SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_048081634.1 NCBI__GCF_000746075.1:WP_048081634.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
52% identity, 96% coverage: 6:224/229 of query aligns to 1:227/232 of 1f3oA

query
sites
1f3oA

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binding adenosine-5'-diphosphate: Y11 (= Y16), G41 (= G44), G43 (= G46), K44 (= K47), S45 (= S48), T46 (= T49)

1l2tA Dimeric structure of mj0796, a bacterial abc transporter cassette (see paper)
52% identity, 96% coverage: 6:224/229 of query aligns to 1:227/230 of 1l2tA

query
sites
1l2tA

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binding adenosine-5'-triphosphate: Y11 (= Y16), S40 (= S43), G41 (= G44), S42 (= S45), G43 (= G46), K44 (= K47), S45 (= S48), T46 (= T49), F138 (≠ I136), Q145 (≠ K142), S147 (= S144), G149 (= G146), Q150 (≠ E147), H204 (= H201)

5xu1B Structure of a non-canonical abc transporter from streptococcus pneumoniae r6 (see paper)
52% identity, 95% coverage: 4:220/229 of query aligns to 1:220/226 of 5xu1B

query
sites
5xu1B

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binding magnesium ion: G45 (= G46), K46 (= K47), S47 (= S48)

5lj7A Structure of aggregatibacter actinomycetemcomitans macb bound to atp (p21) (see paper)
47% identity, 96% coverage: 5:223/229 of query aligns to 2:223/592 of 5lj7A

query
sites
5lj7A

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binding adenosine-5'-triphosphate: V22 (≠ A23), S42 (= S43), G43 (= G44), G45 (= G46), K46 (= K47), S47 (= S48), T48 (= T49), Q92 (= Q91), K136 (≠ R135), Q143 (≠ K142), S145 (= S144), G147 (= G146), Q148 (≠ E147)
binding magnesium ion: S47 (= S48), Q92 (= Q91)

5lilA Structure of aggregatibacter actinomycetemcomitans macb bound to atpys (p21) (see paper)
47% identity, 96% coverage: 5:223/229 of query aligns to 2:223/615 of 5lilA

query
sites
5lilA

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binding adenosine-5'-triphosphate: F13 (≠ Y16), V22 (≠ A23), S42 (= S43), G43 (= G44), G45 (= G46), K46 (= K47), S47 (= S48), T48 (= T49), Q92 (= Q91), K136 (≠ R135), Q143 (≠ K142), S145 (= S144), G147 (= G146), Q148 (≠ E147)
binding magnesium ion: S47 (= S48), Q92 (= Q91)

P75831 Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
44% identity, 94% coverage: 6:221/229 of query aligns to 4:222/648 of P75831

query
sites
P75831

I

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Q

F

L

R

R

S

R

Q

E

E

H

I

F

G

G

F

F

V

I

F

F

Q

Q

L

R

H

Y

N

H

L

L

I

L

P

S

N

H

L

L

T

T

V

A

L

E

E

Q

N

N

V

V

Q

E

I

V

P

P

M

A

I

V

G

Y

T

A

S

G

I

L

S

E

G

R

K

K

D

Q

M

R

E

L

K

L

R

R

A

A

L

Q

K

E

L

L

K

Q

S

R

V

L

D

G

L

L

E

E

D

D

R

R

I

T

D

E

Q

Y

R

Y

P

P

T

A

K

Q

L

L

S

S

G

G

E

Q

R

Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

A

A

L

L

V

M

N

N

N

G

P

G

S

Q

I

V

I

I

L

L

A

A

D
|
D

E

E

P

P

T

T

G

G

A

A

L

L

D

D

S

S

K

H

T

S

G

G

D

E

I

E

I

V

L

M

D

A

L

I

L

L

K

H

D

Q

L

L

H

R

K

D

K

R

E

G

N

H

V

-

T

T

L

V

V

I

M

I

V

V

T

T

H

H

E

D

P

P

Y

Q

V

V

A

A

N

A

M

Q

A

A

D

E

R

R

I

V

I

I

T

E

V

I

R

R

D

D

G

G

K

E

I

I

L

V

K47 (= K47) mutation to L: Lack of activity.
D169 (= D167) mutation to N: Lack of activity.

