SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_048066055.1 NCBI__GCF_000007345.1:WP_048066055.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q5SHH5 [LysW]-aminoadipate semialdehyde transaminase; EC 2.6.1.118 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
44% identity, 96% coverage: 15:392/395 of query aligns to 9:390/395 of Q5SHH5

query
sites
Q5SHH5

Y

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GT 113:114 (≠ GA 117:118) binding pyridoxal 5'-phosphate
K254 (= K259) modified: N6-(pyridoxal phosphate)lysine
T283 (= T286) binding pyridoxal 5'-phosphate

1wkhA Acetylornithine aminotransferase from thermus thermophilus hb8
44% identity, 96% coverage: 15:392/395 of query aligns to 1:382/387 of 1wkhA

query
sites
1wkhA

Y

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active site: S13 (≠ M27), F132 (= F144), E184 (= E197), D217 (= D230), Q220 (= Q233), K246 (= K259), T275 (= T286), R363 (= R373)
binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: Y46 (≠ I60), S104 (= S116), G105 (= G117), T106 (≠ A118), F132 (= F144), S133 (≠ H145), E184 (= E197), E189 (= E202), D217 (= D230), I219 (≠ V232), K246 (= K259), R363 (= R373)

1wkgA Acetylornithine aminotransferase from thermus thermophilus hb8
44% identity, 96% coverage: 15:392/395 of query aligns to 1:382/387 of 1wkgA

query
sites
1wkgA

Y

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active site: S13 (≠ M27), F132 (= F144), E184 (= E197), D217 (= D230), Q220 (= Q233), K246 (= K259), T275 (= T286), R363 (= R373)
binding n~2~-acetyl-n~5~-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-ornithine: Y16 (= Y30), Y46 (≠ I60), G105 (= G117), T106 (≠ A118), F132 (= F144), S133 (≠ H145), R135 (= R147), E184 (= E197), D217 (= D230), I219 (≠ V232), Q220 (= Q233), K246 (= K259), G273 (≠ A284), T274 (≠ S285), T275 (= T286)

1vefA Acetylornithine aminotransferase from thermus thermophilus hb8
44% identity, 96% coverage: 15:392/395 of query aligns to 1:382/387 of 1vefA

