SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_048080275.1 NCBI__GCF_000745485.1:WP_048080275.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1wqaA Crystal structure of pyrococcus horikoshii phosphomannomutase/phosphoglucomutase complexed with mg2+
43% identity, 99% coverage: 7:451/451 of query aligns to 3:454/455 of 1wqaA

query
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1wqaA

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active site: R11 (= R15), S101 (= S102), H102 (= H103), K111 (= K112), D243 (= D241), D245 (= D243), D247 (= D245), R248 (= R246), G330 (≠ T323), R340 (≠ P333)
binding magnesium ion: S101 (= S102), D243 (= D241), D245 (= D243), D247 (= D245)

7omlA Bacillus subtilis phosphoglucomutase glmm (metal bound) (see paper)
32% identity, 95% coverage: 7:435/451 of query aligns to 2:428/445 of 7omlA

query
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7omlA

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binding magnesium ion: S99 (= S102), D239 (= D241), D241 (= D243), D243 (= D245)

7ojrA Bacillus subtilis phosphoglucomutase glmm (phosphate bound) (see paper)
32% identity, 95% coverage: 7:435/451 of query aligns to 2:428/445 of 7ojrA

query
sites
7ojrA

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P

P

T

Q

V

L

R

L

D

V

K

N

I

V

E

R

C

V

E

T

N

D

I

K

Q

Y

K

K

T

V

L

E

I

E

M

N

E

E

K

K

V

V

K

K

E

A

E

-

L

-

P

-

D

-

Y

-

F

-

E

-

D

-

V

-

N

-

D

-

V

-

N

-

F

V

I

I

D

S

G

E

V

V

R

E

I

K

S

E

M

M

K

N

D

G

G

D

S

G

W

R

V

I

L

L

I

V

R
|
R

P

P

S
|
S

G

G

T
|
T

E

E

S

P

Y

L

I

V

R
|
R

I

V

T

M

L

A

E

E

G

A

R

K

N

T

V

K

E

E

I

L

binding phosphate ion: R409 (= R416), S411 (= S418), T413 (= T420), R418 (= R425)

3i3wA Structure of a phosphoglucosamine mutase from francisella tularensis
32% identity, 98% coverage: 7:447/451 of query aligns to 1:432/441 of 3i3wA

