SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_048081429.1 NCBI__GCF_000746075.1:WP_048081429.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
32% identity, 87% coverage: 20:245/260 of query aligns to 26:252/265 of P07821

query
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P07821

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K50 (= K44) mutation to Q: Lack of activity.
D172 (= D166) mutation to E: Lack of activity.
E173 (= E167) mutation to A: Lack of activity.

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
32% identity, 87% coverage: 20:245/260 of query aligns to 17:240/241 of 4u00A

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binding adenosine-5'-diphosphate: V17 (≠ I20), S37 (≠ N40), G38 (= G41), S39 (≠ T42), G40 (= G43), K41 (= K44), S42 (= S45), T43 (= T46)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

5x40A Structure of a cbio dimer bound with amppcp (see paper)
31% identity, 95% coverage: 1:248/260 of query aligns to 1:253/280 of 5x40A

query
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binding phosphomethylphosphonic acid adenylate ester: F14 (≠ Y14), V18 (≠ E18), A20 (≠ I20), N40 (= N40), G41 (= G41), G43 (= G43), K44 (= K44), S45 (= S45), T46 (= T46), Q88 (= Q86), H139 (≠ T140), M140 (≠ E141), L141 (≠ I142), S142 (= S143), G144 (= G145), Q145 (≠ E146), Q166 (≠ E167), H198 (= H199)
binding magnesium ion: S45 (= S45), Q88 (= Q86)

6mjpA Lptb(e163q)fgc from vibrio cholerae (see paper)
33% identity, 90% coverage: 4:238/260 of query aligns to 2:233/240 of 6mjpA

query
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binding calcium ion: E111 (≠ G115), D115 (≠ C119)

7ahhC Opua inhibited inward-facing, sbd docked (see paper)
34% identity, 80% coverage: 23:230/260 of query aligns to 44:252/382 of 7ahhC

query
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7ahhC

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binding phosphoaminophosphonic acid-adenylate ester: G62 (= G41), G64 (= G43), K65 (= K44), D187 (= D166), E188 (= E167)

Sites not aligning to the query:

7aheC Opua inhibited inward facing (see paper)
34% identity, 80% coverage: 23:230/260 of query aligns to 44:252/382 of 7aheC

query
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7aheC

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K

N

E

D

D

L

L

A

L

K

Q

I

V

I

R

G

R

Y

K

I

T

P

M

Q

S

S

M

H

V

N

F

S

Q

T

N

F

F

A

G

F

L

S

F

V

P

F

H

D

R

V

T

V

I

L

L

M

E

G

N

R

T

A

E

P

Y

H

G

L

L

S

E

L

V

T

Q

S

N

V

V

P

P

G

K

E

E

K

E

D

R

C

R

K

K

I

R

A

A

E

E

E

K

A

A

L

L

K

D

S

N

L

A

G

N

I

L

S

L

H

D

L

F

T

K

D

D

K

Q

T

Y

Y

P

T

K

E

Q

I

L

S

S

G

G

E

M

R

Q

Q

Q

M

R

V

V

L

G

I

L

A

A

R

R

V

A

L

L

A

A

Q

N

Q

D

P

P

Q

E

I

I

L

L

L

M

D

D

E

E

P

A

T

F

S

S

H

A

L

L

D

D

F

P

G

L

N

I

Q

R

I

R

R

E

T

M

L

Q

D

D

V

E

I

L

N

L

K

E

L

L

-

Q

A

A

E

K

N

F

G

Q

L

K

S

T

V

I

I

I

M

F

T

V

S

S

H

H

F

D

P

L

D

N

H

E

A

A

F

L

L

R

S

I

S

G

N

D

K

R

V

I

A

A

I

I

M

M

N

K

G

D

G

G

T

K

I

I

M

M

E

Q

M

I

G

G

T

T

P

G

E

E

S

E

V

I

I

L

T

T

Sites not aligning to the query:

binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 297, 298

3c4jA Abc protein artp in complex with atp-gamma-s
31% identity, 84% coverage: 19:236/260 of query aligns to 17:233/242 of 3c4jA