8g4cB Bceabs atpgs high res tm (see paper)
43% identity, 95% coverage: 6:223/229 of query aligns to 3:224/248 of 8g4cB

query
sites
8g4cB

I

I

I

L

E

E

I

A

K

N

G

K

L

I

K

R

K

K

S

S

Y
|
Y

D

G

K

N

-

K

-

L

G

N

K

K

I

Q

K

E

A

V

L

L

N

K

G

G

I

I

D

D

L

I

E

H

I

I

R

E

K

K

G

G

E

E

F

F

V

V

S

S

I

I

I

M

G

G

P

A

S
|
S

G

G

S
|
S

G

G

K

K

S
x
T

T

T

L

L

N

N

M

V

I

L

G

S

A

S

L

I

D

D

T

Q

A

V

G

S

E

H

G

G

S

T

V

I

E

H

V

I

A

N

G

G

I

N

D

D

L

M

T

T

-

A

-

M

K

K

T

E

K

K

D

Q

L

L

S

A

K

E

F

F

R

R

S

K

Q

Q

E

H

I

L

G

G

F

F

V

I

F

F

Q

Q

L

D

H

Y

N

N

L

L

I

L

P

D

N

T

L

L

T

T

V

V

L

K

E

E

N

N

V

I

Q

L

I

L

P

P

M

L

I

S

G

I

T

T

S

K

I

L

S

S

G

K

K

K

D

E

M

A

E

N

K

R

R

K

A

F

L

E

K

E

L

V

L

A

K

K

S

E

V

L

D

G

L

I

E

Y

D

E

R

L

I

R

D

D

Q

K

R

Y

P

P

T

N

K

E

L

I

S

S

G

G

E

Q

R

K

Q

Q

R

R

V

T

A

S

I

A

A

G

R

R

A

A

L

F

V

I

N

H

N

D

P

P

S

S

I

I

L

F

A

A

D
|
D

E

E

P

P

T

T

G

G

A

A

L

L

D

D

S

S

K

K

T

S

G

A

D

S

I

D

I

L

L

L

D

N

L

K

L

L

K

S

D

Q

L

L

H

N

K

Q

K

K

E

R

N

N

V

A

T

T

L

I

V

I

M

M

V

V

T

T

H

H

E

D

P

P

Y

V

V

A

A

A

N

S

M

Y

A

C

D

G

R

R

I

V

I

I

T

F

V

I

R

K

D

D

G

G

K

Q

I

M

L

Y

E

T

E

Q

binding phosphothiophosphoric acid-adenylate ester: Y13 (= Y16), S42 (= S43), S44 (= S45), T47 (≠ S48), D168 (= D167)

7tchB Bceab e169q variant atp-bound conformation (see paper)
42% identity, 95% coverage: 6:223/229 of query aligns to 2:223/245 of 7tchB

query
sites
7tchB

I

I

I

L

E

E

I

A

K

N

G

K

L

I

K

R

K

K

S

S

Y
|
Y

D

G

K

N

-

K

-

L

G

N

K

K

I
x
Q

K

E

A
x
V

L

L

N

K

G

G

I

I

D

D

L

I

E

H

I

I

R

E

K

K

G

G

E

E

F

F

V

V

S

S

I

I

I

M

G

G

P

A

S
|
S

G
|
G

S
|
S

G

G

K
|
K

S
x
T

T

T

L

L

N

N

M

V

I

L

G

S

A

S

L

I

D

D

T

Q

A

V

G

S

E

H

G

G

S

T

V

I

E

H

V

I

A

N

G

G

I

N

D

D

L

M

T

T

-

A

-

M

K

K

T

E

K

K

D

Q

L

L

S

A

K

E

F

F

R

R

S

K

Q

Q

E

H

I

L

G

G

F

F

V

I

F

F

Q

Q

L

D

H

Y

N

N

L

L

I

L

P

D

N

T

L

L

T

T

V

V

L

K

E

E

N

N

V

I

Q

L

I

L

P

P

M

L

I

S

G

I

T

T

S

K

I

L

S

S

G

K

K

K

D

E

M

A

E

N

K

R

R

K

A

F

L

E

K

E

L

V

L

A

K

K

S

E

V

L

D

G

L

I

E

Y

D

E

R

L

I

R

D

D

Q

K

R

Y

P

P

T

N

K
x
E

L

I

S

S

G
|
G

E

Q

R

K

Q

Q

R

R

V

T

A

S

I

A

A

G

R

R

A

A

L

F

V

I

N

H

N

D

P

P

S

S

I

I

L

F

A

A

D

D

E

Q

P

P

T

T

G

G

A

A

L

L

D

D

S

S

K

K

T

S

G

A

D

S

I

D

I

L

L

L

D

N

L

K

L

L

K

S

D

Q

L

L

H

N

K

Q

K

K

E

R

N

N

V

A

T

T

L

I

V

I

M

M

V

V

T

T

H

H

E

D

P

P

Y

V

V

A

A

A

N

S

M

Y

A

C

D

G

R

R

I

V

I

I

T

F

V

I

R

K

D

D

G

G

K

Q

I

M

L

Y

E

T

E

Q

binding adenosine-5'-triphosphate: Y12 (= Y16), Q19 (≠ I21), V21 (≠ A23), S41 (= S43), G42 (= G44), S43 (= S45), K45 (= K47), T46 (≠ S48), E142 (≠ K142), G145 (= G145), G146 (= G146)