query
sites
3i3wA

R

K

L

Y

F

F

G

G

T

T

S

D

G

G

I

I

R
|
R

G

G

K

E

I

V

G

A

-

N

S

S

E

T

I

I

T

T

L

V

N

E

L

F

I

T

T

Q

D

K

V

L

G

G

M

N

A

A

A

V

A

G

T

S

Y

L

V

I

G

N

G

Q

K

K

G

N

-

Y

-

P

R

K

K

F

A

V

V

I

I

V

G

G

Y

Q

D

D

T

T

R

R

T

S

S

S

N

G

K

G

M

F

V

L

E

K

N

F

A

A

I

L

I

V

A

S

G

G

I

L

L

N

Q

A

C

A

G

G

C

I

D

D

V

V

I

L

R

D

L

L

G

G

M

V

V

V

P

P

T

T

P

P

L

V

V

V

G

A

Y

F

A

M

A

T

M

V

K

K

L

H

N

R

A

A

D

A

I

A

G

G

I

F

M

V

I

I

T

T

A

A

S
|
S

H
|
H

N

N

P

K

S

F

Q

T

Y

D

N

N

G

G

I

I

K
|
K

L

L

W

F

N

S

P

S

K

N

G

G

M

F

A

K

Y

L

T

D

Q

D

D

A

Q

L

E

E

R

E

T

E

I

V

E

E

K

D

I

M

V

I

H

D

E

G

K

D

T

F

F

I

S

Y

K

Q

V

P

S

Q

W

F

E

K

H

-

I

F

G

G

D

S

I

Y

K

K

D

I

N

L

S

A

S

N

I

A

I

I

S

D

G

E

Y

Y

M

I

D

E

D

S

L

I

L

Y

N

S

N

R

-

F

-

A

-

K

-

F

V

V

D

N

I

Y

K

K

G

G

G

-

L

-

K

K

V

V

V

V

V

V

D

D

C

C

A

A

N

H

G

G

A

A

G

A

S

S

F

H

V

N

S

F

P

E

T

A

V

L

L

L

R

D

K

K

A

F

G

G

C

I

E

N

V

Y

L

V

T

S

L

I

N

A

S

S

Q

N

P

P

D

D

G

G

F

L

F

-

P

-

G

-

R

N

M

I

P

N

E

V

P

G

S

C

E

G

A

A

N

T

-

C

L

V

S

S

E

N

L

I

M

K

K

K

V

A

V

V

K

K

A

E

T

Q

G

K

A

A

D

D

I

L

G

G

I

I

A

S

H

L

D
|
D

G

G

D
|
D

A

A

D
|
D

R
|
R

M

I

V

I

A

I

I

V

D

D

D

E

E

N

G

G

K

Q

M

E

A

I

D

D

F

G

D

D

-

G

-

I

-

L

K

N

L

I

L

L

A

A

L

Q

V

Y

S

S

S

D

K

I

I

C

G

G

G

G

K

T

-

N

-

G

I

I

V

V

T

G

T

T

V

Q

D

M

A

T

S

N

F

M

C

S

V

Y

D

E

K

N

C

H

M

Y

K

R

E

A

S

N

G

K

G

I

E

P

V

F

V

I

R

R

T

S

K

K

V

V

G

G

D

D

V

R

H

Y

V

V

A

L

E

E

A

D

I

L

V

V

E

K

C

Y

N

G

A

Y

A

K

F

I

G

G

G

G

E

E

P

S

S

S

G

G

T
x
H

W

V

L

I

H

N

P

L

D

N

F

F

C

G

M

T

C

T

P

G

D

D

G

G

I

L

L

F

S

T

A

A

L

I

K

Q

V

L

I

L

E

A

I

I

V

F

E

S

K

Q

Y

-

G

-

P

-

L

-

S

-

K

-

L

-

L

-

N

-

E

-

I

-

P

A

S

D

Y

K

P

P

T

V

V

S

R

E

D

F

K

K

I

L

E

Q

C

G

E

E

N

L

I

M

Q

Q

K

Q

T

T

L

L

I

I

-

N

-

V

-

P

-

L

M

T

E

K

K

K

V

V

-

A

K

R

E

E

E

D

L

L

P

Q

D

K

Y

V

F

A

E

S

D

D

V

V

N

N

D

D

V

V

N

E

F

K

I

R

D

L

G

G

V

N

R

R

I

-

S

-

M

-

K

-

D

-

G

-

S

G

W

R

V

V

L

L

I

L

R

R

P

P

S

S

G

G

T

T

E

E

S

P

Y

V

I

L

R

R

I

V

T

M

L

V

E

E

G

A

R

D

N

D

V

-

E

-

I

-

A

-

Q

K

S

S

I

L

R

A

T

T

K

N

S

E

R

A

E

E

F

Y

I

L

active site: R9 (= R15), S99 (= S102), H100 (= H103), K109 (= K112), D237 (= D241), D239 (= D243), D241 (= D245), R242 (= R246), H324 (≠ T323)
binding zinc ion: S99 (= S102), D237 (= D241), D239 (= D243), D241 (= D245)

2h5aX Complex of the enzyme pmm/pgm with xylose 1-phosphate (see paper)
29% identity, 98% coverage: 8:450/451 of query aligns to 5:447/455 of 2h5aX