query
sites
1vefA

Y

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V

V

V

V

K

E

A

A

I

V

Q

K

A

R

Q

Q

A

A

E

E

N

T

L

L

I

M

H

A

V

M

S

P

N

Q

L

T

Y

L

Y

P

T

T

E

P

I

M

Q

R

A

G

E

E

F

F

A

Y

E

R

T

T

L

L

A

T

S

A

I

I

T

L

G

P

M

P

E

E

-

L

-

N

R

R

V

V

F

F

F

P

C

V

N

N

S

S

G
|
G

A
x
T

E

E

S

A

V

N

E

E

A

A

A

A

M

L

K

K

L

F

A

A

R

R

V

A

A

H

T

T

G

G

K

R

S

K

A

K

F

F

V

V

A

A

A

A

E

M

H

R

S

G

F
|
F

H
x
S

G

G

R

R

T

T

I

M

G

G

A

S

L

L

S

S

V

V

T

T

H

W

K

E

S

P

M

K

Y

Y

R

R

D

E

P

P

F

F

M

L

P

P

P

L

V

V

S

E

S

P

E

-

T

V

T

E

F

F

V

I

P

P

Y

Y

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N

D

D

A

V

E

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A

A

I

L

R

K

Q

R

A

A

I

V

S

D

E

E

N

E

T

T

A

A

A

A

V

V

I

I

L

L

E
|
E

P

P

I

V

Q

Q

G

G

E

E

G

G

G

G

I

V

N

R

I

P

P

A

D

T

P

P

G

E

Y

F

L

L

K

R

E

A

V

A

R

R

E

E

I

I

C

T

D

Q

E

E

T

K

G

G

A

A

L

L

L

L

I

I

F

L

D
|
D

E

E

V
x
I

Q

Q

T

T

G

G

F

M

G

G

R

R

T

T

G

G

T

K

W

R

F

F

C

A

K

F

E

E

Q

H

F

F

G

G

V

I

E

V

P

P

D

D

I

I

M

L

S

T

M

L

S

A

K
|
K

A

A

I

L

G

G

G

G

G

G

F

V

P

P

M

L

G

G

A

V

I

A

A

V

A

M

H

R

N

E

G

E

I

V

-

A

-

R

N

S

F

M

G

P

R

K

G

G

Q

G

H

H

A

G

S

T

T
|
T

F

F

G

G

G

G

G

N

P

P

L

L

A

A

C

M

A

A

A

A

A

G

L

V

A

A

S

A

V

I

K

R

V

Y

I

L

R

E

E

R

E

T

K

R

L

L

L

W

E

E

R

R

S

A

K

A

E

E

M

L

G

G

A

P

Y

W

F

F

M

M

K

E

K

K

L

L

A

R

G

A

M

I

V

P

R

S

D

P

D

K

V

I

V

R

E

E

V

V

R

R

G

G

K

M

G

G

L

L

M

M

I

V

G

G

V

L

E

E

I

L

K

K

Y

E

P

K

C

A

G

A

K

P

F

Y

V

I

-

A

D

R

F

L

A

E

R

K

E

E

Q

H

G

R

V

V

L

L

V

A

N

L

C

Q

T

A

S

G

D

P

S

T

V

V

L

I

R
|
R

L

F

V

L

P

P

P

P

L

L

V

V

I

I

T

E

K

K

E

E

Q

D

I

L

D

E

T

R

V

V

V

V

D

E

V

A

L

V

active site: S13 (≠ M27), F132 (= F144), D217 (= D230), K246 (= K259), T275 (= T286), R363 (= R373)
binding pyridoxal-5'-phosphate: G105 (= G117), T106 (≠ A118), F132 (= F144), S133 (≠ H145), E184 (= E197), D217 (= D230), I219 (≠ V232), K246 (= K259)

Q9M8M7 Acetylornithine aminotransferase, chloroplastic/mitochondrial; ACOAT; Acetylornithine transaminase; AOTA; Protein HOPW1-1-INTERACTING 1; EC 2.6.1.11 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
45% identity, 95% coverage: 18:394/395 of query aligns to 61:451/457 of Q9M8M7

query
sites
Q9M8M7

V

V

I

I

E

E

K

E

D

E

S

A

K

K

Y

V

V

I

M

V

Q

G

T

T

Y

Y

G

A

R

R

Q

A

P

P

L

V

V

V

L

L

S

S

K

S

G

G

K

K

G

G

A

C

V

K

V

L

Q

F

D

D

I

P

Y

E

G

G

K

K

E

E

Y

Y

I

L

D

D

C

C

V

A

A

S

G

G

I

I

A

A

V

V

N

N

N

A

V

L

G

G

H

H

C

G

H

D

P

P

T

D

V

W

V

L

K

R

A

A

I

V

Q

T

A

E

Q

Q

A

A

E

G

N

V

L

L

I

A

H

H

V

V

S

S

N

N

L

V

Y

Y

Y

Y

T

T

E

I

I

P

Q

Q

A

I

E

E

F

L

A

A

E

K

T

R

L

L

A

V

S

A

I

S

T

S

G

F

M

A

E

D

R

R

V

V

F

F

F

F

C

C

N

N

S

S

G

G

A

T

E

E

S

A

V

N

E

E

A

A

A

A

M

I

K

K

L

F

A

S

R

R

-

K

-

F

-

Q

-

R

-

F

-

T

-

H

-

P

-

E

-

D

-

K

-

E

V

V

A

A

T

T

G

G

K

-

S

-

A

-

F

F

V

I

A

A

A

F

E

T

H

N

S

S

F

F

H

H

G

G

R

R

T

T

I

L

G

G

A

A

L

L

S

A

V

L

T

T

H

S

K

K

S

E

M

Q

Y

Y

R

R

D

T

P

P

F

F

M

-

P

E

P

P

V

I

S

M

S

P

E

G

T

V

T

T

F

F

V

L

P

E

Y

Y

S

G

D

N

A

I

E

Q

A

A

I

A

R

T

Q

D

A

L

I

I

-

R

S

S

E

G

N

K

T

I

A

A

A

A

V

V

I

F

L

V

E

E

P

P

I

I

Q

Q

G

G

E

E

G

G

G

G

I

I

N

Y

I

S

P

A

D

T

P

K

G

E

Y

F

L

L

K

Q

E

S

V

L

R

R

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S

I

A

C

C

D

D

E

A

T

A

G

G

A

S

L

L

L

L

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F

F

D

D

E

E

V

V

Q

Q

T

C

G

G

F

L

G

G

R

R

T

T

G

G

T

L

W

M

F

W

C

A

K

Y

E

E

Q

A

F

F

G

G

V

V

E

T

P

P

D

D

I

I

M

M

S

T

M

V

S

A

K

K

A

P

I

L

G

A

G

G

G

G

F

L

P

P

M

I

G

G

A

A

I

V

A

L

A

V

H

T

N

E

G

K

I

V

-

A

-

E

N

T

F

I

G

N

R

Y

G

G

Q

D

H

H

A

G

S

S

T

T

F

F

G

A

G

G

G

S

P

P

L

L

A

V

C

C

A

S

A

A

A

A

L

I

A

A

S

V

V

M

K

D

V

K

I

V

R

S

E

K

E

P

K

S

L

F

L

L

E

S

R

S

S

V

K

S

E

N

M

K

G

G

A

R

Y

Y

F

F

-

R

-

D

-

L

-

L

M

V

K

K

K

K

L

L

A

G

G

G

M

-

V

-

R

N

D

S

D

H

V

V

V

K

E

E

V

V

R

R

G

G

K

E

G

G

L

L

M

I

I

I

G

G

V

V

E

E

I

L

K

D

Y

V

P

P

C

A

G

S

K

S

F

L

V

V

D

D

F

A

A

C

R

R

E

D

Q

S

G

G

V

L

L

L

V

I

-

L

N

T

C

A

T

G

S

K

D

G

S

N

V

V

L

V

R

R

L

I

V

V

P

P

P

P

L

L

V

V

I

I

T

S

K

E

E

E

Q

E

I

I

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A

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D

E

V

I

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M

E

S

Q

Q

Sites not aligning to the query:

