SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_048061335.1 NCBI__GCF_000008645.1:WP_048061335.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1gdeA Crystal structure of pyrococcus protein a-1 e-form (see paper)
46% identity, 99% coverage: 4:370/372 of query aligns to 2:382/388 of 1gdeA

query
sites
1gdeA

F

L

A

S

S

D

R

R

V

L

K

E

D

L

I

V

Q

S

L

A

S

S

E

E

I

I

R

R

K

K

I

L

F

F

E

D

V

I

A

A

D

A

-

G

-

M

E

K

D

D

T

V

I

I

N

S

L

L

G

G

I

I

G

G

E

E

P

P

D

D

F

F

S

D

V

T

P

P

D

Q

H

H

V

I

R

K

E

E

A

Y

V

A

K

K

D

E

A

A

V

L

D

D

E

K

G

G

L

L

T

T

H

H

Y

Y

T

G

S

P

N

N

M

I

G

G

M

L

E

L

E

E

L

L

R

R

E

E

A

A

I

I

A

A

D

E

K

K

L

L

K

K

S

K

E

Q

N

N

R

G

V

I

H

E

A

A

E

D

P

P

E

K

S

T

-

E

I

I

I

M

V

V

T

L

V

L

G
|
G

A
|
A

S
x
N

E

Q

A

A

I

F

F

L

M

M

C

G

T

L

Q

S

A

A

L

F

L

L

D

K

I

D

G

G

D

E

H

E

A

V

L

L

I

I

P

P

D

T

P

P

G

A

F
|
F

L

V

S

S

Y

Y

D

A

A

P

C

A

V

V

R

I

L

L

S

A

G

G

A

G

V

K

S

P

I

V

P

E

V

V

P

P

L

T

S

Y

M

E

D

E

E

D

G

E

F

F

S

R

M

L

S

N

P

V

E

D

R

E

V

L

E

K

S

K

L

Y

I

V

T

T

Q

D

D

K

T

T

R

R

V

A

I

L

I

I

M

I

N
|
N

S

S

P

P

S

C

N
|
N

P

P

T

T

G

G

S

A

V

V

M

L

G

T

K

K

D

K

D

D

V

L

K

E

G

E

I

I

A

A

E

D

I

F

A

V

E

V

D

E

N

H

D

D

L

L

I

I

I

V

I

I

S

S

D
|
D

E

E

I

V

Y
|
Y

E

E

K

H

I

F

I

I

Y

Y

-

D

D

D

G

A

K

R

H

H

Y

Y

S

S

P

I

A

A

Q

S

F

L

T

D

-

G

-

M

-

F

D

E

N

R

A

T

L

I

I

T

V

V

N

N

G

G

F

F

S
|
S

K
|
K

T

T

Y

F

A

A

M

M

T

T

G

G

L

W

R
|
R

I

L

G

G

Y

F

V

V

A

A

G

A

C

P

E

S

D

W

I

I

I

I

E

E

E

R

L

M

L

V

K

K

V

F

H

Q

Q

M

Y

Y

N

N

T

A

A

T

C

C

A

P

P

V

S

T

I

F

S

I

Q

Q

Y

Y

A

A

A

A

L

A

A

K

A

A

I

L

R

K

G

D

P

E

Q

R

N

S

C

W

-

K

-

A

V

V

K

E

D

E

M

M

V

R

D

K

E

E

F

Y

R

D

R

R

R

R

R

R

D

K

L

L

M

V

F

W

R

K

S

R

L

L

T

N

D

E

M

M

G

G

L

L

E

P

C

T

V

V

L

K

P

P

G

K

G

G

A

A

F

F

Y

Y

M

I

F

F

P

P

Y

R

A

I

G

R

D

D

-

T

-

G

-

L

-

T

S

S

E

K

E

K

F

F

T

S

K

E

L

L

S

M

L

L

-

K

E

E

A

A

G

R

V

V

A

A

V

V

V

V

P

P

G

G

S

S

A

A

F

F

G

G

N

K

E

A

G

G

K

E

G

G

Y

Y

I

V

R
|
R

M

I

S

S

Y

Y

A

A

T

T

S

A

Y

Y

E

E

L

K

I

L

E

E

E

E

A

A

M

M

E

D

R

R

L

M

K

E

T

R

V

V

C

L

active site: K232 (= K227)
binding glutamic acid: F120 (= F119), N170 (= N169), R361 (= R349)
binding pyridoxal-5'-phosphate: G94 (= G93), A95 (= A94), N96 (≠ S95), F120 (= F119), N166 (= N165), D198 (= D197), Y201 (= Y200), S231 (= S226), K232 (= K227), R240 (= R235)

1gd9A Crystall structure of pyrococcus protein-a1 (see paper)
46% identity, 99% coverage: 4:370/372 of query aligns to 2:382/388 of 1gd9A

