SitesBLAST

P16250 Phosphoribosyl isomerase A; 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase; N-(5'-phosphoribosyl)anthranilate isomerase; PRAI; Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase; EC 5.3.1.16; EC 5.3.1.24 from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) (see paper)
36% identity, 96% coverage: 7:241/246 of query aligns to 3:237/240 of P16250

query
sites
P16250

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D11 (= D15) mutation to A: No activity.
R19 (≠ Q23) mutation to A: No effect on activity toward PRA. No activity toward ProFAR.
S81 (≠ G86) mutation to T: No activity toward PRA. Almost no effect on activity toward ProFAR.
D130 (= D136) mutation to A: Very low activity toward PRA. No activity toward ProFAR.; mutation to Q: No activity.
T166 (= T173) mutation to A: No activity.
D171 (≠ E178) mutation to A: Low activity toward PRA. No activity toward ProFAR.

4tx9A Crystal structure of hisap from streptomyces sviceus with degraded profar (see paper)
37% identity, 96% coverage: 7:241/246 of query aligns to 8:242/246 of 4tx9A

query
sites
4tx9A

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active site: D16 (= D15), D135 (= D136)
binding aminoimidazole 4-carboxamide ribonucleotide: G28 (= G27), V57 (= V57), L59 (= L59), S86 (≠ G86), G88 (= G88), R90 (= R90), G109 (= G109), T110 (= T110), D135 (= D136), G145 (= G146), W146 (= W147)

3zs4A Crystal structure of mycobacterium tuberculosis phosphoribosyl isomerase with bound prfar
35% identity, 95% coverage: 8:240/246 of query aligns to 3:239/244 of 3zs4A

query
sites
3zs4A

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active site: D10 (= D15), D129 (= D136)
binding phosphoric acid mono-[5-({[5-carbamoyl-3-(5-phosphonooxy-5-deoxy-ribofuranosyl)- 3h-imidazol-4-ylamino]-methyl}-amino)-2,3,4-trihydroxy-pentyl] ester: A8 (= A13), D10 (= D15), R18 (≠ Q23), A56 (= A62), F57 (≠ L63), S80 (≠ G86), G82 (= G88), R84 (= R90), G103 (= G109), T104 (= T110), D129 (= D136), G143 (= G146), W144 (= W147), D174 (≠ E178), G175 (= G179), G200 (= G204), G201 (= G205), I223 (≠ V224), G225 (= G226), K226 (≠ S227)

2y88A Crystal structure of mycobacterium tuberculosis phosphoribosyl isomerase (variant d11n) with bound prfar (see paper)
35% identity, 95% coverage: 8:240/246 of query aligns to 3:239/244 of 2y88A

query
sites
2y88A

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active site: N10 (≠ D15), D129 (= D136)
binding [(2r,3s,4r,5r)-5-[4-aminocarbonyl-5-[[(z)-[(3r,4r)-3,4-dihydroxy-2-oxo-5-phosphonooxy-pentyl]iminomethyl]amino]imidazol-1-yl]-3,4-dihydroxy-oxolan-2-yl]methyl dihydrogen phosphate: A8 (= A13), N10 (≠ D15), R18 (≠ Q23), G22 (= G27), L53 (= L59), A56 (= A62), S80 (≠ G86), G81 (= G87), G82 (= G88), R84 (= R90), G103 (= G109), T104 (= T110), D129 (= D136), G143 (= G146), W144 (= W147), D174 (≠ E178), G175 (= G179), S199 (= S203), G200 (= G204), G225 (= G226), K226 (≠ S227)

5abtA S.Enterica hisa mutant d7n, g102a, v106m, d176a
30% identity, 97% coverage: 9:246/246 of query aligns to 1:246/246 of 5abtA

query
sites
5abtA

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A

V

F

I

Q

Y

S

S
|
S

G
|
G

G

G

V

I

S

G

S

D

L

I

N

D

D

D

L

I

R

A

M

A

L

L

Q

R

G

G

T

T

G

G

V

V

M

R

G

G

V

V

V
x
I

I

V

G
|
G

S
x
R

A

A

I

L

Y

L

R

E

G

G

L

K

I

F

D

T

L

V

T

K

E

E

A

A

L

I

K

Q

-

C

Y

W

Q

Q

D

N

L

V

K

K

active site: N7 (≠ D15), D129 (= D136)
binding [(2R,3S,4R,5R)-5-[4-aminocarbonyl-5-[(E)-[[(2R,3R,4S,5R)-3,4-bis(oxidanyl)-5-(phosphonooxymethyl)oxolan-2-yl]amino]methylideneamino]imidazol-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl dihydrogen phosphate: N7 (≠ D15), G19 (= G27), V49 (= V57), L51 (= L59), G81 (= G88), R83 (= R90), V100 (≠ I107), A102 (≠ G109), S103 (≠ T110), D129 (= D136), G144 (= G146), W145 (= W147), G177 (= G179), S202 (= S203), G203 (= G204), I223 (≠ V224), G225 (= G226), R226 (≠ S227)

