SitesBLAST

Q6Z2T3 Aquaporin NIP2-1; Low silicon protein 1; NOD26-like intrinsic protein 2-1; OsNIP2;1; Silicon influx transporter LSI1 from Oryza sativa subsp. japonica (Rice) (see paper)
38% identity, 95% coverage: 4:247/258 of query aligns to 47:261/298 of Q6Z2T3

query
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Q6Z2T3

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A132 (= A106) mutation to T: In lsi; impairs silicon uptake. Grain discoloration. Reduces grain yield 10-fold.

P08995 Nodulin-26; N-26 from Glycine max (Soybean) (Glycine hispida) (see paper)
35% identity, 98% coverage: 6:257/258 of query aligns to 38:260/271 of P08995

query
sites
P08995

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Sites not aligning to the query:

262 modified: Phosphoserine; by CPK

P47863 Aquaporin-4; AQP-4; Mercurial-insensitive water channel; MIWC; WCH4 from Rattus norvegicus (Rat) (see 4 papers)
43% identity, 74% coverage: 54:243/258 of query aligns to 69:251/323 of P47863

query
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P47863

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S180 (≠ D167) modified: Phosphoserine; by PKC; mutation to A: Decreases internalization from the cell membrane in response to PKC activation.
H201 (≠ I187) mutation to P: Partial loss of transport activity.

Sites not aligning to the query:

13 modified: S-palmitoyl cysteine; C→A: Reduced palmitoylation. Loss of palmitoylation; when associated with A-17.
17 modified: S-palmitoyl cysteine; C→A: Reduced palmitoylation. Loss of palmitoylation; when associated with A-13.
285 modified: Phosphoserine

P55088 Aquaporin-4; AQP-4; Mercurial-insensitive water channel; MIWC; WCH4 from Mus musculus (Mouse) (see 2 papers)
43% identity, 74% coverage: 54:243/258 of query aligns to 69:251/323 of P55088

query
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P55088

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S111 (≠ P96) modified: Phosphoserine; by PKG; mutation to A: Loss of phosphorylation by PKG (in vitro). No effect on location at cell membrane.

Sites not aligning to the query:

4 natural variant: G -> R

B1VB61 Propanediol uptake facilitator PduF from Citrobacter freundii (see paper)
32% identity, 99% coverage: 3:258/258 of query aligns to 4:266/269 of B1VB61

query
sites
B1VB61

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NPA 66:68 (= NPA 82:84) NPA 1
NPA 201:203 (= NPA 199:201) NPA 2

Q9SAI4 Aquaporin NIP6-1; NOD26-like intrinsic protein 6-1; AtNIP6;1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
35% identity, 98% coverage: 2:253/258 of query aligns to 76:300/305 of Q9SAI4

query
sites
Q9SAI4

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I

L

V

I

I

Y

L

A

S

M

T

G

G

H

H

I

I

S

S

G

G

C

A

H

H

I

L

N

N

P

P

A

A

V

V

T

T

I

I

A

A

L

F

W

A

A

A

T

L

K

K

K

H

F

F

P

P

G

W

N

K

E

H

V

V

V

P

P

V

Y

Y

I

I

I

G

A

A

Q

Q

L

V

I

M

G

A

A

S

S

V

L

S

A

A

S

A

F

F

L

A

F

L

A

K

A

A

S

-

V

-

G

-

M

V

N

F

A

E

V

P

T

T

V

M

G

S

G

G

L

-

G

G

A

V

T

T

A

V

P

P

F

T

E

V

G

G

I

L

S

S

Y

-

Q

Q

A

A

I

F

L

A

V

L

E

E

T

F

I

I

G

I

T

S

F

F

L

N

L

L

M

M

F

F

V

V

I

V

M

T

G

A

S

V

G

A

V

T

D

D

R

T

R

R

A

A

V

V

P

G

G

E

F

L

A

A

G

G

I

I

S

A

I

V

G

G

F

A

T

T

V

V

A

M

A

L

V

N

I

I

T

L

T

I

I

A

G

G

N

P

I

A

S

T

G

S

A

A

S

S

L

M

N

N

P

P

A
x
V

R

R

T

T

F

L

G

G

P

P

Y

-

L

-

G

-

D

-

Y

-

V

A

L

I

A

A

G

A

N

N

-

Y

-

R

-

A

L

I

W

W

N

-

Y

-

F

-

P

-

I

V

Y

Y

I

L

I

T

G

A

P

P

I

I

V

L

G

G

A

A

V

L

L

I

A

G

A

A

F

G

I

T

Y

Y

E

T

Y

I

I

V

S

K

-

L

-

P

S

E

E

E

E

D

K

E

L

A

P

P

A

K

Q

E

R

R

A119 (≠ F62) mutation to W: 6-fold increase in water transport activity, but impaired in urea transport.
V252 (≠ A201) mutation to A: No effect.

P37451 Propanediol uptake facilitator PduF from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
32% identity, 98% coverage: 3:254/258 of query aligns to 4:262/264 of P37451

query
sites
P37451

S

S

L

L

K

K

K

A

R

Q

F

C

L

G

A

A

E

E

L

F

I

L

G

G

T

T

F

G

F

L

L

F

V

L

F

F

M

F

G

G

A

I

G

G

A

C

A

L

A

S

I

-

T

A

L

L

M

K

I

V

S

A

K

G

G

A

S

S

A

-

V

-

P

-

N

-

A

-

F

-

N

-

I

-

G

-

I

-

G

-

A

-

L

-

G

-

L

L

G

G

D

L

W

W

L

-

A

E

V

I

G

C

L

I

A

I

F

W

G

G

L

L

T

G

I

I

V

S

V

L

L

A

I

V

Y

Y

A

L

M

T

G

A

H

G

I

I

S

S

G

G

C

G

H

H

I

L

N
|
N

P
|
P

A
|
A

V

V

T

T

I

I

A

A

L

L

W

W

A

L

T

F

K

A

K

C

F

F

P

P

G

K

N

Q

E

K

V

V

V

L

P

P

Y

Y

I

I

A

A

Q

Q

L

F

I

A

G

G

A

A

-

F

-

G

S

A

L

L

A

L

S

A

F

Y

L

V

F

L

A

Y

A

S

S

S

V

L

-

F

-

T

-

E

-

F

-

E

-

T

-

A

-

H

-

M

-

V

-

R

-

G

G

S

M

V

N

E

A

S

V

L

T

Q

V

L

G

A

G

S

L

I

G

F

A

S

T

T

A

Y

P

P

F

A

E

A

G

A

I

L

S

N

Y

V

Y

W

Q

Q

A

A

I

A

L

L

V

V

E

E

T

V

I

V

G

I

T

T

F

S

L

I

L

L

M

M

F

G

V

M

I

I

M

M

G

A

S

L

G

T

V

D

D

D

R

G

R

N

A

G

V

I

P

P

G

K

-

G

-

P

F

L

A

A

G

P

I

L

S

L

I

I

G

G

F

I

T

L

V

V

A

A

A

V

V

I

I

G

T

A

T

S

I

T

G

G

N

P

I

L

S

T

G

G

A

F

S

A

L

M

N
|
N

P
|
P

A
|
A

R

R

T

D

F

F

G

G

P

P

Y

K

L

L

-

F

-

T

-

W

-

L

-

A

-

G

-

W

G

G

D

N

Y

M

V

A

L

M

A

S

G

G

N

G

N

R

L

E

W

I

N

P

Y

Y

F

F

P

I

I

V

Y

P

I

I

I

V

G

A

P

P

I

V

V

I

G

G

A

A

V

C

L

A

A

G

A

A

F

A

I

I

Y

Y

E

R

Y

Y

I

F

S

I

S

G

E

-

E

K

K

N

L

L

P

P

A

C

Q

N

R

R

V

C

NPA 66:68 (= NPA 82:84) NPA 1
NPA 201:203 (= NPA 199:201) NPA 2

P0AER0 Glycerol uptake facilitator protein; Aquaglyceroporin; Glycerol facilitator from Escherichia coli (strain K12) (see 3 papers)
31% identity, 99% coverage: 3:258/258 of query aligns to 6:268/281 of P0AER0

query
sites
P0AER0

S

T

L

L

K

K

K

G

R

Q

F

C

L

I

A

A

E

E

L

F

I

L

G

G

T

T

F

G

F

L

L

L

V

I

F

F

M

F

G

G

A

V

G

G

A

C

A

V

A

A

I

-

T

A

L

L

M

K

I

V

S

A

K

G

G

A

S

S

A

-

V

-

P

-

N

-

A

-

F

-

N

-

I

-

G

-

I

-

G

-

A

-

L

-

G

-

L

F

G

G

D

Q

W

W

L

-

A

E

V

I

G

S

L

V

A

I

F

W

G

G

L

L

T

G

I

V

V

A

V

M

L

A

I

I

Y

Y

A

L

M

T

G

A

H

G

I

V

S

S

G

G

C

A

H
|
H

I
x
L

N
|
N

P

P

A

A

V

V

T

T

I

I

A

A

L

L

W

W

A

L

T

F

K

A

K

C

F

F

P

D

G

K

N

R

E

K

V

V

V

I

P

P

Y

F

I

I

I

V

A

S

Q

Q

L

V

I

A

G

G

A

A

S

F

L

C

A

A

S

A

-

A

-

L

-

V

-

Y

-

G

-

L

-

Y

-

N

-

L

-

F

F

F

L

D

F

F

A

E

A

Q

S

T

V

H

-

H

-

I

-

V

-

R

-

G

G

S

M

V

N

E

A

S

V

V

T

D

V

L

G

A

G

G

L

T

G

F

A

S

T

T

A
x
Y

P

P

F

N

E

P

G

H

I

I

S

N

Y

F

Y

V

Q

Q

A

A

I

F

L

A

V

V

E

E

T

M

I

V

G

I

T

T

F

A

L

I

L

L

M

M

F

G

V

L

I

I

M

L

G

A

S

L

G

T

V

D

D

D

R

G

R

N

A

G

V

V

P

P

G

R

-

G

-

P

F

L

A

A

G

P

I

L

S

L

I

I

G

G

F

L

T

L

V

I

A

A

A

V

V

I

I

G

T

A

T

S

I

M

G

G

N

P

I

L

S

T

G
|
G

A
x
F

S
x
A

L

M

N
|
N

P

P

A

A

R
|
R

T

D

F

F

G

G

P

P

Y

K

L

V

-

F

-

A

-

W

-

L

-

A

-

G

-

W

G

G

D

N

Y

V

V

A

L

F

A

T

G

G

N

G

N

R

L

D

W

I

N

P

Y

Y

F

F

P

L

I

V

Y
x
P

I
x
L

I

F

G

G

P

P

I

I

V

V

G

G

A

A

V

I

L

V

A

G

A

A

F

F

I

A

Y

Y

E

R

Y

K

I

L

S

I

S

G

E

R

E

H

K

-

L

L

P

P

A

C

Q

D

R

I

V

C

T

V

R

V

E

E

K

E

HLN 66:68 (≠ HIN 80:82) binding glycerol
Y138 (≠ A136) binding glycerol
GFA 199:201 (≠ GAS 195:197) binding glycerol
N203 (= N199) binding glycerol
R206 (= R202) binding glycerol
PL 236:237 (≠ YI 225:226) mutation to FW: No detectable water or glycerol permeability.

P60844 Aquaporin Z; Bacterial nodulin-like intrinsic protein; Water channel AqpZ from Escherichia coli (strain K12) (see paper)
38% identity, 77% coverage: 6:203/258 of query aligns to 3:190/231 of P60844

query
sites
P60844

K

R

R

K

F

L

L

A

A

A

E

E

L
x
C

I

F

G

G

T

T

F

F

F

W

L

L

V

V

F

F

M

G

G

G

A
x
C

G

G

A

S

A

A

A

V

I

L

T

A

L

-

M

-

I

-

S

-

K

-

G

-

S

A

A

G

V

F

P

P

N

E

A

-

F

-

N

-

I

L

G

G

I

I

G

G

A

F

L

A

G

G

G

-

L

-

G

-

D

-

W

-

L

-

A

-

V

V

G

A

L

L

A

A

F

F

G

G

L

L

T

T

I

V

V

L

V

T

L

M

I

A

Y

F

A

A

M

V

G

G

H

H

I

I

S

S

G

G

C

G

H

H

I

F

N

N

P

P

A

A

V

V

T

T

I

I

A

G

L

L

W

W

A

A

T

G

K

G

K

R

F

F

P

P

G

A

N

K

E

E

V

V

P

G

Y

Y

I

V

I

I

A

A

Q

Q

L

V

I

V

G

G

-

G

-

I

-

V

-

A

A

A

S

A

L

L

A

L

S

Y

F

L

L

I

F

A

A

S

A

G

S

K

V

T

G

G

M

F

N

D

A

A

V

A

T

A

V

S

G

G

-

F

-

A

-

S

-

N

G

G

L

Y

G

G

A

E

T

H

A

S

P

P

F

-

E

G

G

G

I

Y

S

S

Y

M

Y

L

Q

S

A

A

I

L

L

V

V

V

E

E

T

L

I

V

G

L

T

S

F

A

L

G

L

F

M

L

F

L

V

V

I

I

M

H

G

G

S

A

G

-

V

T

D

D

R

K

R

F

A

A

V

P

P

A

G

G

F

F

A

A

G

P

I

I

S

A

I

I

G

G

F

L

T

A

V

L

A

T

A

L

V

I

I

H

T

L

T

I

I

S

G

I

N

P

I

V

S

T

G

N

A
x
T

S

S

L

V

N

N

P

P

A

A

R
|
R

T

S

C9 (≠ L12) mutation to S: No effect.
C20 (≠ A23) mutation to S: Loss of oligomerization; no alteration of water permeability.
T183 (≠ A196) mutation to C: No effect.
R189 (= R202) mutation R->V,S: Loss of function.

1fx8A Crystal structure of the e. Coli glycerol facilitator (glpf) with substrate glycerol (see paper)
31% identity, 93% coverage: 3:241/258 of query aligns to 1:247/254 of 1fx8A

query
sites
1fx8A

S

T

L

L

K

K

K

G

R

Q

F

C

L

I

A

A

E

E

L

F

I

L

G

G

T

T

F

G

F

L

L

L

V

I

F

F

M

F

G

G

A

V

G

G

A

C

A

V

A

A

I

-

T

A

L

L

M

K

I

V

S

A

K

G

G

A

S

S

A

-

V

-

P

-

N

-

A

-

F

-

N

-

I

-

G

-

I

-

G

-

A

-

L

-

G

-

L

F

G

G

D

Q

W

W

L

-

A

E

V

I

G

S

L

V

A

I

F
x
W

G

G

L

L

T

G

I

V

V

A

V

M

L

A

I

I

Y

Y

A

L

M

T

G

A

H

G

I

V

S

S

G

G

C

A

H

H

I

L

N
|
N

P

P

A

A

V

V

T

T

I

I

A

A

L

L

W

W

A

L

T

F

K

A

K

C

F

F

P

D

G

K

N

R

E

K

V

V

V

I

P

P

Y

F

I

I

I

V

A

S

Q

Q

L

V

I

A

G

G

A

A

S

F

L

C

A

A

S

A

-

A

-

L

-

V

-

Y

-

G

-

L

-

Y

-

N

-

L

-

F

F

F

L

D

F

F

A

E

A

Q

S

T

V

H

-

H

-

I

-

V

-

R

-

G

G

S

M

V

N

E

A

S

V

V

T

D

V

L

G

A

G

G

L

T

G

F

A

S

T

T

A

Y

P

P

F

N

E

P

G

H

I

I

S

N

Y

F

Y

V

Q

Q

A

A

I

F

L

A

V

V

E

E

T

M

I

V

G

I

T

T

F

A

L

I

L

L

M

M

F

G

V

L

I

I

M

L

G

A

S

L

G

T

V

D

D

D

R

G

R

N

A

G

V

V

P

P

G

R

-

G

-

P

F

L

A

A

G

P

I

L

S

L

I

I

G

G

F

L

T

L

V

I

A

A

A

V

V

I

I

G

T

A

T

S

I

M

G

G

N

P

I

L

S

T

G
|
G

A
x
F

S
x
A

L

M

N
|
N

P

P

A

A

R
|
R

T

D

F

F

G

G

P

P

Y

K

L

V

-

F

-

A

-

W

-

L

-

A

-

G

-

W

G

G

D

N

Y

V

V

A

L

F

A

T

G

G

N

G

N

R

L

D

W

I

N

P

Y

Y

F

F

P

L

I

V

Y

P

I

L

I

F

G

G

P

P

I

I

V

V

G

G

A

A

V

I

L

V

A

G

A

A

F

F

I

A

Y

Y

E

R

binding glycerol: W43 (≠ F62), N63 (= N82), G194 (= G195), F195 (≠ A196), A196 (≠ S197), N198 (= N199), R201 (= R202)

8uy6A Aquaporin z with alfa tag and bound to nanobody (see paper)
38% identity, 77% coverage: 6:203/258 of query aligns to 3:190/244 of 8uy6A