query
sites
1gd9A

F

L

A

S

S

D

R

R

V

L

K

E

D

L

I

V

Q

S

L

A

S

S

E

E

I

I

R

R

K

K

I

L

F

F

E

D

V

I

A

A

D

A

-

G

-

M

E

K

D

D

T

V

I

I

N

S

L

L

G

G

I

I

G

G

E

E

P

P

D

D

F

F

S

D

V

T

P

P

D

Q

H

H

V

I

R

K

E

E

A

Y

V

A

K

K

D

E

A

A

V

L

D

D

E

K

G

G

L

L

T

T

H

H

Y

Y

T

G

S

P

N

N

M

I

G

G

M

L

E

L

E

E

L

L

R

R

E

E

A

A

I

I

A

A

D

E

K

K

L

L

K

K

S

K

E

Q

N

N

R

G

V

I

H

E

A

A

E

D

P

P

E

K

S

T

-

E

I

I

I

M

V

V

T

L

V

L

G
|
G

A
|
A

S
x
N

E

Q

A

A

I

F

F

L

M

M

C

G

T

L

Q

S

A

A

L

F

L

L

D

K

I

D

G

G

D

E

H

E

A

V

L

L

I

I

P

P

D

T

P

P

G

A

F
|
F

L

V

S

S

Y

Y

D

A

A

P

C

A

V

V

R

I

L

L

S

A

G

G

A

G

V

K

S

P

I

V

P

E

V

V

P

P

L

T

S

Y

M

E

D

E

E

D

G

E

F

F

S

R

M

L

S

N

P

V

E

D

R

E

V

L

E

K

S

K

L

Y

I

V

T

T

Q

D

D

K

T

T

R

R

V

A

I

L

I

I

M

I

N

N

S

S

P

P

S

C

N
|
N

P

P

T

T

G

G

S

A

V

V

M

L

G

T

K

K

D

K

D

D

V

L

K

E

G

E

I

I

A

A

E

D

I

F

A

V

E

V

D

E

N

H

D

D

L

L

I

I

I

V

I

I

S

S

D
|
D

E

E

I
x
V

Y
|
Y

E

E

K

H

I

F

I

I

Y

Y

-

D

D

D

G

A

K

R

H

H

Y

Y

S

S

P

I

A

A

Q

S

F

L

T

D

-

G

-

M

-

F

D

E

N

R

A

T

L

I

I

T

V

V

N

N

G

G

F

F

S
|
S

K
|
K

T

T

Y

F

A

A

M

M

T

T

G

G

L

W

R
|
R

I

L

G

G

Y

F

V

V

A

A

G

A

C

P

E

S

D

W

I

I

I

I

E

E

E

R

L

M

L

V

K

K

V

F

H

Q

Q

M

Y

Y

N

N

T

A

A

T

C

C

A

P

P

V

S

T

I

F

S

I

Q

Q

Y

Y

A

A

A

A

L

A

A

K

A

A

I

L

R

K

G

D

P

E

Q

R

N

S

C

W

-

K

-

A

V

V

K

E

D

E

M

M

V

R

D

K

E

E

F

Y

R

D

R

R

R

R

R

R

D

K

L

L

M

V

F

W

R

K

S

R

L

L

T

N

D

E

M

M

G

G

L

L

E

P

C

T

V

V

L

K

P

P

G

K

G

G

A

A

F

F

Y

Y

M

I

F

F

P

P

Y

R

A

I

G

R

D

D

-

T

-

G

-

L

-

T

S

S

E

K

E

K

F

F

T

S

K

E

L

L

S

M

L

L

-

K

E

E

A

A

G

R

V

V

A

A

V

V

V

V

P

P

G

G

S

S

A

A

F

F

G

G

N

K

E

A

G

G

K

E

G

G

Y

Y

I

V

R

R

M

I

S

S

Y

Y

A

A

T

T

S

A

Y

Y

E

E

L

K

I

L

E

E

E

E

A

A

M

M

E

D

R

R

L

M

K

E

T

R

V

V

C

L

active site: K232 (= K227)
binding pyridoxal-5'-phosphate: G94 (= G93), A95 (= A94), N96 (≠ S95), F120 (= F119), N170 (= N169), D198 (= D197), V200 (≠ I199), Y201 (= Y200), S231 (= S226), K232 (= K227), R240 (= R235)

Q56232 Aspartate/prephenate aminotransferase; AspAT / PAT; Transaminase A; EC 2.6.1.1; EC 2.6.1.78 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see 3 papers)
40% identity, 100% coverage: 1:371/372 of query aligns to 1:383/385 of Q56232

query
sites
Q56232

M

M

R

R

K

G

F

L

A

S

S

R

R

R

V

V

K

Q

D

A

I

M

Q
x
K

L

P

S

S

E

A

I

T

R

V

K

A

I

V

F

N

E

A

V

K

A

A

D

L

E

E

-

L

-

R

-

R

-

Q

-

G

-

V

D

D

T

L

I

V

N

A

L

L

G

T

I

A

G

G

E

E

P

P

D

D

F

F

S

D

V

T

P

P

D

E

H

H

V

V

R

K

E

E

A

A

V

A

K

R

D

R

A

A

V

L

D

A

E

Q

G

G

L

K

T

T

H

K

Y

Y

T

A

S

P

N

P

M

A

G

G

M

I

E

P

E

E

L

L

R

R

E

E

A

A

I

L

A

A

D

E

K

K

L

F

K

R

S

R

E

E

N

N

R

G

V

L

H

S

A

V

E

T

P

P

E

E

S

E

I

T

I

I

V

V

T

T

V

V

G

G

A

G

S

K

E

Q

A

A

I

L

F

F

M

N

C

L

T

F

Q

Q

A

A

L

I

L

L

D

D

I

P

G

G

D

D

H

E

A

V

L

I

I

V

P

L

D

S

P

P

G

Y

F

W

L

V

S

S

Y

Y

D

P

A

E

C

M

V

V

R

R

L

F

S

A

G

G

A

G

V

V

S

V

I

V

P

E

V

V

P

E

L

T

S

L

M

P

D

E

E

E

G

G

F

F

S

V

M

P

S

D

P

P

E

E

R

R

V

V

E

R

S

R

L

A

I

I

T

T

Q

P

D

R

T

T

R

K

V

A

I

L

I

V

M

V

N

N

S

S

P

P

S

N

N

N

P

P

T

T

G

G

S

A

V

V

M

Y

G

P

K

K

D

E

D

V

V

L

K

E

G

A

I

L

A

A

E

R

I

L

A

A

E

V

D

E

N

H

D

D

L

F

I

Y

I

L

I

V

S

S

D

D

E

E

I

I

Y

Y

E

E

K

H

I

L

I

L

Y

Y

D

E

G

G

K

E

H

H

Y

F

S

S

P

P

A

G

Q

R

F

V

T

A

-

P

D

E

N

H

A

T

L

L

I

T

V

V

N

N

G

G

F

A

S

A

K
|
K

T

A

Y

F

A

A

M

M

T

T

G

G

L

W

R

R

I

I

G

G

Y

Y

V

A

A

C

G

G

C

P

E

K

D

E

I

V

I

I

E

K

E

A

L

M

L

A

K

S

V

V

H

S

Q

S

Y

Q

N

S

T

T

A

T

C

S

A

P

P

D

S

T

I

I

S

A

Q

Q

Y

W

A

A

A

T

L

L

A

E

A

A

I

L

R

T

G

N

P

-

Q

Q

N

E

C

A

V

S

K

R

D

A

M

F

V

V

-

E

-

M

-

A

-

R

D

E

E

A

F

Y

R

R

R

R

R

R

R

R

D

D

L

L

M

L

F

L

R

E

S

G

L

L

T

T

D

A

M

L

G

G

L

L

E

K

C

A

V

V

L

R

P

P

G

S

G

G

A

A

F

F

Y

Y

M

V

F

L

-

M

-

D

-

T

-

S

P

P

Y

I

A

A

G

P

D

D

S

E

E

V

E

R

F

A

T

A

K

E

L

R

S

L

L

L

E

E

A

A

G

G

V

V

A

A

V

V

V

V

P

P

G

G

S

T

A

D

F

F

G

A

N

-

E

-

G

A

K

F

G

G

Y

H

I

V

R

R

M

L

S

S

Y

Y

A

A

T

T

S

S

Y

E

E

E

L

N

I

L

E

R

E

K

A

A

M

L

E

E

R

R

L

F

K

A

T

R

V

V

C

L

G

G

K12 (≠ Q12) Important for prephenate aminotransferase activity; mutation to G: 10-fold increase in Km for prephenate. Does not affect Km for oxaloacetate.
K234 (= K227) modified: N6-(pyridoxal phosphate)lysine