query
sites
3c4jA

D

E

I
x
V

F

L

N

K

D

G

V

I

N

N

L

V

S

H

L

I

E

R

N

E

G

G

D

E

V

V

L

V

C

V

I

V

L

I

G

G

P

P

N
x
S

G
|
G

T
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

I

L

K

R

C

C

M

L

N

N

G

L

L

L

L

E

K

D

L

F

N

D

S

E

G

G

N

E

I

I

L

I

I

I

N

D

-

G

-

I

N

N

Q

L

S

K

I

A

H

K

S

D

L

T

N

N

K

L

N

N

D

K

L

V

A

R

K

E

I

E

I

V

G

G

Y

M

I

V

P

F

Q

Q

S

R

H

F

N

N

S

L

T

F

F

P

A

H

F

M

S

T

V

V

F

L

D

N

V

N

V

I

L

T

M

L

G

-

R

-

A

A

P

P

H

-

L

M

S

K

L

V

T

R

S

K

V

W

P

P

G

R

E

E

K

K

D

A

C

E

K

A

I

K

A

A

E

M

E

E

A

L

L

L

K

D

S

K

L

V

G

G

I

L

S

K

H

D

L

K

T

A

D

H

K

A

T

Y

Y

P

T

D

E

S

I

L

S

S

G

G

E

Q

R

A

Q

Q

M

R

V

V

L

A

I

I

A

A

R

R

V

A

L

L

A

A

Q

M

Q

E

P

P

Q

K

I

I

L

M

L

L

L

F

D

D

E

E

P

P

T

T

S

S

H

A

L

L

D

D

F

P

G

E

N

M

Q

V

I

G

R

E

T

V

L

L

D

S

V

V

I

M

N

K

K

Q

L

L

A

A

E

N

N

E

G

G

L

M

S

T

V

M

I

V

M

V

T

V

S

T

H

H

F

E

P

M

D

G

H

F

A

A

F

R

L

E

S

V

S

G

N

D

K

R

V

V

A

L

I

F

M

M

N

D

G

G

T

Y

I

I

M

I

E

E

M

E

G

G

T

K

P

P

E

E

S

D

V

L

I

F

T

D

E

R

E

P

N

Q

M

H

R

E

R

R

binding phosphothiophosphoric acid-adenylate ester: V18 (≠ I20), S38 (≠ N40), G39 (= G41), S40 (≠ T42), G41 (= G43), K42 (= K44), S43 (= S45), T44 (= T46)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 13

3c41J Abc protein artp in complex with amp-pnp/mg2+
31% identity, 84% coverage: 19:236/260 of query aligns to 17:233/242 of 3c41J

query
sites
3c41J

D

E

I
x
V

F

L

N

K

D

G

V

I

N

N

L

V

S

H

L

I

E

R

N

E

G

G

D

E

V

V

L

V

C

V

I

V

L

I

G

G

P

P

N
x
S

G
|
G

T

S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

I

L

K

R

C

C

M

L

N

N

G

L

L

L

L

E

K

D

L

F

N

D

S

E

G

G

N

E

I

I

L

I

I

I

N

D

-

G

-

I

N

N

Q

L

S

K

I

A

H

K

S

D

L

T

N

N

K

L

N

N

D

K

L

V

A

R

K

E

I

E

I

V

G

G

Y

M

I

V

P

F

Q

Q

S

R

H

F

N

N

S

L

T

F

F

P

A

H

F

M

S

T

V

V

F

L

D

N

V

N

V

I

L

T

M

L

G

-

R

-

A

A

P

P

H

-

L

M

S

K

L

V

T

R

S

K

V

W

P

P

G

R

E

E

K

K

D

A

C

E

K

A

I

K

A

A

E

M

E

E

A

L

L

L

K

D

S

K

L

V

G

G

I

L

S

K

H

D

L

K

T

A

D

H

K

A

T

Y

Y

P

T

D

E

S

I

L

S

S

G

G

E

Q

R

A

Q

Q

M

R

V

V

L

A

I

I

A

A

R

R

V

A

L

L

A

A

Q

M

Q

E

P

P

Q

K

I

I

L

M

L

L

L

F

D
|
D

E

E

P

P

T

T

S

S

H

A

L

L

D

D

F

P

G

E

N

M

Q

V

I

G

R

E

T

V

L

L

D

S

V

V

I

M

N

K

K

Q

L

L

A

A

E

N

N

E

G

G

L

M

S

T

V

M

I

V

M

V

T

V

S

T

H

H

F

E

P

M

D

G

H

F

A

A

F

R

L

E

S

V

S

G

N

D

K

R

V

V

A

L

I

F

M

M

N

D

G

G

T

Y

I

I

M

I

E

E

M

E

G

G

T

K

P

P

E

E

S

D

V

L

I

F

T

D

E

R

E

P

N

Q

M

H

R

E

R

R

binding phosphoaminophosphonic acid-adenylate ester: V18 (≠ I20), S38 (≠ N40), G39 (= G41), G41 (= G43), K42 (= K44), S43 (= S45), T44 (= T46)
binding magnesium ion: S43 (= S45), D163 (= D166)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 13

2olkA Abc protein artp in complex with adp-beta-s
31% identity, 84% coverage: 19:236/260 of query aligns to 17:233/242 of 2olkA