query
sites
8uy6A

K

R

R

K

F

L

L

A

A

A

E

E

L
x
C

I
x
F

G

G

T

T

F
|
F

F

W

L

L

V

V

F

F

M

G

G

G

A

C

G

G

A

S

A

A

A

V

I

L

T

A

L

-

M

-

I

-

S

-

K

-

G

-

S

A

A

G

V

F

P

P

N

E

A

-

F

-

N

-

I

L

G

G

I

I

G

G

A

F

L

A

G

G

G

-

L

-

G

-

D

-

W

-

L

-

A

-

V

V

G

A

L

L

A

A

F

F

G

G

L

L

T

T

I

V

V

L

V

T

L

M

I

A

Y

F

A

A

M

V

G

G

H

H

I

I

S

S

G

G

C

G

H

H

I

F

N

N

P

P

A

A

V

V

T

T

I

I

A

G

L

L

W

W

A

A

T

G

K

G

K

R

F

F

P

P

G

A

N

K

E

E

V

V

P

G

Y

Y

I

V

I

I

A

A

Q

Q

L

V

I

V

G

G

-

G

-
x
I

-
x
V

-

A

A

A

S

A

L

L

A

L

S
x
Y

F

L

L

I

F

A

A

S

A

G

S

K

V

T

G

G

M

F

N

D

A

A

V

A

T

A

V

S

G

G

-

F

-

A

-

S

-

N

G

G

L

Y

G

G

A

E

T

H

A

S

P

P

F

-

E

G

G

G

I

Y

S

S

Y

M

Y

L

Q

S

A

A

I

L

L

V

V

V

E

E

T

L

I

V

G

L

T

S

F

A

L

G

L

F

M

L

F

L

V

V

I

I

M

H

G

G

S

A

G

-

V

T

D

D

R

K

R

F

A

A

V

P

P

A

G

G

F

F

A

A

G

P

I

I

S

A

I

I

G

G

F

L

T

A

V

L

A

T

A

L

V

I

I

H

T

L

T

I

I

S

G

I

N

P

I

V

S

T

G

N

A

T

S

S

L

V

N

N

P

P

A

A

R

R

T

S

binding cardiolipin: C9 (≠ L12), F10 (≠ I13), F13 (= F16), I93 (vs. gap), V94 (vs. gap), Y100 (≠ S115)

Sites not aligning to the query:

binding cardiolipin: 195, 196, 209

2o9eA Crystal structure of aqpz mutant t183c complexed with mercury (see paper)
38% identity, 77% coverage: 6:203/258 of query aligns to 5:192/232 of 2o9eA

query
sites
2o9eA

K

R

R

K

F

L

L

A

A

A

E

E

L

S

I

F

G

G

T

T

F

F

F

W

L

L

V

V

F

F

M

G

G

G

A

S

G

G

A

S

A

A

A

V

I

L

T

A

L

-

M

-

I

-

S

-

K

-

G

-

S

A

A

G

V

F

P

P

N

E

A

-

F

-

N

-

I

L

G

G

I

I

G

G

A

F

L

A

G

G

G

-

L

-

G

-

D

-

W

-

L

-

A

-

V

V

G

A

L

L

A

A

F

F

G

G

L

L

T

T

I

V

V

L

V

T

L

M

I

A

Y

F

A

A

M

V

G

G

H

H

I

I

S

S

G

G

C

G

H

H

I

F

N

N

P

P

A

A

V

V

T

T

I

I

A

G

L

L

W

W

A

A

T

G

K

G

K

R

F

F

P

P

G

A

N

K

E

E

V

V

P

G

Y

Y

I

V

I

I

A

A

Q

Q

L

V

I

V

G

G

-

G

-

I

-

V

-

A

A

A

S

A

L

L

A

L

S

Y

F

L

L

I

F

A

A

S

A

G

S

K

V

T

G

G

M

F

N

D

A

A

V

A

T

A

V

S

G

G

-

F

-

A

-

S

-

N

G

G

L

Y

G

G

A

E

T

H

A

S

P

P

F

-

E

G

G

G

I

Y

S

S

Y

M

Y

L

Q

S

A

A

I

L

L

V

V

V

E
|
E

T

L

I

V

G

L

T

S

F

A

L

G

L

F

M

L

F

L

V

V

I

I

M

H

G

G

S

A

G

-

V

T

D

D

R

K

R

F

A

A

V

P

P

A

G

G

F

F

A

A

G

P

I

I

S

A

I

I

G

G

F

L

T

A

V

L

A

T

A

L

V

I

I

H

T

L

T

I

I
x
S

G

I

N

P

I

V

S

T

G

N

A

C

S

S

L

V

N

N

P

P

A

A

R

R

T

S

binding mercury (ii) ion: E140 (= E150), S179 (≠ I190)

3nkaA Crystal structure of aqpz h174g,t183f (see paper)
40% identity, 77% coverage: 6:203/258 of query aligns to 5:192/230 of 3nkaA

query
sites
3nkaA

K

R

R

K

F

L

L

A

A

A

E

E

L

C

I

F

G

G

T

T

F

F

F

W

L

L

V

V

F

F

M

G

G

G

A

C

G

G

A

S

A
|
A

A

V

I

L

T

A

L

-

M

-

I

-

S

-

K

-

G

-

S

A

A

G

V

F

P

P

N

E

A

-

F

-

N

-

I

L

G

G

I

I

G

G

A

F

L

A

G

G

G

-

L

-

G

-

D

-

W

-

L

-

A

-

V
|
V

G

A

L

L

A

A

F

F

G

G

L

L

T

T

I

V

V

L

V

T

L

M

I

A

Y

F

A

A

M

V

G

G

H

H

I

I

S

S

G

G

C

G

H

H

I

F

N

N

P

P

A

A

V

V

T

T

I

I

A

G

L

L

W

W

A

A

T

G

K

G

K

R

F

F

P

P

G

A

N

K

E

E

V

V

P

G

Y

Y

I

V

I

I

A

A

Q

Q

L

V

I

V

G

G

-

G

-

I

-

V

-

A

A

A

S

A

L

L

A

L

S

Y

F

L

L

I

F

A

A

S

A

G

S

K

V

T

G

G

M

F

N

D

A

A

V

A

T

A

V

S

G

G

-

F

-

A

-

S

-

N

G

G

L

Y

G

G

A

E

T

H

A

S

P

P

F

-

E

G

G

G

I

Y

S

S

Y

M

Y

L

Q

S

A

A

I

L

L

V

V

V

E

E

T

L

I

V

G

L

T

S

F

A

L

G

L

F

M

L

F

L

V

V

I

I

M

H

G

G

S

A

G

-

V

T

D

D

R

K

R

F

A

A

V

P

P

A

G

G

F

F

A

A

G

P

I

I

S

A

I

I

G

G

F

L

T

A

V

-

A

-

V

-

I

L

T

T

T

L

I

I

G

G

N

L

I

I

S

S

-
x
I

-

P

-

V

-

T

G
x
N

A

F

S

S

L

V

N

N

P

P

A

A

R

R

T

S

binding glycerol: A25 (= A26), V41 (= V58), I180 (vs. gap), N184 (≠ G195)

P29972 Aquaporin-1; AQP-1; Aquaporin-CHIP; Channel-like integral membrane protein of 28 kDa; Urine water channel from Homo sapiens (Human) (see 4 papers)
32% identity, 93% coverage: 5:243/258 of query aligns to 7:230/269 of P29972

query
sites
P29972

K

K

R

L

F

F

L

W

A

R

E

A

L

V

I

V

G

A

T

E

F

F

F

L

L

-

V

-

F

-

M

-

G

-

A

-

G

-

A

A

A

T

I

L

T

F

L

V

M

F

I

I

S

S

K

I

G

G

S

S

A

A

V

L

P

G

N

F

A

K

F

Y

N
x
P

I

V

G

G

I

N

G

N

A

Q

L

T

G
x
A

G

-

L

V

G

Q

D

D

W

N

L

V

A

K

V

V

G

S

L

L

A

A

F

F

G

G

L

L

T

S

I

I

V

A

V

T

L

L

I

A

Y

Q

A

S

M

V

G

G

H

H

I

I

S

S

G

G

C

A

H

H

I

L

N

N

P

P

A

A

V

V

T

T

I

L

A

G

L

L

W

L

A

L

T

S

K

C

K

Q

F

I

P

S

G

I

N

F

E

R

V

A

V

L

P

M

Y

Y

I

I

A

A

Q

Q

L

C

I

V

G

G

A

A

S

I

L

V

A

A

S

T

F

A

L

I

F

L

A

S

A

G

S

I

V

T

G

-

M

-

N

S

A

S

V

L

T

T

V

G

G

N

G

S

L

L

G

G

A

R

T

N

A

D

P

L

F

A

E

D

G

G

I

V

S

N

Y

S

Y

G

Q

Q

A

G

I

L

L

G

V

I

E

E

T

I

I

I

G

G

T

T

F

L

L

Q

L

L

M

V

F

L

V

C

I

V

M

L

G

A

-

T

S

T

G

D

V

R

D

R

R

R

A

D

V

L

P
x
G

G

G

F

S

A

A

G

P

I

L

S

A

I

I

G

G

F

L

T

S

V

V

A

A

A

L

V

G

I
x
H

T

L

I

A

G

I

N

D

I

Y

S

T

G

G

A
x
C

S

G

L

I

N

N

P

P

A

A

R

R

T

S

F

F

G

G

P

S

Y

-

L

-

G

-

D

-

Y

-

V

A

L

V

A

I

G

T

N

H

N

N

L

F

W

S

N

N

Y
x
H

F

W

P

-

I

I

Y

F

I

W

I

V

G

G

P

P

I

F

V

I

G

G

A

G

V

A

L

L

A

A

A

V

F

L

I

I

Y

Y

E

D

Y

F

I

I

P38 (≠ N43) to L: in Co(A-B-) antigen; non functional AQP1; red cells show low osmotic water permeability; dbSNP:rs104894004
A45 (≠ G50) to V: in Co(A-B+) antigen; dbSNP:rs28362692
G165 (≠ P172) to D: in dbSNP:rs28362731
H180 (≠ I187) mutation to A: No effect on water channel activity.
C189 (≠ A196) Hg(2+)-sensitive residue; mutation to S: No effect on water channel activity.
H209 (≠ Y221) mutation to A: Decreased water channel activity.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

8ct2D Local refinement of aqp1 tetramer (c1; refinement mask included d1 of protein 4.2 and ankyrin-1 ar1-5) in class 2 of erythrocyte ankyrin-1 complex (see paper)
32% identity, 93% coverage: 5:243/258 of query aligns to 5:228/247 of 8ct2D

query
sites
8ct2D

K

K

R

L

F

F

L

W

A

R

E

A

L

V

I

V

G

A

T

E

F

F

F

L

L

-

V

-

F

-

M

-

G

-

A

-

G

-

A

A

A

T

I

L

T

F

L

V

M

F

I

I

S

S

K

I

G

G

S

S

A

A

V

L

P

G

N

F

A

K

F

Y

N

P

I

V

G

G

I

N

G

N

A

Q

L

T

G

A

G

-

L

V

G

Q

D

D

W

N

L

V

A

K

V

V

G

S

L

L

A

A

F

F

G

G

L

L

T

S

I

I

V

A

V

T

L

L

I

A

Y

Q

A

S

M

V

G

G

H

H

I

I

S

S

G

G

C

A

H

H

I

L

N

N

P

P

A

A

V

V

T

T

I

L

A

G

L

L

W

L

A

L

T

S

K

C

K

Q

F

I

P

S

G

I

N

F

E

R

V

A

V

L

P

M

Y

Y

I

I

A

A

Q

Q

L

C

I

V

G

G

A

A

S

I

L

V

A

A

S

T

F

A

L

I

F

L

A

S

A

G

S

I

V

T

G

-

M

-

N

S

A

S

V

L

T

T

V

G

G

N

G

S

L

L

G

G

A

R

T

N

A

D

P

L

F

A

E

D

G

G

I

V

S

N

Y

S

Y
x
G

Q

Q

A

G

I

L

L
x
G

V

I

E

E

T

I

I

I

G

G

T

T

F

L

L

Q

L

L

M

V

F

L

V

C

I

V

M

L

G

A

-

T

S

T

G

D

V

R

D

R

R

R

A

D

V

L

P

G

G

G

F

S

A

A

G

P

I

L

S

A

I

I

G

G

F

L

T

S

V

V

A

A

A

L

V

G

I

H

T

L

I

A

G

I

N

D

I

Y

S

T

G

G

A

C

S

G

L

I

N

N

P

P

A

A

R

R

T

S

F

F

G

G

P

S

Y

-

L

-

G

-

D

-

Y

-

V

A

L

V

A

I

G

T

N

H

N

N

L

F

W

S

N

N

Y

H

F

W

P

-

I

I

Y

F

I

W

I

V

G

G

P

P

I

F

V

I

G

G

A

G

V

A

L

L

A

A

A

V

F

L

I

I

Y

Y

E

D

Y

F

I

I

binding cholesterol: G134 (≠ Y144), G138 (≠ L148)

P43286 Aquaporin PIP2-1; Plasma membrane intrinsic protein 2-1; AtPIP2;1; Plasma membrane intrinsic protein 2a; PIP2a from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
31% identity, 93% coverage: 3:243/258 of query aligns to 36:269/287 of P43286

query
sites
P43286

S

S

L

F

K

Y

K

R

R

A

F

V

L

I

A

A

E

E

L

F

I

V

G

A

T

T

F

L

L

F

V

L

F

Y

M

-

G

-

A

-

G

-

A

-

I

I

T

T

L

V

M

L

I

T

S

V

K

I

G

G

S

Y

A

K

V

I

P

Q

N

S

A

D

F

T

N

D

I

A

G

G

I

G

G

V

A

D

L

C

G

G

L

V

G

G

D

I

W

-

L

L

A

G

V

I

G

A

L

W

A

A

F

F

G

G

L

G

T

M

I

I

V

F

V

I

L

L

I

V

Y

Y

A

C

M

T

G

A

H

G

I

I

S

S

G

G

C

G

H

H

I

I

N

N

P

P

A

A

V

V

T

T

I

F

A

G

L

L

W

F

A

L

T

A

K

R

K

K

F

V

P

S

G

L

N

P

E

R

V

A

V

L

P

L

Y

Y

I

I

A

A

Q

Q

L

C

I

L

G

G

A

A

S

-

L

I

A

C

S

G

F

V

L

G

F

F

A

V

A

K

S

A

V

F

G

Q

M

S

N

S

A

Y

V

Y

T

T

V

R

G

Y

G

G

L

G

G

G

A

A

T

N

A

S

P

L

F

A

E

D

G

G

I

Y

S

S

Y

T

Y

G

Q

T

A

G

I

L

L

A

V

A

E

E

T

I

I

I

G

G

T

T

F

F

L

V

L

L

M

V

F

Y

V

T

I

V

M

F

G

S

S

A

G

T

V

D

D

P

R

K

R

R

A

S

-

A

-

R

-

D

-

S

-

H

V

V

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Sites not aligning to the query:

3 modified: N6,N6-dimethyllysine; partial; K→A: 2-fold decrease in water transport activity.; K→R: No effect.
6 E→A: No effect.
280 modified: Phosphoserine; S→A: Normal subcellular localization.
283 modified: Phosphoserine; S→A: Intracellular reticulation pattern, probably corresponding to the endoplasmic reticulum.; S→D: Normal subcellular localization.

P41181 Aquaporin-2; AQP-2; ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct from Homo sapiens (Human) (see 12 papers)
32% identity, 94% coverage: 2:243/258 of query aligns to 7:222/271 of P41181

query
sites
P41181

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G64 (= G78) to R: in NDI2; loss of water channel activity; dbSNP:rs104894326
G78 (≠ T92) mutation to A: Does not affect interaction with MIAC; when associated with A-79.
C79 (≠ K93) mutation to A: Does not affect interaction with MIAC; when associated with A-78.
S148 (= S164) mutation to A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; mutation to D: Retained in the endoplasmic reticulum.
R187 (= R202) to C: in NDI2; loss of water channel activity; mutant protein does not fold properly; dbSNP:rs104894328
A190 (≠ G205) to T: in NDI2; mutant protein does not fold properly and is not functional; dbSNP:rs104894341
V194 (vs. gap) to I: in dbSNP:rs772051028
S216 (≠ A237) to P: in NDI2; loss of water channel activity; dbSNP:rs104894329
L217 (≠ F238) mutation to A: Abolishes interaction with MIAC; when associated with A-221.
Y221 (= Y242) mutation to A: Abolishes interaction with MIAC; when associated with A-217.

Sites not aligning to the query:

229 S→A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; S→D: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.
231 S→A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; S→D: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.
232 E→A: Reduces interaction with MIAC.
244 T→A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; T→E: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.
254 R → L: in NDI2; results in the loss of arginine vasopressin-mediated phosphorylation at S-256; R → Q: in NDI2; exerts a dominant-negative effect on wild-type-AQP2 in that it interferes with its trafficking to the apical membrane; is a loss of function instead of a gain of function mutation on dominant nephrogenic diabetes insipidus
256 modified: Phosphoserine; by PKA; S→A: Retained in vesicles.; S→D: Expressed in the apical membrane.
258 E → K: in NDI2; retained in the Golgi compartment; dbSNP:rs104894332
262 P → L: in NDI2; mutant protein folds properly and is functional but is retained in intracellular vesicles; able to assemble into tetramers with wild-type AQP2 that properly localize to the apical membrane; dbSNP:rs104894339; P→A: No effect on expression at the apical cell membrane.

4nefA X-ray structure of human aquaporin 2 (see paper)
32% identity, 94% coverage: 2:243/258 of query aligns to 6:221/239 of 4nefA

query
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binding zinc ion: C74 (≠ L89), H79 (≠ K94)

P55064 Aquaporin-5; AQP-5 from Homo sapiens (Human) (see 3 papers)
32% identity, 100% coverage: 2:258/258 of query aligns to 8:258/265 of P55064

query
sites
P55064

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