1b5oA Thermus thermophilus aspartate aminotransferase single mutant 1 (see paper)
41% identity, 99% coverage: 1:370/372 of query aligns to 1:382/382 of 1b5oA

query
sites
1b5oA

M

M

R

R

K

G

F

L

A

S

S

R

R

R

V

V

K

Q

D

A

I

M

Q

K

L

P

S

S

E

A

I

T

R

V

K

A

I

V

F

N

E

A

V

K

A

A

D

L

E

E

-

L

-

R

-

R

-

Q

-

G

-

V

D

D

T

L

I

V

N

A

L

L

G

T

I

A

G

G

E

E

P

P

D

D

F

F

S

D

V

T

P

P

D

E

H

H

V

V

R

K

E

E

A

A

V

A

K

R

D

R

A

A

V

L

D

A

E

Q

G

G

L

K

T

T

H

K

Y

Y

T

A

S

P

N

P

M

A

G

G

M

I

E

P

E

E

L

L

R

R

E

E

A

A

I

L

A

A

D

E

K

K

L

F

K

R

S

R

E

E

N

N

R

G

V

L

H

S

A

V

E

T

P

P

E

E

S

E

I

T

I

I

V

V

T

T

V

V

G
|
G

A
x
G

S
|
S

E

Q

A

A

I

L

F

F

M

N

C

L

T

F

Q

Q

A

A

L

I

L

L

D

D

I

P

G

G

D

D

H

E

A

V

L

I

I

V

P

L

D

S

P

P

G

Y

F
x
W

L

V

S

S

Y

Y

D

P

A

E

C

M

V

V

R

R

L

F

S

A

G

G

A

G

V

V

S

V

I

V

P

E

V

V

P

E

L

T

S

L

M

P

D

E

E

E

G

G

F

F

S

V

M

P

S

D

P

P

E

E

R

R

V

V

E

R

S

R

L

A

I

I

T

T

Q

P

D

R

T

T

R

K

V

A

I

L

I

V

M

V

N

N

S

S

P

P

S

N

N
|
N

P

P

T

T

G

G

S

A

V

V

M

Y

G

P

K

K

D

E

D

V

V

L

K

E

G

A

I

L

A

A

E

R

I

L

A

A

E

V

D

E

N

H

D

D

L

F

I

Y

I

L

I

V

S

S

D
|
D

E

E

I
|
I

Y
|
Y

E

E

K

H

I

L

I

L

Y

Y

D

E

G

G

K

E

H

H

Y

F

S

S

P

P

A

G

Q

R

F

V

T

A

-

P

D

E

N

H

A

T

L

L

I

T

V

V

N

N

G

G

F

A

S
x
A

K
|
K

T

A

Y

F

A

A

M

M

T

T

G

G

L

W

R
|
R

I

I

G

G

Y

Y

V

A

A

C

G

G

C

P

E

K

D

E

I

V

I

I

E

K

E

A

L

M

L

A

K

S

V

V

H

S

Q

S

Y

Q

N

S

T

T

A

T

C

S

A

P

P

D

S

T

I

I

S

A

Q

Q

Y

W

A

A

A

T

L

L

A

E

A

A

I

L

R

T

G

N

P

-

Q

Q

N

E

C

A

V

S

K

R

D

A

M

F

V

V

-

E

-

M

-

A

-

R

D

E

E

A

F

Y

R

R

R

R

R

R

R

R

D

D

L

L

M

L

F

L

R

E

S

G

L

L

T

T

D

A

M

L

G

G

L

L

E

K

C

A

V

V

L

R

P

P

G

S

G

G

A

A

F

F

Y

Y

M

V

F

L

-

M

-

D

-

T

-

S

P

P

Y

I

A

A

G

P

D

D

S

E

E

V

E

R

F

A

T

A

K

E

L

R

S

L

L

L

E

E

A

A

G

G

V

V

A

A

V

V

V

V

P

P

G

G

S

T

A

D

F

F

G

A

N

-

E

-

G

A

K

F

G

G

Y

H

I

V

R

R

M

L

S

S

Y

Y

A

A

T

T

S

S

Y

E

E

E

L

N

I

L

E

R

E

K

A

A

M

L

E

E

R

R

L

F

K

A

T

R

V

V

C

L

active site: W125 (≠ F119), D203 (= D197), I205 (= I199), K234 (= K227)
binding pyridoxal-5'-phosphate: G99 (= G93), G100 (≠ A94), S101 (= S95), W125 (≠ F119), N175 (= N169), D203 (= D197), I205 (= I199), Y206 (= Y200), A233 (≠ S226), K234 (= K227), R242 (= R235)

1gc4A Thermus thermophilus aspartate aminotransferase tetra mutant 2 complexed with aspartate (see paper)
40% identity, 99% coverage: 1:370/372 of query aligns to 1:382/382 of 1gc4A