1:41 modified: transit peptide, Chloroplast and mitochondrion
42 modified: N-acetylvaline; in Acetylornithine aminotransferase, chloroplastic

2eh6A Crystal structure of acetylornithine aminotransferase from aquifex aeolicus vf5
44% identity, 94% coverage: 25:394/395 of query aligns to 2:373/375 of 2eh6A

query
sites
2eh6A

Y

Y

V

L

M

M

Q

N

T

N

Y

Y

G

A

R

R

Q

L

P

P

L

V

V

K

L

F

S

V

K

R

G

G

K

K

G

G

A

V

V

Y

V

L

Q

Y

D

D

I

E

Y

E

G

G

K

K

E

E

Y

Y

I

L

D

D

C

F

V

V

A

S

G

G

I

I

A

G

V

V

N

N

N

S

V

L

G

G

H

H

C

A

H

Y

P

P

T

K

V

L

V

T

K

E

A

A

I

L

Q

K

A

E

Q

Q

A

V

E

E

N

K

L

L

I

L

H

H

V

V

S

S

N

N

L

L

Y

Y

Y

E

T

N

E

P

I

W

Q

Q

A

E

E

E

F

L

A

A

E

H

T

K

L

L

A

V

S

K

I

H

T

F

G

W

M

T

E

E

-

G

R

K

V

V

F

F

F

F

C

A

N

N

S

S

G
|
G

A
x
T

E

E

S

S

V

V

E

E

A

A

A

A

M

I

K

K

L

L

A

A

R

R

V

K

-

Y

-

W

-

R

-

D

-

K

A

G

T

K

G

N

K

K

S

W

A

K

F

F

V

I

A

S

A

F

E

E

H

N

S

S

F
|
F

H
|
H

G

G

R

R

T

T

I

Y

G

G

A

S

L

L

S

S

V

A

T

T

H

G

K

Q

S

P

M

K

Y

F

R

H

D

K

P

G

F

F

M

E

P

P

P

L

V

V

S

P

S

G

E

-

T

F

T

S

F

Y

V

A

P

K

Y

L

S

N

D

D

A

I

E

D

A

S

I

V

R

Y

Q

K

A

L

I

L

S

D

E

E

N

E

T

T

A

A

A

G

V

I

I

I

L

I

E
|
E

P

V

I

I

Q

Q

G

G

E

E

G

G

G

G

I

V

N

N

I

E

P

A

D

S

P

E

G

D

Y

F

L

L

K

S

E

K

V

L

R

Q

E

E

I

I

C

C

D

K

E

E

T

K

G

D

A

V

L

L

L

L

I

I

F

I

D
|
D

E

E

V
|
V

Q
|
Q

T

T

G

G

F

I

G

G

R

R

T

T

G

G

T

E

W

F

F

Y

C

A

K

Y

E

Q

Q

H

F

F

G

N

V

L

E

K

P

P

D

D

I

V

M

I

S

A

M

L

S

A

K
|
K

A

G

I

L

G

G

G

G

G

G

F

V

P

P

M

I

G

G

A

A

I

I

A

L

A

A

H

R

N

E

G

E

I

V

-

A

-

Q

N

S

F

F

G

T

R

P

G

G

Q

S

H

H

A

G

S

S

T
|
T

F

F

G

G

G

G

G

N

P

P

L

L

A

A

C

C

A

R

A

A

A

-

L

-

A

G

S

T

V

V

K

V

V

V

I

D

R

E

E

V

E

E

K

K

L

L

L

L

E

P

R

H

S

V

K

R

E

E

M

V

G

G

A

N

Y

Y

F

F

M

K

K

E

K

K

L

L

A

K

G

E

M

L

V

G

R

K

D

G

D

-

V

-

V

-

E

K

V

V

R

K

G

G

K

R

G

G

L

L

M

M

I

L

G

G

V

L

E

E

I

L

K

E

Y

R

P

E

C

C

G

K

K

D

F

Y

V

V

D

L

F

K

A

A

R

L

E

E

Q

K

G

G

V

L

L

L

V

I

N

N

C

C

T

T

S

A

D

G

S

K

V

V

L

L

R
|
R

L

F

V

L

P

P

P

P

L

L

V

I

I

I

T

Q

K

K

E

E

Q

H

I

I

D

D

T

R

V

A

V

I

D

S

V

V

L

L

E

R

Q

E

active site: F127 (= F144), E179 (= E197), D212 (= D230), Q215 (= Q233), K241 (= K259), T270 (= T286), R352 (= R373)
binding pyridoxal-5'-phosphate: G95 (= G117), T96 (≠ A118), F127 (= F144), H128 (= H145), E179 (= E197), D212 (= D230), V214 (= V232), K241 (= K259)