2y85A Crystal structure of mycobacterium tuberculosis phosphoribosyl isomerase with bound rcdrp (see paper)
33% identity, 95% coverage: 8:240/246 of query aligns to 3:230/234 of 2y85A

query
sites
2y85A

M

L

L

I

I

L

P

P

A

A

V

V

D
|
D

I

V

K

V

D

E

G

G

K

R

C

A

V

V

Q

R

L

T

V

E

Q

Y

G

G

K

S

P

-

G

-

T

-

E

-

Q

-

V

-

V

A

L

V

D

D

D

A

P

A

A

L

G

G

V

-

A

-

G

-

R

-

W

W

E

Q

S

R

L

D

G

G

A

A

E

E

T

W

I

I

H
|
H

V

L

V
|
V

D

D

L
|
L

D

D

G

A

A
|
A

L

F

G

G

L

R

E

G

K

S

N

N

T

H

G

E

I

L

L

L

K

A

E

E

I

V

T

V

E

G

R

K

V

L

S

D

V

V

P

Q

L

V

Q

E

I

L

G
x
S

G

G

I

I

R

R

S

D

R

D

E

E

Y

S

A

L

G

A

K

A

L

A

L

L

D

A

M

T

G

G

F

C

E

A

R

R

V

V

I

N

L

V

G

G

T

T

M

A

A

A

I

L

E

E

N

N

P

P

A

Q

I

W

V

C

E

A

E

R

L

V

A

I

G

G

E

E

Y

H

G

G

S

-

E

D

R

Q

I

V

M

A

V

V

S

G

L

L

D
|
D

S

V

R

Q

-

I

-

D

-

G

D

E

S

H

R

R

V

L

V

R

I

G

R
|
R

G

G

W

W

T

-

E

E

K

T

V

D

P

G

F

G

T

D

A

L

E

W

E

D

M

V

A

L

R

E

K

R

F

L

Q

D

E

S

R

E

G

G

A

C

G

S

S

R

I

F

L

V

F

V

T

T

N

D

V

I

D

T

F

K

E
x
D

G
|
G

L

T

L

L

S

G

G

G

F

P

D

N

L

L

K

D

P

L

V

L

S

A

E

G

L

V

V

A

E

D

A

R

V

T

E

D

I

A

P

P

V

V

I

I

Y

A

S
|
S

G
|
G

V

V

S

S

L

L

N

D

D

D

L

L

R

R

-

A

-

I

-

A

M

T

L

L

Q

T

G

H

T

R

G

G

V

V

M

E

G

G

V

A

V

I

I

V

G
|
G

S
x
K

A

A

I

L

Y

Y

R

A

G

R

L

R

I

F

D

T

L

L

T

P

E

Q

A

A

L

L

active site: D10 (= D15), D120 (= D136)
binding 1-(o-carboxy-phenylamino)-1-deoxy-d-ribulose-5-phosphate: D10 (= D15), H40 (= H55), V42 (= V57), L44 (= L59), A47 (= A62), S71 (≠ G86), R133 (= R145), D165 (≠ E178), G166 (= G179), S190 (= S203), G191 (= G204), G192 (= G205), G216 (= G226), K217 (≠ S227)

Q9X0C7 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase; Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase; EC 5.3.1.16 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
29% identity, 96% coverage: 8:242/246 of query aligns to 1:239/241 of Q9X0C7

query
sites
Q9X0C7

M

M

L

L

I

V

P

P

A

A

V

I

D
|
D

I

L

K

F

D

R

G

G

K

K

C

V

V

A

Q

R

L

M

V

I

Q

K

G

G

K

R

P

K

G

E

T

N

E

T

Q

I

V

F

V

Y

L

E

D

K

D

D

P

P

A

V

G

E

V

L

A

V

G

E

R

K

W

L

E

I

S

E

L

E

G

G

A

F

E

T

T

L

I

I

H
|
H

V

V

D
|
D

L

L

D

S

G

N

A

A

L

I

G

E

L

N

E

S

-

G

K

E

N

N

T

L

G

P

I

V

L

L

K

E

E

K

I

L

T

S

E

E

R

-

V

F

S

A

V

E

P

H

L

I

Q

Q

I

I

G

G

I

I

R
|
R

S

S

R

L

E

D

Y

Y

A

A

G

E

K

K

L

L

L

R

D

K

M

L

G

G

F

Y

E

R

R

R

V

Q

I

I

L

V

G

S

T

S

M

K

A

V

I

L

E

E

N

D

P

P

A

S

I

F

V

L

E

K

E

S

L

L

A

R

G

-

E

E

Y

I

G

D

S

V

E

E

R

P

I

V

M

F

V

-

S

S

L

L

D
|
D

S

T

R

R

D

G

S

G

R

R

V

V

V

A

I

F

R

K

G

G

W

W

T

L

E

A

K

E

V

E

P

E

F

I

T

D

A

P

E

V

E

S

M

L

A

L

R

K

K

R

F

L

Q

K

E

E

R

Y

G

G

A

L

G

E

S

E

I

I

L

V

F

H

T
|
T

N

E

V

I

D

E

F

K

E

D

G

G

L

T

L

L

S

Q

G

E

F

H

D

D

L

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K

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P

L

V

T

S

K

E

K

L

I

V

A

E

I

A

E

V

A

E

E

I

V

P

K

V

V

I

L

Y

A

S

A

G

G

V

I

S

S

L

E

N

N

D

S

L

L

R

K

M

T

L

A

Q

Q

G

K

T

V

G

H

-

T

-

E

-

T

-

N

-

G

-

L

V

L

M

K

G

G

V

V

V

I

I

V

G

G

S

R

A

A

I

F

Y

L

R

E

G

G

L

I

I

L

D

T

L

V

T

E

E

V

A

M

L

K

K

R

Y

Y

D8 (= D15) mutation to N: Loss of activity.
H48 (= H55) mutation to A: Decrease in activity.
D51 (= D58) mutation to N: Decrease in activity.
R83 (= R90) mutation to N: Decrease in activity.
D127 (= D136) mutation to N: Almost no activity.
T164 (= T173) mutation to A: Strong decrease in activity.