query
sites
1gc4A

M

M

R

R

K

G

F

L

A

S

S

R

R

R

V

V

K

Q

D

A

I

M

Q

K

L

P

S

D

E

A

I

V

R

V

K

A

I

V

F

N

E

A

V

K

A

A

D

L

E

E

-

L

-

R

-

R

-

Q

-

G

-

V

D

D

T

L

I

V

N

A

L

L

G

T

I

A

G

G

E

E

P

P

D

D

F

F

S

D

V

T

P

P

D

E

H

H

V

V

R

K

E

E

A

A

V

A

K

R

D

R

A

A

V

L

D

A

E

Q

G

G

L

K

T

T

H

K

Y
|
Y

T

A

S

P

N

P

M

A

G

G

M

I

E

P

E

E

L

L

R

R

E

E

A

A

I

L

A

A

D

E

K

K

L

F

K

R

S

R

E

E

N

N

R

G

V

L

H

S

A

V

E

T

P

P

E

E

S

E

I

T

I

I

V

V

T

T

V

V

G
|
G

A
x
G

S
|
S

E

Q

A

A

I

L

F

F

M

N

C

L

T

F

Q

Q

A

A

L

I

L

L

D

D

I

P

G

G

D

D

H

E

A

V

L

I

I

V

P

L

D

S

P

P

G

Y

F
x
W

L

V

S

S

Y

Y

D

P

A

E

C

M

V

V

R

R

L

F

S

A

G

G

A

G

V

V

S

V

I

V

P

E

V

V

P

E

L

T

S

L

M

P

D

E

E

E

G

G

F

F

S

V

M

P

S

D

P

P

E

E

R

R

V

V

E

R

S

R

L

A

I

I

T

T

Q

P

D

R

T

T

R

K

V

A

I

L

I

V

M

V

N

N

S

S

P

P

S

N

N
|
N

P

P

T

T

G

G

S

A

V

V

M

Y

G

P

K

K

D

E

D

V

V

L

K

E

G

A

I

L

A

A

E

R

I

L

A

A

E

V

D

E

N

H

D

D

L

F

I

Y

I

L

I

V

S

S

D
|
D

E

E

I

I

Y
|
Y

E

E

K

H

I

L

I

L

Y

Y

D

E

G

G

K

E

H

H

Y

F

S

S

P

P

A

G

Q

R

F

V

T

A

-

P

D

E

N

H

A

T

L

L

I

T

V

V

N

N

G

G

F

A

S

A

K

K

T

A

Y

F

A

A

M

M

T

T

G

G

L

W

R
|
R

I

I

G

G

Y

Y

V

A

A

C

G

G

C

P

E

K

D

E

I

V

I

I

E

K

E

A

L

M

L

A

K

S

V

V

H

S

Q
x
R

Y

Q

N

S

T

T

A

T

C

S

A

P

P

D

S

T

I

I

S

A

Q

Q

Y

W

A

A

A

T

L

L

A

E

A

A

I

L

R

T

G

N

P

-

Q

Q

N

E

C

A

V

S

K

R

D

A

M

F

V

V

-

E

-

M

-

A

-

R

D

E

E

A

F

Y

R

R

R

R

R

R

R

R

D

D

L

L

M

L

F

L

R

E

S

G

L

L

T

T

D

A

M

L

G

G

L

L

E

K

C

A

V

V

L

R

P

P

G

S

G

G

A

A

F

F

Y
|
Y

M

V

F

L

-

M

-

D

-

T

-

S

P

P

Y

I

A

A

G

P

D

D

S

E

E

V

E

R

F

A

T

A

K

E

L

R

S

L

L

L

E

E

A

A

G

G

V

V

A

A

V

V

V

V

P

P

G

G

S

T

A

D

F

F

G

A

N

-

E

-

G

A

K

F

G

G

Y

H

I

V

R
|
R

M

L

S

S

Y

Y

A

A

T

T

S

S

Y

E

E

E

L

N

I

L

E

R

E

K

A

A

M

L

E

E

R

R

L

F

K

A

T

R

V

V

C

L

binding aspartic acid: Y64 (= Y58), W125 (≠ F119), N175 (= N169), R261 (≠ Q254), Y322 (= Y312), R361 (= R349)
binding pyridoxal-5'-phosphate: G99 (= G93), G100 (≠ A94), S101 (= S95), W125 (≠ F119), N175 (= N169), D203 (= D197), Y206 (= Y200), R242 (= R235)

1gc3A Thermus thermophilus aspartate aminotransferase tetra mutant 2 complexed with tryptophan (see paper)
40% identity, 99% coverage: 1:370/372 of query aligns to 1:382/382 of 1gc3A

query
sites
1gc3A

M

M

R

R

K

G

F

L

A

S

S

R

R

R

V

V

K

Q

D

A

I

M

Q

K

L

P

S

D

E

A

I
x
V

R

V

K

A

I

V

F
x
N

E

A

V

K

A

A

D

L

E

E

-

L

-

R

-

R

-

Q

-

G

-

V

D

D

T

L

I

V

N

A

L

L

G
x
T

I
x
A

G
|
G

E
|
E

P

P

D

D

F

F

S

D

V

T

P

P

D

E

H

H

V

V

R

K

E

E

A

A

V

A

K

R

D

R

A

A

V

L

D

A

E

Q

G

G

L

K

T

T

H

K

Y

Y

T

A

S

P

N

P

M

A

G

G

M

I

E

P

E

E

L

L

R

R

E

E

A

A

I

L

A

A

D

E

K

K

L

F

K

R

S

R

E

E

N

N

R

G

V

L

H

S

A

V

E

T

P

P

E

E

S

E

I

T

I

I

V

V

T

T

V

V

G

G

A
x
G

S
|
S

E

Q

A

A

I

L

F

F

M

N

C

L

T

F

Q

Q

A

A

L

I

L

L

D

D

I

P

G

G

D

D

H

E

A

V

L

I

I

V

P

L

D

S

P

P

G

Y

F
x
W

L

V

S

S

Y

Y

D

P

A

E

C

M

V

V

R

R

L

F

S

A

G

G

A

G

V

V

S

V

I

V

P

E

V

V

P

E

L

T

S

L

M

P

D

E

E

E

G

G

F

F

S

V

M

P

S

D

P

P

E

E

R

R

V

V

E

R

S

R

L

A

I

I

T

T

Q

P

D

R

T

T

R

K

V

A

I

L

I

V

M

V

N

N

S

S

P

P

S

N

N
|
N

P

P

T

T

G

G

S

A

V

V

M

Y

G

P

K

K

D

E

D

V

V

L

K

E

G

A

I

L

A

A

E

R

I

L

A

A

E

V

D

E

N

H

D

D

L

F

I

Y

I

L

I

V

S

S

D
|
D

E

E

I

I

Y
|
Y

E

E

K

H

I

L

I

L

Y

Y

D

E

G

G

K

E

H

H

Y

F

S

S

P

P

A

G

Q

R

F

V

T

A

-

P

D

E

N

H

A

T

L

L

I

T

V

V

N

N

G

G

F

A

S

A

K
|
K

T

A

Y

F

A

A

M

M

T

T

G

G

L

W

R
|
R

I

I

G

G

Y

Y

V

A

A

C

G

G

C

P

E

K

D

E

I

V

I

I

E

K

E

A

L

M

L

A

K

S

V

V

H

S

Q

R

Y

Q

N

S

T

T

A

T

C

S

A

P

P

D

S

T

I

I

S

A

Q

Q

Y

W

A

A

A

T

L

L

A

E

A

A

I

L

R

T

G

N

P

-

Q

Q

N

E

C

A

V

S

K

R

D

A

M

F

V

V

-

E

-

M

-

A

-

R

D

E

E

A

F

Y

R

R

R

R

R

R

R

R

D

D

L

L

M

L

F

L

R

E

S

G

L

L

T

T

D

A

M

L

G

G

L

L

E

K

C

A

V

V

L

R

P

P

G

S

G

G

A

A

F

F

Y
|
Y

M

V

F

L

-

M

-

D

-

T

-

S

P

P

Y

I

A

A

G

P

D

D

S

E

E

V

E

R

F

A

T

A

K

E

L

R

S

L

L

L

E

E

A

A

G

G

V

V

A

A

V

V

V

V

P

P

G

G

S

T

A

D

F

F

G

A

N

-

E

-

G

A

K

F

G

G

Y

H

I

V

R
|
R

M

L

S

S

Y

Y

A

A

T

T

S

S

Y

E

E

E

L

N

I

L

E

R

E

K

A

A

M

L

E

E

R

R

L

F

K

A

T

R

V

V

C

L

binding pyridoxal-5'-phosphate: G100 (≠ A94), S101 (= S95), W125 (≠ F119), N175 (= N169), D203 (= D197), Y206 (= Y200), K234 (= K227), R242 (= R235)
binding tryptophan: V16 (≠ I16), N20 (≠ F20), T37 (≠ G31), A38 (≠ I32), G39 (= G33), E40 (= E34), Y322 (= Y312), R361 (= R349)