query
sites
2h5aX

L

I

F

F

G

R

T

A

S
x
Y

G

D

I

I

R

R

G

G

K

V

I

V

G

G

S

D

E

T

I

L

T

T

L

A

N

E

L

T

I

A

T

Y

D

W

V

I

G

G

M

R

A

A

A

I

A

G

T

S

Y

E

V

S

G

L

G

A

K

R

G

G

R

E

K

P

A

C

V

V

-

A

I

V

G

G

Y

R

D

D

T

G

R

R

T

L

S

S

N

G

K

P

M

E

V

L

E

V

N

K

A

Q

I

L

I

I

A

Q

G

G

I

L

L

V

Q

D

C

C

G

G

C

C

D

Q

V

V

I

S

R

D

L

V

G

G

M

M

V

V

P

P

T

T

P

P

L

V

V

L

G

Y

Y

Y

A

A

A

A

M

N

K

V

L

L

N

E

A

G

D

K

I

S

G

G

I

V

M

M

I

L

T

T

A

G

S
|
S

H
|
H

N

N

P

P

S

P

Q

D

Y

Y

N

N

G

G

I

F

K

K

L

I

W

V

N

V

P

-

K

A

G

G

M

E

A

T

Y

L

T

A

Q

N

D

E

Q

Q

E

I

R

Q

T

A

I

L

E

R

K

E

I

R

V

I

H

E

E

K

K

N

T

D

F

L

S

A

K

S

V

-

S

-

W

-

E

-

H

G

I

V

G

G

D

S

I

V

K

-

D

E

N

Q

S

V

S

D

I

I

I

L

S

P

G

R

Y

Y

M

F

D

K

D

Q

L

I

L

R

N

D

N

D

V

I

D

A

I

M

K

A

G

K

G

P

L

M

K

K

V

V

V

V

V

V

D

D

C

C

A

G

N

N

G

G

A

V

G

A

S

G

F

V

V

I

S

A

P

P

T

Q

V

L

L

I

R

E

K

A

A

L

G

G

C

C

E

S

V

V

L

I

T

P

L

L

N

Y

S

C

Q

E

P

V

D

D

G

G

F

N

F

F

P

P

G

N

R

H

M

H

P

P

E

D

P

P

S

G

E

K

A

P

-

E

N

N

L

L

S

K

E

D

L

L

M

I

K

A

V

K

V

V

K

K

A

A

T

E

G

N

A

A

D

D

I

L

G

G

I

L

A

A

H

F

D
|
D

G

G

D
|
D

A

G

D
|
D

R
|
R

M

V

V

G

A

V

I

V

D

T

D

N

E

T

G

G

K

T

M

I

A

I

D

Y

F

P

D

D

K

R

L

L

L

L

A

M

L

L

-

F

-

A

-

K

-

D

-

V

V

V

S

S

S

R

K

N

I

P

G

G

G

A

K

D

I

I

V

I

T

F

T

D

V

V

D

K

A

C

S

T

F

R

C

R

V

L

D

I

K

A

C

L

M

I

K

S

E

G

S

Y

G

G

G

G

E

R

V

P

V

V

R

M

T

W

K

K

V
x
T

G
|
G

D
x
H

V

S

H

L

V

I

A

K

E

K

A

K

I

M

V

K

E

E

C

T

N

G

A

A

A

L

F

L

G

A

G

G

E
|
E

P

M

S
|
S

G

G

-
x
H

T

V

W

F

L

F

H

K

P

E

D

R

F

W

C

F

M

G

C

F

P
x
D

D

D

G

G

I

I

L

Y

S

S

A

A

L

A

K

R

V

L

I

L

E

E

I

I

V

L

E

S

K

Q

Y

D

G

Q

P

R

L

D

S

S

K

E

-

H

L

V

L

F

N

S

E

A

I

F

P

P

S

S

-

D

Y

I

P

S

T

T

V

P

R

E

D

I

K

N

I

I

E

T

C

V

E

T

N

E

I

D

Q

S

K

K

T

F

L

A

I

I

M

I

E

E

K

A

V

L

K

Q

E

R

E

-

L

-

P

-

D

D

Y

A

F

Q

E

W

D

G

V

E

N

G

D

N

V

I

N

T

F

T

I

L

D

D

G

G

V

V

R

R

I

V

S

D

M

Y

K

P

D

K

G

G

S

-

W

W

V

G

L

L

I

V

R
|
R

P

A

S
|
S

G
x
N

T
|
T

E

T

S

P

Y

V

I

L

R

V

I

L

T

R

L

F

E

E

G

A

R

D

N

T

V

E

E

E

I

E

A

L

Q

E

S

R

I

I

R

K

T

T

K

V

S

F

R

R

E

N

F

Q

I

L

E

K

G

A

I

V

active site: H101 (= H103), D234 (= D241), D236 (= D243), D238 (= D245), R239 (= R246), D332 (≠ P333)
binding 1-O-phosphono-alpha-D-xylopyranose: Y9 (≠ S12), T298 (≠ V300), G299 (= G301), H300 (≠ D302), E317 (= E319), S319 (= S321), H321 (vs. gap), R413 (= R416), S415 (= S418), N416 (≠ G419), T417 (= T420)
binding zinc ion: S100 (= S102), D234 (= D241), D236 (= D243), D238 (= D245)

2h4lX Complex of pmm/pgm with ribose 1-phosphate (see paper)
29% identity, 98% coverage: 8:450/451 of query aligns to 5:447/455 of 2h4lX

query
sites
2h4lX

L

I

F

F

G

R

T

A

S
x
Y

G

D

I

I

R
|
R

G

G

K

V

I

V

G

G

S

D

E

T

I

L

T

T

L

A

N

E

L

T

I

A

T

Y

D

W

V

I

G

G

M

R

A

A

A

I

A

G

T

S

Y

E

V

S

G

L

G

A

K

R

G

G

R

E

K

P

A

C

V

V

-

A

I

V

G

G

Y

R

D

D

T

G

R

R

T

L

S

S

N

G

K

P

M

E

V

L

E

V

N

K

A

Q

I

L

I

I

A

Q

G

G

I

L

L

V

Q

D

C

C

G

G

C

C

D

Q

V

V

I

S

R

D

L

V

G

G

M

M

V

V

P

P

T

T

P

P

L

V

V

L

G

Y

Y

Y

A

A

A

A

M

N

K

V

L

L

N

E

A

G

D

K

I

S

G

G

I

V

M

M

I

L

T

T

A

G

S
|
S

H
|
H

N

N

P

P

S

P

Q

D

Y

Y

N

N

G

G

I

F

K

K

L

I

W

V

N

V

P

-

K

A

G

G

M

E

A

T

Y

L

T

A

Q

N

D

E

Q

Q

E

I

R

Q

T

A

I

L

E

R

K

E

I

R

V

I

H

E

E

K

K

N

T

D

F

L

S

A

K

S

V

-

S

-

W

-

E

-

H

G

I

V

G

G

D

S

I

V

K

-

D

E

N

Q

S

V

S

D

I

I

I

L

S

P

G

R

Y

Y

M

F

D

K

D

Q

L

I

L

R

N

D

N

D

V

I

D

A

I

M

K

A

G

K

G

P

L

M

K

K

V

V

V

V

V

V

D

D

C

C

A

G

N

N

G

G

A

V

G

A

S

G

F

V

V

I

S

A

P

P

T

Q

V

L

L

I

R

E

K

A

A

L

G

G

C

C

E

S

V

V

L

I

T

P

L

L

N

Y

S

C

Q

E

P

V

D

D

G

G

F

N

F

F

P

P

G

N

R

H

M

H

P

P

E

D

P

P

S

G

E

K

A

P

-

E

N

N

L

L

S

K

E

D

L

L

M

I

K

A

V

K

V

V

K

K

A

A

T

E

G

N

A

A

D

D

I

L

G

G

I

L

A

A

H

F

D
|
D

G

G

D
|
D

A

G

D
|
D

R
|
R

M

V

V

G

A

V

I

V

D

T

D

N

E

T

G

G

K

T

M

I

A

I

D

Y

F

P

D

D

K

R

L

L

L

L

A

M

L

L

-

F

-

A

-

K

-

D

-

V

V

V

S

S

S

R

K

N

I

P

G

G

G

A

K

D

I

I

V

I

T

F

T

D

V

V

D

K

A

C

S

T

F

R

C

R

V

L

D

I

K

A

C

L

M

I

K

S

E

G

S

Y

G

G

G

G

E

R

V

P

V

V

R

M

T

W

K

K

V
x
T

G

G

D

H

V

S

H

L

V

I

A

K

E

K

A

K

I

M

V

K

E

E

C

T

N

G

A

A

A

L

F

L

G

A

G

G

E
|
E

P

M

S

S

G

G

-

H

T

V

W

F

L

F

H

K

P

E

D

R

F

W

C

F

M

G

C

F

P
x
D

D

D

G

G

I

I

L

Y

S

S

A

A

L

A

K

R

V

L

I

L

E

E

I

I

V

L

E

S

K

Q

Y

D

G

Q

P

R

L

D

S

S

K

E

-

H

L

V

L

F

N

S

E

A

I

F

P

P

S

S

-

D

Y

I

P

S

T

T

V

P

R

E

D

I

K

N

I

I

E

T

C

V

E

T

N

E

I

D

Q

S

K

K

T

F

L

A

I

I

M

I

E

E

K

A

V

L

K

Q

E

R

E

-

L

-

P

-

D

D

Y

A

F

Q

E

W

D

G

V

E

N

G

D

N

V

I

N

T

F

T

I

L

D

D

G

G

V

V

R

R

I

V

S

D

M

Y

K

P

D

K

G

G

S

-

W

W

V

G

L

L

I

V

R
|
R

P

A

S
|
S

G
x
N

T
|
T

E

T

S

P

Y

V

I

L

R

V

I

L

T

R

L

F

E

E

G

A

R

D

N

T

V

E

E

E

I

E

A

L

Q

E

S

R

I

I

R

K

T

T

K

V

S

F

R

R

E

N

F

Q

I

L

E

K

G

A

I

V

active site: H101 (= H103), D234 (= D241), D236 (= D243), D238 (= D245), R239 (= R246), D332 (≠ P333)
binding 1-O-phosphono-alpha-D-ribofuranose: Y9 (≠ S12), R12 (= R15), S100 (= S102), T298 (≠ V300), E317 (= E319), R413 (= R416), S415 (= S418), N416 (≠ G419), T417 (= T420)
binding zinc ion: S100 (= S102), D234 (= D241), D236 (= D243), D238 (= D245)

2fkfA Phosphomannomutase/phosphoglucomutase from pseudomonas aeruginosa with alpha-d-glucose 1,6-bisphosphate bound (see paper)
29% identity, 98% coverage: 8:450/451 of query aligns to 5:447/455 of 2fkfA

query
sites
2fkfA

L

I

F

F

G
x
R

T

A

S

Y

G

D

I

I

R
|
R

G

G

K

V

I

V

G

G

S

D

E

T

I

L

T

T

L

A

N

E

L

T

I

A

T

Y

D

W

V

I

G

G

M

R

A

A

A

I

A

G

T

S

Y

E

V

S

G

L

G

A

K

R

G

G

R

E

K

P

A

C

V

V

-

A

I

V

G

G

Y

R

D

D

T

G

R

R

T

L

S

S

N

G

K

P

M

E

V

L

E

V

N

K

A

Q

I

L

I

I

A

Q

G

G

I

L

L

V

Q

D

C

C

G

G

C

C

D

Q

V

V

I

S

R

D

L

V

G

G

M

M

V

V

P

P

T

T

P

P

L

V

V

L

G

Y

Y

Y

A

A

A

A

M

N

K

V

L

L

N

E

A

G

D

K

I

S

G

G

I

V

M

M

I

L

T

T

A

G

S
|
S

H
|
H

N

N

P

P

S

P

Q

D

Y

Y

N

N

G

G

I

F

K
|
K

L

I

W

V

N

V

P

-

K

A

G

G

M

E

A

T

Y

L

T

A

Q

N

D

E

Q

Q

E

I

R

Q

T

A

I

L

E

R

K

E

I

R

V

I

H

E

E

K

K

N

T

D

F

L

S

A

K

S

V

-

S

-

W

-

E

-

H

G

I

V

G

G

D

S

I

V

K

-

D

E

N

Q

S

V

S

D

I

I

I

L

S

P

G

R

Y

Y

M

F

D

K

D

Q

L

I

L

R

N

D

N

D

V

I

D

A

I

M

K

A

G

K

G

P

L

M

K

K

V

V

V

V

V

V

D

D

C

C

A

G

N

N

G

G

A

V

G

A

S

G

F

V

V

I

S

A

P

P

T

Q

V

L

L

I

R

E

K

A

A

L

G

G

C

C

E

S

V

V

L

I

T

P

L

L

N

Y

S

C

Q

E

P

V

D

D

G

G

F

N

F

F

P

P

G

N

R

H

M

H

P

P

E

D

P

P

S

G

E

K

A

P

-

E

N

N

L

L

S

K

E

D

L

L

M

I

K

A

V

K

V

V

K

K

A

A

T

E

G

N

A

A

D

D

I

L

G

G

I

L

A

A

H

F

D
|
D

G

G

D
|
D

A

G

D
|
D

R
|
R

M

V

V

G

A

V

I

V

D

T

D

N

E

T

G

G

K

T

M

I

A

I

D

Y

F

P

D

D

K

R

L

L

L

L

A

M

L

L

-

F

-

A

-

K

-

D

-

V

V

V

S

S

S

R

K

N

I

P

G

G

G

A

K

D

I

I

V

I

T

F

T

D

V

V

D

K

A

C

S

T

F

R

C

R

V

L

D

I

K

A

C

L

M

I

K

S

E

G

S

Y

G

G

G

G

E

R

V

P

V

V

R

M

T

W

K

K

V

T

G

G

D

H

V

S

H

L

V

I

A

K

E

K

A

K

I

M

V

K

E

E

C

T

N

G

A

A

A

L

F

L

G

A

G

G

E

E

P

M

S
|
S

G

G

-
x
H

T

V

W

F

L

F

H

K

P

E

D

R

F

W

C

F

M

G

C

F

P
x
D

D

D

G

G

I

I

L

Y

S

S

A

A

L

A

K

R

V

L

I

L

E

E

I

I

V

L

E

S

K

Q

Y

D

G

Q

P

R

L

D

S

S

K

E

-

H

L

V

L

F

N

S

E

A

I

F

P

P

S

S

-

D

Y

I

P

S

T

T

V

P

R

E

D

I

K

N

I

I

E

T

C

V

E

T

N

E

I

D

Q

S

K

K

T

F

L

A

I

I

M

I

E

E

K

A

V

L

K

Q

E

R

E

-

L

-

P

-

D

D

Y

A

F

Q

E

W

D

G

V

E

N

G

D

N

V

I

N

T

F

T

I

L

D

D

G

G

V

V

R

R

I

V

S

D

M

Y

K

P

D

K

G

G

S

-

W

W

V

G

L

L

I

V

R
|
R

P

A

S
|
S

G
x
N

T
|
T

E

T

S

P

Y

V

I

L

R

V

I

L

T

R

L

F

E

E

G

A

R

D

N

T

V

E

E

E

I

E

A

L

Q

E

S

R

I

I

R

K

T

T

K

V

S

F

R

R

E

N

F

Q

I

L

E

K

G

A

I

V

active site: R12 (= R15), S100 (= S102), H101 (= H103), K110 (= K112), D234 (= D241), D236 (= D243), D238 (= D245), R239 (= R246), H321 (vs. gap), D332 (≠ P333)
binding 1,6-di-O-phosphono-alpha-D-glucopyranose: R7 (≠ G10), H101 (= H103), S319 (= S321), R413 (= R416), S415 (= S418), N416 (≠ G419), T417 (= T420)
binding zinc ion: S100 (= S102), D234 (= D241), D236 (= D243), D238 (= D245)

1pcmX Enzyme-ligand complex of p. Aeruginosa pmm/pgm (see paper)
29% identity, 98% coverage: 8:450/451 of query aligns to 5:447/455 of 1pcmX

query
sites
1pcmX

L

I

F

F

G

R

T

A

S
x
Y

G

D

I

I

R
|
R

G

G

K

V

I

V

G

G

S

D

E

T

I

L

T

T

L

A

N

E

L

T

I

A

T

Y

D

W

V

I

G

G

M

R

A

A

A

I

A

G

T

S

Y

E

V

S

G

L

G

A

K

R

G

G

R

E

K

P

A

C

V

V

-

A

I

V

G

G

Y

R

D

D

T

G

R

R

T

L

S

S

N

G

K

P

M

E

V

L

E

V

N

K

A

Q

I

L

I

I

A

Q

G

G

I

L

L

V

Q

D

C

C

G

G

C

C

D

Q

V

V

I

S

R

D

L

V

G

G

M

M

V

V

P

P

T

T

P

P

L

V

V

L

G

Y

Y

Y

A

A

A

A

M

N

K

V

L

L

N

E

A

G

D

K

I

S

G

G

I

V

M

M

I

L

T

T

A

G

S
|
S

H
|
H

N

N

P

P

S

P

Q

D

Y

Y

N

N

G

G

I

F

K
|
K

L

I

W

V

N

V

P

-

K

A

G

G

M

E

A

T

Y

L

T

A

Q

N

D

E

Q

Q

E

I

R

Q

T

A

I

L

E

R

K

E

I

R

V

I

H

E

E

K

K

N

T

D

F

L

S

A

K

S

V

-

S

-

W

-

E

-

H

G

I

V

G

G

D

S

I

V

K

-

D

E

N

Q

S

V

S

D

I

I

I

L

S

P

G

R

Y

Y

M

F

D

K

D

Q

L

I

L

R

N

D

N

D

V

I

D

A

I

M

K

A

G

K

G

P

L

M

K

K

V

V

V

V

V

V

D

D

C

C

A

G

N

N

G

G

A

V

G

A

S

G

F

V

V

I

S

A

P

P

T

Q

V

L

L

I

R

E

K

A

A

L

G

G

C

C

E

S

V

V

L

I

T

P

L

L

N

Y

S

C

Q

E

P

V

D

D

G

G

F

N

F

F

P

P

G

N

R

H

M

H

P

P

E

D

P

P

S

G

E

K

A

P

-

E

N

N

L

L

S

K

E

D

L

L

M

I

K

A

V

K

V

V

K

K

A

A

T

E

G

N

A

A

D

D

I

L

G

G

I

L

A

A

H

F

D
|
D

G

G

D
|
D

A

G

D
|
D

R
|
R

M

V

V

G

A

V

I

V

D

T

D

N

E

T

G

G

K

T

M

I

A

I

D

Y

F

P

D

D

K

R

L

L

L

L

A

M

L

L

-

F

-

A

-

K

-

D

-

V

V

V

S

S

S

R

K

N

I

P

G

G

G

A

K

D

I

I

V

I

T

F

T

D

V

V

D

K

A

C

S

T

F

R

C

R

V

L

D

I

K

A

C

L

M

I

K

S

E

G

S

Y

G

G

G

G

E

R

V

P

V

V

R

M

T

W

K

K

V
x
T

G
|
G

D
x
H

V

S

H

L

V

I

A

K

E

K

A

K

I

M

V

K

E

E

C

T

N

G

A

A

A

L

F

L

G

A

G

G

E
|
E

P

M

S
|
S

G

G

-
x
H

T

V

W

F

L

F

H

K

P

E

D

R

F

W

C

F

M

G

C

F

P
x
D

D

D

G

G

I

I

L

Y

S

S

A

A

L

A

K

R

V

L

I

L

E

E

I

I

V

L

E

S

K

Q

Y

D

G

Q

P

R

L

D

S

S

K

E

-

H

L

V

L

F

N

S

E

A

I

F

P

P

S

S

-

D

Y

I

P

S

T

T

V

P

R

E

D

I

K

N

I

I

E

T

C

V

E

T

N

E

I

D

Q

S

K

K

T

F

L

A

I

I

M

I

E

E

K

A

V

L

K

Q

E

R

E

-

L

-

P

-

D

D

Y

A

F

Q

E

W

D

G

V

E

N

G

D

N

V

I

N

T

F

T

I

L

D

D

G

G

V

V

R

R

I

V

S

D

M

Y

K

P

D

K

G

G

S

-

W

W

V

G

L

L

I

V

R
|
R

P

A

S
|
S

G

N

T

T

E

T

S

P

Y

V

I

L

R

V

I

L

T

R

L

F

E

E

G

A

R

D

N

T

V

E

E

E

I

E

A

L

Q

E

S

R

I

I

R

K

T

T

K

V

S

F

R

R

E

N

F

Q

I

L

E

K

G

A

I

V

active site: R12 (= R15), S100 (= S102), H101 (= H103), K110 (= K112), D234 (= D241), D236 (= D243), D238 (= D245), R239 (= R246), H321 (vs. gap), D332 (≠ P333)
binding 6-O-phosphono-alpha-D-mannopyranose: Y9 (≠ S12), S100 (= S102), T298 (≠ V300), G299 (= G301), H300 (≠ D302), E317 (= E319), S319 (= S321), H321 (vs. gap), R413 (= R416), S415 (= S418)
binding zinc ion: S100 (= S102), D234 (= D241), D236 (= D243), D238 (= D245)

1p5gX Enzyme-ligand complex of p. Aeruginosa pmm/pgm (see paper)
29% identity, 98% coverage: 8:450/451 of query aligns to 5:447/455 of 1p5gX

query
sites
1p5gX

L

I

F

F

G

R

T

A

S
x
Y

G

D

I

I

R
|
R

G

G

K

V

I

V

G

G

S

D

E

T

I

L

T

T

L

A

N

E

L

T

I

A

T

Y

D

W

V

I

G

G

M

R

A

A

A

I

A

G

T

S

Y

E

V

S

G

L

G

A

K

R

G

G

R

E

K

P

A

C

V

V

-

A

I

V

G

G

Y

R

D

D

T

G

R

R

T

L

S

S

N

G

K

P

M

E

V

L

E

V

N

K

A

Q

I

L

I

I

A

Q

G

G

I

L

L

V

Q

D

C

C

G

G

C

C

D

Q

V

V

I

S

R

D

L

V

G

G

M

M

V

V

P

P

T

T

P

P

L

V

V

L

G

Y

Y

Y

A

A

A

A

M

N

K

V

L

L

N

E

A

G

D

K

I

S

G

G

I

V

M

M

I

L

T

T

A

G

S
|
S

H
|
H

N

N

P

P

S

P

Q

D

Y

Y

N

N

G

G

I

F

K
|
K

L

I

W

V

N

V

P

-

K

A

G

G

M

E

A

T

Y

L

T

A

Q

N

D

E

Q

Q

E

I

R

Q

T

A

I

L

E

R

K

E

I

R

V

I

H

E

E

K

K

N

T

D

F

L

S

A

K

S

V

-

S

-

W

-

E

-

H

G

I

V

G

G

D

S

I

V

K

-

D

E

N

Q

S

V

S

D

I

I

I

L

S

P

G

R

Y

Y

M

F

D

K

D

Q

L

I

L

R

N

D

N

D

V

I

D

A

I

M

K

A

G

K

G

P

L

M

K

K

V

V

V

V

V

V

D

D

C

C

A

G

N

N

G

G

A

V

G

A

S

G

F

V

V

I

S

A

P

P

T

Q

V

L

L

I

R

E

K

A

A

L

G

G

C

C

E

S

V

V

L

I

T

P

L

L

N

Y

S

C

Q

E

P

V

D

D

G

G

F

N

F

F

P

P

G

N

R

H

M

H

P

P

E

D

P

P

S

G

E

K

A

P

-

E

N

N

L

L

S

K

E

D

L

L

M

I

K

A

V

K

V

V

K

K

A

A

T

E

G

N

A

A

D

D

I

L

G

G

I

L

A

A

H

F

D
|
D

G

G

D
|
D

A

G

D
|
D

R
|
R

M

V

V

G

A

V

I

V

D

T

D

N

E

T

G

G

K

T

M

I

A

I

D

Y

F

P

D

D

K

R

L

L

L

L

A

M

L

L

-

F

-

A

-

K

-

D

-

V

V

V

S

S

S

R

K

N

I

P

G

G

G

A

K

D

I

I

V

I

T

F

T

D

V

V

D
x
K

A

C

S

T

F

R

C

R

V

L

D

I

K

A

C

L

M

I

K

S

E

G

S

Y

G

G

G

G

E

R

V

P

V

V

R

M

T

W

K

K

V

T

G
|
G

D
x
H

V

S

H

L

V

I

A

K

E

K

A

K

I

M

V

K

E

E

C

T

N

G

A

A

A

L

F

L

G

A

G

G

E
|
E

P

M

S
|
S

G

G

-
x
H

T

V

W

F

L

F

H

K

P

E

D

R

F

W

C

F

M

G

C

F

P
x
D

D

D

G

G

I

I

L

Y

S

S

A

A

L

A

K

R

V

L

I

L

E

E

I

I

V

L

E

S

K

Q

Y

D

G

Q

P

R

L

D

S

S

K

E

-

H

L

V

L

F

N

S

E

A

I

F

P

P

S

S

-

D

Y

I

P

S

T

T

V

P

R

E

D

I

K

N

I

I

E

T

C

V

E

T

N

E

I

D

Q

S

K

K

T

F

L

A

I

I

M

I

E

E

K

A

V

L

K

Q

E

R

E

-

L

-

P

-

D

D

Y

A

F

Q

E

W

D

G

V

E

N

G

D

N

V

I

N

T

F

T

I

L

D

D

G

G

V

V

R

R

I

V

S

D

M

Y

K

P

D

K

G

G

S

-

W

W

V

G

L

L

I

V

R
|
R

P

A

S
|
S

G
x
N

T
|
T

E

T

S

P

Y

V

I

L

R

V

I

L

T

R

L

F

E

E

G

A

R

D

N

T

V

E

E

E

I

E

A

L

Q

E

S

R

I

I

R

K

T

T

K

V

S

F

R

R

E

N

F

Q

I

L

E

K

G

A

I

V

active site: R12 (= R15), S100 (= S102), H101 (= H103), K110 (= K112), D234 (= D241), D236 (= D243), D238 (= D245), R239 (= R246), H321 (vs. gap), D332 (≠ P333)
binding 6-O-phosphono-alpha-D-glucopyranose: Y9 (≠ S12), S100 (= S102), K277 (≠ D279), G299 (= G301), H300 (≠ D302), E317 (= E319), S319 (= S321), H321 (vs. gap), R413 (= R416), S415 (= S418), N416 (≠ G419), T417 (= T420)
binding zinc ion: S100 (= S102), D234 (= D241), D236 (= D243), D238 (= D245)

1p5dX Enzyme-ligand complex of p. Aeruginosa pmm/pgm (see paper)
29% identity, 98% coverage: 8:450/451 of query aligns to 5:447/455 of 1p5dX

query
sites
1p5dX

L

I

F

F

G

R

T

A

S
x
Y

G

D

I

I

R
|
R

G

G

K

V

I

V

G

G

S

D

E

T

I

L

T

T

L

A

N

E

L

T

I

A

T

Y

D

W

V

I

G

G

M

R

A

A

A

I

A

G

T

S

Y

E

V

S

G

L

G

A

K

R

G

G

R

E

K

P

A

C

V

V

-

A

I

V

G

G

Y

R

D

D

T

G

R

R

T

L

S

S

N

G

K

P

M

E

V

L

E

V

N

K

A

Q

I

L

I

I

A

Q

G

G

I

L

L

V

Q

D

C

C

G

G

C

C

D

Q

V

V

I

S

R

D

L

V

G

G

M

M

V

V

P

P

T

T

P

P

L

V

V

L

G

Y

Y

Y

A

A

A

A

M

N

K

V

L

L

N

E

A

G

D

K

I

S

G

G

I

V

M

M

I

L

T

T

A

G

S
|
S

H
|
H

N

N

P

P

S

P

Q

D

Y

Y

N

N

G

G

I

F

K
|
K

L

I

W

V

N

V

P

-

K

A

G

G

M

E

A

T

Y

L

T

A

Q

N

D

E

Q

Q

E

I

R

Q

T

A

I

L

E

R

K

E

I

R

V

I

H

E

E

K

K

N

T

D

F

L

S

A

K

S

V

-

S

-

W

-

E

-

H

G

I

V

G

G

D

S

I

V

K

-

D

E

N

Q

S

V

S

D

I

I

I

L

S

P

G

R

Y

Y

M

F

D

K

D

Q

L

I

L

R

N

D

N

D

V

I

D

A

I

M

K

A

G

K

G

P

L

M

K

K

V

V

V

V

V

V

D

D

C

C

A

G

N

N

G

G

A

V

G

A

S

G

F

V

V

I

S

A

P

P

T

Q

V

L

L

I

R

E

K

A

A

L

G

G

C

C

E

S

V

V

L

I

T

P

L

L

N

Y

S

C

Q

E

P

V

D

D

G

G

F

N

F

F

P

P

G

N

R

H

M

H

P

P

E

D

P

P

S

G

E

K

A

P

-

E

N

N

L

L

S

K

E

D

L

L

M

I

K

A

V

K

V

V

K

K

A

A

T

E

G

N

A

A

D

D

I

L

G

G

I

L

A

A

H

F

D
|
D

G

G

D
|
D

A

G

D
|
D

R
|
R

M

V

V

G

A

V

I

V

D

T

D

N

E

T

G

G

K

T

M

I

A

I

D

Y

F

P

D

D

K

R

L

L

L

L

A

M

L

L

-

F

-

A

-

K

-

D

-

V

V

V

S

S

S

R

K

N

I

P

G

G

G

A

K

D

I

I

V

I

T

F

T

D

V

V

D

K

A

C

S

T

F

R

C

R

V

L

D

I

K

A

C

L

M

I

K

S

E

G

S

Y

G

G

G

G

E

R

V

P

V

V

R

M

T

W

K

K

V
x
T

G
|
G

D
x
H

V

S

H

L

V

I

A

K

E

K

A

K

I

M

V

K

E

E

C

T

N

G

A

A

A

L

F

L

G

A

G

G

E
|
E

P

M

S
|
S

G

G

-
x
H

T

V

W

F

L

F

H

K

P

E

D

R

F

W

C

F

M

G

C

F

P
x
D

D

D

G

G

I

I

L

Y

S

S

A

A

L

A

K

R

V

L

I

L

E

E

I

I

V

L

E

S

K

Q

Y

D

G

Q

P

R

L

D

S

S

K

E

-

H

L

V

L

F

N

S

E

A

I

F

P

P

S

S

-

D

Y

I

P

S

T

T

V

P

R

E

D

I

K

N

I

I

E

T

C

V

E

T

N

E

I

D

Q

S

K

K

T

F

L

A

I

I

M

I

E

E

K

A

V

L

K

Q

E

R

E

-

L

-

P

-

D

D

Y

A

F

Q

E

W

D

G

V

E

N

G

D

N

V

I

N

T

F

T

I

L

D

D

G

G

V

V

R

R

I

V

S

D

M

Y

K

P

D

K

G

G

S

-

W

W

V

G

L

L

I

V

R
|
R

P

A

S
|
S

G

N

T
|
T

E

T

S

P

Y

V

I

L

R

V

I

L

T

R

L

F

E

E

G

A

R

D

N

T

V

E

E

E

I

E

A

L

Q

E

S

R

I

I

R

K

T

T

K

V

S

F

R

R

E

N

F

Q

I

L

E

K

G

A

I

V

active site: R12 (= R15), S100 (= S102), H101 (= H103), K110 (= K112), D234 (= D241), D236 (= D243), D238 (= D245), R239 (= R246), H321 (vs. gap), D332 (≠ P333)
binding 1-O-phosphono-alpha-D-glucopyranose: Y9 (≠ S12), S100 (= S102), R239 (= R246),