P75957 Lipoprotein-releasing system ATP-binding protein LolD; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
42% identity, 97% coverage: 3:223/229 of query aligns to 2:226/233 of P75957

query
sites
P75957

N

N

E

K

N

I

I

L

E

Q

I

C

K

D

G

N

L

L

K

C

K

K

S

R

Y

Y

D

Q

K

E

G

G

K

S

I

V

K

Q

A

T

-

D

-

V

L

L

N

H

G

N

I

V

D

S

L

F

E

S

I

V

R

G

K

E

G

G

E

E

F

M

V

M

S

A

I

I

I

V

G
|
G

P

S

S

S

G

G

S

S

G

G

K

K

S

S

T

T

L

L

N

H

M

L

I

L

G

G

A

G

L

L

D

D

T

T

A

P

G

T

E

S

G

G

S

D

V

V

E

I

V

F

A

N

G

G

I

Q

D

P

L

M

T

S

K

K

T

L

K

S

D

S

L

A

S

A

K

K

-

A

-

E

F

L

R

R

S

N

Q

Q

E

K

I

L

G

G

F

F

V

I

F

Y

Q

Q

L

F

H

H

N

H

L

L

I

L

P

P

N

D

L

F

T

T

V

A

L

L

E

E

N

N

V

V

Q

A

I

M

P

P

M

L

I

L

G

I

T

G

S

K

I

K

S

K

G

P

K

A

D

E

M

I

E

N

K

S

R

R

A

A

L

L

K

E

L

M

L

L

K

K

S

A

V

V

D

G

L

L

E

D

D

H

R

R

I

A

D

N

Q

H

R

R

P

P

T

S

K

E

L

L

S

S

G

G

E

E

R

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

V

V

N

N

P

P

S

R

I

L

I

V

L

L

A

A

D

D

E

E

P

P

T

T

G

G

A

N

L

L

D

D

S

A

K

R

T

N

G

A

D

D

I

S

I

I

L

F

D

Q

L

L

K

G

D

E

L

L

H

N

K

R

K

L

E

Q

N

G

V

T

T

A

L

F

V

L

M

V

V

V

T

T

H

H

E

D

P

L

Y

Q

V

L

A

A

N

K

M

R

A

M

D

S

R

R

I

Q

I

L

T

E

V

M

R

R

D

D

G

G

K

R

I

L

L

T

E

A

E

E

G42 (= G41) mutation to D: Loss of lipoprotein release when overexpressed.

7mdyC Lolcde nucleotide-bound
42% identity, 95% coverage: 6:223/229 of query aligns to 2:223/226 of 7mdyC

query
sites
7mdyC

I

L

E

Q

I

C

K

D

G

N

L

L

K

C

K

K

S

R

Y
|
Y

D

Q

K

E

G

G

K

S

I

V

K

Q

A

T

-

D

-

V

L

L

N

H

G

N

I

V

D

S

L

F

E

S

I

V

R

G

K

E

G

G

E

E

F

M

V

M

S

A

I

I

I

V

G

G

P

S

S

S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

N

H

M

L

I

L

G

G

A

G

L

L

D

D

T

T

A

P

G

T

E

S

G

G

S

D

V

V

E

I

V

F

A

N

G

G

I

Q

D

P

L

M

T

S

K

K

T

L

K

S

D

S

L

A

S

A

K

K

-

A

-

E

F

L

R

R

S

N

Q

Q

E

K

I

L

G

G

F

F

V

I

F

Y

Q
|
Q

L

F

H

H

N

H

L

L

I

L

P

P

N

D

L

F

T

T

V

A

L

L

E

E

N

N

V

V

Q

A

I

M

P

P

M

L

I

L

G

I

T

G

S

K

I

K

S

K

G

P

K

A

D

E

M

I

E

N

K

S

R

R

A

A

L

L

K

E

L

M

L

L

K

K

S

A

V

V

D

G

L

L

E

D

D

H

R

R

I

A

D

N

Q
x
H

R

R

P

P

T

S

K
x
E

L

L

S
|
S

G
|
G

E

E

R

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

V

V

N

N

P

P

S

R

I

L

I

V

L

L

A

A

D

D

E
|
E

P

P

T

T

G

G

A
x
N

L

L

D

D

S

A

K

R

T

N

G

A

D

D

I

S

I

I

L

F

D

Q

L

L

K

G

D

E

L

L

H

N

K

R

K

L

E

Q

N

G

V

T

T

A

L

F

V

L

M

V

V

V

T

T

H
|
H

E

D

P

L

Y

Q

V

L

A

A

N

K

M

R

A

M

D

S

R

R

I

Q

I

L

T

E

V

M

R

R

D

D

G

G

K

R

I

L

L

T

E

A

E

E

binding adp orthovanadate: Y12 (= Y16), G42 (= G44), S43 (= S45), G44 (= G46), K45 (= K47), S46 (= S48), T47 (= T49), Q91 (= Q91), H138 (≠ Q138), E142 (≠ K142), S144 (= S144), G145 (= G145), G146 (= G146), E168 (= E168), N172 (≠ A172), H201 (= H201)
binding magnesium ion: S46 (= S48), Q91 (= Q91)

