SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_048081385.1 NCBI__GCF_000745485.1:WP_048081385.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

8a6tC Cryo-em structure of the electron bifurcating fe-fe hydrogenase hydabc complex from thermoanaerobacter kivui in the reduced state (see paper)
51% identity, 92% coverage: 3:146/156 of query aligns to 5:148/151 of 8a6tC

query
sites
8a6tC

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binding fe2/s2 (inorganic) cluster: C77 (= C75), G79 (= G77), A81 (= A79), C82 (= C80), C118 (= C116), L119 (= L117), C122 (= C120)

O52681 Bifurcating [FeFe] hydrogenase gamma subunit; Hydrogenase (NAD(+), ferredoxin) gamma subunit; Iron-hydrogenase gamma subunit; EC 1.12.1.4 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
48% identity, 98% coverage: 3:155/156 of query aligns to 6:158/161 of O52681

query
sites
O52681

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D79 (≠ T76) mutation to P: Increase (30 mV) in the redox potential.
G80 (= G77) mutation to P: Large decrease (149 mV) in the redox potential. Causes a change to a [4Fe-4S] cluster protein.
T81 (= T78) mutation to P: Small increase (11 mV) in the redox potential.
A82 (= A79) mutation to P: Decrease (46 mV) in the redox potential.
L120 (= L117) mutation to P: Large decrease (65 mV) in the redox potential.
G121 (= G118) mutation to P: Decrease (22 mV) in the redox potential.
A122 (≠ C119) mutation to P: Large decrease (132 mV) in the redox potential.
V128 (≠ C125) mutation V->A,C,H,Y: Small increase (about 2-14 mV) in the redox potential.; mutation V->D,G,R,S,T,W: Increase (about 23-33 mV) in the redox potential.; mutation V->E,K,L,M: Decrease (about 37-50 mV) in the redox potential.; mutation V->F,I,P,Q: Small decrease (about 4-20 mV) in the redox potential.; mutation to N: Large increase (85 mV) in the redox potential.

7t2rC Structure of electron bifurcating ni-fe hydrogenase complex hydabcsl in fmn-free apo state (see paper)
47% identity, 93% coverage: 3:147/156 of query aligns to 6:150/152 of 7t2rC

query
sites
7t2rC

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binding fe2/s2 (inorganic) cluster: C78 (= C75), C83 (= C80), C119 (= C116), L120 (= L117), G121 (= G118), A122 (≠ C119), C123 (= C120), V128 (≠ C125)

7p5hC Tmhydabc- d2 map (see paper)
48% identity, 98% coverage: 3:155/156 of query aligns to 3:155/156 of 7p5hC

query
sites
7p5hC

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binding fe2/s2 (inorganic) cluster: C75 (= C75), G77 (= G77), A79 (= A79), C80 (= C80), C116 (= C116), L117 (= L117), A119 (≠ C119), C120 (= C120)

8oh5A Cryo-em structure of the electron bifurcating transhydrogenase stnabc complex from sporomusa ovata (state 2) (see paper)
42% identity, 91% coverage: 5:146/156 of query aligns to 6:147/150 of 8oh5A

query
sites
8oh5A

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binding fe2/s2 (inorganic) cluster: C76 (= C75), C81 (= C80), C117 (= C116), L118 (= L117), G119 (= G118), C120 (= C119), C121 (= C120)

8a5eC Cryo-em structure of the electron bifurcating fe-fe hydrogenase hydabc complex from acetobacterium woodii in the reduced state (see paper)
37% identity, 93% coverage: 3:147/156 of query aligns to 11:155/156 of 8a5eC

query
sites
8a5eC

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binding fe2/s2 (inorganic) cluster: C83 (= C75), G85 (= G77), A87 (= A79), C88 (= C80), R123 (≠ G115), C124 (= C116), C128 (= C120)

6tg9G Cryo-em structure of nadh reduced form of NAD+-dependent formate dehydrogenase from rhodobacter capsulatus (see paper)
35% identity, 88% coverage: 4:141/156 of query aligns to 5:140/148 of 6tg9G

query
sites
6tg9G

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binding fe2/s2 (inorganic) cluster: C76 (= C75), A78 (≠ G77), C81 (= C80), C115 (= C116), L116 (= L117), L118 (≠ C119), C119 (= C120)