O66442 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Aquifex aeolicus (strain VF5)
44% identity, 94% coverage: 25:394/395 of query aligns to 3:374/376 of O66442

query
sites
O66442

Y

Y

V

L

M

M

Q

N

T

N

Y

Y

G

A

R

R

Q

L

P

P

L

V

V

K

L

F

S

V

K

R

G

G

K

K

G

G

A

V

V

Y

V

L

Q

Y

D

D

I

E

Y

E

G

G

K

K

E

E

Y

Y

I

L

D

D

C

F

V

V

A

S

G

G

I

I

A

G

V

V

N

N

N

S

V

L

G

G

H

H

C

A

H

Y

P

P

T

K

V

L

V

T

K

E

A

A

I

L

Q

K

A

E

Q

Q

A

V

E

E

N

K

L

L

I

L

H

H

V

V

S

S

N

N

L

L

Y

Y

Y

E

T

N

E

P

I

W

Q

Q

A

E

E

E

F

L

A

A

E

H

T

K

L

L

A

V

S

K

I

H

T

F

G

W

M

T

E

E

-

G

R

K

V

V

F

F

F

F

C

A

N

N

S

S

G
|
G

A
x
T

E

E

S

S

V

V

E

E

A

A

A

A

M

I

K

K

L

L

A

A

R

R

V

K

-

Y

-

W

-

R

-

D

-

K

A

G

T

K

G

N

K

K

S

W

A

K

F

F

V

I

A

S

A

F

E

E

H

N

S

S

F

F

H

H

G

G

R

R

T

T

I

Y

G

G

A

S

L

L

S

S

V

A

T

T

H

G

K

Q

S

P

M

K

Y

F

R

H

D

K

P

G

F

F

M

E

P

P

P

L

V

V

S

P

S

G

E

-

T

F

T

S

F

Y

V

A

P

K

Y

L

S

N

D

D

A

I

E

D

A

S

I

V

R

Y

Q

K

A

L

I

L

S

D

E

E

N

E

T

T

A

A

A

G

V

I

I

I

L

I

E

E

P

V

I

I

Q

Q

G

G

E

E

G

G

G

G

I

V

N

N

I

E

P

A

D

S

P

E

G

D

Y

F

L

L

K

S

E

K

V

L

R

Q

E

E

I

I

C

C

D

K

E

E

T

K

G

D

A

V

L

L

L

L

I

I

F

I

D

D

E

E

V

V

Q

Q

T

T

G

G

F

I

G

G

R

R

T

T

G

G

T

E

W

F

F

Y

C

A

K

Y

E

Q

Q

H

F

F

G

N

V

L

E

K

P

P

D

D

I

V

M

I

S

A

M

L

S

A

K
|
K

A

G

I

L

G

G

G

G

G

G

F

V

P

P

M

I

G

G

A

A

I

I

A

L

A

A

H

R

N

E

G

E

I

V

-

A

-

Q

N

S

F

F

G

T

R

P

G

G

Q

S

H

H

A

G

S

S

T
|
T

F

F

G

G

G

G

G

N

P

P

L

L

A

A

C

C

A

R

A

A

A

-

L

-

A

G

S

T

V

V

K

V

V

V

I

D

R

E

E

V

E

E

K

K

L

L

L

L

E

P

R

H

S

V

K

R

E

E

M

V

G

G

A

N

Y

Y

F

F

M

K

K

E

K

K

L

L

A

K

G

E

M

L

V

G

R

K

D

G

D

-

V

-

V

-

E

K

V

V

R

K

G

G

K

R

G

G

L

L

M

M

I

L

G

G

V

L

E

E

I

L

K

E

Y

R

P

E

C

C

G

K

K

D

F

Y

V

V

D

L

F

K

A

A

R

L

E

E

Q

K

G

G

V

L

L

L

V

I

N

N

C

C

T

T

S

A

D

G

S

K

V

V

L

L

R

R

L

F

V

L

P

P

P

P

L

L

V

I

I

I

T

Q

K

K

E

E

Q

H

I

I

D

D

T

R

V

A

V

I

D

S

V

V

L

L

E

R

Q

E

GT 96:97 (≠ GA 117:118) binding pyridoxal 5'-phosphate
K242 (= K259) modified: N6-(pyridoxal phosphate)lysine
T271 (= T286) binding pyridoxal 5'-phosphate

A0QYS9 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
45% identity, 96% coverage: 12:392/395 of query aligns to 4:379/390 of A0QYS9