1h5yB Hisf protein from pyrobaculum aerophilum (see paper)
34% identity, 83% coverage: 8:212/246 of query aligns to 5:213/253 of 1h5yB

query
sites
1h5yB

M

L

L

R

I

I

P

P

A

C

V

L

D
|
D

I

I

K

-

D

D

G

G

K

G

C

A

V

K

Q

V

L

V

V

V

Q
x
K

G

G

K

V

P

N

G

F

T

Q

E

G

Q

I

V

R

V

E

L

V

D

G

D

D

P

P

A

V

G

E

V

M

A

A

G

V

R

R

W

Y

E

E

S

E

L

E

G

G

A

A

E

D

T

E

I

I

H

A

V

I

V

L

D

D

L

I

D

T

G

A

A

A

L

P

-

E

G

G

L

R

E

A

K

T

N

F

T

I

G

D

I

S

L

V

K

K

E

R

I

V

T

A

E

E

R

A

V

V

S

S

V

I

P

P

L

V

Q

L

I

V

G

G

G
|
G

I

V

R

R

S

S

R

L

E

E

Y

D

A

A

G

T

K

T

L

L

L

F

D

R

M

A

G

G

F

A

E

D

R

K

V

V

I

S

L

V

G
x
N

T
|
T

M

A

A

A

I

V

E

R

N

N

P

P

A

Q

I

L

V

V

E

A

E

L

L

L

A

A

G

R

E

E

Y

F

G

G

S

S

E

Q

R

S

I

T

M

V

V

V

S
x
A

L

I

D
|
D

S

A

R

K

D

W

S

N

-

G

-

E

-

Y

R

E

V

V

V

Y

I

V

R

K

G
|
G

W

G

T

R

E

E

K

A

V

T

P

G

F

L

T

D

A

A

E

V

E

K

M

W

A

A

R

K

K

E

F

V

Q

E

E

E

R

L

G

G

A

A

G

G

S

E

I

I

L

L

F

L

T
|
T

N

S

V

I

D

D

F

R

E
x
D

G
|
G

L

T

L

G

S

L

G

G

F

Y

D

D

L

V

K

E

P

L

V

I

S

R

E

R

L

V

V

A

E

D

A

S

V

V

E

R

I

I

P

P

V

V

I

I

Y

A

S

S

G

G

V

A

S

G

S

R

L

V

N

E

D

H

L

F

active site: D12 (= D15), D133 (= D136)
binding glycerol: K22 (≠ Q26), N106 (≠ G109), A131 (≠ S134), D133 (= D136), G147 (= G146), T174 (= T173), D179 (≠ E178)
binding phosphate ion: G84 (= G87), G85 (= G88), N106 (≠ G109), T107 (= T110), D179 (≠ E178), G180 (= G179)

Sites not aligning to the query:

binding phosphate ion: 226, 227, 228

5ab3A S.Enterica hisa mutant d7n, d10g, dup13-15, q24l, g102a (see paper)
28% identity, 95% coverage: 9:241/246 of query aligns to 1:237/241 of 5ab3A