query
sites
2olkA

D

E

I
x
V

F

L

N

K

D

G

V

I

N

N

L

V

S

H

L

I

E

R

N

E

G

G

D

E

V

V

L

V

C

V

I

V

L

I

G

G

P

P

N
x
S

G
|
G

T
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

I

L

K

R

C

C

M

L

N

N

G

L

L

L

L

E

K

D

L

F

N

D

S

E

G

G

N

E

I

I

L

I

I

I

N

D

-

G

-

I

N

N

Q

L

S

K

I

A

H

K

S

D

L

T

N

N

K

L

N

N

D

K

L

V

A

R

K

E

I

E

I

V

G

G

Y

M

I

V

P

F

Q

Q

S

R

H

F

N

N

S

L

T

F

F

P

A

H

F

M

S

T

V

V

F

L

D

N

V

N

V

I

L

T

M

L

G

-

R

-

A

A

P

P

H

-

L

M

S

K

L

V

T

R

S

K

V

W

P

P

G

R

E

E

K

K

D

A

C

E

K

A

I

K

A

A

E

M

E

E

A

L

L

L

K

D

S

K

L

V

G

G

I

L

S

K

H

D

L

K

T

A

D

H

K

A

T

Y

Y

P

T

D

E

S

I

L

S

S

G

G

E

Q

R

A

Q

Q

M

R

V

V

L

A

I

I

A

A

R

R

V

A

L

L

A

A

Q

M

Q

E

P

P

Q

K

I

I

L

M

L

L

L

F

D

D

E

E

P

P

T

T

S

S

H

A

L

L

D

D

F

P

G

E

N

M

Q

V

I

G

R

E

T

V

L

L

D

S

V

V

I

M

N

K

K

Q

L

L

A

A

E

N

N

E

G

G

L

M

S

T

V

M

I

V

M

V

T

V

S

T

H

H

F

E

P

M

D

G

H

F

A

A

F

R

L

E

S

V

S

G

N

D

K

R

V

V

A

L

I

F

M

M

N

D

G

G

T

Y

I

I

M

I

E

E

M

E

G

G

T

K

P

P

E

E

S

D

V

L

I

F

T

D

E

R

E

P

N

Q

M

H

R

E

R

R

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: V18 (≠ I20), S38 (≠ N40), G39 (= G41), S40 (≠ T42), G41 (= G43), K42 (= K44), S43 (= S45), T44 (= T46)

Sites not aligning to the query:

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: 13

2oljA Abc protein artp in complex with adp/mg2+
31% identity, 84% coverage: 19:236/260 of query aligns to 17:233/242 of 2oljA

query
sites
2oljA

D

E

I
x
V

F

L

N

K

D

G

V

I

N

N

L

V

S

H

L

I

E

R

N

E

G

G

D

E

V

V

L

V

C

V

I

V

L

I

G

G

P

P

N
x
S

G
|
G

T
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

I

L

K

R

C

C

M

L

N

N

G

L

L

L

L

E

K

D

L

F

N

D

S

E

G

G

N

E

I

I

L

I

I

I

N

D

-

G

-

I

N

N

Q

L

S

K

I

A

H

K

S

D

L

T

N

N

K

L

N

N

D

K

L

V

A

R

K

E

I

E

I

V

G

G

Y

M

I

V

P

F

Q

Q

S

R

H

F

N

N

S

L

T

F

F

P

A

H

F

M

S

T

V

V

F

L

D

N

V

N

V

I

L

T

M

L

G

-

R

-

A

A

P

P

H

-

L

M

S

K

L

V

T

R

S

K

V

W

P

P

G

R

E

E

K

K

D

A

C

E

K

A

I

K

A

A

E

M

E

E

A

L

L

L

K

D

S

K

L

V

G

G

I

L

S

K

H

D

L

K

T

A

D

H

K

A

T

Y

Y

P

T

D

E

S

I

L

S

S

G

G

E

Q

R

A

Q

Q

M

R

V

V

L

A

I

I

A

A

R

R

V

A

L

L

A

A

Q

M

Q

E

P

P

Q

K

I

I

L

M

L

L

L

F

D

D

E

E

P

P

T

T

S

S

H

A

L

L

D

D

F

P

G

E

N

M

Q

V

I

G

R

E

T

V

L

L

D

S

V

V

I

M

N

K

K

Q

L

L

A

A

E

N

N

E

G

G

L

M

S

T

V

M

I

V

M

V

T

V

S

T

H

H

F

E

P

M

D

G

H

F

A

A

F

R

L

E

S

V

S

G

N

D

K

R

V

V

A

L

I

F

M

M

N

D

G

G

T

Y

I

I

M

I

E

E

M

E

G

G

T

K

P

P

E

E

S

D

V

L

I

F

T

D

E

R

E

P

N

Q

M

H

R

E

R

R

binding adenosine-5'-diphosphate: V18 (≠ I20), S38 (≠ N40), G39 (= G41), S40 (≠ T42), G41 (= G43), K42 (= K44), S43 (= S45), T44 (= T46)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 13

7ahdC Opua (e190q) occluded (see paper)
33% identity, 80% coverage: 23:230/260 of query aligns to 44:252/260 of 7ahdC

query
sites
7ahdC

D

D

V

T

N

N

L

F

S

E

L

I

E

N

N

E

G

G

D

E

V

I

L

F

C

V

I

I

L

M

G

G

P

L

N
x
S

G
|
G

T

S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

L

K

R

C

L

M

L

N

N

G

R

L

L

L

I

K

E

L

P

N

T

S

S

G

G

N

K

I

I

L

F

I

I

N

D

N

N

Q

Q

S

D

I

V

H

A

S

T

L

L

N

N

K

K

N

E

D

D

L

L

A

L

K

Q

I

V

I

R

G

R

Y

K

I

T

P

M

Q

S

S

M

H

V

N

F

S
x
Q

T

N

F

F

A

G

F

L

S

F

V

P

F

H

D

R

V

T

V

I

L

L

M

E

G

N

R

T

A

E

P

Y

H

G

L

L

S

E

L

V

T

Q

S

N

V

V

P

P

G

K

E

E

K

E

D

R

C

R

K

K

I

R

A

A

E

E

E

K

A

A

L

L

K

D

S

N

L

A

G

N

I

L

S

L

H

D

L

F

T

K

D

D

K

Q

T

Y

Y

P

T
x
K

E
x
Q

I

L

S
|
S

G

G

G
|
G

E
x
M

R

Q

Q

Q

M

R

V

V

L

G

I

L

A

A

R

R

V

A

L

L

A

A

Q

N

Q

D

P

P

Q

E

I

I

L

L

L

M

D

D

E
x
Q

P

A

T

F

S

S

H

A

L

L

D

D

F

P

G

L

N

I

Q

R

I

R

R

E

T

M

L

Q

D

D

V

E

I

L

N

L

K

E

L

L

-

Q

A

A

E

K

N

F

G

Q

L

K

S

T

V

I

I

I

M

F

T

V

S

S

H
|
H

F

D

P

L

D

N

H

E

A

A

F

L

L

R

S

I

S

G

N

D

K

R

V

I

A

A

I

I

M

M

N

K

G

D

G

G

T

K

I

I

M

M

E

Q

M

I

G

G

T

T

P

G

E

E

S

E

V

I

I

L

T

T

binding adenosine-5'-triphosphate: S61 (≠ N40), G62 (= G41), G64 (= G43), K65 (= K44), S66 (= S45), T67 (= T46), Q111 (≠ S90), K161 (≠ T140), Q162 (≠ E141), S164 (= S143), G166 (= G145), M167 (≠ E146), Q188 (≠ E167), H221 (= H199)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12, 39

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
30% identity, 87% coverage: 19:245/260 of query aligns to 15:240/240 of 4ymuJ

query
sites
4ymuJ

D

E

I
x
V

F

L

N

K

D

G

V

V

N

T

L

L

S

K

L

V

E

N

N

K

G

G

D

E

V

V

L

V

C

V

I

I

L

I

G

G

P

P

N
x
S

G
|
G

T
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

L

K

R

C

C

M

I

N

N

G

L

L

L

L

E

K

E

L

P

N

T

S

K

G

G

N

E

I

V

L

F

I

I

N

D

N

G

Q

V

S

K

I

I

H

N

S

N

-

G

-

K

L

V

N

N

K

I

N

N

D

K

L

V

A

R

K

Q

I

K

I

V

G

G

Y

M

I

V

P

F

Q

Q

S

H

H

F

N

N

S

L

T

F

F

P

A

H

F

L

S

T

V

A

F

I

D

E

V

N

V

I

L

T

M

L

G

-

R

-

A

A

P

P

H

-

L

V

S

K

L

V

T

K

S

K

V

M

P

N

G

K

E

K

K

E

D

A

C

E

K

E

I

L

A

A

E

V

E

D

A

L

L

L

K

A

S

K

L

V

G

G

I

L

S

L

H

D

L

K

T

K

D

D

K

Q

T

Y

Y

P

T

I

E

K

I

L

S

S

G

G

E

Q

R

K

Q

Q

M

R

V

L

L

A

I

I

A

A

R

R

V

A

L

L

A

A

Q

M

Q

Q

P

P

Q

E

I

V

L

M

L

L

L

F

D

D

E
|
E

P

P

T

T

S

S

H

A

L

L

D

D

F

P

G

E

N

M

Q

V

I

K

R

E

T

V

L

L

D

N

V

V

I

M

N

K

K

Q

L

L

A

A

E

N

N

E

G

G

L

M

S

T

V

M

I

V

M

V

T

V

S

T

H
|
H

F

E

P

M

D

G

H

F

A

A

F

R

L

E

S

V

S

G

N

D

K

R

V

V

A

I

I

F

M

M

N

D

G

D

G

G

T

V

I

I

M

V

E

E

M

E

G

G

T

T

P

P

E

E

S

E

V

I

I

F

T

Y

E

R

E

A

N

K

M

N

R

E

R

R

A

T

Y

R

N

E

I

F

D

L

V

S

K

K

I

I

M

L

binding adenosine-5'-triphosphate: V16 (≠ I20), S36 (≠ N40), G37 (= G41), S38 (≠ T42), G39 (= G43), K40 (= K44), S41 (= S45), T42 (= T46), E162 (= E167), H194 (= H199)
binding magnesium ion: S41 (= S45), E162 (= E167)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 11

5d3mA Folate ecf transporter: amppnp bound state (see paper)
29% identity, 92% coverage: 4:242/260 of query aligns to 5:244/280 of 5d3mA

query
sites
5d3mA

I

I

L

I

E

S

I

F

K

D

H

H

A

V

G

T

F
|
F

S

T

Y

Y

-

P

N

D

Q

S

K

P

E

R

D

P

I

A

F

L

N

S

D

D

V

L

N

S

L

F

S

A

L

I

E

E

N

R

G

G

D

S

V

W

L

T

C

A

I

L

L

I

G

G

P

H

N
|
N

G
|
G

T

S

G

G

K
|
K

S
|
S

T

T

L

V

I

S

K

K

C

L

M

I

N

N

G

G

L

L

-

A

-

P

-

D

K

D

L

L

N

D

S

K

G

S

N

S

I

I

L

T

I

V

N

D

N

G

Q

V

S

K

I

L

H

G

S

A

L

D

N

T

K

V

N

W

D

E

L

V

A

R

K

E

I

K

I

V

G

G

Y

I

I

V

P

F

Q

Q

S

N

H

P

N

D

S

N

T

Q

F

F

-

V

A

G

F

A

S

T

V

V

F

S

D

D

V

D

V

V

L

A

M

F

G

G

R

-

A

-

P

-

H

-

L

L

S

E

L

N

T

R

S

A

V

V

P

P

G

R

E

P

K

E

D

M

C

L

K

K

I

I

A

V

E

A

E

Q

A

A

L

V

K

A

S

D

L

V

G

G

I

M

S

A

H

D

L

Y

T

A

D

D

K

S

T

E

Y

P

T

S

E

N

I

L

S

S

G

G

E

Q

R

K

Q

Q

M

R

V

V

L

A

I

I

A

A

R

G

V

I

L

L

A

A

Q

V

Q

K

P

P

Q

Q

I

V

L

I

L

L

D

D

E

E

P

S

T

T

S

S

H

M

L

L

D

D

F

P

G

E

N

G

Q

K

I

E

R

Q

T

I

L

L

D

D

V

L

I

V

N

R

K

K

L

I

A

K

E

E

-

D

N

N

G

N

L

L

S

T

V

V

I

I

M

S

T

I

S

T

H

H

F

D

P

L

D

E

H

E

A

A

F

-

L

A

S

G

S

A

N

D

K

Q

V

V

A

L

I

V

M

L

N

D

G

D

G

G

T

Q

I

L

M

L

E

D

M

Q

G

G

T

K

P

P

E

E

S

E

V

I

I

F

T

P

E

K

E

V

N

E

M

M

R

L

R

K

A

R

Y

I

N

G

I

L

D

D

V

I

binding phosphoaminophosphonic acid-adenylate ester: F13 (= F12), N42 (= N40), G43 (= G41), K46 (= K44), S47 (= S45)

Q5M243 Energy-coupling factor transporter ATP-binding protein EcfA1; ECF transporter A component EcfA1; EC 7.-.-.- from Streptococcus thermophilus (strain ATCC BAA-250 / LMG 18311) (see paper)
31% identity, 87% coverage: 4:230/260 of query aligns to 1:226/276 of Q5M243

query
sites
Q5M243

I

M

L

I

E

E

I

I

K

K

H

N

A

L

G

K

F

F

S

K

Y

Y

N

N

Q

Q

K

D

E

Q

D

T

I

S

F

Y

-

T

-

L

N

N

D

D

V

V

N

S

L

F

S

H

L

V

E

K

N

H

G

G

D

E

V

W

L

L

C

S

I

I

L

V

G

G

P

H

N

N

G

G

T

S

G

G

K

K

S

S

T

T

L

T

I

A

K

R

C

L

M

I

N

G

G

G

L

L

K

V

L

A

N

D

S

S

G

G

N

Q

I

I

L

I

I

V

N

D

N

G

Q

Q

S

E

I

L

H

T

S

E

L

E

N

T

K

V

N

W

D

D

L

I

A

R

K

D

I

K

I

I

G

G

Y

M

I

V

P

F

Q

Q

S

N

H

P

N

D

S

N

T
x
Q

F

F

-

V

A

G

F

A

S

T

V

V

F

E

D

D

V

D

V

V

L

A

M

F

G

G

R

-

A

-

P

-

H

-

L

L

S

E

L

N

T

K

S

G

V

L

P

P

G

Y

E

K

K

E

D

M

C

V

K

S

I

R

A

V

E

Q

E

E

A

A

L

L

K

S

S

F

L

V

G

G

I

M

S

M

H

D

L

F

T

K

D

D

K

R

T

E

Y

P

T

A

E

R

I

L

S

S

G

G

E

Q

R

K

Q

Q

M

R

V

V

L

A

I

I

A

A

R

G

V

I

L

I

A

A

Q

M

Q

R

P

P

Q

S

I

I

L

L

L

I

L

L

D

D

E
|
E

P

A

T

T

S

S

H

M

L

L

D

D

F

P

G

E

N

G

Q

R

I

Q

R

E

T

L

L

I

D

Q

V

Y

I

I

N

E

K

D

L

I

A

R

E

Q

N

Q

-

Y

G

G

L

M

S

T

V

V

I

L

M

S

T

I

S

T

H

H

F

D

P

L

D

D

H

E

A

V

F

A

L

M

S

-

S

S

N

N

K

R

V

V

A

L

I

V

M

L

N

K

G

Q

G

G

T

K

I

V

M

E

E

S

M

I

G

S

T

S

P

P

E

R

S

E

V

L

I

F

T

S

Q90 (≠ T91) mutation to A: No effect on ATPase, 10-fold decrease in riboflavin uptake; when associated with A-97 in EcfA2.
E163 (= E167) mutation to Q: 10-fold decrease in ATPase and riboflavin uptake; when associated with Q-171 in EcfA2.

6b8bA E. Coli lptb in complex with adp and a novobiocin derivative (see paper)
32% identity, 84% coverage: 20:238/260 of query aligns to 17:233/233 of 6b8bA

query
sites
6b8bA

I
x
V

F

V

N

E

D

D

V

V

N

S

L

L

S

T

L

V

E

N

N

S

G

G

D

E

V

I

L

V

C

G

I

L

L

L

G

G

P

P

N

N

G
|
G

T

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

I

F

K

Y

C

M

M

V

N

V

G

G

L

I

L

V

K

P

L

R

N

D

S

A

G

G

N

N

I

I

L

I

I

I

N

D

Q

D

S

D

I

I

H

S

S

L

L

L

N

P

K

L

N

H

D

A

L

R

A

A

K

R

I

R

-

G

I

I

G

G

Y

Y

I

L

P

P

Q

Q

S

E

H

A

N

S

S

I

T
x
F

F
x
R

A
x
R

F

L

S

S

V

V

F

Y

D

D

-

N

-

L

-

M

V

A

V

V

L

L

M

Q

G

I

R

R

A

-

P

-

H

-

L

-

S

-

L

-

T

D

S

D

V

L

P

S

G

A

E

E

K

Q

D

R

C

E

K

D

I

R

A

A

E

N

E

E

A

L

L

M

K

E

S

E

L

F

G

H

I

I

S

E

H

H

L

L

T

R

D

D

K

S

T

M

Y

G

T

Q

E

S

I

L

S

S

G

G

E

E

R

R

Q

R

M

R

V

V

L

E

I

I

A

A

R

R

V

A

L

L

A

A

Q

A

Q

N

P

P

Q

K

I

F

L

I

L

L

D

D

E

E

P

P

T

F

S

A

H

G

L

V

D

D

F

P

G

I

N

S

Q

V

I

I

R

D

T

I

L

K

D

R

V

I

I

I

N

E

K

H

L

L

A

R

E

D

N

S

G

G

L

L

S

G

V

V

I

L

M

I

T

T

S

D

H

H

F

N

P

V

D

R

H

E

A

T

F

L

L

A

S

V

S

C

N

E

K

R

V

A

A

Y

I

I

M

V

N

S

G

Q

G

G

T

H

I

L

M

I

E

A

M

H

G

G

T

T

P

P

E

T

S

E

V

I

I

L

T

Q

E

D

E

E

N

H

M

V

R

K

R

R

A

V

Y

Y

binding adenosine-5'-diphosphate: V17 (≠ I20), G38 (= G41), G40 (= G43), K41 (= K44), T42 (≠ S45), T43 (= T46)
binding (3s,5s,7s)-N-{7-[(3-O-carbamoyl-6-deoxy-5-methyl-4-O-methyl-beta-D-gulopyranosyl)oxy]-4-hydroxy-8-methyl-2-oxo-2H-1-benzopyran-3-yl}tricyclo[3.3.1.1~3,7~]decane-1-carboxamide: F89 (≠ T91), R90 (≠ F92), R91 (≠ A93)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 15

6b89A E. Coli lptb in complex with adp and novobiocin (see paper)
32% identity, 84% coverage: 20:238/260 of query aligns to 17:233/234 of 6b89A

query
sites
6b89A

I
x
V

F

V

N

E

D

D

V

V

N

S

L

L

S

T

L

V

E

N

N

S

G

G

D

E

V

I

L

V

C

G

I

L

L

L

G

G

P

P

N
|
N

G
|
G

T

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

I

F

K

Y

C

M

M

V

N

V

G

G

L

I

L

V

K

P

L

R

N

D

S

A

G

G

N

N

I

I

L

I

I

I

N

D

Q

D

S

D

I

I

H

S

S

L

L

L

N

P

K
x
L

N
x
H

D

A

L

R

A

A

K

R

I

R

-

G

I

I

G

G

Y

Y

I

L

P
|
P

Q
|
Q

S

E

H
x
A

N
x
S

S

I

T
x
F

F
x
R

A
x
R

F
x
L

S

S

V

V

F

Y

D

D

-

N

-

L

-

M

V

A

V
|
V

L

L

M

Q

G

I

R

R

A

-

P

-

H

-

L

-

S

-

L

-

T

D

S

D

V

L

P

S

G

A

E

E

K

Q

D

R

C

E

K

D

I

R

A

A

E

N

E

E

A

L

L

M

K

E

S

E

L

F

G

H

I

I

S

E

H

H

L

L

T

R

D

D

K

S

T

M

Y

G

T
x
Q

E

S

I

L

S

S

G

G

E

E

R

R

Q

R

M

R

V

V

L

E

I

I

A

A

R
|
R

V

A

L

L

A

A

Q

A

Q

N

P

P

Q

K

I

F

L

I

L

L

D

D

E

E

P

P

T

F

S

A

H

G

L

V

D

D

F

P

G

I

N

S

Q

V

I

I

R

D

T

I

L

K

D

R

V

I

I

I

N

E

K

H

L

L

A

R

E

D

N

S

G

G

L

L

S

G

V

V

I

L

M

I

T

T

S

D

H

H

F

N

P

V

D

R

H

E

A

T

F

L

L

A

S

V

S

C

N

E

K

R

V

A

A

Y

I

I

M

V

N

S

G

Q

G

G

T

H

I

L

M

I

E

A

M

H

G

G

T

T

P

P

E

T

S

E

V

I

I

L

T

Q

E

D

E

E

N

H

M

V

R

K

R

R

A

V

Y

Y

binding adenosine-5'-diphosphate: V17 (≠ I20), N37 (= N40), G38 (= G41), G40 (= G43), K41 (= K44), T42 (≠ S45), T43 (= T46)
binding magnesium ion: T42 (≠ S45), Q84 (= Q86)
binding novobiocin: L71 (≠ K74), H72 (≠ N75), P83 (= P85), A86 (≠ H88), S87 (≠ N89), F89 (≠ T91), R90 (≠ F92), R91 (≠ A93), L92 (≠ F94), V101 (= V100), Q135 (≠ T140), R149 (= R154)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 15