8eswV2 NADH dehydrogenase (Ubiquinone) 24 kDa subunit, isoform A (see paper)
34% identity, 94% coverage: 4:150/156 of query aligns to 27:175/214 of 8eswV2

query
sites
8eswV2

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H

W

V

L

Q

R

G

G

A

S

P

D

Q

D

I

I

L

L

A

E

G

T

I

C

E

K

R

K

H

Q

L

L

G

G

I

I

K

G

E

V

G

G

E

D

V

T

T

T

T

K

D

D

L

R

E

K

Y

F

S

T

L

I

E

S

S

E

V

V

G

E

C
|
C

L
|
L

G
|
G

C

A

C
|
C

A

V

L

N

A

A

P

P

C

M

A

V

M

A

V

I

N

N

D

D

E

D

V

Y

E

Y

S

E

H

D

I

L

S

T

L

S

K

K

N

D

I

M

K

Q

R

D

L

I

F

L

K

N

R

D

K

L

K

K

S

A

binding fe2/s2 (inorganic) cluster: C100 (= C75), C105 (= C80), C141 (= C116), L142 (= L117), G143 (= G118), C145 (= C120)
binding : R27 (≠ K4), A30 (≠ E7), E37 (vs. gap), R53 (≠ S28)

8b9zE Drosophila melanogaster complex i in the active state (dm1) (see paper)
34% identity, 94% coverage: 4:150/156 of query aligns to 27:175/214 of 8b9zE

query
sites
8b9zE

K

R

L

V

N

E

E

A

I

I

L

L

S

S

S

I

Y

Y

K

P

-

E

-

G

G

H

E

K

K

R

S

G

E

A

L

M

I

I

P

P

I

L

L

L

Q

D

D

L

I

A

Q

Q

S

R

N

Q

Y

Y

G

G

Y

W

L

L

P

P

E

I

D

S

I

A

I

M

V

H

E

K

L

V

S

A

N

E

F

I

L

L

K

Q

M

L

P

P

E

N

S

M

E

R

I

V

Y

Y

G

E

V

V

A

A

T

T

F

F

Y

Y

S

T

Q

M

F

F

R

M

F

R

T

K

P

P

V

T

G

G

K

K

K

Y

H

H

I

I

M

Q

V

V

C
|
C

T

T

G
x
T

T
|
T

A
x
P

C
|
C

H

W

V

L

Q

R

G

G

A

S

P

D

Q

D

I

I

L

L

A

E

G

T

I

C

E

K

R

K

H

Q

L

L

G

G

I

I

K

G

E

V

G

G

E

D

V

T

T

T

T

K

D

D

L

R

E

K

Y

F

S

T

L

I

E

S

S

E

V

V

G

E

C
|
C

L
|
L

G
|
G

C
x
A

C
|
C

A

V

L

N

A

A

P

P

C

M

A

V

M

A

V

I

N

N

D

D

E

D

V

Y

E

Y

S

E

H

D

I

L

S

T

L

S

K

K

N

D

I

M

K

Q

R

D

L

I

F

L

K

N

R

D

K

L

K

K

S

A

binding fe2/s2 (inorganic) cluster: C100 (= C75), T102 (≠ G77), T103 (= T78), P104 (≠ A79), C105 (= C80), C141 (= C116), L142 (= L117), G143 (= G118), A144 (≠ C119), C145 (= C120)

5gupE structure of mammalian respiratory supercomplex I1III2IV1 (see paper)
36% identity, 94% coverage: 4:150/156 of query aligns to 40:188/197 of 5gupE

query
sites
5gupE

K

R

L

I

N

E

E

A

I

I

L

V

S

K

S

N

Y

Y

-

P

K

E

G

G

E

H

K

K

S

A

E

A

-

A

L

V

I

L

P

P

I

V

L

L

Q

D

D

L

I

A

Q

Q

S

R

N

Q

Y

N

G

G

Y

W

L

L

P

P

E

I

D

S

I

A

I

M

V

N

E

K

L

V

S

A

N

E

F

I

L

L

K

Q

M

V

P

P

E

P

S

M

E

R

I

V

Y

Y

G

E

V

V

A

A

T

T

F

F

Y

Y

S

T

Q

M

F

Y

R

N

F

R

T

K

P

P

V

V

G

G

K

K

K

Y

H

H

I

I

M

Q

V

V

C
|
C

T

T

G
x
T

T

T

A
x
P

C
|
C

H

M

V

L

Q

R

G

N

A

S

P

D

Q

S

I

I

L

L

A

E

G

A

I

I

E

Q

R

K

H

K

L

L

G

G

I

I

K

K

E

V

G

G

E

E

V

T

T

T

T

P

D

D

L

K

E

L

Y

F

S

T

L

L

E

I

S

E

V

V

G

E

C
|
C

L
|
L

G
|
G

C
x
A

C
|
C

A

V

L

N

A

A

P

P

C
x
M

A

V

M

Q

V

I

N

N

D

D

E

N

V

Y

E

Y

S

E

H

D

I

L

S

T

L

P

K

K

N

D

I

I

K

E

R

E

L

I

F

I

K

D

R

E

K

L

K

K

S

A

binding fe2/s2 (inorganic) cluster: C113 (= C75), T115 (≠ G77), P117 (≠ A79), C118 (= C80), C154 (= C116), L155 (= L117), G156 (= G118), A157 (≠ C119), C158 (= C120), M163 (≠ C125)

7dgq9 NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (see paper)
36% identity, 94% coverage: 4:150/156 of query aligns to 23:171/207 of 7dgq9

query
sites
7dgq9

K
x
R

L

I

N

E

E

A

I

I

L

V

S
x
K

S

N

Y

Y

-
x
P

K

E

G

G

E
x
H

K

K

S

A

E

A

-

A

L

V

I

L

P

P

I

V

L

L

Q

D

D

L

I

A

Q

Q

S
x
R

N

Q

Y

N

G

G

Y

W

L

L

P

P

E

I

D

S

I

A

I

M

V

N

E

K

L

V

S

A

N

E

F

I

L

L

K

Q

M

V

P

P

E

P

S

M

E

R

I

V

Y

Y

G

E

V

V

A

A

T

T

F

F

Y

Y

S

T

Q

M

F

Y

R

N

F

R

T

K

P

P

V

V

G

G

K

K

K

Y

H

H

I

I

M

Q

V

V

C
|
C

T

T

G
x
T

T

T

A

P

C
|
C

H

M

V

L

Q

R

G

N

A

S

P

D

Q

S

I

I

L

L

A

E

G

A

I

I

E

Q

R

K

H

K

L

L

G

G

I

I

K

K

E

V

G

G

E

E

V

T

T

T

T

P

D

D

L

K

E

L

Y

F

S

T

L

L

E

I

S

E

V

V

G

E

C
|
C

L
|
L

G

G

C
x
A

C
|
C

A

V

L

N

A

A

P

P

C

M

A

V

M

Q

V

I

N

N

D

D

E

N

V

Y

E

Y

S

E

H

D

I

L

S

T

L

P

K

K

N

D

I

I

K

E

R

E

L

I

F

I

K

D

R

E

K

L

K

K

S

A

binding fe2/s2 (inorganic) cluster: C96 (= C75), T98 (≠ G77), C101 (= C80), C137 (= C116), L138 (= L117), A140 (≠ C119), C141 (= C120)
binding : R23 (≠ K4), K29 (≠ S10), P32 (vs. gap), H35 (≠ E15), R49 (≠ S28)

Sites not aligning to the query:

binding : 20

P19404 NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial; NDUFV2; NADH-ubiquinone oxidoreductase 24 kDa subunit; EC 7.1.1.2 from Homo sapiens (Human) (see 2 papers)
36% identity, 94% coverage: 4:150/156 of query aligns to 62:210/249 of P19404

query
sites
P19404

K

R

L

I

N

E

E

A

I

I

L

V

S

K

S

N

Y

Y

-

P

K

E

G

G

E

H

K

K

S

A

E

A

-

A

L

V

I

L

P

P

I

V

L

L

Q

D

D

L

I

A

Q

Q

S

R

N

Q

Y

N

G

G

Y

W

L

L

P

P

E

I

D

S

I

A

I

M

V

N

E

K

L

V

S

A

N

E

F

V

L

L

K

Q

M

V

P

P

E

P

S

M

E

R

I

V

Y

Y

G

E

V

V

A

A

T

T

F

F

Y

Y

S

T

Q

M

F

Y

R

N

F

R

T

K

P

P

V

V

G

G

K

K

K

Y

H

H

I

I

M

Q

V

V

C

C

T

T

G

T

T

T

A

P

C

C

H

M

V

L

Q

R

G

N

A

S

P

D

Q

S

I

I

L

L

A

E

G

A

I

I

E

Q

R

K

H

K

L

L

G

G

I

I

K

K

E

V

G

G

E

E

V

T

T

T

T

P

D

D

L

K

E

L

Y

F

S

T

L

L

E

I

S

E

V

V

G

E

C

C

L

L

G

G

C

A

C

C

A

V

L

N

A

A

P

P

C

M

A

V

M

Q

V

I

N

N

D

D

E

N

V

Y

E
x
Y

S

E

H

D

I

L

S

T

L

A

K

K

N

D

I

I

K

E

R

E

L

I

F

I

K

D

R

E

K

L

K

K

S

A

Y193 (≠ E133) modified: Phosphotyrosine; by SRC

Sites not aligning to the query:

29 V → A: in dbSNP:rs906807

P04394 NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial; NADH dehydrogenase subunit II; NADH-ubiquinone oxidoreductase 24 kDa subunit; EC 7.1.1.2 from Bos taurus (Bovine) (see 2 papers)
36% identity, 94% coverage: 4:150/156 of query aligns to 62:210/249 of P04394

query
sites
P04394

K

R

L

I

N

E

E

A

I

I

L

V

S

K

S

N

Y

Y

-

P

K

E

G

G

E

H

K

K

S

A

E

A

-

A

L

V

I

L

P

P

I

V

L

L

Q

D

D

L

I

A

Q

Q

S

R

N

Q

Y

N

G

G

Y

W

L

L

P

P

E

I

D

S

I

A

I

M

V

N

E

K

L

V

S

A

N

E

F

I

L

L

K

Q

M

V

P

P

E

P

S

M

E

R

I

V

Y

Y

G

E

V

V

A

A

T

T

F

F

Y

Y

S

T

Q

M

F

Y

R

N

F

R

T

K

P

P

V

V

G

G

K

K

K

Y

H

H

I

I

M

Q

V

V

C
|
C

T

T

G

T

T

T

A

P

C
|
C

H

M

V

L

Q

R

G

N

A

S

P

D

Q

S

I

I

L

L

A

E

G

A

I

I

E

Q

R

K

H

K

L

L

G

G

I

I

K

K

E

V

G

G

E

E

V

T

T

T

T

P

D

D

L

K

E

L

Y

F

S

T

L

L

E

I

S

E

V

V

G

E

C
|
C

L

L

G

G

C

A

C
|
C

A

V

L

N

A

A

P

P

C

M

A

V

M

Q

V

I

N

N

D

D

E

N

V

Y

E

Y

S

E

H

D

I

L

S

T

L

P

K

K

N

D

I

I

K

E

R

E

L

I

F

I

K

D

R

E

K

L

K

K

S

A

C135 (= C75) binding [2Fe-2S] cluster
C140 (= C80) binding [2Fe-2S] cluster
C176 (= C116) binding [2Fe-2S] cluster
C180 (= C120) binding [2Fe-2S] cluster

Sites not aligning to the query:

1:32 modified: transit peptide, Mitochondrion

7v2cO Active state complex i from q10 dataset (see paper)
36% identity, 94% coverage: 4:150/156 of query aligns to 30:178/217 of 7v2cO