7v8iD Lolcd(e171q)e with bound amppnp in nanodiscs (see paper)
41% identity, 97% coverage: 3:223/229 of query aligns to 1:225/229 of 7v8iD

query
sites
7v8iD

N

N

E

K

N

I

I

L

E

Q

I

C

K

D

G

N

L

L

K

C

K

K

S

R

Y

Y

D

Q

K

E

G

G

K

S

I

V

K

Q

A

T

-

D

-
x
V

L

L

N

H

G

N

I

V

D

S

L

F

E

S

I

V

R

G

K

E

G

G

E

E

F

M

V

M

S

A

I

I

I

V

G

G

P

S

S
|
S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

N

H

M

L

I

L

G

G

A

G

L

L

D

D

T

T

A

P

G

T

E

S

G

G

S

D

V

V

E

I

V

F

A

N

G

G

I

Q

D

P

L

M

T

S

K

K

T

L

K

S

D

S

L

A

S

A

K

K

-

A

-

E

F

L

R

R

S

N

Q

Q

E

K

I

L

G

G

F

F

V

I

F

Y

Q
|
Q

L

F

H

H

N

H

L

L

I

L

P

P

N

D

L

F

T

T

V

A

L

L

E

E

N

N

V

V

Q

A

I

M

P

P

M

L

I

L

G

I

T

G

S

K

I

K

S

K

G

P

K

A

D

E

M

I

E

N

K

S

R

R

A

A

L

L

K

E

L

M

L

L

K

K

S

A

V

V

D

G

L

L

E

D

D

H

R
|
R

I

A

D

N

Q
x
H

R

R

P

P

T

S

K
x
E

L

L

S
|
S

G

G

G
|
G

E
|
E

R

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

V

V

N

N

P

P

S

R

I

L

I

V

L

L

A

A

D

D

E

Q

P

P

T

T

G

G

A

N

L

L

D

D

S

A

K

R

T

N

G

A

D

D

I

S

I

I

L

F

D

Q

L

L

K

G

D

E

L

L

H

N

K

R

K

L

E

Q

N

G

V

T

T

A

L

F

V

L

M

V

V

V

T

T

H
|
H

E

D

P

L

Y

Q

V

L

A

A

N

K

M

R

A

M

D

S

R

R

I

Q

I

L

T

E

V

M

R

R

D

D

G

G

K

R

I

L

L

T

E

A

E

E

binding phosphoaminophosphonic acid-adenylate ester: V23 (vs. gap), S43 (= S43), G44 (= G44), G46 (= G46), K47 (= K47), S48 (= S48), T49 (= T49), Q93 (= Q91), R137 (= R135), H140 (≠ Q138), E144 (≠ K142), S146 (= S144), G148 (= G146), E149 (= E147), H203 (= H201)
binding magnesium ion: S48 (= S48), Q93 (= Q91)

7arlD Lolcde in complex with lipoprotein and adp (see paper)
42% identity, 94% coverage: 6:220/229 of query aligns to 2:220/222 of 7arlD

query
sites
7arlD

I

L

E

Q

I

C

K

D

G

N

L

L

K

C

K

K

S

R

Y

Y

D

Q

K

E

G

G

K

S

I

V

K

Q

A

T

-

D

-

V

L

L

N

H

G

N

I

V

D

S

L

F

E

S

I

V

R

G

K

E

G

G

E

E

F

M

V

M

S

A

I

I

I

V

G

G

P

S

S

S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T

T

L

L

N

H

M

L

I

L

G

G

A

G

L

L

D

D

T

T

A

P

G

T

E

S

G

G

S

D

V

V

E

I

V

F

A

N

G

G

I

Q

D

P

L

M

T

S

K

K

T

L

K

S

D

S

L

A

S

A

K

K

-

A

-

E

F

L

R

R

S

N

Q

Q

E

K

I

L

G

G

F

F

V

I

F

Y

Q

Q

L

F

H

H

N

H

L

L

I

L

P

P

N

D

L

F

T

T

V

A

L

L

E

E

N

N

V

V

Q

A

I

M

P

P

M

L

I

L

G

I

T

G

S

K

I

K

S

K

G

P

K

A

D

E

M

I

E

N

K

S

R

R

A

A

L

L

K

E

L

M

L

L

K

K

S

A

V

V

D

G

L

L

E

D

D

H

R

R

I

A

D

N

Q

H

R

R

P

P

T

S

K

E

L

L

S

S

G

G

E

E

R

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

V

V

N

N

P

P

S

R

I

L

I

V

L

L

A

A

D

D

E

E

P

P

T

T

G

G

A

N

L

L

D

D

S

A

K

R

T

N

G

A

D

D

I

S

I

I

L

F

D

Q

L

L

K

G

D

E

L

L

H

N

K

R

K

L

E

Q

N

G

V

T

T

A

L

F

V

L

M

V

V

V

T

T

H

H

E

D

P

L

Y

Q

V

L

A

A

N

K

M

R

A

M

D

S

R

R

I

Q

I

L

T

E

V

M

R

R

D

D

G

G

K

R

I

L

binding adenosine-5'-diphosphate: G42 (= G44), G44 (= G46), K45 (= K47), S46 (= S48)