query
sites
A0QYS9

Q

Q

A

S

R

R

Y

W

D

E

S

A

V

V

I

M

E

-

K

-

D

-

S

-

K

-

Y

-

V

-

M

M

Q

N

T

N

Y

Y

G

G

R

T

Q

P

P

P

L

L

V

S

L

L

S

V

K

S

G

G

K

E

G

G

A

A

V

V

V

V

Q

T

D

D

I

A

Y

D

G

G

K

R

E

E

Y

Y

I

L

D

D

C

L

V

L

A

G

G

G

I

I

A

A

V

V

N

N

N

L

V

L

G

G

H

H

C

R

H

H

P

P

T

A

V

V

V

I

K

E

A

A

I

V

Q

T

A

T

Q

Q

A

L

E

D

N

T

L

L

I

G

H

H

V

T

S

S

N

N

L

L

Y

Y

Y

A

T

T

E

E

I

P

Q

G

A

I

E

A

F

L

A

A

E

E

T

A

L

L

A

V

S

G

I

Q

T

L

G

G

M

T

E

Q

-

A

R

R

V

V

F

F

F

F

C

C

N

N

S

S

G

G

A

T

E

E

S

A

V

N

E

E

A

V

A

A

M

F

K

K

L

I

A

T

R

R

V

L

A

-

T

T

G

G

K

K

S

T

A

K

F

I

V

V

A

A

A

A

E

E

H

G

S

A

F

F

H

H

G

G

R

R

T

T

I

M

G

G

A

S

L

L

S

A

V

L

T

T

H

G

K

Q

S

P

M

S

Y

K

R

Q

D

A

P

P

F

F

M

-

P

E

P

P

V

L

S

P

S

G

E

N

T

V

T

M

F

H

V

V

P

P

Y

Y

S

G

D

D

A

V

E

A

A

A

I

L

R

E

Q

A

A

A

I

V

S

D

E

D

N

Q

T

T

A

A

A

A

V

V

I

F

L

L

E

E

P

P

I

I

Q

M

G

G

E

E

G

G

G

G

I

V

N

V

I

V

P

P

D

P

P

A

G

G

Y

Y

L

L

K

V

E

A

V

A

R

R

E

E

I

I

C

T

D

S

E

K

T

H

G

G

A

A

L

L

L

L

I

V

F

L

D

D

E

E

V

V

Q

Q

T

T

G

G

F

V

G

G

R

R

T

T

G

G

T

A

W

F

F

F

C

A

K

H

E

Q

Q

H

F

D

G

G

V

I

E

V

P

P

D

D

I

V

M

V

S

T

M

M

S

A

K

K

A

G

I

L

G

G

G

G

G

G

F

L

P

P

M

I

G

G

A

A

I

C

A

L

A

A

-

V

-

G

H

A

N

T

G

G

I

D

N

L

F

L

G

T

R

P

G

G

Q

L

H

H

A

G

S

S

T

T

F

F

G

G

G

G

G

N

P

P

L

V

A

C

C

T

A

A

A

A

A

G

L

L

A

A

S

V

V

L

K

K

V

T

I

L

R

A

E

A

E

E

K

D

L

L

L

V

E

A

R

R

S

A

K

G

E

V

M

L

G

G

A
x
K

Y

T

F

L

M

S

K

H

K

G

L

I

A

E

G

E

M

L

V

G

R

H

D

P

D

L

V

V

V

D

E

K

V

V

R

R

G

G

K

K

G

G

L

L

M

L

I

Q

G

G

V

I

E

V

I

L

K

T

Y

V

P

P

C

S

G

A

K

K

F

A

V

V

D

E

F

T

-

A

A

A

R

R

E

D

Q

A

G

G

V

F

L

L

V

V

N

N

C

A

T

A

S

A

D

P

S

E

V

V

L

V

R

R

L

L

V

A

P

P

P

P

L

L

V

I

I

I

T

T

K

E

E

G

Q

Q

I

I

D

E

T

A

V

F

V

I

D

T

V

A

L

L

K304 (≠ A318) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

8ht4B Crystal structure of acetylornithine aminotransferase complex with plp from corynebacterium glutamicum (see paper)
44% identity, 96% coverage: 17:394/395 of query aligns to 1:387/390 of 8ht4B