query
sites
7v2cO

K

R

L

I

N

E

E

A

I

I

L

V

S

K

S

N

Y

Y

-

P

K

E

G

G

E

H

K

K

S

A

E

A

-

A

L

V

I

L

P

P

I

V

L

L

Q

D

D

L

I

A

Q

Q

S

R

N

Q

Y

N

G

G

Y

W

L

L

P

P

E

I

D

S

I

A

I

M

V

N

E

K

L

V

S

A

N

E

F

I

L

L

K

Q

M

V

P

P

E

P

S

M

E

R

I

V

Y

Y

G

E

V

V

A

A

T

T

F

F

Y

Y

S

T

Q

M

F

Y

R

N

F

R

T

K

P

P

V

V

G

G

K

K

K

Y

H

H

I

I

M

Q

V

V

C
|
C

T

T

G

T

T

T

A
x
P

C
|
C

H

M

V

L

Q

R

G

N

A

S

P

D

Q

S

I

I

L

L

A

E

G

A

I

I

E

Q

R

K

H

K

L

L

G

G

I

I

K

K

E

V

G

G

E

E

V

T

T

T

T

P

D

D

L

K

E

L

Y

F

S

T

L

L

E

I

S

E

V

V

G

E

C
|
C

L
|
L

G
|
G

C
x
A

C
|
C

A

V

L

N

A

A

P

P

C

M

A

V

M

Q

V

I

N

N

D

D

E

N

V

Y

E

Y

S

E

H

D

I

L

S

T

L

P

K

K

N

D

I

I

K

E

R

E

L

I

F

I

K

D

R

E

K

L

K

K

S

A

binding fe2/s2 (inorganic) cluster: C103 (= C75), P107 (≠ A79), C108 (= C80), C144 (= C116), L145 (= L117), G146 (= G118), A147 (≠ C119), C148 (= C120)

Q9D6J6 NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial; NADH-ubiquinone oxidoreductase 24 kDa subunit; EC 7.1.1.2 from Mus musculus (Mouse) (see paper)
36% identity, 87% coverage: 15:150/156 of query aligns to 74:209/248 of Q9D6J6

query
sites
Q9D6J6

E

Q

K

A

S

A

E

A

L

V

I

L

P

P

I

V

L

L

Q

D

D

L

I

A

Q

Q

S

R

N

Q

Y

N

G

G

Y

W

L

L

P

P

E

I

D

S

I

A

I

M

V

N

E

K

L

V

S

A

N

E

F

V

L

L

K

Q

M

V

P

P

E

P

S

M

E

R

I

V

Y

Y

G

E

V

V

A

A

T

T

F

F

Y

Y

S

T

Q

M

F

Y

R

N

F

R

T

K

P

P

V

V

G

G

K

K

K

Y

H

H

I

I

M

Q

V

V

C
|
C

T

T

G

T

T

T

A

P

C
|
C

H

M

V

L

Q

R

G

D

A

S

P

D

Q

S

I

I

L

L

A

E

G

T

I

L

E

Q

R

R

H

K

L

L

G

G

I

I

K

K

E

V

G

G

E

E

V

T

T

T

T

P

D

D

L

K

E

L

Y

F

S

T

L

L

E

I

S

E

V

V

G

E

C
|
C

L

L

G

G

C

A

C
|
C

A

V

L

N

A

A

P

P

C

M

A

V

M

Q

V

I

N

N

D

D

E

N

V

Y

E

Y

S

E

H

D

I

L

S

T

L

P

K

K

N

D

I

I

K

E

R

E

L

I

F

I

K

D

R

E

K

L

K

K

S

A

C134 (= C75) binding [2Fe-2S] cluster
C139 (= C80) binding [2Fe-2S] cluster
C175 (= C116) binding [2Fe-2S] cluster
C179 (= C120) binding [2Fe-2S] cluster

3iam2 Crystal structure of the hydrophilic domain of respiratory complex i from thermus thermophilus, reduced, 2 mol/asu, with bound nadh (see paper)
36% identity, 83% coverage: 2:131/156 of query aligns to 7:138/179 of 3iam2

query
sites
3iam2

E

Q

D

D

K

F

L

L

N

E

E

E

I

T

L

F

S

A

S

K

Y

Y

-

P

K

E

G

G

E

R

K

R

S

A

E

A

L

I

I

M

P

P

I

L

L

L

Q

R

D

R

I

V

Q

Q

S

Q

N

E

Y

E

G

G

Y

W

L

I

P

R

E

P

D

E

I

R

I

I

V

E

E

E

L

I

S

A

N

R

F

L

L

V

K

G

M

T

P

T

E

P

S

T

E

E

I

V

Y

M

G

G

V

V

A

A

T

S

F

F

Y

Y

S

S

Q

Y

F

Y

R

Q

F

F

T

V

P

P

V

T

G

G

K

K

K

Y

H

H

I

L

M

Q

V

V

C
|
C

T

A

G

T

T

L

A

S

C
|
C

H

K

V

L

Q

A

G

G

A

A

P

E

Q

E

I

L

L

W

A

D

G

Y

I

L

E

T

R

E

H

T

L

L

G

G

I

I

K

G

E

P

G

G

E

E

V

V

T

T

T

P

D

D

L

G

E

L

Y

F

S

S

L

V

E

Q

S

K

V

V

G

E

C
|
C

L

L

G

G

C

S

C
|
C

A

H

L

T

A

A

P

P

C

V

A

I

M

Q

V

V

N

N

D

D

E

E

binding fe2/s2 (inorganic) cluster: C82 (= C75), C87 (= C80), C123 (= C116), C127 (= C120)