2pclA Crystal structure of abc transporter with complex (aq_297) from aquifex aeolicus vf5
48% identity, 88% coverage: 24:225/229 of query aligns to 19:221/223 of 2pclA

query
sites
2pclA

L

L

N

K

G

G

I

I

D

S

L

L

E

S

I

V

R

K

K

K

G

G

E

E

F

F

V

V

S

S

I

I

G

G

P

A

S

S

G

G

S

S

G

G

K

K

S

S

T

T

L

L

N

Y

M

I

I

L

G

G

A

L

L

L

D

D

T

A

A

P

G

T

E

E

G

G

S

K

V

V

E

F

V

L

A

E

G

G

-

K

-

E

I

V

D

D

L

Y

T

T

K

N

T

E

K

K

D

E

L

L

S

S

K

L

F

L

R

R

S

N

Q

R

E

K

I

L

G

G

F

F

V

V

F

F

Q

Q

L

F

H

H

N

Y

L

L

I

I

P

P

N

E

L

L

T

T

V

A

L

L

E

E

N

N

V

V

Q

I

I

V

P

P

M

M

I

L

G

K

T

M

S

G

I

K

S

P

G

K

K

K

D

E

M

A

E

K

K

E

R

R

A

G

L

E

K

Y

L

L

K

S

S

E

V

L

D

G

L

L

E

G

D

D

R

K

I

L

D

S

Q

R

R

K

P

P

T

Y

K

E

L

L

S

S

G

G

E

E

R

Q

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

V

A

N

N

N

E

P

P

S

I

I

L

L

F

A

A

D

D

E
|
E

P
|
P

T
|
T

G

G

A

N

L

L

D

D

S

S

K

A

T

N

G

T

D

K

I

R

I

V

L

M

D

D

L

I

L

F

K

L

D

K

L

I

H

N

K

E

K

G

E

-

N

G

V

T

T

S

L

I

V

V

M

M

V

V

T
|
T

H

H

E

E

P

R

Y

E

V

L

A

A

N

E

M

L

A

T

D

H

R

R

I

T

I

L

T

E

V

M

R

K

D

D

G

G

K

K

I

V

L

V

E

G

E

E

K

I

T

T

binding magnesium ion: E165 (= E168), P166 (= P169), T167 (= T170), T196 (= T200)

5ws4A Crystal structure of tripartite-type abc transporter macb from acinetobacter baumannii (see paper)
41% identity, 97% coverage: 4:226/229 of query aligns to 2:227/650 of 5ws4A

query
sites
5ws4A

E

Q

N

A

I

L

E

E

I

V

K

S

G

N

L

L

K

V

K

R

S

E

Y

F

D

P

K

A

G

G

K

E

-

S

-

T

I

I

K

Q

A

I

L

L

N

K

G

G

I

I

D

D

L

L

E

T

I

I

R

Y

K

E

G

G

E

E

F

L

V

V

S

A

I

I

I

V

G

G

P

Q

S
|
S

G
|
G

S

S

G

G

K

K

S
|
S

T

T

L

L

L

M

N

N

M

I

I

L

G

G

A

C

L

L

D

D

T

R

A

P

G

T

E

S

G

G

S

S

V

Y

E

K

V

V

A

N

G

G

I

Q

D

E

L

T

T

G

K

K

T

L

K

E

-

P

-

D

D

Q

L

L

S

A

K

Q

F

L

R

R

S

R

Q

E

E

Y

I

F

G

G

F

F

V

I

F

F

Q

Q

L

R

H

Y

N

H

L

L

I

L

P

G

N

D

L

L

T

S

V

A

L

E

E

G

N

N

V

V

Q

E

I

V

P

P

M

A

I

V

G

Y

T

A

S

G

I

V

S

T

G

P

K

A

D

D

M

R

E

K

K

Q

R

R

A

A

L

T

K

A

L

L

K

T

S

E

V

L

D

G

L

L

E

G

D

T

R

K

I

T

D

Q

Q

N

R

R

P

P

T

S

K

Q

L

L

S

S

G

G

E

Q

R

Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

A

A

L

L

V

M

N

N

N

G

P

G

S

D

I

V

I

I

L

L

A

A

D

D

E

E

P

P

T

T

G

G

A

A

L

L

D

D

S

S

K

H

T

S

G

G

D

V

I

E

I

V

L

M

D

R

L

I

L

L

K

R

D

E

L

L

H

N

K

A

E

G

N

H

V

-

T

T

L

I

V

I

M

L

V

V

T

T

H

H

E

D

P

M

Y

Q

V

V

A

A

N

K

M

N

A

A

D

T

R

R

I

I

T

E

V

I

R

S

D

D

G

G

K

E

I

I

L

I

E

S

E

D

K

R

T

P

N

N

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: S43 (= S43), G44 (= G44), S48 (= S48)

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
45% identity, 95% coverage: 6:223/229 of query aligns to 2:217/241 of 4u00A

query
sites
4u00A

I

I

E

R

I

I

K

R

G

N

L

L

K

H

K

K

S

W

Y
x
F

D

-

K

-

G

G

K

P

I

L

K

H

A
x
V

L

L

N

K

G

G

I

I

D

H

L

L

E

E

I

V

R

A

K

P

G

G

E

E

F

K

V

L

S

V

I

I

G

G

P

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

N

R

M

T

I

I

G

N

A

R

L

L

D

E

T

D

A

F

G

Q

E

E

G

G

S

E

V

V

E

V

V

V

A

D

G

G

I

L

D

S

L

V

T

K

K

D

T

D

K

R

D

A

L

L

S

R

K

E

F

I

R

R

S

-

Q

R

E

E

I

V

G

G

F

M

V

V

F

F

Q

Q

L

Q

H

F

N

N

L

L

I

F

P

P

N

H

L

M

T

T

V

V

L

L

E

E

N

N

V

V

Q

T

I

L

-

A

P

P

M

M

I

R

G

V

T

R

S

R

I

W

S

P

G

R

K

E

D

K

M

A

E

E

K

K

R

K

A

A

L

L

K

E

L

L

K

E

S

R

V

V

D

G

L

I

E

L

D

D

R

Q

I

A

D

R

Q

K

R

Y

P

P

T

A

K

Q

L

L

S

S

G

G

E

Q

R

Q

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

V

A

N

M

N

E

P

P

S

K

I

I

I

M

L

L

A

F

D

D

E

E

P

P

T

T

G

S

A

A

L

L

D

D

S

P

K

E

T

M

G

V

D

G

I

E

I

V

L

L

D

D

L

V

L

M

K

R

D

D

L

L

H

-

K

A

K

Q

E

G

N

G

V

M

T

T

L

M

V

V

M

V

V

V

T

T

H

H

E

E

-

M

P

G

Y

F

V

A

A

R

N

E

M

V

A

A

D

D

R

R

I

V

T

F

V

M

R

D

D

G

G

G

K

Q

I

I

L

V

E

E

binding adenosine-5'-diphosphate: F12 (≠ Y16), V17 (≠ A23), S37 (= S43), G38 (= G44), S39 (= S45), G40 (= G46), K41 (= K47), S42 (= S48), T43 (= T49)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
45% identity, 90% coverage: 19:225/229 of query aligns to 12:219/240 of 4ymuJ

query
sites
4ymuJ

G

G

K

S

I

L

K

E

A
x
V

L

L

N

K

G

G

I

V

D

T

L

L

E

K

I

V

R

N

K

K

G

G

E

E

F

V

V

V

S

V

I

I

G

G

P

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

N

R

M

C

I

I

G

N

A

L

L

L

D

E

T

E

A

P

G

T

E

K

G

G

S

E

V

V

E

F

V

I

A

D

G

G

I

V

D

K

L

I

T

N

K

N

T

G

K

K

-

V

D

N

L

I

S

N

K

K

F

V

R

R

S

-

Q

Q

E

K

I

V

G

G

F

M

V

V

F

F

Q

Q

L

H

H

F

N

N

L

L

I

F

P

P

N

H

L

L

T

T

V

A

L

I

E

E

N

N

V

I

Q

T

I

L

-

A

P

P

M

V

I

K

G

V

T

K

S

K

I

M

S

N

G

K

K

K

D

E

M

A

E

E

K

E

R

L

A

A

L

V

K

D

L

L

K

A

S

K

V

V

D

G

L

L

E

L

D

D

R

K

I

K

D

D

Q

Q

R

Y

P

P

T

I

K

K

L

L

S

S

G

G

E

Q

R

K

Q

Q

R

R

V

L

A

A

I

I

A

A

R

R

A

A

L

L

V

A

N

M

N

Q

P

P

S

E

I

V

I

M

L

L

A

F

D

D

E
|
E

P

P

T

T

G

S

A

A

L

L

D

D

S

P

K

E

T

M

G

V

D

K

I

E

I

V

L

L

D

N

L

V

L

M

K

K

D

Q

L

L

H

-

K

A

K

N

E

E

N

G

V

M

T

T

L

M

V

V

M

V

V

V

T

T

H
|
H

E

E

P

M

-

G

Y

F

V

A

A

R

N

E

M

V

A

G

D

D

R

R

I

V

I

I

T

F

V

M

R

D

D

D

G

G

K

V

I

I

L

V

E

E

K

G

T

T

binding adenosine-5'-triphosphate: V16 (≠ A23), S36 (= S43), G37 (= G44), S38 (= S45), G39 (= G46), K40 (= K47), S41 (= S48), T42 (= T49), E162 (= E168), H194 (= H201)
binding magnesium ion: S41 (= S48), E162 (= E168)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 11