query
sites
8ht4B

S

S

V

T

I

L

E

E

K

T

D

W

S

P

K

Q

Y

V

V

I

M

I

Q

N

T

T

Y

Y

G

G

R

T

Q

P

P

P

L

V

V

E

L

L

S

V

K

S

G

G

K

K

G

G

A

A

V

T

V

V

Q

T

D

D

I

D

Y

Q

G

G

K

N

E

V

Y

Y

I

I

D

D

C

L

V

L

A

A

G

G

I

I

A

A

V

V

N

N

N

A

V

L

G

G

H

H

C

A

H

H

P

P

T

A

V

I

V

I

K

E

A

A

I

V

Q

T

A

N

Q

Q

A

I

E

G

N

Q

L

L

I

G

H

H

V

V

S

S

N

N

L

L

Y

F

Y

A

T

S

E

R

I

P

Q

V

A

V

E

E

F

V

A

A

E

E

T

E

L

L

-

I

-

K

-

R

-

F

-

S

-

L

-

D

-

D

A

A

S

T

I

L

T

A

G

A

M

Q

E

T

R

R

V

V

F

F

F

F

C

C

N

N

S
|
S

G
|
G

A
|
A

E

E

S

A

V

N

E

E

A

A

A

A

M

F

K

K

L

I

A

A

R

R

V

L

A

-

T

T

G

G

K

R

S

S

A

R

F

I

V

L

A

A

A

A

E

V

H

H

S

G

F
|
F

H
|
H

G

G

R

R

T

T

I

M

G

G

A

S

L

L

S

A

V

L

T

T

H

G

K

Q

S

P

M

D

Y

K

R

R

D

E

P

A

F

F

M

L

P

P

P

-

V

M

S

P

S

S

E

G

T

V

T

E

F

F

V

Y

P

P

Y

Y

S

G

D

D

A

T

E

D

A

Y

I

L

R

R

Q

K

A

M

I

V

S

E

E

T

N

N

-

P

-

T

-

D

T

V

A

A

A

A

V

I

I

F

L

L

E
|
E

P

P

I

I

Q

Q

G

G

E

E

G

T

G

G

I

V

N

-

I

V

P

P

D

A

P

P

-

E

G

G

Y

F

L

L

K

K

E

A

V

V

R

R

E

E

I

L

C

C

D

D

E

E

T

Y

G

G

A

I

L

L

L

M

I

I

F

T

D
|
D

E

E

V
|
V

Q
|
Q

T

T

G

G

F

V

G

G

R

R

T

T

G

G

T

D

W

F

F

F

C

A

K

H

E

Q

Q

H

F

D

G

G

V

V

E

V

P

P

D

D

I

V

M

V

S

T

M

M

S

A

K
|
K

A

G

I

L

G

G

G

G

G

G

F

L

P

P

M

I

G

G

A

A

I

C

A

L

A

A

H

T

N

-

G

-

I

-

N

-

F

-

G

G

R

R

-

A

-

A

-

E

-

L

-

M

-

T

-

P

G

G

Q

K

H

H

A

G

S

T

T

T

F

F

G

G

G

G

G

N

P

P

L

V

A

A

C

C

A

A

A

A

A

A

L

K

A

A

S

V

V

L

K

S

V

V

I

V

R

D

E

D

E

A

K

F

L

C

L

A

E

E

R

V

S

A

K

R

E

K

M

-

G

G

A

E

Y

L

F

F

M

K

K

E

K

L

L

L

A

A

G

K

M

V

V

-

R

-

D

D

D

G

V

V

V

V

E

D

V

V

R

R

G

G

K

R

G

G

L

L

M

M

I

L

G

G

V

V

E

V

I

L

K

E

Y

R

P

D

C

V

G

A

K

K

-

Q

F

A

V

V

D

L

F

D

A

G

R

F

E

K

Q

H

G

G

V

V

L

I

V

L

N

N

C

A

T

P

S

A

D

D

S

N

V

I

L

I

R

R

L

L

V

T

P

P

P

P

L

L

V

V

I

I

T

T

K

D

E

E

Q

E

I

I

D

A

T

D

V

A

V

V

D

K

V

A

L

I

E

A

Q

E

binding pyridoxal-5'-phosphate: S108 (= S116), G109 (= G117), A110 (= A118), F135 (= F144), H136 (= H145), E190 (= E197), D223 (= D230), V225 (= V232), Q226 (= Q233), K252 (= K259)

P9WPZ7 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
43% identity, 94% coverage: 20:392/395 of query aligns to 13:389/400 of P9WPZ7

query
sites
P9WPZ7

E

Q

K

R

D

W

S

Q

K

A

Y

V

V

M

M

M

Q

N

T

N

Y

Y

G

G

R

T

Q

P

P

P

L

I

V

A

L

L

S

A

K

S

G

G

K

D

G

G

A

A

V

V

V

V

Q

T

D

D

I

V

Y

D

G

G

K

R

E

T

Y

Y

I

I

D

D

C

L

V

L

A

G

G

G

I

I

A

A

V

V

N

N

N

V

V

L

G

G

H

H

C

R

H

H

P

P

T

A

V

V

V

I

K

E

A

A

I

V

Q

T

A

R

Q

Q

A

M

E

S

N

T

L

L

I

G

H

H

V

T

S

S

N

N

L

L

Y

Y

Y

A

T

T

E

E

I

P

Q

G

A

I

E

A

F

L

A

A

E

E

T

E

L

L

A

V

S

A

I

L

T

L

G

G

M

A

E

D

-

Q

-

R

-

T

R

R

V

V

F

F

F

F

C

C

N

N

S

S

G

G

A

A

E

E

S

A

V

N

E

E

A

A

A

A

M

F

K

K

L

L

A

S

R

R

V

L

A

-

T

T

G

G

K

R

S

T

A

K

F

L

V

V

A

A

A

A

E

H

H

D

S

A

F

F

H

H

G

G

R

R

T

T

I

M

G

G

A

S

L

L

S

A

V

L

T

T

H

G

K

Q

S

P

M

A

Y

K

R

Q

D

T

P

P

F

F

M

A

P

P

P

-

V

L

S

P

S

G

E

D

T

V

T

T

F

H

V

V

P

G

Y

Y

S

G

D

D

A

V

E

D

A

A

I

L

R

A

Q

A

A

A

I

V

S

D

E

D

N

H

T

T

A

A

A

A

V

V

I

F

L

L

E

E

P

P

I

I

Q

M

G

G

E

E

G

S

G

G

I

V

N

V

I

V

P

P

D

P

P

A

G

G

Y

Y

L

L

K

A

E

A

V

A

R

R

E

D

I

I

C

T

D

A

E

R

T

R

G

G

A

A

L

L

L

L

I

V

F

L

D

D

E

E

V

V

Q

Q

T

T

G

G

F

M

G

G

R

R

T

T

G

G

T

A

W

F

F

F

C

A

K

H

E

Q

Q

H

F

D

G

G

V

I

E

T

P

P

D

D

I

V

M

V

S

T

M

L

S

A

K

K

A

G

I

L

G

G

G

G

G

G

F

L

P

P

M

I

G

G

A

A

I

C

A

L

A

A

H

V

N

G

G

P

I

A

N

A

-

E

-

L

F

L

G

T

R

P

G

G

Q

L

H

H

A

G

S

S

T

T

F

F

G

G

G

G

G

N

P

P

L

V

A

C

C

A

A

A

A

A

A

A

L

L

A

A

S

V

V

L

K

R

V

V

I

L

R

A

E

S

E

D

K

G

L

L

L

V

E

R

R

R

S

A

K

E

E

V

M

L

G

G

A
x
K

Y

S

F

L

M

R

K

H

K

G

L

I

A

E

G

A

M

L

V

G

R

H

D

P

D

L

V

I

V

D

E

H

V

V

R

R

G

G

K

R

G

G

L

L

M

L

I

L

G

G

V

I

E

A

I

L

K

T

Y

A

P

P

C

H

G

A

K

K

F

D

V

A

D

E

-

A

F

T

A

A

R

R

E

D

Q

A

G

G

V

Y

L

L

V

V

N

N

C

A

T

A

S

A

D

P

S

D

V

V

L

I

R

R

L

L

V

A

P

P

P

P

L

L

V

I

I

I

T

A

K

E

E

A

Q

Q

I

L

D

D

T

G

V

F

V

V

D

A

V

A

L

L

K314 (≠ A318) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylthreonine; partial

7nncC Crystal structure of mycobacterium tuberculosis argd with prosthetic group pyridoxal-5'-phosphate and 6-methoxyquinoline-3-carboxylic acid
43% identity, 94% coverage: 20:392/395 of query aligns to 7:383/391 of 7nncC

query
sites
7nncC

E

Q

K

R

D

W

S

Q

K

A

Y

V

V

M

M

M

Q

N

T

N

Y

Y

G

G

R

T

Q

P

P

P

L

I

V

A

L

L

S

A

K

S

G

G

K

D

G

G

A

A

V

V

V

V

Q

T

D

D

I

V

Y

D

G

G

K

R

E

T

Y

Y

I

I

D

D

C

L

V

L

A

G

G

G

I

I

A

A

V

V

N

N

N

V

V

L

G

G

H

H

C

R

H

H

P

P

T

A

V

V

V

I

K

E

A

A

I

V

Q

T

A

R

Q

Q

A

M

E

S

N

T

L

L

I

G

H

H

V

T

S

S

N
|
N

L
|
L

Y

Y

Y

A

T

T

E

E

I

P

Q

G

A

I

E

A

F

L

A

A

E

E

T

E

L

L

A

V

S

A

I

L

T

L

G

G

M

A

E

D

-

Q

-

R

-

T

R

R

V

V

F

F

F

F

C

C

N

N

S

S

G

G

A

A

E

E

S

A

V

N

E

E

A

A

A

A

M

F

K

K

L

L

A

S

R

R

V

L

A

-

T

T

G

G

K

R

S

T

A

K

F

L

V

V

A

A

A

A

E

H

H

D

S

A

F
|
F

H

H

G

G

R

R

T

T

I

M

G

G

A

S

L

L

S

A

V

L

T

T

H

G

K

Q

S

P

M

A

Y

K

R

Q

D

T

P

P

F

F

M

A

P

P

P

-

V

L

S

P

S

G

E

D

T

V

T

T

F

H

V

V

P

G

Y

Y

S

G

D

D

A

V

E

D

A

A

I

L

R

A

Q

A

A

A

I

V

S

D

E

D

N

H

T

T

A

A

A

A

V

V

I

F

L

L

E

E

P

P

I

I

Q

M

G

G

E

E

G

S

G

G

I

V

N

V

I

V

P

P

D

P

P

A

G

G

Y

Y

L

L

K

A

E

A

V

A

R

R

E

D

I

I

C

T

D

A

E

R

T

R

G

G

A

A

L

L

L

L

I

V

F

L

D
|
D

E

E

V

V

Q

Q

T

T

G

G

F

M

G

G

R

R

T

T

G

G

T

A

W

F

F

F

C

A

K

H

E

Q

Q

H

F

D

G

G

V

I

E

T

P

P

D

D

I

V

M

V

S

T

M

L

S

A

K
|
K

A

G

I

L

G

G

G

G

G

G

F

L

P

P

M

I

G

G

A

A

I

C

A

L

A

A

H

V

N

G

G

P

I

A

N

A

-

E

-

L

F

L

G

T

R

P

G

G

Q

L

H

H

A

G

S

S

T

T

F

F

G

G

G

G

G

N

P

P

L

V

A

C

C

A

A

A

A

A

A

A

L

L

A

A

S

V

V

L

K

R

V

V

I

L

R

A

E

S

E

D

K

G

L

L

L

V

E

R

R

R

S

A

K

E

E

V

M

L

G

G

A

K

Y

S

F

L

M

R

K

H

K

G

L

I

A

E

G

A

M

L

V

G

R

H

D

P

D

L

V

I

V

D

E

H

V

V

R

R

G

G

K

R

G

G

L

L

M

L

I

L

G

G

V

I

E

A

I

L

K

T

Y

A

P

P

C

H

G

A

K

K

F

D

V

A

D

E

-

A

F

T

A

A

R

R

E

D

Q

A

G

G

V

Y

L

L

V

V

N

N

C

A

T

A

S

A

D

P

S

D

V

V

L

I

R

R

L

L

V

A

P

P

P

P

L

L

V

I

I

I

T

A

K

E

E

A

Q

Q

I

L

D

D

T

G

V

F

V

V

D

A

V

A

L

L

active site: F133 (= F144), D218 (= D230), K247 (= K259)
binding 6-methoxyquinoline-3-carboxylic acid: N75 (= N88), L76 (= L89)

7nn4A Crystal structure of mycobacterium tuberculosis argd with prosthetic group pyridoxal 5'-phosphate and 3-hydroxy-2-naphthoic acid.
43% identity, 94% coverage: 20:392/395 of query aligns to 7:383/391 of 7nn4A

query
sites
7nn4A

E

Q

K

R

D

W

S

Q

K

A

Y

V

V

M

M

M

Q

N

T

N

Y
|
Y

G

G

R

T

Q

P

P

P

L

I

V

A

L

L

S

A

K

S

G

G

K

D

G

G

A

A

V

V

V

V

Q

T

D

D

I

V

Y

D

G

G

K

R

E

T

Y

Y

I

I

D

D

C

L

V

L

A

G

G

G

I

I

A

A

V

V

N

N

N

V

V

L

G

G

H

H

C

R

H

H

P

P

T

A

V

V

V

I

K

E

A

A

I

V

Q

T

A

R

Q

Q

A

M

E

S

N

T

L

L

I

G

H

H

V

T

S

S

N

N

L
|
L

Y

Y

Y

A

T

T

E

E

I

P

Q

G

A

I

E

A

F

L

A

A

E

E

T

E

L

L

A

V

S

A

I

L

T

L

G

G

M

A

E

D

-

Q

-

R

-

T

R

R

V

V

F

F

F

F

C

C

N

N

S

S

G

G

A

A

E

E

S

A

V

N

E

E

A

A

A

A

M

F

K

K

L

L

A

S

R

R

V

L

A

-

T

T

G

G

K

R

S

T

A

K

F

L

V

V

A

A

A

A

E

H

H

D

S

A

F
|
F

H

H

G

G

R
|
R

T

T

I

M

G

G

A

S

L

L

S

A

V

L

T

T

H

G

K

Q

S

P

M

A

Y

K

R

Q

D

T

P

P

F

F

M

A

P

P

P

-

V

L

S

P

S

G

E

D

T

V

T

T

F

H

V

V

P

G

Y

Y

S

G

D

D

A

V

E

D

A

A

I

L

R

A

Q

A

A

A

I

V

S

D

E

D

N

H

T

T

A

A

A

A

V

V

I

F

L

L

E

E

P

P

I

I

Q

M

G

G

E

E

G

S

G

G

I

V

N

V

I

V

P

P

D

P

P

A

G

G

Y

Y

L

L

K

A

E

A

V

A

R

R

E

D

I

I

C

T

D

A

E

R

T

R

G

G

A

A

L

L

L

L

I

V

F

L

D
|
D

E

E

V

V

Q

Q

T

T

G

G

F

M

G

G

R

R

T

T

G

G

T

A

W

F

F

F

C

A

K

H

E

Q

Q

H

F

D

G

G

V

I

E

T

P

P

D

D

I

V

M

V

S

T

M

L

S

A

K
|
K

A

G

I

L

G

G

G

G

G

G

F

L

P

P

M

I

G

G

A

A

I

C

A

L

A

A

H

V

N

G

G

P

I

A

N

A

-

E

-

L

F

L

G

T

R

P

G

G

Q

L

H

H

A
x
G

S

S

T

T

F

F

G

G

G

G

G

N

P

P

L

V

A

C

C

A

A

A

A

A

A

A

L

L

A

A

S

V

V

L

K

R

V

V

I

L

R

A

E

S

E

D

K

G

L

L

L

V

E

R

R

R

S

A

K

E

E

V

M

L

G

G

A

K

Y

S

F

L

M

R

K

H

K

G

L

I

A

E

G

A

M

L

V

G

R

H

D

P

D

L

V

I

V

D

E

H

V

V

R

R

G

G

K

R

G

G

L

L

M

L

I

L

G

G

V

I

E

A

I

L

K

T

Y

A

P

P

C

H

G

A

K

K

F

D

V

A

D

E

-

A

F

T

A

A

R

R

E

D

Q

A

G

G

V

Y

L

L

V

V

N

N

C

A

T

A

S

A

D

P

S

D

V

V

L

I

R

R

L

L

V

A

P

P

P

P

L

L

V

I

I

I

T

A

K

E

E

A

Q

Q

I

L

D

D

T

G

V

F

V

V

D

A

V

A

L

L

active site: F133 (= F144), D218 (= D230), K247 (= K259)
binding 3-hydroxynaphthalene-2-carboxylic acid: Y17 (= Y30), L76 (= L89), <