1bkgA Aspartate aminotransferase from thermus thermophilus with maleate (see paper)
40% identity, 99% coverage: 1:370/372 of query aligns to 1:382/382 of 1bkgA

query
sites
1bkgA

M

M

R

R

K

G

F

L

A

S

S

R

R

R

V

V

K

Q

D

A

I

M

Q

K

L

P

S

S

E

A

I

T

R

V

K

A

I

V

F

N

E

A

V

K

A

A

D

L

E

E

-

L

-

R

-

R

-

Q

-

G

-

V

D

D

T

L

I

V

N

A

L

L

G

T

I

A

G

G

E

E

P

P

D

D

F

F

S

D

V

T

P

P

D

E

H

H

V

V

R

K

E

E

A

A

V

A

K

R

D

R

A

A

V

L

D

A

E

Q

G

G

L

K

T

T

H

K

Y

Y

T

A

S

P

N

P

M

A

G

G

M

I

E

P

E

E

L

L

R

R

E

E

A

A

I

L

A

A

D

E

K

K

L

F

K

R

S

R

E

E

N

N

R

G

V

L

H

S

A

V

E

T

P

P

E

E

S

E

I

T

I

I

V

V

T

T

V

V

G
|
G

A
x
G

S
x
K

E

Q

A

A

I

L

F

F

M

N

C

L

T

F

Q

Q

A

A

L

I

L

L

D

D

I

P

G

G

D

D

H

E

A

V

L

I

I

V

P

L

D

S

P

P

G

Y

F
x
W

L

V

S

S

Y

Y

D

P

A

E

C

M

V

V

R

R

L

F

S

A

G

G

A

G

V

V

S

V

I

V

P

E

V

V

P

E

L

T

S

L

M

P

D

E

E

E

G

G

F

F

S

V

M

P

S

D

P

P

E

E

R

R

V

V

E

R

S

R

L

A

I

I

T

T

Q

P

D

R

T

T

R

K

V

A

I

L

I

V

M

V

N
|
N

S

S

P

P

S

N

N
|
N

P

P

T

T

G

G

S

A

V

V

M

Y

G

P

K

K

D

E

D

V

V

L

K

E

G

A

I

L

A

A

E

R

I

L

A

A

E

V

D

E

N

H

D

D

L

F

I

Y

I

L

I

V

S

S

D
|
D

E

E

I
|
I

Y
|
Y

E

E

K

H

I

L

I

L

Y

Y

D

E

G

G

K

E

H

H

Y

F

S

S

P

P

A

G

Q

R

F

V

T

A

-

P

D

E

N

H

A

T

L

L

I

T

V

V

N

N

G

G

F

A

S

A

K
|
K

T

A

Y

F

A

A

M

M

T

T

G

G

L

W

R
|
R

I

I

G

G

Y

Y

V

A

A

C

G

G

C

P

E

K

D

E

I

V

I

I

E

K

E

A

L

M

L

A

K

S

V

V

H

S

Q

S

Y

Q

N

S

T

T

A

T

C

S

A

P

P

D

S

T

I

I

S

A

Q

Q

Y

W

A

A

A

T

L

L

A

E

A

A

I

L

R

T

G

N

P

-

Q

Q

N

E

C

A

V

S

K

R

D

A

M

F

V

V

-

E

-

M

-

A

-

R

D

E

E

A

F

Y

R

R

R

R

R

R

R

R

D

D

L

L

M

L

F

L

R

E

S

G

L

L

T

T

D

A

M

L

G

G

L

L

E

K

C

A

V

V

L

R

P

P

G

S

G

G

A

A

F

F

Y

Y

M

V

F

L

-

M

-

D

-

T

-

S

P

P

Y

I

A

A

G

P

D

D

S

E

E

V

E

R

F

A

T

A

K

E

L

R

S

L

L

L

E

E

A

A

G

G

V

V

A

A

V

V

V

V

P

P

G

G

S

T

A

D

F

F

G

A

N

-

E

-

G

A

K

F

G

G

Y

H

I

V

R

R

M

L

S

S

Y

Y

A

A

T

T

S

S

Y

E

E

E

L

N

I

L

E

R

E

K

A

A

M

L

E

E

R

R

L

F

K

A

T

R

V

V

C

L

active site: W125 (≠ F119), D203 (= D197), I205 (= I199), K234 (= K227)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G99 (= G93), G100 (≠ A94), K101 (≠ S95), W125 (≠ F119), N171 (= N165), N175 (= N169), D203 (= D197), I205 (= I199), Y206 (= Y200), K234 (= K227), R242 (= R235)

1bjwA Aspartate aminotransferase from thermus thermophilus (see paper)
40% identity, 99% coverage: 1:370/372 of query aligns to 1:382/382 of 1bjwA

query
sites
1bjwA

M

M

R

R

K

G

F

L

A

S

S

R

R

R

V

V

K

Q

D

A

I

M

Q

K

L

P

S

S

E

A

I

T

R

V

K

A

I

V

F

N

E

A

V

K

A

A

D

L

E

E

-

L

-

R

-

R

-

Q

-

G

-

V

D

D

T

L

I

V

N

A

L

L

G

T

I

A

G
|
G

E

E

P

P

D

D

F

F

S

D

V

T

P

P

D

E

H

H

V

V

R

K

E

E

A

A

V

A

K

R

D

R

A

A

V

L

D

A

E

Q

G

G

L

K

T

T

H

K

Y

Y

T

A

S

P

N

P

M

A

G

G

M

I

E

P

E

E

L

L

R

R

E

E

A

A

I

L

A

A

D

E

K

K

L

F

K

R

S

R

E

E

N

N

R

G

V

L

H

S

A

V

E

T

P

P

E

E

S

E

I

T

I

I

V

V

T

T

V

V

G

G

A

G

S

K

E

Q

A

A

I

L

F

F

M

N

C

L

T

F

Q

Q

A

A

L

I

L

L

D

D

I

P

G

G

D

D

H

E

A

V

L

I

I

V

P

L

D

S

P

P

G

Y

F
x
W

L

V

S

S

Y

Y

D

P

A

E

C

M

V

V

R

R

L

F

S

A

G

G

A

G

V

V

S

V

I

V

P

E

V

V

P

E

L

T

S

L

M

P

D

E

E

E

G

G

F

F

S

V

M

P

S

D

P

P

E

E

R

R

V

V

E

R

S

R

L

A

I

I

T

T

Q

P

D

R

T

T

R

K

V

A

I

L

I

V

M

V

N

N

S

S

P

P

S

N

N
|
N

P

P

T

T

G

G

S

A

V

V

M

Y

G

P

K

K

D

E

D

V

V

L

K

E

G

A

I

L

A

A

E

R

I

L

A

A

E

V

D

E

N

H

D

D

L

F

I

Y

I

L

I

V

S

S

D
|
D

E

E

I
|
I

Y

Y

E

E

K

H

I

L

I

L

Y

Y

D

E

G

G

K

E

H

H

Y

F

S

S

P

P

A

G

Q

R

F

V

T

A

-

P

D

E

N

H

A

T

L

L

I

T

V

V

N

N

G

G

F

A

S

A

K
|
K

T

A

Y

F

A

A

M

M

T

T

G

G

L

W

R

R

I

I

G

G

Y

Y

V

A

A

C

G

G

C

P

E

K

D

E

I

V

I

I

E

K

E

A

L

M

L

A

K

S

V

V

H

S

Q

S

Y

Q

N

S

T

T

A

T

C

S

A

P

P

D

S

T

I

I

S

A

Q

Q

Y

W

A

A

A

T

L

L

A

E

A

A

I

L

R

T

G

N

P

-

Q

Q

N

E

C

A

V

S

K

R

D

A

M

F

V

V

-

E

-

M

-

A

-

R

D

E

E

A

F

Y

R

R

R

R

R

R

R

R

D

D

L

L

M

L

F

L

R

E

S

G

L

L

T

T

D

A

M

L

G

G

L

L

E

K

C

A

V

V

L

R

P

P

G

S

G

G

A

A

F

F

Y

Y

M

V

F

L

-

M

-

D

-

T

-

S

P

P

Y

I

A

A

G

P

D

D

S

E

E

V

E

R

F

A

T

A

K

E

L

R

S

L

L

L

E

E

A

A

G

G

V

V

A

A

V

V

V

V

P

P

G

G

S

T

A

D

F

F

G

A

N

-

E

-

G

A

K

F

G

G

Y

H

I

V

R
|
R

M

L

S

S

Y

Y

A

A

T

T

S

S

Y

E

E

E

L

N

I

L

E

R

E

K

A

A

M

L

E

E

R

R

L

F

K

A

T

R

V

V

C

L

active site: W125 (≠ F119), D203 (= D197), I205 (= I199), K234 (= K227)
binding phosphate ion: G39 (= G33), W125 (≠ F119), N175 (= N169), R361 (= R349)

1j32A Aspartate aminotransferase from phormidium lapideum
38% identity, 98% coverage: 3:367/372 of query aligns to 2:381/388 of 1j32A

query
sites
1j32A

K

K

F

L

A

A

S

A

R

R

V

V

K

E

D

S

I

V

Q

S

L

P

S

S

E

M

I

T

-

L

-

I

-

I

-

D

-

A

-

K

R

A

K

K

I

A

F

M

E

K

V

A

A

E

D

G

E

I

D

D

T

V

I

C

N

S

L

F

G

S

I

A

G

G

E

E

P

P

D

D

F

F

S

N

V

T

P

P

D

K

H

H

V

I

R

V

E

E

A

A

V

A

K

K

D

A

A

A

V

L

D

E

E

Q

G

G

L

K

T

T

H

R

Y

Y

T

G

S

P

N

A

M

A

G

G

M

E

E

P

E

R

L

L

R

R

E

E

A

A

I

I

A

A

D

Q

K

K

L

L

K

Q

S

R

E

D

N

N

R

G

V

L

H

C

A

Y

E

G

P

A

E

D

S

N

I

I

I

L

V

V

T

T

V

N

G
|
G

A
x
G

S
x
K

E

Q

A

S

I

I

F

F

M

N

C

L

T

M

Q

L

A

A

L

M

L

I

D

E

I

P

G

G

D

D

H

E

A

V

L

I

I

I

P

P

D

A

P

P

G

F

F
x
W

L

V

S

S

Y

Y

D

P

A

E

C

M

V

V

R

K

L

L

S

A

G

E

A

G

V

T

S

P

I

V

P

I

V

L

P

P

L

T

S

T

M

V

D

E

E

T

G

Q

F

F

S

K

M

V

S

S

P

P

E

E

R

Q

V

I

E

R

S

Q

L

A

I

I

T

T

Q

P

D

K

T

T

R

K

V

L

I

L

I

V

M

F

N

N

S

T

P

P

S

S

N
|
N

P

P

T

T

G

G

S

M

V

V

M

Y

G

T

K

P

D

D

D

E

V

V

K

R

G

A

I

I

A

A

E

Q

I

V

A

A

E

V

D

E

N

A

D

G

L

L

I

W

I

V

I

L

S

S

D
|
D

E

E

I
|
I

Y
|
Y

E

E

K

K

I

I

I

L

Y

Y

D

D

G

D

K

A

H

Q

Y

H

-

L

-

S

-

I

-

G

-

A

-

A

S

S

P

P

A

E

Q

A

F

Y

T

-

D

E

N

R

A

S

L

V

I

V

V

C

N

S

G

G

F

F

S

A

K
|
K

T

T

Y

Y

A

A

M

M

T

T

G

G

L

W

R
|
R

I

V

G

G

Y

F

V

L

A

A

G

G

C

P

E

V

D

P

I

L

I

V

E

K

E

A

L

A

L

T

K

K

V

I

H

Q

Q

G

Y

H

N

S

T

T

A

S

C

N

A

V

P

C

S

T

I

F

S

A

Q

Q

Y

Y

A

G

A

A

L

I

A

A

A

A

I

Y

R

E

G

N

P

S

Q

Q

N

D

C

C

V

V

K

Q

D

E

M

M

V

L

D

A

E

A

F

F

R

A

R

E

R

R

R

R

D

R

L

Y

M

M

F

L

R

D

S

A

L

L

T

N

D

A

M

M

-

P

G

G

L

L

E

E

C

C

V

P

L

K

P

P

G

D

G

G

A

A

F

F

Y

Y

M

M

F

F

P

P

Y

S

A

I

G

A

-

K

-

T

-

G

-

R

D

S

S

S

E

L

E

D

F

F

-

C

T

S

K

E

L

L

S

L

L

D

E

Q

A

H

G

Q

V

V

A

A

V

T

V

V

P

P

G

G

S

A

A

A

F

F

G

G

N

A

E

D

G

-

K

-

G

D

Y

C

I

I

R

R

M

L

S

S

Y

Y

A

A

T

T

S

D

Y

L

E

D

L

T

I

I

E

K

E

R

A

G

M

M

E

E

R

R

L

L

K

E

active site: W124 (≠ F119), D202 (= D197), I204 (= I199), K237 (= K227)
binding pyridoxal-5'-phosphate: G98 (= G93), G99 (≠ A94), K100 (≠ S95), W124 (≠ F119), N174 (= N169), D202 (= D197), I204 (= I199), Y205 (= Y200), K237 (= K227), R245 (= R235)

Q58097 (5-formylfuran-3-yl)methyl phosphate transaminase; 4-HFC-P:alanine aminotransferase; EC 2.6.1.108 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
42% identity, 94% coverage: 20:367/372 of query aligns to 24:365/370 of Q58097

query
sites
Q58097

F

L

E

E

V

S

A

E

D

G

E

K

D

K

T

V

I

I

N

H

L

L

G

E

I

I

G

G

E

E

P

P

D

D

F

F

S

N

V

T

P

P

D

K

H

P

V

I

R

V

E

D

A

E

V

G

K

I

D

K

A

S

V

L

D

K

E

E

G

G

L

K

T

T

H

H

Y

Y

T

T

S

D

N

S

M

R

G

G

M

I

E

L

E

E

L

L

R

R

E

E

A

K

I

I

A

S

D

E

K

L

L

Y

K

K

S

D

E

K

N

Y

R

K

V

A

H

D

A

I

E

I

P

P

E

D

S

N

I

I

I

I

V

I

T

T

V

G

G

G

A

S

S

S

E

L

A

G

I

L

F

F

M

F

C

A

T

L

Q

S

A

S

L

I

L

I

D

D

I

D

G

G

D

D

H

E

A

V

L

L

I

I

P

Q

D

N

P

P

G

C

F

Y

L

P

S

C

Y

Y

D

K

A

N

C

F

V

I

R

R

L

F

S

L

G

G

A

A

V

-

S

-

I

-

P

-

V

K

P

P

L

V

S

F

M

C

D

D

E

-

G

-

F

-

S

-

M

F

S

T

P

V

E

E

R

S

V

L

E

E

S

E

L

A

I

L

T

S

Q

D

D

K

T

T

R

K

V

A

I

I

I

I

M

I

N

N

S

S

P

P

S

S

N

N

P

P

T

L

G

G

S

E

V

V

M

I

G

D

K

R

D

E

D

-

V

-

K

-

G

-

I

I

A

Y

E

E

I

F

A

A

E

Y

D

E

N

N

D

I

L

P

I

Y

I

I

I

I

S

S

D

D

E

E

I

I

Y

Y

E

N

K

G

I

L

I

V

Y

Y

D

E

G

G

K

K

H

C

Y

Y

S

S

P

A

A

I

Q

E

F

F

T

D

D

E

N

N

-

L

-

E

-

K

A

T

L

I

I

L

V

I

N

N

G

G

F

F

S

S

K
|
K

T

L

Y

Y

A

A

M

M

T

T

G

G

L

W

R

R

I

I

G

G

Y

Y

V

V

A

I

G

S

C

N

E

D

D

E

I

I

I

I

E

E

E

A

L

I

L

L

K

K

V

L

H

Q

Q

Q

Y

N

N

L

T

F

A

I

C

S

A

A

P

P

S

T

I

I

S

S

Q

Q

Y

Y

A

A

A

A

L

L

A

K

A

A

I

F

-

E

R

K

G

E

P

T

Q

E

N

R

C

E

V

I

K

N

D

S

M

M

V

I

D

K

E

E

F

F

R

D

R

R

R

R

R

R

D

R

L

L

M

V

F

L

R

K

S

Y

L

V

T

K

D

D

M

F

G

G

L

W

E

E

C

V

V

N

L

N

P

P

G

I

G

G

A

A

F

Y

Y

Y

M

V

F

F

P

P

Y

N

A

I

G

G

-

E

D

D

S

G

E

R

E

E

F

F

T

A

-

Y

K

K

L

L

S

L

L

K

E

E

A

K

G

F

V

V

A

A

V

L

V

T

P

P

G

G

S

I

A

G

F

F

G

G

N

S

E

K

G

G

K

K

G

N

Y

Y

I

I

R

R

M

I

S

S

Y

Y

A

A

T

N

S

S

Y

Y

E

E

L

N

I

I

E

K

E

E

A

G

M

L

E

E

R

R

L

I

K

K

K222 (= K227) modified: N6-(pyridoxal phosphate)lysine

P58350 Aspartate aminotransferase; AAT; AspAT; Putative 2-aminoadipate transaminase; Transaminase A; EC 2.6.1.1; EC 2.6.1.39 from Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) (see paper)
39% identity, 98% coverage: 5:370/372 of query aligns to 15:406/410 of P58350

query
sites
P58350

A

A

S

S

R

R

V

I

K

S

D

S

I

I

Q

G

L

V

S

S

E

E

I

I

R

L

K

K

I

I

F

G

E

A

V

R

A

A

-

A

-

A

-

M

-

K

-

R

-

E

D

G

E

K

D

P

T

V

I

I

N

I

L

L

G

G

I

A

G

G

E

E

P

P

D

D

F

F

S

D

V

T

P

P

D

E

H

H

V

V

R

K

E

Q

A

A

V

A

K

S

D

D

A

A

V

I

D

H

E

R

G

G

L

E

T

T

H

K

Y

Y

T

T

S

A

N

L

M

D

G

G

M

T

E

P

E

E

L

L

R

K

E

K

A

A

I

I

A

R

D

E

K

K

L

F

K

Q

S

R

E

E

N

N

R

G

V

L

H

A

A

Y

E

E

P

L

E

D

S

E

I

I

I

T

V

V

T

A

V

T

G

G

A

A

S

K

E

Q

A

I

I

L

F

F

M

N

C

A

T

M

Q

M

A

A

L

S

L

L

D

D

I

P

G

G

D

D

H

E

A

V

L

I

I

I

P

P

D

T

P

P

G

Y

F

W

L

T

S

S

Y

Y

D

S

A

D

C

I

V

V

R

H

L

I

S

C

G

E

A

G

V

K

S

P

I

V

P

L

V

I

P

A

L

C

S

D

M

A

D

S

E

S

G

G

F

F

S

R

M

L

S

T

P

A

E

E

R

K

V

L

E

E

S

A

L

A

I

I

T

T

Q

P

D

R

T

T

R

R

V

W

I

V

I

L

M

L

N

N

S

S

P

P

S

S

N

N

P

P

T

S

G

G

S

A

V

A

M

Y

G

S

K

A

D

A

D

D

V

Y

K

R

G

P

I

L

A

L

E

E

I

V

-

L

A

L

E

R

D

H

N

P

D

H

L

V

I

W

I

L

I

L

S

V

D

D

E

D

I

M

Y

Y

E

E

K

H

I

I

I

V

Y

Y

D

D

G

G

K

F

H

R

Y

F

-

V

S

T

P

P

A

A

Q

Q

-

L

-

E

-

P

-

G

F

L

T

K

D

N

N

R

A

T

L

L

I

T

V

V

N

N

G

G

F

V

S

S

K
|
K

T

A

Y

Y

A

A

M

M

T

T

G

G

L

W

R

R

I

I

G

G

Y

Y

V

A

A

G

G

G

C

P

E

R

D

E

I

L

I

I

E

K

E

A

L

M

L

A

K

V

V

V

H

Q

Q

S

Y

Q

N

A

T

T

A

S

C

C

A

P

P

S

S

S

I

I

S

S

Q

Q

Y

A

A

A

A

S

L

V

A

A

A

A

I

L

R

N

G

G

P

P

Q

Q

N

D

C

F

V

L

K

K

D

E

M

R

V

T

D

E

E

S

F

F

R

Q

R

R

R

R

R

R

D

D

L

L

M

V

F

V

R

N

S

G

L

L

T

N

D

A

M

I

-

D

G

G

L

L

E

D

C

C

V

R

L

V

P

P

G

E

G

G

A

A

F

F

Y

Y

M

T

F

F

P

S

-

G

Y

C

A

A

G

G

-

V

-

L

-

G

-

K

-

V

-

T

-

P

-

S

-

G

-

K

-

R

-

I

-

K

-

T

D

D

S

T

E

D

E

F

F

C

T

A

K

Y

L

L

S

L

L

E

E

D

A

A

G

H

V

V

A

A

V

V

V

V

P

P

G

G

S

S

A

A

F

F

G

G

N

L

E

S

G

-

K

-

G

P

Y

F

I

F

R

R

M

I

S

S

Y

Y

A

A

T

T

S

S

Y

E

E

A

L

E

I

L

E

K

E

E

A

A

M

L

E

E

R

R

L

I

K

A

T

A

V

A

C

C

K249 (= K227) modified: N6-(pyridoxal phosphate)lysine

6f35A Crystal structure of the aspartate aminotranferase from rhizobium meliloti (see paper)
39% identity, 98% coverage: 5:370/372 of query aligns to 5:396/400 of 6f35A

query
sites
6f35A

A

A

S

S

R

R

V

I

K

S

D

S

I

I

Q

G

L

V

S

S

E

E

I

I

R

L

K

K

I

I

F

G

E

A

V

R

A

A

-

A

-

A

-

M

-

K

-

R

-

E

D

G

E

K

D

P

T

V

I

I

N

I

L

L

G

G

I

A

G

G

E

E

P

P

D

D

F

F

S

D

V

T

P

P

D

E

H

H

V

V

R

K

E

Q

A

A

V

A

K

S

D

D

A

A

V

I

D

H

E

R

G

G

L

E

T

T

H

K

Y

Y

T

T

S

A

N

L

M

D

G

G

M

T

E

P

E

E

L

L

R

K

E

K

A

A

I

I

A

R

D

E

K

K

L

F

K

Q

S

R

E

E

N

N

R

G

V

L

H

A

A

Y

E

E

P

L

E

D

S

E

I

I

I

T

V

V

T

A

V

T

G
|
G

A
|
A

S
x
K

E

Q

A

I

I

L

F

F

M

N

C

A

T

M

Q

M

A

A

L

S

L

L

D

D

I

P

G

G

D

D

H

E

A

V

L

I

I

I

P

P

D

T

P

P

G

Y

F

W

L

T

S

S

Y

Y

D

S

A

D

C

I

V

V

R

H

L

I

S

C

G

E

A

G

V

K

S

P

I

V

P

L

V

I

P

A

L

C

S

D

M

A

D

S

E

S

G

G

F

F

S

R

M

L

S

T

P

A

E

E

R

K

V

L

E

E

S

A

L

A

I

I

T

T

Q

P

D

R

T

T

R

R

V

W

I

V

I

L

M

L

N

N

S

S

P

P

S

S

N

N

P

P

T

S

G

G

S

A

V

A

M

Y

G

S

K

A

D

A

D

D

V

Y

K

R

G

P

I

L

A

L

E

E

I

V

-

L

A

L

E

R

D

H

N

P

D

H

L

V

I

W

I

L

I

L

S

V

D
|
D

E

D

I
x
M

Y
|
Y

E

E

K

H

I

I

I

V

Y

Y

D

D

G

G

K

F

H

R

Y

F

-

V

S

T

P

P

A

A

Q

Q

-

L

-

E

-

P

-

G

F

L

T

K

D

N

N

R

A

T

L

L

I

T

V

V

N

N

G

G

F

V

S
|
S

K
|
K

T

A

Y

Y

A

A

M

M

T

T

G

G

L

W

R
|
R

I

I

G

G

Y

Y

V

A

A

G

G

G

C

P

E

R

D

E

I

L

I

I

E

K

E

A

L

M

L

A

K

V

V

V

H

Q

Q

S

Y

Q

N

A

T

T

A

S

C

C

A

P

P

S

S

S

I

I

S

S

Q

Q

Y

A

A

A

A

S

L

V

A

V

A

A

I

L

R

N

G

G

P

P

Q

Q

N

D

C

F

V

L

K

K

D

E

M

R

V

T

D

E

E

S

F

F

R

Q

R

R

R

R

R

R

D

D

L

L

M

V

F

V

R

N

S

G

L

L

T

N

D

A

M

I

-

D

G

G

L

L

E

D

C

C

V

R

L

V

P

P

G

E

G

G

A

A

F

F

Y

Y

M

T

F

F

P

S

-

G

Y

C

A

A

G

G

-

V

-

L

-

G

-

K

-

V

-

T

-

P

-

S

-

G

-

K

-

R

-

I

-

K

-

T

D

D

S

T

E

D

E

F

F

C

T

A

K

Y

L

L

S

L

L

E

E

D

A

A

G

H

V

V

A

A

V

V

V

V

P

P

G

G

S

S

A

A

F

F

G

G

N

L

E

S

G

-

K

-

G

P

Y

F

I

F

R

R

M

I

S

S

Y

Y

A

A

T

T

S

S

Y

E

E

A

L

E

I

L

E

K

E

E

A

A

M

L

E

E

R

R

L

I

K

A

T

A

V

A

C

C

binding pyridoxal-5'-phosphate: G99 (= G93), A100 (= A94), K101 (≠ S95), D204 (= D197), M206 (≠ I199), Y207 (= Y200), S238 (= S226), K239 (= K227), R247 (= R235)

P14909 Aspartate aminotransferase; AspAT; Transaminase A; EC 2.6.1.1 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see 3 papers)