query
sites
5ab3A

L

M

I

I

P

P

A

A

V

L

D
x
N

I

L

K

I

D

G

G

G

K

T

C

V

V

V

Q

R

L

V

V

V

Q
x
R

G

L

K

H

P

Q

G

G

T

D

E
x
Y

Q

A

V

R

V

L

L

R

D

D

-

Y

-

G

-

N

D

D

P

P

A

L

G

P

V

R

A

L

G

Q

R

D

W

Y

E

A

S

A

L

Q

G

G

A

A

E

G

T

V

I

L

H

H

V

L

V
|
V

D

D

L

L

D

T

G

G

A

A

L

K

G

D

-

P

L

A

E

K

K

R

N

Q

T

I

G

P

I

L

L

I

K

K

E

T

I

L

T

V

E

A

R

G

V

V

S

N

V

V

P

P

L

V

Q

Q

I

V

G

G

G
|
G

I

V

R

R

S

T

R

E

E

E

Y

D

A

V

G

A

K

A

L

L

D

K

M

A

G

G

F

V

E

A

R

R

V

V

I

V

L

I

G
x
A

T
x
S

M

T

A

A

I

V

E

K

N

S

P

P

A

D

I

V

V

V

E

K

E

G

L

W

A

F

G

E

E

R

Y

F

G

G

S

A

E

Q

R

A

I

L

M

V

V

L

S
x
A

L

L

D
|
D

S

V

R

R

D

-

S

-

R

-

V

-

V

I

I

D

R

E

G

H

W

G

T

T

E

K

K

Q

V

V

P

A

F

V

T

S

A

G

-

V

-

S

-

L

E

E

E

Q

M

L

A

V

R

E

K

T

F

Y

Q

L

E

P

R

V

G

G

A

L

G

K

S

H

I

V

L

L

F

C

T

T

N

D

V

I

D

S

F

R

E

D

G
|
G

L

T

L

L

S

A

G

G

F

S

D

N

L

V

K

S

P

L

V

Y

S

E

E

E

L

V

V

C

E

A

A

R

V

Y

-

P

E

Q

I

I

P

A

V

F

I

Q

Y

S

S
|
S

G
|
G

V

I

S

G

S

D

L

I

N

D

D

D

L

I

R

A

M

A

L

L

Q

R

G

G

T

T

G

G

V

V

M

R

G

G

V

V

V

I

I

V

G
|
G

S
x
R

A

A

I

L

Y

L

R

E

G

G

L

K

I

F

D

T

L

V

T

K

E

E

A

A

L

I

K

Q

active site: N7 (≠ D15), D132 (= D136)
binding [(2r,3s,4r,5r)-5-[4-aminocarbonyl-5-[[(z)-[(3r,4r)-3,4-dihydroxy-2-oxo-5-phosphonooxy-pentyl]iminomethyl]amino]imidazol-1-yl]-3,4-dihydroxy-oxolan-2-yl]methyl dihydrogen phosphate: N7 (≠ D15), R18 (≠ Q26), Y24 (≠ E32), V52 (= V57), G84 (= G88), A105 (≠ G109), S106 (≠ T110), A130 (≠ S134), D132 (= D136), G174 (= G179), S199 (= S203), G200 (= G204), G201 (= G205), G222 (= G226), R223 (≠ S227)

P60664 Imidazole glycerol phosphate synthase subunit HisF; IGP synthase cyclase subunit; IGP synthase subunit HisF; ImGP synthase subunit HisF; IGPS subunit HisF; EC 4.3.2.10 from Escherichia coli (strain K12) (see paper)
25% identity, 95% coverage: 10:242/246 of query aligns to 6:247/258 of P60664

query
sites
P60664

I

I

P

P

A

C

V

L

D

D

I

V

K

R

D

D

G

G

K

-

C

-

V

-

Q

Q

L

V

V

V

Q

K

G

G

K

V

P

Q

G

F

T

R

E

N

Q

H

V

E

V

I

L

I

D

G

D

D

P

I

A

V

G

P

V

L

A

A

G

K

R

R

W

Y

E

A

S

E

L

E

G

G

A

A

E

D

T
x
E

I

L

H

V

V

F

V

Y

D

D

L

I

D

T

G

A

A

S

L

S

-

D

G

G

L

R

E

V

K

V

N

D

T

K

G

S

I

W

L

V

K

S

E

R

I

V

T

A

E

E

R

V

V

I

S

D

V

I

P

P

L

F

Q

C

I

V

G

A

G

G

I

I

R

K

S

S

R

L

E

E

Y

D

A

A

G

A

K

K

L

I

L

L

D

S

M

F

G

G

F

A

E

D

R

K

V

I

I

S

L

I

G

N

T

S

M

P

A

A

I

L

E

A

N

D

P

P

A

T

I

L

V

I

E

T

E

R

L

L

A

A

G

D

E

R

Y

F

G

G

S

V

E
x
Q

R
x
C

I

I

M

V

V

V

S

G

L

I

D

D

S

T

-

W

-

Y

-

D

-

A

-

E

-

T

-

G

-

K

-

Y

-

H

-

V

-

N

-

Q

-

Y

-

T

-

G

R

D

D

E

S

S

R

R

V

T

V

R

I

V

R

T

G

Q

W

W

T

-

E

E

K

T

V

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P

D

F

W

T

V

A

Q

E

E

E

-

M

-

A

-

R

-

K

-

F

V

Q

Q

E

K

R

R

G

G

A

A

G

G

S

E

I

I

L

V

F

L

T

N

N

M

V

M

D

N

F

Q

E

D

G

G

L

V

L

R

S

N

G

G

F

Y

D

D

L

L

K

E

P

Q

V

L

S

K

E

K

L

V

V

R

E

E

A

V

V

C

E

H

I

V

P

P

V

L

I

I

Y

A

S

S

G

G

V

A

S

G

S

T

L

M

N

E

D

H

-

F

L

L

R

E

M

A

L

F

Q

R

G

D

T

A

G

D

V

V

M

D

G

G

V

A

V

L

I

A

G

A

S

S

A

V

I

F

Y

H

R

K

G

Q

L

I

I

I

D

N

L

I

T

G

E

E

A

L

L

K

K

A

Y

Y

E46 (≠ T53) mutation to A: Loss of activity.; mutation to G: Loss of IGP synthase activity. Weak IGP synthase and HisH activities in vitro.
Q123 (≠ E129) mutation to A: Decrease in activity.; mutation to R: Loss of IGP synthase activity. Weak HisH activity in vitro.
C124 (≠ R130) mutation to A: No change in activity.; mutation to R: Loss of IGP synthase activity. Weak HisH activity in vitro.

Sites not aligning to the query:

5 R→A: Loss of activity.; R→H: Loss of IGP synthase activity. Weak IGP synthase activity and reduced HisH activity in vitro.

3zr4E Structural evidence for ammonia tunneling across the (beta-alpha)8 barrel of the imidazole glycerol phosphate synthase bienzyme complex (see paper)
25% identity, 83% coverage: 10:212/246 of query aligns to 6:201/244 of 3zr4E

query
sites
3zr4E

I

I

P

A

A

C

V

L

D
|
D

I

V

K

K

D

D

G

G

K

R

C

V

V

V

Q

-

L

-

V

-

Q

-

G

-

K

K

P

S

G

G

T

-

E

-

Q

-

V

-

L

-

D

-

D

D

P

P

A

V

G

E

V

L

A

G

G

K

R

F

W

Y

E

S

S

E

L

I

G

G

A

I

E

D

T

E

I

L

H

V

V

F

V

L

D

D

L

I

D

T

G

A

A

S

L

V

G

E

L

K

E

R

K

K

N

T

T

M

-

L

G

E

I

L

L

V

K

E

E

K

I

V

T

A

E

E

R

Q

V

I

S

D

V

I

P

P

L

F

Q

T

I

V

G

G

I

I

R

H

S

D

R

F

E

E

Y

T

A

A

G

S

K

E

L

L

L

I

D

L

M

R

G

G

F

A

E

D

R

K

V

V

I

S

L

I

G

N

T

T

M

A

A

A

I

V

E

E

N

N

P

P

A

S

I

L

V

I

E

T

E

Q

L

I

A

A

G

Q

E

T

Y

F

G

G

S

S

E

Q

R

A

I

V

M

V

V

V

S

A

L

I

D
|
D

S

A

-

K

-

R

-

V

-

D

-

G

-

E

-

F

-

M

-

V

-

F

-

T

-

Y

-

S

-

G

-

K

R

K

D

N

S

T

R

G

V

I

V

L

I

L

R

R

G

D

W

W

T

V

E

V

K

E

V

V

P

-

F

-

T

-

A

-

E

-

M

-

A

-

R

-

K

-

F

-

Q

E

E

K

R

R

G

G

A

A

G

G

S

E

I

I

L

L

F

L

T

T

N

S

V

I

D

D

F

R

E

D

G

G

L

T

L

K

S

S

G

G

F

Y

D

D

L

T

K

E

P

M

V

I

S
x
R

E

F

L

V

V

R

E

P

A

L

V

T

E

T

I

L

P

P

V

I

I

I

Y

A

S

S

G

G

V

A

S

G

S

K

L

M

N

E

D

H

L

F

active site: D11 (= D15), D121 (= D136)
binding glycerol: R179 (≠ S190)

Sites not aligning to the query:

binding glycerol: 206

7qc8A Imidazole glycerol phosphate synthase subunit HisF (see paper)
27% identity, 83% coverage: 10:212/246 of query aligns to 6:210/250 of 7qc8A

query
sites
7qc8A

I

I

P

A

A

A

V

L

D
x
E

I

V

K

K

D

D

G

G

K

R

C

V

V

V

Q

K

L

-

V

-

Q

-

G

-

K

-

P

-

G

G

T

T

E

N

Q

F

V

E

V

N

L

L

D

R

D

D

-

S

-

G

-

D

P

P

A

A

G

E

V

L

A

G

G

K

R

F

W

Y

E

S

S

E

L

I

G

G

A

I

E

D

T

E

I

L

H

-

V

-

V

V

D

F

L
x
H

D

D

G
x
H

A

T

L

A

G

S

L

V

E

E

K

K

N

R

T

K

G

T

I

M

L

L

K

E

-

L

-

V

-

E

E

K

I

V

T

A

E

E

R

Q

V

I

S

D

V

I

P

P

L

F

Q

T

I

V

G

G

I

I

R

H

S

D

R

F

E

E

Y

T

A

A

G

S

K

E

L

L

L

I

D

L

M

R

G

G

F

A

E

D

R

K

V

V

I

S

L

I

G

N

T

T

M

A

A

A

I

V

E

E

N

N

P

P

A

S

I

L

V

I

E

T

E

Q

L

I

A

A

G

Q

E

T

Y

F

G

G

S

S

E

Q

R

A

I

V

M

V

V

V

S

A

L

I

D

D

S

A

-

K

-

R

-

V

-

D

-

G

-

E

-

F

-

M

-

V

-

F

-

T

-

Y

-

S

-

G

-

K

R

K

D

N

S

T

R

G

V

I

V

L

I

L

R

R

G

D

W

W

T

V

E

V

K

E

V

V

P

-

F

-

T

-

A

-

E

-

M

-

A

-

R

-

K

-

F

-

Q

E

E

K

R

R

G

G

A

A

G

G

S

E

I

I

L

L

F

L

T

T

N

S

V

I

D

D

F

R

E

D

G

G

L

T

L

K

S

S

G

G

F

Y

D

D

L

T

K

E

P

M

V

I

S

R

E

F

L

V

V

R

E

P

A

L

V

T

E

T

I

L

P

P

V

I

I

I

Y

A

S

S

G

G

V

A

S

G

S

K

L

M

N

E

D

H

L

F

binding zinc ion: E11 (≠ D15), H50 (≠ L59), H52 (≠ G61)

7ac8A Molecular basis for the unique allosteric activation mechanism of the heterodimeric imidazole glycerol phosphate synthase complex. (see paper)
25% identity, 83% coverage: 10:212/246 of query aligns to 6:210/252 of 7ac8A

query
sites
7ac8A

I

I

P

A

A

C

V

L

D
|
D

I

V

K

K

D

D

G

G

K

R

C

V

V

V

Q

K

L

-

V

-

Q

-

G

-

K

-

P

-

G

G

T

T

E

N

Q

F

V

E

V

N

L

L

D

R

D

D

-

S

-

G

-

D

P

P

A

V

G

E

V

L

A

G

G

K

R

F

W

Y

E

S

S

E

L

I

G

G

A

I

E

D

T

E

I

L

H

V

V

F

V
x
L

D

D

L
x
I

D

T

G

A

A

S

L

V

G

E

L

K

E

R

K

K

N

T

T

M

-

L

G

E

I

L

L

V

K

E

E

K

I

V

T

A

E

E

R

Q

V

I

S

D

V

I

P

P

L

F

Q

T

I

V

G

G

G
|
G

I

I

R

H

S

D

R

F

E

E

Y

T

A

A

G

S

K

E

L

L

L

I

D

L

M

R

G

G

F

A

E

D

R

K

V

V

I

S

L

I

G
x
N

T
|
T

M

A

A

A

I

V

E

E

N

N

P

P

A

S

I

L

V

I

E

T

E

Q

L

I

A

A

G

Q

E

T

Y

F

G

G

S

S

E

Q

R

A

I

V

M

V

V

V

S

A

L

I

D
|
D

S

A

-

K

-

R

-

V

-

D

-

G

-

E

-

F

-

M

-

V

-

F

-

T

-

Y

-
x
S

-

G

-

K

R

K

D

N

S

T

R

G

V

I

V

L

I

L

R

R

G

D

W

W

T

V

E

V

K

E

V

V

P

-

F

-

T

-

A

-

E

-

M

-

A

-

R

-

K

-

F

-

Q

E

E

K

R

R

G

G

A

A

G

G

S

E

I

I

L

L

F

L

T

T

N

S

V

I

D

D

F

R

E
x
D

G
|
G

L

T

L

K

S

S

G

G

F

Y

D

D

L

T

K

E

P

M

V

I

S

R

E

F

L

V

V

R

E

P

A

L

V

T

E

T

I

L

P

P

V

I

I

I

Y

A

S
|
S

G

G

V

A

S

G

S

K

L

M

N

E

D

H

L

F

active site: D11 (= D15), D130 (= D136)
binding [(2R,3S,4R,5R)-5-[4-aminocarbonyl-5-[(E)-[[(2R,3R,4S,5R)-3,4-bis(oxidanyl)-5-(phosphonooxymethyl)oxolan-2-yl]amino]methylideneamino]imidazol-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl dihydrogen phosphate: L50 (≠ V57), I52 (≠ L59), G82 (= G88), N103 (≠ G109), T104 (= T110), D130 (= D136), S144 (vs. gap), D176 (≠ E178), G177 (= G179), S201 (= S203)

Sites not aligning to the query:

binding [(2R,3S,4R,5R)-5-[4-aminocarbonyl-5-[(E)-[[(2R,3R,4S,5R)-3,4-bis(oxidanyl)-5-(phosphonooxymethyl)oxolan-2-yl]amino]methylideneamino]imidazol-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl dihydrogen phosphate: 225

Q9X0C6 Imidazole glycerol phosphate synthase subunit HisF; IGP synthase cyclase subunit; IGP synthase subunit HisF; ImGP synthase subunit HisF; IGPS subunit HisF; EC 4.3.2.10 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
25% identity, 83% coverage: 10:212/246 of query aligns to 6:210/253 of Q9X0C6

query
sites
Q9X0C6

I

I

P

A

A
x
C

V

L

D
|
D

I

V

K

K

D

D

G

G

K

R

C

V

V

V

Q
x
K

L

-

V

-

Q

-

G

-

K

-

P

-

G

G

T

T

E

N

Q

F

V

E

V

N

L

L

D

R

D

D

-

S

-

G

-

D

P

P

A

V

G

E

V

L

A

G

G

K

R

F

W

Y

E

S

S

E

L

I

G

G

A

I

E

D

T

E

I

L

H

V

V

F

V

L

D
|
D

L

I

D

T

G

A

A

S

L

V

G

E

L

K

E

R

K

K

N

T

T

M

-

L

G

E

I

L

L

V

K

E

E

K

I

V

T

A

E

E

R

Q

V

I

S

D

V

I

P

P

L

F

Q

T

I

V

G

G

I

I

R

H

S

D

R

F

E

E

Y

T

A

A

G

S

K

E

L

L

L

I

D

L

M

R

G

G

F

A

E

D

R

K

V

V

I

S

L

I

G
x
N

T

T

M

A

A

A

I

V

E

E

N

N

P

P

A

S

I

L

V

I

E

T

E

Q

L

I

A

A

G

Q

E

T

Y

F

G

G

S

S

E

Q

R

A

I

V

M

V

V

V

S

A

L

I

D
|
D

S

A

-

K

-

R

-

V

-

D

-

G

-

E

-

F

-

M

-

V

-

F

-

T

-

Y

-

S

-

G

-

K

R

K

D

N

S

T

R

G

V

I

V

L

I

L

R

R

G

D

W

W

T

V

E

V

K

E

V

V

P

-

F

-

T

-

A

-

E

-

M

-

A

-

R

-

K

-

F

-

Q

E

E

K

R

R

G

G

A

A

G

G

S

E

I

I

L

L

F

L

T

T

N

S

V

I

D

D

F

R

E
x
D

G

G

L

T

L

K

S

S

G

G

F

Y

D
|
D

L

T

K

E

P

M

V

I

S

R

E

F

L

V

V

R

E

P

A

L

V

T

E

T

I

L

P

P

V

I

I

I

Y

A

S

S

G

G

V

A

S

G

S

K

L

M

N

E

D

H

L

F

C9 (≠ A13) mutation to A: No change in activity.
D11 (= D15) mutation to X: Loss of activity.
K19 (≠ Q23) mutation to S: Decrease in activity.
D51 (= D58) mutation to N: No change in activity.
N103 (≠ G109) mutation to A: No change in activity.
D130 (= D136) mutation D->A,C,F,G,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y: Complete loss of activity.; mutation to E: Weak activity.
D176 (≠ E178) mutation to N: Decrease in activity.
D183 (= D185) mutation to N: No change in activity.

1gpwC Structural evidence for ammonia tunneling across the (beta/alpha)8 barrel of the imidazole glycerol phosphate synthase bienzyme complex. (see paper)
25% identity, 83% coverage: 10:212/246 of query aligns to 6:210/253 of 1gpwC

query
sites
1gpwC

I

I

P

A

A

C

V

L

D
x
N

I

V

K

K

D

D

G

G

K

R

C

V

V

V

Q

K

L

-

V

-

Q

-

G

-

K

-

P

-

G

G

T

T

E

N

Q

F

V

E

V

N

L

L

D

R

D

D

-

S

-

G

-

D

P

P

A

V

G

E

V

L

A

G

G

K

R

F

W

Y

E

S

S

E

L

I

G

G

A

I

E

D

T

E

I

L

H

V

V

F

V

L

D

D

L

I

D

T

G

A

A

S

L

V

G

E

L

K

E

R

K

K

N

T

T

M

-

L

G

E

I

L

L

V

K

E

E

K

I

V

T

A

E

E

R

Q

V

I

S

D

V

I

P

P

L

F

Q

T

I

V

G

G

G
|
G

I

I

R

H

S

D

R

F

E

E

Y

T

A

A

G

S

K

E

L

L

L

I

D

L

M

R

G

G

F

A

E

D

R

K

V

V

I

S

L

I

G
x
N

T
|
T

M

A

A

A

I

V

E

E

N

N

P

P

A

S

I

L

V

I

E

T

E

Q

L

I

A

A

G

Q

E

T

Y

F

G

G

S

S

E

Q

R

A

I

V

M

V

V

V

S

A

L

I

D
|
D

S

A

-

K

-

R

-

V

-

D

-

G

-

E

-

F

-

M

-

V

-

F

-

T

-

Y

-

S

-

G

-

K

R

K

D

N

S

T

R

G

V

I

V

L

I

L

R

R

G

D

W

W

T

V

E

V

K

E

V

V

P

-

F

-

T

-

A

-

E

-

M

-

A

-

R

-

K

-

F

-

Q

E

E

K

R

R

G

G

A

A

G

G

S

E

I

I

L

L

F

L

T

T

N

S

V

I

D

D

F

R

E
x
D

G
|
G

L

T

L

K

S

S

G

G

F

Y

D

D

L

T

K

E

P

M

V

I

S

R

E

F

L

V

V

R

E

P

A

L

V

T

E

T

I

L

P

P

V

I

I

I

Y

A

S

S

G

G

V

A

S

G

S

K

L

M

N

E

D

H

L

F

active site: N11 (≠ D15), D130 (= D136)
binding phosphate ion: G82 (= G88), N103 (≠ G109), T104 (= T110), D176 (≠ E178), G177 (= G179)

Sites not aligning to the query:

binding phosphate ion: 225

2wjzE Crystal structure of (hish) k181a y138a mutant of imidazoleglycerolphosphate synthase (hish hisf) which displays constitutive glutaminase activity (see paper)
25% identity, 83% coverage: 10:212/246 of query aligns to 6:196/237 of 2wjzE

query
sites
2wjzE

I

I

P

A

A

C

V

L

D
|
D

I

V

K

K

D

D

G

G

K

R

C

-

V

-

Q

-

L

-

V

-

Q

-

G

-

K

-

P

-

G

-

T

-

E

-

Q

-

V

-

V

V

L

V

D

G

D

D

P

P

A

V

G

E

V

L

A

G

G

K

R

F

W

Y

E

S

S

E

L

I

G

G

A

I

E

D

T

-

I

-

H

E

V

L

V

V

D

F

L

L

D

D

G

I

A

T

L

A

G

K

L

R

E

K

K

T

N

M

T

L

G

E

I

L

L

V

K

E

E

K

I

V

T

A

E

E

R

Q

V

I

S

D

V

I

P

P

L

F

Q

T

I

V

G

G

G
|
G

I

I

R

H

S

D

R

F

E

E

Y

T

A

A

G

S

K

E

L

L

L

I

D

L

M

R

G

G

F

A

E

D

R

K

V

V

I

S

L

I

G
x
N

T
|
T

M

A

A

A

I

V

E

E

N

N

P

P

A

S

I

L

V

I

E

T

E

Q

L

I

A

A

G

Q

E

T

Y

F

G

G

S

S

E

Q

R

A

I

V

M

V

V

V

S

A

L

I

D
|
D

S

A

-

K

-

R

-

V

-

D

-

G

-

E

-

F

-

M

-

V

-

F

-

T

-

Y

-

S

-

G

-

K

R

K

D

N

S

T

R

G

V

I

V

L

I

L

R

R

G

D

W

W

T

V

E

V

K

E

V

V

P

-

F

-

T

-

A

-

E

-

M

-

A

-

R

-

K

-

F

-

Q

E

E

K

R

R

G

G

A

A

G

G

S

E

I

I

L

L

F

L

T

T

N

S

V

I

D

D

F

R

E

D

G

G

L

T

L

K

S

S

G

G

F

Y

D

D

L

T

K

E

P

M

V

I

S

R

E

F

L

V

V

R

E

P

A

L

V

T

E

T

I

L

P

P

V

I

I

I

Y

A

S

S

G

G

V

A

S

G

S

K

L

M

N

E

D

H

L

F

active site: D11 (= D15), D116 (= D136)
binding phosphate ion: G68 (= G88), N89 (≠ G109), T90 (= T110)