3i9v2 Crystal structure of the hydrophilic domain of respiratory complex i from thermus thermophilus, oxidized, 2 mol/asu (see paper)
36% identity, 83% coverage: 2:131/156 of query aligns to 6:137/178 of 3i9v2

query
sites
3i9v2

E

Q

D

D

K

F

L

L

N

E

E

E

I

T

L

F

S

A

S

K

Y

Y

-

P

K

E

G

G

E

R

K

R

S

A

E

A

L

I

I

M

P

P

I

L

L

L

Q

R

D

R

I

V

Q

Q

S

Q

N

E

Y

E

G

G

Y

W

L

I

P

R

E

P

D

E

I

R

I

I

V

E

E

E

L

I

S

A

N

R

F

L

L

V

K

G

M

T

P

T

E

P

S

T

E

E

I

V

Y

M

G

G

V

V

A

A

T

S

F

F

Y

Y

S
|
S

Q

Y

F

Y

R

Q

F

F

T

V

P

P

V

T

G

G

K

K

K

Y

H

H

I

L

M

Q

V

V

C
|
C

T

A

G
x
T

T

L

A
x
S

C
|
C

H

K

V

L

Q

A

G

G

A

A

P

E

Q

E

I

L

L

W

A

D

G

Y

I

L

E

T

R

E

H

T

L

L

G

G

I

I

K

G

E

P

G

G

E

E

V

V

T

T

T

P

D

D

L

G

E

L

Y

F

S

S

L

V

E

Q

S

K

V
|
V

G
x
E

C
|
C

L
|
L

G
|
G

C
x
S

C
|
C

A

H

L

T

A

A

P

P

C

V

A

I

M

Q

V

V

N

N

D

D

E

E

binding fe2/s2 (inorganic) cluster: C81 (= C75), T83 (≠ G77), S85 (≠ A79), C86 (= C80), C122 (= C116), L123 (= L117), G124 (= G118), S125 (≠ C119), C126 (= C120)
binding manganese (ii) ion: S66 (= S60), V120 (= V114), E121 (≠ G115)

Q56221 NADH-quinone oxidoreductase subunit 2; NADH dehydrogenase I chain 2; NDH-1 subunit 2; EC 7.1.1.- from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see 2 papers)
36% identity, 83% coverage: 2:131/156 of query aligns to 8:139/181 of Q56221

query
sites
Q56221

E

Q

D

D

K

F

L

L

N

E

E

E

I

T

L

F

S

A

S

K

Y

Y

-

P

K

E

G

G

E

R

K

R

S

A

E

A

L

I

I

M

P

P

I

L

L

L

Q

R

D

R

I

V

Q

Q

S

Q

N

E

Y

E

G

G

Y

W

L

I

P

R

E

P

D

E

I

R

I

I

V

E

E

E

L

I

S

A

N

R

F

L

L

V

K

G

M

T

P

T

E

P

S

T

E

E

I

V

Y

M

G

G

V

V

A

A

T

S

F

F

Y

Y

S

S

Q

Y

F

Y

R

Q

F

F

T

V

P

P

V

T

G

G

K

K

K

Y

H

H

I

L

M

Q

V

V

C
|
C

T

A

G

T

T

L

A
x
S

C
|
C

H

K

V

L

Q

A

G

G

A

A

P

E

Q

E

I

L

L

W

A

D

G

Y

I

L

E

T

R

E

H

T

L

L

G

G

I

I

K

G

E

P

G

G

E

E

V

V

T

T

T

P

D

D

L

G

E

L

Y

F

S

S

L

V

E

Q

S

K

V

V

G

E

C
|
C

L

L

G

G

C

S

C
|
C

A

H

L

T

A

A

P

P

C

V

A

I

M

Q

V

V

N

N

D

D

E

E

C83 (= C75) binding [2Fe-2S] cluster
S87 (≠ A79) binding [2Fe-2S] cluster
C88 (= C80) binding [2Fe-2S] cluster
C124 (= C116) binding [2Fe-2S] cluster
C128 (= C120) binding [2Fe-2S] cluster

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
144 modified: Disulfide link with 172
172 modified: Disulfide link with 144

9fdkC Crystal structure of oxidized nuoef variant r66g(nuof) from aquifex aeolicus (see paper)
35% identity, 90% coverage: 1:141/156 of query aligns to 12:155/160 of 9fdkC

query
sites
9fdkC

M

L

E

K

D

T

K

K

L

L

N

Q

E

E

I

H

L

I

S

N

S

Y

Y

F

K

P

G

K

E

K

K

R

S

Q

E

A

L

I

I

L

P

L

I

C

L

L

Q

H

D

E

I

I

Q

Q

S

N

N

Y

Y

Y

G

G

Y

Y

L

I

P

P

E

P

D

E

I

S

I

L

V

K

E

P

L

L

S

A

N

D

F

M

L

L

K

E

M

L

P

P

E

L

S

N

E

H

I

V

Y

E

G

G

V

V

A

V

T

A

F

F

Y

Y

S

D

Q

M

F

F

R

D

F

R

T

E

P

D

V

K

G

A

K

K

K

Y

H

R

I

I

M

R

V

V

C
|
C

T

V

G

S

T

I

A
x
V

C
|
C

H

H

V

L

Q

M

G

G

A

T

P

N

Q

K

I

L

L

L

A

K

G

A

I

L

E

E

R

N

H

I

L

L

G

G

I

I

K

K

E

P

G

G

E

E

V

V

T

T

T

P

D

D

L

G

E

K

Y

F

S

K

L

I

E

V

S

P

V

V

G

Q

C
|
C

L
|
L

G

G

C
x
A

C
|
C

A

S

L

E

A

A

P

P

C

V

A

F

M

M

V

V

N

N

D

D

E

D

-

E

-

Y

-

K

V

F

E

E

S

S

H

E

I

V

S

Q

L

L

K

N

N

E

I

I

binding fe2/s2 (inorganic) cluster: C86 (= C75), V90 (≠ A79), C91 (= C80), C127 (= C116), L128 (= L117), A130 (≠ C119), C131 (= C120)

7zmbH Cryoem structure of mitochondrial complex i from chaetomium thermophilum (state 2) (see paper)
34% identity, 92% coverage: 2:145/156 of query aligns to 26:173/221 of 7zmbH

query
sites
7zmbH

E

E

D

K

K

I

L

I

N

E

E

Q

I

I

L

L

S

K

S

R

Y

Y

K

P

G

P

E

Q

-

Y

-

K

K

K

S

A

E

A

L

V

I

M

P

P

I

L

L

L

Q

D

D

L

I

G

Q

Q

S

R

N

Q

Y

H

G

G

Y

F

L

C

P

S

E

I

D

S

I

V

I

M

V

N

E

E

L

V

S

A

N

R

F

I

L

L

K

E

M

M

P

P

E

P

S

M

E

R

I

V

Y

Y

G

E

V

V

A

A

T

S

F

F

Y

Y

S

T

Q

M

F

Y

R

N

F

R

T

T

P

P

V

V

G

G

K

K

K

F

H

H

I

V

M

Q

V

A

C
|
C

T

T

G

T

T

T

A
x
P

C
|
C

H

Q

V

L

Q

G

-

G

-

C

G

G

A

S

P

D

Q

A

I

I

L

V

A

K

G

A

I

I

E

K

R

E

H

H

L

L

G

G

I

I

K

N

E

Q

G

G

E

E

V

T

T

T

T

P

D

D

L

G

E

L

Y

F

S

T

L

F

E

I

S

E

V

V

G

E

C
|
C

L

L

G

G

C
x
A

C
|
C

A

V

L

N

A

A

P

P

C

M

A

V

M

Q

V

I

N

N

D

D

E

D

V

Y

E

Y

S

E

H

D

I

L

S

T

L

P

K

E

N

T

I

I

K

K

R

Q

L

V

F

L

binding fe2/s2 (inorganic) cluster: C101 (= C75), P105 (≠ A79), C106 (= C80), C144 (= C116), A147 (≠ C119), C148 (= C120)

Query Sequence

>WP_048081385.1 NCBI__GCF_000745485.1:WP_048081385.1
MEDKLNEILSSYKGEKSELIPILQDIQSNYGYLPEDIIVELSNFLKMPESEIYGVATFYS
QFRFTPVGKKHIMVCTGTACHVQGAPQILAGIERHLGIKEGEVTTDLEYSLESVGCLGCC
ALAPCAMVNDEVESHISLKNIKRLFKRKKSKEKASN

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory