SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_048081501.1 NCBI__GCF_000746075.1:WP_048081501.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
32% identity, 93% coverage: 2:236/252 of query aligns to 11:249/265 of P07821

query
sites
P07821

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K50 (= K41) mutation to Q: Lack of activity.
D172 (= D160) mutation to E: Lack of activity.
E173 (= E161) mutation to A: Lack of activity.

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
33% identity, 88% coverage: 2:223/252 of query aligns to 2:223/241 of 4u00A

query
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binding adenosine-5'-diphosphate: F12 (≠ Y12), V17 (= V17), S37 (≠ N37), G38 (= G38), S39 (= S39), G40 (= G40), K41 (= K41), S42 (= S42), T43 (= T43)

3c4jA Abc protein artp in complex with atp-gamma-s
33% identity, 87% coverage: 2:221/252 of query aligns to 3:223/242 of 3c4jA

query
sites
3c4jA

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binding phosphothiophosphoric acid-adenylate ester: F13 (≠ Y12), V18 (= V17), S38 (≠ N37), G39 (= G38), S40 (= S39), G41 (= G40), K42 (= K41), S43 (= S42), T44 (= T43)

3c41J Abc protein artp in complex with amp-pnp/mg2+
33% identity, 87% coverage: 2:221/252 of query aligns to 3:223/242 of 3c41J

query
sites
3c41J

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binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ Y12), V18 (= V17), S38 (≠ N37), G39 (= G38), G41 (= G40), K42 (= K41), S43 (= S42), T44 (= T43)
binding magnesium ion: S43 (= S42), D163 (= D160)

2olkA Abc protein artp in complex with adp-beta-s
33% identity, 87% coverage: 2:221/252 of query aligns to 3:223/242 of 2olkA

query
sites
2olkA

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binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (≠ Y12), V18 (= V17), S38 (≠ N37), G39 (= G38), S40 (= S39), G41 (= G40), K42 (= K41), S43 (= S42), T44 (= T43)

2oljA Abc protein artp in complex with adp/mg2+
33% identity, 87% coverage: 2:221/252 of query aligns to 3:223/242 of 2oljA

query
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2oljA

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D

I

T

E

N

I

L

A

N

R

K

-

V

-

R

-

E

K

E

I

V

G

G

Y

M

V

V

P

F

Q

Q

S

R

S

F

E

N

T

L

-

F

G

P

F

H

V

M

T

T

V

V

F

L

D

N

A

N

V

I

L

T

L

L

G

A

R

P

K

M

P

K

Y

V

I

R

K

K

W

W

D

P

I

R

S

E

K

K

K

A

D

E

I

A

E

K

L

A

T

M

E

E

N

L

I

L

L

D

K

K

V

V

M

-

N

G

L

L

E

K

E

D

Y

K

A

A

L

H

R

A

Y

Y

I

P

N

D

E

S

L

L

S

S

G

G

E

Q

L

A

Q

Q

K

R

V

V

V

A

I

I

A

A

R

R

A

A

L

L

V

A

Q

M

E

E

P

P

Q

K

I

I

L

M

L

L

L

F

D

D

E

E

P

P

T

T

S

S

D

A

L

L

D

D

L

P

K

E

N

M

Q

V

L

G

E

E

V

V

M

L

K

S

I

V

I

M

R

K

E

Q

V

L

S

A

D

N

T

-

H

E

N

G

I

M

A

T

S

M

I

V

V

V

M

T

H

H

D

E

I

M

N

G

L

F

A

A

L

R

R

E

Y

V

S

G

D

D

K

R

F

V

I

L

I

F

L

M

K

D

E

G

G

G

Q

Y

V

I

F

I

T

E

G

G

G

K

K

P

E

E

binding adenosine-5'-diphosphate: F13 (≠ Y12), V18 (= V17), S38 (≠ N37), G39 (= G38), S40 (= S39), G41 (= G40), K42 (= K41), S43 (= S42), T44 (= T43)

8g4cB Bceabs atpgs high res tm (see paper)
32% identity, 89% coverage: 1:224/252 of query aligns to 2:234/248 of 8g4cB

query
sites
8g4cB

M

V

V

I

L

L

S

E

V

A

D

N

K

K

I

I

E

R

F

K

S

S

Y
|
Y

G

G

N

N

-

K

-

L

-

N

-

K

A

Q

T

E

V

V

L

L

K

K

D

G

V

I

N

D

F

I

K

H

V

I

K

E

K

K

G

G

D

E

F

F

V

V

S

S

I

I

L

M

G

G

V

A

N
x
S

G

G

S
|
S

G

G

K

K

S
x
T

T

T

L

L

M

L

K

N

C

V

I

L

N

S

R

S

I

I

L

D

K

Q

F

V

K

S

E

H

G

G

M

T

I

I

F

H

V

I

E

N

D

G

R

N

D

D

L

M

K

T

K

A

M

M

K

K

D

E

I

K

E

Q

I

L

A

A

-

E

-

F

-

R

-

K

R

Q

K

H

I

L

G

G

Y

F

V

I

P

F

Q

Q

S

D

S

Y

E

N

T

L

-

L

G

D

F

T

V

L

T

T

V

V

F

K

D

E

A

N

V

I

L

L

G

-

R

-

K

-

P

P

Y

L

I

S

K

I

W

T

D

K

I

L

S

S

K

K

D

E

I

A

E

N

L

R

T

K

-

F

E

E

N

E

I

V

L

A

K

K

V

E

M

L

N

G

L

I

E

Y

E

E

Y

L

A

R

L

D

R

K

Y

Y

I

P

N

N

E

E

L

I

S

S

G

G

E

Q

L

K

Q

Q

K

R

V

T

V

S

I

A

A

G

R

R

A

A

L

F

V

I

Q

H

E

D

P

P

Q

S

I

I

L

I

L

F

L

A

D
|
D

E

E

P

P

T

T

S

G

D

A

L

L

D

D

L

S

K

K

N

S

Q

A

L

S

E

D

V

L

M

L

K

N

I

K

I

L

R

S

E

Q

V

L

S

N

D

Q

T

K

H

R

N

N

I

A

A

T

S

I

I

I

V

M

V

V

M

T

H

H

D

D

I

P

N

-

L

V

A

A

L

A

R

S

Y

Y

S

C

D

G

K

R

F

V

I

I

I

F

L

I

K

K

E

D

G

G

Q

Q

V

M

F

Y

T

T

T

Q

-

L

-

N

-

K

G

G

K

Q

E

D

V

R

I

Q

T

T

binding phosphothiophosphoric acid-adenylate ester: Y13 (= Y12), S42 (≠ N37), S44 (= S39), T47 (≠ S42), D168 (= D160)

7tchB Bceab e169q variant atp-bound conformation (see paper)
32% identity, 89% coverage: 1:224/252 of query aligns to 1:233/245 of 7tchB

query
sites
7tchB

M

V

V

I

L

L

S

E

V

A

D

N

K

K

I

I

E

R

F

K

S

S

Y
|
Y

G

G

N

N

-

K

-

L

-

N

-

K

A
x
Q

T

E

V
|
V

L

L

K

K

D

G

V

I

N

D

F

I

K

H

V

I

K

E

K

K

G

G

D

E

F

F

V

V

S

S

I

I

L

M

G

G

V

A

N
x
S

G
|
G

S
|
S

G

G

K
|
K

S
x
T

T

T

L

L

M

L

K

N

C

V

I

L

N

S

R

S

I

I

L

D

K

Q

F

V

K

S

E

H

G

G

M

T

I

I

F

H

V

I

E

N

D

G

R

N

D

D

L

M

K

T

K

A

M

M

K

K

D

E

I

K

E

Q

I

L

A

A

-

E

-

F

-

R

-

K

R

Q

K

H

I

L

G

G

Y

F

V

I

P

F

Q

Q

S

D

S

Y

E

N

T

L

-

L

G

D

F

T

V

L

T

T

V

V

F

K

D

E

A

N

V

I

L

L

G

-

R

-

K

-

P

P

Y

L

I

S

K

I

W

T

D

K

I

L

S

S

K

K

D

E

I

A

E

N

L

R

T

K

-

F

E

E

N

E

I

V

L

A

K

K

V

E

M

L

N

G

L

I

E

Y

E

E

Y

L

A

R

L

D

R

K

Y

Y

I

P

N

N

E
|
E

L

I

S

S

G
|
G

E

Q

L

K

Q

Q

K

R

V

T

V

S

I

A

A

G

R

R

A

A

L

F

V

I

Q

H

E

D

P

P

Q

S

I

I

L

I

L

F

L

A

D

D

E

Q

P

P

T

T

S

G

D

A

L

L

D

D

L

S

K

K

N

S

Q

A

L

S

E

D

V

L

M

L

K

N

I

K

I

L

R

S

E

Q

V

L

S

N

D

Q

T

K

H

R

N

N

I

A

A

T

S

I

I

I

V

M

V

V

M

T

H

H

D

D

I

-

N

P

L

V

A

A

L

A

R

S

Y

Y

S

C

D

G

K

R

F

V

I

I

I

F

L

I

K

K

E

D

G

G

Q

Q

V

M

F

Y

T

T

T

Q

-

L

-

N

-

K

G

G

K

Q

E

D

V

R

I

Q

T

T

binding adenosine-5'-triphosphate: Y12 (= Y12), Q19 (≠ A15), V21 (= V17), S41 (≠ N37), G42 (= G38), S43 (= S39), K45 (= K41), T46 (≠ S42), E142 (= E135), G145 (= G138), G146 (= G139)

1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
36% identity, 79% coverage: 17:214/252 of query aligns to 20:223/232 of 1f3oA

query
sites
1f3oA

V

A

L

L

K

K

D

N

V

V

N

N

F

L

K

N

V

I

K

K

K

E

G

G

D

E

F

F

V

V

S

S

I

I

L

M

G

G

V

P

N

S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

L

M

M

L

K

N

C

I

I

I

N

G

R

C

I

L

L

D

K

K

F

P

K

T

E

E

G

G

M

E

I

V

F

Y

V

I

E

D

D

N

R

I

D

K

L

T

K

N

K

D

M

L

K

D

D

D

I

D

E

E

I

L

A

T

R

K

-

I

-

R

-

D

K

K

I

I

G

G

Y

F

V

V

-

F

P

Q

Q

Q

S

F

S

N

E

L

T

I

G

P

F

L

V

L

T

T

V

A

F

L

D

E

A

N

V

V

L

E

L

L

G

-

R

-

K

-

P

P

Y

L

I

I

-

F

K

K

W

Y

D

R

I

G

S

A

K

M

K

S

D

G

I

E

E

E

L

R

T

R

E

K

N

R

I

A

L

L

K

E

V

C

M

L

N

K

L

M

E

A

E

E

Y

L

A

E

L

E

R

R

Y

F

I

A

N

N

-

H

-

K

-

P

-

N

E

Q

L

L

S

S

G

G

E

Q

L

Q

Q

Q

K

R

V

V

V

A

I

I

A

A

R

R

A

A

L

L

V

A

Q

N

E

N

P

P

Q

P

I

I

L

I

L

L

L

A

D

D

E

E

P

P

T

T

S

G

D

A

L

L

D

D

L

S

K

K

N

T

Q

G

L

E

E

K

V

I

M

M

K

Q

I

L

R

K

E

K

V

L

S

N

D

E

T

E

H

D

N

G

I

K

A

T

S

V

I

V

V

V

M

T

H

H

D

D

I

I

N

N

L

V

A

A

L

-

R

R

Y

F

S

G

D

E

K

R

F

I

I

I

I

Y

L

L

K

K

E

D

G

G

Q

E

V

V

binding adenosine-5'-diphosphate: G41 (= G38), G43 (= G40), K44 (= K41), S45 (= S42), T46 (= T43)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 11

1l2tA Dimeric structure of mj0796, a bacterial abc transporter cassette (see paper)
35% identity, 79% coverage: 17:214/252 of query aligns to 20:223/230 of 1l2tA

query
sites
1l2tA

V

A

L

L

K

K

D

N

V

V

N

N

F

L

K

N

V

I

K

K

K

E

G

G

D

E

F

F

V

V

S

S

I

I

L

M

G

G

V

P

N
x
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

M

M

L

K

N

C

I

I

I

N

G

R

C

I

L

L

D

K

K

F

P

K

T

E

E

G

G

M

E

I

V

F

Y

V

I

E

D

D

N

R

I

D

K

L

T

K

N

K

D

M

L

K

D

D

D

I

D

E

E

I

L

A

T

R

K

-

I

-

R

-

D

K

K

I

I

G

G

Y

F

V

V

-

F

P

Q

Q

Q

S

F

S

N

E

L

T

I

G

P

F

L

V

L

T

T

V

A

F

L

D

E

A

N

V

V

L

E

L

L

G

-

R

-

K

-

P

P

Y

L

I

I

-

F

K

K

W

Y

D

R

I

G

S

A

K

M

K

S

D

G

I

E

E

E

L

R

T

R

E

K

N

R

I

A

L

L

K

E

V

C

M

L

N

K

L

M

E

A

E

E

Y

L

A

E

L

E

R

R

Y
x
F

I

A

N

N

-

H

-

K

-

P

-

N

E
x
Q

L

L

S
|
S

G

G

G
|
G

E
x
Q

L

Q

Q

Q

K

R

V

V

V

A

I

I

A

A

R

R

A

A

L

L

V

A

Q

N

E

N

P

P

Q

P

I

I

L

I

L

L

L

A

D

D

E

Q

P

P

T

T

S

G

D

A

L

L

D

D

L

S

K

K

N

T

Q

G

L

E

E

K

V

I

M

M

K

Q

I

L

R

K

E

K

V

L

S

N

D

E

T

E

H

D

N

G

I

K

A

T

S

V

I

V

V

V

M

T

H
|
H

D

D

I

I

N

N

L

V

A

A

L

-

R

R

Y

F

S

G

D

E

K

R

F

I

I

I

I

Y

L

L

K

K

E

D

G

G

Q

E

V

V

binding adenosine-5'-triphosphate: S40 (≠ N37), G41 (= G38), S42 (= S39), G43 (= G40), K44 (= K41), S45 (= S42), T46 (= T43), F138 (≠ Y132), Q145 (≠ E135), S147 (= S137), G149 (= G139), Q150 (≠ E140), H204 (= H194)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 11

1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
32% identity, 82% coverage: 12:218/252 of query aligns to 16:211/353 of 1vciA

query
sites
1vciA

Y
x
F

G

G

N

N

A
x
F

T

T

V

A

L

V

K

N

D

K

V

L

N

N

F

L

K

T

V

I

K

K

K

D

G

G

D

E

F

F

V

L

S

V

I

L

L

L

G

G

V

P

N
x
S

G
|
G

S

C

G
|
G

K
|
K

S
x
T

T
|
T

L

T

M

L

K

R

C

M

I

I

N

A

R

G

I

L

L

E

K

E

F

P

K

T

E

E

G

G

M

R

I

I

F

Y

V

F

E

G

D

D

R

R

D

D

L

V

K

T

K

Y

M

L

K

P

D

P

I

K

E

D

I

-

A

-

R

R

K

N

I

I

G

S

Y

M

V

V

P

F

Q

Q

S

H

S

-

E

-

T

-

G

-

F

-

V

M

T

T

V

V

F

Y

D

E

A

N

V

I

L

A

L

F

G

P

R

L

K

K

P

K

Y

F

I

P

K

K

W

D

D

E

I

I

S

D

K

K

K

R

D

-

I

V

E

R

L

W

T

A

E

A

N

E

I

L

L

L

K

Q

V

I

M

-

N

-

L

-

E

E

Y

L

A

L

L

N

R

R

Y

Y

I

P

N

A

E

Q

L

L

S

S

G

G

E

Q

L

R

Q

Q

K

R

V

V

V

A

I

V

A

A

R

R

A

A

L

I

V

V

Q

V

E

E

P

P

Q

D

I

V

L

L

L

M

D

D

E

E

P

P

T

L

S

S

D

N

L

L

D

D

L

A

K

K

N

L

Q

R

L

V

E

A

V

M

M

R

K

A

I

E

I

I

R

K

E

K

V

L

S

Q

D

Q

T

K

H

L

N

K

I

V

A

T

S

T

I

I

V

Y

V

V

M

T

H

H

D

D

I

Q

N

V

L

E

A

A

L

M

R

T

Y

M

S

G

D

D

K

R

F

I

I

A

I

V

L

M

K

N

E

R

G

G

Q

Q

V

L

F

L

T

Q

T

I

G

G

binding adenosine-5'-triphosphate: F16 (≠ Y12), F19 (≠ A15), S41 (≠ N37), G42 (= G38), G44 (= G40), K45 (= K41), T46 (≠ S42), T47 (= T43)

5d3mA Folate ecf transporter: amppnp bound state (see paper)
29% identity, 90% coverage: 2:227/252 of query aligns to 5:234/280 of 5d3mA

query
sites
5d3mA

V

I

L

I

S

S

V

F

D

D

K

H

I

V

E

T

F
|
F

S

T

Y

Y

G

P

N

D

A

S

T

P

-

R

-

P

V

A

L

L

K

S

D

D

V

L

N

S

F

F

K

A

V

I

K

E

K

R

G

G

D

S

F

W

V

T

S

A

I

L

L

I

G

G

V

H

N
|
N

G
|
G

S

S

G

G

K
|
K

S
|
S

T

T

L

V

M

S

K

K

C

L

I

I

N

N

R

G

I

L

L

L

K

A

-

P

-

D

F

L

K

D

E

K

G

S

M

S

I

I

F

T

V

V

E

D

D

G

R

V

D

K

L

L

K

G

K

A

M

D

K

T

D

V

I

W

E

E

I

V

A

R

R

E

K

K

I

V

G

G

Y

I

V

V

P

F

Q

Q

S

N

S

P

E

D

T

N

G

Q

F

F

V

V

-

G

-

A

T

T

V

V

F

S

D

D

A

D

V

V

L

A

L

F

G

G

-

L

-

E

-

N

-

R

-

A

-

V

R

P

K

R

P

P

Y

E

I

M

K

-

W

-

D

-

I

-

S

-

K

-

D

-

I

L

E

K

L

I

T

V

E

A

N

Q

I

A

L

V

K

A

V

D

M

V

N

G

L

M

E

A

E

D

Y

Y

A

A

L

D

R

S

Y

E

I

P

N

S

E

N

L

L

S

S

G

G

E

Q

L

K

Q

Q

K

R

V

V

V

A

I

I

A

A

R

G

A

I

L

L

V

A

Q

V

E

K

P

P

Q

Q

I

V

L

I

L

L

D

D

E

E

P

S

T

T

S

S

D

M

L

L

D

D

L

P

K

E

N

G

Q

K

L

E

E

Q

V

I

M

L

K

D

I

L

I

V

R

R

E

K

V

I

S

K

D

E

T

D

H

N

N

N

I

L

A

T

S

V

I

I

V

S

V

I

M

T

H

H

D

D

I

L

N

E

L

E

A

A

L

-

R

A

Y

G

S

A

D

D

K

Q

F

V

I

L

I

V

L

L

K

D

E

D

G

G

Q

Q

V

L

F

L

T

D

T

Q

G

G

G

K

K

P

E

E

V

E

I

I

T

F

P

P

E

K

I

V

binding phosphoaminophosphonic acid-adenylate ester: F13 (= F10), N42 (= N37), G43 (= G38), K46 (= K41), S47 (= S42)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
32% identity, 87% coverage: 5:223/252 of query aligns to 4:223/240 of 4ymuJ

query
sites
4ymuJ

V

V

D

N

K

D

I

V

E

Y

F

K

S

N

Y
x
F

G

G

N

S

A

L

T

E

V
|
V

L

L

K

K

D

G

V

V

N

T

F

L

K

K

V

V

K

N

K

K

G

G

D

E

F

V

V

V

S

V

I

I

L

I

G

G

V

P

N
x
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

L

K

R

C

C

I

I

N

N

R

L

I

L

L

E

K

E

F

P

K

T

E

K

G

G

M

E

I

V

F

F

V

I

E

D

D

G

R

V

D

K

L

I

K

N

-

N

-

G

K

K

M

V

K

N

D

I

I

N

E

K

I

V

A

R

R

Q

K

K

I

V

G

G

Y

M

V

V

P

F

Q

Q

S

H

S

F

E

N

T

L

-

F

G

P

F

H

V

L

T

T

V

A

F

I

D

E

A

N

V

I

L

T

L

L

G

A

R

-

K

-

P

P

Y

V

I

K

K

V

W

K

D

K

I

M

S

N

K

K

D

E

I

A

-

E

E

E

L

L

T

A

E

V

N

D

I

L

L

L

K

A

V

K

M

V

N

G

L

L

E

L

E

D

Y

K

A

K

L

D

R

Q

Y

Y

I

P

N

I

E

K

L

L

S

S

G

G

E

Q

L

K

Q

Q

K

R

V

L

V

A

I

I

A

A

R

R

A

A

L

L

V

A

Q

M

E

Q

P

P

Q

E

I

V

L

M

L

L

L

F

D

D

E
|
E

P

P

T

T

S

S

D

A

L

L

D

D

L

P

K

E

N

M

Q

V

L

K

E

E

V

V

M

L

K

N

I

V

I

M

R

K

E

Q

V

L

S

A

D

N

T

-

H

E

N

G

I

M

A

T

S

M

I

V

V

V

M

T

H
|
H

D

E

I

M

N

G

L

F

A

A

L

R

R

E

Y

V

S

G

D

D

K

R

F

V

I

I

I

F

L

M

K

D

E

D

G

G

Q

V

V

I

F

V

T

E

G

G

G

T

K

P

E

E

V

E

I

I

binding adenosine-5'-triphosphate: F11 (≠ Y12), V16 (= V17), S36 (≠ N37), G37 (= G38), S38 (= S39), G39 (= G40), K40 (= K41), S41 (= S42), T42 (= T43), E162 (= E161), H194 (= H194)
binding magnesium ion: S41 (= S42), E162 (= E161)

8bmsA Cryo-em structure of the mutant solitary ecf module 2eq in msp2n2 lipid nanodiscs in the atpase closed and atp-bound conformation (see paper)
29% identity, 90% coverage: 2:227/252 of query aligns to 4:231/278 of 8bmsA

query
sites
8bmsA

V

I

L

I

S

S

V

F

D

D

K

H

I

V

E

T

F
|
F

S

T

Y

D

G

S

N

P

A

R

T

P

V

A

L

L

K

S

D

D

V

L

N

S

F

F

K

A

V

I

K

E

K

R

G

G

D

S

F

W

V

T

S

A

I

L

L

I

G

G

V

H

N
|
N

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

V

M

S

K

K

C

L

I

I

N

N

R

G

I

L

L

L

K

A

-

P

-

D

F

L

K

D

E

K

G

S

M

S

I

I

F

T

V

V

E

D

D

G

R

V

D

K

L

L

K

G

K

A

M

D

K

T

D

V

I

W

E

E

I

V

A

R

R

E

K

K

I

V

G

G

Y

I

V

V

P

F

Q
|
Q

S

N

S

P

E

D

T

N

G

Q

F

F

V

V

-

G

-

A

T

T

V

V

F

S

D

D

A

D

V

V

L

A

L

F

G

G

-

L

-

E

-

N

-

R

-

A

-

V

R

P

K

R

P

P

Y

E

I

M

K

-

W

-

D

-

I

-

S

-

K

-

D

-

I

L

E

K

L

I

T

V

E

A

N

Q

I

A

L

V

K

A

V

D

M

V

N

G

L

M

E

A

E

D

Y
|
Y

A

A

L

D

R

S

Y

E

I

P

N

S

E
x
N

L

L

S
|
S

G
|
G

E
x
Q

L

K

Q

Q

K

R

V

V

V

A

I

I

A

A

R

G

A

I

L

L

V

A

Q

V

E

K

P

P

Q

Q

I

V

L

I

L

L

D

D

E
x
Q

P

S

T

T

S

S

D

M

L

L

D

D

L

P

K

E

N

G

Q

K

L

E

E

Q

V

I

M

L

K

D

I

L

I

V

R

R

E

K

V

I

S

K

D

E

T

D

H

N

N

N

I

L

A

T

S

V

I

I

V

S

V

I

M

T

H
|
H

D

D

I

L

N

E

L

E

A

A

L

-

R

A

Y

G

S

A

D

D

K

Q

F

V

I

L

I

V

L

L

K

D

E

D

G

G

Q

Q

V

L

F

L

T

D

T

Q

G

G

G

K

K

P

E

E

V

E

I

I

T

F

P

P

E

K

I

V

binding adenosine-5'-triphosphate: F12 (= F10), N39 (= N37), G40 (= G38), S41 (= S39), G42 (= G40), K43 (= K41), S44 (= S42), T45 (= T43), Q88 (= Q83), Y133 (= Y128), N140 (≠ E135), S142 (= S137), G143 (= G138), G144 (= G139), Q145 (≠ E140), Q166 (≠ E161), H199 (= H194)
binding magnesium ion: S44 (= S42), Q88 (= Q83)

7arlD Lolcde in complex with lipoprotein and adp (see paper)
33% identity, 75% coverage: 17:206/252 of query aligns to 21:213/222 of 7arlD

query
sites
7arlD

V

V

L

L

K

H

D

N

V

V

N

S

F

F

K

S

V

V

K

G

K

E

G

G

D

E

F

M

V

M

S

A

I

I

L

V

G

G

V

S

N

S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T

T

L

L

M

L

K

H

C

L

I

L

N

G

R

G

I

L

L

D

K

T

F

P

K

T

E

S

G

G

M

D

I

V

F

I

V

F

E

N

D

G

R

Q

D

P

L

M

K

S

K

K

M

L

K

S

D

S

I

A

E

A

I

K

A

A

-

E

-

L

-

R

-

N

R

Q

K

K

I

L

G

G

Y

F

V

I

P

Y

Q

Q

S

F

S

H

E

H

-

L

-

P

-

D

-

F

T

T

G

A

F

L

V

E

T

N

V

V

F

A

D

M

A

P

V

L

L

L

L

I

G

G

R

K

K

K

P

K

Y

-

I

-

K

P

W

A

D

E

I

I

S

N

K

S

K

R

D

A

I

L

E

E

L

M

T

-

E

-

N

-

I

-

L

L

K

K

V

A

M

V

N

G

L

L

E

D

E

H

Y

R

A

A

L

N

R

H

Y

R

I

P

N

S

E

E

L

L

S

S

G

G

E

E

L

R

Q

Q

K

R

V

V

V

A

I

I

A

A

R

R

A

A

L

L

V

V

Q

N

E

N

P

P

Q

R

I

L

L

V

L

L

L

A

D

D

E

E

P

P

T

T

S

G

D

N

L

L

D

D

L

A

K

R

N

N

Q

A

L

D

E

S

V

I

M

F

K

Q

I

L

R

G

E

E

V

L

S

N

D

R

T

L

H

Q

N

G

I

T

A

A

S

F

I

L

V

V

M

T

H

H

D

D

I

L

N

Q

L

L

A

A

L

K

R

R

Y

M

S

S

D

R

K

Q

F

L

binding adenosine-5'-diphosphate: G42 (= G38), G44 (= G40), K45 (= K41), S46 (= S42)

P75957 Lipoprotein-releasing system ATP-binding protein LolD; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
33% identity, 75% coverage: 17:206/252 of query aligns to 24:216/233 of P75957

query
sites
P75957

V

V

L

L

K

H

D

N

V

V

N

S

F

F

K

S

V

V

K

G

K

E

G

G

D

E

F

M

V

M

S

A

I

I

L

V

G
|
G

V

S

N

S

G

G

S

S

G

G

K

K

S

S

T

T

L

L

M

L

K

H

C

L

I

L

N

G

R

G

I

L

L

D

K

T

F

P

K

T

E

S

G

G

M

D

I

V

F

I

V

F

E

N

D

G

R

Q

D

P

L

M

K

S

K

K

M

L

K

S

D

S

I

A

E

A

I

K

A

A

-

E

-

L

-

R

-

N

R

Q

K

K

I

L

G

G

Y

F

V

I

P

Y

Q

Q

S

F

S

H

E

H

-

L

-

P

-

D

-

F

T

T

G

A

F

L

V

E

T

N

V

V

F

A

D

M

A

P

V

L

L

L

L

I

G

G

R

K

K

K

P

K

Y

-

I

-

K

P

W

A

D

E

I

I

S

N

K

S

K

R

D

A

I

L

E

E

L

M

T

-

E

-

N

-

I

-

L

L

K

K

V

A

M

V

N

G

L

L

E

D

E

H

Y

R

A

A

L

N

R

H

Y

R

I

P

N

S

E

E

L

L

S

S

G

G

E

E

L

R

Q

Q

K

R

V

V

V

A

I

I

A

A

R

R

A

A

L

L

V

V

Q

N

E

N

P

P

Q

R

I

L

L

V

L

L

L

A

D

D

E

E

P

P

T

T

S

G

D

N

L

L

D

D

L

A

K

R

N

N

Q

A

L

D

E

S

V

I

M

F

K

Q

I

L

R

G

E

E

V

L

S

N

D

R

T

L

H

Q

N

G

I

T

A

A

S

F

I

L

V

V

M

T

H

H

D

D

I

L

N

Q

L

L

A

A

L

K

R

R

Y

M

S

S

D

R

K

Q

F

L

G42 (= G35) mutation to D: Loss of lipoprotein release when overexpressed.

Q5M243 Energy-coupling factor transporter ATP-binding protein EcfA1; ECF transporter A component EcfA1; EC 7.-.-.- from Streptococcus thermophilus (strain ATCC BAA-250 / LMG 18311) (see paper)
32% identity, 85% coverage: 2:214/252 of query aligns to 1:215/276 of Q5M243

query
sites
Q5M243

V

M

L

I

S

E

V

I

D

K

K

N

I

L

E

K

F

F

S

K

Y

Y

G

N

N

Q

-

D

-

Q

-

T

A

S

T

Y

V

T

L

L

K

N

D

D

V

V

N

S

F

F

K

H

V

V

K

K

K

H

G

G

D

E

F

W

V

L

S

S

I

I

L

V

G

G

V

H

N

N

G

G

S

S

G

G

K

K

S

S

T

T

L

T

M

A

K

R

C

L

I

I

N

G

R

G

I

L

L

L

K

V

F

A

K

D

E

S

G

G

M

Q

I

I

F

I

V

V

E

D

D

G

R

Q

D

E

L

L

K

T

K

E

M

E

K

T

D

V

I

W

E

D

I

I

A

R

R

D

K

K

I

I

G

G

Y

M

V

V

P

F

Q

Q

S

N

S

P

E

D

T

N

G
x
Q

F

F

V

V

-

G

-

A

T

T

V

V

F

E

D

D

A

D

V

V

L

A

L

F

G

G

R

L

K

E

-

N

-

K

-

G

-

L

P

P

Y

Y

I

-

K

-

W

-

D

-

I

-

S

-

K

K

K

E

D

M

I

V

E

S

L

R

T

V

E

Q

N

E

I

A

L

L

K

S

V

F

M

V

N

G

L

M

E

M

E

D

Y

F

A

K

L

D

R

R

Y

E

I

P

N

A

E

R

L

L

S

S

G

G

E

Q

L

K

Q

Q

K

R

V

V

V

A

I

I

A

A

R

G

A

I

L

I

V

A

Q

M

E

R

P

P

Q

S

I

I

L

L

L

I

L

L

D

D

E
|
E

P

A

T

T

S

S

D

M

L

L

D

D

L

P

K

E

N

G

Q

R

L

Q

E

E

V

L

M

I

K

Q

I

Y

I

I

R

E

E

D

V

I

S

R

D

Q

T

Q

H

Y

N

G

I

M

A

T

S

V

I

L

V

S

V

I

M

T

H

H

D

D

I

L

N

D

L

-

A

E

L

V

R

A

Y

M

S

S

D

N

K

R

F

V

I

L

I

V

L

L

K

K

E

Q

G

G

Q

K

V

V

Q90 (≠ G88) mutation to A: No effect on ATPase, 10-fold decrease in riboflavin uptake; when associated with A-97 in EcfA2.
E163 (= E161) mutation to Q: 10-fold decrease in ATPase and riboflavin uptake; when associated with Q-171 in EcfA2.

7mdyC Lolcde nucleotide-bound
33% identity, 75% coverage: 17:206/252 of query aligns to 21:213/226 of 7mdyC

query
sites
7mdyC

V

V

L

L

K

H

D

N

V

V

N

S

F

F

K

S

V

V

K

G

K

E

G

G

D

E

F

M

V

M

S

A

I

I

L

V

G

G

V

S

N

S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

L

K

H

C

L

I

L

N

G

R

G

I

L

L

D

K

T

F

P

K

T

E

S

G

G

M

D

I

V

F

I

V

F

E

N

D

G

R

Q

D

P

L

M

K

S

K

K

M

L

K

S

D

S

I

A

E

A

I

K

A

A

-

E

-

L

-

R

-

N

R

Q

K

K

I

L

G

G

Y

F

V

I

P

Y

Q
|
Q

S

F

S

H

E

H

-

L

-

P

-

D

-

F

T

T

G

A

F

L

V

E

T

N

V

V

F

A

D

M

A

P

V

L

L

L

L

I

G

G

R

K

K

K

P

K

Y

-

I

-

K

P

W

A

D

E

I

I

S

N

K

S

K

R

D

A

I

L

E

E

L

M

T

-

E

-

N

-

I

-

L

L

K

K

V

A

M

V

N

G

L

L

E

D

E

H

Y

R

A

A

L

N

R
x
H

Y

R

I

P

N

S

E
|
E

L

L

S
|
S

G
|
G

E

E

L

R

Q

Q

K

R

V

V

V

A

I

I

A

A

R

R

A

A

L

L

V

V

Q

N

E

N

P

P

Q

R

I

L

L

V

L

L

L

A

D

D

E
|
E

P

P

T

T

S

G

D
x
N

L

L

D

D

L

A

K

R

N

N

Q

A

L

D

E

S

V

I

M

F

K

Q

I

L

R

G

E

E

V

L

S

N

D

R

T

L

H

Q

N

G

I

T

A

A

S

F

I

L

V

V

M

T

H
|
H

D

D

I

L

N

Q

L

L

A

A

L

K

R

R

Y

M

S

S

D

R

K

Q

F

L

binding adp orthovanadate: G42 (= G38), S43 (= S39), G44 (= G40), K45 (= K41), S46 (= S42), T47 (= T43), Q91 (= Q83), H138 (≠ R131), E142 (= E135), S144 (= S137), G145 (= G138), G146 (= G139), E168 (= E161), N172 (≠ D165), H201 (= H194)
binding magnesium ion: S46 (= S42), Q91 (= Q83)

Sites not aligning to the query:

binding adp orthovanadate: 12

2pclA Crystal structure of abc transporter with complex (aq_297) from aquifex aeolicus vf5
35% identity, 79% coverage: 17:214/252 of query aligns to 18:216/223 of 2pclA

query
sites
2pclA

V

I

L

L

K

K

D

G

V

I

N

S

F

L

K

S

V

V

K

K

G

G

D

E

F

F

V

V

S

S

I

I

L

I

G

G

V

A

N

S

G

G

S

S

G

G

K

K

S

S

T

T

L

L

M

L

K

Y

C

I

I

L

N

G

R

L

I

L

L

D

K

A

F

P

K

T

E

E

G

G

M

K

I

V

F

F

V

L

E

E

D

G

R

K

D

E

L

V

K

D

K

Y

M

T

K

N

D

E

I

K

E

E

I

L

A

S

-

L

-

R

-

N

R

R

K

K

I

L

G

G

Y

F

V

V

P

F

Q

Q

S

F

S

H

E

Y

-

L

T

I

G

P

F

E

V

L

T

T

V

A

F

L

D

E

A

N

V

V

L

I

L

V

G

-

R

-

K

-

P

P

Y

M

I

L

K

K

W

M

D

G

I

K

S

P

K

K

D

E

I

A

-

K

E

E

L

R

T

G

E

E

N

Y

I

L

L

L

K

S

V

E

M

L

N

G

L

L

E

G

E

D

Y

K

A

L

L

S

R

R

Y

K

I

P

N

Y

E

E

L

L

S

S

G

G

E

E

L

Q

Q

Q

K

R

V

V

V

A

I

I

A

A

R

R

A

A

L

L

V

A

Q

N

E

E

P

P

Q

I

I

L

L

L

L

F

L

A

D

D

E
|
E

P
|
P

T
|
T

S

G

D

N

L

L

D

D

L

S

K

A

N

N

Q

T

L

K

E

R

V

V

M

M

K

D

I

I

I

F

R

L

E

K

V

I

S

N

D

E

T

G

H

-

N

G

I

T

A

S

S

I

I

V

V

M

V

V

M
x
T

H

H

D

E

I

R

N

E

L

L

A

A

L

-

R

E

Y

L

S

T

D

H

K

R

F

T

I

L

I

E

L

M

K

K

E

D

G

G

Q

K

V

V

binding magnesium ion: E165 (= E161), P166 (= P162), T167 (= T163), T196 (≠ M193)

7ahhC Opua inhibited inward-facing, sbd docked (see paper)
32% identity, 81% coverage: 20:224/252 of query aligns to 44:252/382 of 7ahhC

query
sites
7ahhC

D

D

V

T

N

N

F

F

K

E

V

I

K

N

K

E

G

G

D

E

F

I

V

F

S

V

I

I

L

M

G

G

V

L

N

S

G
|
G

S

S

G
|
G

K
|
K

S

S

T

T

L

L

M

L

K

R

C

L

I

L

N

N

R

R

I

L

L

I

K

E

F

P

K

T

E

S

G

G

M

K

I

I

F

F

V

I

E

D

D

N

R

Q

D

D

-

V

-

A

-

T

L

L

K

N

K

K

M

E

K

D

D

L

I

L

E

Q

I

V

A

R

R

R

K

K

-

T

-

M

-

S

-

M

-

V

-

F

-

Q

-

N

I

F

G

G

Y

L

V

F

P

P

Q

H

S

R

S

-

E

-

T

-

G

-

F

-

V

-

T

T

V

I

F

L

D

E

A

N

V

T

L

E

L

Y

G

G

R

L

K

E

P

-

Y

-

I

V

K

Q

W

N

D

V

I

P

S

K

K

E

D

R

I

R

E

K

L

R

T

A

E

E

N

K

I

A

L

L

K

D

V

N

M

A

N

N

L

L

E

L

E

D

Y

F

A

K

L

D

R

Q

Y

Y

I

P

N

K

E

Q

L

L

S

S

G

G

E

M

L

Q

Q

Q

K

R

V

V

V

G

I

L

A

A

R

R

A

A

L

L

V

A

Q

N

E

D

P

P

Q

E

I

I

L

L

L

M

D
|
D

E
|
E

P

A

T

F

S

S

D

A

L

L

D

D

L

P

K

L

N

I

Q

R

L

R

E

E

V

M

M

Q

K

D

I

E

I

L

R

L

E

E

V

L

S

Q

D

A

T

K

H

F

N

Q

I

K

A

T

S

I

I

I

V

F

V

V

M

S

H

H

D

D

I

L

N

N

L

E

A

A

L

L

R

R

Y

I

S

G

D

D

K

R

F

I

I

A

I

I

L

M

K

K

E

D

G

G

Q

K

V

I

F

M

T

Q

T

I

G

G

-

T

G

G

K

E

E

E

V

I

I

L

T

T

binding phosphoaminophosphonic acid-adenylate ester: G62 (= G38), G64 (= G40), K65 (= K41), D187 (= D160), E188 (= E161)

Sites not aligning to the query:

Query Sequence

>WP_048081501.1 NCBI__GCF_000746075.1:WP_048081501.1
MVLSVDKIEFSYGNATVLKDVNFKVKKGDFVSILGVNGSGKSTLMKCINRILKFKEGMIF
VEDRDLKKMKDIEIARKIGYVPQSSETGFVTVFDAVLLGRKPYIKWDISKKDIELTENIL
KVMNLEEYALRYINELSGGELQKVVIARALVQEPQILLLDEPTSDLDLKNQLEVMKIIRE
VSDTHNIASIVVMHDINLALRYSDKFIILKEGQVFTTGGKEVITPEIIKETYGVDVHVKN
FEGVPMVIPKSQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory