

T

L

L

S

-

I

V

L

F

V

S

I

T

H
|
H

D

D

L

V

N

E

L

L

A

A

S

A

R

A

Y

L

T

A

D

D

K

R

I

V

I

A

M

L

L

F

K

R

G

T

G

G

K

R

I

V

F

L

A

A

A

E

G

G

D

A

P

A

V

E

S

A

V

V

L

L

T

S

binding phosphomethylphosphonic acid adenylate ester: F14 (vs. gap), V18 (= V15), A20 (≠ V17), N40 (= N37), G41 (= G38), G43 (= G40), K44 (= K41), S45 (= S42), T46 (= T43), Q88 (= Q83), H139 (≠ S135), M140 (≠ E136), L141 (= L137), S142 (= S138), G144 (= G140), Q145 (= Q141), Q166 (≠ E162), H198 (= H195)
binding magnesium ion: S45 (= S42), Q88 (= Q83)

7arlD Lolcde in complex with lipoprotein and adp (see paper)
36% identity, 77% coverage: 17:212/255 of query aligns to 21:218/222 of 7arlD

query
sites
7arlD

V

V

L

L

H

H

G

N

I

V

S

S

M

F

E

S

F

V

C

G

K

E

P

G

C

E

V

M

G

A

I

I

I

V

G

G

P

S

N

S

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T

T

L

L

R

H

C

L

I

L

N

G

V

L

L

D

K

T

P

P

K

T

S

S

G

G

W

D

V

V

C

I

L

F

D

N

G

G

Q

Q

K

P

T

M

R

S

D

K

M

L

D

S

A

S

V

A

E

A

I

K

A

A

-

E

-

L

R

R

N

N

-

Q

-

K

F

L

G

G

Y

F

V

I

P

Y

Q

Q

E

F

L

H

S

H

T

L

F

P

Q

D

A

F

T

T

V

A

F

L

D

E

T

N

V

V

L

A

M

M

G

-

R

-

P

P

F

L

S

L

S

I

W

G

R

K

I

-

S

-

K

K

D

K

D

P

V

A

E

E

I

I

A

N

A

S

R

R

A

A

I

L

K

E

M

M

L

L

N

K

L

A

E

V

Q

G

V

L

S

D

L

H

R

R

Y

A

N

N

-

H

-

R

-

P

S

S

E

E

L

L

S

S

G

G

Q

E

Q

R

Q

Q

R

R

V

V

M

A

I

I

A

A

R

R

A

A

L

L

A

V

Q

N

N

N

S

P

R

R

V

L

L

V

L

L

L

A

D

D

E

E

P

P

T

T

S

G

K

N

L

L

D

D

I

A

R

R

N

N

Q

A

L

D

E

S

V

I

M

F

D

Q

I

L

R

G

A

E

L

L

V

N

E

R

K

L

Q

Q

E

G

L

T

L

A

S

F

I

L

L

V

V

V

I

T

H

H

D

D

L

L

N

Q

L

L

A

A

S

K

R

R

Y

M

T

S

D

R

K

Q

I

L

I

E

M

M

L

R

K

D

G

G

binding adenosine-5'-diphosphate: G42 (= G38), G44 (= G40), K45 (= K41), S46 (= S42)

7mdyC Lolcde nucleotide-bound
36% identity, 77% coverage: 17:212/255 of query aligns to 21:218/226 of 7mdyC

query
sites
7mdyC

V

V

L

L

H

H

G

N

I

V

S

S

M

F

E

S

F

V

C

G

K

E

P

G

C

E

V

M

G

A

I

I

I

V

G

G

P

S

N

S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

H

C

L

I

L

N

G

V

L

L

D

K

T

P

P

K

T

S

S

G

G

W

D

V

V

C

I

L

F

D

N

G

G

Q

Q

K

P

T

M

R

S

D

K

M

L

D

S

A

S

V

A

E

A

I

K

A

A

-

E

-

L

R

R

N

N

-

Q

-

K

F

L

G

G

Y

F

V

I

P

Y

Q
|
Q

E

F

L

H

S

H

T

L

F

P

Q

D

A

F

T

T

V

A

F

L

D

E

T

N

V

V

L

A

M

M

G

-

R

-

P

P

F

L

S

L

S

I

W

G

R

K

I

-

S

-

K

K

D

K

D

P

V

A

E

E

I

I

A

N

A

S

R

R

A

A

I

L

K

E

M

M

L

L

N

K

L

A

E

V

Q

G

V

L

S

D

L

H

R

R

Y

A

N

N

-
x
H

-

R

-

P

S

S

E
|
E

L

L

S
|
S

G
|
G

Q

E

Q

R

Q

Q

R

R

V

V

M

A

I

I

A

A

R

R

A

A

L

L

A

V

Q

N

N

N

S

P

R

R

V

L

L

V

L

L

L

A

D

D

E
|
E

P

P

T

T

S

G

K
x
N

L

L

D

D

I

A

R

R

N

N

Q

A

L

D

E

S

V

I

M

F

D

Q

I

L

R

G

A

E

L

L

V

N

E

R

K

L

Q

Q

E

G

L

T

L

A

S

F

I

L

L

V

V

V

I

T

H
|
H

D

D

L

L

N

Q

L

L

A

A

S

K

R

R

Y

M

T

S

D

R

K

Q

I

L

I

E

M

M

L

R

K

D

G

G

binding adp orthovanadate: G42 (= G38), S43 (≠ A39), G44 (= G40), K45 (= K41), S46 (= S42), T47 (= T43), Q91 (= Q83), H138 (vs. gap), E142 (= E136), S144 (= S138), G145 (= G139), G146 (= G140), E168 (= E162), N172 (≠ K166), H201 (= H195)
binding magnesium ion: S46 (= S42), Q91 (= Q83)

Sites not aligning to the query:

binding adp orthovanadate: 12

P75957 Lipoprotein-releasing system ATP-binding protein LolD; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
36% identity, 77% coverage: 17:212/255 of query aligns to 24:221/233 of P75957

query
sites
P75957

V

V

L

L

H

H

G

N

I

V

S

S

M

F

E

S

F

V

C

G

K

E

P

G

C

E

V

M

G

A

I

I

I

V

G
|
G

P

S

N

S

G

G

A

S

G

G

K

K

S

S

T

T

L

L

R

H

C

L

I

L

N

G

V

L

L

D

K

T

P

P

K

T

S

S

G

G

W

D

V

V

C

I

L

F

D

N

G

G

Q

Q

K

P

T

M

R

S

D

K

M

L

D

S

A

S

V

A

E

A

I

K

A

A

-

E

-

L

R

R

N

N

-

Q

-

K

F

L

G

G

Y

F

V

I

P

Y

Q

Q

E

F

L

H

S

H

T

L

F

P

Q

D

A

F

T

T

V

A

F

L

D

E

T

N

V

V

L

A

M

M

G

-

R

-

P

P

F

L

S

L

S

I

W

G

R

K

I

-

S

-

K

K

D

K

D

P

V

A

E

E

I

I

A

N

A

S

R

R

A

A

I

L

K

E

M

M

L

L

N

K

L

A

E

V

Q

G

V

L

S

D

L

H

R

R

Y

A

N

N

-

H

-

R

-

P

S

S

E

E

L

L

S

S

G

G

Q

E

Q

R

Q

Q

R

R

V

V

M

A

I

I

A

A

R

R

A

A

L

L

A

V

Q

N

N

N

S

P

R

R

V

L

L

V

L

L

L

A

D

D

E

E

P

P

T

T

S

G

K

N

L

L

D

D

I

A

R

R

N

N

Q

A

L

D

E

S

V

I

M

F

D

Q

I

L

R

G

A

E

L

L

V

N

E

R

K

L

Q

Q

E

G

L

T

L

A

S

F

I

L

L

V

V

V

I

T

H

H

D

D

L

L

N

Q

L

L

A

A

S

K

R

R

Y

M

T

S

D

R

K

Q

I

L

I

E

M

M

L

R

K

D

G

G

G42 (= G35) mutation to D: Loss of lipoprotein release when overexpressed.

Q2G506 ATM1-type heavy metal exporter; ATP-binding cassette transporter Atm1; NaAtm1; EC 7.-.-.- from Novosphingobium aromaticivorans (strain ATCC 700278 / DSM 12444 / CCUG 56034 / CIP 105152 / NBRC 16084 / F199) (see paper)
32% identity, 85% coverage: 17:232/255 of query aligns to 376:590/608 of Q2G506

query
sites
Q2G506

V

I

L

L

H

H

G

G

I

L

S

S

M

F

E

E

F

V

C

A

K

A

P

G

C

S

V

R

V

V

G

A

I

I

I

V

G

G

P

P

N

S

G

G

A

A

G

G

K

K

S

S

T

T

L

I

L

A

R

R

C

L

I

L

N

F

N

R

V

F

L

Y

K

D

P

P

K

W

S

E

G

G

W

R

V

I

C

L

L

I

D

D

G

G

Q

Q

K

D

T

I

R

A

D

H

M

V

D

T

A

Q

V

T

E

S

I

L

A

R

R

A

N

A

F

L

G

G

Y

I

V

V

P

P

Q

Q

E

D

L

-

S

S

T

V

T

L

F

F

Q

N

A

D

T

T

V

I

F

G

D

Y

T

N

V

I

L

A

M

Y

G

G

R

R

R

D

P

G

F

-

S

-

W

-

R

-

I

A

S

S

K

R

D

A

D

E

V

V

E

D

I

A

A

A

R

K

A

G

I

A

K

A

M

I

L

A

N

D

-

F

L

I

E

A

Q

R

V

L

S

P

L

Q

R

G

Y

Y

N

D

S

T

E

E

-

V

-

G

-

E

-

R

-

G

-

L

-

K

L

L

S

S

G

G

Q

E

Q

K

Q

Q

R

R

V

V

M

A

I

I

A

A

R

R

A

T

L

L

A

V

Q

K

N

N

S

P

R

P

V

I

L

L

L

F

D

D

E
|
E

P

A

T

T

S

S

K

A

L

L

D

D

I

T

R

R

N

T

Q

E

L

Q

E

D

V

I

M

L

D

S

I

T

I

M

R

R

A

A

L

V

V

A

E

S

K

H

Q

R

E

T

L

T

L

I

S

S

I

I

L

-

V

-

I

A

H

H

D

R

L

L

N

S

L

T

A

I

S

A

R

D

Y

-

T

S

D

D

K

T

I

I

I

L

M

V

L

L

K

D

G

Q

G

G

K

R

I

L

F

A

A

E

A

Q

G

G

D

S

P

H

V

L

S

D

V

L

L

L

T

R

Q

R

D

D

N

G

I

L

R

Y

A

A

E523 (= E162) mutation to Q: Abolishes transporter activity.

Sites not aligning to the query:

195 Y→F: Strongly increases basal rate of ATP hydrolysis.
269 N→A: Increases basal rate of ATP hydrolysis and abolishes stimulation of ATP hydrolysis by glutathione.
269:272 binding glutathione
272 Q→A: Abolishes glutathione-dependent ATP hydrolysis.
316:319 binding glutathione
319 G→L: Abolishes glutathione-dependent ATP hydrolysis.

4mrvA Structure of a bacterial atm1-family abc transporter (see paper)
32% identity, 85% coverage: 17:232/255 of query aligns to 369:583/600 of 4mrvA

query
sites
4mrvA

V

I

L

L

H

H

G

G

I

L

S

S

M

F

E

E

F

V

C

A

K

A

P

G

C

S

V

R

V

V

G

A

I

I

I

V

G

G

P

P

N
x
S

G

G

A

A

G
|
G

K

K

S
|
S

T

T

L

I

L

A

R

R

C

L

I

L

N

F

N

R

V

F

L

Y

K

D

P

P

K

W

S

E

G

G

W

R

V

I

C

L

L

I

D

D

G

G

Q

Q

K

D

T

I

R

A

D

H

M

V

D

T

A

Q

V

T

E

S

I

L

A

R

R

A

N

A

F

L

G

G

Y

I

V

V

P

P

Q

Q

E

D

L

-

S

S

T

V

T

L

F

F

Q

N

A

D

T

T

V

I

F

G

D

Y

T

N

V

I

L

A

M

Y

G

G

R

R

R

D

P

G

F

-

S

-

W

-

R

-

I

A

S

S

K

R

D

A

D

E

V

V

E

D

I

A

A

A

R

K

A

G

I

A

K

A

M

I

L

A

N

D

-

F

L

I

E

A

Q

R

V

L

S

P

L

Q

R

G

Y

Y

N

D

S

T

E

E

-

V

-

G

-

E

-

R

-

G

-

L

-

K

L

L

S

S

G

G

Q

E

Q

K

Q

Q

R

R

V

V

M

A

I

I

A

A

R

R

A

T

L

L

A

V

Q

K

N

N

S

P

R

P

V

I

L

L

L

F

D

D

E

E

P

A

T

T

S

S

K

A

L

L

D

D

I

T

R

R

N

T

Q

E

L

Q

E

D

V

I

M

L

D

S

I

T

I

M

R

R

A

A

L

V

V

A

E

S

K

H

Q

R

E

T

L

T

L

I

S

S

I

I

L

-

V

-

I

A

H

H

D

R

L

L

N

S

L

T

A

I

S

A

R

D

Y

-

T

S

D

D

K

T

I

I

I

L

M

V

L

L

K

D

G

Q

G

G

K

R

I

L

F

A

A

E

A

Q

G

G

D

S

P

H

V

L

S

D

V

L

L

L

T

R

Q

R

D

D

N

G

I

L

R

Y

A

A

binding phosphate ion: S389 (≠ N37), G392 (= G40), S394 (= S42)

Sites not aligning to the query:

binding mercury bis(L-gamma-glutamyl-3-sulfido-L-alanylglycine): 149, 261, 262, 265, 309, 310, 311, 312, 313
binding phosphate ion: 313, 316

4mrsA Structure of a bacterial atm1-family abc transporter (see paper)
32% identity, 85% coverage: 17:232/255 of query aligns to 369:583/600 of 4mrsA

query
sites
4mrsA

V

I

L

L

H

H

G

G

I

L

S

S

M

F

E

E

F

V

C

A

K

A

P

G

C

S

V

R

V

V

G

A

I

I

I

V

G

G

P

P

N
x
S

G

G

A

A

G
|
G

K

K

S
|
S

T

T

L

I

L

A

R

R

C

L

I

L

N

F

N

R

V

F

L

Y

K

D

P

P

K

W

S

E

G

G

W

R

V

I

C

L

L

I

D

D

G

G

Q

Q

K

D

T

I

R

A

D

H

M

V

D

T

A

Q

V

T

E

S

I

L

A

R

R

A

N

A

F

L

G

G

Y

I

V

V

P

P

Q

Q

E

D

L

-

S

S

T

V

T

L

F

F

Q

N

A

D

T

T

V

I

F

G

D

Y

T

N

V

I

L

A

M

Y

G

G

R

R

R

D

P

G

F

-

S

-

W

-

R

-

I

A

S

S

K

R

D

A

D

E

V

V

E

D

I

A

A

A

R

K

A

G

I

A

K

A

M

I

L

A

N

D

-

F

L

I

E

A

Q

R

V

L

S

P

L

Q

R

G

Y

Y

N

D

S

T

E

E

-

V

-

G

-

E

-

R

-

G

-

L

-

K

L

L

S

S

G

G

Q

E

Q

K

Q

Q

R

R

V

V

M

A

I

I

A

A

R

R

A

T

L

L

A

V

Q

K

N

N

S

P

R

P

V

I

L

L

L

F

D

D

E

E

P

A

T

T

S

S

K

A

L

L

D

D

I

T

R

R

N

T

Q

E

L

Q

E

D

V

I

M

L

D

S

I

T

I

M

R

R

A

A

L

V

V

A

E

S

K

H

Q

R

E

T

L

T

L

I

S

S

I

I

L

-

V

-

I

A

H

H

D

R

L

L

N

S

L

T

A

I

S

A

R

D

Y

-

T

S

D

D

K

T

I

I

I

L

M

V

L

L

K

D

G

Q

G

G

K

R

I

L

F

A

A

E

A

Q

G

G

D

S

P

H

V

L

S

D

V

L

L

L

T

R

Q

R

D

D

N

G

I

L

R

Y

A

A

binding phosphate ion: S389 (≠ N37), G392 (= G40), S394 (= S42)

Sites not aligning to the query:

binding oxidized glutathione disulfide: 142, 149, 199, 203, 261, 262, 265, 309, 310, 312, 313

Query Sequence

>WP_048051364.1 NCBI__GCF_000744315.1:WP_048051364.1
MRLSIDGVKLSYASVPVLHGISMEFCKPCVVGIIGPNGAGKSTLLRCINNVLKPKSGWVC
LDGQKTRDMDAVEIARNFGYVPQELSTTFQATVFDTVLMGRRPFSSWRISKDDVEIAARA
IKMLNLEQVSLRYNSELSGGQQQRVMIARALAQNSRVLLLDEPTSKLDIRNQLEVMDIIR
ALVEKQELLSILVIHDLNLASRYTDKIIMLKGGKIFAAGDPVSVLTQDNIRAVYDVEAAV
VLEDNLPHIIPKRPV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory