SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_048061040.1 NCBI__GCF_000008645.1:WP_048061040.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q72IW9 Isocitrate/homoisocitrate dehydrogenase; Homoisocitrate dehydrogenase; HICDH; EC 1.1.1.286 from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see 4 papers)
51% identity, 94% coverage: 2:309/326 of query aligns to 4:316/334 of Q72IW9

query
sites
Q72IW9

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E57 (= E55) mutation to V: Confers enzyme activity with 3-isopropylmalate; when associated with I-72; M-85; A-86; T-208; Y-217; M-238 and M-310.
ATS 70:72 (≠ AGE 68:70) binding NADH
S72 (≠ E70) binding in other chain; mutation to I: Confers enzyme activity with 3-isopropylmalate; when associated with V-57; M-85; A-86; T-208; Y-217; M-238 and M-310.
R85 (≠ V77) binding in other chain; mutation to M: Confers enzyme activity with 3-isopropylmalate; when associated with V-57; I-72; A-86; T-208; Y-217; M-238 and M-310.; mutation to V: Confers low enzyme activity with 3-isopropylmalate. Reduces activity with homoisocitrate. Abolishes activity with isocitrate.
Y86 (≠ R78) mutation to A: Confers enzyme activity with 3-isopropylmalate; when associated with V-57; I-72; M-85; T-208; Y-217; M-238 and M-310.
R88 (= R80) binding in other chain
R98 (= R90) binding in other chain
R118 (= R111) binding in other chain
Y125 (= Y118) binding in other chain; mutation to A: Reduces catalytic efficiency with isocitrate.
V135 (≠ G128) mutation to M: Formation of homodimers instead of homotetramers. Increased affinity for isocitrate. Reduces enzyme activity with isocitrate.
K171 (= K164) binding (2R,3S)-homoisocitrate
N173 (= N166) binding (2R,3S)-homoisocitrate; binding NADH
D204 (= D197) binding Mg(2+)
M208 (= M201) mutation to T: Confers enzyme activity with 3-isopropylmalate; when associated with V-57; I-72; M-85; A-86; T-208; Y-217; M-238 and M-310.
F217 (= F210) mutation to Y: Confers enzyme activity with 3-isopropylmalate; when associated with V-57; I-72; M-85; A-86; T-208; M-238 and M-310.
D228 (= D221) binding Mg(2+)
D232 (= D225) binding Mg(2+)
V238 (≠ I231) mutation to M: Confers enzyme activity with 3-isopropylmalate; when associated with V-57; I-72; M-85; A-86; T-208; Y-217; and M-310.
GSAPD 261:265 (≠ GSAPQ 254:258) binding NADH
N273 (= N266) binding NADH
R310 (≠ V303) mutation to M: Confers enzyme activity with 3-isopropylmalate; when associated with V-57; I-72; M-85; A-86; T-208; Y-217; and M-238.

4yb4A Crystal structure of homoisocitrate dehydrogenase from thermus thermophilus in complex with homoisocitrate, magnesium ion (ii) and nadh
51% identity, 94% coverage: 2:309/326 of query aligns to 3:315/333 of 4yb4A

query
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4yb4A

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active site: Y124 (= Y118), K170 (= K164), D203 (= D197), D227 (= D221), D231 (= D225)
binding (1R,2S)-1-hydroxybutane-1,2,4-tricarboxylic acid: S71 (≠ E70), R84 (≠ V77), R87 (= R80), R97 (= R90), R117 (= R111), Y124 (= Y118), D227 (= D221)
binding 1,4-dihydronicotinamide adenine dinucleotide: I12 (= I11), A69 (= A68), T70 (≠ G69), S71 (≠ E70), I201 (≠ Y195), N204 (≠ A198), L240 (= L234), E256 (= E250), H259 (= H253), G260 (= G254), S261 (= S255), A262 (= A256), D264 (≠ Q258), I265 (= I259), N272 (= N266), D312 (= D306)

3asjB Crystal structure of homoisocitrate dehydrogenase in complex with a designed inhibitor (see paper)
51% identity, 94% coverage: 2:309/326 of query aligns to 3:315/333 of 3asjB

query
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active site: Y124 (= Y118), K170 (= K164), D203 (= D197), D227 (= D221), D231 (= D225)
binding (2Z)-3-[(carboxymethyl)sulfanyl]-2-hydroxyprop-2-enoic acid: R84 (≠ V77), R97 (= R90), R117 (= R111), Y124 (= Y118), D227 (= D221), D231 (= D225), V258 (= V252)

3asjA Crystal structure of homoisocitrate dehydrogenase in complex with a designed inhibitor (see paper)
51% identity, 94% coverage: 2:309/326 of query aligns to 3:315/333 of 3asjA

query
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3asjA

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K

A

A

N

N

V

V

L

L

K

P

K

L

T

T

D

Q

G

G

I

L

F

F

R

L

D

D

E

T

F

V

Y

K

K

E

V

V

A

A

S

K

E

D

Y

F

P

P

Q

L

M

V

E

N

A

V

T

Q

D

D

Y

I

V

V

D
|
D

A

N

T

C

A

A

M

M

Y

Q

L

L

I

V

T

M

Q

R

P

P

Q

E

E

R

F

F

Q

D

T

V

I

I

V

V

T

T

N

N

L

L

F

L

G

G

D
|
D

I

I

L

L

S

S

D
|
D

E

L

A

A

G

G

L

L

I

V

G

G

L

L

G

G

L

L

A

A

P

P

S

S

A

G

N

N

I

I

G

G

E

D

K

T

N

T

A

A

L

V

F

F

E

E

P

P

V

V

H

H

G

G

S

S

A

A

P

P

Q

D

I

I

A

A

G

G

K

K

N

G

I

I

A

A

N

N

P

P

T

T

A

A

M

A

I

I

L

L

T

S

T

A

L

M

M

M

L

L

K

D

H

Y

L

L

N

G

K

E

K

K

Q

E

E

A

A

A

Q

K

K

R

I

V

E

E

K

K

A

A

L

V

Q

D

K

L

T

V

L

L

A

E

E

R

G

G

L

P

V

R

T

T

P

P

D

D

L

L

G

G

active site: Y124 (= Y118), K170 (= K164), D203 (= D197), D227 (= D221), D231 (= D225)
binding 3-[(carboxymethyl)sulfanyl]-2-oxopropanoic acid: R97 (= R90), R117 (= R111), Y124 (= Y118), D227 (= D221), D231 (= D225)

6m3sB Dimeric isocitrate dehydrogenase from xanthomonas campestris pv. Campestris 8004
46% identity, 99% coverage: 3:324/326 of query aligns to 8:338/338 of 6m3sB

query
sites
6m3sB

I

I

A

T

V

V

I

I

P

R

G

G

D

D

G

G

I

I

G

G

V

P

E

E

V

I

M

M

E

D

A

A

A

T

L

L

H

F

I

V

L

L

N

D

T

A

L

L

D

Q

L

A

D

G

L

L

E

T

F

Y

I

E

H

Y

A

A

D

D

A

A

G

G

D

L

A

V

C

A

L

L

K

E

R

K

T

H

G

G

T

D

A

L

L

L

P

P

E

E

E

S

T

T

L

L

E

A

A

S

V

I

G

T

E

K

A

N

R

K

A

V

T

A

L

L

F

K

G

S

A
x
P

-
x
L

-

T

-
x
T

-

P

A

V

G

G

E

E

S

G

A

F

A

S

D
x
S

V

I

I
x
N

V

V

R

A

L

M

R
|
R

R

R

E

K

F

F

D

D

L

L

F

Y

A

A

N

N

L

V

R
|
R

P

P

V

A

K

K

S

S

L

F

P

P

G

N

V

T

P

K

C

S

L

R

Y

F

P

A

D

D

-

G

L

V

D

D

F

L

V

I

I

T

V

V

R
|
R

E

E

N

N

T

T

E

E

D

G

L

A

Y
|
Y

V

L

G

S

D

E

-

G

E

Q

E

E

Y

V

T

S

P

A

E

D

G

G

-

E

-

V

A

A

V

V

A

S

K

G

R

A

I

R

I

V

T

T

R

R

T

K

A

G

S

S

R

E

R

R

I

I

S

V

Q

R

F

Y

A

A

F

F

Q

D

Y

L

A

A

Q

R

K

A

E

T

G

G

M

R

Q

K

K

K

V

V

T

T

A

A

V

V

H

H

K
|
K

A

A

N

N

V

I

L

I

K

K

K

S

T

T

D

S

G

G

I

L

F

F

R

L

D

K

E

V

F

A

Y

R

K

D

V

V

A

A

S

T

E

Q

Y

Y

P

P

Q

E

M

I

E

E

A

F

T

Q

D

E

Y

M

Y

I

V

V

D
|
D

A

N

T

T

A

C

M

M

Y

Q

L

L

I

V

T

M

Q

R

P

P

Q

E

E

Q

F

F

Q

D

T

I

I

I

V

V

T

T

N

N

L

L

F

F

G

G

D
|
D

I

I

L

I

S

S

D
|
D

E

L

A

C

A

A

G

G

L

L

I

V

G

G

L

L

G

G

L

L

A

A

P

P

S

G

A

A

N

N

I

I

G

G

E

V

K

D

N

A

A

A

L

I

F

F

E

E

P

A

V

V

H
|
H

G
|
G

S
|
S

A
|
A

P

P

Q
x
D

I
|
I

A

A

G

G

K

Q

N

G

I

K

A

A

N
|
N

P

P

T

C

A

A

M

L

I

L

L

L

T

G

T

A

L

Q

M

M

L

L

K

D

H

H

L

I

N

G

K

Q

K

P

Q

Q

E

N

A

A

Q

E

K

R

I

L

E

R

K

E

A

A

L

I

Q

V

K

A

T

T

L

L

-

E

A

A

E

K

G

D

L

S

V

L

T

T

P

P

D

D

L

L

G

G

K

T

L

G

G

N

T

T

M

M

E

G

M

F

A

A

A

K

E

A

I

I

A

A

R

S

H

R

L

L

active site: Y128 (= Y118), K177 (= K164), D210 (= D197), D234 (= D221)
binding isocitrate calcium complex: T75 (vs. gap), S83 (≠ D74), N85 (≠ I76), R89 (= R80), R99 (= R90), R121 (= R111), Y128 (= Y118), D234 (= D221), D238 (= D225)
binding nicotinamide-adenine-dinucleotide: P72 (≠ A67), L73 (vs. gap), T75 (vs. gap), N85 (≠ I76), H266 (= H253), G267 (= G254), S268 (= S255), A269 (= A256), D271 (≠ Q258), I272 (= I259), N279 (= N266)

6lkyA Crystal structure of isocitrate dehydrogenase from methylococcus capsulatus
44% identity, 99% coverage: 2:325/326 of query aligns to 3:338/339 of 6lkyA

query
sites
6lkyA

K

K

I

I

A

T

V

L

I

I

P

P

G

G

D

D

G

G

I

I

G

G

V

P

E

S

V

I

M

V

E

D

A

A

L

V

H

K

I

V

L

I

N

E

T

A

L

T

D

G

L

V

D

Q

L

V

E

Q

F

W

I

D

H

T

A

Q

D

S

A

A

G

G

D

M

A

A

C

A

L

V

K

E

R

K

T

F

G

G

T

T

A

P

L

L

P

P

E

D

E

A

T

T

L

L

E

D

A

S

V

I

G

R

E

A

A

N

R

R

A

I

T

C

L

F

F

K

G

G

A
x
P

-
x
L

-

T

-
x
T

-

P

A

V

G

G

E

G

S

G

A

Y

A

R

D

S

V

V

I
x
N

V

V

R

T

L

L

R

R

R

Q

E

A

F

L

D

N

L

L

F

Y

A

A

N

N

L

V

R

R

P

P

V

A

K

I

S

S

L

F

P

E

G

G

V

T

P

D

C

T

L

A

Y

F

P

S

D

D

L

V

D

N

F

L

V

V

I

T

V

V

R

R

E

E

N

N

T

T

E

E

D

G

L

L

Y
|
Y

V

A

G

G

-

I

-

E

-

H

-

F

-

I

-

K

-

V

D

D

E

E

Y

K

T

I

P

A

E

A

G

E

A

S

V

I

A

A

K

-

R

-

I

V

T

T

R

R

T

K

A

G

S

S

R

E

R

R

I

I

S

I

Q

R

F

Y

A

A

F

F

Q

D

Y

Y

A

A

Q

R

K

R

E

A

G

R

M

R

Q

K

K

K

V

V

T

T

A

L

V

V

H

H

K
|
K

A

A

N

N

V

I

L

L

K

K

K

C

T

T

D

S

G

G

I

L

F

F

R

L

D

E

E

I

F

G

Y

R

K

E

V

I

A

A

S

K

E

E

Y

Y

P

P

Q

D

M

I

E

E

A

F

T

D

D

D

Y

R

Y

I

V

V

D
|
D

A

A

T

C

A

S

M

M

Y

Q

L

M

I

V

T

M

Q

Q

P

P

Q

Q

E

R

F

F

Q

D

T

V

I

L

V

V

T

T

N

N

L

L

F

F

G

G

D
|
D

I

I

L

L

S

S

D

D

E

L

A

A

G

G

L

L

I

I

G

G

L

L

G

G

L

L

A

T

P

A

S

G

A

A

N

N

I

I

G

G

E

T

K

D

N

A

A

A

L

L

F

F

E

E

P

A

V

V

H
|
H

G
|
G

S
|
S

A
|
A

P

P

Q
x
D

I
|
I

A

A

G

D

K

K

N

G

I

I

A

A

N
|
N

P

P

T

T

A

A

M

M

I

I

L

M

T

A

T

G

T

A

L

M

M

M

L

L

K

E

H

H

L

I

N

G

K

E

K

P

Q

D

E

A

A

A

Q

R

K

R

I

I

E

E

K

R

A

A

L

V

Q

R

K

E

T

V

L

I

A

E

E

D

G

G

L

R

-

S

V

V

T

T

P

P

D

D

L

L

G

A

-

K

-

D

G

S

K

P

L

C

G

G

T

T

M

A

E

Q

M

M

A

A

A

E

E

A

I

I

A

V

R

E

H

R

L

V

E

R

active site: Y123 (= Y118), K174 (= K164), D207 (= D197), D231 (= D221)
binding nicotinamide-adenine-dinucleotide: P68 (≠ A67), L69 (vs. gap), T71 (vs. gap), N81 (≠ I76), H263 (= H253), G264 (= G254), S265 (= S255), A266 (= A256), D268 (≠ Q258), I269 (= I259), N276 (= N266)

4y1pB Crystal structure of 3-isopropylmalate dehydrogenase (saci_0600) from sulfolobus acidocaldarius complex with 3-isopropylmalate and mg2+ (see paper)
45% identity, 98% coverage: 3:320/326 of query aligns to 4:331/336 of 4y1pB

query
sites
4y1pB

I

V

A

A

V

L

I

I

P

Q

G

G

D

D

G

G

I

I

G

G

V

P

E

E

V

V

M

V

E

S

A

K

A

S

L

K

H

T

I

I

L

L

N

A

T

R

L

L

D

N

-

E

-

K

-

F

-

S

L

L

D

P

L

I

E

E

F

Y

I

I

H

E

A

V

D

E

A

A

G

G

D

D

A

T

C

T

L

K

K

N

R

K

T

F

G

G

T

D

A

A

L

L

P

P

E

K

E

D

T

S

L

L

E

R

A

V

V

I

G

E

E

K

A

A

R

D

A

M

T

I

L

L

F

K

G

G

A

P

A

V

G

G

E

E

S

T

A

A

A
|
A

D

D

V

V

I

V

V

V

R

K

L

L

R
|
R

R

L

E

M

F

Y

D

D

L

L

F

Y

A

A

N

N

L

L

R
|
R

P

P

V

A

K

K

S

S

L

L

P

P

G

G

V

L

P

E

C

N

L

K

Y

F

P

G

D

D

L

V

D

D

F

I

V

L

I

V

V

V

R
|
R

E

E

N

N

T

T

E

E

D

D

L

L

Y
|
Y

V

K

G

G

D

L

E

E

E

H

Y

V

T

I

P

S

E

D

G

G

A

V

-

T

V

V

A

G

K

I

R

K

I

V

I

I

T

T

R

R

T

A

A

A

S

S

R

T

R

R

I

I

S

A

Q

Q

F

V

A

A

F

L

Q

N

Y

Q

A

A

Q

L

K

R

E

R

G

-

M

K

Q

K

K

K

V

V

T

V

A

C

V

V

H

H

K
|
K

A

S

N

N

V

V

L

M

K

R

K

I

T

T

D

D

G

G

I

L

F

F

R

A

D

E

E

S

F

C

Y

R

K

N

V

V

A

L

S

K

E

-

Y

-

P

G

Q

K

M

V

E

E

A

Y

T

S

D

E

Y

M

Y

Y

V

V

D
|
D

A

A

T

A

A

A

M

A

Y

N

L

L

I

V

T

R

Q

N

P

P

Q

Q

E

A

F

F

Q

D

T

V

I

I

V

V

T

T

T

E

N

N

L

T

F

Y

G

G

D
|
D

I

I

L

L

S

S

D
|
D

E

E

A

A

A

G

G

Q

L

I

I

A

G

G

G

S

L

L

G

G

L

I

A

S

P

P

S

S

A

A

N

N

I

I

G

G

E

D

K

R

N

K

A

S

L

L

F

F

E

E

P

P

V

V

H

H

G

G

S

A

A

A

P

F

Q

D

I

I

A

A

G

G

K

K

N

N

I

I

A

A

N

N

P

P

T

T

A

A

M

F

I

L

L

L

T

S

T

V

T

G

L

M

M

M

L

L

K

D

H

R

L

M

N

Q

K

E

-

L

-

S

-

G

-

D

-

I

-

R

-

Y

K

N

Q

N

E

A

A

A

Q

K

K

S

I

L

E

R

K

D

A

A

L

I

Q

Y

K

S

T

V

L

Y

A

S

E

E

G

G

-

K

L

Y

V

L

T

T

P

P

D

D

L

V

G

G

K

S

L

S

G

T

T

T

M

D

E

E

M

M

A

I

A

S

E

A

I

I

active site: Y123 (= Y118), K169 (= K164), D200 (= D197), D224 (= D221), D228 (= D225)
binding 3-isopropylmalic acid: A78 (= A73), R85 (= R80), R95 (= R90), R116 (= R111), Y123 (= Y118), D224 (= D221)

2d1cA Crystal structure of tt0538 protein from thermus thermophilus hb8
45% identity, 92% coverage: 3:303/326 of query aligns to 21:329/495 of 2d1cA

query
sites
2d1cA

I

I

A

T

V

V

I

I

P

P

G

G

D

D

G

G

I

I

G

G

V

P

E

E

V

C

M

V

E

E

A

A

A

T

L

L

H

K

I

V

L

L

N

E

T

A

L

A

D

K

L

A

D

P

L

L

E

A

F

Y

I

E

H

V

A

R

D

E

A

A

G

G

D

A

A

S

C

V

L

F

K

R

R

R

-

G

T

I

G

A

T

S

A

G

L

V

P

P

E

Q

E

E

T

T

L

I

E

E

A

S

V

I

G

R

E

K

A

T

R

R

A

V

T

V

L

L

F

K

G

G

-
x
P

-
x
L

-
x
E

-
x
T

-

P

-

V

A

G

A

Y

G

G

E

E

S

K

A

S

A

A

D
x
N

V

V

I

T

V

-

R

-

L

L

R

R

R

K

E

L

F

F

D

E

L

T

F

Y

A

A

N

N

L

V

R

R

P

P

V

V

K

R

S

E

L

F

P

P

G

N

V

V

P

P

C

T

L

P

Y

Y

P

A

D

G

-

R

-

G

L

I

D

D

F

L

V

V

I

V

V

V

R

R

E

E

N

N

T

V

E

E

D

D

L

L

Y
|
Y

V

A

G

G

D

I

E

E

E

H

Y

M

-

Q

T

T

P

P

E

S

G

V

A

A

V

Q

A

T

K

L

R

K

I

L

I

I

T

S

R

W

T

K

A

G

S

S

R

E

R

K

I

I

S

V

Q

R

F

F

A

A

F

F

Q

E

Y

L

A

A

Q

R

K

A

E

E

G

G

M

R

Q

K

K

K

V

V

T

H

A

C

V

A

H

T

K
|
K

A

S

N

N

V

I

L

M

K

K

K

L

T

A

D

E

G

G

I

T

F

L

R

K

D

R

E

A

F

F

Y

E

K

Q

V

V

A

A

S

Q

E

E

Y

Y

P

P

Q

D

M

I

E

E

A

A

T

V

D

H

Y

I

Y
x
I

V

V

D
|
D

A
x
N

T

A

A

A

M

H

Y
x
Q

L

L

I

V

T

K

Q

R

P

P

Q

E

E

Q

F

F

Q

E

T

V

I

I

V

V

T

T

N

N

L

M

F

N

G

G

D
|
D

I

I

L

L

S

S

D
|
D

E

L

A

T

A

S

G

G

L

L

I

I

G

G

L
|
L

G
|
G

L

F

A

A

P

P

S

S

A

A

N

N

I

I

G

G

E

N

K

E

N

V

A

A

L

I

F

F

E

E

P

A

V

V

H
|
H

G
|
G

S
|
S

A
|
A

P

P

Q
x
K

I
x
Y

A

A

G

G

K

K

N

N

I

V

A
x
I

N
|
N

P

P

T

T

A

A

M

V

I

L

L

L

T

S

T

A

T

V

L

M

M

M

L

L

K

R

H

Y

L

L

N

E

K

E

K

F

Q

A

E

T

A

A

Q

D

K

L

I

I

E

E

K

N

A

A

L

L

Q

L

K

Y

T

T

L

L

A

E

E

E

G

G

L

R

V

V

active site: Y143 (= Y118), K190 (= K164), D223 (= D197), D247 (= D221), D251 (= D225)
binding nadp nicotinamide-adenine-dinucleotide phosphate: P86 (vs. gap), L87 (vs. gap), E88 (vs. gap), T89 (vs. gap), N99 (≠ D74), I221 (≠ Y195), N224 (≠ A198), Q228 (≠ Y202), L260 (= L234), G261 (= G235), H279 (= H253), G280 (= G254), S281 (= S255), A282 (= A256), K284 (≠ Q258), Y285 (≠ I259), I291 (≠ A265), N292 (= N266)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 333

P40495 Homoisocitrate dehydrogenase, mitochondrial; HIcDH; EC 1.1.1.87 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
43% identity, 99% coverage: 1:324/326 of query aligns to 24:371/371 of P40495

query
sites
P40495

M

L

K

T

I

I

A

G

V

L

I

I

P

P

G

G

D

D

G

G

I

I

G

G

V

K

E

E

V

V

M

I

E

P

A

A

A

G

L

K

H

Q

I

V

L

L

N

E

T

N

L

L

D

N

-

S

-

K

-

H

-

G

L

L

D

S

L

F

E

N

F

F

I

I

H

D

A

L

D

Y

A

A

G

G

D

F

A

Q

C

T

L

F

K

Q

R

E

T

T

G

G

T

K

A

A

L

L

P

P

E

D

E

E

T

T

L

V

E

K

A

V

V

L

G

K

E

E

-

Q

A

C

R

Q

A

G

T

A

L

L

F

F

G

G

A

A

A

V

G

Q

E

S

S

P

A

T

D

K

V

V

-

E

-

G

-

Y

-

S

-

P

I

I

V

V

R

A

L

L

R

R

E

E

F

M

D

G

L

L

F

F

A

A

N

N

L

V

R

R

P

P

V

V

K

K

S

S

L

V

P

E

G

G

V

E

P

K

C

G

L

-

Y

-

P

K

D

P

L

I

D

D

F

M

V

V

I

I

V

V

R

R

E

E

N

N

T

T

E

E

D

D

L

L

Y
|
Y

V

I

G

K

D

I

E

E

E

K

Y

T

T

Y

P

I

E

D

G

K

A

A

V

T

A

G

K

T

R

R

I

V

-

A

-

D

-

A

-

T

-

K

-

R

I

I

T

S

R

E

T

I

A

A

S

T

R

R

I

I

S

A

Q

T

F

I

A

A

F

L

Q

D

Y

I

A

A

-

L

-

K

-

R

-

L

Q

Q

K

T

E

R

G

G

M

Q

Q

A

K

T

V

L

T

T

A

V

V

T

H

H

K
|
K

A

S

N

N

V

V

L

L

K

S

K

Q

T

S

D

D

G

G

I

L

F

F

R

R

D

E

-

I

-

C

-

K

E

E

F

V

Y

Y

K

E

V

S

A

N

S

K

E

D

-

K

Y

Y

P

G

Q

Q

M

I

E

K

A

Y

T

N

D

E

Y

Q

Y

I

V

V

D

D

A

S

T

M

A

V

M

Y

Y

R

L

L

I

F

T

R

Q

E

P

P

Q

Q

E

C

F

F

Q

D

T

V

I

I

V

V

T

A

T

P

N

N

L

L

F

Y

G

G

D

D

I

I

L

L

S

S

D

D

E

G

A

A

G

A

L

L

I

V

G

G

G

S

L

L

G

G

L

V

A

V

P

P

S

S

A

A

N

N

I

V

G

G

E

P

K

E

N

I

A

V

L

I

F

G

E

E

P

P

V

C

H

H

G

G

S

S

A

A

P

P

Q

D

I

I

A

A

G

G

K

K

N

G

I

I

A

A

N

N

P

P

T

I

A

A

M

T

I

I

L

R

T

S

T

T

T

A

L

L

M

M

L

L

K

E

H

F

L

L

N

G

K

H

K

N

Q

E

E

A

A

A

Q

Q

K

D

I

I

E

Y

K

K

A

A

L

V

Q

D

K

A

T

N

L

L

A

R

E

E

G

G

L

S

V

I

-

K

T

T

P

P

D

D

L

L

G

G

K

K

L

A

G

S

T

T

M

Q

E

Q

M

V

A

V

A

D

E

D

I

V

A

L

R

S

H

R

L

L

Y150 (= Y118) mutation to F: Strongly reduced enzyme activity.
K206 (= K164) mutation to M: Strongly reduced enzyme activity.

8grdA Crystal structure of a constitutively active mutant of the alpha beta heterodimer of human idh3 in complex with adp and mg (see paper)
39% identity, 99% coverage: 3:326/326 of query aligns to 4:325/325 of 8grdA

query
sites
8grdA

I

V

A

T

V

L

I

I

P

P

G

G

D

D

G

G

I

I

G

G

V

P

E

E

V

I

M

S

E

A

A

A

A

V

L

M

H

K

I

I

L

F

N

D

T

A

L

A

D

K

L

A

D

P

L

I

E

Q

F

W

I

E

H

E

A

R

D

N

A

V

G

-

D

-

A

-

C

-

L

T

K

A

R

I

T

Q

G

G

T

W

A

M

L

I

P

P

E

S

E

E

T

A

L

K

E

E

A

S

V

M

G

D

E

K

A

N

R

K

A

M

T

G

L

L

F

K

G

G

A

P

A

L

G

-

E

K

S

T

A

P

D

S

V

M

I

N

V

L

R

L

L

L

R

R

R

K

E

T

F

F

D

D

L

L

F

Y

A

A

N

N

L

V

R

R

P

P

V

C

K

V

S

S

L

I

P

E

G

G

V

Y

P

K

C

T

L

P

Y

Y

P

T

D

D

L

V

D

N

F

I

V

V

I

T

V

I

R

R

E

E

N

N

T

T

E

E

D

G

L

E

Y

Y

V

S

G

G

D

I

E

E

E

H

Y

V

T

I

P

V

E

D

G

G

A

V

V

V

A

A

K

S

-

I

R

K

I

L

I

I

T

T

R

E

T

G

A

A

S

S

R

K

R

R

I

I

S

A

Q

E

F

F

A

A

F

F

Q

E

Y

Y

A

A

Q

R

K

N

E

N

G

H

M

R

Q

S

K

N

V

V

T

T

A

A

V

V

H

H

K

K

A

A

N

N

V

I

L

M

K

R

K

M

T

S

D

D

G

G

I

L

F

F

R

L

D

Q

E

K

F

C

Y

R

K

E

V

V

A

A

S

E

E

S

Y

C

P

K

Q

D

M

I

E

K

A

F

T

N

D

E

Y

M

Y
|
Y

V

L

D

D

A
x
T

T

V

A

C

M

L

Y

N

L

M

I

V

T

Q

Q

D

P

P

Q

S

E

Q

F

F

Q

D

T

V

I

L

V

V

T

M

T

P

N

N

L

L

F

Y

G

G

D
|
D

I

I

L

L

S

S

D
|
D

E

L

A

C

A

A

G

G

L

L

I

I

G

G

L

L

G

G

L

V

A

T

P

P

S

S

A

G

N

N

I

I

G

G

E

A

K

N

N

G

-

V

A

A

L

I

F

F

E

E

P

S

V

V

H

H

G

G

S

T

A

A

P

P

Q

D

I

I

A

A

G

G

K

K

N

D

I

M

A

A

N

N

P

P

T

T

A

A

M

L

I

L

L

L

T

S

T

A

T

V

L

M

M

M

L

L

K

R

H

H

L

M

N

G

K

L

K

F

Q

D

E

H

A

A

Q

A

K

R

I

I

E

E

K

A

A

A

L

C

Q

F

K

A

T

T

L

I

A

K

E

D

G

G

-

K

L

S

V

L

T

T

P

K

D

D

L

L

G

G

K

N

L

A

G

K

T

C

M

S

E

D

M

F

A

T

A

E

E

E

I

I

A

C

R

R

H

R

L

V

E

K

D

D

binding adenosine-5'-diphosphate: Y192 (= Y195), T195 (≠ A198)
binding magnesium ion: D218 (= D221), D222 (= D225)

6kdeA Crystal structure of the alpha beta heterodimer of human idh3 in complex with ca(2+) (see paper)
38% identity, 99% coverage: 3:326/326 of query aligns to 5:335/336 of 6kdeA

query
sites
6kdeA

I

V

A

T

V

L

I

I

P

P

G

G

D

D

G

G

I

I

G

G

V

P

E

E

V

I

M

S

E

A

A

A

A

V

L

M

H

K

I

I

L

F

N

D

T

A

L

A

D

K

L

A

D

P

L

I

E

Q

F

W

I

E

H

E

A

R

D

N

A

V

G

T

D

A

A

I

C

Q

L

G

K

P

R

G

T

G

G

K

T

W

A

M

L

I

P

P

E

S

E

E

T

A

L

K

E

E

A

S

V

M

G

D

E

K

A

N

R

K

A

M

T

G

L

L

F

K

G

G

-

P

-

L

-

K

-

T

-

P

-

I

A

A

G

G

E

H

S

P

A

S

A

M

D

N

V

L

I

L

V

-

R

-

L

L

R

R

R

K

E

T

F

F

D

D

L

L

F

Y

A

A

N

N

L

V

R

R

P

P

V

C

K

V

S

S

L

I

P

E

G

G

V

Y

P

K

C

T

L

P

Y

Y

P

T

D

D

L

V

D

N

F

I

V

V

I

T

V

I

R

R

E

E

N

N

T

T

E

E

D

G

L

E

Y
|
Y

V

S

G

G

D

I

E

E

E

H

Y

V

T

I

P

V

E

D

G

G

A

V

V

V

A

Q

K

S

-

I

R

K

I

L

I

I

T

T

R

E

T

G

A

A

S

S

R

K

R

R

I

I

S

A

Q

E

F

F

A

A

F

F

Q

E

Y

Y

A

A

Q

R

K

N

E

N

G

H

M

R

Q

S

K

N

V

V

T

T

A

A

V

V

H

H

K
|
K

A

A

N

N

V

I

L

M

K

R

K

M

T

S

D

D

G

G

I

L

F

F

R

L

D

Q

E

K

F

C

Y

R

K

E

V

V

A

A

S

E

E

S

Y

C

P

K

Q

D

M

I

E

K

A

F

T

N

D

E

Y

M

Y

Y

V

L

D
|
D

A

T

T

V

A

C

M

L

Y

N

L

M

I

V

T

Q

Q

D

P

P

Q

S

E

Q

F

F

Q

D

T

V

I

L

V

V

T

M

T

P

N

N

L

L

F

Y

G

G

D
|
D

I

I

L

L

S

S

D
|
D

E

L

A

C

A

A

G

G

L

L

I

I

G

G

L

L

G

G

L

V

A

T

P

P

S

S

A

G

N

N

I

I

G

G

E

A

K

N

N

G

-

V

A

A

L

I

F

F

E

E

P

S

V

V

H

H

G

G

S

T

A

A

P

P

Q

D

I

I

A

A

G

G

K

K

N

D

I

M

A

A

N

N

P

P

T

T

A

A

M

L

I

L

L

L

T

S

T

A

T

V

L

M

M

M

L

L

K

R

H

H

L

M

N

G

K

L

K

F

Q

D

E

H

A

A

Q

A

K

R

I

I

E

E

K

A

A

A

L

C

Q

F

K

A

T

T

L

I

A

K

E

D

G

G

-

K

L

S

V

L

T

T

P

K

D

D

L

L

G

G

K

N

L

A

G

K

T

C

M

S

E

D

M

F

A

T

A

E

E

E

I

I

A

C

R

R

H

R

L

V

E

K

D

D

active site: Y124 (= Y118), K171 (= K164), D204 (= D197), D228 (= D221)
binding calcium ion: D228 (= D221), D232 (= D225)

6kdyA Crystal structure of the alpha bata heterodimer of human idh3 in complex with NAD. (see paper)
38% identity, 99% coverage: 3:326/326 of query aligns to 5:335/335 of 6kdyA

query
sites
6kdyA

I

V

A

T

V

L

I

I

P

P

G

G

D

D

G

G

I

I

G

G

V

P

E

E

V

I

M

S

E

A

A

A

A

V

L

M

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D

active site: Y124 (= Y118), K171 (= K164), D204 (= D197), D228 (= D221)
binding nicotinamide-adenine-dinucleotide: P69 (vs. gap), L70 (vs. gap), T72 (vs. gap), N82 (≠ D74), H261 (= H253), G262 (= G254), T263 (≠ S255), A264 (= A256), D266 (≠ Q258), I267 (= I259), N274 (= N266), D315 (= D306)

P50213 Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; Isocitric dehydrogenase subunit alpha; NAD(+)-specific ICDH subunit alpha; EC 1.1.1.41 from Homo sapiens (Human) (see 5 papers)
38% identity, 99% coverage: 3:326/326 of query aligns to 34:364/366 of P50213

query
sites
P50213

I

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R115 (= R80) binding substrate
A122 (= A87) to T: in RP90; uncertain significance; dbSNP:rs756333430
R125 (= R90) binding substrate
R146 (= R111) binding substrate
E152 (≠ L117) mutation to A: No significant effect on the activation of the heterodimer composed of IDH3A and IDH3G subunits by citrate and ADP.
Y153 (= Y118) Critical for catalysis; mutation to F: Complete loss of activity of the heterotetramer, heterodimer composed of IDH3A and IDH3B subunits and the heterodimer composed of IDH3A and IDH3G subunits with no effect on their oligomeric states.
K169 (≠ R133) mutation to A: Significantly impairs the activation of the heterodimer composed of IDH3A and IDH3G subunits by citrate and ADP.
A175 (= A139) to V: in RP90; uncertain significance; dbSNP:rs765473830
K200 (= K164) Critical for catalysis; mutation to A: Significantly impairs the activation of the heterodimer composed of IDH3A and IDH3G subunits by citrate.
N202 (= N166) mutation to A: Significantly impairs the activation of the heterodimer composed of IDH3A and IDH3G subunits by citrate.
M204 (≠ L168) to I: in RP90; uncertain significance
D208 (= D172) mutation to A: Complete loss of the activation of the heterodimer composed of IDH3A and IDH3G subunits by citrate and ADP.
D233 (= D197) binding Mg(2+)
M239 (≠ L203) to T: in RP90; uncertain significance; dbSNP:rs2074707744
Y255 (≠ F219) mutation to A: Significantly impairs the activation of the heterodimer composed of IDH3A and IDH3G subunits by citrate and ADP.
D257 (= D221) binding Mg(2+)
D261 (= D225) binding Mg(2+)
P304 (= P267) to H: in RP90; uncertain significance; dbSNP:rs756712426
M313 (≠ L276) to T: in RP90; uncertain significance; dbSNP:rs149862950
R316 (≠ K279) to C: in RP90; uncertain significance; dbSNP:rs770798851

6l59A Crystal structure of the alpha gamma heterodimer of human idh3 in complex with cit, mg and atp binding at allosteric site and mg, atp binding at active site. (see paper)
39% identity, 99% coverage: 3:326/326 of query aligns to 4:323/325 of 6l59A

query
sites
6l59A

I

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