4p31A Crystal structure of a selenomethionine derivative of e. Coli lptb in complex with adp-magensium (see paper)
32% identity, 84% coverage: 20:238/260 of query aligns to 17:233/234 of 4p31A

query
sites
4p31A

I
x
V

F

V

N

E

D

D

V

V

N

S

L

L

S

T

L

V

E

N

N

S

G

G

D

E

V

I

L

V

C

G

I

L

L

L

G

G

P

P

N

N

G
|
G

T

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

I

F

K

Y

C

M

M

V

N

V

G

G

L

I

L

V

K

P

L

R

N

D

S

A

G

G

N

N

I

I

L

I

I

I

N

D

Q

D

S

D

I

I

H

S

S

L

L

L

N

P

K

L

N

H

D

A

L

R

A

A

K

R

I

R

-

G

I

I

G

G

Y

Y

I

L

P

P

Q
|
Q

S

E

H

A

N

S

S

I

T

F

F

R

A

R

F

L

S

S

V

V

F

Y

D

D

-

N

-

L

-

M

V

A

V

V

L

L

M

Q

G

I

R

R

A

-

P

-

H

-

L

-

S

-

L

-

T

D

S

D

V

L

P

S

G

A

E

E

K

Q

D

R

C

E

K

D

I

R

A

A

E

N

E

E

A

L

L

M

K

E

S

E

L

F

G

H

I

I

S

E

H

H

L

L

T

R

D

D

K

S

T

M

Y

G

T

Q

E

S

I

L

S

S

G

G

E

E

R

R

Q

R

M

R

V

V

L

E

I

I

A

A

R

R

V

A

L

L

A

A

Q

A

Q

N

P

P

Q

K

I

F

L

I

L

L

D

D

E

E

P

P

T

F

S

A

H

G

L

V

D

D

F

P

G

I

N

S

Q

V

I

I

R

D

T

I

L

K

D

R

V

I

I

I

N

E

K

H

L

L

A

R

E

D

N

S

G

G

L

L

S

G

V

V

I

L

M

I

T

T

S

D

H

H

F

N

P

V

D

R

H

E

A

T

F

L

L

A

S

V

S

C

N

E

K

R

V

A

A

Y

I

I

M

V

N

S

G

Q

G

G

T

H

I

L

M

I

E

A

M

H

G

G

T

T

P

P

E

T

S

E

V

I

I

L

T

Q

E

D

E

E

N

H

M

V

R

K

R

R

A

V

Y

Y

binding adenosine-5'-diphosphate: V17 (≠ I20), G38 (= G41), G40 (= G43), K41 (= K44), T42 (≠ S45), T43 (= T46)
binding magnesium ion: T42 (≠ S45), Q84 (= Q86)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 15

6mhzA Vanadate trapped cryo-em structure of e.Coli lptb2fg transporter (see paper)
32% identity, 84% coverage: 20:238/260 of query aligns to 17:233/235 of 6mhzA

query
sites
6mhzA

I

V

F

V

N

E

D

D

V

V

N

S

L

L

S

T

L

V

E

N

N

S

G

G

D

E

V

I

L

V

C

G

I

L

L

L

G

G

P

P

N
|
N

G
|
G

T

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

I

F

K

Y

C

M

M

V

N

V

G

G

L

I

L

V

K

P

L

R

N

D

S

A

G

G

N

N

I

I

L

I

I

I

N

D

Q

D

S

D

I

I

H

S

S

L

L

L

N

P

K

L

N

H

D

A

L

R

A

A

K

R

I

R

-

G

I

I

G

G

Y

Y

I

L

P

P

Q
|
Q

S

E

H

A

N

S

S

I

T

F

F

R

A

R

F

L

S

S

V

V

F

Y

D

D

-

N

-

L

-

M

V

A

V

V

L

L

M

Q

G

I

R

R

A

-

P

-

H

-

L

-

S

-

L

-

T

D

S

D

V

L

P

S

G

A

E

E

K

Q

D

R

C

E

K

D

I

R

A

A

E

N

E

E

A

L

L

M

K

E

S

E

L

F

G

H

I

I

S

E

H

H

L

L

T

R

D

D

K

S

T

M

Y

G

T

Q

E
x
S

I

L

S
|
S

G
|
G

E

E

R

R

Q

R

M

R

V

V

L

E

I

I

A

A

R

R

V

A

L

L

A

A

Q

A

Q

N

P

P

Q

K

I

F

L

I

L

L

D

D

E
|
E

P

P

T

F

S

A

H
x
G

L

V

D

D

F

P

G

I

N

S

Q

V

I

I

R

D

T

I

L

K

D

R

V

I

I

I

N

E

K

H

L

L

A

R

E

D

N

S

G

G

L

L

S

G

V

V

I

L

M

I

T

T

S

D

H
|
H

F

N

P

V

D

R

H

E

A

T

F

L

L

A

S

V

S

C

N

E

K

R

V

A

A

Y

I

I

M

V

N

S

G

Q

G

G

T

H

I

L

M

I

E

A

M

H

G

G

T

T

P

P

E

T

S

E

V

I

I

L

T

Q

E

D

E

E

N

H

M

V

R

K

R

R

A

V

Y

Y

binding adp orthovanadate: N37 (= N40), G38 (= G41), G40 (= G43), K41 (= K44), T42 (≠ S45), T43 (= T46), Q84 (= Q86), S136 (≠ E141), S138 (= S143), G139 (= G144), G140 (= G145), E162 (= E167), G166 (≠ H171), H194 (= H199)

Sites not aligning to the query:

binding adp orthovanadate: 12

6s8nB Cryo-em structure of lptb2fgc in complex with lipopolysaccharide (see paper)
32% identity, 84% coverage: 20:238/260 of query aligns to 17:233/238 of 6s8nB

query
sites
6s8nB

I

V

F

V

N

E

D

D

V

V

N

S

L

L

S

T

L

V

E

N

N

S

G

G

D

E

V

I

L

V

C

G

I

L

L

L

G

G

P

P

N

N

G

G

T

A

G

G

K

K

S

T

T

T

L

T

I

F

K

Y

C

M

M

V

N

V

G

G

L

I

L

V

K

P

L

R

N

D

S

A

G

G

N

N

I

I

L

I

I

I

N

D

Q

D

S

D

I

I

H

S

S

L

L

L

N

P

K

L

N

H

D

A

L

R

A

A

K

R

I

R

-

G

I

I

G

G

Y

Y

I

L

P

P

Q

Q

S

E

H

A

N

S

S

I

T

F

F
x
R

A

R

F

L

S

S

V

V

F

Y

D

D

-

N

-

L

-

M

V

A

V

V

L

L

M

Q

G

I

R

R

A

-

P

-

H

-

L

-

S

-

L

-

T

D

S

D

V

L

P

S

G

A

E

E

K

Q

D

R

C

E

K

D

I

R

A

A

E

N

E

E

A

L

L

M

K

E

S

E

L

F

G

H

I

I

S

E

H

H

L

L

T

R

D

D

K

S

T
x
M

Y
x
G

T
x
Q

E

S

I

L

S

S

G

G

E

E

R

R

Q

R

M

R

V

V

L

E

I

I

A

A

R

R

V

A

L

L

A

A

Q

A

Q

N

P

P

Q

K

I

F

L

I

L

L

D

D

E

E

P

P

T

F

S

A

H

G

L

V

D

D

F

P

G

I

N

S

Q

V

I

I

R

D

T

I

L

K

D

R

V

I

I

I

N

E

K

H

L

L

A

R

E

D

N

S

G

G

L

L

S

G

V

V

I

L

M

I

T

T

S

D

H

H

F

N

P

V

D

R

H

E

A

T

F

L

L

A

S

V

S

C

N

E

K

R

V

A

A

Y

I

I

M

V

N

S

G

Q

G

G

T

H

I

L

M

I

E

A

M

H

G

G

T

T

P

P

E

T

S

E

V

I

I

L

T

Q

E

D

E

E

N

H

M

V

R

K

R

R

A

V

Y

Y

binding 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione: R90 (≠ F92), M133 (≠ T138), G134 (≠ Y139), Q135 (≠ T140)

6s8gA Cryo-em structure of lptb2fgc in complex with amp-pnp (see paper)
32% identity, 84% coverage: 20:238/260 of query aligns to 17:233/238 of 6s8gA

query
sites
6s8gA

I

V

F

V

N

E

D

D

V

V

N

S

L

L

S

T

L

V

E

N

N

S

G

G

D

E

V

I

L

V

C

G

I

L

L

L

G

G

P

P

N
|
N

G

G

T

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

I

F

K

Y

C

M

M

V

N

V

G

G

L

I

L

V

K

P

L

R

N

D

S

A

G

G

N

N

I

I

L

I

I

I

N

D

Q

D

S

D

I

I

H

S

S

L

L

L

N

P

K

L

N

H

D

A

L

R

A

A

K

R

I

R

-

G

I

I

G

G

Y

Y

I

L

P

P

Q
|
Q

S

E

H

A

N

S

S

I

T

F

F

R

A

R

F

L

S

S

V

V

F

Y

D

D

-

N

-

L

-

M

V

A

V

V

L

L

M

Q

G

I

R

R

A

-

P

-

H

-

L

-

S

-

L

-

T

D

S

D

V

L

P

S

G

A

E

E

K

Q

D

R

C

E

K

D

I

R

A

A

E

N

E

E

A

L

L

M

K

E

S

E

L

F

G

H

I

I

S

E

H

H

L

L

T

R

D

D

K

S

T

M

Y

G

T

Q

E
x
S

I

L

S
|
S

G

G

E
|
E

R

R

Q

R

M

R

V

V

L

E

I

I

A

A

R

R

V

A

L

L

A

A

Q

A

Q

N

P

P

Q

K

I

F