SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_048081170.1 NCBI__GCF_000745485.1:WP_048081170.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 13 hits to proteins with known functional sites (download)

2y27B Crystal structure of paak1 in complex with atp from burkholderia cenocepacia (see paper)
50% identity, 98% coverage: 8:430/432 of query aligns to 8:427/427 of 2y27B

query
sites
2y27B

E

E

C

T

M

A

S

S

E

R

N

D

D

E

K

L

E

T

A

A

L

L

Q

Q

L

L

K

E

R

R

L

L

Q

K

A

W

V

S

V

L

K

R

R

H

A

A

Y

Y

E

D

N

H

V

S

P

P

Y

V

Y

Y

R

R

K

R

R

K

F

F

D

D

E

E

A

A

G

G

V

V

K

H

P

P

E

D

D

D

I

L

K

K

T

T

L

L

K

A

D

D

I

L

E

S

K

R

L

F

P

P

F

F

T

T

T

T

K
|
K

T

G

D

D

L

L

R

R

D

D

A

S

Y

Y

P

P

F

F

G

G

M

M

F

F

A

A

V

V

P

P

E

Q

D

D

D

R

I

I

V

S

E

R

V

I

H

H

T

A

T
x
S

S
|
S

G
|
G

T
|
T

T
|
T

G

G

K

K

P

P

T

T

V

V

S

V

G

G

Y

Y

T

T

V

A

N

A

D

D

L

I

D

D

L

T

W

W

G

A

E

N

V

L

I

V

A

A

R

R

A

S

L

I

G

R

M

A

A

A

G

G

A

A

G

R

K

R

K

G

D

D

I

K

I

V

Q

H

N

V

A

S

Y

Y

G

G

Y

Y

G

G

L

L

F
|
F

T

T

G

G

G

G

M

L

G

G

V

A

H

H

Y

Y

G

G

A

A

Q

E

K

R

I

A

G

G

A

L

T

T

V

V

V

I

P

P

I

F

S

G

A

G

G

G

N

Q

T

T

M

E

R

K

Q

Q

L

V

E

Q

I

L

M

I

E

Q

D

D

F

F

G

R

S

P

T

D

V

I

L

I

T

M

C

V

T

T

P

P

S

S

Y

Y

A

M

L

L

Y

S

L

I

G

A

E

D

M

E

A

I

E

E

R

R

E

Q

G

G

I

L

S

D

R

P

E
x
V

S

Q

I
x
S

K

S

L
|
L

K

R

A

I

G

G

V

I

F

F

G

G

A
|
A

E
|
E

M
x
P

W

W

T

T

E

N

E

D

M

M

R

R

N

V

E

A

I

I

E

E

K

Q

R

R

L
x
M

N
x
G

L

I

T

D

A

A

L

V

N
x
D

I
|
I

Y
|
Y

G
|
G

L
|
L

T
x
S

E

E

V

V

I

M

G

G

P

P

G

G

V

V

A

A

M

S

E

E

C

C

-

V

E

E

D

T

K

K

N

D

G

G

L

P

H

T

I

I

S

W

D

E

D

D

H

H

F

F

Y

Y

P

P

E

E

I

I

V

I

D

D

S

P

K

E

T

T

L

G

E

E

T

V

L

L

P

P

E

D

G

G

E

E

K

L

G

G

E

E

L

L

V

V

L

F

T

T

T

S

L

L

T

T

R

K

E

E

G

A

M

L

P

P

V

I

I

I

R

R

F

Y

R

R

T

T

K

R

D
|
D

V

L

T

T

A

R

L

L

R

L

K

P

G

G

K

-

C

-

S

-

C

T

G

A

R

R

T

T

L

M

I

R

K

R

M

M

D

E

R

K

I
|
I

T

T

G

G

R
|
R

T

S

D

D

D

D

M

M

L

M

K

I

I

V

R

R

G

G

V

V

I

N

V

V

F

F

P

P

S

T

Q

Q

I

I

E

E

K

E

A

Q

L

L

L

L

K

K

I
x
Q

E

R

D

A

L
|
L

E

A

P

P

H

H

Y

Y

Q

Q

I

I

V

V

V

L

T

T

R

K

P

E

Q

G

H

P

L

L

D

D

E

V

L

L

E

T

V

L

Q

N

V

V

E

E

T

P

S

C

E

P

K

E

F

T

F

A

S

P

D

D

E

T

V

A

K

A

H

-

V

I

E

Q

E

V

V

A

K

K

K

Q

M

A

I

L

E

A

D

Y

R

D

I

I

H

K

S

S

E

L

I

I

G

G

L

V

R

T

V

A

N

V

V

I

T

N

L

V

V

L

E

P

P

V

Q

N

S

G

L

I

P

E

R

R

S

S

E

V

G

G

K
|
K

A

A

V

R

R

R

V

V

I

V

D

D

K

K

R

R

binding adenosine-5'-triphosphate: K65 (= K65), S90 (≠ T90), S91 (= S91), G92 (= G92), T93 (= T93), T94 (= T94), F138 (= F138), A211 (= A211), E212 (= E212), P213 (≠ M213), D232 (≠ N232), I233 (= I233), Y234 (= Y234), G235 (= G235), L236 (= L236), S237 (≠ T237), D302 (= D301), I320 (= I322), R323 (= R325), K419 (= K422)
binding magnesium ion: V200 (≠ E200), S202 (≠ I202), L204 (= L204), M226 (≠ L226), G227 (≠ N227), Q347 (≠ I349), L350 (= L352)

2y4oA Crystal structure of paak2 in complex with phenylacetyl adenylate (see paper)
49% identity, 98% coverage: 8:431/432 of query aligns to 10:433/433 of 2y4oA

query
sites
2y4oA

E

E

C

T

M

A

S

S

E

R

N

D

D

E

K

L

E

Q

A

A

L

L

Q

Q

L

L

K

E

R

R

L

L

Q

K

A

W

V

S

V

L

K

R

R

H

A

A

Y

Y

E

D

N

N

V

V

P

P

Y

H

Y

Y

R

R

K

R

R

T

F

F

D

D

E

A

A

A

G

G

V

V

K

H

P

P

E

D

D

D

I

L

K

K

T

S

L

L

K

A

D

D

I

L

E

A

K

K

L

F

P

P

F

F

T

S

T

T

K

K

T

N

D

D

L

L

R

R

D

D

A

N

Y

Y

P

P

F

F

G

G

M

L

F

F

A

A

V

V

P

P

E

R

D

E

D

Q

I

V

V

V

E

R

V

V

H

H

T

A

T

S

S

S

G

G

T

T

T

T

G

G

K

K

P

P

T

T

V

V

S

V

G

G

Y

Y

T

T

V

A

N

R

D

D

L

I

D

D

L

T

W

W

G

A

E

N

V

V

I

T

A

A

R

R

A

S

L

I

G

R

M

A

A

A

G

G

A

G

G

R

K

P

K

G

D

D

I

T

I

L

Q

H

N

N

A

A

Y
x
F

G

G

Y

Y

G

G

L

L

F
|
F

T

T

G

G

G

G

M

L

G

G

V

I

H

H

Y

Y

G

G

A

A

Q

E

K

R

I

L

G

G

A

C

T

M

V

V

V

V

P

P

I

M

S

S

A

G

G

G

N

Q

T

T

M

E

R

K

Q

Q

L

V

E

Q

I

L

M

I

E

R

D

D

F

F

G

E

S

P

T

K

V

I

L

I

T

L

C

V

T

T

P

P

S

S

Y

Y

A

M

L

L

Y

N

L

L

G

I

E

D

M

E

A

M

E

V

R

R

E

Q

G

G

I

M

S

D

R

P

E

A

S

E

I

S

K

S

L

L

K

K

A

I

G

G

V

I

F

F

G

G

A
|
A

E
|
E

M
x
P

W

W

T

T

E

Q

E

A

M

L

R

R

N

N

E

E

I

V

E

E

K

T

R

R

L

V

N

G

L

I

T

D

A

A

L

L

N

D

I
|
I

Y

Y

G
|
G

L
|
L

T
x
S

E

E

V

V

I

M

G

G

P
|
P

G

G

V

V

A

A

M

C

E

E

C

C

-

V

E

E

D

T

K

K

N

D

G

G

L

P

H

V

I

I

S

W

D

E

D

D

H

H

F

F

Y

Y

P

P

E

E

I

I

V

I

D

D

S

P

K

V

T

T

L

G

E

E

T

V

L

L

P

P

E

D

G

G

E

S

K

Q

G

G

E

E

L

L

V

V

L

F

T

T

T

S

L

L

T

T

R

K

E

E

G

A

M

M

P

P

V

V

I

I

R

R

F

Y

R

R

T

T

K

R

D
|
D

V

L

T

T

A

A

L

L

R

L

K

P

G

-

K

-

C

-

S

P

C

T

G

A

R

R

T

A

L

M

I

R

K

R

M

L

D

A

R

K

I

I

T

T

G

G

R
|
R

T

S

D

D

D

D

M

M

L

L

K
x
I

I

V

R

R

G

G

V

V

I
x
N

V

V

F

F

P

P

S

S

Q

Q

I

I

E

E

K

E

A

I

L

V

L

V

K

A

I

L

E

P

D

L

L

L

E

S

P

G

H

Q

Y

F

Q

Q

I

I

V

T

V

L

T

S

R

R

P

D

Q

G

H

H

L

M

D

D

E

R

L

L

E

D

V

L

Q

A

V

V

E

E

T

L

-

R

S

S

E

E

K

A

F

A

F

A

S

S

D

V

E

T

V

D

K

G

H

E

V

R

E

A

E

A

V

L

K

A

K

R

M

E

I

L

E

Q

D

H

R

R

I

I

H

K

S

T

E

M

I

V

G

G

L

V

R

S

V

S

N

G

V

V

T

T

L

V

V

L

E

A

P

A

Q

G

S

G

L

I

P

P

R

A

S

T

E

A

-

T

G

G

K

K

A

A

V

R

R

R

V

V

I

I

D

D

K

R

R

R

E

Q

binding 5'-o-[hydroxy(phenylacetyl)phosphoryl]adenosine: F135 (≠ Y133), F140 (= F138), A213 (= A211), E214 (= E212), P215 (≠ M213), I235 (= I233), G237 (= G235), L238 (= L236), S239 (≠ T237), P244 (= P242), D304 (= D301), R325 (= R325), I331 (≠ K331), N336 (≠ I336)

2y4oB Crystal structure of paak2 in complex with phenylacetyl adenylate (see paper)
49% identity, 98% coverage: 8:430/432 of query aligns to 10:432/432 of 2y4oB

query
sites
2y4oB

E

E

C

T

M

A

S

S

E

R

N

D

D

E

K

L

E

Q

A

A

L

L

Q

Q

L

L

K

E

R

R

L

L

Q

K

A

W

V

S

V

L

K

R

R

H

A

A

Y

Y

E

D

N

N

V

V

P

P

Y

H

Y

Y

R

R

K

R

R

T

F

F

D

D

E

A

A

A

G

G

V

V

K

H

P

P

E

D

D

D

I

L

K

K

T

S

L

L

K

A

D

D

I

L

E

A

K

K

L

F

P

P

F

F

T

S

T

T

K

K

T

N

D

D

L

L

R

R

D

D

A

N

Y

Y

P

P

F

F

G

G

M

L

F

F

A

A

V

V

P

P

E

R

D

E

D

Q

I

V

V

V

E

R

V

V

H

H

T

A

T

S

S

S

G

G

T

T

T

T

G

G

K

K

P

P

T

T

V

V

S

V

G

G

Y

Y

T

T

V

A

N

R

D

D

L

I

D

D

L

T

W

W

G

A

E

N

V

V

I

T

A

A

R

R

A

S

L

I

G

R

M

A

A

A

G

G

A

G

G

R

K

P

K

G

D

D

I

T

I

L

Q

H

N

N

A

A

Y
x
F

G

G

Y

Y

G

G

L

L

F
|
F

T

T

G

G

G

G

M

L

G

G

V

I

H

H

Y

Y

G

G

A

A

Q

E

K

R

I

L

G

G

A

C

T

M

V

V

V

V

P

P

I

M

S

S

A

G

G

G

N

Q

T

T

M

E

R

K

Q

Q

L

V

E

Q

I

L

M

I

E

R

D

D

F

F

G

E

S

P

T

K

V

I

L

I

T

L

C

V

T

T

P

P

S

S

Y

Y

A

M

L

L

Y

N

L

L

G

I

E

D

M

E

A

M

E

V

R

R

E

Q

G

G

I

M

S

D

R

P

E

A

S

E

I
x
S

K

S

L

L

K

K

A

I

G

G

V

I

F

F

G
|
G

A
|
A

E
|
E

M
x
P

W

W

T

T

E

Q

E

A

M

L

R

R

N

N

E

E

I

V

E

E

K

T

R

R

L
x
V

N

G

L

I

T

D

A

A

L

L

N

D

I
|
I

Y

Y

G
|
G

L
|
L

T
x
S

E

E

V

V

I

M

G

G

P
|
P

G

G

V

V

A

A

M

C

E

E

C

C

-

V

E

E

D

T

K

K

N

D

G

G

L

P

H

V

I

I

S

W

D

E

D

D

H

H

F

F

Y

Y

P

P

E

E

I

I

V

I

D

D

S

P

K

V

T

T

L

G

E

E

T

V

L

L

P

P

E

D

G

G

E

S

K

Q

G

G

E

E

L

L

V

V

L

F

T

T

T

S

L

L

T

T

R

K

E

E

G

A

M

M

P

P

V

V

I

I

R

R

F

Y

R

R

T

T

K

R

D
|
D

V

L

T

T

A

A

L

L

R

L

K

P

G

-

K

-

C

-

S

P

C

T

G

A

R

R

T

A

L

M

I

R

K

R

M

L

D

A

R

K

I

I

T

T

G

G

R
|
R

T

S

D

D

D

D

M

M

L

L

K
x
I

I

V

R

R

G

G

V

V

I
x
N

V

V

F

F

P

P

S

S

Q

Q

I

I

E

E

K

E

A

I

L

V

L

V

K

A

I

L

E

P

D

L

L

L

E

S

P

G

H

Q

Y

F

Q

Q

I

I

V

T

V

L

T

S

R

R

P

D

Q

G

H

H

L

M

D

D

E

R

L

L

E

D

V

L

Q

A

V

V

E

E

T

L

-

R

S

S

E

E

K

A

F

A

F

A

S

S

D

V

E

T

V

D

K

G

H

E

V

R

E

A

E

A

V

L

K

A

K

R

M

E

I

L

E

Q

D

H

R

R

I

I

H

K

S

T

E

M

I

V

G

G

L

V

R

S

V

S

N

G

V

V

T

T

L

V

V

L

E

A

P

A

Q

G

S

G

L

I

P

P

R

A

S

T

E

A

-

T

G

G

K

K

A

A

V

R

R

R

V

V

I

I

D

D

K

R

R

R

binding 5'-o-[hydroxy(phenylacetyl)phosphoryl]adenosine: F135 (≠ Y133), F140 (= F138), G212 (= G210), A213 (= A211), E214 (= E212), P215 (≠ M213), I235 (= I233), G237 (= G235), L238 (= L236), S239 (≠ T237), P244 (= P242), D304 (= D301), R325 (= R325), I331 (≠ K331), N336 (≠ I336)
binding magnesium ion: S204 (≠ I202), V228 (≠ L226)

2y4nA Paak1 in complex with phenylacetyl adenylate (see paper)
49% identity, 98% coverage: 8:431/432 of query aligns to 8:426/426 of 2y4nA

query
sites
2y4nA

E

E

C

T

M

A

S

S

E

R

N

D

D

E

K

L

E

T

A

A

L

L

Q

Q

L

L

K

E

R

R

L

L

Q

K

A

W

V

S

V

L

K

R

R

H

A

A

Y

Y

E

D

N

H

V

S

P

P

Y

V

Y

Y

R

R

K

R

R

K

F

F

D

D

E

E

A

A

G

G

V

V

K

H

P

P

E

D

D

D

I

L

K

K

T

T

L

L

K

A

D

D

I

L

E

S

K

R

L

F

P

P

F

F

T

T

T

T

K

K

T

G

D

D

L

L

R

R

D

D

A

S

Y

Y

P

P

F

F

G

G

M

M

F

F

A

A

V

V

P

P

E

Q

D

D

D

R

I

I

V

S

E

R

V

I

H

H

T

A

T

S

S

S

G

G

T

-

T

-

G

G

K

K

P

P

T

T

V

V

S

V

G

G

Y

Y

T

T

V

A

N

A

D

D

L

I

D

D

L

T

W

W

G

A

E

N

V

L

I

V

A

A

R

R

A

S

L

I

G

R

M

A

A

A

G

G

A

A

G

R

K

R

K

G

D

D

I

K

I

V

Q

H

N

V

A

S

Y
|
Y

G

G

Y

Y

G

G

L

L

F
|
F

T

T

G
|
G

G

G

M

L

G

G

V

A

H

H

Y

Y

G

G

A

A

Q

E

K

R

I

A

G

G

A

L

T

T

V

V

V

I

P

P

I

F

S

G

A

G

G

G

N

Q

T

T

M

E

R

K

Q

Q

L

V

E

Q

I

L

M

I

E

Q

D

D

F

F

G

R

S

P

T

D

V

I

L

I

T

M

C

V

T

T

P

P

S

S

Y

Y

A

M

L

L

Y

S

L

I

G

A

E

D

M

E

A

I

E

E

R

R

E

Q

G

G

I

L

S

D

R

P

E
x
V

S

Q

I
x
S

K

S

L

L

K

R

A

I

G

G

V

I

F

F

G
|
G

A
|
A

E
|
E

M
x
P

W

W

T

T

E

N

E

D

M

M

R

R

N

V

E

A

I

I

E

E

K

Q

R

R

L

M

N

G

L

I

T

D

A

A

L

V

N

D

I
|
I

Y
|
Y

G
|
G

L
|
L

T
x
S

E

E

V

V

I

M

G

G

P
|
P

G

G

V

V

A

A

M

S

E

E

C

C

-

V

E

E

D

T

K

K

N

D

G

G

L

P

H

T

I

I

S

W

D

E

D

D

H

H

F

F

Y

Y

P

P

E

E

I

I

V

I

D

D

S

P

K

E

T

T

L

G

E

E

T

V

L

L

P

P

E

D

G

G

E

E

K

L

G

G

E

E

L

L

V

V

L

F

T

T

T

S

L

L

T

T

R

K

E

E

G

A

M

L

P

P

V

I

I

I

R

R

F

Y

R

R

T

T

K

R

D
|
D

V

L

T

T

A

R

L

L

R

L

K

P

G

G

K

-

C

-

S

-

C

T

G

A

R

R

T

T

L

M

I

R

K

R

M

M

D

E

R

K

I

I

T

T

G

G

R
|
R

T

S

D

D

D

D

M

M

L

M

K

I

I

V

R

R

G

G

V

V

I

N

V

V

F

F

P

P

S

T

Q

Q

I

I

E

E

K

E

A

Q

L

L

L

L

K

K

I
x
Q

E

R

D

A

L
|
L

E

A

P

P

H

H

Y

Y

Q

Q

I

I

V

V

V

L

T

T

R

K

P

E

Q

G

H

P

L

L

D

D

E

V

L

L

E

T

V

L

Q

N

V

V

E

E

T

P

S

C

E

P

K

E

F

T

F

A

S

P

D

D

E

T

V

A

K

A

H

-

V

I

E

Q

E

V

V

A

K

K

K

Q

M

A

I

L

E

A

D

Y

R

D

I

I

H

K

S

S

E

L

I

I

G

G

L

V

R

T

V

A

N

V

V

I

T

N

L

V

V

L

E

P

P

V

Q

N

S

G

L

I

P

E

R

R

S

S

E

V

G

G

K
|
K

A

A

V

R

R

R

V

V

I

V

D

D

K

K

R

R

E

K

binding 5'-o-[hydroxy(phenylacetyl)phosphoryl]adenosine: Y131 (= Y133), F136 (= F138), G138 (= G140), G208 (= G210), A209 (= A211), E210 (= E212), P211 (≠ M213), I231 (= I233), Y232 (= Y234), G233 (= G235), L234 (= L236), S235 (≠ T237), P240 (= P242), D300 (= D301), R321 (= R325), K417 (= K422)
binding magnesium ion: V198 (≠ E200), S200 (≠ I202), Q345 (≠ I349), L348 (= L352)

4r1mA Crystal structure of a putative acyl-coa ligase (bt_0428) from bacteroides thetaiotaomicron vpi-5482 at 2.48 a resolution
47% identity, 99% coverage: 3:431/432 of query aligns to 7:434/435 of 4r1mA

query
sites
4r1mA

W

W

N

E

K

E

E

E

A

I

E

E

C

I

M

M

S

S

E

R

N

E

D

K

K

L

E

Q

A

E

L

L

Q

Q

L

L

K

Q

R

R

L

L

Q

K

A

K

V

T

V

I

K

N

R

I

A

A

Y

-

E

A

N

N

V

S

P

P

Y

Y

Y

Y

R

K

K

E

R

V

F

F

D

S

E

K

A

N

G

G

V

I

K

T

P

G

E

D

D

S

I

I

K

Q

T

S

L

L

K

D

D

D

I

I

E

R

K

K

L

I

P

P

F

F

T

T

T

T

K

K

T

S

D

D

L

M

R

R

D

A

A

N

Y

Y

P

P

F

F

G

G

M

L

F

V

A

A

V

G

P

D

-

M

E

K

D

R

D

D

I

G

V

V

E

R

V

I

H

H

T

S

T

S

S

S

G

G

T

T

T

T

G

G

K

N

P

P

T

T

V

V

S

I

G

V

Y

H

T

S

V

Q

N

H

D

D

L

L

D

D

L

S

W

W

G

A

E

N

V

L

I

V

A

A

R

R

A

C

L

L

G

Y

M

M

A

V

G

G

A

I

G

R

K

K

K

T

D

D

I

V

I

F

Q

Q

N

N

A

S

Y

S

G

G

Y

Y

G

G

L

M

F

F

T

T

G

G

G

G

M

L

G

G

V

F

H

Q

Y

Y

G

G

A

A

Q

E

K

R

I

L

G

G

A

C

T

L

V

T

V

V

P

P

I

A

S

A

A

A

G

G

N

N

T

S

M

K

R

R

Q

Q

L

I

E

K

I

F

M

I

E

S

D

D

F

F

G

K

S

T

T

T

V

A

L

L

T

H

C

A

T

I

P

P

S

S

Y

Y

A

A

L

I

Y

R

L

L

G

A

E

E

M

V

A

F

E

Q

R

E

E

E

G

G

I

I

S

D

R

P

E

R

S

E

I

T

K

T

L

L

K

K

A

T

G

L

V

V

F

I

G

G

A
|
A

E
|
E

M
x
P

W

H

T

T

E

D

E

E

M

Q

R

R

N

R

E

K

I

I

E

E

K

R

R

M

L

L

N

N

L

V

T

K

A

A

L

Y

N
|
N

I
x
S

Y
x
F

G
|
G

L
x
M

T
|
T

E

E

V

M

I

N

G

G

P

P

G

G

V

V

A

A

M

F

E

E

C
|
C

E

Q

D

E

K

Q

N

N

G

G

L

M

H
|
H

I

F

S

W

D

E

D

D

H

C

F

Y

Y

L

P

V

E

E

I

I

V

I

D

D

S

P

K

E

T

T

L

G

E

E

T

P

L

V

P

P

E

E

G

G

E

E

K

I

G

G

E

E

L

L

V

V

L

L

T

T

T

T

L

L

T

D

R

R

E

E

G

M

M

M

P

P

V

L

I

I

R

R

F

Y

R

R

T

T

K

R

D
|
D

V

L

T

T

A

R

L

I

R

L

K

P

G

G

K

K

C
|
C

S

P

C
|
C

G

G

R

R

T

T

L

H

I

L

K

R

M

I

D

D

R

R

I

I

T

K

G

G

R
|
R

T

S

D

D

D

D

M

M

L

F

K
x
I

I

I

R

K

G

G

V

V

I
x
N

V

I

F

F

P

P

S

M

Q

Q

I

V

E

E

K

K

A

I

L

L

L

V

K

Q

I

F

E

P

D

E

L

L

E

G

P

S

H

N

Y

Y

Q

L

I

I

V

T

V

L

T

E

R

T

P

V

Q

N

H

N

L

Q

D

D

E

E

L

M

E

I

V

V

Q

E

V

V

E

E

T

L

S

S

E

D

K

-

F

L

F

S

S

T

D

D

E

N

V

Y

K

I

H

E

V

L

E

E

E

K

V

I

K

R

K

R

M

D

I

I

E

I

D

R

R

Q

I

L

H

K

S

D

E

E

I

I

G

L

L

V

R

T

V

P

N

K

V

V

T

K

L

L

V

V

E

K

P

K

Q

G

S

S

L

L

P

P

R

Q

S

S

E

E

G

G

K

K

A

A

V

V

R

R

V

V

I

K

D

D

K

L

R

R

E

D

binding adenosine monophosphate: A215 (= A211), E216 (= E212), P217 (≠ M213), N236 (= N232), S237 (≠ I233), F238 (≠ Y234), G239 (= G235), M240 (≠ L236), T241 (= T237), D305 (= D301), R329 (= R325), I335 (≠ K331), N340 (≠ I336)
binding zinc ion: C252 (= C248), H259 (= H255), C314 (= C310), C316 (= C312)

4r1lA Crystal structure of a putative acyl-coa ligase (bt_0428) from bacteroides thetaiotaomicron vpi-5482 at 2.42 a resolution
46% identity, 99% coverage: 3:431/432 of query aligns to 7:432/433 of 4r1lA

query
sites
4r1lA

W

W

N

E

K

E

E

E

A

I

E

E

C

I

M

M

S

S

E

R

N

E

D

K

K

L

E

Q

A

E

L

L

Q

Q

L

L

K

Q

R

R

L

L

Q

K

A

K

V

T

V

I

K

N

R

I

A

A

Y

-

E

A

N

N

V

S

P

P

Y

Y

Y

Y

R

K

K

E

R

V

F

F

D

S

E

K

A

N

G

G

V

I

K

T

P

G

E

D

D

S

I

I

K

Q

T

S

L

L

K

D

D

D

I

I

E

R

K

K

L

I

P

P

F

F

T

T

T

T

K

K

T

S

D

D

L

M

R

R

D

A

A

N

Y

Y

P

P

F

F

G

G

M

L

F

V

A

A

V

G

P

D

-

M

E

K

D

R

D

D

I

G

V

V

E

R

V

I

H

H

T

S

T

S

S

S

G

G

T

T

T

T

G

G

K

N

P

P

T

T

V

V

S

I

G

V

Y

H

T

S

V

Q

N

H

D

D

L

L

D

D

L

S

W

W

G

A

E

N

V

L

I

V

A

A

R

R

A

C

L

L

G

Y

M

M

A

V

G

G

A

I

G

R

K

K

K

T

D

D

I

V

I

F

Q

Q

N

N

A
x
S

Y

S

G

G

Y

Y

G

G

L

M

F

F

T

T

G

G

G

G

M

L

G

G

V

F

H

Q

Y

Y

G

G

A

A

Q

E

K

R

I

L

G

G

A

C

T

L

V

T

V

V

P

P

I

A

S

A

A
|
A

G
|
G

N
|
N

T
x
S

M

K

R

R

Q

Q

L

I

E

K

I

F

M

I

E

S

D

D

F

F

G

K

S

T

T

T

V

A

L

L

T

H

C

A

T
x
I

P

P

S

S

Y
|
Y

A

A

L

I

Y

R

L

L

G

A

E

E

M

V

A

F

E

Q

R

E

E

E

G

G

I

I

S

D

R

P

E

R

S

E

I

T

K

T

L

L

K

K

A

T

G

L

V

V

F

I

G

G

A
|
A

E
|
E

M
x
P

W

H

T

T

E

D

E

E

M

Q

R

R

N

R

E

K

I

I

E

E

K

R

R

M

L

L

N

N

L

V

T

K

A

A

L

Y

N

N

I
x
S

Y
x
F

G
|
G

L
x
M

T
|
T

E

E

V

M

I

N

G

G

P

P

G

G

V

V

A

A

M

F

E

E

C
|
C

E

Q

D

E

K

Q

N

N

G

G

L

M

H
|
H

I

F

S

W

D

E

D

D

H

C

F

Y

Y

L

P

V

E

E

I

I

V

I

D

D

S

P

K

E

T

T

L

G

E

E

T

P

L

V

P

P

E

E

G

G

E

E

K

I

G

G

E

E

L

L

V

V

L

L

T

T

T

T

L

L

T

D

R

R

E

E

G

M

M

M

P

P

V

L

I

I

R

R

F

Y

R

R

T

T

K

R

D
|
D

V

L

T

T

A

R

L

I

R

L

K

P

G

G

K

K

C
|
C

S

P

C
|
C

G

G

R

R

T

T

L

H

I

L

K

R

M

I

D

D

R

R

I

I

T

K

G

G

R
|
R

T

S

D

D

D

D

M

M

L

F

K

I

I

I

R
x
K

G

G

V

V

I
x
N

V

I

F

F

P

P

S

M

Q

Q

I

V

E

E

K

K

A

I

L

L

L

V

K

Q

I

F

E

P

D

E

L

L

E

G

P

S

H

N

Y

Y

Q

L

I

I

V

T

V

L

T

E

R

T

P

V

Q

N

H

N

L

Q

D

D

E

E

L

M

E

I

V

V

Q

E

V

V

E

E

T

L

S

S

E

D

K

-

F

L

F

S

S

T

D

D

E

N

V

Y

K

I

H

E

V

L

E

E

E

K

V

I

K

R

K

R

M

D

I

I

E

I

D

R

R

Q

I

L

H

K

S

D

E

E

I

I

G

L

L

V

R
x
T

V

P

N

K

V

V

T

K

L

L

V

V

E

K

P

K

Q

G

S

S

L

L

P

P

R

Q

S

S

E

E

G

-

K

-

A

A

V

V

R

R

V

V

I

K

D

D

K

L

R

R

E

D

binding adenosine-5'-diphosphate: A215 (= A211), E216 (= E212), P217 (≠ M213), S237 (≠ I233), F238 (≠ Y234), G239 (= G235), M240 (≠ L236), T241 (= T237), D305 (= D301), R329 (= R325), N340 (≠ I336)
binding adenosine monophosphate: A215 (= A211), E216 (= E212), P217 (≠ M213), S237 (≠ I233), F238 (≠ Y234), G239 (= G235), M240 (≠ L236), T241 (= T237), D305 (= D301), R329 (= R325), N340 (≠ I336)
binding coenzyme a: S136 (≠ A132), A164 (= A160), G165 (= G161), N166 (= N162), S167 (≠ T163), I185 (≠ T181), Y188 (= Y184), K337 (≠ R333), T408 (≠ R405)
binding zinc ion: C252 (= C248), H259 (= H255), C314 (= C310), C316 (= C312)

6he0A Crystal structure of 2-hydroxyisobutyryl-coa ligase (hcl) in complex with 2-hib-amp and coa in the thioesterfication state (see paper)
32% identity, 100% coverage: 3:432/432 of query aligns to 25:466/477 of 6he0A

query
sites
6he0A

W

W

N

F

K

P

E

R

A

R

E

E

C

T

M

M

S

P

E

A

N

A

D

E

K

R

E

D

A

K

L

A

Q

I

L

L

K

G

R

R

L

L

Q

Q

A

Q

V

V

V

C

K

Q

R

Y

A

A

Y

W

E

E

N

H

V

A

P

P

Y

F

Y

Y

R

R

K

R

R

K

F

W

D

E

E

E

A

A

G

G

V

F

K

Q

P

P

E

S

D

Q

I

L

K

K

T

S

L

L

K

E

D

D

I

F

E

E

-

A

K

R

L

V

P

P

F

V

T

V

T

K

K

K

T

T

D

D

L

L

R

R

D

E

A

S

Y

Q

-

A

-

A

-

H

-

P

P

P

F

F

G

G

M

D

F

Y

-

V

A

C

V

V

P

P

E

N

D

S

D

E

I

I

V

F

E

H

V

V

H

H

T

G

T

T

S

S

G

G

T

T

T

T

G

G

K

R

P

P

T

T

V

A

S

F

G

G

Y

I

T

G

V

R

N

A

D

D

L

W

D

R

L

A

W

I

G

A

E

N

V

A

I

H

A

A

R

R

A

I

L

M

G

W

M

G

A

M

G

G

A

I

G

R

K

P

K

G

D

D

I

L

I

V

Q

C

N

V

A

A

Y

A

G

V

Y

F

G

S

L

L

F
x
Y

T

M

G

G

G

S

M

W

G

G

V

A

H

L

Y

A

G

G

A

A

Q

E

K

R

I

L

G

R

A

A

T

K

V

A

V

F

P

P

I

F

S

G

A
|
A

G
|
G

N

A

-
x
P

-

G

-

M

T
x
S

M

A

R

R

Q

L

L

V

E

Q

I

W

M

L

E

D

D

T

F

M

G

K

S

P

T

A

V

A

L

F

T
x
Y

C
x
G

T
|
T

P

P

S

S

Y
|
Y

A

A

L

I

Y
x
H

L

L

G

A

E

E

M

V

A

A

E

R

R

E

E

E

G

K

I

L

S

N

R

P

E

R

S

N

I

F

K

G

L

L

K

K

A

C

G

L

V

F

F

F

G
x
S

A
x
G

E
|
E

-
x
P

-

G

M

A

W

S

T

V

E

P

E

G

M

V

R

K

N

D

E

R

I

I

E

E

K

E

R

A

L

Y

N

G

L

A

T

K

A

V

L

Y

N

D

I
x
C

Y
x
G

G
x
S

L
x
M

T
x
A

E
|
E

V

-

I

M

G

S

P

P

-

F

-

M

G

N

V

V

A

A

M

G

E

T

C

E

E

Q

D

S

K

N

N

D

G

G

L

M

H

L

I

C

S

W

D

Q

D

D

H

I

F

I

Y

Y

P

T

E

E

I

V

V

C

D

D

S

P

K

A

T

N

L

M

E

R

T

R

L

V

P

P

E

Y

G

G

E

Q

K

R

G

G

E

T

L

P

V

V

L

Y

T

T

T

H

L

L

T

E

R

R

E

T

G

S

M

Q

P

P

V

M

I

I

R

R

F

L

R

L

T

S

K

G

D
|
D

V

L

T

T

A

L

L

W

R

T

K

N

G

D

K

E

C

N

S

P

C

C

G

G

R

R

T

T

L

Y

I

P

K

R

M

L

D

P

R

Q

-

G

I
|
I

T

F

G

G

R

R

T

I

D

D

D

D

M

M

L

F

K

T

I

I

R
|
R

G
|
G

V

E

I
x
N

V

I

F

Y

P

P

S

S

Q

E

I

I

E

D

K

A

A

A

L

L

L

N

K

Q

I

M

E

S

D

G

L

Y

E

G

P

G

H

E

Y

H

Q

R

I

I

V

V

V

I

T

T

R

R

P

E

Q

S

H

A

L
x
M

D

D

E

E

L

L

E

L

V

L

Q

R

V

V

E

E

T

P

S

S

E

E

K

S

F

V

F

H

S

A

D

A

E

G

V

A

K

A

H

A

V

L

E

E

E

T

V

F

K

R

K

T

M

E

I

A

E

S

D

H

R

R

I

V

H

Q

S

T

E

V

I

L

G

G

L
x
V

R
|
R

V

A

N

K

V

V

T

E

L

L

V

V

E

A

P

P

Q

N

S

S

L

I

P

A

R

R

S

T

E

D

G

-

K

K

A

A

V

R

R

R

V

V

I

I

D

D

K

D

R

R

E

E

I

V

binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] 2-methyl-2-oxidanyl-propanoate: S241 (≠ G210), G242 (≠ A211), E243 (= E212), P244 (vs. gap), G267 (≠ Y234), S268 (≠ G235), M269 (≠ L236), A270 (≠ T237), D335 (= D301), I357 (= I322), N371 (≠ I336)
binding adenosine monophosphate: G242 (≠ A211), E243 (= E212), P244 (vs. gap), C266 (≠ I233), G267 (≠ Y234), S268 (≠ G235), A270 (≠ T237), E271 (= E238), D335 (= D301), N371 (≠ I336)
binding coenzyme a: Y166 (≠ F138), A188 (= A160), G189 (= G161), P191 (vs. gap), S194 (≠ T163), Y210 (≠ T179), G211 (≠ C180), T212 (= T181), Y215 (= Y184), H218 (≠ Y187), R368 (= R333), G369 (= G334), M401 (≠ L366), V439 (≠ L404), R440 (= R405)

6hdyA Crystal structure of 2-hydroxyisobutyryl-coa ligase (hcl) in the postadenylation state in complex with s3-hb-amp (see paper)
31% identity, 100% coverage: 3:432/432 of query aligns to 25:463/474 of 6hdyA

query
sites
6hdyA

W

W

N

F

K

P

E

R

A

R

E

E

C

T

M

M

S

P

E

A

N

A

D

E

K

R

E

D

A

K

L

A

Q

I

L

L

K

G

R

R

L

L

Q

Q

A

Q

V

V

V

C

K

Q

R

Y

A

A

Y

W

E

E

N

H

V

A

P

P

Y

F

Y

Y

R

R

K

R

R

K

F

W

D

E

E

E

A

A

G

G

V

F

K

Q

P

P

E

S

D

Q

I

L

K

K

T

S

L

L

K

E

D

D

I

F

E

E

-

A

K

R

L

V

P

P

F

V

T

V

T

K

K

K

T

T

D

D

L

L

R

R

D

E

A

S

Y

Q

-

A

-

A

-

H

-

P

P

P

F

F

G

G

M

D

F

Y

-

V

A

C

V

V

P

P

E

N

D

S

D

E

I

I

V

F

E

H

V

V

H

H

T

G

T

T

S

S

G

-

T

-

T

-

G

-

K

R

P

P

T

T

V

A

S

F

G

G

Y

I

T

G

V

R

N

A

D

D

L

W

D

R

L

A

W

I

G

A

E

N

V

A

I

H

A

A

R

R

A

I

L

M

G

W

M

G

A

M

G

G

A

I

G

R

K

P

K

G

D

D

I

L

I

V

Q

C

N

V

A

A

Y

A

G

V

Y

F

G

S

L

L

F
x
Y

T

M

G
|
G

G

S

M

W

G

G

V

A

H

L

Y

A

G

G

A

A

Q

E

K

R

I

L

G

R

A

A

T

K

V

A

V

F

P

P

I

F

S

G

A

A

G

G

N

A

-

P

-

G

-

M

T

S

M

A

R

R

Q

L

L

V

E

Q

I

W

M

L

E

D

D

T

F

M

G

K

S

P

T

A

V

A

L

F

T

Y

C

G

T

T

P

P

S

S

Y

Y

A

A

L

I

Y

H

L

L

G

A

E

E

M

V

A

A

E

R

R

E

E

E

G

K

I

L

S

N

R

P

E

R

S

N

I

F

K

G

L

L

K

K

A

C

G

L

V

F

F

F

G
x
S

A
x
G

E
|
E

-
x
P

-

G

M

A

W

S

T

V

E

P

E

G

M

V

R

K

N

D

E

R

I

I

E

E

K

E

R

A

L

Y

N

G

L

A

T

K

A

V

L

Y

N

D

I
x
C

Y
x
G

G
x
S

L
x
M

T
x
A

E

E

V

-

I

M

G

S

P

P

-
x
F

-

M

G

N

V

V

A

A

M

G

E

T

C

E

E

Q

D

S

K

N

N

D

G

G

L

M

H

L

I

C

S

W

D

Q

D

D

H

I

F

I

Y

Y

P

T

E

E

I

V

V

C

D

D

S

P

K

A

T

N

L

M

E

R

T

R

L

V

P

P

E

Y

G

G

E

Q

K

R

G

G

E

T

L

P

V

V

L

Y

T

T

T

H

L

L

T

E

R

R

E

T

G

S

M

Q

P

P

V

M

I

I

R

R

F

L

R

L

T

S

K

G

D
|
D

V

L

T

T

A

L

L

W

R

T

K

N

G

D

K

E

C

N

S

P

C

C

G

G

R

R

T

T

L

Y

I

P

K

R

M

L

D

P

R

Q

-

G

I
|
I

T

F

G

G

R
|
R

T

I

D

D

D

D

M

M

L

F

K

T

I

I

R

R

G

G

V

E

I

N

V

I

F

Y

P

P

S

S

Q

E

I

I

E

D

K

A

A

A

L

L

L

N

K

Q

I

M

E

S

D

G

L

Y

E

G

P

G

H

E

Y

H

Q

R

I

I

V

V

V

I

T

T

R

R

P

E

Q

S

H

A

L

M

D

D

E

E

L

L

E

L

V

L

Q

R

V

V

E

E

T

P

S

S

E

E

K

S

F

V

F

H

S

A

D

A

E

G

V

A

K

A

H

A

V

L

E

E

E

T

V

F

K

R

K

T

M

E

I

A

E

S

D

H

R

R

I

V

H

Q

S

T

E

V

I

L

G

G

L

V

R

R

V

A

N

K

V

V

T

E

L

L

V

V

E

A

P

P

Q

N

S

S

L

I

P

A

R

R

S

T

E

D

G

F

K
|
K

A

A

V

R

R

R

V

V

I

I

D

D

K

D

R

R

E

E

I

V

binding (3s)-3-hydroxybutanoic acid: Y162 (≠ F138), S237 (≠ G210), G263 (≠ Y234), S264 (≠ G235), M265 (≠ L236), A266 (≠ T237), F271 (vs. gap)
binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] (3~{S})-3-oxidanylbutanoate: Y162 (≠ F138), G164 (= G140), S237 (≠ G210), G238 (≠ A211), E239 (= E212), P240 (vs. gap), C262 (≠ I233), G263 (≠ Y234), S264 (≠ G235), A266 (≠ T237), F271 (vs. gap), D331 (= D301), I353 (= I322), R356 (= R325), K453 (= K422)

6hdxA Crystal structure of 2-hydroxyisobutyryl-coa ligase (hcl) in the postadenylation state in complex with r3-hib-amp (see paper)
31% identity, 100% coverage: 3:432/432 of query aligns to 25:463/474 of 6hdxA

query
sites
6hdxA

W

W

N

F

K

P

E

R

A

R

E

E

C

T

M

M

S

P

E

A

N

A

D

E

K

R

E

D

A

K

L

A

Q

I

L

L

K

G

R

R

L

L

Q

Q

A

Q

V

V

V

C

K

Q

R

Y

A

A

Y

W

E

E

N

H

V

A

P

P

Y

F

Y

Y

R

R

K

R

R

K

F

W

D

E

E

E

A

A

G

G

V

F

K

Q

P

P

E

S

D

Q

I

L

K

K

T

S

L

L

K

E

D

D

I

F

E

E

-

A

K

R

L

V

P

P

F

V

T

V

T

K

K

K

T

T

D

D

L

L

R

R

D

E

A

S

Y

Q

-

A

-

A

-

H

-

P

P

P

F

F

G

G

M

D

F

Y

-

V

A

C

V

V

P

P

E

N

D

S

D

E

I

I

V

F

E

H

V

V

H

H

T

G

T

T

S

S

G

-

T

-

T

-

G

-

K

R

P

P

T

T

V

A

S

F

G

G

Y

I

T

G

V

R

N

A

D

D

L

W

D

R

L

A

W

I

G

A

E

N

V

A

I

H

A

A

R

R

A

I

L

M

G

W

M

G

A

M

G

G

A

I

G

R

K

P

K

G

D

D

I

L

I

V

Q

C

N

V

A

A

Y

A

G

V

Y

F

G

S

L

L

F
x
Y

T

M

G
|
G

G

S

M

W

G

G

V

A

H

L

Y

A

G

G

A

A

Q

E

K

R

I

L

G

R

A

A

T

K

V

A

V

F

P

P

I

F

S

G

A

A

G

G

N

A

-

P

-

G

-

M

T

S

M

A

R

R

Q

L

L

V

E

Q

I

W

M

L

E

D

D

T

F

M

G

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T

A

V

A

L

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Y

C

G

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T

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P

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Y

Y

A

A

L

I

Y

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L

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A

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E

M

V

A

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E

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I

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R

P

E

R

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I

F

K

G

L

L

K

K

A

C

G

L

V

F

F

F

G
x
S

A
x
G

E
|
E

-
x
P

-

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M

A

W

S

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V

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P

E

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M

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D

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I

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K

E

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A

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A

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A

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L

Y

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D

I
x
C

Y
x
G

G
x
S

L

M

T
x
A

E

E

V

-

I

M

G

S

P

P

-
x
F

-

M

G

N

V

V

A

A

M

G

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T

C

E

E

Q

D

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K

N

N

D

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G

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D

Q

D

D

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|
D

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N

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D

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N

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C

C

G

G

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R

T

T

L

Y

I

P

K

R

M

L

D

P

R

Q

-

G

I
|
I

T

F

G

G

R
|
R

T

I

D

D

D

D

M

M

L

F

K

T

I

I

R

R

G

G

V

E

I

N

V

I

F

Y

P

P

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S

Q

E

I

I

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D

K

A

A

A

L

L

L

N

K

Q

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Y

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G

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G

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V

V

I

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R

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A

L

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D

D

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L

L

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L

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V

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S

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V

F

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S

A

D

A

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V

A

K

A

H

A

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L

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F

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K

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A

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H

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R

I

V

H

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I

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G

G

L

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R

R

V

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N

K

V

V

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L

L

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V

E

A

P

P

Q

N

S

S

L

I

P

A

R

R

S

T

E

D

G

F

K
|
K

A

A

V

R

R

R

V

V

I

I

D

D

K

D

R

R

E

E

I

V

binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] (2~{R})-2-methyl-3-oxidanyl-propanoate: Y162 (≠ F138), G164 (= G140), S237 (≠ G210), G238 (≠ A211), E239 (= E212), P240 (vs. gap), C262 (≠ I233), G263 (≠ Y234), S264 (≠ G235), A266 (≠ T237), F271 (vs. gap), D331 (= D301), I353 (= I322), R356 (= R325), K453 (= K422)
binding (2r)-3-hydroxy-2-methylpropanoic acid: Y162 (≠ F138), G164 (= G140), S237 (≠ G210), G263 (≠ Y234), S264 (≠ G235), A266 (≠ T237), F271 (vs. gap)

6siyA Paak family amp-ligase with amp and substrate (see paper)
28% identity, 97% coverage: 3:421/432 of query aligns to 8:406/433 of 6siyA

query
sites
6siyA

W

W

N

S

K

S

E

P

A

A

E

E

C

L

M

A

S

E

E

-

N

-

D

-

K

-

E

-

A

-

L

L

Q

Q

L

R

K

S

R

Q

L

L

Q

P

A

R

V

V

V

L

K

A

R

Q

A

A

Y

L

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N

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-

P

P

Y

F

Y

Y

R

A

K

A

R

R

F

Y

D

R

E

-

A

-

G

G

V

T

K

T

P

P

E

P

D

-

I

-

K

R

T

T

L

A

K

D

D

D

I

F

E

A

K

G

L

V

P

E

F

V

T

T

T

A

K

K

T

Q

D

D

L

L

R

R

D

D

A

Q

Y

Y

P

P

F

F

G

G

M

M

F

L

A

A

V

V

P

G

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x
R

D
x
E

D

H

I
x
L

V

A

E

T

V

Y

H

H

T

E

T

S

S
|
S

G

G

T

T

T

A

G

G

K

E

P

P

T

T

V

A

S

S

G

Y

Y

Y

T

T

V

E

N

E

D

D

L

W

D

T

L

D

W

L

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A

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R

I

F

A

A

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R

A

K

L

-

G

-

M

W

A

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G

G

A

I

G

H

K
x
P

K

S

D

D

I

T

I

F

Q

L

N

V

A

R

Y
x
T

G
x
P

Y

Y

G

G

L

L

F

V

T

I

G
x
T

G

G

M

H

G
x
L

V

A

H

Q

Y

A

G

A

A

G

Q

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K

L

I

R

G
|
G

A
|
A

T
|
T

V

V

V

V

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P

I

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S

D

A

A

G
x
R

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A

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Y

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A

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M

L

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A

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A

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A

A

A

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A

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A

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G

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K

H

L

L

K

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A

A

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M

V

F

F

T

G

A

A
|
A

E
|
E

M
x
P

W

L

T

T

E

E

E

V

M

R

R

R

N

R

E

R

I

L

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K

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I

L

W

-

G

N

G

L

I

T

P

A

V

L

V

N

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I
x
E

Y
|
Y

G
|
G

L
x
S

T
|
T

E

E

V

T

I

G

G

-

P

-

G

T

V

I

A

A

M

G

E

Q

C

C

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P

D

E

K

G

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R

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L

M

H

H

I

L

S

W

D

A

D

D

H

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A

Y

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F

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I

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V

Y

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D

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T

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L

L

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S

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A

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G

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A

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M

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P

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L

I

L

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R

F

Y

R

N

T

L

K

A

D
|
D

V

D

T

V

A

E

L

V

R

S

K

T

G

D

K

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C

C

S

G

C

C

G

G

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-

T

W

L

L

I

L

K

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M

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x
V

T

L

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G

R

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G

L

H

K

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I

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A

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Q

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E

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A

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L

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L

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L

L

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A

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Y

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A

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V

K

K

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L

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A

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A

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-

K

-

M

-

I

-

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-

D

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-

I

L

H

D

S

R

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E

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L

G

G

L

V

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V

H

N

R

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T