4ewnD Structure of hisf-d130v+d176v with bound rcdrp (see paper)
25% identity, 83% coverage: 10:212/246 of query aligns to 5:203/243 of 4ewnD

query
sites
4ewnD

I

I

P

A

A

C

V

L

D

D

I

V

K

K

D

D

G

G

K

R

C

V

V

V

Q

K

L

-

V

-

Q

-

G

-

K

-

P

-

G

G

T

T

E

N

Q

F

V

E

V

N

L

L

D

R

D

D

-

S

-

G

-

D

P

P

A

V

G

E

V

L

A

G

G

K

R

F

W

Y

E

S

S

E

L

I

G

G

A

I

E

D

T

E

I

L

H

V

V

F

V

L

D

D

L

I

D

T

G

K

A

T

L

M

G

-

L

L

E

E

K

-

N

-

T

-

G

-

I

L

L

V

K

E

E

K

I

V

T

A

E

E

R

Q

V

I

S

D

V

I

P

P

L

F

Q

T

I

V

G

G

I

I

R

H

S

D

R

F

E

E

Y

T

A

A

G

S

K

E

L

L

L

I

D

L

M

R

G

G

F

A

E

D

R

K

V

V

I

S

L

I

G

N

T

T

M

A

A

A

I

V

E

E

N

N

P

P

A

S

I

L

V

I

E

T

E

Q

L

I

A

A

G

Q

E

T

Y

F

G

G

S

S

E

Q

R

A

I

V

M

V

V

V

S

A

L

I

-
x
V

-

A

-

K

-

R

-

V

-

D

-

G

-

E

-

F

-

M

-

V

-

F

-

T

-

Y

-

S

D

G

S

K

R

K

D

N

S

T

R

G

V

I

V

L

I

L

R

R

G

D

W

W

T

V

E

V

K

E

V

V

P

-

F

-

T

-

A

-

E

-

M

-

A

-

R

-

K

-

F

-

Q

E

E

K

R

R

G

G

A

A

G

G

S

E

I

I

L

L

F

L

T

T

N

S

V

I

D

D

F

R

E

V

G
|
G

L

T

L

K

S

S

G

G

F

Y

D

D

L

T

K

E

P

M

V

I

S

R

E

F

L

V

V

R

E

P

A

L

V

T

E

T

I

L

P

P

V

I

I

I

Y

A

S
|
S

G
|
G

V

A

S

G

S

K

L

M

N

E

D

H

L

F

binding 1-(O-carboxy-phenylamino)-1-deoxy-D-ribulose-5-phosphate: V123 (vs. gap), G170 (= G179), S194 (= S203), G195 (= G204), G196 (= G205)

Sites not aligning to the query:

binding 1-(O-carboxy-phenylamino)-1-deoxy-D-ribulose-5-phosphate: 215, 216, 217, 218

5d2tA Directed evolutionary changes in kemp eliminase ke07 - crystal 3 wild type
22% identity, 95% coverage: 10:242/246 of query aligns to 4:238/251 of 5d2tA

query
sites
5d2tA

I

I

P

A

A

A

V

L

D

I

I

V

K

K

D

D

G

G

K

R

C

-

V

-

Q

-

L

V

V

V

Q

K

G

G

K

S

P

N

G

F

T

E

E

N

Q

L

V

R

V

D

L

S

D

G

D

D

P

P

A

V

G

E

V

L

A

G

G

K

R

F

W

Y

E

S

S

E

L

I

G

G

A

I

E

D

T

E

I

L

H

S

V

F

V
x
W

D

D

L

I

D

T

G

A

A

S

L

V

G

E

L

K

E

R

K

K

N

T

T

M

-

L

G

E

I

L

L

V

K

E

E

K

I

V

T

A

E

E

R

Q

V

I

S

D

V

I

P

P

L

F

Q

T

I

V

G

G

I

I

R

H

S

D

R

F

E

E

Y

T

A

A

G

S

K

E

L

L

L

I

D

L

M

R

G

G

F

A

E

D

R

K

V

V

I
x
E

L

I

G

N

T

T

M

A

A

A

I

V

E

E

N

N

P

P

A

S

I

L

V

I

E

T

E

Q

L

I

A

A

G

Q

E

T

Y

F

G

G

S

S

E

Q

R

A

I

V

M

V

V

V

S
x
Y

L

I

D

A

S

A

-

K

-

R

-

V

-

D

-

G

-

E

-

F

-

M

-

V

-

F

-

T

-

Y

-

S

-

G

-

K

R

K

D

N

S

T

R

G

V

I

V

L

I

L

R

R

G

D

W

W

T

V

E

V

K

E

V

V

P

-

F

-

T

-

A

-

E

-

M

-

A

-

R

-

K

-

F

-

Q

E

E

K

R

R

G

G

A

A

G

G

S

E

I

I

L

V

F

L

T

G

N

S

V

I

D

D

F

R

E

L

G

G

L

T

L

K

S

S

G

G

F

Y

D

D

L

T

K

E

P

M

V

I

S

R

E

F

L

V

V

R