3c4jA Abc protein artp in complex with atp-gamma-s
43% identity, 96% coverage: 5:223/229 of query aligns to 2:219/242 of 3c4jA

query
sites
3c4jA

N

Q

I

M

I

I

E

D

I

V

K

H

G

Q

L

L

K

K

S

S

Y
x
F

D

-

K

-

G

G

K

S

I

L

K

E

A
x
V

L

L

N

K

G

G

I

I

D

N

L

V

E

H

I

I

R

R

K

E

G

G

E

E

F

V

V

V

S

V

I

V

I

I

G

G

P

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

N

R

M

C

I

L

G

N

A

L

L

L

D

E

T

D

A

F

G

D

E

E

G

G

S

E

V

I

E

I

V

I

A

D

G

G

I

I

D

N

L

L

-

K

T

A

K

K

T

D

K

T

D

N

L

L

S

N

K

K

F

V

R

R

S

-

Q

E

E

E

I

V

G

G

F

M

V

V

F

F

Q

Q

L

R

H

F

N

N

L

L

I

F

P

P

N

H

L

M

T

T

V

V

L

L

E

N

N

N

V

I

Q

T

I

L

-

A

P

P

M

M

I

K

G

V

T

R

S

K

I

W

S

P

G

R

K

E

D

K

M

A

E

E

K

A

R

K

A

A

L

M

K

E

L

L

K

D

S

K

V

V

D

G

L

L

E

K

D

D

R

K

I

A

D

H

Q

A

R

Y

P

P

T

D

K

S

L

L

S

S

G

G

E

Q

R

A

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

V

A

N

M

N

E

P

P

S

K

I

I

I

M

L

L

A

F

D

D

E

E

P

P

T

T

G

S

A

A

L

L

D

D

S

P

K

E

T

M

G

V

D

G

I

E

I

V

L

L

D

S

L

V

L

M

K

K

D

Q

L

L

H

-

K

A

K

N

E

E

N

G

V

M

T

T

L

M

V

V

M

V

V

V

T

T

H

H

E

E

-

M

P

G

Y

F

V

A

A

R

N

E

M

V

A

G

D

D

R

R

I

V

I

L

T

F

V

M

R

D

D

G

G

G

K

Y

I

I

L

I

E

E

binding phosphothiophosphoric acid-adenylate ester: F13 (≠ Y16), V18 (≠ A23), S38 (= S43), G39 (= G44), S40 (= S45), G41 (= G46), K42 (= K47), S43 (= S48), T44 (= T49)

3c41J Abc protein artp in complex with amp-pnp/mg2+
43% identity, 96% coverage: 5:223/229 of query aligns to 2:219/242 of 3c41J

query
sites
3c41J

N

Q

I

M

I

I

E

D

I

V

K

H

G

Q

L

L

K

K

S

S

Y
x
F

D

-

K

-

G

G

K

S

I

L

K

E

A
x
V

L

L

N

K

G

G

I

I

D

N

L

V

E

H

I

I

R

R

K

E

G

G

E

E

F

V

V

V

S

V

I

V

I

I

G

G

P

P

S
|
S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

N

R

M

C

I

L

G

N

A

L

L

L

D

E

T

D

A

F

G

D

E

E

G

G

S

E

V

I

E

I

V

I

A

D

G

G

I

I

D

N

L

L

-

K

T

A

K

K

T

D

K

T

D

N

L

L

S

N

K

K

F

V

R

R

S

-

Q

E

E

E

I

V

G

G

F

M

V

V

F

F

Q

Q

L

R

H

F

N

N

L

L

I

F

P

P

N

H

L

M

T

T

V

V

L

L

E

N

N

N

V

I

Q

T

I

L

-

A

P

P

M

M

I

K

G

V

T

R

S

K

I

W

S

P

G

R

K

E

D

K

M

A

E

E

K

A

R

K

A

A

L

M

K

E

L

L

K

D

S

K

V

V

D

G

L

L

E

K

D

D

R

K

I

A

D

H

Q

A

R

Y

P

P

T

D

K

S

L

L

S

S

G

G

E

Q

R

A

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

V

A

N

M

N

E

P

P

S

K

I

I

I

M

L

L

A

F

D
|
D

E

E

P

P

T

T

G

S

A

A

L

L

D

D

S

P

K

E

T

M

G

V

D

G

I

E

I

V

L

L

D

S

L

V

L

M

K

K

D

Q

L

L

H

-

K

A

K

N

E

E

N

G

V

M

T

T

L

M

V

V

M

V

V

V

T

T

H

H

E

E

-

M

P

G

Y

F

V

A

A

R

N

E

M

V

A

G

D

D

R

R

I

V

I

L

T

F

V

M

R

D

D

G

G

G

K

Y

I

I

L

I

E

E

binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ Y16), V18 (≠ A23), S38 (= S43), G39 (= G44), G41 (= G46), K42 (= K47), S43 (= S48), T44 (= T49)
binding magnesium ion: S43 (= S48), D163 (= D167)

2olkA Abc protein artp in complex with adp-beta-s
43% identity, 96% coverage: 5:223/229 of query aligns to 2:219/242 of 2olkA

query
sites
2olkA

N

Q

I

M

I

I

E

D

I

V

K

H

G

Q

L

L

K

K

S

S

Y
x
F

D

-

K

-

G

G

K

S

I

L

K

E

A
x
V

L

L

N

K

G

G

I

I

D

N

L

V

E

H

I

I

R

R

K

E

G

G

E

E

F

V

V

V

S

V

I

V

I

I

G

G

P

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

N

R

M

C

I

L

G

N

A

L

L

L

D

E

T

D

A

F

G

D

E

E

G

G

S

E

V

I

E

I

V

I

A

D

G

G

I

I

D

N

L

L

-

K

T

A

K

K

T

D

K

T

D

N

L

L

S

N

K

K

F

V

R

R

S

-

Q

E

E

E

I

V

G

G

F

M

V

V

F

F

Q

Q

L

R

H

F

N

N

L

L

I

F

P

P

N

H

L

M

T

T

V

V

L

L

E

N

N

N

V

I

Q

T

I

L

-

A

P

P

M

M

I

K

G

V

T

R

S

K

I

W

S

P

G

R

K

E

D

K

M

A

E

E

K

A

R

K

A

A

L

M

K

E

L

L

K

D

S

K

V

V

D

G

L

L

E

K

D

D

R

K

I

A

D

H

Q

A

R

Y

P

P

T

D

K

S

L

L

S

S

G

G

E

Q

R

A

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

V

A

N

M

N

E

P

P

S

K

I

I

I

M

L

L

A

F

D

D

E

E

P

P

T

T

G

S

A

A

L

L

D

D

S

P

K

E

T

M

G

V

D

G

I

E

I

V

L

L

D

S

L

V

L

M

K

K

D

Q

L

L

H

-

K

A

K

N

E

E

N

G

V

M

T

T

L

M

V

V

M

V

V

V

T

T

H

H

E

E

-

M

P

G

Y

F

V

A

A

R

N

E

M

V

A

G

D

D

R

R

I

V

I

L

T

F

V

M

R

D

D

G

G

G

K

Y

I

I

L

I

E

E

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (≠ Y16), V18 (≠ A23), S38 (= S43), G39 (= G44), S40 (= S45), G41 (= G46), K42 (= K47), S43 (= S48), T44 (= T49)

2oljA Abc protein artp in complex with adp/mg2+
43% identity, 96% coverage: 5:223/229 of query aligns to 2:219/242 of 2oljA

query
sites
2oljA

N

Q

I

M

I

I

E

D

I

V

K

H

G

Q

L

L

K

K

S

S

Y
x
F

D

-

K

-

G

G

K

S

I

L

K

E

A
x
V

L

L

N

K

G

G

I

I

D

N

L

V

E

H

I

I

R

R

K

E

G

G

E

E

F

V

V

V

S

V

I

V

I

I

G

G

P

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

N

R

M

C

I

L

G

N

A

L

L

L

D

E

T

D

A

F

G

D

E

E

G

G

S

E

V

I

E

I

V

I

A

D

G

G

I

I

D

N

L

L

-

K

T

A

K

K

T

D

K

T

D

N

L

L

S

N

K

K

F

V

R

R

S

-

Q

E

E

E

I

V

G

G

F

M

V

V

F

F

Q

Q

L

R

H

F

N

N

L

L

I

F

P

P

N

H

L

M

T

T

V

V

L

L

E

N

N

N

V

I

Q

T

I

L

-

A

P

P

M

M

I

K

G

V

T

R

S

K

I

W

S

P

G

R

K

E

D

K

M

A

E

E

K

A

R

K

A

A

L

M

K

E

L

L

K

D

S

K

V

V

D

G

L

L

E

K

D

D

R

K

I

A

D

H

Q

A

R

Y

P

P

T

D

K

S

L

L

S

S

G

G

E

Q

R

A

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

V

A

N

M

N

E

P

P

S

K

I

I

I

M

L

L

A

F

D

D

E

E

P

P

T

T

G

S

A

A

L

L

D

D

S

P

K

E

T

M

G

V

D

G

I

E

I

V

L

L

D

S

L

V

L

M

K

K

D

Q

L

L

H

-

K

A

K

N

E

E

N

G

V

M

T

T

L

M

V

V

M

V

V

V

T

T

H

H

E

E

-

M

P

G

Y

F

V

A

A

R

N

E

M

V

A

G

D

D

R

R

I

V

I

L

T

F

V

M

R

D

D

G

G

G

K

Y

I

I

L

I

E

E

binding adenosine-5'-diphosphate: F13 (≠ Y16), V18 (≠ A23), S38 (= S43), G39 (= G44), S40 (= S45), G41 (= G46), K42 (= K47), S43 (= S48), T44 (=